LOCUS       DACDCV010000001       128924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDCV010000001 DACDCV010000000
VERSION     DACDCV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.001"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IV041_000001"
     CDS             complement(12..1535)
                     /locus_tag="IV041_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8380828.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IV041_000002"
     CDS             1787..2968
                     /locus_tag="IV041_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8380829.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV041_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV041_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8380830.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IV041_000004"
     CDS             complement(5053..7764)
                     /locus_tag="IV041_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8380831.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IV041_000005"
     CDS             complement(7774..8475)
                     /locus_tag="IV041_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380832.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IV041_000006"
     CDS             8661..9260
                     /locus_tag="IV041_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8380833.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IV041_000007"
     CDS             9341..10354
                     /locus_tag="IV041_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8380834.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IV041_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IV041_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8380835.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IV041_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IV041_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8380836.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IV041_000010"
     CDS             11802..13175
                     /locus_tag="IV041_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8380837.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IV041_000011"
     CDS             13532..15676
                     /locus_tag="IV041_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8380838.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IV041_000012"
     CDS             complement(15728..16768)
                     /locus_tag="IV041_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8380839.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IV041_000013"
     CDS             complement(17045..18847)
                     /locus_tag="IV041_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8380840.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="IV041_000014"
     CDS             complement(18861..19613)
                     /locus_tag="IV041_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380841.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="IV041_000015"
     CDS             complement(19622..19984)
                     /locus_tag="IV041_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8380842.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="IV041_000016"
     CDS             complement(20160..21032)
                     /locus_tag="IV041_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8380843.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="IV041_000017"
     CDS             complement(21058..21570)
                     /locus_tag="IV041_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8380844.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22538)
                     /locus_tag="IV041_000018"
     CDS             complement(21708..22538)
                     /locus_tag="IV041_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8380845.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22554..23162)
                     /locus_tag="IV041_000019"
     CDS             complement(22554..23162)
                     /locus_tag="IV041_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8380846.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="IV041_000020"
     CDS             complement(23155..23472)
                     /locus_tag="IV041_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8380847.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="IV041_000021"
     CDS             complement(23628..24356)
                     /locus_tag="IV041_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8380848.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="IV041_000022"
     CDS             24515..25396
                     /locus_tag="IV041_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8380849.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="IV041_000023"
     CDS             complement(25633..26676)
                     /locus_tag="IV041_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8380850.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="IV041_000024"
     CDS             complement(26679..27347)
                     /locus_tag="IV041_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8380851.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="IV041_000025"
     CDS             27489..29825
                     /locus_tag="IV041_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8380852.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="IV041_000026"
     CDS             complement(29940..30251)
                     /locus_tag="IV041_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380853.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="IV041_000027"
     CDS             30432..31907
                     /locus_tag="IV041_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8380854.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="IV041_000028"
     CDS             31954..32538
                     /locus_tag="IV041_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8380855.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="IV041_000029"
     CDS             32540..32959
                     /locus_tag="IV041_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8380856.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="IV041_000030"
     CDS             complement(33240..33524)
                     /locus_tag="IV041_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8380857.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="IV041_000031"
     CDS             complement(33876..34586)
                     /locus_tag="IV041_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8380858.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="IV041_000032"
     CDS             complement(34583..36055)
                     /locus_tag="IV041_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8380859.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="IV041_000033"
     CDS             36193..36666
                     /locus_tag="IV041_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8380860.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="IV041_000034"
     CDS             complement(36819..37370)
                     /locus_tag="IV041_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8380861.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="IV041_000035"
     CDS             37475..38005
                     /locus_tag="IV041_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8380862.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="IV041_000036"
     CDS             complement(38488..38931)
                     /locus_tag="IV041_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8380863.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="IV041_000037"
     CDS             complement(38947..40107)
                     /locus_tag="IV041_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8380864.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="IV041_000038"
     CDS             40614..42314
                     /locus_tag="IV041_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380865.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="IV041_000039"
     CDS             complement(42416..43249)
                     /locus_tag="IV041_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8380866.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="IV041_000040"
     CDS             complement(43553..45718)
                     /locus_tag="IV041_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8380867.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="IV041_000041"
     CDS             45978..48272
                     /locus_tag="IV041_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8380868.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="IV041_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="IV041_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8380869.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="IV041_000043"
     CDS             48923..49930
                     /locus_tag="IV041_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8380870.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="IV041_000044"
     CDS             49952..50971
                     /locus_tag="IV041_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8380871.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IV041_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IV041_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8380872.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="IV041_000046"
     CDS             complement(52729..54150)
                     /locus_tag="IV041_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8380873.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IV041_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IV041_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8380874.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="IV041_000048"
     CDS             complement(56561..57817)
                     /locus_tag="IV041_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8380875.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58397..59698
                     /locus_tag="IV041_000049"
     CDS             58397..59698
                     /locus_tag="IV041_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8380876.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="IV041_000050"
     CDS             complement(59767..60366)
                     /locus_tag="IV041_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8380877.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="IV041_000051"
     CDS             60620..61501
                     /locus_tag="IV041_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380878.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="IV041_000052"
     CDS             61514..63124
                     /locus_tag="IV041_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8380879.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="IV041_000053"
     CDS             complement(63201..63860)
                     /locus_tag="IV041_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8380880.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="IV041_000054"
     CDS             complement(64143..64808)
                     /locus_tag="IV041_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380881.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IV041_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IV041_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8380882.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IV041_000056"
     CDS             complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IV041_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8380883.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IV041_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IV041_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8380884.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="IV041_000058"
     CDS             67122..67442
                     /locus_tag="IV041_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8380885.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="IV041_000059"
     CDS             complement(67554..68423)
                     /locus_tag="IV041_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8380886.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69793)
                     /locus_tag="IV041_000060"
     CDS             complement(68519..69793)
                     /locus_tag="IV041_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8380887.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVETILGYGSGTWKSPERTKK"
     gene            complement(69819..70328)
                     /locus_tag="IV041_000061"
     CDS             complement(69819..70328)
                     /locus_tag="IV041_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380888.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="IV041_000062"
     CDS             complement(70503..72638)
                     /locus_tag="IV041_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8380889.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="IV041_000063"
     CDS             complement(72721..74376)
                     /locus_tag="IV041_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8380890.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="IV041_000064"
     CDS             complement(74378..74692)
                     /locus_tag="IV041_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8380891.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="IV041_000065"
     CDS             74862..75122
                     /locus_tag="IV041_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8380892.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="IV041_000066"
     CDS             75136..75450
                     /locus_tag="IV041_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8380893.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="IV041_000067"
     CDS             complement(76180..76734)
                     /locus_tag="IV041_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8380894.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="IV041_000068"
     CDS             complement(76860..78788)
                     /locus_tag="IV041_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8380895.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="IV041_000069"
     CDS             complement(79094..79810)
                     /locus_tag="IV041_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8380896.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IV041_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IV041_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8380897.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IV041_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IV041_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8380898.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="IV041_000072"
     CDS             complement(83280..84740)
                     /locus_tag="IV041_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8380899.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="IV041_000073"
     CDS             complement(84730..86025)
                     /locus_tag="IV041_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8380900.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="IV041_000074"
     CDS             complement(86357..87034)
                     /locus_tag="IV041_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8380901.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="IV041_000075"
     CDS             complement(87064..88155)
                     /locus_tag="IV041_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8380902.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="IV041_000076"
     CDS             complement(88256..88690)
                     /locus_tag="IV041_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8380903.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IV041_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IV041_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8380904.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="IV041_000078"
     CDS             complement(90728..92623)
                     /locus_tag="IV041_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8380905.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="IV041_000079"
     CDS             complement(92681..94456)
                     /locus_tag="IV041_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8380906.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="IV041_000080"
     CDS             complement(94506..96002)
                     /locus_tag="IV041_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8380907.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="IV041_000081"
     CDS             complement(96196..97818)
                     /locus_tag="IV041_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8380908.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="IV041_000082"
     CDS             complement(98267..99580)
                     /locus_tag="IV041_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8380909.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="IV041_000083"
     CDS             complement(99825..100049)
                     /locus_tag="IV041_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380910.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100437)
                     /locus_tag="IV041_000084"
     CDS             complement(100069..100437)
                     /locus_tag="IV041_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8380911.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="IV041_000085"
     CDS             complement(100635..101345)
                     /locus_tag="IV041_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380912.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="IV041_000086"
     CDS             complement(101363..101860)
                     /locus_tag="IV041_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8380913.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="IV041_000087"
     CDS             complement(101897..102349)
                     /locus_tag="IV041_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8380914.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="IV041_000088"
     CDS             complement(102484..103089)
                     /locus_tag="IV041_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8380915.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="IV041_000089"
     CDS             complement(103539..104159)
                     /locus_tag="IV041_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8380916.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="IV041_000090"
     CDS             complement(104161..105030)
                     /locus_tag="IV041_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8380917.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="IV041_000091"
     CDS             complement(105053..105460)
                     /locus_tag="IV041_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8380918.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="IV041_000092"
     CDS             complement(105479..106768)
                     /locus_tag="IV041_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8380919.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="IV041_000093"
     CDS             complement(106795..107097)
                     /locus_tag="IV041_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8380920.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="IV041_000094"
     CDS             complement(107122..109215)
                     /locus_tag="IV041_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8380921.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="IV041_000095"
     CDS             complement(109704..110468)
                     /locus_tag="IV041_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8380922.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IV041_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IV041_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8380923.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="IV041_000097"
     CDS             complement(111473..112549)
                     /locus_tag="IV041_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8380924.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="IV041_000098"
     CDS             complement(112573..113166)
                     /locus_tag="IV041_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8380925.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IV041_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IV041_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8380926.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="IV041_000100"
     CDS             complement(114836..115135)
                     /locus_tag="IV041_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8380927.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IV041_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IV041_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8380928.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="IV041_000102"
     CDS             complement(116890..117801)
                     /locus_tag="IV041_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8380929.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="IV041_000103"
     CDS             117921..118778
                     /locus_tag="IV041_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8380930.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="IV041_000104"
     CDS             complement(118965..119327)
                     /locus_tag="IV041_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380931.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="IV041_000105"
     CDS             119673..121112
                     /locus_tag="IV041_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8380932.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="IV041_000106"
     CDS             complement(121119..123671)
                     /locus_tag="IV041_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8380933.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="IV041_000107"
     CDS             complement(123785..125116)
                     /locus_tag="IV041_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8380934.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="IV041_000108"
     CDS             complement(125082..126560)
                     /locus_tag="IV041_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8380935.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IV041_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IV041_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8380936.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37724 a        26960 c        22544 g        41696 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgtttcga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaa
//
LOCUS       DACDCV010000002        86371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDCV010000002 DACDCV010000000
VERSION     DACDCV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.002"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            115..1239
                     /locus_tag="IV041_000110"
     CDS             115..1239
                     /locus_tag="IV041_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8380937.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1466..2383
                     /locus_tag="IV041_000111"
     CDS             1466..2383
                     /locus_tag="IV041_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8380938.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2376..3209
                     /locus_tag="IV041_000112"
     CDS             2376..3209
                     /locus_tag="IV041_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8380939.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3292..4410
                     /locus_tag="IV041_000113"
     CDS             3292..4410
                     /locus_tag="IV041_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8380940.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4404..6302
                     /locus_tag="IV041_000114"
     CDS             4404..6302
                     /locus_tag="IV041_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8380941.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6296..7519
                     /locus_tag="IV041_000115"
     CDS             6296..7519
                     /locus_tag="IV041_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8380942.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7605..9539)
                     /locus_tag="IV041_000116"
     CDS             complement(7605..9539)
                     /locus_tag="IV041_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8380943.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9765..10421
                     /locus_tag="IV041_000117"
     CDS             9765..10421
                     /locus_tag="IV041_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8380944.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10495..10848)
                     /locus_tag="IV041_000118"
     CDS             complement(10495..10848)
                     /locus_tag="IV041_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8380945.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11040..11969
                     /locus_tag="IV041_000119"
     CDS             11040..11969
                     /locus_tag="IV041_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8380946.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11980..12816
                     /locus_tag="IV041_000120"
     CDS             11980..12816
                     /locus_tag="IV041_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8380947.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13064..13840
                     /locus_tag="IV041_000121"
     CDS             13064..13840
                     /locus_tag="IV041_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8380948.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14016..14759
                     /locus_tag="IV041_000122"
     CDS             14016..14759
                     /locus_tag="IV041_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380949.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14752..16332
                     /locus_tag="IV041_000123"
     CDS             14752..16332
                     /locus_tag="IV041_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8380950.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16583..17062)
                     /locus_tag="IV041_000124"
     CDS             complement(16583..17062)
                     /locus_tag="IV041_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8380951.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17078..17830)
                     /locus_tag="IV041_000125"
     CDS             complement(17078..17830)
                     /locus_tag="IV041_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8380952.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17947..18017
                     /locus_tag="IV041_000126"
     tRNA            17947..18017
                     /locus_tag="IV041_000126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17979..17981,aa:Gly,seq:ccc)
     gene            18378..19103
                     /locus_tag="IV041_000127"
     CDS             18378..19103
                     /locus_tag="IV041_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8380953.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19358..19624
                     /locus_tag="IV041_000128"
     CDS             19358..19624
                     /locus_tag="IV041_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8380954.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19621..20052
                     /gene="ruvX"
                     /locus_tag="IV041_000129"
     CDS             19621..20052
                     /gene="ruvX"
                     /locus_tag="IV041_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8380955.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20077..20382
                     /locus_tag="IV041_000130"
     CDS             20077..20382
                     /locus_tag="IV041_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8380956.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20463..21608)
                     /locus_tag="IV041_000131"
     CDS             complement(20463..21608)
                     /locus_tag="IV041_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8380957.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21669..22316)
                     /locus_tag="IV041_000132"
     CDS             complement(21669..22316)
                     /locus_tag="IV041_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8380958.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22510..22803
                     /locus_tag="IV041_000133"
     CDS             22510..22803
                     /locus_tag="IV041_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380959.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22847..23158
                     /locus_tag="IV041_000134"
     CDS             22847..23158
                     /locus_tag="IV041_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380960.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23155..23779
                     /locus_tag="IV041_000135"
                     /pseudo
     CDS             23155..23779
                     /locus_tag="IV041_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24079..24441
                     /locus_tag="IV041_000136"
     CDS             24079..24441
                     /locus_tag="IV041_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380961.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24478..25326
                     /locus_tag="IV041_000137"
     CDS             24478..25326
                     /locus_tag="IV041_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8380962.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25316..26791
                     /locus_tag="IV041_000138"
     CDS             25316..26791
                     /locus_tag="IV041_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8380963.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            27057..27464
                     /locus_tag="IV041_000139"
     CDS             27057..27464
                     /locus_tag="IV041_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8380964.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27467..27682
                     /locus_tag="IV041_000140"
     CDS             27467..27682
                     /locus_tag="IV041_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380965.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27686..28066
                     /locus_tag="IV041_000141"
     CDS             27686..28066
                     /locus_tag="IV041_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8380966.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28200..28355
                     /locus_tag="IV041_000142"
     CDS             28200..28355
                     /locus_tag="IV041_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8380967.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28424..28897
                     /locus_tag="IV041_000143"
     CDS             28424..28897
                     /locus_tag="IV041_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8380968.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28894..30588
                     /locus_tag="IV041_000144"
     CDS             28894..30588
                     /locus_tag="IV041_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8380969.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30554..30739
                     /locus_tag="IV041_000145"
     CDS             30554..30739
                     /locus_tag="IV041_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380970.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30743..31918
                     /locus_tag="IV041_000146"
     CDS             30743..31918
                     /locus_tag="IV041_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8380971.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31911..33434
                     /locus_tag="IV041_000147"
     CDS             31911..33434
                     /locus_tag="IV041_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8380972.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33490..33774
                     /locus_tag="IV041_000148"
     CDS             33490..33774
                     /locus_tag="IV041_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8380973.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33761..34096
                     /locus_tag="IV041_000149"
     CDS             33761..34096
                     /locus_tag="IV041_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8380974.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34391..35017
                     /locus_tag="IV041_000150"
     CDS             34391..35017
                     /locus_tag="IV041_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380975.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35759..36596
                     /locus_tag="IV041_000151"
                     /pseudo
     CDS             35759..36596
                     /locus_tag="IV041_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            36661..37095
                     /locus_tag="IV041_000152"
     CDS             36661..37095
                     /locus_tag="IV041_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8380976.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(37163..38485)
                     /locus_tag="IV041_000153"
     CDS             complement(37163..38485)
                     /locus_tag="IV041_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8380977.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38639..40279)
                     /locus_tag="IV041_000154"
     CDS             complement(38639..40279)
                     /locus_tag="IV041_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8380978.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40609..41079
                     /locus_tag="IV041_000155"
     CDS             40609..41079
                     /locus_tag="IV041_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8380979.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            41708..42811
                     /locus_tag="IV041_000156"
     CDS             41708..42811
                     /locus_tag="IV041_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8380980.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            43095..43775
                     /locus_tag="IV041_000157"
     CDS             43095..43775
                     /locus_tag="IV041_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8380981.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43865..44899
                     /locus_tag="IV041_000158"
     CDS             43865..44899
                     /locus_tag="IV041_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8380982.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44913..45248
                     /locus_tag="IV041_000159"
     CDS             44913..45248
                     /locus_tag="IV041_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380983.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            45232..47712
                     /locus_tag="IV041_000160"
     CDS             45232..47712
                     /locus_tag="IV041_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8380984.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            47768..49327
                     /locus_tag="IV041_000161"
     CDS             47768..49327
                     /locus_tag="IV041_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8380985.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            49324..50688
                     /locus_tag="IV041_000162"
     CDS             49324..50688
                     /locus_tag="IV041_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8380986.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50859..51929
                     /locus_tag="IV041_000163"
     CDS             50859..51929
                     /locus_tag="IV041_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8380987.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51944..52612
                     /locus_tag="IV041_000164"
     CDS             51944..52612
                     /locus_tag="IV041_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380988.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52632..53210
                     /locus_tag="IV041_000165"
     CDS             52632..53210
                     /locus_tag="IV041_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8380989.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53613..54524
                     /locus_tag="IV041_000166"
     CDS             53613..54524
                     /locus_tag="IV041_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8380990.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54780..55697)
                     /locus_tag="IV041_000167"
     CDS             complement(54780..55697)
                     /locus_tag="IV041_000167"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8380991.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55899..56336
                     /gene="zapA"
                     /locus_tag="IV041_000168"
     CDS             55899..56336
                     /gene="zapA"
                     /locus_tag="IV041_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8380992.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56394..56941
                     /locus_tag="IV041_000169"
                     /pseudo
     CDS             56394..56941
                     /locus_tag="IV041_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            57056..59416
                     /locus_tag="IV041_000170"
     CDS             57056..59416
                     /locus_tag="IV041_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8380993.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59503..59817
                     /gene="trxA"
                     /locus_tag="IV041_000171"
     CDS             59503..59817
                     /gene="trxA"
                     /locus_tag="IV041_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8380994.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59999..61777
                     /gene="uvrC"
                     /locus_tag="IV041_000172"
     CDS             59999..61777
                     /gene="uvrC"
                     /locus_tag="IV041_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8380995.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61922..62821
                     /locus_tag="IV041_000173"
     CDS             61922..62821
                     /locus_tag="IV041_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8380996.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62814..64076
                     /locus_tag="IV041_000174"
     CDS             62814..64076
                     /locus_tag="IV041_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8380997.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            64239..66137
                     /locus_tag="IV041_000175"
     CDS             64239..66137
                     /locus_tag="IV041_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8380998.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(66436..67155)
                     /locus_tag="IV041_000176"
     CDS             complement(66436..67155)
                     /locus_tag="IV041_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8380999.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            67492..67824
                     /locus_tag="IV041_000177"
     CDS             67492..67824
                     /locus_tag="IV041_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381000.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67811..69814
                     /locus_tag="IV041_000178"
     CDS             67811..69814
                     /locus_tag="IV041_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8381001.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69833..70303
                     /locus_tag="IV041_000179"
     CDS             69833..70303
                     /locus_tag="IV041_000179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8381002.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            70324..70908
                     /locus_tag="IV041_000180"
     CDS             70324..70908
                     /locus_tag="IV041_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8381003.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70923..71909
                     /locus_tag="IV041_000181"
     CDS             70923..71909
                     /locus_tag="IV041_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8381004.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            71899..72210
                     /locus_tag="IV041_000182"
     CDS             71899..72210
                     /locus_tag="IV041_000182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8381005.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            72232..74013
                     /locus_tag="IV041_000183"
     CDS             72232..74013
                     /locus_tag="IV041_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8381006.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            74006..75382
                     /locus_tag="IV041_000184"
     CDS             74006..75382
                     /locus_tag="IV041_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8381007.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75389..76024
                     /locus_tag="IV041_000185"
     CDS             75389..76024
                     /locus_tag="IV041_000185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8381008.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            76054..77409
                     /locus_tag="IV041_000186"
     CDS             76054..77409
                     /locus_tag="IV041_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8381009.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77601..78995)
                     /locus_tag="IV041_000187"
     CDS             complement(77601..78995)
                     /locus_tag="IV041_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8381010.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            79087..80058
                     /locus_tag="IV041_000188"
     CDS             79087..80058
                     /locus_tag="IV041_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8381011.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            80055..80837
                     /locus_tag="IV041_000189"
     CDS             80055..80837
                     /locus_tag="IV041_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8381012.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(81006..81377)
                     /locus_tag="IV041_000190"
     CDS             complement(81006..81377)
                     /locus_tag="IV041_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8381013.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81511..81954
                     /locus_tag="IV041_000191"
     CDS             81511..81954
                     /locus_tag="IV041_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8381014.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81997..82806)
                     /gene="yidA"
                     /locus_tag="IV041_000192"
     CDS             complement(81997..82806)
                     /gene="yidA"
                     /locus_tag="IV041_000192"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8381015.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82825..84192)
                     /locus_tag="IV041_000193"
     CDS             complement(82825..84192)
                     /locus_tag="IV041_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8381016.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(84176..85006)
                     /locus_tag="IV041_000194"
     CDS             complement(84176..85006)
                     /locus_tag="IV041_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8381017.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            85169..85606
                     /locus_tag="IV041_000195"
     CDS             85169..85606
                     /locus_tag="IV041_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8381018.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85682..86302
                     /locus_tag="IV041_000196"
     CDS             85682..86302
                     /locus_tag="IV041_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8381019.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        28486 a        14610 c        17898 g        25377 t
ORIGIN      
        1 gccaaaacga caagtaaaat atttttttat ataaatattt gtgttactaa ttccgttaat
       61 atacataagt gaaagcgttt tatataagaa cttgaaaaga aagaagggat agacatgaaa
      121 aaaagataca aattggtgat agctttgcta aacattaccc tattattcag cttaagtgca
      181 tgtggaaaaa ctaacgaaga agtaacagca aacatcgcag atccaatgat aaaaaaagcc
      241 actacattag aagtatatgt cgcaaacggc aaactgacaa actatgttga aaatgctgca
      301 aaaatctata accgcaaaac tggagcaaat attgacttaa aaattgtgaa tgtagcttcg
      361 ggaacagcaa cagtacaaat gatcacccca aaattggtaa cacacgaaga tatgccggat
      421 attatctcca tggcagacac ttcagcagca ggtgtgctgg aaaaatttca agatgctttt
      481 tatagtgctg atgaatatgg attttatgac aaatatggcg atcagtttta tgatcaaaaa
      541 ttgaatattt tgcgtcaaca aacgagtaat gaaacgatta ttccttgggc tagtgatttt
      601 accccagcac tttgttatta tcaaccaaaa aaatttgctg aaataggtgt agattttgaa
      661 gaaatcagtt cttgggaaga atatatccag gtggcaaaag cattaaaaga aaaaactggt
      721 agttttggaa ttgccttgcc agaatctggg gaccaagaat tgtttatcaa catgctagcc
      781 cagcagaatc aatctttgct tgatgaagaa ggaaatatcc gtctgaatac taaagaagcg
      841 aaacatgctg caaagttaat caaagaaatg atcgactcag atattgtaca tttctacggt
      901 gcacaagatg ctgagaaagc atttcaagaa tctgcaatgt ttgttgctgg cggctggtat
      961 gctacgaata tgtctttgaa ttttcctgat gcatctgaca aatggcgcat ggctccactg
     1021 attccttttt caaaagaaaa tccaggaaaa ggtgcagtat caggaggatc aagtttttat
     1081 gtccctaaag atgccaaaaa tccacaagtg gcacaacaat ttcttacatt tatgctgaca
     1141 gatgaagaat gcttagccaa cgcactggag ctgggcgtgg ctacatctaa ctcattggcc
     1201 tatcaaacag aagcagctga gaaaaagttt gactactaag gaggacaaaa atactatgaa
     1261 ctattgaata aaatcaatga aaatacatca aatactgttt taccagccac ttattcagat
     1321 gctacgtctt atttgactac cgctgcatat aactattggc aaacaggtga ttttgaaaat
     1381 tcatatctaa aagaagcaga aaattttgct cataagtacg gcgtaaaatt aactggtgat
     1441 taaaaaatat taggaggact taacgatgat gcaatcttcg ttaccaagca agacaataaa
     1501 gaacagcaag aaaaaagcaa cagatacgtc tcataaaaaa agtgcttatg gattggtcac
     1561 accttttgtt attttcttta ttgtgacgat tttgattccc atcattatct cgtttgcttt
     1621 tagttttatg aaaattggct actcatttga atttgttgga tttgacaatt atctagctat
     1681 cttccgagat ccattgttta ttaaatctta cacgaatgtt ttgattatca tggcaggatc
     1741 aattccaata acgatgctat tagctattta ctttgctgtt ttattgaatt cagcacagtt
     1801 aaaaggaaaa ggatttttta gaactgtcta ttatattcca accattacat caacggtggc
     1861 tgtagcttcc gtattcatga cttttttcaa tccgactggg ttattcaatg gatttttgaa
     1921 tgcgctagga attaacggtg tacagtggtt aacaaatcct ttttggatac gaatatcaat
     1981 gatcatcacg atgatttggc ttagtgtagg atataacact attttatttt tagctggtct
     2041 tcaagggatt tctaaagaga tatacgaaag tgctgaaata gatggagcat cgaaatttcg
     2101 gcaattcttt ttattaacga tccccatgct aaagccaatc attttaatgg ccacagtttt
     2161 agccactatc aatggcttgg gtaatttcaa tatcccaaat atattctttg ggacttccaa
     2221 tggaccagaa aattcagctt tggtcgtagg agtcaattta tataaaacaa gttttgaaat
     2281 ggttgatttt ggaaaagcct ctgctattgc ctggacgatg gtgtttgtat ctgttatatt
     2341 atcggctatt caatttaaat tcggaggtga taaaaatgac taaacaaaag atacaaagca
     2401 gaatttttac gtacgtcatt attgtgtttg gtgccttaat atttttgatg ccatatttat
     2461 atatgattgt agcttcaacg caaagtaatc aagaaatttt aagtggcgga atcaatttca
     2521 agtttgggga tatgctatta aaaaactggc aagccatcat ggcgagatat aattatccct
     2581 tgattatttg gaattcgatt gttattgctg taatcagtac agttttagct acagccagtt
     2641 ctacaatggc gggttatgca ttagcaaagt atcgttttat ggggaataaa ctgttgttca
     2701 atacagttat gctatctagg atggtgcctt tttttaccac attgattcct ttattttaca
     2761 ttatgagtcg tttaggatta tcgaatactt atgcaggtgt gatcatccct tcgattgcca
     2821 gtacaacttc agtgtttatg atgcgacaat atagcagtca atttcctaca gcactgatgg
     2881 aggctgccag aatcgatggt gccaatgagt gggtgatttt tacgaaaatc gctgtaccga
     2941 ttttatcacc tacgattatt actagtggtt tacttatctt tatgggaagt tggaatcaat
     3001 atctatttcc attagtcatg ctttcagata aagataaata tacggttccc ttaattatca
     3061 aaaatatgac ggtttctagt acaggtgagc caattaatta tggcgcaatt atgctaattt
     3121 tagcaacttc agtgatccca atggtcttga tttatgtgtg ggcgcagtct aaatttaagg
     3181 agaatgacat tggagctgca aataagtaaa aaaaataacg aatgttaacc atctagttcg
     3241 ttctaggagc aactccacat tcataaaaat taaaagaatg gcgggatatc catgaatgaa
     3301 cagacgaagg aatggtcaag aaaaattttg aatgtagctt ctgaaaagat agatgcggaa
     3361 ttagacagaa tggggaataa aattccatat atttcttaca acggaaaata tacagacgta
     3421 ataaaggaca gaggaatcga ctggtggacc aatggttttt ggagtggtat tttatggcag
     3481 ctctttcaca agacaaaacg agaaaagttc aaagaggcag caatacaaca agaacaatta
     3541 ttagaatctg ccctgtacca tttttcagac gttcatcatg atgtaggttt tttattctta
     3601 ccatcggcta ttgcacatta tcaacagtct caaaacaaac atagtcgaca actagggtta
     3661 catgcagcta cggtacttgc tggacgctat aatatttcag ggaactattt agtagcatgg
     3721 aatgaccata aacctggttg gatgattatt gatagtatga tgaatttacc attactgtat
     3781 tgggcttcga gggaaactgg tgatccgagg ttctctaaaa ttgctcggca gcatgcagat
     3841 acaacagcta agtatctagt gaggaaagat ggatcaaccg ggcatatcgc agaatttgat
     3901 gcagaatcag ggaaatatat tcggttactt ggcgggcaag gatacaatga gaattcttgt
     3961 tggagcagag gaaatgcatg ggctttatat ggattcgcgt taagttatcg ttacacaaaa
     4021 aatccgtact atctagctat agcagaaaag atagcagtaa attttattca gaaaatcgaa
     4081 aaaactggtt atattcctct cattgatttt gctgtaaaag attcgaatat tgataccagt
     4141 gctggagtat gcgctgcatg cggtcttctg gaattagcag gatatgtttc ctctgtacga
     4201 tcaaaaatgt atcatgaagc tgctatcaat atactccaaa agacagaaaa gcactatgca
     4261 aattggaata tggatgaaga tgggatcatt ggaggcggag cagaagcata ccatagagaa
     4321 ctgacttata atgtgccact gatctattct gattattttt ttattgaagg cttacttcga
     4381 ttagaacagt cggaattagc tatttggtag taaaaagagg agaaaataca atgaaacatc
     4441 cagtaccact attaaggacc agacaggatt actttgactt atttaaaaag ataaatcaac
     4501 cactccagcc gttttatcga gcaagtggcg cacaaattga ttatatacat caaggggtag
     4561 gatatggaaa taaaattgca ggaatggaag gattctctcg ggttttatgg ggagcggggc
     4621 ccgcaattga ccaattagat gaacgatggt tgaaattaat tctatcagga ataaaagctg
     4681 gaacagatcc ttcccatccc gattattggg gagaaataca tgaaagagac cagcgtatgg
     4741 tagaaatgcc cgcaatgtta ttagcgttat atcatcatga acaattatta tggaaaaaat
     4801 tagatacagg agaaaaaagc cagattattc attggttctc gcaaattttt gaatatgaat
     4861 gtgcagatgg taattggcaa ttttttaaga taattgtcgg aacaattatc caaaaatttg
     4921 gttatccagt taaagaatca gcactgaaag aagcatttga taatatagaa aattgttatc
     4981 ttcaaaatgg ctggtatcga gacagttcaa gaggtcgcca agattattat aatccttttg
     5041 catttcatta ttatggtttg atttatagcg tactagaacc agaaaagccc attagccaga
     5101 tatataagaa ccgagctatt gatttttcta gagactatat tcattttttt gcggaagatg
     5161 gtgcaaatgt gccttttggt cgaagcatga tttatcgttt tgcagtttca agcttttggt
     5221 cagctatgtt gtgggccgat cttttgcctt ttaaattagg tgaaatcaag gggttagtca
     5281 atcggaatat aagatggtgg atgcaaaaag aaatatttga tgaaaaaggt atcctgaata
     5341 ttggttatac ctaccctcaa ttgacattga ctgaacccta taatagtacg ttatctccat
     5401 tatggctgaa taagattttt ttattgttgg cgctacctga agaccatgag tattggacgg
     5461 ctaaagaaat gcctatgcct attcgtgaag gaactaaact tctttcagaa gccaatttgc
     5521 tcatacaaca tgataatgga catacatttt ttttgaatgc gggacaatta gggccaaatt
     5581 tccatacatt gaccaatgaa aaatatttaa aatttgctta ttctagtcaa tttggttttt
     5641 cgataccgcg agcgaatcaa ttgaaagcag aaatggcaat ggattcaatg ctagggattc
     5701 agcgagcgga cacatatatc acgaccagtt ttaagaatga gagcatcaaa acctatggac
     5761 agttcatcgt tcgaaataat gtacgagaca ttgtatgtaa gaaagaggta cttgcctcta
     5821 catgggaacc tactttacag gcaaaaatca gaacttggct tattcctttt gaaggctggc
     5881 agattcgcgt acacaaagta gaattagata cagaatatgt tctatatgag acagggtttg
     5941 ctattgaatg ttctcctgag aaagaaggaa taatcataga agaagacaag gaaaactata
     6001 gggtgagtga agctggcttt tcaggtatta tctgtttaag tgacgatact attaaacgaa
     6061 aatccacagc tattatgggt ttgcccaata caaacttgat gacatgggaa aacactttta
     6121 ttcctggatt agagggtaca tttggaaaag gaacccattg gctcggaaca ggagtggttg
     6181 ctcatcaaga tcgggattat agtttagaag tatggaacaa tcgtcccaaa attgattttg
     6241 atgggacaac cgggctaact atccaaaata aaaacaataa aaaaagacta aaattatgct
     6301 aaaggctggt ggggaaatgc ttgataaagt gatgtttgaa gagataatac atccagaaaa
     6361 atggataaat gaatgttata aagaattaat ggaaaaagaa tggaataatg agatcgagcg
     6421 ttgtcgcaga cacacggaga ggttaatcaa acgtatcagt aacagtcagt atcaattccc
     6481 aagcgagtgt cctataaaag gacgttatcc attaagagac aatattggtt ggacagaagg
     6541 attttggaca ggaattaatt ggttgttatt tgagaagacg ggtgatcctt tatttcaaca
     6601 aacagctgaa aaagcagata tagattttat ccacaggatt gactgtcaaa ttatcactaa
     6661 tcatcacgat cttggttttt tgtattcttt atcaaccgta gcagattata aaataacggg
     6721 aaatcgaaaa atgaaggaag cttcaattga tgctgcagaa acattacttc agcgtttttg
     6781 ggagaaggca ggagttatac aagcttgggg agatcctagt gatccttcac aacaaggtag
     6841 aatgatcatg gattgttgca tgaatcttcc attattgtat tgggcgagtg aagaaactgg
     6901 aaatcaaaaa tataaaaaag ctgctcaggc tcatattcaa aatgcagcag attatttagt
     6961 aagagaagat ggttcaactt atcatacatt ttatatggat gtcaaagatg gaaaaccaag
     7021 atatggaagt acgcatcaag gttatagtga taattcctgt tgggctagag gccaagcatg
     7081 ggggatttac ggttttgcat taagttatcg ttatttgaaa gaacaaaaat attttgattt
     7141 aagcaaaata ttagccaatt attttatgaa tcggttgcca gaagatacca tttgttattg
     7201 ggatttgatc tttgatgatt ctagcagaga agaaaaagat acatctagta tggtgatcat
     7261 ggtttgtgga ttattagaat taatagaatc aggtctatta gacaaaacag aaaagaactt
     7321 ataccagtct gctgtttgga aaattatgcg acaattgaaa gaaagatatg ctacaacgga
     7381 agaagaagat ggctttttaa cacagtctgt ttataataag cccaaaaata atggggtaaa
     7441 tacaccttgt atttggggtg attactatta tttagaagct ttagtgagaa tgactagtaa
     7501 ttggtcttct tattggtgaa aaaggtaaat atggaatttt tgtttgataa aataaaaaaa
     7561 gaaaaaccaa aacagcatga atggtttctg ttttggtttt ttcgttaatt ttcatattct
     7621 tctaattcca agcttagttc ttcccattcc gtcaattttg tttcttgatc ttgtctagca
     7681 gcttctaaat cttggttcag ctcattgagt tttacgtgat cttccaggtt ttctggcgcg
     7741 accatttctt gttccaactg atcgatccat tcatcgatcc gcgccatttc ttcttcaacc
     7801 tgagtaattt ttcgttgaag gctacggata agcttttgct gttctttgct ttggtaataa
     7861 tcagatttgt tcgtgggagc aggagcagat tctggctcat cttgtgccaa caattcggcg
     7921 atttcttctt cttcctgctt tttctctaaa taataatcat agtctcctag atagactttg
     7981 ctgccatttt cagagagttc gacgactttt gtcgcgatcc gattgatgaa atatcggtca
     8041 tgggaaacaa aaagcagtgt gccgtcataa tcgatcaatg cattttctaa tacttctttg
     8101 ttgtcaatat ctaaatggtt tgttggttca tccaaaatca aaaaattttc tttgttcatc
     8161 gctaacttgg ctaaagcaac ccgtgctttt tcaccgccac ttagcagagg gattggtttt
     8221 tctacatctt cgccagaaaa taagaaagca cctaaagcac ttcggatctc tttttctgga
     8281 gtggtaggat gctcatccca caattctgct aggatcgtct tgtttgtatg cagatcagct
     8341 tgaccttgat cgtaatagcc gatggtgaca tttgtcccta gcgtttcttt tccacggata
     8401 aaagggatct ggtgaatgat tgatttcagc aacgtcgatt tcccgatacc attcggacct
     8461 actagagcaa tcgcttcttg acgacggata tctagattga ttggttcaga taaggtgaca
     8521 ttatcatatc cgatagcggc atcttcgatt tgcaagacta catttccaga aggcttttct
     8581 atactgaata gaaagtttgc ttgtttttca tccccttgag gacgatccag ccggtccatt
     8641 ttttctaatg tctttctgcg gctttgcgct cgttttgtgg tagaggcgcg aacaaggttc
     8701 cgagcaacga agtcttctaa tttatggatt tctgtttgct gcttttcata agccttccat
     8761 tcacttgcta actgttctgc tttcaaatct agatatttcg aatagttacc tttgtaatgg
     8821 cgcatttttc ttctgcttaa ttcatagact tcattgacta ctttatctaa aaaataacgg
     8881 tcatgggaaa caatcaataa tgcaccagta tagctttgca gataattctc aagccaggaa
     8941 agtgtctcga tatctaaatg gttcgttggt tcatctagaa tcaaaatgtc agggtgttgc
     9001 aaaagcatgc gagctaaagc taaacgtgtg cgctgtccgc ctgataaagt atcgattttc
     9061 tgatcataaa atgaagaatc gaatttaaac ccatgcaata cggaacgaat ttcattttca
     9121 taaccatagc cgtttcgatc tgaaaaatcg tgttgcagcc ggtcgtattc ttttaaataa
     9181 gaactataag caggtgtgtc tggcgtacct tcgctaatga aaatctctag ctcgcgcatc
     9241 cgctcttcca tttgtctaac ttgttcaaaa gctttcagca tctcgttcca aactgtttca
     9301 ttggaatcaa gtcctgtgtc ctgagcgagg tatccgagag tcgctgtttt atttttagcg
     9361 atctttcctt catcaggtgc ttcaattcca gcaatgatct ttaataaagt cgattttcca
     9421 gcgccatttc tgccaactaa agcgacacga gcgcctgtac tgatttctaa atgtatgttt
     9481 tcaaataaga cttctgcacc aaaatggcga gcaatctgat tggcttgtaa taatatcatg
     9541 gaatcctctt ttctactttg tgaattctcc tcaaataaaa aactattttt caattagtag
     9601 tctaccataa attccattat actgcgagat ttgcagaaat gataaataaa tgaataaaga
     9661 ataaaatttc acaatatctg agcaatggaa ttgatttttt cctataaata ctgctatgat
     9721 ggagaaatca aagatgtaat tagacaatac gtggaggtca tttcgtgaaa gatcatatta
     9781 ttccaaaggc gacagctaaa agactgcctt tgtattatcg ctatttaagg attttaaata
     9841 atgctggaaa gaaaaaagtg tcttcaactg aattaagtga agccgttcaa gtagatagtg
     9901 caacgatccg tcgagatttt tcttattttg gcgaattagg aaaacgtggg tatggttatg
     9961 atgtagaaga tcttatgcac ttttttgcaa agacattaaa cgacgatcaa ttgacaaatg
    10021 ttgcgctgat cggtgtaggg aacttgggaa gtgctttact aaaatacaaa ttccatcaaa
    10081 gtaatagcat acgtatcagt tgtgcttttg atgtgaatga ggaaatcgtt gggcgcatcg
    10141 tagacggtat ccccgtttat ccaatggaag atatgtcaga acaaatcaag atccaacaaa
    10201 tcgaagtagc aattttaacg atacccgctg aaagagcaca agaagtagtc aatcagttaa
    10261 cggaagcagg aatcaaagga atcttgaact ttaccgctgc tcgattgaat gctccgtcag
    10321 atgtattgat ccaaaatgtt gatttgacga atgaattgca aaccttgatc tatttcttgc
    10381 attctgacgc aaatgcgcat tccgaaaata cagcagaata aaaataaaaa gcgattgaac
    10441 aaatgaaggc aaaactttca cttgttcaat cgcttttttc tgtttggaca acgattattt
    10501 tgttcggaag atttcttcat aagaaaggga atcatcttct gctgtcagtt catctagtag
    10561 ataacggatt tttttaaagt cgatgaaccg tgcttgccga aagacttctt ttccttgatg
    10621 aaaaatcagg acaactggcg cagtcagcac ttcaaattta ctagctactt ctggtgtttc
    10681 aaatgcatcc aaatgaagtg ccggaatatc ataatgtgtg agaagttcct ctagtcgtgg
    10741 acgaacagcg tgacaaacac tgcaattagg catcgaaaga taaatgacgg caacggatga
    10801 ctgttgcagt atgtcttgaa cttcagtcaa gttagtggtt tgtttcattt ttattctccc
    10861 ttttatgata gaagtgatat gtatgagacc ttaatttaat gggaataaca gaaaaagtca
    10921 attgaagtgc tttggtaaaa aataagagtt gtgtaaaaat ttacagagca acttgttttt
    10981 tttgaataaa agcgtatatt tgcaaagcga atgaactaga aataaaaagg aggaaactca
    11041 tgttttcttt tattccaaat tcggttttgc agatgagtac gattttctta tcgatcgtta
    11101 ttgaagcatt acctttcgta ttattaggct gtcttatctc aggcgcctta caagtatttt
    11161 tgaccccaga acgtgtgaag agatggcttc ctgaaaatcg tatcttctct atttttactg
    11221 gttcgatctt aggatttttc tttccttcat gtgaatgcgg catcgtaccg atcgttcatc
    11281 aatttgttaa aaaaggcgta ccagttcaca cagcgtttgc ttttatgctg actgctccga
    11341 tcatcaatcc aatcgtgatt ttttccactt ttatcgcatt tggcaattct tggcagatgg
    11401 caagctggcg ggtgataggc agcttttttg ttgcccttgt gattggtgtc tggcttgcct
    11461 attttcaaaa agacgctgta ttgaaagaaa aaatcacgtc taaagtagca gaaaatcacc
    11521 atcatcatgt ccatgaagaa gaacaaagtg agaagaatga accaaaaaat cagctgatcc
    11581 atcaaactgg gcatgtgttg acacattcaa tcgacgaatt ttttgataca ggtcgttatt
    11641 tgatcattgg cggattaatc gcagctgcga tgcagactta tttaccaact agaattatgc
    11701 taacgctcgg ctcaacaaag attttagcca ttatcatcat gctgttattg gcctttacga
    11761 tgtctctttg ctcagaagca gatgcgttta taggatcttc tttgttaagt ctgttcggca
    11821 cagcacctgt agctgccttt ttggtctttg gcccaatggt cgatatcaaa aatctactga
    11881 tgatgaaacg atattttaca ggacgcttta ttttatcgct gatcggactg attgccctta
    11941 gcgttattgg attcacatta tttatttagg agggaaaata tgctgcggtt tttgatttta
    12001 tctgggtatt tcgaattgat gatgtacttg caagtttctg gcaaactcga tcaatatatc
    12061 aatgtccatt atcgctactt agctattctt tcaatgattt tatctgctct tttggcttta
    12121 gttcagctgt atttgtgggt caaaaacgga aaagcagaag aaaaggcaca cgagcacgat
    12181 catgaccatg ggttgtcaaa accttatcaa cgtggaatcg cttatgtact gctagctttg
    12241 ccagtaatcg tggggacgtt gtttccaaca gttagtctcg acactacgat cgtagaagcc
    12301 aaaggattta atttcccagt aagtaaagaa tcagtagggg atccggagat gcagacacaa
    12361 tacttaaagc cggataccag tatttatttc aacaaatcag actacttaga ccaaatgaat
    12421 gagttgaaag aaaagtatgg ggataagcaa actatcaaga tcacagatga aaactattta
    12481 gaagttatgg agctaattta taattaccct agcgaattca ttggcaaaaa aatcagctat
    12541 gaaggatttg tttaccaaac accgaacgat agcaatacag atatcttctt gttccgattc
    12601 ggcattatcc attgtgtggc agattcagga gtctttggtt tgttgacgca tatgccggaa
    12661 ggagtaacgg ttaaaaatga tgaatggtat aaaatagaag gaaccatcca atcagactat
    12721 tatcaaccat tcaagcgtga aatcccatct gtagttgtta caaacgctga gaaagtctct
    12781 gctccgaaaa atcagtatgt gtatcgttct ttttaaaaga acaaaataaa taaacatagc
    12841 tcagacagct gacacgcaag gacgtcggtt gtctggcttt ttttgcatga tgtattcatt
    12901 tgtttgatat ttattttaga agcagcatcc tgaaagtatt tgatatcggt ttcaaaggtg
    12961 ttggaaaaga ttgtgaagag aattacaatt tattcctaac atgtatgttg aaaagttgta
    13021 tgaaatgatt ttgatagtaa aatatcgagt ggaggtactt tttatggtaa cgcgtatatt
    13081 tattttggat gatagtttgg tttttgaaaa aatgctagta gaaattttgg aagaatccag
    13141 aattttatta agtccttgga aatttgagat cgttcaggga ttgaacgtta tacaatttgt
    13201 tgaattcatt aaaaataatg aaatctattc ctcagatatt tttttcctag acatcgattt
    13261 gaacgcctat tatagcggta tcgaactagg cgaaatgctt agagaaaaga atgaaaagtg
    13321 taaaattgtt tatctaacgt ctcatccgaa taaagcaata actgttatta atcgaaagat
    13381 tacgcctagt ggttatttga taaaagaatt cacttatgaa aaaaataaaa aaagcatcca
    13441 aagttactta ttgaaagaaa aagaaaacta tcctgttcaa catgctacga aaaaaaggtg
    13501 gacaactgtc cgttatggta atagcgatca ctatatccat tacgatgaaa tcctttatct
    13561 aatgagcatg ccaggttata aaaataaaat aggcctagta ttgaaagatg aagagattct
    13621 cgtaaacggc acaattaaaa aatataaaaa atcactcgaa tatgagtatt tatgtaaaga
    13681 tttaaaagct tatatcatta atactgaaaa cattacatct attagcagag taaacgaaat
    13741 agtaacattt gcaaatggag aacaactaga gatgggaaag agaagtattg ataaactaaa
    13801 gaattttatg caagaaaaga tagaaaatga atcattttaa agaaaacaaa gactcatatc
    13861 ttacctgtta gaagagtgtt ttcctgtaga agagaacaac ccaagtagca gcctagtcgt
    13921 ttctatcctt ttttgttatg cactcttttt gacatttact ccaccaataa cgaagggtac
    13981 aataaaaagt aataacaaac aaaaggagta cagaaatgat tgaattcatc aatgtaagta
    14041 aaagttacgg gaaaaaacaa gcattaaaag acttgtctct ctcgataaga caaggggaaa
    14101 tttttggatt tttaggacat aatggtgccg gaaaatcaac tacgattaaa agtttagtta
    14161 gtattattga accatcatcg ggaacgattt tagtagatgg aatgaaattg acggaaaatc
    14221 gattgagtat caaacaaaaa atcggttatg tgcctgattc cccagatatt tttttacaat
    14281 tgacggctgg agaatattgg gacttgattt cagctgccta tgaattagac acacaaaaaa
    14341 aagaaaagcg tttagcagaa ttgacagcgc tttttgatat gtatagtcat caaaatgaaa
    14401 caatcgcaag tttttcacat ggaatgcgtc aaaaaacgat tttgatcggc acgttgctgc
    14461 cagatccaga tatttgggta ttggatgaac ctttgcaagg acttgatcct caagcagcat
    14521 ttgatttgaa agaaatgatg aaggctcatg ccgcaaaagg aaaaacagtc attttttcta
    14581 cacatgtatt ggataccgca caacagttat gcgataaact ggctattttg aagaaaggtg
    14641 aattgattta tcagggggca gttagtgaat tattgaatgg ctcacctgat gaaacgctag
    14701 aaaagattta tttgaaaatg gctggaaggc aagcgacagg agatggaccg tatgtataag
    14761 cgacaattat tagaattaat tcgagttaat cttttatatg ttaatccaca gctcacgaat
    14821 aaagcaagag agaaaggaaa aaagggaaag cgattaacta tctatctttt gtctcaaaat
    14881 cttttttcag ggctgatttt tttactgatt tacggatttg ctatgtttgc tatgaacttt
    14941 gttaaattgc caggtttctt tacttattac gtggcacttt ttggtatgtt aggtttaact
    15001 caaagtatat cagtcattta taatatcttt tttgaaggaa atgacctgca agtatttttg
    15061 cctctgcctt ttggacaaga acaggttttt tcagcaaaga ttttggtagt tgctttgacc
    15121 atcattcctt ttgtttttcc gcttctagtc atgtttattt taaccggatg gcgagctgat
    15181 ctagggattt tgcttactat cattatctca atccttttgt tttctctatt tttgattatt
    15241 cttttttgtc tggctagttt gattgttttt ggtttggctc gaacagcagt gtttaaaaaa
    15301 catagaaaaa cagtttctag tatcttgctt ggcagctcga tgctgatcgc atttgctgga
    15361 atcatgtgga tgaatcatca aacggggact attgaaaatg agttaacaga tcggcatccg
    15421 atttcgatat tattgccatt ctatgatgct gccagtcagc cactttctca acaaggttta
    15481 ttgggttggg gaatactagc tatcacggct ctgggatttt tccttgctgt aaaacagctg
    15541 atagtaccaa agcttacaga gcaactgact gattcaaaca gtgagttcaa aataaaaaga
    15601 acccataaaa agaaccaaaa tgtgcatcag cttttattta gttacaatgc tcaattagta
    15661 aaagaaccta atttgattat gcaagttctt tcttcttcct tgttaactcc aatcattttt
    15721 attcttgctt ttgccctagg aggcgaaata aaattaactg atttagataa taagttaatc
    15781 ggtgttgttt tcctggtagg gatcgcattg gctttcttaa cagttaatca gacttctttt
    15841 atcagcaatc ttatttcttt ggatcaagaa aattttctgt ttatccgttc tttgccaatc
    15901 tctatgaacc aatatttaaa agaaaagttc agattcggat ggatattgca gagtttttta
    15961 actggtggga tcgcattatt aagcggactg tcttttcagc tgcctttgtt ttttatccct
    16021 agcttgctat ttggttcttt attcggctgc tacttattgt cactacgtta tttcgctaga
    16081 gattatcggt tgctgctgct gaactggacc aatattaacc aattatttaa cagaggttca
    16141 ggaagtttgg gtttagtcgc aactatgatg ggttcattga tcatcagtat tattcttatc
    16201 gtcttatacg gaatgttggc tttgttttat ccattttggt tagtgaatat tcctgtgatc
    16261 ggtttagttc taatactttc cgctctatgg atcgtttatt atcaacgaac gttttggaaa
    16321 aaatttgaat aaagaagagg ggccgtgaca aaaatcatgt cgcagcccct tttttacaga
    16381 tagagggtgt tcaaaagtca gtttctcagt attaagtcaa ttttatgtaa aaatgtgaga
    16441 agctattttt acaaaacttt ttttaatact cataattact tcttattgct cctgcggtta
    16501 cttgtcgcaa tgtttgtgag atattttgtc atcgctcaag gctgaacgtc tctttcacaa
    16561 ccttttcttt gttacacttc gtttatttaa aaagccgttt ttttcgtttc atcatgattg
    16621 cgtcacgctt tactttgaaa ttacggatgt tatggttaaa gacttctgat aatacaagac
    16681 ctaaagcgat tgctccagca atcataattg cttggaccgc ataatgagcc gcttctcgat
    16741 aggattcagt aacgagattt cgaaccgctt gataagctaa accaccaggg actagaggaa
    16801 ccatcccggg aatattgaaa atcgttacgg gcattttcaa acgtttagag aaaagatcgc
    16861 tgatgaatgc tactcctaaa gaaccaagca atgaaccaaa agcagcagat ccgcctattt
    16921 gaacacagat ccagtagatc atccagccaa aagcaccaga aagaccacaa caaatcaatg
    16981 aacgcctagg tacatttgta ataacggcaa atgccgcagt cgctaaaaaa ctaaaagaaa
    17041 actgaataaa tatcgtcccc atacttgtgt tcctttccta ataaaaaagc tggaaaataa
    17101 aagcaatcgc aaaaccgatc atcgtagcag tcatcatcgc ttctgttccg cgagaaactc
    17161 ctgagacata atgtccagct agcaaatcac ggatagcgtt cgtaatttga actccaggaa
    17221 ctaaaggcat cacacatccg ataatgatta gatcctgatt gcttcctaag ccaaaacgta
    17281 cgctgtagat agcggctatg ccaatcaata aagaagataa aaattccgat aaaaatttga
    17341 ttctaaaaaa tttcaaacta tatagataag ctgcgtatcc caatcctcca atgactaaag
    17401 tcacaggtaa atcggtcatc tttccgccaa ataacaccat aattgttcca ctgatcactg
    17461 ccgcgctaac gatttgcaac cagacaggaa agaaagaacg gtctcggtcg attgcttgca
    17521 gttcgcagta aagttcttct aatgttagct ctgaagcaac atatttacgt gaaagatcat
    17581 ttacttttac tactttttct aaattaatcg aacggttggt aatttgttcc attcggatgg
    17641 tcgaggtacg atcaagacca ataaagagac cggtttgagt gacatagctg accaaacgga
    17701 aatttccaga agctaaagca attcgactca tcgtatcttc tacccgatac atctccgcat
    17761 tgctttccat catgattttc ccagcgagta aacaagtgtc cagcaacaga tctaattctt
    17821 ttcccgtcat agcatcttct ccaataattg attgattagt tttattttac tacaaaacat
    17881 accagaagcg taaagaataa aaaaacttga ggaaggaata aaagaggcgt atactgtttt
    17941 tgtattgcgg gtgtagttta gtggtaaaat cccagcttcc caagctgatg tcgcgggttc
    18001 gattcccgtc acccgcttaa aataggttgt gaaaaagctg ctctgcaaca agtaataaga
    18061 acagccaagc aaaaacaact tcttatgttt ttaacttgga tggacttatt accgcagatg
    18121 caagcttttg cgcaccgttt accaagcggt tgtgaaatca gtgtattgtc ttgagtatga
    18181 agaaaatttt tgaataataa taaatacctt acagatagag tggtgactga ttagagacga
    18241 ggaatccgtc tcttttttgt atttttaagc catcttaaaa caggttttat tttttgctgt
    18301 ttctatttct ttctagtatt tatttatagg ggaacatatg ttaagattta gggagctgtt
    18361 agtaaaggag taaagaaatg agcaaaaaaa caaccaaggc gatcgtgggg atcgtcgctg
    18421 ctgtgattgc aggggcattc ggtgtttcca ccacacaaaa agattcagat atctttcagc
    18481 agattacaac tatttttgat tccacaccac aagcttctca agtagcggat gtgccagaat
    18541 atgatggaaa acatcaagaa atagaaatca atgggaatca gccggaattt acaaaagagg
    18601 atcttagcct ttccaaagga acatgggaaa gctattcaaa tattgataaa ttgaacagag
    18661 taggacaagc caatgctatg cttggaaaag atttgttccc tactgaaaag agagagcctt
    18721 tgtatattga cccgacaggt tggaaacaaa agaaattaag tgacgggcaa tggttatata
    18781 atcgcagcca tttaattggt ttccagctga ctggtcaaaa taacaacatt aaaaacttga
    18841 tgacagggac acgttcgttg aatgctccat acatgcttgc tcacgaaaat gatattgcgg
    18901 catatatcaa agaaacaaat catcatgtac gctaccgtgt cactcctcat tttgagggga
    18961 atgaactggt agccagggga gtgcaattgg aagcagaaag cattgaagac aaaaaaattg
    19021 aattcaatgt ctttatctac aatgttcaag atggctacac gatcaattat gagacaggac
    19081 aagcaacaaa aacaaaaaat tagtagcagc tattcgaatt ttttatgaat attatgaaaa
    19141 aattgtgtaa aaacatacat tcaaagaaat atagcgtata ctttatgaaa agagtacttt
    19201 tgataaagct acagaaaaaa ggaaaaaaac atacaaatag tgtttcttaa tttattcgga
    19261 agtttggaat atttgtttgt agttttccct ttcttcgagt aaaataaggt tgtagaaggt
    19321 aagctggaat taccttgcgg gaaagaggtg tagaatcatg ggttttacag atgaaaccgt
    19381 acgttttggt tttgacgata gtcgcaaaaa agaagtgagc gaaacattag cgattgttta
    19441 tcgggctttg gaagaaaaag gctacaatcc aatcaatcag attgtcggct acttgctttc
    19501 tggagacccg gcctacattc ctcgttatcg tgatgctcga aatttgattc gccgtcacga
    19561 acgcgatgag atcatggaag taattgtcaa ggactacctc tcaaatcatg gagtgaattt
    19621 atgagagtaa tgggcttgga tgtcggttct aagacagtag gtgttgctat cagtgacccc
    19681 tttggctgga cagctcaagg agtagagatc attcggatca atgaagcaga agaagagttt
    19741 ggctttgatc ggttgaagga attagtagca caatatgaag tagaccggtt tgtagttgga
    19801 ttacctaaaa atatgaacaa ttccatcgga ccaagggcag aagcttccat gtcatacgga
    19861 gaaaagatca aagaactatt ctccttgcca gttgattatc aagatgaacg attaaccact
    19921 gttcaggcag aacgtatgct agtagagcag gcaaatactt caagagccaa acgaaaaaaa
    19981 gtcatcgata aattagcagc tgtgatgatc ttacaaaatt atttagacgg ctcaggtaag
    20041 caattatttt aataataaaa gaaaagaggt aatcttatga cagaacataa ccattcccac
    20101 gaccatgaag gacacgaaca catcacattg gtggatgacc aaggaaatga aacattatat
    20161 gaaatcctac taacagttga cggacaagaa gaatttggac gtaactatgt tcttttatat
    20221 cctgctggtg tttcagaaga tgaagatgta gaattacaag cttatgcata catcgaaaac
    20281 gaagatggaa cagaaggcga gttagaacaa atcgaaacag acgcagaatg ggacatgatt
    20341 gaagaagtat tcaatacatt catggcggaa gaagaagaat agactataaa gctaggagaa
    20401 accttgatat gatagggttt ctcctttttt tattttttac tgtatgccat attgtatgcc
    20461 atctagtcaa tatttaagtg ttgtactaat ttttcaacag tcttttcttt cgtttctttt
    20521 gccaagtgac cgtaacaatc gatcgtctga ctaatttttg tatgccgtaa tctagtttga
    20581 acttctacca tagtagctcc agcgtccaag agcattgaag catgagtgtg tcttgctaca
    20641 tgcacaccga gaaatggaac catagctttt tcacaaatag taatcaatct tgcataaata
    20701 gcgtggtgtg tgattagttc tttatagata ccacaaaaaa tcatagtcgg atcagttaca
    20761 ccaagtgtca gcatatattt ccgctgatta atctgccatt tttttaatat agaaattgtt
    20821 ctttcatcta gtggaagaac cccagcacta caatcagttt taggatcact gattttatag
    20881 ccatttttgg attggcttag tgttttattg atggtgaccg tattttcttg aaaatcaatg
    20941 tctgaccaat tcaatgcaag ggcttcactg atcctaaccc cgctataaaa gagaaagcgt
    21001 agtaacgttt tatcaaagtc gtttcgataa gtttcaggtt tgctgtttag gtaatcaaac
    21061 aaggtgatca gttgttcttt actgtaaact ttgacctttt ctttatgctt agtggtggtt
    21121 cttttaggca ttaatacgtt gtctagtgga ttagtctcaa taatcccaag attgatagcg
    21181 tagctaaaaa tacgcttaga agttgaatga agtaccttgt attggtcagt agtttcagcc
    21241 cagctattta caatttgttg ggcttttttt accgtgactt cattcacacg aagatttcca
    21301 tattttggaa gaatccactt tctaaacttg atttcagttt tcatgaaggt ggcttctcgg
    21361 acagtggttt tatatgtttc tagccacaag tcataaactt ctttaaacgt ttcgttgttc
    21421 gcttttttaa taccgttttt atcgtagtcc acttgcaagc gtgacaaggc aagctgggct
    21481 tctttttttg ttttgtaacc tcgtctacga accttatcag gtttgccagt cattttgttc
    21541 attccaagat aggcttcata gagccaaaat ttaccttttt tagtttcata ttgcttgaat
    21601 gttgccataa tagttttccc tccatagtgt agcaagccgt gggggcggtt ggaggaggca
    21661 gtttattatt attttgaata gtgtttattt agctcagtaa caagagctgt gaactctttt
    21721 agaagatcat tttcataaaa ttcttgatac tcttttttct catctgtatt tttttctgaa
    21781 tcattaatta atgagtcata tcgtaaaagc gtatttagaa gagctgcgag ataagcaatt
    21841 ggggtactcc cgtcaggaaa ttcaattcga gatcctgttt taagatattt tattgcgttt
    21901 gttaaataaa tcttctgtga gacagtcagg ttatcttctt cgatgttatt gatagcatta
    21961 gaaagattag aatcaattat agagttattt ttttcaatag tttttttcaa ttgcttttca
    22021 agcacatttc gcttaatttc gggcatcata tgcatatctc ttaatgtata agatccgttt
    22081 aatagatagg tagtggatac atctaatacc tttgaaagtt gtttaagcct ggtgttattg
    22141 ggaagattat agccattttc ccaattacta acggcacctt tactggccgg tggggataat
    22201 ttacctccaa gttgttccat tgagagtccc ttttcaattc tagccagtct aattctttgt
    22261 cctgtctcca ctgcttcggg gtattttttt ctagccacat tttcaccacc attcatatat
    22321 tttctctact atatcacgat ttaaatatgt gtgaaacata aaagttcaaa aaagtatgtt
    22381 aaaattcttg caaatgaaaa ggtatgatgg tatattgagt ttataagagg tatagaaatg
    22441 tattcaagag cctcttgcat ttttttaaaa tctttgtata gaaatgtatg ctaacgaggg
    22501 gggataaata tggaattaaa aattgacaaa ctcacgcttg atgatccagc ttttaaaatt
    22561 attgacgatc gagttgttca acaagtagag caaactcaag gaacgttact ttcattatta
    22621 gaaaaaaagt accagtttcc aaaatatatg aacaaggcac aagctgctaa gtatatgaac
    22681 gtttcataca atactatgat gcagaaatat gttcctaacg gtttaaagtt gattatagtt
    22741 gatggagtcg taagaatttc acaagatgaa tgtgatcgct ttatggaaga ataccaaaaa
    22801 taaacgcaag ccgtgggggc aaagggatta ataggaggaa ataataatgg aatcgacaca
    22861 aaataaagca atcgaaaaag tattatcaac aattcttaca gaagacacag cggcagaatt
    22921 agcaaacttg gaaggcaaag cgctagaaga aacgtttgaa tgtttgtacg aacaaatgga
    22981 ctatcaaaaa ttgctaccac aagaaccaac ggtaagcgga gtattgcgtg gcttgaacga
    23041 tttagtacaa gcagagttta aagaacgact ttctattgaa gagtatcaag aaattttgta
    23101 tcagcaagtt gagttgttag cgagcttgct agggcttgaa ctggaggacg ttgaatgaaa
    23161 actgaaattt ggaacggaca cattataaga tttgtcgata ttaatgatga atggtgggca
    23221 gtggcgaaag atgttgcgga agcgttaggt cttaaacagg ttactagagc cattcactct
    23281 ttgcctaaag atggggttac tactagtaag gtcatcgatt cattgggcag aacacaagat
    23341 gtaaacatca tcaacgaaaa aaaatattta ccgcatggca ttcaaaagcc gtaaaaaaga
    23401 agccgaggca tttcaagact ggattttcga catcatcaaa gaactacgcc agtccacagg
    23461 acttgaaggc tttcaagtat tccgcatgct agacaaagaa caccaaaaag aagcaatgac
    23521 taaattaagc cacgctatta ctgaaccaaa accagtagac tacattaaag cgaatgtgat
    23581 agccaataaa gctgtatcaa ctatttatgg tcattccaaa atggtaaaaa agaaagatat
    23641 gactcctgaa atgttggttg atcgtgaacc tattttagat gagacagtag aattaatgac
    23701 tgttaaagaa aaatatgggt tgcagttttc agtgagtgag aaaatttata atcgttctgc
    23761 tgaattgcag acaacttagg agcattaaca tgatcgagaa caaagaaaaa gccttatcca
    23821 taacagtctt ggcggacagg ataaaggcaa cttaattggg ataaaacccg atattctttg
    23881 ttccgattat accatattaa aaaatcggag ggaaccatta tgaaacgaaa cttaattata
    23941 ggaagcattt taatcggcgg aattttcggc ttattaccat taattgcaaa aatagctatc
    24001 tttgcaggat tagcgttata tctcttcact gcttatgacg agtacgacta tcaattaaga
    24061 actggagggc ataagtagat gtacaaaaca aaactattaa accaactcga tagccttgaa
    24121 ttagaggaaa ttaatcaagg gatcgctgaa ctagaaaata atatcgggaa aacctacttt
    24181 ggaaattcat tcaatgaaaa actaacagtt ttgtatgtac tcaaaaaaca tgcagaacac
    24241 aaaataattt gtcgagaaat caacgagcta aaaaatcaga ttttaaccgc atggttaaac
    24301 attactgata tgcaagaagc aagggtgaaa acatttaata catgggtaaa gtatcagaat
    24361 caattaaaag gtgctgaatt tgttcgggat gggttgaaat acgaacttga gcagttgaag
    24421 ctcatggagg tgtctgaatg atttatacca gtattttgaa aaaggaggag aggtttattg
    24481 gcaaataatt atcaagcagc aacggaatta caaaagtcgg gatggtcaat ttatccctta
    24541 tctcctggaa ctaaaattcc aataaaaggc tctagtggtt ggaatgatgc aactaacgat
    24601 tctaatcaaa ttaatgaatg gtggacggaa gatgcaacta gaaatattgg gttaatgctt
    24661 gaagatcatc aaatggtggt agttgaccta gatcaacatt ctgaaacacg aaacggcgtt
    24721 gagaattata aaaaacttgc ttcaatgtat gatccatttc cttctactta tacggaaatc
    24781 acaccaggta aaggaatcca tttcttcttt aaaattcctg atggtgtaat tatccaacaa
    24841 gaaacaggtg catttgctga tatcttcggg cgtgatgata aaggtaaaag tttaagcggg
    24901 attgatatta tcactaaagg agttccaata gcgccaacta tgattatttc aagaagtgtt
    24961 gggagagcat atgaacctat tgaaaacgta tctctaaata atattgcacc agctcccgat
    25021 tggctagtga acctactgct aaaaaaaccg caagaaataa agactcaata ccgacccgta
    25081 gcaaaaacag ggacagcaaa aaaattagat atgattgttc aaggcgctgg cgaaggtagt
    25141 agaaacgatc atttaactaa gttatgcggt tggttactat ggcacggtgt agataatcaa
    25201 actttagcag aattgattta tacagcaaat gcgtacaatg aaccaccgct aaaagataaa
    25261 gaagtgaatc aaattattcg atcgatgatt aaaaaagatt tgagggggaa agctcatggc
    25321 tcataagcat aaattagata gtattttaga ctttcccgaa gccagcgagc gtgaagacaa
    25381 tatcattgaa ttgaaaacct ggatgtcacg attaagatgc aataaagacg atcagattaa
    25441 aagtaacagt gttgtgaatg cagaattgat tttaaccaat gatagcaatt tagctggaac
    25501 aattgcttat aacgaattta gcggatacat tcacctatta aaagattctc cttggatcaa
    25561 tcgtaccgct ggagaatggg aagacagttt tgaggatgcc ttaacggcat atattgaaga
    25621 aaactacaat gtagtttttg atgacaacaa aatacacaaa gcagtagtaa atgtggctag
    25681 aaagaatgtt ttcaacccag taaaagaacg tatcgaaaaa gttaaatggg atcaacagcc
    25741 tagactggaa acactgttta ttgacctttt aggggtagac gacaaccttt acacacgaga
    25801 agtaacaaaa cgttggatag tcggatcagt cgcacgtatc tataaaccag gaataaagtt
    25861 tgagatcgtt ccagttttgg atggaccgca agggattggt aagagtaccg ttccagcatt
    25921 actttacacg gatgattttt tcaccgattc attagattcg ctaggtgaaa agaaagatga
    25981 ttatatgcag cttcaaggca atgtaattat tgaacttgga gaattatctt caatgaacaa
    26041 aactaaaatt gaacagataa aaaacttcat cagtgccaag atcgataaaa ttagacctcc
    26101 ttatggtcgg aatgtcatag cttgggcaag gcaatgtgta tttattggaa cgtcgaatga
    26161 tgggcagtat ttgaaagatg atacaggaaa ccgccgtttt tatcctttgc cttgtaagaa
    26221 taagccaaag atgaatccgt ttaaaacaaa ggatgaatat tttcttcaag tgttagctga
    26281 agcaaaggtg ctttttgata aaggacaacg aatctatttt agtcccgatg aagataaaga
    26341 agtcttagaa attgcaaaag actatcaaga ggatgccaag atggagaatc caattaaaga
    26401 agcaatcact aaatatttgg aaatggaaat cccgtataaa tgggaagaag ctccagcgtg
    26461 ggcgagacgt tcttattatc agaactatcc tgacagtaaa tgtaatgaga aaaatttaca
    26521 gcatttctat aatggagcta ctatgcaaca agagttttat ttagttgata gcgtactcac
    26581 cgctgatatt ttggaagctg tttttgataa acaagcaaag gatttattga atggtcgatc
    26641 agatgcagaa actaaaaaga ttgccttgat tattagcagt attcctggtt gggaacggaa
    26701 gcaactgcca aaaagaaata aacgacgagg attttacaat aagtcaaatg cctatgaaaa
    26761 taaaaatcga gctggaaagt acaaaaagta acgggtagta acgggtcagt aacacctagc
    26821 ccgttactag tttaccctta ctgccccaag ggatacagca atttggtaac ggggtaacac
    26881 cttatttcct taaaactttt gaaatagtta gctaatatgc tggggaataa tgttactgga
    26941 ttttacccgt tacctgttac caaaacgcta gaacccttgt ccccgttggg attgagtggt
    27001 aacgggtacc tgttacgtag gtgttaccac gtgttacgaa tcaaggagga aagtaaatgc
    27061 tcaatattat ttcaacaaac aaagcaccga actttcaata tacagatgaa atggatcggt
    27121 ttctaatgaa cacgctggca tttagtgtgg gattggtaac agaagattat tccacatttg
    27181 atccagaagt attaaagata atggaagaag aacctgattg gctgcaggag tcagtagcgt
    27241 ggtgtcaatc gctagtagtg ggatcattgg tagatagtgg caactatgat gatacaggtg
    27301 agctaatgga tgaatttaat tgtctgctta acctatatga tcgagcgaga caacgagagc
    27361 ttacatcgaa tgaggataat ttgtttttaa acatccatga taagttcttg gctttgctac
    27421 taacagatga tgaattgata actaatttat tggaggtaga ataattatgc atatggaaag
    27481 tattagatac ttcgatggtg agaaacacat tgagtattca aacactcaag aagatgtaga
    27541 ctttattagt tttgaggaag ataaaatcac taaagttaat tttaaagacg gaacgtgctt
    27601 gaagatagtt tcgccttata ttgaatacaa atcaaaatgg gaacaagatg attctgacga
    27661 acgagatcgc tttggttggt aatctatggc agttaaaaaa caatgtaatc atgctggatg
    27721 taaaacattg attgattata ggcagaagta ttgtgggaag cataaagcaa agcaaacggc
    27781 aattaagcgt gaagaaagaa agcaaagcga aggtaaatat tttcaattct atcaaagtag
    27841 aacatggcga aaggcttcat acttgtatcg gttaaatcat ccagtatgtg aggattgcct
    27901 agaagaaggc ttgataagga aagctgatgt tgtagatcac aaaattgaac taaaagatga
    27961 ttggaataga cggttagatg aaactaattt tcgttcttta tgccatgcac accataattc
    28021 caagacagcc aatgaaagac aaagacgaga aaagagtacc ctttgagtta agggggcata
    28081 gtgtgaaact tactgacaat cgatgcctac tcattttggt acaaataacc gttgtgaaaa
    28141 ggcataaggg taattacaaa taacgattac acttgtaatc acaagtgaaa aagtatataa
    28201 tgaaagtagg agatttattg aaaaatacaa ttgttattac tgataaaaca gggaaacagc
    28261 gcaccattga gttacctcat tttggtcaag tgaccattca aatgcagaac ggaaaaatca
    28321 tttatataga taaattagat aaagaaaaat tctgatcgaa aaacgaagga atgtgagctt
    28381 aattgcttac gttccttttt cttttgtctg aaaggaggac aacatgggac accctaaaat
    28441 attacacgat acaaaaggca atatatcaag tgaagaaaaa gcaatccgtg tggacgctag
    28501 agaagaattg ttcaagcagc aaccattaat aaatatcacg ccccctgact ggatggcagc
    28561 gagcgctaga agtgaatgga atcgtatcgt accaacatta aaaaaagatt atccattgag
    28621 tgaagcagat tatggttcat tggtagccta ttgtttagcc tttgctcgaa tgaaaacagc
    28681 cgaagccgag ataagaaaat caggtacgtt tatcacatgc gaaaacggag taaagaaagc
    28741 caatccagcg gttcgggttc aatcacaagc tatgagtgat ttgaaaaaac aagccacctc
    28801 actaggtatg accttagaat cacgatcaaa actagctttg aacaaggtta aaaatgatga
    28861 acccgaagac ccgttcaaag agttgatgaa ttcatgaatg attacattga gaaagtccta
    28921 tcaggtaagc tgattgcacc caagaaaatt atccaagcgt gtgagcgtca tataagcgat
    28981 ttggagcgtt ctaagtcaga tagcttccct tatgtgttcg atgaagaaca agccaccaaa
    29041 gcgattaaat tcattgagtt gctaccatct acggacggta aagcaatcaa gatgttagga
    29101 tttcaaaaat tcattctagg cagtctttat ggctggcgta ctaaagaagg gaattacagg
    29161 cgattcaatc gagcgtttac cagtatgagc cgtaagaatg ggaaaacgta tatcgcaagt
    29221 ggcatggctg ccaatgcgtt gattatggaa caagaaccag cagaagcaag gcaagtattg
    29281 tttgtaagta acgctttgaa acaagctaaa ttgggctatg atatgctgtc taattcactt
    29341 agaaacgtgg tcaagtctag taagttttta agaccacaac tgaaaattat gaactctaag
    29401 attcagcact tgccctctaa ttcgttcgct atggcactgg ctagtgaaac cagcacgcta
    29461 gatgggtttg caccaacaac cgcaattctt gacgagtggc acgaagcaaa aactcgtaaa
    29521 acgtacaacg tcattaagtc aggaatgacc caacaaaaga acggcttatt gtgtgttatt
    29581 agtaccgctg ggcttgattt aaacgttcct atgtacgagg aatacttatt gttagatcgt
    29641 gtgctaaaag gcgaagaaca agccgacagg tactttatag cgatatggga attggacgat
    29701 cccgaagaaa ttcacgatca agagaaatgg atcaaagcca atccgatttt tgaaagtgaa
    29761 gaaatcaaaa aagtaatgat tccaaccatt caagatgatg tgaaccttgc tttgaaacaa
    29821 aataacctta attctgtatt ggtgaaaaac ttcaatttat ggagacaagc gagtgaggac
    29881 agctatatga ttgctgaaga ctggcaggca accgaagtag aaccacaaga tattacaggc
    29941 aagcccgttt atatcggagt ggatttatct aaaacagatg atttaactag cgtttcatgg
    30001 atcgtaccgc tagataacgg cgaactttat tgtgattcac atagctttgt agccaccaaa
    30061 tatgggcttc aagacaaaga aaagcgcgac ggtttgcctt atcgagaact agaaaaagct
    30121 ggtgagtgtt ccattactca attagaaagt ggaatcgtgg actatgacca agtatttcag
    30181 tttattcaag atttgattca agaaaatgat ttggaatgta tggggatttg ttatgacccg
    30241 tataacgcta attcgcttat cagtaaagct gaaaaagcca actacccaat gttagaagta
    30301 agacaaggaa cgattactct aaacgttccg acccgaactt ttagagaaca agtttatgaa
    30361 ggcaacgtta ttcacaataa aaatacgatt ctcacccatg cagtgaacaa cgctatttta
    30421 aaaacggata acaacggcat tcagattaat aaatcaaaga acagtaacaa aattgatcca
    30481 atagctgcat taatcaatgc ctatgtgttt gcaatggatt acttcaccac aacggaagga
    30541 gcgaaagcag acaatgaatt ttatacaagt gaagaatttt ctttctaatt acattcatac
    30601 cgttctttta cttcttggat tggtgtgtgt gttggttgct atcaccttac taacaaatgt
    30661 ctactatggc ttgttagcgc tgggcatagt gcttattggg gtagcggtca tgctaaatac
    30721 agaacagaag ggaggttaaa agatggcatt ttttaaagcg agacaaaata caacgggaga
    30781 tcctttccta caacatgtgg tatcaatcca atcggatgat tacaccacca gctttacaag
    30841 cgttcgtgca ttaagaaata gtgatgtgtt tgcagccgtt cgaattattg ctagtgatat
    30901 tgcttcaagt ccgattcaat tggtcaaaaa caatatgcca caagctgata atgaactggt
    30961 gaagttacta aacgagaaac ctaattcaga aatggatggt tggcatttaa agtttgcttt
    31021 ggcggtcaat atgttgctaa acggtaatag ctttgcagag attaaacgca acggtgaaaa
    31081 ggtagaagaa attcacttat tacctaactc aagtgtaacg gttactcaat tagataatgg
    31141 tacgttgtct tatcagattg gtgataaaaa aagacgtgtg aagtctagcg atattttgca
    31201 cttcaaatat ttcacccaag atggtttaac aggattacca ccactttatg cgttacgtga
    31261 tgaactgaaa attcaacaag ctggcaaccg cacattgcac aatttcttta ctcgtggtgt
    31321 cagtgggtca ggtattttga aggttcataa gtcagattta gacggatcag caaagaacgc
    31381 aattcgtgaa aagtttgaag aagccaacgg ttcaagtagt ggagataatg ctcttagaac
    31441 gatcattctt gatgaaacaa tggactacaa aacattagaa ataaacaccg atgttttgaa
    31501 gcttatcaat tccaatgatt ggaacacaaa acaaattgcc aaggcgtttg gtgtaccgat
    31561 tgagcgttta ggcgttgaaa atgaacattc tagcaccgtt caaagtaatc tccagtatat
    31621 ccaaagtacg ctgattcatt actttaatgt gtttgtgagt gaattagata cgaaacttga
    31681 aaccaatatc cgctttaatt ccgatcagtt actagagact gatcctgaaa caaaagtaaa
    31741 aaatgtattg gatcaggtca aagggtcact tctcacgatt aatgaggggc ggtcgaaaat
    31801 ggggctaccc cccatggatg gtggcgatcg tttactagca agtttgaact ttacgtattt
    31861 agatacgttg gagaaatatc aattaaaaga acaggaagga gttacaccag ttgaataatg
    31921 aagaagaaaa ggaaaaacgg ctgacagaag aagctgagct aacagccgat tctcccaaag
    31981 tagagaaaga aaatgaaaaa caaccaacag acggcaaaac tatttcaggc tatgcgttga
    32041 aattcgggca accgtcaaaa gatttaggcg gctttgtaga agtgattaca cccgaagcat
    32101 taaaagaggt ggatttatca aatgtgttct tattatatgg acacgattat tcaaagccac
    32161 tagctagtgt caaagcaggt acgttgaaat tgaatgtcga tgatacagga ttacattttg
    32221 aagcagagct aaccgacacg acctattcaa atgatgtgta tgagaatatc tcaaaagggg
    32281 ttgttgattc tatgtccttt gggttcgtgc ttggattaga ttcattcgac aaaaaagaag
    32341 atggcacaat tgaacgatca atagataaaa tcaaagcact taatgaaatt agtgtcgtga
    32401 ccgttcccgc ttatgattca tcaaatgtcc aagtaaataa gcgttcttac gaatcgttta
    32461 tgagtaacaa ccaagcaaaa caaacaaaca atagcttaga atccacttct aaagcacaaa
    32521 aggagagtaa taacatggaa aaaactttaa tcgataacga aaaaacagaa ttgcgtgggt
    32581 atgaagaata tatccgttca caaggcgaag tgcgtgatgg agtcactact gtaaatgcag
    32641 cggcagttgt tcccgaagaa gtaatcggtg aagtctttga tttgaaacgt tcaaattata
    32701 acttagctca atatgcaaca gtaaaaacag tatcaaatgg acaaggtaaa tatccagtag
    32761 caactaacca acaagcagtg ttagcaacaa aagctgaact tgctgaaatt ggtgatattg
    32821 acgctgaaat gtttacttca gttgattata aagtagaaac tcgtgctggt aagattgcct
    32881 tatcaaatga ggttgtggaa gattcagcag tgaatattgt acaagaggtc aaagatcagt
    32941 tagcgaaatt ggtagaaaac actgacaata agcatatcat ggatttatta aaaacattca
    33001 ctaagaaaac ggctgctacg ttggacgatt tgaaacaact atacaatgtg gcattagacc
    33061 cagcattaaa taaaatggta attctaaacc aaagcggata ttaccaccta gatacattga
    33121 aagattcaga tggacgttac attttacaac ccgatgtgac agcacctagt ggtaaatcat
    33181 tattcggtat gccagtagta ttgattgcag atacattgtt tgccaatcct aaagctggaa
    33241 cattccctat gattatgggg gatattgcac aatctatctt tgttgctcgt agaaatcaag
    33301 taacgactca atgggaaaaa ttcgattact actcacaagg acttgcagtg atcgttcgca
    33361 acgattataa gaaaattgat caaaatgctt cagtgtatat tgagtttacg ccagttgtaa
    33421 caccaaaagt atagaaaaat attgggcggc ggtttatccc actgcctttt tttcattagg
    33481 agagataaaa tggtaatttt ggatagcata aaaaaaagta tgcggatcga tcacactatt
    33541 gatgatgact ttattcaaca attgattgat acagcaggcg aatatataaa aagtgctatt
    33601 gatagtagcg caacggataa agatatggat aattatcagc aatttgattt ggcggtgtca
    33661 ttacttactc aacattggta tttgaaccgt caagaagcca gcagcgaacg gataccagta
    33721 acggtacaag cattagtaca acaaatgaga ggtgcttatt atgccaatca ttaagaatgt
    33781 aaatgaattg actgagagag tcaagtttaa aaagacaaaa cgggtaaaag atgaagatgg
    33841 gcagatggtg gatagtgaag aaactgtatt tgaatgttgg gctaatgtgc gttcacaaat
    33901 gctaaaagac gtgcttgcta gtgtaggtac cattcttgaa ggaacgttga cgtttattat
    33961 tcgatacgat caagattatg aactaacaaa cgatatgaaa gtagcttgga aaaacaagag
    34021 ttataaaatt atttcaatca atgaaggaac ggcgtttaaa gattatacaa cgatcatagc
    34081 taaacagatt tcttagaatg attacacttg taaacgttgt gggtatttag tataattaag
    34141 gtagtaaatg aagggttagc tgcctgaatt ttgcaagact tagctagtcg aaatctattg
    34201 ttggactgaa aattgtaggt gtggttgcaa acatgctgga ctaagaaaga tgatagatga
    34261 tgaacgttca attttaatac aggcatgcta ggactgattg gatggttttg gcaagctgat
    34321 tgtgtagaaa tcaaagaaca cgtctgttgt ggacgtgttt tttttgttat aattcaaaag
    34381 aggtgattta atggacaaag cagagtttac aaatttttat tggaagtatt atctacattt
    34441 agaaaatgag tttattaata ccacagattt tgtgatgctc gatgagaata atttcaaaac
    34501 attctctatt gaatatcaaa aactactact agcgattggt tcggagtgtg agattatttt
    34561 caaagaactt tgcggatttg atagcagtag caataaaagc ataactgatt ttaaagctgt
    34621 tattaaatct agcgaattaa tgtgtttgga taatggtgtt agggttatga attctttaac
    34681 attgacttca ctaaaacctt ttggagatgg gtggcctgaa gatacaccag attggtggaa
    34741 aatatataac agagtaaaac atggtcgttc ttcaaattat aaggaagcaa atttggaaaa
    34801 tgtgttgtat gctttagcaa gtctatattt attagaggaa tatttgcata agaaagtaat
    34861 actagaaggt gaagtagact ttataagtcc agagtcaaat ctatttacgt tatcttggga
    34921 aagaaaacat tcgtcgatgc aaaaagtgac gtttgaaaaa gtggattcaa attatgtagc
    34981 tccactcatt gattttaaag aacaagaaga aatttaaaaa cccccacggc ttattgaagt
    35041 ttttgtatgc catattgtat gccatttggt ttaaatcgct tgaaaaatag tgatagataa
    35101 ctctaattca tttagtcata aacgctgttt tgataacctt tatagttctt tgatatgggt
    35161 ttctcattca tggcggaaga agaagaatag aacaaaagaa tgaattaata agatataaaa
    35221 aaagggattg tgacaaacgt cttgtcacaa tccctttttc caaataaacg atgttcaatc
    35281 agctgcccct cggcattcgc tcgaaaaacg gcgaaatatg aagaactatt ttgccatttg
    35341 tttctctaat aatcaggtca aaacgttgac ttcacagtct cttccttaaa cggtgttctc
    35401 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa
    35461 attgttcctt gtgactcgga gctgaacggc aggctcacga cctctttctt aaacggtgtt
    35521 caaaagcttg catctgcggt aataagttca accaaacgaa aattatgagg agctattttt
    35581 gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctt ttcgtttagc
    35641 actaaatttc cttaaaaagt agaaaagctt tcgaagcctc gtttgtttct tttactgtat
    35701 aataaaagaa aagagtaaca atcgctaaaa gatataaaca agagaaagga aatacaagat
    35761 gattggtctg atttattttg tggtgatcgt tctggcgaac acagttggcg ctatttccgg
    35821 gatgggtgga ggcgtgttga tcaaaccgat tcttgatctt atcggcgccc attctgttgc
    35881 agggatttct ttttactcaa cagttgcggt attcacgatg tccattgttt cgactgtgcg
    35941 acaagtatca agcgggaaat cactgaattg gcagattgtc ggttgggtat ctagtggagc
    36001 tgttgttgga ggagtagtcg gcaacattgt attcgaagtt tttttacagt tatttgaaaa
    36061 cgagaagcat gtgcagctga tccaaatttt tttgacggta ttgaccttgg tttttgcgtt
    36121 cttctacacc aagcatcatc agccaaaatt tcatttgacg tcatggactt ggtaccttat
    36181 atgtgggggg ggttctgggc tttttagcta gttttttagg tattggtggt ggaccagtca
    36241 atgtttcttt gttgatgctg atgtttgcat tgccaataaa agaagcaaca ctatattctc
    36301 tatctacgat tttcttttct caactggcaa aattggtaac gattgcttta acaagtagct
    36361 ttatgcgctt tgatttatct atgttgtttt atgttatccc cgctgctgtt attggaggtt
    36421 tgtggggagc aagatttagc agggttttgt ctccaaaaaa agtgaccttt atttttcaag
    36481 taattgtcct cgtggttctt ttggtcaatt gttacaatgc ctttgtcatc ttacaaacat
    36541 taggagaatc tagactaacg caagtcaaaa tcactgaaac atattgttct aaatgaaaaa
    36601 aagagtatac tatgataaaa aaattcattt taaaaaatca gaaattggct ggtgatttgg
    36661 atgttacggg aaatgcgttt agaagatgca gaagcactac gtgaaatcaa tgcggatcaa
    36721 ttagggtatg atataccatt aaccgttacg gtgagacaaa tgaagaaact gctgaaggag
    36781 ccagagaaaa attttttctt ggtttatgaa gatcctactt ctaaaaaagc agctggctat
    36841 gtacaagcgg agtcctatga atcgattttt agcgatccaa tgttcaatat tatggcactt
    36901 gctgtgtcta aatatgctga aaaaagaggc atcggaaagg ctttgatgac tggcgtagaa
    36961 gaagaagcga aaaaacgagg aataacgaca ataaggctta attctgcaga atacagagta
    37021 gaagcacacc aattttatga acacatcggt tatcattccg ataaaatgca aaaaagattt
    37081 ttgaaaatcc tgtaaatctt tatcaaaaaa atacccgatc accaagccga tacttgatga
    37141 tcaggtattt ttgttttttt gactaagaaa gtaattgcat caagaaacca gagatgatca
    37201 aaacgatggc accaccaagt cgattgccca tttgagcaaa agcaataagt tccatccgat
    37261 tagctgcaga taagacggct acatttcctg ttccgcccat cgagttgttg cataagccag
    37321 cagtaatagt tgattcaatt ggatataaac caaacaaacg accgacaaat cctgaaacaa
    37381 ctgaaatcgc gataacactt gtcagacata agacaacaaa ttgccatgtc aaagcagaag
    37441 ctaatacatt cagattcagc aacgcaatac cgataccggc aagaacagct gctgtcaagt
    37501 ttttgacaat caagttattg aacatgatag ctgcatcttc ataatactct ggtaataggt
    37561 tggcgatttt acagatgaca acgataataa tcataaatgc gtaagcatgg actttaggaa
    37621 caaaatagtt accaatttgt cctaagatga agaaagtcaa tgataccatc aaaccaacac
    37681 ctaactgtgg aatggaaggt ttcacagttc gaggttttcc atcgccaaga tcgccttttt
    37741 ccattaattt accattgccg ttgaattttg gcattgattg tccagcacga gcaactaatg
    37801 ctgcaccgat gatagctaag acatttgtca ttgctgtcgc aggaatcagg cgggaaatga
    37861 tagaagaagc atccgtacct aaaacatttg cataaatcgt tgataaagga acggctccgg
    37921 ctccaacacc accagccatc gcaggtaatg agatgtataa aacggaattt gcaaaaccat
    37981 ttccgataag catgcccacg agtcctacgg caaaaaagga gacgaccatt gaaataagtg
    38041 caacgggaat aaaacgaaca gaagctttca tcaatagatt acggttcata ccaaggatgc
    38101 taccagtaat caaggcagca atataaaagt cccaaaagcc catattgctg acaaagtttt
    38161 ctgtggattt cactacatat tcaggtaaga tcccaaatgt agcaatcgca gcagaagcga
    38221 agatcgcaaa aacggaacca ccacccaaat aacttttgac gatagggatt tttgtcccga
    38281 taaaatgaaa taaattaccg aaaataacca aaactgcgat tggtccaatc atattcgtcg
    38341 gtaatttccc taaagccatt gcaatgatca ggacaaggac catgatcaaa taaagtggca
    38401 aacttacgcc ggtgattttt gtggaccaaa aaccttcttt tttcttttcc tcaactgcag
    38461 ttgcagttgt tgttttttgt gccattttta cagatccttt cttattctgt atcatatgaa
    38521 aaaagccaaa gaaaagcgac aaaagagcag gagttggctg gccgctgttt tgccgcagtc
    38581 gcttttttct ttttagacgt catacaaagt ttctgtatgt tcttttacta taacgatctt
    38641 aatcttctaa agatttatat tcaggaaccc atttagcaga ttcaactgct tctttgatat
    38701 ccgtgatttc ttcacgattc aagttttgtg cgacaactga ttttgcgaca gtttcagcaa
    38761 tcgtttgaga aaattcagtg atttttgcta ctggaggcaa gatagctgct cctggttttg
    38821 ttacatcaac gattccgcct aatgctcgac tagcttgaga aatgatttca gcatttactc
    38881 gtgtagctgt tgaagcaatc aaaccaagtc ctaaacctgg gtacatcaaa gcattatttg
    38941 cttgaccgat ttggtaagtt acaccattat attcaacatc agcagcaggg atacctgttc
    39001 caactaatgc ttttccgtcc gtccattcta ttaaatcttt tgcttttgct tctgccaatt
    39061 ttgttggatt agacaaaggc atgatgatcg gacgtggtgt atgagcagcc atttctttca
    39121 cgatttcttc agtgaatgct ccaggttggg tagaagtacc aatcatgatt gtcggatgga
    39181 tcgcttttac aactgcttct aagtttgtca attcatcagc attagtgaaa tctgagcgtt
    39241 tgcgagcgaa tgggatttga ccaggagtca atccttctgt atcttcaaac aataaacctt
    39301 gtttatctac ttgatagaaa tgttggcgtg cttcttcttc aggtacacct tgacggatca
    39361 tctcatctaa aaggatgttt gcgataccca caccagcagt accagcacca aatgtcaaaa
    39421 ttatttgatc tttcaattgt tcaccggata tattcaatcc gcctaataca cctgctagga
    39481 caacaattcc tgtaccttgg atatcgtcgt tgaaagtagt gatttgatct tcgtattttt
    39541 ctaagatcgc tgcagcattt cctcgtccaa aatcttccca atgcaataac aattctggga
    39601 ataattcggt agctgattga acgaattgat cgatgaattc atagtaagtt tcgccttcta
    39661 cacgagcatg acgattgcct aagtataagg gattattcaa taattcttca ttattagtac
    39721 cagcgtcgat cgatacaggc aaaacttgtg ctggattaat accagcagca gcagtataga
    39781 ccatcaattt gccgatagcg atgtcaacac cgttcacacc ccagtcgccc atacctaaga
    39841 ttccttctgc atcagtcaca acaatcaaac gaatgtctcg tccatctgcc gcgttcttca
    39901 aacttgcttt gatgttttct ggttcgtcga ttgaaagaaa tgcagcatct tgcggtttca
    39961 agaaaatttc gttgtattgt tcgattgaat ccgcaacgac aggatcataa acgacaggca
    40021 tgaattcaac taaatgttcg cccatcaatt tataaaacaa tgtccgattc gtattgaata
    40081 gattcattaa gaagatacgt ttttccaaat ctgtttgttt gcttaagtat tgaccataag
    40141 cttgaacaga ttgttgttcc agtgtttgga cagtacttgg aagcatacca gtgattccat
    40201 attttgcccg ttcttctttt gtaaaagctg ttcctttatt tagaaaagga ttatttaata
    40261 agtttaaccc tgtaagcatg aatgattcct cctaagtttt aatacaaacg aagtatagaa
    40321 gtcagatgat gatatagtca aaaagagcag gtatgataaa ttttatttat atgtaatata
    40381 gccaaaagaa atcaaaatag cagtatttgt tctttttata tcgacaatcc ttgcttgaat
    40441 actatgcgta tctcttatat gctagagaaa gaaaagtaga agaataaggg atagatggaa
    40501 tggataaata ggatttctga ggaaaaaagc acatgatagc gttgatagga gtcacctatg
    40561 tcaggtgata aaaatacata agtagaaaat taagaggagg aagagaaaat gaaagaagtt
    40621 caattgatta gaaaaagcga actatcagaa ggcggttgca atgcatgtgg cgtagtagaa
    40681 gcgacaagct atacattaaa attagattcc aatcaagcaa taatctctga actgacagta
    40741 ggcggtcttg tggacagtct ggcgcttgct gaaggtttta ctggagaaga tatttatgag
    40801 atgttttcag aagtccgtca gttaaaaaaa ggagaaaaat gcatagaggt tcatcatgag
    40861 agcccgaatg tgaggttcaa acgtggtgat aatgagatga tcttcaagaa tcatgtgtct
    40921 gatcacactg aactatatga aatcgtgaat caaatactgt ctgggatttt tgaattagga
    40981 ccttatgagt tcaaggaaga aaacggtaat ccaaaattaa atgaagaatg gcaagaaacg
    41041 attgaaacac aaagaaacaa tccacactta tttcaatagt cttccacagt atttcgtaaa
    41101 aatgttgatg aaaaagaaac accgtgaatc agaggtcgtg aaaaacctgc cctgcatcaa
    41161 gtaataagaa cagccaaaca aaaatagctc ctcctatttt tcgtttggtt gggcttatta
    41221 ccgcagatgc aagtttttga acaccgttta aggaagagac tgtgaagtca acgttctgac
    41281 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta
    41341 ataccgaggg tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag
    41401 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac
    41461 ttaataccga gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta
    41521 cttttgtcaa agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt
    41581 agtgacatct ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag
    41641 taatataaaa agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg
    41701 cattgagatg gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa
    41761 tatggaagat ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat
    41821 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg
    41881 tttgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
    41941 cgaagaaatg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca
    42001 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
    42061 aagttcttac gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc
    42121 gcaatggttt caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt
    42181 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
    42241 ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg ccaatgccga tcgttcaggc
    42301 ttatcaatca ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact
    42361 tagtccaaaa gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg
    42421 tgttcaatca gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt
    42481 atctaaccat ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata
    42541 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg
    42601 acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt
    42661 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
    42721 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
    42781 aataaaattg cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc
    42841 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
    42901 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
    42961 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
    43021 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
    43081 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
    43141 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
    43201 cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag
    43261 tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg
    43321 tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
    43381 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
    43441 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
    43501 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
    43561 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
    43621 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
    43681 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
    43741 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
    43801 cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg
    43861 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
    43921 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
    43981 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
    44041 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
    44101 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
    44161 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
    44221 ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc
    44281 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
    44341 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
    44401 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
    44461 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
    44521 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
    44581 tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
    44641 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
    44701 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
    44761 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
    44821 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
    44881 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
    44941 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
    45001 caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg
    45061 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
    45121 tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat
    45181 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
    45241 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
    45301 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
    45361 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
    45421 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
    45481 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
    45541 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
    45601 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
    45661 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
    45721 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
    45781 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
    45841 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
    45901 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
    45961 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
    46021 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
    46081 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
    46141 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
    46201 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
    46261 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
    46321 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
    46381 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
    46441 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
    46501 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
    46561 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
    46621 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
    46681 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
    46741 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
    46801 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
    46861 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
    46921 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
    46981 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
    47041 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
    47101 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
    47161 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
    47221 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
    47281 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
    47341 cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg
    47401 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
    47461 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
    47521 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
    47581 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
    47641 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
    47701 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
    47761 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
    47821 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
    47881 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
    47941 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
    48001 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
    48061 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
    48121 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
    48181 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
    48241 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
    48301 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
    48361 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
    48421 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
    48481 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
    48541 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
    48601 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
    48661 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
    48721 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
    48781 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
    48841 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
    48901 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
    48961 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
    49021 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
    49081 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
    49141 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
    49201 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
    49261 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
    49321 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
    49381 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
    49441 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
    49501 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
    49561 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
    49621 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
    49681 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
    49741 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
    49801 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
    49861 aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
    49921 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
    49981 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
    50041 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
    50101 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
    50161 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
    50221 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
    50281 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
    50341 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
    50401 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
    50461 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
    50521 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
    50581 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
    50641 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
    50701 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
    50761 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
    50821 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
    50881 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
    50941 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
    51001 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
    51061 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
    51121 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
    51181 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
    51241 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
    51301 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    51361 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
    51421 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    51481 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    51541 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    51601 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    51661 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    51721 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    51781 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    51841 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    51901 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
    51961 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    52021 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    52081 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    52141 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
    52201 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    52261 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
    52321 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    52381 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    52441 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    52501 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    52561 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    52621 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    52681 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
    52741 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
    52801 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    52861 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    52921 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    52981 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    53041 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    53101 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    53161 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    53221 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
    53281 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
    53341 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
    53401 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
    53461 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
    53521 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
    53581 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
    53641 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
    53701 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
    53761 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
    53821 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
    53881 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
    53941 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
    54001 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
    54061 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
    54121 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
    54181 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
    54241 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
    54301 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
    54361 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
    54421 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
    54481 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
    54541 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
    54601 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
    54661 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
    54721 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
    54781 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
    54841 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
    54901 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
    54961 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
    55021 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
    55081 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
    55141 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
    55201 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
    55261 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    55321 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    55381 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    55441 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    55501 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    55561 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    55621 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    55681 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    55741 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    55801 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    55861 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    55921 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    55981 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    56041 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    56101 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    56161 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    56221 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    56281 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    56341 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    56401 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    56461 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    56521 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    56581 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    56641 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    56701 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    56761 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    56821 ttccattagt gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga
    56881 gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta
    56941 aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct
    57001 gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa
    57061 taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt
    57121 agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac
    57181 gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg
    57241 gatccctata cctaaacttg aaaatatccg tccccatatg aaacggatcg agatcggagc
    57301 agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt
    57361 gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg
    57421 ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga
    57481 agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg
    57541 gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa
    57601 gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca
    57661 agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt
    57721 atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc
    57781 tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg
    57841 tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc
    57901 acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt
    57961 cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac
    58021 aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat
    58081 cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc
    58141 aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca
    58201 gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt
    58261 atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc
    58321 gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta
    58381 tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatacaatc gggcaaatac
    58441 gattaatgcc agcatggagt tcgatgtgga tacactaagt ccaacgtatc gtttgctgat
    58501 tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga
    58561 agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat
    58621 cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt
    58681 tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga
    58741 agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aacgaagtcg tgtcagaggc
    58801 ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca
    58861 aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca
    58921 agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa
    58981 accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa
    59041 cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct
    59101 gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga
    59161 atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct
    59221 aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat
    59281 tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca
    59341 tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat
    59401 tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa
    59461 atgctatact aatcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca
    59521 gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact
    59581 tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat
    59641 gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa
    59701 ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa
    59761 gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa
    59821 cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc
    59881 ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga
    59941 ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat
    60001 gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat
    60061 gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt
    60121 ccgctcatat tttactggca gccataatac gaaaacggaa cggcttgtta gtgaaatcga
    60181 agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct
    60241 gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt
    60301 ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaaag tactaaaaga
    60361 caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat
    60421 cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct
    60481 ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta
    60541 tacgaaaatc gttgatgaag tgaaaaagtt tttaaacgga ggacacgaaa aaatcgagtc
    60601 agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga
    60661 ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac
    60721 tgatttactg gatcgagatg tatttggtta tgcgattgac aaaggatgga tgtgtgtcca
    60781 agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta
    60841 taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca
    60901 tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt
    60961 gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc
    61021 gaataagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga
    61081 acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg
    61141 tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt
    61201 attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa
    61261 cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt cacgagtgat
    61321 tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga
    61381 tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc
    61441 aaaaaacgat aaacataaaa caaatgagtt gctattcggt atagatttgg ctgtagtacc
    61501 gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg
    61561 attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga
    61621 tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt
    61681 gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt
    61741 cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa
    61801 gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata
    61861 ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga
    61921 aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt
    61981 ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa
    62041 gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg
    62101 gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg
    62161 gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg
    62221 gaaatcaaga aaagagattg aaccaaaaat cgatttttgg atggaaaaat ttcaggtaaa
    62281 agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct
    62341 gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt
    62401 agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc
    62461 atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat
    62521 catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt
    62581 tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga
    62641 aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc
    62701 tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa
    62761 ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta
    62821 agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga
    62881 tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc
    62941 aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc
    63001 aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag
    63061 cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg
    63121 ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga
    63181 tacaacaaca actgactggc ttgcaatcga tgcttcgagc aagtcaatta ggcatttcac
    63241 cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg
    63301 attcaaaagg aaaaatgaca gttggagaag ataactcgac agtccaatat gtcgtaagtt
    63361 atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag
    63421 agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac
    63481 aaaagcattt ttacggaaaa ctaacaggtg ttttacttgt tgcattaacg caaatggcat
    63541 tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa
    63601 atatattaga cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc
    63661 tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg
    63721 tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg
    63781 gatatatgct tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca
    63841 cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag
    63901 tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga
    63961 tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt gtatagcgaa ggcggattat
    64021 ggagttcatt gaaacaatcg atttcaatta tgagaaatga acgaaaaaaa gcataaaata
    64081 tagaaaaata tagaaggaag ctggacattt attgttccca gcttcttttt tgtatagacc
    64141 tatttctatt tttttattat attggatata tatattcatt gaaaatagag cataacaact
    64201 ttataatgtt gagtatacca atttttggag gcgttttaat ggaccgctat ttagatttac
    64261 tttcagagaa gttccctaca acggaagatg tcatcacgga aatcatcaat ctcgaagcaa
    64321 ttttgcagct tccaaaagga acagagttat ttttgagcga tatacatggc gaatttcctg
    64381 cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt
    64441 tctctgaaca aatgacggaa acggagatca gccaattcac tatttttacg gcttatccag
    64501 aatatgtgtt gacgactgat tggtacaaaa aacaagataa atcccagctg atccatcgtt
    64561 tgatcgatct tttacggttt acgacagtca aatacactcg atcaaaagtt cgaaaaggat
    64621 tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgat cgtatcaacg
    64681 ggaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt cttgatgaag gaagaagaaa
    64741 aatttttaac aaaacttgcg cagacgatcc aaaaatctgt catcgatcat ttgcatatcg
    64801 tgggtgatat ttttgatcga ggaactcaag cagccaaagt aatggatcga atcatggatt
    64861 tttcctctgt agatatccag tgggggaatc atgatatttt gtggattggt gcgtattgcg
    64921 gctctgaggc atgtttattg acgcttttac ggattgccgc acgctacaac tatttatttg
    64981 aattagagaa agaatatgga ttaaatttac gtccattatt ttcttttgcc catcaaacct
    65041 atcagaagaa tccagtattt acccctaaaa acaggaaaga tctatctgaa agagagcaag
    65101 aagaattaga aaagatccat caagcacttt ctatcctcca attcaaatca gaacatcagt
    65161 tgttgaatcg acgacctgaa tttaaaatgg acgatcgtaa attgttggaa aaaatcaact
    65221 atgaggaatc aacgatcgtt ttagaaggtc agctctatga gttgaaaggg acttgttttc
    65281 aaacgatcca gccagatgat cctaaaaaat tcttggaaga ggaacaaaaa gtgatcgata
    65341 gtttgatgta ttcattccag cactcgttaa gaatgcaaaa acacatgacg tttttgatcg
    65401 aaaaaggggg aatgtatctc acgaacaatc agcatgtact gttccatggc tgtatacctg
    65461 tagatgaaaa ggggcagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac
    65521 tgctgggatt ttttgaaaaa cagataactg aaagcgcaaa agatttgact gcaaatgaag
    65581 atttggcgac cgatttgatt tggtatgcgt ggagcgggcc attttcgcct ttattcggca
    65641 agagtaaaat ggcaactttt gagcgctatt ttctcacaga gcctcatacg cacgtcgaaa
    65701 aaagcaatcc ctattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttggcag
    65761 aattcgatgc aaaagaagaa ggttcagcaa tcatcaatgg acacacgcca gtaaaagtaa
    65821 aaaaaggaga gtcaccgatc aaagcaaacg gaaaagcatt tgttattgac ggaggtctat
    65881 ccaaagctta tcaaaaaaca accgggattg ctggctactc attactatgc aattcttacg
    65941 gtttccaaat cgtgacacat tatccgttct tgcctattga acaacaattt gcaaaaaaat
    66001 ctgatcaaac gaatgtaaaa aaagtgatcg agcagcagct cccacgcaaa cttaatcgga
    66061 atacgacaaa aggaatggaa ttacagcaac agattactca actccaaagg ctgttggatt
    66121 atcgaaagga cgattgagaa aaatgctagc caacattgct caatagacta aaaagcgact
    66181 gcaacaaaag gcgtgtcgca gtctccttcg gaattagctc aaaaaacggt aaaatattag
    66241 aagctatttt tctgtttatt tctctaatac tcaggtcagg gcgctgattt cacgacctct
    66301 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc caacaaacat
    66361 gagaagctgt tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc
    66421 taatcagaaa aacgattaaa attcattcac aaagaaatcg aataaacgct gttcagatga
    66481 atctacacga tgagaaagtt ccggatgcca ttggacacca aggattttgt gcatattatc
    66541 tttactttgg actgcttcaa tgagtccatc gttggaaaag gcgatggctt ttaacaaagg
    66601 agaaagatct ttgactgcct gatgatgata ggaattcaca aagtatgaat cagggagaag
    66661 gtcacttaat aaactgtccg aaacgatttt gacttcgtga gttgcaaact gcggggcagt
    66721 gggctgttgt tcgtgtttta cggaccattt tggatataat gaaagatctt gataaagtgt
    66781 tccctccaac acgacattca atagttgaag tcctcggcag actgcaaaaa tcggttttct
    66841 ttgtttcaat gcttccaaga tcaatgcctg ttcgaattgg tctcgttgaa tatttgtttc
    66901 cgtcagtttt ggatgagggt cttccatgta aagttgcgga ctaacatcct gaccgcctgc
    66961 aagaatgagt ttgtcgattt tctcaattag ttgaggcgct gaatctggag acatgactgg
    67021 cagaataaga ggtaatcctt gagcatctaa aactgcatcc acaaatccct gcggtgtata
    67081 gcttacttga tttccttgga aagtatctgt cgctcgaatc agttgattcc cggcaatgcc
    67141 gatgattggt ttcattttta ttccccctaa agtagactaa ttgtaacaag gatttaaaaa
    67201 aatttaaact atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt
    67261 aaagagaatt ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata
    67321 ataaaaagta agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag
    67381 gggtgagaag gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca
    67441 tactttcaat gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa
    67501 gtgttagaag ctttggaaca aatccgaaaa gcagaagaag aaaatgagaa gcgtgctcga
    67561 accctacggc agcagttgaa gacctatgca gaagaaaaag agcaagaatt ggagataatc
    67621 caaacgaaca gtcggcaaca gctgaaacag ttggtagaag aaaaagaaca attcgaaaag
    67681 caagaattag aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact
    67741 ttgaaagacc aatatcaaaa gcatcagagt caagcaatcg gtgcaatcat tgaaagggtg
    67801 aaagaaactt atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa
    67861 tcaggagagg attttacaag cagtacaagg aatccaaaat gttgaaatcc gggatctgta
    67921 tcaaaataga acaaacaacc agtgggtaga aaaatatttt ccagatacca cgattattga
    67981 taaagaagca aaattaagcg ctttaggcaa tcggctgaaa gacatccaag aagctatcca
    68041 attcatacaa caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact
    68101 ttccttacaa gacttggaag caaattattc cgaagaaaat ttcatgaata aattagaaga
    68161 agtccttgat ttaaaagaaa agtgggaaca gctaactgaa aaacgtgatc gtttagaaga
    68221 agaggaagaa tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag
    68281 taagacgacc atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga
    68341 agaactaagt aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt
    68401 ttatctttcg tatgttgctt taaaaaaatc agttgctgct atagaagaaa ttgctgggaa
    68461 atacgggttc aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aattgcaaga
    68521 aaccaaacag caattatcca ttgttcacga gcagcaaaaa aagataacga cagcgttagg
    68581 tcagtgcagc ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc
    68641 agaacgggaa aaagtaaaag aacggtttat ccatgccact tatctgatcg ttttgcaagg
    68701 ttgggtagat gcagaagaaa aacagaattt gatgcatgtg ttaaacgaaa ctgtatcaga
    68761 agaagatata tacgttagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc
    68821 tactaaattg aaaaaccatc cgcttatcga accttttgaa ttgctgactg aaatgtacag
    68881 tctgccaaaa tatgaagaag tagatccaac gccttggatg acaccatttt atcttgtctt
    68941 cttcgggatg atggtcgcag atgttggtta cggattgctg atgctcattg ggacaatact
    69001 agctcaaaaa ctatttgttt taccccgcgg gatgaaacgt ttcgtaaaat tctttgagat
    69061 tctctcggta ccatcgatca tctggggatt gatctacagt tcatttttcg gacaggcatt
    69121 accaaaagaa ctatttggta tccgcttgcc tttcccaatc ctatctacga cggatgacgt
    69181 caatacgatt ttgattttat ccgtgatttt cggtctgata cagatcttgg ttggattatt
    69241 cgtggcagca aaggaaaata tcaggaggaa agattacttg agtgctgtca gtgatggttt
    69301 tgcctggcaa gggatcctga ttgggatcgt tattgcattg attggtgtca tgttgataaa
    69361 aaatacagca tttgtttatc ttggcgcggg gctatcgatc attgctgctt tatgtatctt
    69421 aatcgtgccg attatccaat cgccgtctaa agtcaagggt gctgccaaag gagcgtataa
    69481 tctttacgga ttgactggtt atatcggtga tttagtcagt tatacacgtt tgatggcact
    69541 aggtatttca ggcggaagta ttggcgcggc attcaatatg ctagttgctt tcatgccgcc
    69601 agccgctcga ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt
    69661 tttgacactg ttaagtgcct atgtccatgg tgcacgattg cagtatgtcg aattttttgg
    69721 aaaattttat acaggaggcg gacgtgcctt tgacccgctg aaaacggcgg aaaaatacgt
    69781 caacatcaat cataagaaaa aaaacaataa ataattggag gatttttata caatgatgga
    69841 ttacttaatt agtcaaaatg gtggaatggt atttgcagta ttagcgatgg caacagcgac
    69901 gattttttca gggattggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc
    69961 gttaacaaca agtcaacccg aaaaattcgg acaggctttg attctgcaat tgcttccagg
    70021 tacgcaaggg ttgtatggat tcgttatcgc ctttttgatt tttatcaact taggaaatga
    70081 tatgtcagtt gtacaaggat tgaactttct aggcgcttct ctgccgattg cttttacagg
    70141 tttgttttca ggtatcgccc aagggaaagt tgcagcagca ggtatccaaa ttttagcgaa
    70201 aaaacctgaa catgctacaa aaggaatcat ttttgctgcg atggttgaaa catatgcgat
    70261 tttaggtttc gttatttcct tcctattagt attgaatgcc taagcataaa ggaggaaaag
    70321 cagatgaacg ccattgaaaa aatcatctct cagatgaatg aagcagcaga gcaagaaaga
    70381 gcggcgttag agcaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg
    70441 caaatcgaaa cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact tgaaaaaaaa
    70501 taccgtcagc ttaggaatcg ccaacaagta gaagtacgac aagaaaattt gaatgcgaag
    70561 caggaatttt tacgacgatt gtttgcagat gcaatagctg aaatggagaa ttgggatgaa
    70621 tctgaacaaa tccagtttat caaaaattca ttgtattcat tacctttgac aggcaaagta
    70681 acattcattg ctggagaaaa atcagcagct tatctttctc agacattgct ggatgaatgg
    70741 aacaatgaac ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt
    70801 tttctgataa acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat
    70861 atccaaggaa cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt
    70921 gtatggaata ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca
    70981 atgagacatt tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga
    71041 aatccacgat ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc
    71101 ttttaaagga gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa
    71161 tcgtttggat ctatacgatg cgctatactt tccataatct gaaggttttg acaaaagccg
    71221 agatgacaaa tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa
    71281 ccttgaagga tgccattcat acgcaagttt caaatgaact ccctgaaagc attatggaca
    71341 gtatacgaga agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga
    71401 tttatgatcg acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag
    71461 aattgctgac agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag
    71521 gaatattgca gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta
    71581 tcgaaaaaga agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc
    71641 tattagagac agattatgct gattttctgc gccctgtcat ccgtgaggga aatcttgatc
    71701 tcgtaaagct tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa
    71761 cccaggcttt cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca
    71821 aaaaccttcg tttactggtt atcggcaaaa gaagccattt cgaaccagaa acaatcagaa
    71881 agcgggtgag acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt
    71941 ttgccattca aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt
    72001 caggcaattg aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt
    72061 gcagcattga gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta
    72121 ttgattccca atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta
    72181 gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt
    72241 ggaaaaatca taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt
    72301 atccaagaca tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt
    72361 ggcgatgtgg cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg
    72421 gttcgctcta ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg
    72481 tttgacggaa tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta
    72541 gggcgcggag ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca
    72601 gtagaagccg gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa
    72661 gttataaaac ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa
    72721 aaaggttcat tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa
    72781 aatttgacga tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta
    72841 aacccggatg cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca
    72901 aaaggaggag cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat
    72961 caaattgcaa aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga
    73021 aatgagatga cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa
    73081 tcgttgatgg aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga
    73141 gaagcttcta tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac
    73201 gttgctatca tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga
    73261 cgtttggaag aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg
    73321 gaatactatg aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt
    73381 ataaccgcaa tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa
    73441 aatacattgc gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga
    73501 cattttcctt cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag
    73561 tatatggatc atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt
    73621 ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca
    73681 gataatgacc gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa
    73741 aatgcttttg atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag
    73801 atgatcttga cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt
    73861 gagatcatgg acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg
    73921 gaaacagaat tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg
    73981 atcgtttcag aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt
    74041 cggtccattg atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt
    74101 ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc
    74161 aatggtccaa atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt
    74221 tttaggccat cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg
    74281 tttgggacga ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa
    74341 cggagaagta atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat
    74401 ctctgcaatc gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg
    74461 ctctggtctt cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga
    74521 ttcatcagat gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga
    74581 atttttcatg gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa
    74641 tttagcaaac gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc
    74701 agaatatctt gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa
    74761 ctatgcagag gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg
    74821 atatcctggg tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg
    74881 cggactaaaa ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac
    74941 tcatccaatc cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga
    75001 gctttacaag agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa
    75061 agataaggga actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct
    75121 ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc
    75181 tgctttatct gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata
    75241 tgtcaatcaa ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga
    75301 actgctatcg atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa
    75361 atatctgcca gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat
    75421 ggaattgact cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa
    75481 agataagcaa gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct
    75541 gcgtcaagaa atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc
    75601 ttcgatcacg gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt
    75661 gtctatcgta gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga
    75721 tgaacagctg acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga
    75781 tcggtcaatc gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga
    75841 aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt
    75901 agaatatatg acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga
    75961 agaaaacgag cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga
    76021 ctaagtggta tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag
    76081 aaacgtttgc cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc
    76141 ggcggcctac ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat
    76201 gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca
    76261 gagcattgga acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt
    76321 ggttttatga tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc
    76381 acacgtatta tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc
    76441 atgctttaca tcctaaaatt ggcctttgtt atccaattga ttggtacggt tgctttatct
    76501 tttgtatttg tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca
    76561 atctctagtt tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag
    76621 caaaatgttt atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc
    76681 atcgtatggc gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa
    76741 atcgcattaa ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca
    76801 gagcgcaatg gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt
    76861 tttatgagtg tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc
    76921 catgcaggtc tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg
    76981 gccggcggat tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg
    77041 aaaggacgta cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg
    77101 gcacttactt tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg
    77161 gtgactgaaa cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct
    77221 gcgtttggta cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa
    77281 ttgatcatta tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct
    77341 ctgatgacaa aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg
    77401 attggataat ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat
    77461 aatttttctt gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact
    77521 acccgttgtt gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga
    77581 ttcctttatt tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt
    77641 agtccagaag ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct
    77701 ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag
    77761 tcattctcaa ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg
    77821 acacgattcg ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt
    77881 gtaagactta atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg
    77941 atgacttcca catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc
    78001 ttgctttgga catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat
    78061 tttgtcattc cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg
    78121 atcgtatgtt gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga
    78181 ccttcttttt caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct
    78241 ataggattat tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc
    78301 tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa
    78361 tgatataact gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta
    78421 tcacctggag agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca
    78481 atagatagag attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta
    78541 gtcaattgaa aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg
    78601 ttattactag ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta
    78661 accagaccaa aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca
    78721 aagacattgg gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca
    78781 attccatatt tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc
    78841 tctctttggg agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct
    78901 aattgttgaa tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa
    78961 gttaacgggt aattgtcata agaattaata ggcattttct ccactcctta aaattgtata
    79021 ccatacaatt ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa
    79081 ttgtctatga aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg
    79141 attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct
    79201 cgattggtgg aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct
    79261 tatttggggg agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat
    79321 cagtatcatt taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta
    79381 cccaaagcat tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg
    79441 ctggcaacag attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt
    79501 caacaagcat tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt
    79561 gtttacgata ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat
    79621 cctaaaataa cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc
    79681 atttgtacgt tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac
    79741 gcacatatca atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg
    79801 atggaaactt ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac
    79861 ctgcttatag ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc
    79921 gaattagttt ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac
    79981 gcagttggtc tagctattga agatctttgt tgtgcagaat atatttataa caaattagag
    80041 aaaggggctt taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt
    80101 tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc
    80161 aaaaaattgc cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag
    80221 aatttgagct tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat
    80281 tagcaacagc atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt
    80341 ctactcaaag cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc
    80401 ctagtattat gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta
    80461 agattaaaga agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg
    80521 tggctaaatt gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca
    80581 tcgtgacagc agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa
    80641 caatcaatga agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag
    80701 aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag
    80761 aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag
    80821 gtacctcgtt tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa
    80881 ccttatagct acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt
    80941 tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat
    81001 actaactatc ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa
    81061 gaaggcgcag tgccaggtaa tccttttaag tcagtaatct caagaccggc agctttatgt
    81121 tgctcgtgta atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct
    81181 agatcatacg caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga
    81241 agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca
    81301 aaaccaaatg ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat
    81361 gtgtgagcca ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag
    81421 aagtaaggaa atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag
    81481 aaaataaaac tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag
    81541 caatttgctg attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag
    81601 attgcagtaa tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga
    81661 gaagagacga aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca
    81721 gaaatttctt cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact
    81781 tattattata atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca
    81841 actgaaaaga ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa
    81901 cgtggtagcc accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa
    81961 gcgagtagat cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc
    82021 ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag
    82081 agaaacggca tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt
    82141 ctcgttatct ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt
    82201 cgcaactgcc gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact
    82261 tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa
    82321 aatttctggt tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc
    82381 aggtgttcga tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct
    82441 gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac
    82501 acggcgtgcc atcacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat
    82561 gattttacct gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt
    82621 atcttgaaaa aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt
    82681 gcataaaacg atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt
    82741 tgttacttct cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat
    82801 agacataatt tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg
    82861 gctggcaaat tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc
    82921 aaccatttct tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc
    82981 aacaagctga ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat
    83041 ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga
    83101 attcacagca gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc
    83161 ttggactaat tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt
    83221 caaaaaacgt ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa
    83281 gtaagtgccc aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc
    83341 aagaaatggc agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa
    83401 ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg
    83461 gaagtacact tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat
    83521 gccactcatg gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt
    83581 aagatcaaat gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat
    83641 tcgatctgca tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt
    83701 ttggatcaca ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata
    83761 cacttctgtt tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa
    83821 gatatgttca aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca
    83881 taaagtcacc agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa
    83941 atttcgagaa aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt
    84001 aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg
    84061 tcgtagacgt tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg
    84121 gatatagtta tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata
    84181 aggggttgtc ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt
    84241 tgttccattg ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac
    84301 ttgcagaata cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa
    84361 ggggttggtc aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc
    84421 catttgtacc aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccgc
    84481 gtttttcctg attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc
    84541 gacgctgagc gataccggca aattttttgc catggatgct taaatcataa gttccaggac
    84601 aataagaatg ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca
    84661 ataggagcat ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga
    84721 aaatcaaaga aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc
    84781 tgatcactgg agcgtaacct gcatctttca gtgtttggat cccgtcactt agaaaaggag
    84841 ttcgaacatc cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga
    84901 tttgctggtg ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt
    84961 cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt
    85021 tgtataaatt catattttct acaatattat actctaaaat caaagagttg ttttggaaaa
    85081 aaggttttct gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt
    85141 agctatgaga agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat
    85201 caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa
    85261 agggcaaatg gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga
    85321 gacgggcgat gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat
    85381 ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc
    85441 ctatcgaaca ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag
    85501 tttgaaagac cgtcccacag gagggaaggt tcgaatcgat tatgatctgt tcatgggacc
    85561 ggaaaaaatc ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag
    85621 tttgattttt tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg
    85681 attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc
    85741 acacgcgatc ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat
    85801 ccaaaactat cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac
    85861 tgacttgaat attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc
    85921 atggtatgct atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga
    85981 agatactcca cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga
    86041 cgcgatcgat tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga
    86101 tgatctgttt gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt
    86161 gcaagaaatc ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga
    86221 agaagattta agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt
    86281 ttctgactct gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc
    86341 agcctatcag aataaggagg tgtgacacga t
//
LOCUS       DACDCV010000003        80744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDCV010000003 DACDCV010000000
VERSION     DACDCV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.003"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(228..311)
                     /locus_tag="IV041_000197"
     tRNA            complement(228..311)
                     /locus_tag="IV041_000197"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Leu,seq:gag)
     gene            694..1911
                     /locus_tag="IV041_000198"
     CDS             694..1911
                     /locus_tag="IV041_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8381020.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2095..2931
                     /locus_tag="IV041_000199"
     CDS             2095..2931
                     /locus_tag="IV041_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8381021.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3007..4155
                     /gene="nagA"
                     /locus_tag="IV041_000200"
     CDS             3007..4155
                     /gene="nagA"
                     /locus_tag="IV041_000200"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8381022.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4375..5433
                     /locus_tag="IV041_000201"
     CDS             4375..5433
                     /locus_tag="IV041_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8381023.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            5452..6471
                     /locus_tag="IV041_000202"
     CDS             5452..6471
                     /locus_tag="IV041_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8381024.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(6573..7289)
                     /locus_tag="IV041_000203"
     CDS             complement(6573..7289)
                     /locus_tag="IV041_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8381025.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7258..8043)
                     /locus_tag="IV041_000204"
     CDS             complement(7258..8043)
                     /locus_tag="IV041_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8381026.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8034..8783)
                     /locus_tag="IV041_000205"
     CDS             complement(8034..8783)
                     /locus_tag="IV041_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8381027.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9081..9605
                     /locus_tag="IV041_000206"
     CDS             9081..9605
                     /locus_tag="IV041_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381028.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            9621..11120
                     /locus_tag="IV041_000207"
     CDS             9621..11120
                     /locus_tag="IV041_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8381029.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11208..11843)
                     /locus_tag="IV041_000208"
     CDS             complement(11208..11843)
                     /locus_tag="IV041_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381030.1"
                     /translation="MIVKRPKRRISTQFGRFRLGMRTIKSALAVFLCILFFHLTQRGI
                     PMIAALSAVFSLRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILL
                     PILVIIVIVVSDGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAI
                     GINFFLRPPETEKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            11914..12870
                     /locus_tag="IV041_000209"
     CDS             11914..12870
                     /locus_tag="IV041_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8381031.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13175..14131)
                     /locus_tag="IV041_000210"
     CDS             complement(13175..14131)
                     /locus_tag="IV041_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8381032.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14305..14865
                     /locus_tag="IV041_000211"
     CDS             14305..14865
                     /locus_tag="IV041_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381033.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14858..15682
                     /locus_tag="IV041_000212"
     CDS             14858..15682
                     /locus_tag="IV041_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8381034.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            15692..16309
                     /locus_tag="IV041_000213"
     CDS             15692..16309
                     /locus_tag="IV041_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8381035.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16327..17010
                     /locus_tag="IV041_000214"
     CDS             16327..17010
                     /locus_tag="IV041_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8381036.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            17693..18928
                     /locus_tag="IV041_000215"
                     /pseudo
     CDS             17693..18928
                     /locus_tag="IV041_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            18936..19823
                     /locus_tag="IV041_000216"
     CDS             18936..19823
                     /locus_tag="IV041_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381037.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            19840..20676
                     /locus_tag="IV041_000217"
     CDS             19840..20676
                     /locus_tag="IV041_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8381038.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            20679..21302
                     /locus_tag="IV041_000218"
     CDS             20679..21302
                     /locus_tag="IV041_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381039.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21299..22591
                     /locus_tag="IV041_000219"
     CDS             21299..22591
                     /locus_tag="IV041_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8381040.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      22950..23047
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23163..23744
                     /locus_tag="IV041_000220"
     CDS             23163..23744
                     /locus_tag="IV041_000220"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8381041.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            23747..25054
                     /locus_tag="IV041_000221"
     CDS             23747..25054
                     /locus_tag="IV041_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8381042.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            25347..25835
                     /gene="purE"
                     /locus_tag="IV041_000222"
     CDS             25347..25835
                     /gene="purE"
                     /locus_tag="IV041_000222"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8381043.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            25819..26952
                     /gene="purK"
                     /locus_tag="IV041_000223"
     CDS             25819..26952
                     /gene="purK"
                     /locus_tag="IV041_000223"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8381044.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            26965..28260
                     /gene="purB"
                     /locus_tag="IV041_000224"
     CDS             26965..28260
                     /gene="purB"
                     /locus_tag="IV041_000224"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8381045.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(28440..28817)
                     /locus_tag="IV041_000225"
     CDS             complement(28440..28817)
                     /locus_tag="IV041_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381046.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29073..29801
                     /locus_tag="IV041_000226"
     CDS             29073..29801
                     /locus_tag="IV041_000226"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8381047.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            29801..30055
                     /gene="purS"
                     /locus_tag="IV041_000227"
     CDS             29801..30055
                     /gene="purS"
                     /locus_tag="IV041_000227"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8381048.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30057..30728
                     /gene="purQ"
                     /locus_tag="IV041_000228"
     CDS             30057..30728
                     /gene="purQ"
                     /locus_tag="IV041_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8381049.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            30729..32951
                     /gene="purL"
                     /locus_tag="IV041_000229"
     CDS             30729..32951
                     /gene="purL"
                     /locus_tag="IV041_000229"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8381050.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            32936..34375
                     /locus_tag="IV041_000230"
     CDS             32936..34375
                     /locus_tag="IV041_000230"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8381051.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            34407..35450
                     /locus_tag="IV041_000231"
     CDS             34407..35450
                     /locus_tag="IV041_000231"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8381052.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            35447..36025
                     /gene="purN"
                     /locus_tag="IV041_000232"
     CDS             35447..36025
                     /gene="purN"
                     /locus_tag="IV041_000232"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8381053.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36022..37557
                     /gene="purH"
                     /locus_tag="IV041_000233"
     CDS             36022..37557
                     /gene="purH"
                     /locus_tag="IV041_000233"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8381054.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            37573..38847
                     /gene="purD"
                     /locus_tag="IV041_000234"
     CDS             37573..38847
                     /gene="purD"
                     /locus_tag="IV041_000234"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8381055.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            38969..39883
                     /locus_tag="IV041_000235"
     CDS             38969..39883
                     /locus_tag="IV041_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8381056.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40016..41581)
                     /locus_tag="IV041_000236"
     CDS             complement(40016..41581)
                     /locus_tag="IV041_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8381057.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(41574..43025)
                     /locus_tag="IV041_000237"
     CDS             complement(41574..43025)
                     /locus_tag="IV041_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8381058.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43039..44055)
                     /locus_tag="IV041_000238"
     CDS             complement(43039..44055)
                     /locus_tag="IV041_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8381059.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44275..45363
                     /locus_tag="IV041_000239"
     CDS             44275..45363
                     /locus_tag="IV041_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8381060.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            45417..46943
                     /locus_tag="IV041_000240"
     CDS             45417..46943
                     /locus_tag="IV041_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8381061.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            46945..48150
                     /locus_tag="IV041_000241"
     CDS             46945..48150
                     /locus_tag="IV041_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381062.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            48407..49384
                     /gene="guaC"
                     /locus_tag="IV041_000242"
     CDS             48407..49384
                     /gene="guaC"
                     /locus_tag="IV041_000242"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8381063.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            49648..51072
                     /locus_tag="IV041_000243"
     CDS             49648..51072
                     /locus_tag="IV041_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8381064.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            51533..52642
                     /gene="pdhA"
                     /locus_tag="IV041_000244"
     CDS             51533..52642
                     /gene="pdhA"
                     /locus_tag="IV041_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8381065.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            52645..53622
                     /locus_tag="IV041_000245"
     CDS             52645..53622
                     /locus_tag="IV041_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8381066.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            53647..55290
                     /locus_tag="IV041_000246"
     CDS             53647..55290
                     /locus_tag="IV041_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8381067.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            55298..56704
                     /gene="lpdA"
                     /locus_tag="IV041_000247"
     CDS             55298..56704
                     /gene="lpdA"
                     /locus_tag="IV041_000247"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8381068.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            56866..57141
                     /locus_tag="IV041_000248"
     CDS             56866..57141
                     /locus_tag="IV041_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8381069.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57147..57914
                     /locus_tag="IV041_000249"
     CDS             57147..57914
                     /locus_tag="IV041_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8381070.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58082..59911
                     /gene="typA"
                     /locus_tag="IV041_000250"
     CDS             58082..59911
                     /gene="typA"
                     /locus_tag="IV041_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8381071.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60172..61944
                     /gene="recQ"
                     /locus_tag="IV041_000251"
     CDS             60172..61944
                     /gene="recQ"
                     /locus_tag="IV041_000251"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8381072.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62058..62360
                     /locus_tag="IV041_000252"
     CDS             62058..62360
                     /locus_tag="IV041_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8381073.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            62363..63526
                     /locus_tag="IV041_000253"
     CDS             62363..63526
                     /locus_tag="IV041_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8381074.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            63552..66980
                     /locus_tag="IV041_000254"
     CDS             63552..66980
                     /locus_tag="IV041_000254"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8381075.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67001..68125
                     /locus_tag="IV041_000255"
     CDS             67001..68125
                     /locus_tag="IV041_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381076.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68115..68552
                     /locus_tag="IV041_000256"
     CDS             68115..68552
                     /locus_tag="IV041_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8381077.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            68536..68874
                     /locus_tag="IV041_000257"
     CDS             68536..68874
                     /locus_tag="IV041_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8381078.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            68993..69553
                     /gene="rsmD"
                     /locus_tag="IV041_000258"
     CDS             68993..69553
                     /gene="rsmD"
                     /locus_tag="IV041_000258"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8381079.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            69550..70041
                     /gene="coaD"
                     /locus_tag="IV041_000259"
     CDS             69550..70041
                     /gene="coaD"
                     /locus_tag="IV041_000259"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8381080.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70031..71074
                     /locus_tag="IV041_000260"
     CDS             70031..71074
                     /locus_tag="IV041_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8381081.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71233..71961
                     /locus_tag="IV041_000261"
     CDS             71233..71961
                     /locus_tag="IV041_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8381082.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72077..72322
                     /locus_tag="IV041_000262"
     CDS             72077..72322
                     /locus_tag="IV041_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8381083.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            72319..72537
                     /locus_tag="IV041_000263"
     CDS             72319..72537
                     /locus_tag="IV041_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8381084.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            72673..73212
                     /locus_tag="IV041_000264"
     CDS             72673..73212
                     /locus_tag="IV041_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8381085.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73205..75478
                     /locus_tag="IV041_000265"
     CDS             73205..75478
                     /locus_tag="IV041_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8381086.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            75516..76562
                     /gene="holA"
                     /locus_tag="IV041_000266"
     CDS             75516..76562
                     /gene="holA"
                     /locus_tag="IV041_000266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8381087.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(76651..76905)
                     /locus_tag="IV041_000267"
     CDS             complement(76651..76905)
                     /locus_tag="IV041_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8381088.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(77305..77574)
                     /locus_tag="IV041_000268"
     CDS             complement(77305..77574)
                     /locus_tag="IV041_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381089.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78044..78499)
                     /locus_tag="IV041_000269"
     CDS             complement(78044..78499)
                     /locus_tag="IV041_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8381090.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(78637..80565)
                     /locus_tag="IV041_000270"
     CDS             complement(78637..80565)
                     /locus_tag="IV041_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8381091.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26190 a        13908 c        17504 g        23142 t
ORIGIN      
        1 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
       61 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      121 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      181 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      241 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      301 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      361 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
      421 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
      481 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
      541 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
      601 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
      661 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
      721 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
      781 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
      841 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
      901 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
      961 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1021 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1081 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1141 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1201 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1261 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1321 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1381 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     1441 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     1501 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     1561 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     1621 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     1681 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     1741 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     1801 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     1861 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     1921 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     1981 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2041 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2101 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2161 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2221 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2281 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2341 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     2401 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     2461 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     2521 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     2581 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     2641 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     2701 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     2761 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     2821 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     2881 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     2941 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3001 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3061 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3121 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3181 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3241 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3301 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3361 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     3421 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     3481 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     3541 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     3601 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     3661 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     3721 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     3781 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     3841 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     3901 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     3961 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4021 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4081 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4141 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4201 taaggttttt ttgtgttcta gtaaaaatca ctttacataa gtttcaaaag tttctttaat
     4261 ttcctattgt tcaataagta acaatgtaaa gataaaatta aaaatgttaa tacgtattag
     4321 caaaataaaa gcttttttta ttgtgaataa atgaacataa ttaggaggag aaagatgaac
     4381 ttatttgaca tttttcgatc gactttgaca gatatgtcga ttgtcagtgc aatcacttca
     4441 acagttttta ttatattatt agggtttgtt tgtagaaaaa aggggatttt ttctgaatct
     4501 gttggaaaaa ttctatcgaa agtcgtctta acagtggctt tgccagcact ggcatttcga
     4561 gctttcatgc aagatatcaa tacagaatta ctgatgcaag gaatcaatgt cttgatttgg
     4621 ggattagtta tttatattat tcttattcta gtttcaaaac cttttttctt aagctataaa
     4681 ggggataagc aaacaacgtt gcgtgtcctg acgatctttg gttctacgac ctttttcgga
     4741 acgcctatcg tcagtgcgat ttatggtcct gtcggcgtga tgtattcctc aattttcaat
     4801 attggctacc gaatcttttt gtattcttat ggatacataa aaatgagtgg cttgaagatg
     4861 gaagctaaaa atatcaaagc gatgttcgtc aatccaatca ttatcgctac ttttgccggc
     4921 cttttcattt ggctgttcca agggtatctg ccacaagtgt cagttacagg tgcagaagga
     4981 gagataagcc aagtagcgtt tttgcgaatt gaccagactg caccgtggct atttcagccg
     5041 atggattatt tagctggttt aagttcacca ttggcgtggc tagcaatcgg cgcaacatta
     5101 ggtgaagtaa gcttcaaaaa agctgcatct gaaaaaactt catggtatta cagtctagtc
     5161 aaagtggtat ttgtacctgc attgaatatc gtgattctag cagttttgac ggcaacgaat
     5221 attctaccag tcaattttga agcactggca acaatcgtta tcatgatggc tacaccagct
     5281 gcaacagtgg cagcagccta tgctatcagt tttgataggg aggcactttt agcctccaat
     5341 gcatccttac tgtcaactat ttttgctgtg gtactgacac ctgtatggat cgttgtatta
     5401 caaatgattc atggcatagg tttattttca taagaaatgg aggaagaaag tatgagaatc
     5461 gcatgttacg gtgttcgtcc gaacgagata gatttttttg aaaaattgaa tatttaccat
     5521 tatgatctca gtctttatga agaactgttg acacatgaca atatcgtgtc agcaaaggac
     5581 catgatgctg tattgttgag gggaaattgt atagcagatg agaccaatct tgcaaaaatg
     5641 caggaatatg ggattcgtta tgtttttaca cggactgtgg gagtcaatca tatcgatcta
     5701 caagcggcag ctgattttgg tatgacagta gcccgtgtac catcgtattc gccaaatgcg
     5761 atagcagagt tatctttgac ttttgccatg atgcttttga gaaatactgc gtatacgacg
     5821 atccgaacat ctttcaaaga ttttcgagtg gatgagcaaa tgtttagtcg tgaaatcaga
     5881 aattgcacag tagggatcat aggaacaggc aggatagggt tgacggaagc tgttttgttt
     5941 aagggactag gagccaaagt gatcggctac gatattcatc agacagaagc tgctaaaaaa
     6001 gtattgacgt tccactcgct tgaagagtta ttagccaaaa gcgatattgt cagtattcat
     6061 gtacctcata tcccaggtga aaacgatcaa atgatcaatg aaggattttt agctcaaatg
     6121 aaaaaaggta gcatcttgat taatacagcc cgtggagagc ttcaagataa tgaagctatt
     6181 ttaagagctt tgaaaagcaa tcatttagct ggttttgcaa ccgatgtttt tgcaaatgaa
     6241 acagaggttt ttttccgatc cttcaagcca tgggagacga ttccagaccc tgctatccag
     6301 caattagtag aaatgtaccc tagagttctt gtaaccccgc atgtaggttc aaatacagat
     6361 gaggcattat caaatatgat ttcgacaagt ttcgagaact ttcgtgaaat aatagaaact
     6421 ggaaaaacga aaaatgaggt atctcttcct aaagcccgtc aattaagata agaaaacatg
     6481 gatattcgtc aataaaatgt ttaaaaatag tccaagtaaa cagttgttac ttctgtatac
     6541 ttggactatt ttgtatggga taattcgatg actcaacagt aatctgtgag ctttttccgc
     6601 ccattctttg tcaattcata acaggttagc ggctggccga tttccaaata tttgattctt
     6661 tcgttcaata tatcttgttc tactaaaaaa ttcaaatatt tcttgacgga tatacgtgac
     6721 agtgagcttt tttgagccag ttcttttgtt gaaaaaatag tatcttcttt tagaataaat
     6781 gcacttattt tttttaaagt cagttttgac agacctttag gcaaatcgat gaatgatggt
     6841 atttcgtgaa tgggagtggt gtggttgaat acttgatcga tcaatttctg atcggctttt
     6901 tcagtcgttt ggattgtctg agagaaagca agatactttt cgatcgccag ctgaaaacgt
     6961 tgcaaagtga acggtttgat caaataatct aaggcaccgt agcggatcac attctttatg
     7021 gtttcgatat catctgcagc agtgatcaaa atcactggga tatcatttcc ttgctgtcta
     7081 agccaagata aaaaatggat accactttcg ccaggcatgt atacatcaag cagcagcaga
     7141 tctgcgtttt tttgtctgaa atattttttt ccttcttcta catttctgaa actttcgatt
     7201 acgttcatat tagggatgct ttccacaaaa gtacgattca attctgcaac cattggatca
     7261 tcttcgatta tcaggacatt tgtcatcatt ttgatcttcc ttttttggta attctagata
     7321 gacgattgta ccgccatttg tttgtttgga aaattctaaa aatccaccgt ggcgtttgac
     7381 gattgaatgg atgagattca atccaaaccc acgattgatt cccttcgtcg aaaaacctag
     7441 cgtaaatatt ttttctgaga tttcttctga tatcccgaca ccgccatcgc agacggtcac
     7501 gatgaatact tcagcttctg tgtcatagtt taaatgaatc cggacctgtc cgtttctttc
     7561 atgtttttgt agcacggctt cttttgcatt atcaagtaag ttccctaaca cttgcaaaag
     7621 tgaatggatg gagtcatcca tctccatttt agggataaaa gaatcgcttt ctaacagcat
     7681 ttcaacagat tgttctttgg cttcgtttat tttgcctaat aaaaaaccgg caattgcagg
     7741 tactttgatc agacttgaga cttggccaat ctctttttta taattttgat ttagctgctg
     7801 gataaaatgg gtgacttctt catacttctt caaatcaatc aaaccagaaa tgacatgaag
     7861 tttattcatg aattcatggg tttgagctct catggagttt atatattgtt tcgtcccccc
     7921 aagctcctcc attaatttca ttacttctga tcgatttcgt aaagttgcga ctgctcctaa
     7981 aaaacggtca ttgctaaaaa gaggtgtaac gctagaaata gtttctatcc catttaaata
     8041 catggattga gtccgaacgg tcgtttgatg ctcaatcgtc tctttaaaca aaacgtcata
     8101 tagattatca ctgatttttt tcctacttcg aaatccaata gctgctctgt ttggtagaaa
     8161 atgttttttg caggtgtatt gatcaagagg atagttttat cagaagagat ggctaagatt
     8221 ccttcatcta cttcattttc gataatcgtt ttttcactta actgttctgc tatttcttgt
     8281 ggttcaagtc caagcagtac ttttttcatt ttttgggcaa gaaaaacggc gcctatgatg
     8341 cctacgctaa gtccaataat caatgaaaaa acaatcattt tttgtccatc tttgacttga
     8401 ttgtcaatcg tgttgtttgt taagccaaca caaactgcac caataatctc ttgatcataa
     8461 taaataggga cgaaatagcg gaagccgaac cctaacattc cttctttttc agagaaaaat
     8521 tcttgtcctc caatcacttt tttcacatct tcatgacttg agaaaggaag accgatgtaa
     8581 cttgggtctg cgtgagaata tcgaatgcct tcagggtcca tgacaacgat aaaatctaaa
     8641 ttcgatgcaa tcatgatttc atttgttatt ccttgaatcg catgtgtcgg gtcaccagtc
     8701 tgcagctttt tttggatgag tttatcattg gcaacgattt tagcaatgct agagatttta
     8761 tctgacacac tagatcggtt catcgaaaga ataaaattcc tgatcaatat ggcagaaaca
     8821 atcaaagaaa cgaatgtgga gaaacaaacg acaaggatga tcgtgttctg gagctttaat
     8881 ggttttttct taaaaaaagg aaacataggc tacctcgaaa gaatttattg tgaaaatttt
     8941 aacatgaaaa caaaaattta acaaattgtc aacgataaac acaaccttcc tataaaaaac
     9001 accactgagt cggtaaaaga agctatacag aattttcaaa cattggtatg atagacgtag
     9061 aaatggaagg aggcgtgtat atgatagggg gaatcgcact tgagacgatt tattggtata
     9121 cattgctgat ttgtgcagga ttggcagtcc tattgattat ttttggagat gtattcgatt
     9181 ttgatggccc gcttgatccg atgcttatta tcccttggtt aactttcaca agcttatttg
     9241 gttttctagg tgagcgttta acagatcaat catctttatt tttatttatt gctagtggag
     9301 gcatatcgat agtgttggta tttttgctta atttctatat attgatgccg atgaaacatg
     9361 ccgaatcaac gatgtcgtct tctgaaaaaa ctctggaagg acaagtagct gttgtgatca
     9421 caccgatccc tgtacgaggt atgggagaaa ttcagttgaa aagtgttacc ggttccatta
     9481 gtcgtccagc ttgtttttac agcccccaag aacagacagc tgagcgggga acgaaagtat
     9541 tgatcattga gatcaaggaa agaatctgtt atgtcatgcc ttatgaaagc atgataaaag
     9601 cttaaaggag cgtgtgttaa atggaaatac cagttgtttt tatcgcgatc gcaatcgcag
     9661 ctttgatttt actgatgttg ctgatcgtct ttgtagcgaa gtaccagaca gcaaaaccgg
     9721 atgaagcgtt gatcatcagt ggaagttatt taggaagcaa aaatgtgcac attgatgaaa
     9781 gcaataacaa aatcaaaatt gtgcgtggag gtggcgcatt cgtcttgcca gtttttcaac
     9841 gttcgaatcg gatcagtcta ttatcgagta aattggacgt ctccacccct gaagtgtata
     9901 ccgaacaagg cgtaccggtg atgtgtgatg gtacttcgat catcaaaatc ggttcatctg
     9961 tagaagagat tgctacagca gcagaacagt ttttaggcaa gacaagagaa gaactggaaa
    10021 atgaagcaag agaagtatta gaaggacact tgcgctcgat tcttggttct atgactgtag
    10081 aagaaatcta tcaaaatcgc gataagttca gtcaaagcgt acaagaagtc gcttctgtag
    10141 accttgcaaa aatggggttg atcatcgttt ctttcactat caaagaggtc agagacaaaa
    10201 acggttatct tgactcttta gggaaaccaa gaatcgccca agtcaaaaga gatgcagata
    10261 tagcagaagc ggaagctcta aaagaaacgc gtatcaaaaa agctcaagca gaaaaagaat
    10321 cacaagcagc tgaattacag cgacaaacag agatcgcaga atcattaaaa gaaaaagaac
    10381 tgaaattagc aacctacaaa caagaacaag atgttgctaa agcaaaagca gaccaagcct
    10441 acaatttaga aagtgcccga gcacaacaac aagtaatcga acaggaaatg cagattaaaa
    10501 tcgtagaacg ccaaaaacaa atcgaactgg aagaaaaaga aatcacacgt cgtgaaaagc
    10561 aatatgattc ggaagtgaag aaaaaagcag atgccgatcg gtacgctaaa gaacaagaag
    10621 cacaagctca aaaagtcaaa gaagtgactg aagccgaagc agaacgattc cgtgtggaag
    10681 cactggcaga agcggaagcc aataagacac gtttagctgg acaagcagaa gccgaagcag
    10741 cactagcaaa aggaaaagca gaagccgaag caaaacaaaa gatcgccaat gccttcaagg
    10801 aatatggcga agcagctgtc ttgagtatgg ttatcgatat gctaccgcaa ttgatgcgcg
    10861 aagcagcaca gccgctagga aatatcgaaa aaatctctgt tgtcgataca ggaagcagtt
    10921 caggagaaac cagtggagcg aaccgagtga caaattatgc cacaaatttg ctttcttcta
    10981 ctcaagaaac actaaaagcg acaacaggac ttgacttgaa ggaaatcgta gagaatttgt
    11041 cgacacgtgg gactactagc caagtaaata tgtatgaagc gccaaaagag gcagaacaaa
    11101 actcgatgaa ggaagaataa agtagtacac ttcaccgttc aatcaaaaat ggaggaagac
    11161 tttttaaagt tcttctctcc atttttttat tttattgcaa aagaaaatta gtcctcgcta
    11221 tttttctttt gtttttggat cttagcttct atctcttgta aatttgttct tagcaatcgt
    11281 tctttctttt ggagttcgac caaatcttct gatagttctt ccttcttttc tgtttcagga
    11341 ggacgaagaa agaaattgat accgattgca atgaatgtcc ctataaaagt atctagtaca
    11401 cgttggatag catacaaagc ggattcgcct tgagggatgc tcaacgcaat cagcaacaac
    11461 gtagctattg cggaaatgat accggaatta ttgttaatac catctgacac gacgatcaca
    11521 ataataacta gaataggaag caagatcaat tctaccaaaa aatcattgtg aaaatagctt
    11581 ttgacaaaaa aataaatgat agctaatgta ccacctaatg tattgccgat gatcctagac
    11641 tttccaaaag aaacggttgt agtcaagtcc tgacgcaaag aaaaaacagc agataatgca
    11701 gcaatcatcg ggattcctcg ttgagtcaaa tgaaaaaaca agatacataa aaaaacagct
    11761 aaggcacttt tgatcgtacg catacctagt cgaaaacggc caaattgcgt agagattctc
    11821 ctttttggtc tttttactat catacctaaa cttttgtctg atagctagtt tttcattatt
    11881 caagtataat taaaataaaa aagggggaag taaatgcgta taggaatgcg aacagtcaaa
    11941 acagtgatca gtgtcttctt ttcgatggtc acagcgggta gcttatcgct gttgtattgg
    12001 ccagctgcag ggatcattgc attattaagt gtaggaaata ccaagcgctc tacattgcgt
    12061 acgggcatga atcgaatcgc agcctttata ttagctacgg caacagcact gttcagtttt
    12121 tttgctgttg ggtatactat ttttggcttc agtctttttt tattcctgtt catcccgctt
    12181 gcctctcgct tcaaattaac cgaagggata gtagtaaatt cggttttggt cactcattat
    12241 ttagtggagg aaaacatgtc ttggcagctg atcggcaatg aagcgggatt aatggcgatt
    12301 ggattagtat ttgctttgct tgcaaatgtt tacatgccag atcgtacgaa gcagctgaag
    12361 gaaaaccaaa tacagatcga aaaagaattt cgaaatattt tgagacaaat ggcagaattt
    12421 cttttatcag aaaataaaga agatgtgcag atcaaatgcg aacatttact gacattcatt
    12481 cgcgaaagcc aagaagatgc cagagaacat caagaaaact actggctcag acagccgcta
    12541 tactacgaaa cttacttctc tatgagaaga gcacaggcca atgtgattaa agacatgctg
    12601 gaaaatctag agagaatcca gcaaccagct tattatggaa aacatatcta tggtttattg
    12661 atttatactg cagaaacatt ttcagagtca aatgatggca gacaaatttt aacacgtatt
    12721 gaagaagttt atgtattgta tcggcaaatg cctctgccga ctagtcgccc tgaattcgag
    12781 gatcgagcag aattgttcca gtttttgcag tcttttaaaa gttttattga aatcaaggca
    12841 gaattctccc aacaaaaaat ccaaaaatag aggttgtgaa ttcagctaat attcaggtca
    12901 aaaagctgat ttcacaacct cttctttaaa cggtgttctc ctgtcaactc ctcggtcaca
    12961 agtcacaata ttcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcgga
    13021 gctgaacggt aggctcacaa cctctttctt aaacggtgtt caaaagcttt cctctgcggt
    13081 aataagtcca gccaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
    13141 tgatgcagaa cagctttttc acaacctttt aaaattacat atttttccat agaacttgca
    13201 tttgatccaa gatttcgatg acatcttttg tcaagaagag agagcgattg ggcaagtcac
    13261 cttcaatcat gtgtaccata ttctttattt cgtaattcat tgcttcagat gtattcccgc
    13321 tttcgatgaa ttcctttgta ccatctgtaa ataagatctc cgcacgatca gcacgcgggt
    13381 aatcagcaat cgtcacataa ccgttctcaa aggcaacgat accgactttt ggcatttttg
    13441 cctgaaaagt caagctgaca gcagcaagtt cattttcttt gttacgcaag atagtgacgg
    13501 attgttcgtc cacgcctgtt tcaaaaggaa ccattgtaga ggcaactact tcaggttgag
    13561 agctcaaaaa gaaacgagcg aaagatacag catacgtacc gatatccaac agagctcctc
    13621 cagctaattc tggattgaag aaccggtttt tagggtctgg ttctttgtaa ctgccgaaag
    13681 gtgcttggat catttttaaa gcccccagtt ttcctgtatc cattattgag cgtaactgct
    13741 gataaagtgg catgttgaag attgtcatcg cttctgctaa gatcaaattc ttttcttctg
    13801 ctaagcgcat agcgtcagcc agttcttttt tattcatcgt gatggctttt tcacatagaa
    13861 cgtgtttgcc tgcttccaat gctgccaaga tatggtcgat atgctgtcta tttggtacag
    13921 caatgtacac gatgtcgatt tcagcatcgt ctaacatttc ttgatagctt ccgtaagctt
    13981 ttggaatatt gtaacggtga gcgaagtttt ccgctttttc tgaagttctg gaagcaactg
    14041 ctgttagttc gctcgtctct tgattgaagc tttcggcaaa ctcatgtgcg atggtaccaa
    14101 gaccaacgat tccccattga tattttttca tctgaaaacg ctccttcata gttggattga
    14161 gtttcttgtc tgtctattat tataacaaaa atcacgaaga ataaagagga gaggacttga
    14221 aacattaaaa aaaatgaaag cagcgattta agaggtgctt tttgatcaaa acttaagtag
    14281 aatagttaaa aggaggggat aaacgtgcat caagtatata ttgatcttat tatgagtgta
    14341 ttattagaac agtttgaaac tgaaaaaaat tttttagatg aatacttgct gatcagtcca
    14401 gaacaatggc aaaaatggaa agatggcgag aaaaatctgc catctgaagc gatgcagaaa
    14461 gtaaaaaatc ttttcactga ttatgaatgg atgctaacgc aaaagatttt gcgtcaaacg
    14521 attatttttc ccgaaaaacg aaacgttgcc gttgcagagt acaagcaaat caaaacacgc
    14581 atcgcgcaaa aatggcttag cagcaaagca gcaaaagtcg agctgatccc tttgaaaaat
    14641 aatcagcagg ctggtagata ccttgacttg aaagtaagta ttcaatatga tgaatggggc
    14701 tatgatgata ttttgaattt tcgtctgcca gcagatgttc aaaaacagat cgaaggagaa
    14761 aaaatcgaac ttctcgattg ggtgaatgaa aatctggaag aaacttacgt tcatgaggcg
    14821 aatggggaac gtgaagaaca acgaaaggat ttttcaagtg aataaatttt taggatttgg
    14881 agcagttgtg attatgatcg cagcaatggg ctatgttatt tttagcgatg atgattcgca
    14941 tgttcaagcc ggcaatcaag cagtcgaacg aacaagcacc aaaacaactg ctagtaaaga
    15001 aaagaacagt caaacaacca gcagcaaggt aaaggacaaa gaaacaaaag atcgtacgtc
    15061 caaacaattg ccagatgtta aaacagacga ttggaagcta gttttggttg gtccagatca
    15121 caaattagaa aaagaaattg atgagtctac tcaattggca tcattaagta acggatactt
    15181 gatcgacaaa cgcattgctc cagaatatga agcatttgcc aaggctgctg aagcagctgg
    15241 atttccatta gtgatggtct cagcttaccg gtctgtatca tcccaacaac aagttttcga
    15301 acaaaatgtc caagatgtga tgagtcgtca aggtgtatca gaagaggagg cgacaaagat
    15361 tactaaagaa acgattacgg agccaggata cagcgaacac cacacaggtc tggcagtcga
    15421 tgtcgtcgat caaaactggt acaacagtta tccttctact gtattggatg caagttacgg
    15481 agatcagcct ggagctaaat ggatcgcaga taatgctgcg cagtatggat ttatcgttcg
    15541 ttatccaaaa ggacacgaag atataactaa gatcacatat gaaccttggc acttacgtta
    15601 tgtcggtaaa gaaaacgctg agtatatcac caaacaccaa ttgacgctag aagaatatct
    15661 gaatgaatta aatgaaaagt aggaattgat catggcgaaa aaaaagtgga aaaagcaaaa
    15721 gcgcaagaat atccgttggc cagcagtggt tgccggtgta gcagtgatac tcttagcctt
    15781 tgttttttcc ttgaagaatc tgtcttctcc ttttactgat aatcagcaag aaaatgaagc
    15841 gcaatcccat cagcaattta ttgaacggct tgttccgcat gcccaagaac tacaagatgg
    15901 ttatggagtg cttcctagta tcattttagg acaagctata ttagaatcaa attggggaaa
    15961 aagtaccttg gctagcaaat acaataatct tttcgggatc aaggcttatg gggatcagaa
    16021 gaaagtaagc ctggaaacaa aggaatttgt taacgaagaa tggataacta tccaaggaga
    16081 cttcaaagtg tatgattcat gggaacagtc catggatgac catacacaac tgttcgtcca
    16141 aggagtagat tggaatcctg cgctgtatga aaaagtgatt acggccacca actatcagga
    16201 agccgcacaa gcattgcaag atgcaggtta tgcgacagac cccggttatg cgcaaaaaat
    16261 catccaggtc atcgagacgt atcaattgaa tcagtatgac cggagatagc aaaggagaag
    16321 tggctgatgg cagaacaatt tacgccagaa atcatgacgg aattgagaaa agatatcgta
    16381 agagtaccgg aaatcatttt agaagcaagc gggatcaaga tatttggaaa attgatccgt
    16441 tcgatcatct ttactacgga tattgcgatc atacgtaata caaatgcgaa tgcagtaatt
    16501 gctgtgtatc cttttacacc tcatccagcg attactaaaa gcattattga ggcagctgac
    16561 attccagttt tctccggtgt aggtggtggt ttgacacaag gtgctcgttc ttcctacatg
    16621 agcatgttcg cagaagcgca aggttcgtta ggggtcgttt tgaacggtcc tacgccttta
    16681 gagacagtaa aagaagtttg tcgagtagtt gatattccag ttatcagtac ggtaacgtcg
    16741 aaatatacag aaatagatga aaagttagca gctggtattt cgattatcaa tatcagtgca
    16801 ggtaaagaaa ctgcagaagc agtacgccat tttcgtaaaa aatatcctga acttcctatc
    16861 attgcgacag gtggtccaac ggaagaaagt attgctgaga ccattcaagc aggagcaaat
    16921 gccatcacat atacgccacc aagtagcgga gagcttttta gtcgtaaaat ggaaaaatat
    16981 cgggaaactg agaaaaagaa tcatgaataa caggtgagaa tcaaaagacg aggaagagca
    17041 gatagtggct cttcctcgtc ttttgtactc cagtactatt tgttcagaat tcaaaaaaaa
    17101 gagaaatttt tttgtttttt tacctgtaat gtacataaga agagttaaaa ccttttgtta
    17161 ttattagcat tctaacaaaa aaatgtcttg atttattgaa gcgctttcta tagaatgaaa
    17221 aacgaataaa aactaaaaaa ggcacccgac aatttagtag cagctaaatt gtcggggctt
    17281 gaaaagtcag cgggaactga ccaatcaagt tgccatagtc aatgcttgtg catcgacgtg
    17341 tatattgtac ctaatttcat acagaattac tagttatttt tctgtttttt agggaagagc
    17401 gcacacgaag aagcatgctt atttagctat gtcaaggata ttgggcagtt gtctgatatc
    17461 cttttttctt tgcgtaatta tcctcccatc attgaagcaa gaagtgtttt tagcgatgaa
    17521 agaagaatca gcactatcta atttttcttg aaagtattga gttgttcatc ctaggcagaa
    17581 cgataaggca agtcttggtt tttattcccc ttgaaaaaga tcgtttaata aatatgttct
    17641 gtataaaaat acagtatatt gaaaaataac aatggaagta gaggtgcaga gaatgaagaa
    17701 aagaatgaga atcatcttag ctttaagtgt tatgttattc ttcttgacgg cttgtgggca
    17761 agaagatgac aaaaaaatca cgctgaacat ggcttggtgg ggttcgcaag tccgacacga
    17821 tgcaacagta aaagtaatcg agctttatga agaaaaaaat ccccatgtga cgatcgatta
    17881 tgaattttat gattatgaag gatattacac aaaattaggt acactagcag catcagatga
    17941 tatctgggac attttccaac tgaacaatca atttcctcaa tacatcaatc agattgaacc
    18001 attagatgaa tatgtggagc aagggataat tgatatttct aatgcagatg aatcctattt
    18061 agcaacgacg acttacgacg ggcaattagt tggactgtct aacggggtga attcgtttgc
    18121 gattgcttac aataaaaata tatttgaaca attaaatatc cctgaaccag cttcaaattg
    18181 gacatgggat gaatttgaac aaatcagcta tcaaatcact gaagaattag gaatctatgc
    18241 tatttcaaga ttcgaagatt ttatagctgg ctgtattatt caaataccac aagttgagaa
    18301 aggattgaat ttttataatc gggaagatct atctaacagc ttgggatttg aaaatccaga
    18361 ctatctaaca gattttttcc agctgcgtaa aaatctggta gacaaagggg cccatccaga
    18421 accaggtggt ggagctgcaa gtacgacgga tctgtctttt caggcggtta gggatagaga
    18481 agcggcaatg gtgtttctat caagtaacca gttgacagaa atattgtcag gggctcctga
    18541 agatatggag attcagttga ttaatccacc tcgcagaaaa gcagatggag aatctggtat
    18601 ccctttacgt tcttcgcaaa tgctgagtat ggcaaaaagc tcagcccata aagaagaagc
    18661 tgctaagttt attgatttct ttcaaaacag tgaagaagcc aatgaaattt taaaaggaga
    18721 acgtggtgta ccaatcatgt cgaatattcg agaacagctc gctcgtgcag atgatgcaac
    18781 tttgcaagcg acatttgatt atttagaaat gatttccgat atggacaacg gcgaagtgaa
    18841 tgtctttgat agtgctttga atccggaaat tcaagaccaa tacaatcttt tagttgaaaa
    18901 agtcatttat gaagaagtaa cagcagaagg aaggcatgaa aaaagaaaaa ctaaaacggt
    18961 tcttggatca agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc
    19021 tattgataac agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt
    19081 tgggaaatcc agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc
    19141 gttttttgaa ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac
    19201 tgatctttgg tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct
    19261 ttcgttcttt gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt
    19321 ggcgggagtt gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac
    19381 ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg
    19441 cttggcaatt cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct
    19501 cgtattatga agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca
    19561 cgttacctct tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt
    19621 ttatgacttt tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact
    19681 tttatgctct ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg
    19741 ctatttcgtg gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat
    19801 caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa
    19861 tggtttcaat acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg
    19921 atgatcgcag gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt
    19981 ccagagactt ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca
    20041 ttttttactt tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt
    20101 ctgagctcga cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc
    20161 tggttcgtcg tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag
    20221 tttattattt acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat
    20281 ttttttggac aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa
    20341 gagttagatg aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt
    20401 atttttcctt tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa
    20461 tgggacgact tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg
    20521 attgccatca aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg
    20581 atgtctacgt tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt
    20641 gtccagggga tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga
    20701 tgtttcttta caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta
    20761 atgctctttt cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc
    20821 gtgttgtcta tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt
    20881 acttggcaga aaaaaaggta tgttggagga aagggaaaat caaacggatg tgtttcatca
    20941 ttacgttgtt tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa
    21001 agattggagc ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata
    21061 gtttttttag tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt
    21121 tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat
    21181 gccttttgta tggactgttc tggtgtatac agcctgattt tttactcact gcattactgt
    21241 atccagggct gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat
    21301 gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat
    21361 tgcctctacg tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag
    21421 gatggattat gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg
    21481 tatgccatca caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac
    21541 gtcagtagat cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt
    21601 tatttgcgaa aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca
    21661 aaaacagacg ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac
    21721 gaagataaag gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga
    21781 atggctgtta aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca
    21841 caatagcggc gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg
    21901 gttgaacagc cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca
    21961 taatgcggag aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc
    22021 taaagctgat ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt
    22081 agaagcagaa aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc
    22141 tattgaagat acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc
    22201 gctaaacgcg tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg
    22261 gatcgaaatg aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga
    22321 aggagcagcc gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt
    22381 aatcgtcgaa caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct
    22441 atttggtgaa gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat
    22501 tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt
    22561 gattgacgag cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta
    22621 gtttataaaa tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa
    22681 aaggggctat gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata
    22741 ggagaaaata aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat
    22801 tagattttac aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg
    22861 ttttcaatat ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa
    22921 acagttgcaa gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt
    22981 cataatatgg atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt
    23041 ggctatttca cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca
    23101 tagtaggttt ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata
    23161 aagtgaaaga attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat
    23221 taaaagtcga cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa
    23281 aacggtttgc agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt
    23341 ctgggatcgc accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta
    23401 gaaaagcaaa aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta
    23461 cgaaacaagt gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag
    23521 tattgattat cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat
    23581 gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag
    23641 atggacgtca gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt
    23701 ccttgacgaa tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac
    23761 acaaaatgga aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc
    23821 agtagcggtg cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta
    23881 tttgatttca attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa
    23941 ccgatttttc ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc
    24001 actgatcttg atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc
    24061 tgggatcttt gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc
    24121 tatagtaaca ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga
    24181 gaaaatgggt ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact
    24241 tgcatttgtg acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg
    24301 ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat
    24361 gattgatctg agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt
    24421 tggtaaacca actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag
    24481 catggtagaa tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg
    24541 agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat
    24601 tttcaataca ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg
    24661 aatcaaaaca agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt
    24721 ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt
    24781 agtcatgttc gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag
    24841 caatgataaa aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt
    24901 tacgccgaca ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat
    24961 cgtaatgagt agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga
    25021 tgaagaaata ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc
    25081 agcgttttga cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt
    25141 ttgtttgagc taataccgag ggtcagctga ttgaacaccg tttatccata aaaaggaatg
    25201 tgacctaact tttgtcacat tccttttttt catctttaat aaacacgaac aataaaatgt
    25261 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    25321 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    25381 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    25441 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    25501 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    25561 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    25621 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    25681 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    25741 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    25801 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    25861 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    25921 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    25981 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26041 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26101 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26161 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    26221 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    26281 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    26341 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    26401 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    26461 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    26521 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    26581 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    26641 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    26701 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    26761 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    26821 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    26881 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    26941 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27001 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27061 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27121 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27181 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    27241 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    27301 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    27361 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    27421 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    27481 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    27541 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ctctactcaa
    27601 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    27661 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    27721 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    27781 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    27841 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    27901 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    27961 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28021 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28081 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28141 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28201 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    28261 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    28321 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    28381 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    28441 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    28501 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    28561 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    28621 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    28681 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    28741 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    28801 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    28861 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    28921 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    28981 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29041 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29101 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29161 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    29221 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    29281 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    29341 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    29401 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    29461 catttatcaa tgaagagcat atctatttct tacaacttgc aaaaccggaa gaaatcagtg
    29521 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    29581 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    29641 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    29701 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    29761 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    29821 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    29881 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    29941 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30001 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30061 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30121 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30181 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    30241 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    30301 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    30361 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    30421 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    30481 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    30541 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    30601 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    30661 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    30721 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    30781 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    30841 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    30901 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    30961 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31021 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31081 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31141 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31201 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    31261 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    31321 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    31381 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    31441 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    31501 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    31561 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    31621 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    31681 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    31741 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    31801 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    31861 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    31921 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    31981 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32041 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32101 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32161 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    32221 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    32281 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    32341 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    32401 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    32461 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    32521 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    32581 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    32641 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    32701 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    32761 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    32821 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    32881 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    32941 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33001 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33061 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33121 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33181 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    33241 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    33301 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    33361 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    33421 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    33481 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    33541 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    33601 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    33661 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    33721 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    33781 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    33841 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    33901 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    33961 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34021 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34081 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34141 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34201 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    34261 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    34321 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    34381 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    34441 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    34501 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    34561 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    34621 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    34681 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    34741 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    34801 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    34861 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    34921 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    34981 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35041 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35101 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35161 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    35221 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    35281 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    35341 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    35401 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    35461 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    35521 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    35581 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    35641 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    35701 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    35761 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    35821 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    35881 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    35941 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36001 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36061 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36121 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36181 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    36241 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    36301 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    36361 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    36421 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    36481 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    36541 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    36601 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    36661 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    36721 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    36781 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    36841 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    36901 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    36961 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37021 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37081 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37141 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37201 ccgactccag cagaagcaaa agcattagcg tttgcttgga gagctgtcaa acatgtgaaa
    37261 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    37321 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    37381 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    37441 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    37501 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    37561 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    37621 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    37681 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    37741 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    37801 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    37861 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    37921 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    37981 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38041 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38101 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38161 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    38221 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    38281 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    38341 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    38401 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    38461 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    38521 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    38581 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    38641 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    38701 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    38761 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    38821 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    38881 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    38941 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39001 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39061 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39121 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39181 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    39241 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    39301 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    39361 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    39421 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    39481 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    39541 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    39601 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    39661 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    39721 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    39781 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    39841 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    39901 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    39961 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40021 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40081 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40141 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40201 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    40261 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    40321 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    40381 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    40441 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    40501 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    40561 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    40621 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    40681 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    40741 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    40801 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    40861 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    40921 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    40981 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41041 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41101 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41161 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    41221 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    41281 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    41341 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    41401 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    41461 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    41521 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    41581 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    41641 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    41701 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    41761 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    41821 ttgaattgga tcatctcatc aattttgcat gcggtaatac gaataaatcc tttgtgacgg
    41881 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    41941 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42001 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42061 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42121 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42181 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    42241 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    42301 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    42361 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    42421 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    42481 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    42541 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    42601 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    42661 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    42721 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    42781 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    42841 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    42901 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    42961 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43021 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43081 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43141 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43201 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    43261 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    43321 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    43381 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    43441 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    43501 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    43561 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    43621 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    43681 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    43741 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    43801 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    43861 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    43921 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    43981 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44041 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44101 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44161 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    44221 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    44281 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    44341 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    44401 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    44461 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    44521 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    44581 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    44641 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    44701 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    44761 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    44821 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    44881 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    44941 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45001 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45061 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45121 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45181 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    45241 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    45301 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    45361 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    45421 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    45481 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    45541 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    45601 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    45661 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    45721 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    45781 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    45841 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    45901 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    45961 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46021 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46081 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcggg atggtgggca
    46141 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46201 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    46261 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    46321 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    46381 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    46441 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    46501 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    46561 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    46621 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    46681 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    46741 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    46801 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    46861 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    46921 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    46981 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47041 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47101 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47161 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    47221 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    47281 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    47341 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    47401 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    47461 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    47521 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    47581 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    47641 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    47701 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    47761 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    47821 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    47881 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    47941 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48001 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48061 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48121 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48181 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    48241 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    48301 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    48361 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    48421 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    48481 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    48541 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    48601 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    48661 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    48721 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    48781 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    48841 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    48901 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    48961 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49021 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49081 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49141 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49201 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    49261 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    49321 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    49381 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    49441 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    49501 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    49561 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    49621 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    49681 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    49741 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    49801 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    49861 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    49921 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    49981 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50041 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50101 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50161 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    50221 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    50281 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    50341 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    50401 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    50461 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    50521 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    50581 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    50641 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    50701 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    50761 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    50821 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    50881 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    50941 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51001 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51061 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51121 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51181 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    51241 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    51301 gcagatgaaa aataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    51361 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    51421 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    51481 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    51541 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    51601 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    51661 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    51721 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    51781 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    51841 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    51901 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    51961 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52021 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52081 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52141 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52201 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    52261 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    52321 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    52381 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    52441 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    52501 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    52561 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    52621 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    52681 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    52741 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    52801 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    52861 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    52921 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    52981 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53041 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53101 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53161 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    53221 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    53281 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    53341 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    53401 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    53461 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    53521 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    53581 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    53641 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
    53701 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    53761 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
    53821 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
    53881 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
    53941 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
    54001 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
    54061 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
    54121 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
    54181 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
    54241 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
    54301 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
    54361 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
    54421 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
    54481 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
    54541 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
    54601 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
    54661 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
    54721 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
    54781 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
    54841 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
    54901 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
    54961 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
    55021 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
    55081 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
    55141 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
    55201 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
    55261 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
    55321 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
    55381 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
    55441 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
    55501 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
    55561 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
    55621 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
    55681 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
    55741 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
    55801 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
    55861 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
    55921 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
    55981 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
    56041 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta
    56101 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
    56161 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
    56221 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
    56281 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
    56341 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
    56401 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
    56461 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
    56521 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
    56581 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
    56641 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
    56701 ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga
    56761 attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac
    56821 ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag
    56881 tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa
    56941 gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag
    57001 cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa
    57061 aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa
    57121 actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc
    57181 ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat
    57241 caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa
    57301 aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat
    57361 tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg
    57421 tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc
    57481 ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg
    57541 tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac
    57601 ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa
    57661 gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag
    57721 gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct
    57781 tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa
    57841 aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga
    57901 aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag
    57961 attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata
    58021 attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac
    58081 attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa
    58141 aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt
    58201 gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc
    58261 caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca
    58321 cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt
    58381 cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga
    58441 acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga
    58501 gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt
    58561 agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc
    58621 agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc
    58681 tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga
    58741 ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca
    58801 agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg
    58861 tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt
    58921 ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc
    58981 gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc
    59041 accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat
    59101 ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg
    59161 gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga
    59221 gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa
    59281 atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat
    59341 cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca
    59401 aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct
    59461 ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct
    59521 tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc
    59581 aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac
    59641 agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa
    59701 catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt
    59761 catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga
    59821 atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc
    59881 tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc
    59941 ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc
    60001 tttgtcaatg gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa
    60061 acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac
    60121 ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac
    60181 ttattaaaac aatactttgg ttatgatgag tttagacctg gtcaaaaaga aattatccag
    60241 aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc
    60301 tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc
    60361 tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc
    60421 aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag
    60481 taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg
    60541 aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg
    60601 catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt
    60661 cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga
    60721 cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga
    60781 tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac
    60841 aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat
    60901 ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag
    60961 cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat
    61021 gcatttggta tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct
    61081 ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt
    61141 gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag
    61201 tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa
    61261 tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc
    61321 agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca
    61381 gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg
    61441 ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat
    61501 ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa
    61561 tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta
    61621 acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag
    61681 aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg
    61741 cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca
    61801 ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga
    61861 gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg
    61921 gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg
    61981 gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga
    62041 atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg
    62101 atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac
    62161 aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg
    62221 gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc
    62281 tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag
    62341 aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta
    62401 tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc
    62461 tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag
    62521 tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt
    62581 tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat
    62641 tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt
    62701 ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt
    62761 atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc
    62821 aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt
    62881 tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt
    62941 gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac
    63001 aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca
    63061 aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat
    63121 gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt
    63181 acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat
    63241 ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat
    63301 ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat
    63361 ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt
    63421 cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt
    63481 cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta
    63541 ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat
    63601 ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat
    63661 attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga
    63721 ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg
    63781 ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag
    63841 aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa
    63901 tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac
    63961 caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga
    64021 tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg
    64081 caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag
    64141 tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata
    64201 cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa
    64261 aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc
    64321 aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc
    64381 ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata
    64441 cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag
    64501 gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt
    64561 cagccattca atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag
    64621 ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg
    64681 cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc
    64741 atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa
    64801 tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc
    64861 aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc
    64921 gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt
    64981 ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc
    65041 taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg
    65101 ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag
    65161 acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac
    65221 gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat
    65281 ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt
    65341 ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt
    65401 ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg
    65461 atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag
    65521 ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg
    65581 atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac
    65641 aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag
    65701 cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc
    65761 atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca
    65821 tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct
    65881 tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga
    65941 aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga
    66001 atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac
    66061 agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc
    66121 atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg
    66181 gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag
    66241 gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac
    66301 ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga
    66361 atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg
    66421 cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc
    66481 aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca
    66541 caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg
    66601 gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg
    66661 tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca
    66721 tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa
    66781 cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg
    66841 atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg
    66901 gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga
    66961 ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt
    67021 ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc
    67081 aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa
    67141 ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct
    67201 ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag
    67261 atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt
    67321 cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat
    67381 gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca
    67441 tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc
    67501 ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc
    67561 agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga
    67621 aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa
    67681 agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata
    67741 caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag
    67801 gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc
    67861 tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca
    67921 aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt
    67981 tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc
    68041 tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga
    68101 aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc
    68161 aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt
    68221 caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag
    68281 catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag
    68341 ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata
    68401 gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt
    68461 ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat
    68521 gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg
    68581 ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg
    68641 cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa
    68701 gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga
    68761 ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa
    68821 ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct
    68881 cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc
    68941 caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat
    69001 aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc
    69061 aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga
    69121 gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg
    69181 ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa
    69241 catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc
    69301 aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata
    69361 tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa
    69421 aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg
    69481 attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga
    69541 gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac
    69601 ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc
    69661 aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct
    69721 gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca
    69781 gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa
    69841 tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc
    69901 ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac
    69961 tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag
    70021 cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat
    70081 gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac
    70141 gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt
    70201 ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat
    70261 gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga
    70321 atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc
    70381 gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt
    70441 agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga
    70501 cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg
    70561 acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct
    70621 gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga
    70681 gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg
    70741 attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa
    70801 gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat
    70861 cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga
    70921 tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc
    70981 aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca
    71041 agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg
    71101 acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac
    71161 aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt
    71221 cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga
    71281 acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg
    71341 tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt
    71401 tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga
    71461 gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac
    71521 accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg
    71581 gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc
    71641 aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag
    71701 ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc
    71761 gagcctccca aaataaatat caatacagcc actccatcag aacttcagca gattcctggg
    71821 atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag
    71881 aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa
    71941 aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg
    72001 ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt
    72061 ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt
    72121 ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa
    72181 cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt
    72241 atttattctc attgggacga atgatctagc agagcataaa caactttcta aagaaacgta
    72301 ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct
    72361 ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg
    72421 atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg
    72481 atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg
    72541 atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt
    72601 atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga
    72661 ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt
    72721 actgataagt cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta
    72781 ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa
    72841 aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat
    72901 gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca
    72961 ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat
    73021 tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat
    73081 ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct
    73141 gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga
    73201 aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac
    73261 gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc
    73321 cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt
    73381 gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa
    73441 aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt
    73501 gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa
    73561 cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc
    73621 tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc
    73681 ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac
    73741 catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt
    73801 ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa
    73861 agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt
    73921 cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg
    73981 tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt
    74041 ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt
    74101 gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg
    74161 gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt
    74221 catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc
    74281 tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc
    74341 tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc
    74401 tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgctgat
    74461 agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat
    74521 tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat
    74581 ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat
    74641 ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca
    74701 aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg
    74761 aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga
    74821 aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt
    74881 gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc attttactat
    74941 caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc
    75001 tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg
    75061 ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc
    75121 gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca
    75181 agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt
    75241 aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa
    75301 acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt
    75361 ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac
    75421 ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta
    75481 attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag
    75541 cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag
    75601 aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg
    75661 aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag
    75721 ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt
    75781 attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg
    75841 cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt
    75901 tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc
    75961 aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa
    76021 tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca
    76081 aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg
    76141 taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc
    76201 aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc
    76261 aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac
    76321 agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa
    76381 tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc
    76441 tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg
    76501 aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat
    76561 aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac
    76621 aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga
    76681 tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc
    76741 tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc
    76801 aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt
    76861 ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga
    76921 taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc
    76981 agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt
    77041 ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga
    77101 ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg
    77161 aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa
    77221 agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa
    77281 ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc
    77341 gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc atttgccact
    77401 tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc
    77461 tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat
    77521 tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt
    77581 cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt
    77641 tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc
    77701 agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct
    77761 aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc
    77821 gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa
    77881 tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat
    77941 gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta
    78001 aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa
    78061 atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc
    78121 ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag
    78181 aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag
    78241 ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg
    78301 ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc
    78361 cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag
    78421 ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta
    78481 aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt
    78541 ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt
    78601 tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc
    78661 tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct
    78721 tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc
    78781 aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat
    78841 cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta
    78901 gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc
    78961 tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac
    79021 tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt
    79081 tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc
    79141 ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa
    79201 gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat
    79261 taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat
    79321 catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac
    79381 tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg
    79441 taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag
    79501 tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc
    79561 aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa
    79621 ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc
    79681 attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc
    79741 acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca
    79801 acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac
    79861 ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc
    79921 ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat
    79981 cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc
    80041 tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa
    80101 aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag
    80161 ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg
    80221 ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa
    80281 tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa
    80341 acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca
    80401 caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata
    80461 aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa
    80521 aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc
    80581 ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga
    80641 acgataatgg ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt
    80701 attattaaag aaaataaaaa accagaacat ttctcaaata agag
//
LOCUS       DACDCV010000004        79284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDCV010000004 DACDCV010000000
VERSION     DACDCV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.004"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..1084
                     /locus_tag="IV041_000271"
     CDS             113..1084
                     /locus_tag="IV041_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8381092.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1116..2513
                     /locus_tag="IV041_000272"
     CDS             1116..2513
                     /locus_tag="IV041_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8381093.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2672..3295
                     /locus_tag="IV041_000273"
     CDS             2672..3295
                     /locus_tag="IV041_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381094.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            4044..6137
                     /locus_tag="IV041_000274"
     CDS             4044..6137
                     /locus_tag="IV041_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8381095.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            6297..7163
                     /locus_tag="IV041_000275"
     CDS             6297..7163
                     /locus_tag="IV041_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8381096.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(7185..7622)
                     /gene="ndk"
                     /locus_tag="IV041_000276"
     CDS             complement(7185..7622)
                     /gene="ndk"
                     /locus_tag="IV041_000276"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8381097.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            8281..10959
                     /gene="mgtA"
                     /locus_tag="IV041_000277"
     CDS             8281..10959
                     /gene="mgtA"
                     /locus_tag="IV041_000277"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8381098.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            11046..11717
                     /locus_tag="IV041_000278"
     CDS             11046..11717
                     /locus_tag="IV041_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8381099.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            12364..13437
                     /locus_tag="IV041_000279"
     CDS             12364..13437
                     /locus_tag="IV041_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381100.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            13439..14119
                     /locus_tag="IV041_000280"
     CDS             13439..14119
                     /locus_tag="IV041_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381101.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            14136..14975
                     /locus_tag="IV041_000281"
     CDS             14136..14975
                     /locus_tag="IV041_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8381102.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            15006..16229
                     /locus_tag="IV041_000282"
     CDS             15006..16229
                     /locus_tag="IV041_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8381103.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            16632..18050
                     /gene="nhaC"
                     /locus_tag="IV041_000283"
     CDS             16632..18050
                     /gene="nhaC"
                     /locus_tag="IV041_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8381104.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18554..18997)
                     /locus_tag="IV041_000284"
     CDS             complement(18554..18997)
                     /locus_tag="IV041_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8381105.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(19215..20555)
                     /locus_tag="IV041_000285"
     CDS             complement(19215..20555)
                     /locus_tag="IV041_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8381106.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20824..21720
                     /locus_tag="IV041_000286"
     CDS             20824..21720
                     /locus_tag="IV041_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8381107.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(21870..22811)
                     /locus_tag="IV041_000287"
     CDS             complement(21870..22811)
                     /locus_tag="IV041_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8381108.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            22937..24058
                     /locus_tag="IV041_000288"
     CDS             22937..24058
                     /locus_tag="IV041_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8381109.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24316..25818
                     /locus_tag="IV041_000289"
     CDS             24316..25818
                     /locus_tag="IV041_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8381110.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            25885..26412
                     /locus_tag="IV041_000290"
     CDS             25885..26412
                     /locus_tag="IV041_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8381111.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            26623..27684
                     /locus_tag="IV041_000291"
     CDS             26623..27684
                     /locus_tag="IV041_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8381112.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            27687..28583
                     /gene="murQ"
                     /locus_tag="IV041_000292"
     CDS             27687..28583
                     /gene="murQ"
                     /locus_tag="IV041_000292"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8381113.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            28597..30579
                     /locus_tag="IV041_000293"
     CDS             28597..30579
                     /locus_tag="IV041_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381114.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(30644..31090)
                     /gene="nrdI"
                     /locus_tag="IV041_000294"
     CDS             complement(30644..31090)
                     /gene="nrdI"
                     /locus_tag="IV041_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8381115.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            31301..32725
                     /locus_tag="IV041_000295"
     CDS             31301..32725
                     /locus_tag="IV041_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8381116.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(32906..33679)
                     /locus_tag="IV041_000296"
     CDS             complement(32906..33679)
                     /locus_tag="IV041_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8381117.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(33676..34581)
                     /locus_tag="IV041_000297"
     CDS             complement(33676..34581)
                     /locus_tag="IV041_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381118.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(34735..35055)
                     /locus_tag="IV041_000298"
     CDS             complement(34735..35055)
                     /locus_tag="IV041_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8381119.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            35529..36215
                     /locus_tag="IV041_000299"
     CDS             35529..36215
                     /locus_tag="IV041_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8381120.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            36217..37161
                     /locus_tag="IV041_000300"
     CDS             36217..37161
                     /locus_tag="IV041_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8381121.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            37360..38004
                     /gene="pcp"
                     /locus_tag="IV041_000301"
     CDS             37360..38004
                     /gene="pcp"
                     /locus_tag="IV041_000301"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8381122.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            38083..39360
                     /locus_tag="IV041_000302"
     CDS             38083..39360
                     /locus_tag="IV041_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381123.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(39544..40293)
                     /locus_tag="IV041_000303"
     CDS             complement(39544..40293)
                     /locus_tag="IV041_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8381124.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            40623..41003
                     /locus_tag="IV041_000304"
     CDS             40623..41003
                     /locus_tag="IV041_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8381125.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            41099..41608
                     /locus_tag="IV041_000305"
     CDS             41099..41608
                     /locus_tag="IV041_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381126.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            41685..41864
                     /locus_tag="IV041_000306"
     CDS             41685..41864
                     /locus_tag="IV041_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381127.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            41919..42482
                     /locus_tag="IV041_000307"
     CDS             41919..42482
                     /locus_tag="IV041_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381128.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(42529..43044)
                     /locus_tag="IV041_000308"
     CDS             complement(42529..43044)
                     /locus_tag="IV041_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381129.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43070..43939)
                     /locus_tag="IV041_000309"
     CDS             complement(43070..43939)
                     /locus_tag="IV041_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8381130.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            44065..44745
                     /locus_tag="IV041_000310"
     CDS             44065..44745
                     /locus_tag="IV041_000310"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8381131.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            44898..45878
                     /gene="pta"
                     /locus_tag="IV041_000311"
     CDS             44898..45878
                     /gene="pta"
                     /locus_tag="IV041_000311"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8381132.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            46001..46474
                     /gene="tsaE"
                     /locus_tag="IV041_000312"
     CDS             46001..46474
                     /gene="tsaE"
                     /locus_tag="IV041_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8381133.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            46467..46991
                     /locus_tag="IV041_000313"
     CDS             46467..46991
                     /locus_tag="IV041_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381134.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(47481..48014)
                     /locus_tag="IV041_000314"
     CDS             complement(47481..48014)
                     /locus_tag="IV041_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8381135.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(48878..49630)
                     /gene="xth"
                     /locus_tag="IV041_000315"
     CDS             complement(48878..49630)
                     /gene="xth"
                     /locus_tag="IV041_000315"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8381136.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            49743..50663
                     /locus_tag="IV041_000316"
     CDS             49743..50663
                     /locus_tag="IV041_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8381137.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            50736..51671
                     /gene="murB"
                     /locus_tag="IV041_000317"
     CDS             50736..51671
                     /gene="murB"
                     /locus_tag="IV041_000317"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8381138.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            51969..53447
                     /locus_tag="IV041_000318"
     CDS             51969..53447
                     /locus_tag="IV041_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8381139.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            53724..55784
                     /locus_tag="IV041_000319"
     CDS             53724..55784
                     /locus_tag="IV041_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381140.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            55867..56436
                     /locus_tag="IV041_000320"
     CDS             55867..56436
                     /locus_tag="IV041_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381141.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(56611..57417)
                     /locus_tag="IV041_000321"
     CDS             complement(56611..57417)
                     /locus_tag="IV041_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381142.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            57555..59069
                     /locus_tag="IV041_000322"
     CDS             57555..59069
                     /locus_tag="IV041_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8381143.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(59472..60455)
                     /locus_tag="IV041_000323"
     CDS             complement(59472..60455)
                     /locus_tag="IV041_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8381144.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(60460..61107)
                     /locus_tag="IV041_000324"
     CDS             complement(60460..61107)
                     /locus_tag="IV041_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8381145.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(61192..61824)
                     /locus_tag="IV041_000325"
     CDS             complement(61192..61824)
                     /locus_tag="IV041_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8381146.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(61840..62391)
                     /gene="coaC"
                     /locus_tag="IV041_000326"
     CDS             complement(61840..62391)
                     /gene="coaC"
                     /locus_tag="IV041_000326"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8381147.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(62388..63125)
                     /locus_tag="IV041_000327"
     CDS             complement(62388..63125)
                     /locus_tag="IV041_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8381148.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(63122..64111)
                     /locus_tag="IV041_000328"
     CDS             complement(63122..64111)
                     /locus_tag="IV041_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8381149.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            64369..64911
                     /locus_tag="IV041_000329"
     CDS             64369..64911
                     /locus_tag="IV041_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8381150.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            64928..66013
                     /locus_tag="IV041_000330"
     CDS             64928..66013
                     /locus_tag="IV041_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381151.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            66025..66831
                     /locus_tag="IV041_000331"
     CDS             66025..66831
                     /locus_tag="IV041_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381152.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            66832..67659
                     /locus_tag="IV041_000332"
     CDS             66832..67659
                     /locus_tag="IV041_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381153.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            67656..68729
                     /locus_tag="IV041_000333"
     CDS             67656..68729
                     /locus_tag="IV041_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8381154.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            69157..71853
                     /locus_tag="IV041_000334"
     CDS             69157..71853
                     /locus_tag="IV041_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8381155.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            71930..72418
                     /locus_tag="IV041_000335"
     CDS             71930..72418
                     /locus_tag="IV041_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8381156.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            72430..72924
                     /locus_tag="IV041_000336"
     CDS             72430..72924
                     /locus_tag="IV041_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8381157.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            72945..73754
                     /locus_tag="IV041_000337"
     CDS             72945..73754
                     /locus_tag="IV041_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8381158.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            73751..74572
                     /locus_tag="IV041_000338"
     CDS             73751..74572
                     /locus_tag="IV041_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8381159.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            74623..76722
                     /locus_tag="IV041_000339"
     CDS             74623..76722
                     /locus_tag="IV041_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8381160.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            76719..77837
                     /locus_tag="IV041_000340"
     CDS             76719..77837
                     /locus_tag="IV041_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8381161.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            77876..78754
                     /locus_tag="IV041_000341"
     CDS             77876..78754
                     /locus_tag="IV041_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8381162.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            78945..79097
                     /locus_tag="IV041_000342"
     CDS             78945..79097
                     /locus_tag="IV041_000342"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381163.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        25769 a        13813 c        16550 g        23152 t
ORIGIN      
        1 agactttagt tatatggata ttgatgacaa gcaacactat gcaaagaatg cggtcatcat
       61 aatcgaacaa caagaaagag aaatcaaatg taactggcag gagtgagaat gtatggcatc
      121 gatccgtgat gtggctaaga tggctggtgt ttcagtggga acagtttccc gttacttgaa
      181 tggtcagcaa ctgaaggaaa agaacatgaa aaaaatagca gaagcgattt cagcgttgga
      241 ctataaagaa aacatcattg ctaaagggtt gaagaataat cgaagcttct ctattggact
      301 tttgataaac gggatttcca gccgttttgg gtcggaagtt gtctcaggga tcgaaagagt
      361 agctgaagaa aatgggtata gcttattatt aagcgggttt gccgatcagc cagaaaaaat
      421 tgaacaaaaa attgaatacc tgatgaagca tgtgatcgat gggctgattg tcttcttatc
      481 cgaagaagag tggtctggat ttgaaatgct cacaaaaatg agtattccag ttatcgcatt
      541 gaattgtcca gaaggaccag caggtattga tacaatatta gtgaatgatc gagaaagtgt
      601 agcaaaagtg atcgctcatc atggtgaaca aggacataaa aaaatcggat ttattgctgc
      661 cactcaaaca gactacgtag caagagaacg tttagcagga gccaaagaag cggtccaaaa
      721 tgatccatcg atccaattag aggtctttac aggagactat tctagaaaaa gcggctatta
      781 cggggcaaaa gcattattag aaaaaggggc aactgctatt tttgtcagca attacaatat
      841 gtcgattggt gccatcgaat gtttcaatca agaaggtgtc agaatcggga aggatattgc
      901 gttcagtcac tatgattatt tagataaaaa cgatctgagt atccttccga aaatcacgat
      961 cacgccacca acagataaaa tcggggaacg agcagcagaa cagctgttaa aacgaatcaa
     1021 aaaagaagag gaagaaaatg aaaaagtgat tgtgatggaa aatatcatac atggaatcaa
     1081 ctaaaacgaa ctaaaaaaga aaaagaggtg ctttgatgaa aaaatatcaa tttccagcaa
     1141 atttttggtg gggttctgca gcaagtggcc cacaaacaga aggaaggatt tcaggcgatg
     1201 gaaaaggaga gaacattttt gattattggt acaaaaaaga accgcaaaag ttctttgatc
     1261 aagtaggacc tgaaaaaacc tcgcaagttt atacgaagta tcaagaggat gtacagttga
     1321 tgaaacagac tgggcataat tcatttcgaa catctatcca atggagccgt ttgatacctg
     1381 atgaatctgg aacaatcaac gaagaagccg ttcgtttcta tcatcgttat tttgatgagt
     1441 tgattgaaaa cggtatcgaa ccctttgtga atctgtatca tttcgatatg ccgatgtatc
     1501 tgcaagaaaa aggcggatgg ttgaaccgag aaacggttga agcctatgaa aaatatgcca
     1561 aaagttgttt tgaattgttt ggttcgaaag taaaaaaatg gtttactcat aatgaaccaa
     1621 tcgttccagt cgaagcaggt tatttatatc gatggcatta tccagaagag tcagatatga
     1681 aaaaagcgat ccaagttggc tatcatgaag cgttggcttc agcgttagcc atcaaatctt
     1741 accatgaaat ggagcaaggt ggtgttattg gtatcatctt gaatctgaca ccaagctatc
     1801 caagagacga atcaaaccct gaagatgtta aagcagcgca aatagcagat gcgttcttca
     1861 atcgttcatt ccttgatcca gctgtaaaag gcgagtttcc aagtgaattg attgagatag
     1921 taaaagaact tgatctcatg cctgtgatcc atgcagagga tttgacggtt atcaaagaaa
     1981 atacagtaga tcttttaggt atcaattatt accagccacg ccgggtaaaa gcaaaagaaa
     2041 caccaatcga tactaaaaac ggtccaatgc ctgaggatta tttcgacaat tatgaaatgc
     2101 ctggtcgtaa aatgaatcct tatcgcggat gggagatcta tgaacaagga atctatgata
     2161 ttttgatcaa tgtacgagat cattatggaa atatcgactg ctttatttct gaaaacggta
     2221 tgggtgtaga aaacgaagca cgatttatca aagaagatgg catgatccac gatgattatc
     2281 ggatcgaatt tgtccaatct catctgaaat atgtccatca agccattcaa gaaggtgcca
     2341 attgtttagg ctatcatatg tggacatgta tggataactg gtcgtggacc aatgcatata
     2401 agaatcgata tggctttatt tcagtagact tagcacaaga tggaaaaaga acaatcaaaa
     2461 aatcgggtca ttggttcaaa gacgtatcag ataaaaacgg atttgaggct taagaaagaa
     2521 tagttgagag caaatgcgtg aaattctcca ttcttaccat tttgtgaaaa agaagttcaa
     2581 ttatttacga tctttttcaa cttttttgtc tttctatttc attttttgta tataatagaa
     2641 tagtcaaaat taaataggag tggaggaaag catgaggaaa aaaagaccgt ttcttctctt
     2701 ttggttattc ggttttttct tactgcttat tttatccgca gcattttcag gaaaaatgac
     2761 agcagatctt gctcagaaag aaacagaaaa aatcgtagca agcaaagaat cagtagataa
     2821 tttcaaaaaa gaattctcaa cttattggtg ggcggagtat aaaataactg ccaattacga
     2881 aaaaggcgaa ttggacattt atttgccaaa aggaaaagaa gaaaatcaat ttattaatat
     2941 cgatgacgta cttagtgcga tttctctcag acaatatcaa tttaaagaca agcatttgac
     3001 cgttttgctt ttgactcatg atggcgacaa agtagcccgt ttgaaaaatg gaaaagtgat
     3061 caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc gatcgtttag cggaatggaa
     3121 aaagacaaat gaacaagtca agaaaacaac tgtctcttct caaaaaaacg agtcgacagg
     3181 aacaagtcct tcagattctg gcagtggaca aacgataacg ggaagtgaag aaaatcaagc
     3241 ctcaattatt acgaatgagt catcacaagg acaatatcaa tcctatggac aatgaaaaac
     3301 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc
     3361 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtgtatc
     3421 aagaggtctt gagcctgccg tttaactctg agccacaagg agcaatttca aaaaatagct
     3481 ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3541 accaagaggt tgtaaaatta gcgtcctgac ataatctaat accgagggtc agctgattga
     3601 acaccgtgta tcaagaggtc ttgagcctgc cgtttaactc tgagccacaa ggaacaattt
     3661 caaaaaacag cttctcatgt tttataaata ttgtgacttg tggccgagga gttggcagga
     3721 gaacaccgtt taaggaagaa gttgtgaaaa agctgtcctg catcaagaaa taaggagctg
     3781 cgacaagaat tttgtcacag cctctttttt tgtacgaata ccgagaaact ggcttttgaa
     3841 taccgtggag tgaagcggtc accacaagac ttttatctct tcattagttt aatcgcttac
     3901 atcgcagcgg ttttatgctg tgaaaagtgg acggaataat ctttagaaag aataaaaaaa
     3961 tcttaagata acaacttttt taggaaattt tcatttttca ggagcaaaat aagaaaaaat
     4021 gtgaatagag agaagtgata tacgtgacac aacttaaaaa attgtgccaa aatcgtttag
     4081 caatcgtttt gacagctgtt ttctttttct ggctgaagac gatcattgcc tactatgcag
     4141 atttttcatt aggtgtggaa ggtacgatcc agtactttat cttgtggatc aacccaattg
     4201 cgactacctt attgtttttt ggcctttctt tgtatattaa aaagccaaag ccagcgttag
     4261 ctattctttt gatcattgat attttaaata cgcttttgtt gtacttgaat attatttttt
     4321 atcgggaatt cacagatttc attacagtca aatctgtatt aggcttttcg aaagtgtcgc
     4381 aaggattgtc aggaagctcc ttttctttga tgaagcccca tgatgtcatc tactggctgg
     4441 atatcgctgt gttcattggg ctactggttt ggttgaaggt caagaaaata cctatcaaaa
     4501 gcaaccctgt cggcaaaccg atggctatcg ctgttacctg tctttcaggg cttatttttt
     4561 caggaaatct agcgctgagc gaagccaacc gtccacaatt gctgcagcga acatttgacc
     4621 gttcatatat tgtcaaatat ctaggaatcg atgcgtatac tatttatgac ggaatcaaaa
     4681 caggaatgac tagcagtgtt cgcgcgcatg caagcagtaa tgggattgat gaagttttag
     4741 attatacaaa gaaacattat gcagagccaa atcccgaaac atttgggatc gctaaaggga
     4801 aaaatgtgat cgttctccat ttagaaagtt tccagcagtt tttgattaat atgaaggtag
     4861 acggacaaga agtcacaccg tttttaaata gtattttcca gaatcaagca acgatcagct
     4921 ttgacaattt ctttcatgaa gtaggacagg gaaagacaag tgatgctgaa aatatgttgg
     4981 aaactggcac atttggttta cctcaaggct cattattcac agaacttggg tcagacaatg
     5041 tctttcaagc cgcaccagcg atccttggac aaaagcaagg ctatacaagc gctgttttcc
     5101 atgggaatgt agctagtttt tggaatagag atcatgtata taaaaactta ggttatgaca
     5161 atttctttga tcgatcttat tttgatgaat ccgacgaaac gttaggttat gggatattag
     5221 ataaagattt attcagagaa tctgcccaat atcttgagca tctacaacag ccgttttaca
     5281 cgaaatttct ttccgtaacc aaccacacgc cttattatac ggatgacaag aatttcgact
     5341 ttccatcttt gaatactggc aatagcacag tagataatta tgttcgaacg gcacattatc
     5401 tggatcaatc attggaacaa ttttttacgt atttgaaaaa atctggtatt taccaaaatt
     5461 caatctttgt gatttacggg gatcattttg ggatctcgaa tacagataat aaagatttag
     5521 caagcgctct tggtaaagat ccagatacat gggatgaatt tgacaacgca cagatgcaac
     5581 gagtgccatt gatgatccat atgcctggtt atactaaagg gacagtcaat catgaatacg
     5641 gtggagaaat cgatgttttg ccgacattac tacatctatt gggaatcgat gataaagatt
     5701 atcttcattt tggtacagac ttattatctt ctcagcatga tcaagtagtt gcttttcgaa
     5761 atgggaactt tgtgacaccc aaatacaccg tcctaggagg aaaagcttac aataatcaga
     5821 caggagaaat catcgatacg aaagcagcag gaatcgatga ggaaatctcc aaagatcagg
     5881 aaaaagtcaa gactgcgtta agtctttcag ataagctgaa tcaggaaaat ctcttgcggt
     5941 tttatgtgcc aaacggattt gagacgattg atccgaaaaa atatgactat aaaaatgaag
     6001 cggaacgtaa tgaagcaatc gaaaagcaaa aaggcgaaca ttcgacaagt gtcttttcaa
     6061 aaaatggtaa taaaacaaca accaacttat atccagtaac aagaacagac gttgaacagc
     6121 aaaaaaataa tcaataaaca atcaaaccaa ccttcagtag ttagacaaca ttctaatgct
     6181 tgaaggttgg tattttttat gaaaaaaaca tttttttatt tttttcgtgt atttttcctt
     6241 tttaacgtat ctatttattg aatgtggaaa aatttaaatt aaaaaggaga actattatgt
     6301 atgatggtga attgatcaag aaacttcgag tcaacaagaa gttgacacag tcccaattag
     6361 cggaaggaat ctgttttaag acttctctag tcgggattga gacaaattca gtgaaaaaaa
     6421 tgtcatttgt aacattgaga tcatttttag agcgaatgaa catgacactt gcagaatatg
     6481 aatggatgcg aaaccagctg gatgaaccaa taaagttaaa gaaaaaaaga cgaatgctag
     6541 atcgggtaca agaagacagc tttgatcctt ataaagagat cagcaataac cgtaggcatt
     6601 ttaagaaaac agaagatctg tattatttgg ttttgaatct tcacatggtc gaaaaaacag
     6661 aagtatctga gacagaattt gaatttttac gttatgaatg ccgaatcatt gaagaatact
     6721 tgatgcagat accagactat ggtcaatttg aactatctat tctagctgat tttccttgga
     6781 tattcagtga tcggttcatt gagaacaatt atttgaaaat caaaaaaaga atgcgacgat
     6841 tagcacagtt gaacaatcat gagcagtatc tctttacttt tttaatgaac ctgacttcgt
     6901 tttatataga aaatggtcgg ttgaaaaagg cacgtagtgt gaatgaagat atgaaacgga
     6961 gcttagcaca taaagaaaga agtacagtga tctatgaaac attgatggca gattattatc
     7021 aaagattgat ttctgctgct ttaggggaag aaaagacgaa agactaccaa gaattcttca
     7081 cagtgctcga atatatgtta ggaaagaatc aacggaggtc cttagagaaa aaactgctgg
     7141 ccattgctga acccagcaac tagaccagca gtttagaatt tgtatcactt cgccgctttt
     7201 tcagacggtt tgtttatatc gaatggcggg aaaaagcggg cgatctcttt taccgcagca
     7261 tcacgggaat cagaagcgtg aatgatgttt tcagtccccg gcagcgcgta gtctcctcgg
     7321 atcgtacctg gaacagcatc ggcagctttt gttgctccaa ccatcttacg gacgatatcg
     7381 atcacttcct cgccgaccag cacaagataa acgacaggac cggaagtcat gtaatcgatc
     7441 agttcatcaa aaaaactgcg ctcagtcaag tgctgataat gttcttttgc taattcagga
     7501 gtcatagttt caaacttcat ttcagcaatg gccagtccct ttgcttcaaa acgttggatg
     7561 atgcttccga ctaaatggcg tctcacgccg tctggcttga taataacaag tgttctttcc
     7621 ataacagcac ctcgtttcct actatatttc tattatagcg ggaggtggaa aatgagacaa
     7681 ataaaaccgc gtgagacagg aatataacgt gtgtatttta aaaaaagtat gaaaaacatg
     7741 gaaaattgat ttgacatcct cttttttagt tgttatatta acgaaggaaa accgaataag
     7801 atttgttagg tgaggctcct atacaaacat aggctactgc ccaaaaacgt cgagagacgc
     7861 caatgggtag aacaggaatt gtcgagataa ggcttttcat aaggtagcta aaagcaggta
     7921 tctgcttttt acgttgtaca gtgtcaaaac tcaacgatga gatctgagaa gcttttttta
     7981 atatgctttt tcaaaccttt cgctgagtat ttggcgagag gtttttttgt tgccgcaaat
     8041 ccccatcggt gaaaaataaa aaggaggtgg tctaattgga aaaataacta ttcgaaaaat
     8101 gattggaagt catgaattaa aattacttag taaaggagat gagaacaaat ggatgacagt
     8161 cattcgaaaa acaaggatct agaggaaagg ccagggctat tatctatctg ttctcagccc
     8221 ctttcagttt tttctctatc gatccgatga atagctaaag accaaaagca gcgagaacag
     8281 ctgtcttttg gacacaaagg agagattgag atggaaaaaa agaaagatac acaaaaaatc
     8341 tatgcagctt ttgctggaaa aacgaaacaa gaattatttg aactattcca tgcatcagca
     8401 aatggattga cagaagaaca aatcgaacgt tcaagagaaa attacggtga aaatgcgatt
     8461 tcatatggca aaaaaacacc attcattgta gaaatattga aagcatacat cacgccattt
     8521 acattagtgt tgatcgcatt aggaattatt tcattcatca cggaatatgt attagcagct
     8581 cctggagata aagatctttt cggtgtagcc attatcttca ctatggttat cgtcagcggg
     8641 acaatgacgt tgattcagtc cgtacgttcc aatcaagcgg ctgaaaaact gaaatcactt
     8701 gtgaaagtaa cggcagctgt aaaacgtaat ggaaagtaca cagagattcc tatggaagaa
     8761 atcgtctgtg gagatcttgt tcggctttca gcaggggata tgataccagc ggatatgcgt
     8821 ttattgtccg caaaggattt attcatttcc caagcagcat tgacagggga aagctatcca
     8881 gtagaaaagc atgcgaaagt atgtgaagac gatcagcaaa gcgaaacaag ttacgaaaat
     8941 cttgtcttta tgggaagtaa tgtcatcagt ggaagtgcgg aaggaatcat tgtttctgtt
     9001 ggaaatgaaa cactatttgg tcatgtagca gaaacgctat ctgaaaaacc aattcaatca
     9061 agctttgaat taggcatcca taaaacatcc atgctactga tcaaatttat gtcattgatg
     9121 gctccagcag ttatcgtcat caatgggctt acaaaagggg attggctaga agcttttctt
     9181 tttggactat ctgtagcagt tggtctaaca cctgaaatgc ttccaatgat cgttacgacg
     9241 aatttggtca aaggagcaag tgtgatggcc aaaaaaggaa cagtgatcaa aaatttgaat
     9301 gccatccaga actttggtgc aatcgatgtg ttatgtacgg ataaaacggg gacattgaca
     9361 caagataaaa tcattcttga atatcatatg gattgtgaag gaaatgaaga tgatcgtgtt
     9421 ttgcgccatg cgtatctaaa tagctactat caaacagggc tgaaaaattt gttagatgtg
     9481 gctatcattg atgaagcaac agaaacttta gatacagata agatcaatta tcaaaaagtg
     9541 gatgaaattc cttttgattt tgaacgtcgt cgtatgagtg ttgtggttgc tgatccagct
     9601 ggtaaaacac aaatgatcac aaaaggcgcg attgaagaaa tgctgaacat ttctaaattt
     9661 attgatcttg gcggtactgt tacaccatta acaaaagaac gaaaagaagc cgtacttgca
     9721 aaagtacagt cacttaatga agatggctta cgagtgattg gtgtcgcaca aaagaccaat
     9781 ccaagcgtcg ttggagaatt ttcagtcaaa gatgagtcag acatggtttt gattggttat
     9841 cttgctttcc ttgatccacc aaaagaaaca acgaaacaag cgttgaaagc tctgaaagaa
     9901 catggtgtag ctgtaaaagt tctgactggg gacaacgaat tagttacacg ttcagtgtgc
     9961 cgtcaagtag gtctagagat caatgaattg gttaccggtg agaaaatctc ggaaatgagt
    10021 gatcatgaat tagcacaagt agctgaagac catgaagtat tcgtaaaatt gaatcctcag
    10081 caaaaagcac gtttgacgac agctttacgt aaaaatggtc atactgtcgg atttttagga
    10141 gatggtatca atgacgctcc tgcaatgaaa gtggctgata tcggtatttc tgtagataca
    10201 gctgtggata ttgcgaaaga atctgcagac gtgatccttt tagaaaaaga tttgacgatt
    10261 ttggaaagag gccttctttc tggacgagaa acttttggga atatcatgaa atatatcaag
    10321 gctacagcaa gttctaattt tggcaatatg ttttccgtgc tgattgcaag tacgttcctg
    10381 ccatttttac caatgctgcc tttacaactt ttgtttttga atctgatcta tgatgtttcc
    10441 tgcatttcct tgccatggga tcggatggat aaagaatatt tggacgagcc aaaaaaatgg
    10501 aatgcatcta gtattgggga attcatggtt tactttggtc caacaagttc gatatttgat
    10561 attactactt acttgttgat gtattttgtt atttgtcctg ctgtagtggg cggaagtttc
    10621 catacattgg atagttcaca gcgagtgatt ttcatcgcat tattccatgc aggatggttt
    10681 gtcgaatcac tatggacaca aaccttagtg ctccatgcac tgcgtacacc gaaagtacca
    10741 tttatccaaa gcaacgcaac atttgccatg tttgttatca ctacattagg aattattgtc
    10801 ggttctgtcc ttccttttac aagttttggt gctgaattag gcttgatgcc acttcctggc
    10861 aattactggt tatggttatt tgctacagtc atcgcttatc tggcgcttgt gacagctgtg
    10921 aagaaaatct atatccgtcg attccaagaa ctcttataaa cgagaaggct ggttctttgc
    10981 ttctgttctt tttggaccag tcgtgctaaa ctatgtgtat tgttttggaa aggattaatg
    11041 tatagatgaa aggtgcaata tttgatttag atggtttgtt agttgacaca gaaaaaacgt
    11101 atcgtgatgg ctggctttgg ggatttcagc aatttgatat ccatatccca aaagaaatcg
    11161 ttgacgcttg gggcggaaaa aactggaaac aaagttttga tattttagtg aaagcagcag
    11221 gtagccctga aaaagtacag gaagtccgag cgaagcgtga agaatatttt tatcaacagc
    11281 tgacaagcgg agccatccac ttaaaaccat atgcaaaaga aatattggca gacttaaaag
    11341 aacgtcatat gaaactaggc attgctacga ccactgttac gaaaagagca acagatatct
    11401 tggatcactt taaattagct gattacttgg acacgtttac attcggagac gaagtgtcgg
    11461 agaacaagcc ttcaccgatt ccttatctga cagctttgga acgaacagga ttagaagcta
    11521 gttcagcctt tgcagtggaa gattctttag taggagcaac agctgcttca cgagcaggat
    11581 taggcgttgt tttgatacct gattccagtt ttgtccgaaa ttataccgtc gaagaaaagg
    11641 aaaaattgaa acttcttgcg gaaggaaaag atcttcgaac tgttattgaa atgttaaata
    11701 aaaaaacaga aaaataaata tagaaaaaat aatttgaatt tcgtacttga cttttcagtt
    11761 ttcctcatgt agattaatcc caacaacaaa atatcgattt cgttgaaaag gaaagtaagt
    11821 ttggaaactt tgcagagagg ctctggctgg tgagaagagt caagtaagaa aagctgaaaa
    11881 tggccttgga gaataatttg tcgataagta ggcaaattcg gaactgttcg accgttaaaa
    11941 cgaacgccaa tctctctttt tgagatgttg ggcttgaggg aaggaaactt ccgaaaaaag
    12001 gtggtaccgc gatacgtcgt ccttttactg acatagttta tgttagtaaa aggacttttt
    12061 ttttgaatat tatttgcatt gaaatggtaa gtacgataga agaacctttg acagagagct
    12121 tcggtagctg agaagaagca aggaacgact attggaaaat ggccatcgag caattcatcc
    12181 cgagcataga tgtaaggcga tgacggctgc gaccgatatc ttgctagagt atgatcgtac
    12241 ttgaagagga gttgtgtgtg aacacagctt gaacatgagg tggtaacacg caaaaaagcg
    12301 tcctcaagaa aatgacattt tcattttctt gaggacgctt tttttagata aggaggaatt
    12361 ttaatgattg aattgcgaga tgtttctgta tgtttcaaac aaaaagaaaa agagatacag
    12421 gcagtagatt ctgtagattt aacgattgaa aaaggcgatg tttatggaat cgtcggttac
    12481 tcaggtgcag ggaaaagtac gttagttcgt gtgatgaatt tgctgcagaa accaacagca
    12541 ggagaagtca ttgtcaatca gacgaatctg agtcagctct ctcccaaaga attgcgaaaa
    12601 gaaagaaagg ccatcggtat gatctttcag catttcaatc taatggaaag ccgaactatt
    12661 tttgacaacg ttgatttttc tttaaagtat tctggaaaat cgaaacagga acgtcgtcaa
    12721 aaagtgaatg aactactgga acttgtcggt ttggaagaaa aagcatctgc ttatcctaaa
    12781 caactgtcgg ggggacaaaa acaacgagta gcaatcgcac gagctttggc gaatgaacct
    12841 aaggtattat tatgtgatga agcaaccagt gcacttgatc cgaaaacgac ccttcagatc
    12901 cttgcattgt taaaaaaatt gaatcgccag ttaggtttaa caatcgtcct gatcactcat
    12961 gagatgcaag ttgtcaaaga aatctgcaac aaggtagcgg ttatggaaga tggaagaatc
    13021 gttgaaaaag gtagcagcat ccagatcttc agcaatcctg aagaagaatt gaccaaagat
    13081 tttattcgaa cagctactca tcttgaccaa gcattagaaa cgatcattgc ccattctgct
    13141 tttgccgatc agatcgccaa taaatggctg gtggaattaa gttatattgg aaatcagacg
    13201 aacgaacctc taatcgctca tctgtacagc aaatatcaag taacagcgaa tattttatat
    13261 ggcaatgtag agttattaca agaaacgccg ttagggagtt tgatcgttac tttagctgga
    13321 gaaaccaaac agcggaaaaa agcattggat tatttgatca aatctggtgt aaaaataaat
    13381 attttacaaa aaaacgattt agatagtcgt atcaaagtga tcgaaggagg aagataaaat
    13441 gactgatttc tttaaaacat atttgcccaa tgtttatttg attccggatg aatttgtcga
    13501 agcgacgaag caaacaatat atatgtcttt ttggacagca ttcattggcg ggatgattgg
    13561 aatcatctta ggtgtgacgt tagtagtgac acgccctaat ggattactag ctaaccgtct
    13621 gctatttgaa atacttgata agctgatcaa tatcattcgt tccatccctt tcattatctt
    13681 gctttctctt ttagcattga ctactcgttt cttagttgga acgacgattg gtgcgaaagc
    13741 agctttagtg ccattgattt ttggagttgt tccgtttttt gctcgtcaaa tcgagaacgc
    13801 actgctagaa gttgatcctg gggtgatcga agcggcagaa gcgatgggaa ccagtccaat
    13861 cggtatcatt ttacgtgtgt atttgttaga aggtttgcct ggtatcattc gggtctcggc
    13921 gttgactatc atcaacgtga tcggactgac taccatggct ggagcagtag gtgcaggcgg
    13981 attagggaat cttgctatat cgagagggta taatcgattc caaacagatg taacgattgt
    14041 ggctacatta ttgattctaa tcttagtttt tttaagtcaa tttatcagta atcaactgat
    14101 taaaaaaatt acacattgaa agtggaggag aaaaaatgaa aaaaattatt cgttttgtag
    14161 tcattacatt tgtagcaatt atttggttgg cagggtgtag ccaaggaaaa gcagatacaa
    14221 ctgacaaaaa agaaactatc gtaaaactag ggattattgg agaagataca gatgtatggg
    14281 acaacgtgaa agaccgtttg aaaaaggaga acattaattt ggaatacgtg aaatttacag
    14341 attacaatca accaaatgtg gctttagcag atggatcgat tgatttgaat tctttccaac
    14401 atcaattctt tttagacaat tacaataaag aacacggaac tgatttagta tcgatcggaa
    14461 atactgtcaa tgcaccgctg ggaatttatt ctaacacttt gaaggatgta gctgatatca
    14521 aagacggagc aaaagtagca atccctaatg atgttacaaa tggcggcaga gctcttctgt
    14581 tattgcaaac tgctggattg atcaaagtgg ataaggcaaa aggacaagcg cccactgttt
    14641 ctgatattac cgagaataga aaaaatctag acatttctga attagatgct tcccaaacag
    14701 cacgtgcttt gtcagatgta gacatttcag tgatcaatag cggtgtagct gtagatgctg
    14761 gatttaatcc tgcttctgat gccatcttct tagaaccagt ggatgaccat gcacgtcctt
    14821 atgtgaatat catcgttgca agaaaagaag atgaaaacaa taaaacgtat caaaaaatag
    14881 tagacgctta tcaaacagaa gacacagtca aagtaatcga gaaaacatca aaaggatcaa
    14941 gcgtgcctgc atgggaaaca ttcgggagaa aataagaaaa atgactagca taggaggaat
    15001 tttttatgac aacaacaatc tcaacaaacc aattgaagga tctaatccaa tatcgtttac
    15061 cagaatatca agcacttgct ctagatatcc acgaacatcc tgaagtaagt aactatgagt
    15121 tttattcctc agcagcgttg attaaacaat tagaaaaaga aaggttttgg gtagaaaaag
    15181 aagtagctgg acaccgaaca gcctttgatg cacgttatat ttccaataaa cccggtccca
    15241 caattgcctt tttggcagaa ttcgatgctt taccaggtat tggtcatgca tgcgggcata
    15301 atttgtttgg tacttactcc gtattagccg ccagcatagt gaagcaattc attgatgaaa
    15361 ctggtggaga aatccgtgta tatggcaccc caggggaaga agggggcgag aacggatcag
    15421 ctaaaggcag ctttgttcga gaaggttttt ttgaggatgt cgatgtagcg ttatgcgtcc
    15481 atccagcttt tcgttatggg aaaacgacag aatctttagc gaatgatcca gtcgacatca
    15541 aattttacgg tgtcgcttcc catgcagcag cagcacctga aaaaggagta aatgctttgg
    15601 aagcattgat ccaagtgttt aatggaatca acgctctacg cttgcaattg ccaaaagatg
    15661 tcaatatcca tgggatcatt acagacggag gagtagctgc caacgtcatt ccagattacg
    15721 ctgctggacg tttctattta cgtgcaggaa atcggcaaac attagataaa gtatatgaaa
    15781 aagtggaaaa tatcgtacga ggtgctgcga taagtacggg cacgactttt gagtttggat
    15841 tgttccaaaa tgcagttgat gatgtcatcg ttactccttc ttttgatgac ttattcttcc
    15901 agcatgcaaa agaagcaggt gttccagatg aagaaataga aacggaacaa agaacaagtc
    15961 tcggttcttc tgatgtagga aatgtcagcc aagtcatacc aacaatccag cctacggtag
    16021 caatctctga gacatatatt gccggacaca cagaagaatt caaagctgct gctaaaagtg
    16081 acaaaggcct tgcttctatt gcgattgcag cagaactact cgccaataca gctcttgatc
    16141 tgttgaaaga gccaaagcta ttagaaagaa tcaagcaaga acatcaagaa atagtagcaa
    16201 gaaaaaaaca agagggcgct actttctagt aaaaaataaa cttgtgtgtg aaagttataa
    16261 aaataatttt cagaaaaatg aaaaaatatt ttctttttca ttgacaaatg atcttatgga
    16321 aggtaaactt cgtacaagtc aacttttcac gcagtagcgg ttaggtgttt tgttcagaaa
    16381 gtcggtggtc gctgtgaacc gatcaagcct aattgacgaa ttacatggaa agtccataca
    16441 tttgaataaa agtttgaaag tgaaaaacag atctgttttt aattaaggtg gtaccgcgga
    16501 gtcattcgtc cttagtttaa aacaggtctt tttattttta taaaggagga atcgcggatg
    16561 aattgcggcg gagtaatccg atatcttaac ttttttagaa cataagaaca actaaaaaag
    16621 gagagattat catgggaaaa aaaggaacac caagtttttt agaagcaaca cttgtattaa
    16681 tggttattgt tttaggcatt gcgattggcg ttgtcggatt aaaggtctca cctaatatca
    16741 cgatcttagc agcgatcgga atggtcatgt tgtatgcagt gataaaaaga tacccgacag
    16801 attggctgca cgaaggaatc atcaatggca tcaaaccagg aatcatccca atctttattt
    16861 ttattttagt cggttcattg attgctgtat ggatacaagc agggatcatt ccaactctaa
    16921 tggtcattgg gttcaaattg atcagtgtcc agtggtttgt cccttctgta tttgttgtgt
    16981 gtgcaatcgt aggtagcgct gtagggagtg cctttactgt tatgtcaacg attgggattg
    17041 catttttcgg tattgggaca acactaggct taaatccagc gctggttgtt ggagctattg
    17101 tttcaggtgc cgtttttgga gataagatgt ctccattatc cgaatcaaca aatctagcag
    17161 cagctattgt tgatgccgat ttgttcaaac acatcaagca tatgatgtgg tcaactgtcc
    17221 cggcatttgt tgtttctttc tttttattca tgattttagg acacacaagt aaagccgcaa
    17281 gtctttcatc tattcgtgaa gtgactgatg ttctagaagc aaactttact atttcaatct
    17341 ggtcactgat tcctttattt ttaatgttac tgtgtgcatg gaaaaaggtg ccggcaattt
    17401 taacgatttt gttgaatatc gctgtagcag taggaatgat ttttattcaa aacccacaaa
    17461 cttccttaac gaagttggct ggggtcatcg aatcagggtt tgtttcaaat actggaaacc
    17521 aacaaatcga ttcactgctt agccgcggag gaatcgaaag tatgatgcct actgtgtcat
    17581 tgattattct aactttgtca ttaggtggat tgttgatcga atttggcttg atctcaaccg
    17641 tgatggaacg agtatcaaga aagatgacga acacaccaaa tttgattttt acaacattga
    17701 tgacaagtat cggagtaaat atttttattg gtgaacaatt cctatctgtt attctgcctg
    17761 gtaatgcatt taaagaaact tatgaaaaag caggatttga tccaactgta ttaggtagaa
    17821 cgctagaaga tggtggaaca gtaatcaact acttagtgcc ttggggaatt gccggaagtt
    17881 tcgtagcaag tacatttggt attcctacac tcacttactt gccattcgta tttttcagtt
    17941 tgctttctcc tgtgttttcg atggtcagtg ccttcacagg ttggggaatc tcaaaaaaat
    18001 ttgtgaaacc gcaagaagca gtaagcaaga cagcgcaatt agaacaataa aaacataaaa
    18061 agggattgtg acaagacttt tgtcacaatc ccttttacga ataaacggta ttcaaaagcc
    18121 agtttctcgg tattaagcca aatttataca aaaatgtgga aagccatttc acaaaatttt
    18181 tcttaatacg cgaaactaag cgactttttc acaacctctc gataaacggt gttcaatcag
    18241 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt tccatttgtc
    18301 tctctaatac tcaggtcaga acgctgattt tacaacctct taataaacgg tgttcaaaag
    18361 cttgcatctg cggtaataag cctaaccaaa cgaaaaatag gagaagctat ttttgtttgg
    18421 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
    18481 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
    18541 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
    18601 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
    18661 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
    18721 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
    18781 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
    18841 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
    18901 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
    18961 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
    19021 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
    19081 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
    19141 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
    19201 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
    19261 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
    19321 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
    19381 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
    19441 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
    19501 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
    19561 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
    19621 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
    19681 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
    19741 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
    19801 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
    19861 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
    19921 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
    19981 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
    20041 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
    20101 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
    20161 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
    20221 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
    20281 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
    20341 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
    20401 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
    20461 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
    20521 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
    20581 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
    20641 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
    20701 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
    20761 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
    20821 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
    20881 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
    20941 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
    21001 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
    21061 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
    21121 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
    21181 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
    21241 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
    21301 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
    21361 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
    21421 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
    21481 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
    21541 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
    21601 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
    21661 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
    21721 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
    21781 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
    21841 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
    21901 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
    21961 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
    22021 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
    22081 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
    22141 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
    22201 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
    22261 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
    22321 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
    22381 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
    22441 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
    22501 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
    22561 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
    22621 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
    22681 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
    22741 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
    22801 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
    22861 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
    22921 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
    22981 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
    23041 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
    23101 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
    23161 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
    23221 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
    23281 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
    23341 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
    23401 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
    23461 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
    23521 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
    23581 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
    23641 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
    23701 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
    23761 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
    23821 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
    23881 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
    23941 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
    24001 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
    24061 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
    24121 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
    24181 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
    24241 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
    24301 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
    24361 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
    24421 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
    24481 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
    24541 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
    24601 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
    24661 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
    24721 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
    24781 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
    24841 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
    24901 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
    24961 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
    25021 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
    25081 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
    25141 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
    25201 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
    25261 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
    25321 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
    25381 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
    25441 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
    25501 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
    25561 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
    25621 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
    25681 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
    25741 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
    25801 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
    25861 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
    25921 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
    25981 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
    26041 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
    26101 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
    26161 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
    26221 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
    26281 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
    26341 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
    26401 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
    26461 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
    26521 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
    26581 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
    26641 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
    26701 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
    26761 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
    26821 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
    26881 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
    26941 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
    27001 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
    27061 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
    27121 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
    27181 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
    27241 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
    27301 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
    27361 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
    27421 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
    27481 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
    27541 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
    27601 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
    27661 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
    27721 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
    27781 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
    27841 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
    27901 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
    27961 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
    28021 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
    28081 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
    28141 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
    28201 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
    28261 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
    28321 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
    28381 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    28441 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    28501 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    28561 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    28621 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    28681 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    28741 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    28801 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    28861 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    28921 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    28981 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    29041 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    29101 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    29161 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    29221 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    29281 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    29341 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    29401 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    29461 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    29521 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    29581 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    29641 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    29701 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    29761 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    29821 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    29881 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    29941 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    30001 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    30061 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    30121 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    30181 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    30241 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    30301 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    30361 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    30421 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    30481 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    30541 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    30601 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    30661 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    30721 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    30781 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    30841 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    30901 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    30961 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    31021 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    31081 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    31141 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    31201 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    31261 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    31321 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    31381 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    31441 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    31501 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    31561 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    31621 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    31681 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    31741 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    31801 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    31861 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    31921 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    31981 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    32041 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    32101 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    32161 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    32221 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    32281 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    32341 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    32401 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    32461 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    32521 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    32581 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    32641 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    32701 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    32761 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    32821 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    32881 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    32941 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    33001 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    33061 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    33121 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    33181 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    33241 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    33301 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    33361 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    33421 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    33481 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    33541 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    33601 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    33661 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    33721 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    33781 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    33841 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    33901 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    33961 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    34021 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    34081 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    34141 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    34201 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    34261 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    34321 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    34381 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    34441 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    34501 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    34561 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    34621 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    34681 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    34741 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    34801 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    34861 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    34921 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    34981 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    35041 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    35101 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    35161 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    35221 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    35281 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    35341 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    35401 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    35461 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    35521 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    35581 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    35641 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    35701 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    35761 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    35821 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    35881 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    35941 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    36001 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    36061 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    36121 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    36181 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    36241 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    36301 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    36361 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    36421 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    36481 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    36541 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    36601 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    36661 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    36721 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    36781 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    36841 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    36901 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    36961 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    37021 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    37081 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    37141 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    37201 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    37261 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    37321 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    37381 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    37441 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    37501 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    37561 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    37621 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    37681 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    37741 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    37801 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    37861 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    37921 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    37981 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    38041 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    38101 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    38161 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    38221 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    38281 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    38341 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    38401 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    38461 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    38521 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    38581 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    38641 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    38701 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    38761 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    38821 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    38881 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    38941 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    39001 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    39061 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    39121 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    39181 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    39241 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    39301 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    39361 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
    39421 tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttc tgattccgcc
    39481 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcagtt
    39541 tttttatgcg tgttgttgtg cgttgtagta ttcttcttcc attttttgca tacgttcacg
    39601 ttctttttgg ttataccaag gtgaaacggt aaatgcaagg acatcattca gcaagtaaac
    39661 agagctgttc acgaacatcg ccaaacttgc agatccatgc gtaaatgaaa tataccaaag
    39721 gaccatttga gcaagccctg aagctaacca ccagtaatat tggttgttgt aacgcaagaa
    39781 actgatgata ccagcagtca agctaataga aaaagcaatg gcatctaccc acggccgagg
    39841 atcatctgta taacgaccaa tcaggaatcc tgaaacaaga tagacaagga aagtactgat
    39901 gattgcgata aaccagcttt ttccagtgaa ttttcggatt tttgaagcca tattatgatt
    39961 ccattcttta cttaacaata cggggatatc caatgtgatc atataagcaa tctgttcacc
    40021 gatacttaaa tagttctttg ctgaaaaccc aacgataata aaacaaatcg cagacaaaat
    40081 acctaaaata ccgttgactg attttgccgc attgatggat aaaatacaca atacaccaag
    40141 cgtggtaccg ataaatgtaa taacggacag ccaagtgatg ggactattga caagagtcat
    40201 gacctggcag ccaagactga aaaagaaaag ataataattt tgttgcggcc agccttttag
    40261 ctgatgagct aaaaactgaa aatatgactt catgtatgag gactccttaa gacactaaat
    40321 gtagtgtgtt ctgtatttac taacacaatc tattgtatgc ttttcttgaa aaaaaactag
    40381 tctttcaatt ataaataatt tgtgttatga taattttctg tttgttttct tgaaaatata
    40441 atttcttact ttcatttatt atattgggaa caggaagagt gtcttggagg acctactttc
    40501 tgtttttttt gccttttttc ttataaaaag gaaaaacagc tatttttttc aatctgacac
    40561 atttacaatg gaactggaga ttaatataga tccgagctta aacgaagaat aggagtgctc
    40621 atatgaacct tacaacgatc catcatatag ccatcattgt ttcagattat cagaaatcac
    40681 gtgattttta tgttaataaa ttaggatttg aagtaatcag agaaaattac cgcaaagaac
    40741 gagatgacta taaattagat ctgaaactag gaagtgctga attggagatt tttggaattg
    40801 ctaatgcgcc acaacgacca aactatccag aagcttgtgg cttgcgtcat cttgctttca
    40861 aagtagacga tattgaagaa acaattgcag aattgcagtc cttagggatc gaatcagaac
    40921 cgattagaga agataccttt acaggaaaaa aaatgacgtt tttctttgat ccagacggat
    40981 tgccattaga actgcacgaa taaagataac aaaattgtca cttttttttc gtttcattcg
    41041 atttttcatt cgttcatttc ttgatattct ttaaaaaaaa gaaacaggag aatgaagaat
    41101 gaaaaaaatg atgtttggtg ttgctgccct catcgtaata gggattaccg gtatgacggg
    41161 actgctcaat gccgcttttc acatggatga tattagtgac caggaatatc ggatctcatt
    41221 gaaaaaagca gcagcaatcg taaaaaacga aacaaaagaa cataacatat ctgctattca
    41281 ttttgcagtt gaaaaagatt tggagaatcc tacgaatgca acatatgaat accagttttt
    41341 ttgtgaagga caagtcatag cagtcaatcc atccactggg aaaacaaccg tacgaaatga
    41401 cacggaaaag gccgcagaac atctttttga tatagaaaaa acggaagagt tcaaacaacc
    41461 gcaagcagcg atgaaggaag cagtcagcta ctacgggaaa aaagcaaaag tgaaagagtg
    41521 gacacttcta gtcaaagatg aacgtcctta ttataaagtc cgattaatag atggagaaca
    41581 ggaccgtcag gtatggatat ctgcatagaa gaaggatatt gcttctcttt tttacaatat
    41641 aagagtaagc taagagagta aagatgaaag gaagttgaga taacatggaa aaaatgaaat
    41701 gccctaattg cggaaaaaaa tttgcatacg aagaagtcaa taatgtagtc gaacataatg
    41761 ataaagaaat gccagtcgtc tgcccttatt gccgaactga agcggcaaga atcgttactc
    41821 atggttattt cattactgaa aagattgaag attttttaaa ataacgattt tgcgatttct
    41881 ttttctgcat gttagaatgg agaaaaggag aggaaactat gattcgtttt gcgagaaaag
    41941 aagatgctcc agaaatcgcc ccattgatct tagtgattct aaaagatatg gaactgccat
    42001 ttttgaagca gacaggggaa caaaagactt tagagatttt agaagaagca atcactgatc
    42061 caaattatcg ttacagttat tcacgaggta tcgtagaaga aatagacggt aaagttgcag
    42121 gagttgctta cggttataca gatgaagaag aatcaatcat cgatcagcca ctggtaccta
    42181 tcttagagaa atatcagcta gatgcgtcta cacgtttgtt cattgataaa gaaacgtttc
    42241 ccaacgaatg gtatttggac tctatcagtg tatcagaaga ttttagagga caaggcatcg
    42301 gttcgcgatt attagaagca ttacctaaat tggcgaagaa agctaatcgc tccgtcatcg
    42361 gtctaagtgt ggatgaaaaa aatccaaagg ctaaaaaatt atacgaacgc catgacttca
    42421 aagttgtcgg tcagcggatg atcagtggtc atctttatga tcatatgcaa aagaatatct
    42481 aaaaagatgt acctgaaatg cgtatttccg catttcaggt acatcttttt aacttaagtt
    42541 aagtcggtaa gaaacaggct tatcgcgact tttgtcttta cggtagagcc agtaatctaa
    42601 aaccgcagca atacaaatac aaactggagc atcttcatca tttgagacat ccaatacata
    42661 gtaatctcct gaaaaaagtg ttgctttatc cattttcatg atttgctgat tgaaatgatg
    42721 gatttgatat tgatgattat agatatcacc ttggactgtc cagtgaagtc tacgaatata
    42781 ataaaagtca ccgggccaat tgaatatttt ctgcatggtt cccacctttt cgaactcttt
    42841 atacagctca aaacgcgtac cgaatgtcag gcttatctgc ttgatgtgag caacgagatc
    42901 gccattcatg gcataaagag acaatgcatc gccctttgtt ccccagcgac caaccatcaa
    42961 aaacagggaa tgtccttttt cgtttttgac aattgtccga gttgcacgtc caagctgctt
    43021 ttcctgaatg aaaaattctg acatctatat ccctcttttc tagataaaac tacaagtctt
    43081 ctgcaatcgc gcgcaatatg gcttttccta cgccagattc aaggtttgtg tccgttgtat
    43141 atttggcata atcttttact tcgatttctg catttcccat tgcgaaactg actcctgcta
    43201 cttgcagcat gctgacatca ttgaagttat cgccaatcgt catgacttct tctaaagaaa
    43261 tcccacgctc gtgagcgaca tgggcaacag caattccttt ttgagcgttt ttatggttca
    43321 cttcgatatt gttttgacca gaggaagtaa cggctagatc gcctaaaaga gaaatctgct
    43381 ttccaactgg acctaataca gctggccctt ctgtatgaaa acaaataatt tttaaaactt
    43441 ccagattctt ttcttccaaa atagcacgaa tactgtccac gtatgtgatg tgcaataact
    43501 ccagcttagc tgaggccatg gcaatcgcca ttttatacgt aaggtgaggt aaatgggtag
    43561 ccaccatgga agcaaaactt tcaattcgtt tcgcttgatt ttcagaataa agaccatcgt
    43621 tggttgctac ttcatagtaa ataccgttag catctagaat atctaaaaca gagattgctt
    43681 gattatttgg aatcgacacg gtaaacaatg aatttccttc tttatcaaat acctgtgccc
    43741 cgttcaaagt gatcatcgca caatcgattc ctacctcatc taaagctgcg cgagcttctt
    43801 gatagtttcg accagtagcg accatgaact caacaccggc atcatttgca aaacgtatcg
    43861 cctcggcatt ttcaggagaa acactcatat gggcatctaa taatgtgccg tccatatctg
    43921 atgcaattaa tttgatcata atgattatcg ttcctttctg cttcttgttc ctagtttacc
    43981 atattaatca agtgctgagg gtgaagattg tcccttgtga ttgaagaaaa tgtgagttat
    44041 tctttttatt aggaggtgga gtttttgaaa gaaatcattc ataacagttg gcaagaagtt
    44101 ttgtcctcag aattttcaaa agattattat ctgcatctaa gagaattttt aaaaaaagaa
    44161 tatgcgtccc aaaagatcca tccagatatg tatcatatct atgaagcatt ggaattgact
    44221 ccttatgaag aagttaaagt ggtgatttta ggacaagatc cttatcatgg agaaaatcaa
    44281 gctcatggct tatctttttc tgtgcaacct ggcgtaaaga tcccgccatc gttaagaaat
    44341 atctataaag agctttatga tgatcttgga atcgcacctg ttcaacatgg aaatctagtg
    44401 tcatgggcta aacagggagt ccttttactc aacaccgttc ttaccgttcg ggaagggcaa
    44461 gcgtattctc atcgcgggaa aggttgggaa agattaacag acacgatcat tgaaaaatta
    44521 aatgaacggg aaaaaccaat cgtgtttatc ttgtggggaa aaccggcaca agaaaaaatc
    44581 aaaatgatcg ataagagtcg ccatatcatc ataacatctc cacatcccag tccgttatct
    44641 gcaagtcgtg gcttttttgg ttccaaaccc ttctcgaaaa cgaatgatgc gctccttgcg
    44701 ttaggagaag aaccgattga ttggcaactg cctgaaacag tgtaataaaa cagcgctttc
    44761 ctgttatagt gcagttcgct ttgtatgtga aaaacttaat ttttccagca tttttgactg
    44821 atacagataa aataaactgt tctttttttc tggaatagtg tatgataata catataaaaa
    44881 cgtctggagg tcatcttgtg gaattgtttg atagtttaag atttaaaatt gttcgccgtg
    44941 ggatcaaaat cgttttcccg gaagctactg atgctcgtat tttaggcgca gctgcccggt
    45001 tgaaagcaga agaacttgtg gaacccattt tagtagggaa cgaagaagaa ataagaaatg
    45061 cagcgcatgc cagaggaatc aaaacatcta gctttacgat catcagccct gataattacg
    45121 ataagtggga tgaaatggtg gatacttttg ttgaacgtag aaatggaaag gcatccaaag
    45181 aacaagcaga aaagatttta aaagatgtga attactttgg tacgatgctt acgtatatgg
    45241 ggattgcaga cggaatggtc tctggagcga tccattcgac tggcgacact gttcgcccag
    45301 cacttcagat catcaaaaca aagcctggca tcagtcggac aagcggagcg atgatcatgg
    45361 tacgtggaag agatcaagaa aaatatgtct tttcggattg tgcgatcaac gtgaatccaa
    45421 ctgcgcaaga attggctgaa atcgccgtag attctgcaaa aacagcagaa ttgttcgata
    45481 tcgaaccaaa agtagcaatg atgagcttct cgacaaaagg ctcagcgaaa tcgccagaag
    45541 tagacaaagt agttgaagca actaaaatcg ctaaaaaact agcgccacaa tacatgatcg
    45601 acggcgaatt gcaatttgat gcttcttatg ttccttcagt tgcacaactg aaagcgccag
    45661 attcagaagt agctggtcaa gcaacagtct ttgtatttcc agaattgcag tcaggaaata
    45721 tcggctataa aattgctcag cgtttcggta actttgaagc aatcggacca attctacaag
    45781 gcttgaacaa accggtttct gatttgtctc gtggagcaaa tgaagaagat gtatataaat
    45841 tatcgattat cacagcagca caaacattga ttgattaaca aatacacgcg aactagccaa
    45901 aaacagcagc tgctgttctt gactagttca ttttttcttt aaaaaaactt cattacgcga
    45961 tatactggca aaaagaaaga agagcgggag aagatgaaaa atgatcgaac tatcaggatt
    46021 aacaatgaca gaacaattcg cagaggcaat tggtcattcc gcaatgccag gtgacaatct
    46081 gatattgact ggtgatctag gtgcgggtaa aacaacgttg acgaaaggaa ttgcccaagg
    46141 attagggata acgcaaatga tcaaaagtcc gacatacacg attattagag aatatagtca
    46201 aggtcggatt ccgttgtatc atatggatat ttatcgggta gcagctagcg gagcagattt
    46261 agggttagat gaatattttg aaggtgatgg gctttccgtt gttgaatggg gaaatctatt
    46321 agaagaagca ctgccggagg attatttgga actgatttta gaaaaaagtg atacagattt
    46381 agaaaagcgg tatgtgaaac taatctcata cggggagcaa agtgaaacct tcaaacagcg
    46441 aattctagaa aaatgggctg aaaatcatga gtgaagaagt tgggatcatc ttgcgggaag
    46501 cagtgccagg agatgcaaaa gacattcttt tgatgatggg acaagttaat aaggagacag
    46561 aatttttagt tttggatgaa gcagaactcc tgttaccacc agaaacctta gaagaagagt
    46621 tggattatat atacgaaagc aataataatt tattgctgct tgcaatctat gaagggacga
    46681 ttattggaac agcatctgtc aaggcagaca gtcaatttcg tttgtctcac gtgggagaag
    46741 tcggtatcag tattctgcag gaatattggg gaatgggact aggcactctt atgctagaag
    46801 aaatcatcag ttgggcgaag gaaatgggtg tactttttcg attggagctt gacgttcaag
    46861 ttcgtaatga aagagccgta cacctttacc gaaaaatggg cttccaaatc gaagcggtca
    46921 tgcctagagg ggcaagaaca gatctaggtg aatttttaga tgtatataaa atgagttatt
    46981 tgattgaata gaagaaatag gaaaagagtg agaaactgtc atccttttga gaaggcagaa
    47041 aaacacacga agggactgtg acaaaagtcg tgtcgcagtc catttttttt gaataaacgg
    47101 tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    47161 tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttggtaaacg
    47221 gtgtccaaaa gcttgcatct gcggtaataa gtccaaccaa atgaaaaata tgagaagcta
    47281 tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccaata
    47341 aacggtgttc tcctgccagc tcctcggtca ccagtcacaa tatttaataa acatgagaag
    47401 ctattttttg aaattgctcc ttgtgactcg gagtgaaacg gcaggctcac gacctctttg
    47461 tgtgtttcat aagtattctt ttactttata gtcacaagtt ttttcaatgg atctgtcccg
    47521 aatttttttt cttgataaag caggatttcc gcacatgcac gactatcttc caatgcatca
    47581 tggtgatgat ctaattggat gttcaaattt tcgcacacag tgttcaatcg atgatttgga
    47641 aattcaggaa acaggcgacg gctacttttc actgtacata aagaaagata attaggctgt
    47701 tcaagatgat aataatctaa gcatcccgct aagacctttg tatcaaaagc agcattatgt
    47761 gcaacgacta aacgattttt ttgaaaatat ggttggatag tttgccagac ttctgggaat
    47821 ttcggcgcat tgcgtacgtc ttcttgatgg attccgtgaa tttggacatt tcgccagaaa
    47881 aaaggagtct caggctgtat caatgtatag aattcatcaa cgatctggct attttttacc
    47941 atgactagcg caagagaaca agcactataa ggctgatgat tggcagtttc aaaatccatg
    48001 gcaacaaaat tcatttctgt aaaacctcct tgggaaatta ctgtaacata cttttgaaaa
    48061 aagcaaataa atttaacaaa aaacatagaa ttttttatgt aatatttgtt gagaaaagct
    48121 agtttttgaa ctcattcctt gtgatttgga gttagatggg gatcatgact tctcggtatt
    48181 aagtcaaatt tatgcaaagt tgtgaggaac cattttcaaa aaaatttctt acgactcgaa
    48241 agtaggcgac tttttcacaa cttctccact aaacggtgtt caatcagctg accctcggta
    48301 ttagctcaaa aaatagcaaa atatgaggaa ctattttgcc atttgtttct ctaatactca
    48361 ggtcaggacg ctgatttcac aacttctcaa taaacggtgt tcaaaagctt gcatctgcgg
    48421 taataagtcc aaccaaacga aaaatatgag gaactatttt tgtttggctg ttcttattac
    48481 ttgatgcagt gcagcttttt cacaacctct taataaacgg tgttcaaaag cttgcatctg
    48541 cggtaataag cccaaccaaa cgaaaaatat gaggaactat ttttgtttgg ctgttcttat
    48601 tacttgatgc agtgcagctt tttcacaacc tcttaataaa cggtgttcaa aagcttgtat
    48661 ctgcggtaat aagcccaacc aaacgaaaaa tatgaggaac tatttttgtt tggctgttct
    48721 tattacttga tgcaggacag ctttttcaca atctctcgat aaacagtgtt caaaagccag
    48781 tttctcggta ttatgccaaa tttatgcaaa atgtggaaag ccattttcac aaaatttttc
    48841 ttaatactcg aaactaaacg actttttcac aacctcttta tttcaaatct aattctactg
    48901 gacaatgatc actgcccata atgtccgtat ggatctttgc atcagctagt cctggctcta
    48961 aatctttgct cgtcaaaaag taatcgatcc gccaaccagc attgtttttc cttgaattga
    49021 atcggtagtt ccaccaagag tagacacctt cctgggttgg ataaaaataa cggaatgtat
    49081 cgataaaccc gttatccaac agtcgagaaa gagccgctct ttcttctgga gtgaatccag
    49141 catttttttg attcgttttc cagtttttca aatcaatctt ttgatgagcg acatttagat
    49201 ccccacacac gatgactggt ttttgttttt ttagattttc caaataatta taaaaggctt
    49261 cttcccattc taatcggtaa tctaaacgtt ttaattcgct ttgagaattt ggcgtgtaac
    49321 aggttacaag aaagaaatcg gaatattcca aagtaatcag acggccttct gtatcatgga
    49381 tatcgatacc cattccgtaa gtggcattca atgctggttt tttagcaaaa atcgctgtgc
    49441 cggaatagcc ttttcgttcc gcgtaattcc aatattgata ataacctgga agatctaaat
    49501 caatttgtcc tgcttggagt ttcgtttcct gcagacagaa aaaatcggcg tctaactcat
    49561 gaaaaacttc taaaaaattt ttatttacga tggcacgaag cccgtttacg ttccaagaga
    49621 taaatttcaa taaaagcaca ctccttcgaa ttaatttcct tacctttgcg atactattgt
    49681 acctcatttt agtaaaaaat ggtgtaagat gaaagtaact aaatgatcca ggaggaaagt
    49741 agatgaagat tggtgtaatc ggtgtaggga atatcgcaga aaaagcatat ttaccaactt
    49801 atgcgaaaaa acaaggaaca cttgattttt attttgcaac aagaaataaa gaaacaaaag
    49861 aaagaattca aaaagagtac gggttccaac atctatatga aacgatcgat gagcttttag
    49921 aagaaaaaat cgaagcatgt atgatccacg cagcaacaaa agtacattat gaattagcca
    49981 aaaaatgtct agaaaatggt atccatgttt atattgacaa accactgagt gtccatctaa
    50041 atgaagttcg tgaattgcaa gaattagctg ataagaacca aataattttg atgatcggtt
    50101 tcaaccggcg ctttgctcct atggtagaag aattgaaacg tatcccagat aagcggttga
    50161 tccacctgca aaaaaatcga atagcggcaa aagaaacgac agagttcgtc ctttatgatt
    50221 tgtttttaca tcttattgat acagctgtgt atttattaga tgaacccatt ttgagaacaa
    50281 aataccagat aagagaaaca aaggaatttt tggaatatgc cattttacaa ttagaaacac
    50341 aaaatcaaac ggctatttta acgatggatt tagcaagtgg cgctaatata gaaaaatatc
    50401 aagtaacaag taaaaatggt acttatgaag taaacgatct gacccagctt gtcattcaag
    50461 aggctgatgg aaaaaaagtg aaagaatttg gcgactggac aaacacgcta gtcaaacgcg
    50521 ggtttgagcc aatggtagag gccttctttt ctcagatccg tttaggtaaa tcggaaacag
    50581 aattgcttaa acaaaaagaa gttgtaagaa gtcatgaatt atgtgaggaa gtgcttaaag
    50641 atcactaccg tcatcaactt tgaacaagac tggcaagatt gtattaagcg tgatagaata
    50701 atgaggacgt ttgagaaaag aaaggaaaat cctcagtgaa taaagaagaa atagttgaaa
    50761 gcttagccac gattactata ttgtttgatg aaccattgat gaactatacg tttacgaaaa
    50821 caggtggacc agctgatgtc cttgcattcc cgaaaaaaca agaagaagta aagcaaatca
    50881 tcgattattg ccgaatccat gatatacctt ggatggtatt aggaaatgca agcaatctga
    50941 tcgttcaaga tggcgggatt cgcggtgtag tcatcatgct gactgaaatg aaacagattc
    51001 atgtcaaagg aacgatggtc attgcggaag caggtgcctc actgattgat acgacttatg
    51061 cagctttggc agaatcattg actggatttg aatttgcttg cggcatcccg ggaagtgtcg
    51121 gtggagcggt ttatatgaat gcaggtgcgt atggcggaga gatcaaagat gtttttgctg
    51181 aagtggatct tttgcttgaa gatggaacat tgaaaacttt gacaaaagac gaaatggtat
    51241 tttcttatcg tcacagtaaa gtccaagaat tacgtgctat cgtattggaa gctcgattca
    51301 gtttgcaaac aggggattat gaagcaatca aagcaagaat ggatgagttg accgaattgc
    51361 gtcaatcaaa acagccatta gaataccctt catgcggaag tgtctttaag cgcccagtag
    51421 gacactatac aggtcagctg atccaacaag caggattgca aggcctgaaa tggggcggtg
    51481 cacaagtctc tgaaaaacat gctggattca tcgtcaatat cgatcatgcg acagcgacag
    51541 attatattga attgattgcg cacatccaag aagtaatcaa agaaaaattt gatgtgtctt
    51601 tagaaacaga agtgcggatc attggagaaa aacaagcaat atcagaaaca agcagaagtt
    51661 tagccaatta ataagtaatg gaacttaaaa taagtaaaag agcaggacgg tggccgtcct
    51721 gctctttttt gtggttgttt acgcaaatgg cgcaaatctt gtacatttta acacaataaa
    51781 acaaaaaata tagaatattt cctcataaat ttttccattt tttgcaatat tctcaaaaaa
    51841 aagataaaaa atgagaataa ctgatgaaaa aacaagcata atccatcata aaatcagtat
    51901 aggtgaaaag atgaactttc atctgcatat caagtaattg gtatgaggca ttttttgagg
    51961 tgtaggatat gaagttggat taccttatag aagaggaaga tagagaaaaa ctgctgctag
    52021 ctagatattt agaatttgag aaaaagaatt atgtagtgct gcctaagtta tatgaaacat
    52081 taggtttgtc taaatttaaa acaagaaatt atatcataga attaaataaa gaatttactg
    52141 aattcaaaac aaagccagag atccaaattt cagaagaagc agagatccat gtcgaacgtc
    52201 tgaatttgac ggatatcaaa aatttaagaa gaatctactt tcaacgatca aaaattgctc
    52261 gactgttaac ggagatcgtg gattcaaaag taacgatcga aaaatttgca gagaatcatt
    52321 ttttaagcat atcaagagcc tatgtaaaaa gaagagaatt agtctctttt cttgaacagc
    52381 atcaaataaa gttaaagaaa aacatattag ttggggaaga aataaacata cggaacgtat
    52441 tatttggggt atatttcggg gtctttaatg gtttttctat gccatttaga gaaagcgtca
    52501 tgaatgaagc aaagagtatc gtgcaatatt ttgaatatct gttcaattta aaactgtctg
    52561 caacaaaacg aatcaaattc aatttgctaa ccactattag tttgttgagg gcctatggga
    52621 atcaaccaat cggaaaacgt ttcttttcag atgaatactt taagagctca gaaggaatga
    52681 agatcatcaa ggatttatca catatttctt ttcttcatat ccctaaagag cagatcattg
    52741 atgagatttc ttatatcatg ttgtttttga tcacggaaga agaatttgaa ttagatggcg
    52801 catttggact tgcggaagct cattttcatg atatccacat gattagtcaa aaaatgtcag
    52861 tggaattgat tgatcaatta ccattttcga ttgaaatctc tgaagaagaa agactatata
    52921 tacgaaaaga acttcgtatg ggtatgatgc atgtcaatcg aaaaaattgg atattcgact
    52981 ttgagatgac tagctttacg tctaaaaagc agctacagtt tttcttcgag acgtatccaa
    53041 tattcagtac agcaatccaa aaggtcatac aagagaataa acgctatttc ttgggaaaag
    53101 atgaacgttt catgattcgc ttattttatg attatctctt tttattggtc ggtatttgtc
    53161 cggtatcata ttttgaagca ccgattcatg tctgtattga tttttcgcaa ggatctgctt
    53221 ataccgatta cattatttct caagtgaaag gatttaaaaa ttacaatatt gtgattgaga
    53281 gtcgaatcac ttctcgtacg aatatttatt tatctgattg tttacttgaa aaatttaata
    53341 aagaacagat aatttggaaa aatccaccga ctccagatga ctgggaatat tttggaaata
    53401 ccatcatcag agaaaaaaat agacatttaa ctgaaaaaat cagctagtat ttatggatgg
    53461 cagctttgtc tcacatattt attagctgat ttatgttttt ttcaaagaaa aggtaactag
    53521 atataactag atataactag atatattact taacaaaatt aaaaagtaca taagttatta
    53581 ttccgatttt cttgaaacaa taaaaaatca aaaaaaatac tctcctaata gtccatcttc
    53641 ttttgatttt atttatataa tgaaatcatc ttatgaaaat tactacttca tgtttagaca
    53701 agagtaggga ggactaagaa taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac
    53761 acgcttgtcg tttagataag gaacggtcgt gttctgaaaa acactacgtt aagtttatga
    53821 tgcgcacgat gctgatcagt ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa
    53881 cgacacaaac catcaaggtc gtacgtacct ctgtttctga gaccactgat tcccctatca
    53941 ctattactca tactttcact gatgcaaatg gaaaacagct acttcctgca ttgacttcct
    54001 ataataaagt cagttatcct ccaacgataa accactactg gttaattcct aacaaaacat
    54061 tatggacatc ggaaaccgga agcagtgtgc cgctttctat cttttccgat atgactgatc
    54121 ctacaaaagg tgatcagtta aaacagatca ctgatatttt gaatcatgct ttgacagata
    54181 taaaggacgg tggaagcgca caagtttctt atgtatatga aaaagattat tcacagtttg
    54241 aaagaaagaa aatcgatgtt ctggcaggga caaaagtttc gatatctgat ctggtagaaa
    54301 ggctgaagaa cgcagatgga acacaaggag atatgtccaa agtaatttca aacaatggga
    54361 atatcgtgga tacgactaat acaggaacat ctactatttc cttgtcctat tttgatccaa
    54421 ttgctttaaa aacaatgact gcatctgctg tgatccatgt tcgtgctgat caaacagcta
    54481 tcaaaggaaa agatcttcac ttaagaacag gagagagtta cgcccctaaa gatcttgttg
    54541 aatatgtaac aaaaagcgat ggaacttcag gaaataaaga agaaatcaaa atgaacggga
    54601 cagtagatac tacaactccg ggagaatatc ccgttgtatt aacttatata gactcgaaaa
    54661 catcaaatgt ggcagaaaca acagctgtag tttatgtaga agcatctgct gcttcatcga
    54721 ctgtcatcac tcattttata gatagtcaaa caaataaaga acttcaagca gaaacacata
    54781 tgagtagtgt aaatcgtatt cctgatttgc gcctacctgc tgaattttct gatcgagtgc
    54841 caaatataga tttatcgact tatgaacaaa atgggaaagt cacttctatt tctgattgga
    54901 tgcaagcaaa cggattagac agaaaaagtc actggtcgga gatccttgaa gtacttcact
    54961 cagaactgat gaataatagg aagcaatcag gaggggaaat tacctttact tatgtttata
    55021 taccagacaa tcgttcatta gaaggtcatg atcttattgt tcatccaaac gaaaaaataa
    55081 acaaagaaga gttgatccaa tcagcaacaa attccttagg taaacccgta ccgttaaagg
    55141 atgtatcggt tacaatcaat gaagtaccat tggaggatgg ttatttgacc aaacaaacag
    55201 gtgattttac gattatgtat agttatcatg acccgtattc actccaagaa ctccatgctc
    55261 agtcactatt acatgtttta ccattaaaca aacagataga tcaaaaagaa caaaaaagac
    55321 gagaagaaag cttgtcagaa aaaaaacaac aaatggctca tgcagtttct ccaacaggga
    55381 aagtaaaaca aacaagaagc ctagagttag caagaatatc tagaaaaatc tcagaatcta
    55441 ataaaacgat aaggcaagaa gctcatttgg ccccacaagt ttctggattg gaaaatgaaa
    55501 ttttagagcc taaaataaaa caacaatcag aaaaagaatt tgttgctaaa cagtcggaag
    55561 atcagaagga atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca actattgatt
    55621 ccaccgatga gcatctgcag ccgcaagtac cacttcctag tccgggaaca cccgcacctg
    55681 gtggaggtat tccttcttct gctttaggag atttcatgcg aggtatggca ggaacatggg
    55741 tatacgctaa tcgaaatgaa tttgacgcag atgactggtt ctgatttata tctctatctg
    55801 ttatagtcaa ataagtagct gagtcatatg ttatttggca taatggagac gaggagggat
    55861 agaaaaatgt tagataaaac gttaccttac gcagaaatct ggatgacaag accacttcaa
    55921 caaaatgttc caatcacgcc tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa
    55981 gatgaatctg cttgggcgga gattgagact tccgttgggg aatttcaaac gacagatgaa
    56041 gcacttgagt attttcaaca aaactttgct ccttatcctg aagaacttga aagaaggatg
    56101 ttttttgtag agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa
    56161 tcaagagagc aagtgtatcc ggtttttcat tggttagcag tgaaaccaga ttaccaagga
    56221 aaagggctcg caaaagcatt gactgccaga gtcttgcaag aatttcctac cctccatacg
    56281 gacggaccaa tctatcttca cacacagaca tggagccatc aggcgattgc tctttataaa
    56341 aaaatgggtt ttacttttat tgctgaaaat ctagacggca cacctaatcc agattatgaa
    56401 aaagttatgc aggttttaag taaattagat aaataacgaa atggtgaaat aagtcctcag
    56461 tattgggata aaaaacgaaa tagtcattcc tcatatctta cttttttgag ttaatactat
    56521 cggataacta atggcatgcc atacattcaa aaaagaaggg gcagtgacaa aagtattgtc
    56581 actgcccctt ctcttgtttt ttagcatgtt ttatcgtttg atttttttag gaataaaatt
    56641 cttgatgaac gtaaattttt ggtaatcgtt tcgactatag aggtaaccaa atacactgaa
    56701 aacaactgct ccaataaaat taacaaataa atccttcata gtatccatga ttcccacgtc
    56761 taagtaacca ttttggatga ttgttttatc tgttttacca tctgcatttt gactttcaat
    56821 gattgtccga gtgatatctg gaacatgata gacagtgttt ccgtctttac ttaactccac
    56881 actattgatt tgctggatga tcgtatcttt ttgcatatcg aattgcagta atttatctgc
    56941 tgaaaattcc acaaactccc acataacacc aactgtcatc gaaaaacaga aggaaacaac
    57001 tgctagtaat aaaggagtca agttcatggt ttttgtattt ttattgagca aataaacaag
    57061 ggaaaagccg acacccccag ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat
    57121 tgaaaaatag ccataaaaat cacttaactc tcctaaaact gttgaactaa aaataaaaga
    57181 taacaaaatg atttctagca tatttggaat ctgtatcttt aatatctttt ccagaaaata
    57241 agggatttca aataaaatta atgtcagtaa taaaagcatt gtatttcccc agtgttggtt
    57301 ccagacctga tccactagca taataacgac gatgatacgt aataaaatat agattccgtt
    57361 tgtgattcta cctgcagttt ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa
    57421 ataactcctt catgtatatc cttaattata ctaagaagcg tgtgtttacc aaagctatat
    57481 gacttaaaga aaaacagagt tgatattgtg aaaaaaatca cttaatatag tataaggaga
    57541 aaagggggat acaaatgaaa aaattagttg ctggtttatt aacagccgtt gcacttcttg
    57601 ctatgagcgc atgcggcgca gatcagaaaa atacagcgca gacaagttct agtacgaagg
    57661 aatctgccga agtatcatca ggcgcttctg aacaagtata tacagatcca aaagaaatga
    57721 aagaatctta tgatgtcgtg attattggag ctggtggtgc agggatggca gcagctatcg
    57781 aagcaaaaga tgctgggttg aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa
    57841 acacatctaa atcatcaagc ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag
    57901 gaatcaaaga ctcgaatgat gccttctatg aagaaacgct aaaaggtggt ggaggaacga
    57961 atgataaaga aatgcttcga tactttgtcg atcactctgc tgatgcggtc gattggctag
    58021 atcaaaacgg tatcaaactg gacaacttaa cgatcactgg agggatgagt gaaaagagga
    58081 cacatcgtcc gtctgatggc tcggctattg gtggatactt agtggagggg ttattgaata
    58141 acgtccatga gcgggaaatc cctatttttg tcaacgcaga tgtgacagac attaagaaaa
    58201 atggggaaac ggtcaatgaa gtgactgtac atgttcaagg agaagaacca aaagaggtca
    58261 ccggaaaagc aatcgtagtt accacaggcg gttttggtgc cagcaaagaa tttattgaaa
    58321 aatatcgtcc cgatttagct gattatatta cgacaaatca agaaggatca actggagatg
    58381 gtatcaccat gattgaaaaa ttaggtggac aaaccgttga tatggataaa atacaaattc
    58441 atccaactgt gcagcaggat gagggggtct tgattggtga ggttgttcgc ggagaaggag
    58501 caatcttggt cgatcaagag gggaaaagat ttgtcaatga aatggataca agggataaag
    58561 tatctgcagc aattacagca ttgcctgaaa aatcagctta tttgattttt gaccaaggcg
    58621 tgcgtgatcg tgccacagct attgaatttt atgaccaaaa aggatatgtc acaactggaa
    58681 agacaatgga agaattagca gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag
    58741 ttgatggatg gaatacggat gttgcggaga aaaaagatgc tcaattcaac cgtgccacag
    58801 ccatggaaca tgacttatcg tctccgaatt attatgcgat caaaatcgca ccaggaatcc
    58861 attacacgat gggcggtgtg aaaatcaata cgaacactga agtattagat aaagaaaacc
    58921 agccaattag ggggttatat gctgctggtg aagtcacagg tggcttacat ggaaacaacc
    58981 ggatcggtgg aaattcagtt gcggagatca ttatttttgg gcgacaagcg ggcatacaag
    59041 cagctcgatt tgcttcggaa aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa
    59101 tcagcgtttt gagctaatac cgagggtcag ctgattgaac actgtttagt gaagaggttg
    59161 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt
    59221 tttaacttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt tattcggaaa
    59281 aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    59341 cggaagcaga agaaattcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
    59401 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttga
    59461 tcagcatgat tttattcagt tatcttcaaa atgatttttc cgatgttttt gtttgcttcc
    59521 atatattcat gcgcttgttt gactgcgctg aattggaatg tacggtcaat gattggccga
    59581 atcttgccat tttctagaaa aggcccagct ttttgcataa aagtttgtgc gagctctgct
    59641 ttatactgat cacttctagg tgtcaataat gtaccaagca gttggacacg tttcccaatc
    59701 aatacaccaa ggtctatctc aggcacgatg gtcccaccta gcataccaat caagacccaa
    59761 cgtccatcaa cggctataga agctaaattc ttttcccagt aagaagcgcc gatgaaatcc
    59821 aaaataacat cgactccccg accttcagtg atttttcgta cctcttcttg aaagtcttgt
    59881 tttttatagt tgatcaattc atctgcacca agttctttac aaaaggcgag tttttcttct
    59941 gatcctgcag taacgatgat tttcgcgtga gaaagctgtc tagctaattg gatcgctgct
    60001 gttcccacac cacttgctcc tgcatggatc aagacagttt cgccttcttg taattttcca
    60061 agccagtaaa gtgtttgata agcagtcaaa aaaacttctg agattccagc agcagaaaca
    60121 tagtctagcg tatcagggag aaggattgca cgatcggcag gcatcacagc atattcggca
    60181 taaccaccta gattgactaa tccgtatact tttgtcccag gagtaaaagt ggagatatca
    60241 cttttattct caactacctc tccagctact tcgactccca aaatagggta gggaggtttc
    60301 aaagagactg attctctttt cataatatct gttcgattga cagctgctgt atggactttg
    60361 atcagtaatt cgccttgttt tggtttagga acaggcactt caatttgttc taatacatct
    60421 acagaaccag gatgcttgat tgaaatggca cgcatggatc tattcctcct ttgcccattc
    60481 ttggaatgct gtattcaact ctttgctacc ttcagaaagt actagagtcg tacgagaaat
    60541 atccgtttct tccactagag cggcaagtac agcagcaact gtagagcgag tgatcgtttt
    60601 tgatgccttc tcattaggct tagttaattg aatggaagta acttcttcat cgttagtcaa
    60661 tgtgactggt tgaagaatga caaaatctaa gttagttcgt ttcagccatt catctgcata
    60721 atgtttagta atataataat ctgtcatgga ctcaggccat tttgctggtt catctgcata
    60781 aacagcactg accatcagat agcgagagac atgcgcattt tcagctgcga tcactgtttt
    60841 gattgcgcca tccaagtcta cttgcagaag attctttcct tgagagccgg cagcaaaaat
    60901 caatacatcg atgcctttga atgcttcggt cattttttcc acggaccaag ttaaatcaaa
    60961 agagacatac gaaacattct ctttttttac taatgtttgg gaagcaacat ctctcacccc
    61021 tgcaaaaatt tcatgggaac ttggagctaa atcttttatc aggtgtcggc caatttgtcc
    61081 gtttgcacct acaacaaaaa ttttcatttg gatcatcctt tcgacgtcaa atctttccat
    61141 tcaatagtaa caccaaaaga gaaaaaaatc agagatcggc tacgatgact atcagttagt
    61201 aagagaaggg tgcttgaagt atcttaacaa gacattgcat actgctgcag tcgcaagacc
    61261 tgcgacgatt ccttcaggta taccacttgt aaaaataacc gctagaatag ctgaataaac
    61321 agcatttaaa tttgttccga tgactttggc ataatcttgc tggaataaaa agtagatcat
    61381 attcatcact aaaatcgtat tggtcaatga accggaaagt ccagctaaga ataaagaaac
    61441 ggaatctgct ttttgtttca ttagtttgcg cagaccttta taaacgaaat aaggaaccac
    61501 cccaatcaag atgcgaggaa taaacgcgat cagcaaggct ttccagcttc catgttctgt
    61561 tccgatgact gggataaacg gagaaaagac aaaagacaaa ggtgtttgga taactgtgct
    61621 acggatcatg ctgatcagtc caaatgtacc gccaagaaaa gcacctagtc ttggtcctaa
    61681 aacgatcgaa gcaatgatta caggaatatg catggttgtt gcattgattg gaccgatagg
    61741 gataaaacct aaaggcgtaa cagctaataa aatcaaaata gctaaaaaca tcgcagttaa
    61801 tgtaaagttt tttgtatttt tcataagtac tccttttttc taagcatttc tttcattaag
    61861 agtcagcatg acctgttcga tgatcgtctg aatatcagct aaggcacctt ttccaaaatc
    61921 tccacaagct aaaagagatt ctcttggttc aatttcctga tagccgacag atctcaatgt
    61981 ttccagatta cgctgagtga tcgggttttg atacatattc gtgttcatgg caggggcaat
    62041 caattttggt gttcccactg gaagcgccat tgcgactgtt gaaagaagat cgtctgcaat
    62101 gccgtttgct agcttgccga taatgtttgc tgtagcaggc gcgatgagaa acaagtcagc
    62161 ttgttttgct agttcgatat gattgattac ctgcggattt gattcttgca tgacgtctgt
    62221 atggactgcg cgcttcgaca gagattgcaa ggttaatggc gtgataaaat gtgtgctgct
    62281 tttagacata atcacatcta cctgggcacc taatttgacg aattgacttg taagatcagc
    62341 gcttttgtaa gcagaaatac ttccagtaac gcctagtaag atctttttca ttttgattcc
    62401 tccagtctaa ttttctcagt aatcaatgca gcaatctcag atttactttg agcttcttcc
    62461 actgttccat ctttagataa gagatagccg tggtgtgtct cgccatggat ttccgtcaaa
    62521 tcatttgcga aaataaaatc agttttgtta tttatcagac tattttttgc gactgccaga
    62581 agagattcct ttggtacatc tactaatagt ttgaaaccaa tgatttttgc ttttggctgc
    62641 cattcacgta gataagaaat gatttttggt gttttcttca aaacgagcac aagatgctcg
    62701 gtgttagaag agatctttga aacagtggta tctgtctgtt cattttccgc aaaccaattg
    62761 tccagttctt cttgcgttga agaagtgggt aggttttttg ccagctgttc ttctgaaaaa
    62821 ctaaaagcag gagtaaaatc actgactgcc atactgtgga taatcgcatc gtagtgttct
    62881 tttttcgtta gttcttctaa ttgtaaaaat aagtccagtg cagattcgat cgtataaatc
    62941 gttatattgg agtgtctttt cggtttcagt gcttgtttcg tagtcacgta atcgattgtg
    63001 gtatccggat tggcagctaa ataatcagct aatgcttgcc ctaatcgtcc tgtcgaatga
    63061 tttgtaatag cacgtacttg atcgattttt tcagtcgttc ctccagctgt gattagaatc
    63121 ttcatttatg gtcacctaca tttactagtg ttcttccttg gtgtgttcca gcaagtagtt
    63181 tagatgctgt ttcaggcaat tgatccagag taatctcttg gacgaccagt tcatctgcaa
    63241 tctgcaaatc cgccatctgt tgccataaag aaagacgttg atctattggt acattgacag
    63301 aatcaattcc aatcaactgg atgtttcgca gaataaaagg caagacagtc gcgtttagtt
    63361 tgattccccc agcattgcca cataaagcta ctgcaccgtt ataagagatc aatggaagaa
    63421 gactgcttag ctgctcgcca cctactgtgt cgatgacata gtcgatttgc tgttttccta
    63481 aagcttttgt tgtctctggc agaaattcat caggtcgtac gacttgcaaa gcacctagac
    63541 tttttagcca tgtgacagac tcttcttttc tggaaaaggc aatgattgac tggaatccta
    63601 gtttatgtaa cagggctatc gctgtactgc ctacaccgcc agaagcacca gtaacgacaa
    63661 cttttttatc ggaggtcata ccttgacgga ttaaagcgtt cacacacaac atagcagtaa
    63721 accctgctgt accaaggatc atcgcttgac gcagcgtcat attttttggt agaggaacta
    63781 accagtctcc tggtactttt tgatattggc tataaccgcc tggatgggtg acgccaagcc
    63841 caaaaccagt agcaagtaca agatcttctt ttgaaaaacg tggattgtta ctttggacga
    63901 cagttccgga taaatcaatc cctggagtca tcggatatgt tcgtatcacc tgaccagact
    63961 cgctcattgc caatgcatct ttataattga catcagaata ggcaattttt accaagactt
    64021 cattttcagc caactcagta aatggcagat cactgattgc tgtatggaaa acaggttcct
    64081 ttattacctg aaaagatcga aaagcgttca ttcgtgagac actcctttta aaattttctt
    64141 ttatcatacc ataaagccta ctcttttatt cttgttcttc cgtaaattta gacaaaaatt
    64201 aggtgaaaaa taaagaatca aacaactaga atttattcca aaagataaag aaatattcag
    64261 caaaaaaaat tttacaaagg gttgcctttt tcaataaatt tcgtatacta atacgagtgg
    64321 tagtaaacag gaagtttact attagaaaac ttcgattcgg aggaaattat ggaaattggt
    64381 gagaagttaa gaaatttgcg catccaaaag aatttgacac aagaagaact aggcgaacgc
    64441 acagatttgt caaaagggta tatttcccag ttggaacgag atttaagttc accatcaatg
    64501 gaaacattct tttctattct tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa
    64561 gaagcagcga atctgcaagt ggtttattca aaagaagatc atacggttta tttcgatgaa
    64621 gaaaacgggt atgaattgga atggctcgtg gctgattcga atgaaaaaga aatggagcct
    64681 gtcttgctga cttttgaagg tgcaggagaa tataagacgt ttgaaccttc attatctgaa
    64741 acgtttattt ttgtattaga aggggaatta gagttaagct taggagagac ggtctatgtg
    64801 gcaaaagcag gacagtcgat ctattatcaa gcgacaaaac aacatcaatt aaggaatcat
    64861 ctcaaaaaac gaacaaaagt attgattgtt gcaacgcaat catatttgta atataggggg
    64921 taaaaacgtg gggaaaccaa tcatttcatt tgatcaagta gtgaaacgct atgatgatga
    64981 aacagtatta aaaaaagtaa gttttgagat cgaacaagga aaattttata cactgcttgg
    65041 tccttccggc tgtggtaaaa caacgatcct acgtattata gctggattca ccgaagctac
    65101 agaaggggac atctattttg aagggaaacg gttgaatgat ttgccagcta ataagcgaca
    65161 agtaaataca gtcttccaag attatgcact ttttcctcat atgaatgttt ttgataacgt
    65221 tgcttttgga ttgaagatca agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga
    65281 tgctttgcgt atggtccagc ttcctggata cgaaaaacgg gagattagtg aaatgtctgg
    65341 tggtcaaaga cagcgtgtag cgattgcacg agcaattgta aatgaaccga aagtattgct
    65401 attagatgaa ccgttatcgg cgcttgattt gaaactacgt acagatatgc agtatgaact
    65461 gcgggatctg caacaacgat taggaatcac gtttattttt gttactcatg accaagaaga
    65521 agcgttggca atgagtgatg agatttttgt catgaataaa gggaaaatcg tccaaagtgg
    65581 tacacccgta gatatttatg atgagccaat caatcatttc gttgctgatt ttgttgggga
    65641 gagcaatatc gtggacggtg taatgatcga agataatcta gttgagtttg ttggtaagca
    65701 atttgaatgt gtcgatggcg ggatgcgtct aaatgaacca gttgaagttg ttttacgccc
    65761 agaggatttg acgatcacga caccagataa aggaaaacta gtcgtaacgg tcgatactca
    65821 gctgtttcgt ggtgtccatt atgaaatcat ctgttatgat gaacagcaaa atgaatggat
    65881 ggtccattca acgaaaaaag ctaaagaagg aagcaaagta ggattggcct ttgagccaga
    65941 agatatccat gtcatgcgtt tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag
    66001 ctacgaagaa taggggggac aagaatgaaa acaatgcgca ggatctattc cgtcccttat
    66061 attttgtggc tcggtttatt cgtgatcgcg cctgtattaa tgatcattta tcaatctttt
    66121 tttgatatga atgggcaatt cacgctgaat aattatctat cttattttac gtcggggaca
    66181 tcgctttcta tgacgatcaa ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg
    66241 atcatcagtt acccgactgc ttatttttta acaaaactga agcacaagca attatggctg
    66301 atgctgatta ttttgcctac atgggtgaat ctgctgctta aagcttacgc atttattggg
    66361 attttcagtg tgcacggcag cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa
    66421 cagatattat tcacagactt cagttttctg ttcgtggcaa cgtatatcga gattccattt
    66481 atgattatgc caattttcaa tgcattggaa gagatgaatc cttctttgat cagtgctagt
    66541 agagatttag gcgcgaatag ctttgagacc ttccgacgag tgatttttcc gttatcatta
    66601 aatggtgtga aaagtggtgt ccaagcagta tttattcctt ctctttcttt attcatgctg
    66661 actcgtctga ttggcggaaa tcgggtgatc actttaggaa cggctatcga acagcatttc
    66721 ttggttacac aaaactgggg gatgggttct acaatcggtg tgattttaat cattgctatg
    66781 ttcatcgtga tgctcctgac aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa
    66841 tttaaatggt cgtatcttta tttagggatc gtctttttat tcttatatgc accgatcttc
    66901 tatctgattt tttattcctt caatgaagga ggaacgatga acagctttac aggatttact
    66961 tgggaaaatt atgaagctgt ttttgaagat acacgtttga tcattatcgt attgaatacg
    67021 ttcatgctgg ctttcttatc tgcattgatc gccacgatca tcgggacatt tggggctatg
    67081 ggaatctatt acacgaaaaa acgacgtgca cggacagcgt tgttgagcat gaataacatc
    67141 ttattagtct ctcctgatgt tattatcggt gctagtttct tgattttctt tacactacta
    67201 ggattcggat taggttttca gagtgtattg ctttcccata ttgctttcag cattccaatc
    67261 gtcgtcttga tggtcttgcc aaaattgcag gaaatgaacg attcaatggt cgatgcggct
    67321 agagaccttg gtgctaataa tttccaagta gtcaaaaata tcattttgcc ttatctttct
    67381 ccaggaatca tagctggata ttttatggca tttacctatt ctcttgatga ttttgcggtt
    67441 acatttttcg tcacaggaaa tggctttacg acgctctcag tagagatcta ttctcgtgca
    67501 agacaaggaa tcagtttaga aatcaatgcg ctaagtgcgt tagttttctt gttctccatg
    67561 atattggtca ttggatacta ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa
    67621 aacagaagag tacgcgaggg ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt
    67681 taggtattct catcattatt gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa
    67741 gtggcatgag tggagcaaag gtacttacca tctataactg gggagattat atcgatccta
    67801 gtttgatcac gaaatttgaa aaagaatatg gatacaaagt gaattatgaa acatttgatt
    67861 ctaatgaagc gatgttcaca aaaatccagc aaggtgggac aaactacgac atcgctatac
    67921 caagtgaata tatgatccaa aaaatgatca aagaaaaact agtacttcca ttagatcatt
    67981 cgaagatcaa aggattaaat aatattgatc cacgattttt agacttggat tttgatccag
    68041 ataatcagta ttcgattcca tatttttggg gaactttagg gatcgtatat aacgataaat
    68101 tctttgatgc aaatcaaatt aagcattggg atgatttgtg gaagccggaa ttaaaagaca
    68161 gtttgatgct gattgacggt gcacgagaag ttatggggct ttctttgaat agtttaggct
    68221 attctttgaa tagtaagaat atggcacagt tgacagaagc agcaaataaa ttgaatcatt
    68281 taacaccaaa tgtcaaagca atcgtggcag atgagatcaa aatgtatatg atcaatgaag
    68341 aagcctccgt ggcagtgaca ttttctggcg aagctgccga tatgatgtgg gaaaatgaac
    68401 atttacatta tgtgatccca ccagaaggat ccaatctttg gtttgataat atcgttatgc
    68461 caaaaaccgt gaaaaataaa gaaggtgcct atgatttcat caactttatg ctagaaccgg
    68521 aaaatgcggc acaaaatgca gagtatattg gttattctac accaaatgaa gaagcaaaag
    68581 cgttattgcc aaaagacatt tcgtcggacg aacaattcta tccatctgat gacacgatct
    68641 cccatcttga agtctacaaa gatctaggat cgaaatacct aggcatctat aatgacctat
    68701 tcttagaatt caagatgtat aggagataat ggcaaaactc agtaagaatg gggacttatc
    68761 aacgttctaa agagctaaaa atcgtccttt ttctatctga gttttccaat cttctaccaa
    68821 aagcggatga aacgccgctt tttttgtgtt attttatttt ttctaccacg tttttctacc
    68881 agagaaaagt ggacaaaacc attgtaaaat agggcccgag acgggctagt aaatctaaga
    68941 ttgaaaaagt tacagaagga taaagtaatt ggaaactatg caaatatact taacccttct
    69001 gtaatctatt aataaaacta atatatgaat ccagttcaat atttgtagct atgaaaaaac
    69061 ataaaaatca tattagataa aaactaaatt ggtagataaa ttattttgtc agatatctga
    69121 ggcaaatatt cgactatttg tgtttcaaag taaaaattgg aatacaaata tagaatagac
    69181 atattgcagc cgatagttta ttatgctttt agacacatta aactggagga aaaaatgaaa
    69241 aagagcgcta aagatcgagt ttttgatgag ctgatacgac taacagggaa tatgtcttct
    69301 gtcactgccg agaaaatttc gaagaatcta ggtatctctc gtcagaatgc cagccattat
    69361 ttgacgagat tagtggaaga taaaaaggtc gagaaattac cgggaaagcc tgttttgtgg
    69421 aagcctttag atgaatatgc ggttgtagat aatacggagc aaataaacga agcctttcat
    69481 tctgtagttg gtcatgacgg aagtctgaga gaggtcattc aaaaatgtat agccgctgtc
    69541 aaatatccgc ctaacggact cagtgtatta ataaatggag ccacgggtgt tggaaaaagt
    69601 tttttagcca caaaaatttt tgaatacgct atccatgagc aaattattga aaaagatgca
    69661 ccatttgcta tcttaaactg tgccgattat gcggacaatc ccgaactgct ttcggcaaca
    69721 ctgtttggat acaagaaggg ggcctttacg ggggctgaaa aggataccga tggtttattg
    69781 gcaacggcca acaacggcta tctttttctc gatgaggtcc atcgcctatc gaaagaaaat
    69841 caagaaaagc tctttctatt tattgatact ggaaactatc gtccagtcgg tgaaaatgta
    69901 aattggcatt ctgctaaggt tcgttttatt ttcgccacga cggaaaaagg tgaaaattat
    69961 ttattagaca cctttgatcg gcgtattcaa attagtgtta tgttgccaac ttttgaagat
    70021 cgccccattc gagaacgatt agagctgatt caattatttt ttcaaaatga agcggatgtt
    70081 ttacaaaaag atattattgt ttcaagagaa gccttgtctt taatgttatc tcatcccttt
    70141 agtgggaata ttggtaagct gaagaatatc attaaaatca gttgtgcgga tgtgtatagt
    70201 cgaacacaag aagaaccatt aacgattggg aaaaatgaga tcttgattca gttaaatatg
    70261 ctggaatccg aagtagaaag cttgccgatt gctagtttat tgataaagaa aaatcaaaag
    70321 ataaaaacgg atcgaatcaa accgtttgat tatgattcga ttttggaaaa aattactcag
    70381 ctgataaatg aaggtaattt caatgaaaat ttttcttcga taaagaatga agtacagcat
    70441 ttggtgcatc acattgataa ggattctttt ttatcaatga gtagaaaatt gatgattcag
    70501 atgtttagaa aggtctgggt atccgtttta ggaaaaacgt acggattgac gacgagtgga
    70561 cctgttgcag aaattattag cagatcctat gtcatttccc caagctataa gacagactta
    70621 accgggataa atcgtacatt agctgcaaat ctacctcgga cgacttactt atgtaatcag
    70681 ttcttggagc atttaccacc gctctcagaa aaggacgaga actttctaag actgcttatt
    70741 gtcgtgactt tatctgatta tgtagatgaa tccatcgagc tgaagggcct gttgcttgct
    70801 cacggagata gtacggctag cagcattcaa gcagtagtga atcaattatg cggcaattat
    70861 gtttttgaag cgttggatat gccagtagag agtaatgtgt cagagattgt cgaatcgaca
    70921 aggaatttcg tgaaacggca agtgaataca gatagtctag tgctgatcgt agatatgggc
    70981 tctttaaatc aaatttacac acagatcaag aacgatttaa aaggggagtt acttcttctg
    71041 aataatttga cgacttccat tgcacttgat attggcttga agatgacgat gaatgcgcca
    71101 tttaaacaga ttgcggagga tgcagcagac aagtattcaa tcaatgttca atactttgaa
    71161 ggcttttctc aaagtagcaa cattatcatt tcctgtatgt cgggtttggg gatttcggac
    71221 aaattgaaag atgtttttcg tcatcattta tcggatgaat ccatcgaagt tttcacgaag
    71281 gactacagag aactacgtga attaatcgac caaaatgatg aaacttattt tgacaagaca
    71341 aaattggtca tcacgacttc ggatcttcca cgttcctttt caattcctaa tattaatgtt
    71401 tatgacatct tagacggtga tggagcagcc aatttggagc atattttgag tcctgaaatg
    71461 aagccggcag aatttaaccg cttgattcag gagctagtaa aattcttttc gttggaagga
    71521 attgcggacc gtttgaactt tctgaaccca acgatcgtga tgagtgaagt ggaaacagtc
    71581 atttctaaat atgaaaatta ctatcatttc actttaaatg gccgtgtaaa actaaatctc
    71641 tatatgcata cggcattgat gatggaacgt ttattcttgg ctcggacaga aaaatctgaa
    71701 gaaccgaatt atgatttgaa accagatgaa gatgaatttt attatgtttc tagaagtatt
    71761 tttcaatcaa tggagatgaa atataatttt aaagtcaatg cttatgagct ttcgctgctc
    71821 tatgaattat taggaccgta tattcaaaaa taaagtgtct aaaaaagtgt ctaatctgtt
    71881 ttttttaggt tagacacttt ttttaatgtg aattaacgtg attaaaccaa tgtttttaaa
    71941 agttggcaca ggaattgctt atataaaagt gaacagaaag aaaggaatta accatatgaa
    72001 gaaaattcta atagcgactc acggtcactt agcttcaggc tttcttagca gtattcaatt
    72061 actgacgggg aaggttcaag agattacggt gattaatgct tatgtggatg agtgtgattt
    72121 tgaacaggag ttgaatgtat ttgtaaacca ggtgaacgag caggatcaga tttttgtttt
    72181 taccgattta tttggcggaa gtgtcaatca gaagattacc aaaacctttt tggagaaaga
    72241 gattgctgta actattttgt ctggctttaa tctgccaatt ctgctcgaaa tagtattggc
    72301 aaccaaggaa ttaacaaagg aggatattca ggaactggta aataaatgcc aacaggaatt
    72361 aaagatgacc attcttgatg aggtacatgt agtaaatgaa gaggaagatt ttttttagga
    72421 ggaagaaaaa tgattaaagc agtgcgaatt gatgaacgat taattcatgg acaagtagcg
    72481 atgacttgga caaagagtct aaatttaacc gggttagtag tagcaagcga tgaggcggct
    72541 accaacgata ttcaaaagat gacattgaag atggctgcac catcgaatgt caaagtcatt
    72601 attaaaacag ttgatgacgc gattcatttg ttaaaagatc cgcgggcgaa ggacatgcgc
    72661 ttgatggttt tagtccctaa tgtcaaagat gcggtaaaag ttgcgaaagc tttcccaacg
    72721 gaaatcgaat taatgaacgt ggggaatgct gggaaaatga caccgccatc ggaagataag
    72781 aaaaccttat caaaggaagt gatgttaacg acgtcagaaa ttcaagactt aaaagaatta
    72841 gttgaacttt atccagatac cgtttttcag cctacaccgg cgatggaaaa acgttcagct
    72901 atctctattt tgagtgattt ttagaaaaag gaaggagaaa aattatgtat gatgcatttg
    72961 tagtcgcttt ggccgttttt attggaattg ctggacatga aatgttcggg atgtccatgc
    73021 tgagtcgtcc gattgttgtt gctccgttag caggagcgtt gatgggggat ttgcagacgg
    73081 ggttgattgt cggggcgtca ctagaagcca tctttatggg tgtcgtcaat atcggaatct
    73141 ctagtacggc ggagccttct ttggctgctg ggctggctac agcgttcgca atcaaaatgg
    73201 gaggaaacgt ggatgcgatt attcccattg tctttccact agcagtatta ggattacagt
    73261 taatgaatat gattttttct tttgtatgtg gaccgatggc tgctaaattt ttcaaatttg
    73321 caaaatctgg tgatcaaaaa ggaatcatca atctgcactt ctttatgtgg tttgtccact
    73381 atgcgcttta tgctcttatt ccattctttg ccgttttatt tggagcagat gttgtcaaaa
    73441 gcgtcttgga taatattcca gaggtcatca tgaacggatt aacagtcgct ggaaatctgc
    73501 tgccggcagt tgggatggcg atgctgttac ggatgctttg gagtaaaaat attgctgtgt
    73561 ggtttttctt tggggccgtt gttatggcgt atctcgagtt accattgatt gcaattgccg
    73621 cagttggaac gattattgca attgttatag cccaaagaga tctacaacta aaaaatatat
    73681 ctgttcatgc agttcaaagc gatgctgctg taaatgcaac gcttgaccaa gaagaggagg
    73741 atttctttca atgaaattag ctgatggaat ttctaaagat gagaaaaaat tggttcggaa
    73801 gatgttttgg cgctcgatga ctatgtatgc ttcggtgaat ccggtgacga tgggcggcgg
    73861 tggtttcgcc tattctatga tgcccttcat taatcatttc tacaaaagtg aagaagatcg
    73921 agctgcggcg ctggaacgtc atacacagta tttttcgacg accattcctt gcgcgtcctt
    73981 tgtcatgggg attgcggcat cgatggagaa ggaaaattct gaaaaagctg gttttgacgt
    74041 ggaaagtatc aacgcgatca aaacgagttt gatgggacca ttggctggaa ttggcgattc
    74101 attgttctgg ggagtttggc gtgtcgttac cgcgggacta gcgatcaact tagccaatgc
    74161 cggaaatatt ttggcaccaa ttatcttttt agttatgttc aatataccta attacttgat
    74221 gcgatactat ggcggcgttt taggctattc attaggctct aaatatattg aaaagctgta
    74281 tgcgaacggg ttaattgaaa ttctgacaaa agctgctagt atcttgggtc tgatcatggt
    74341 aggtgcaatg acgatcagta tggtcaaatt caataccgtt ttgagctttt caatgaaggg
    74401 cgaagaacta atgaatgtcc aagaaatcct cgatagtatt tttaaaggaa ttgtaccgtt
    74461 attggtaaca ttgagctgtt tcaaattact agataaaaaa gtcggcgtca tcaaaattat
    74521 ttttggaatg gtcgtcttgg gaattttatt gtcattactt ggaatcgcat agtaggaagc
    74581 tgcagctact aggctgcagt ttttttagca aggagggaaa agatgactat taatgaagta
    74641 atttattcac ccaaacaccc attttttaat gctgagttaa acgacggaaa tgtgactact
    74701 tcaaacgaat acagctacta tcggaatatt cctgatgtaa agggattgac cttcgatgta
    74761 agtaatcaaa agacattagc aaaccttgat gtttgcggga cgattcaaac gttgaccttt
    74821 tatcgagaaa atttactaac tgaggaaaaa ccaggtgttt gggtcaataa acagctctct
    74881 caaaccaatt cgttatcctt aaaggttgaa gtaaacggga ttgtctacca tttgtcagaa
    74941 tcggatcatc gtattgaagt cgatgtgatt caagactgtt tgcctagata tattcatcat
    75001 tattcgacgt ttaaggtcat tgtagtatcc tttgctccca tactggctaa taagcgttta
    75061 agtatgcttg ttcaacaggt ttacattgtg aatgaaacac acgaaagcct tcaagtttca
    75121 gtgaaagatg tgccttcgta tcaagagaag tattcggatc agcaaaatgt gttgatttct
    75181 caaaaaggaa ataaggacac gatcgcacaa caggaagtgg caagcttcgc cattgctttt
    75241 gtggacccaa atgcttttga agaagtaatg acttttcagg aatctgatat cgaaaaatgg
    75301 ctgaaggaaa cattgaacta ttttgctcag ctatttgggc agctgtcaat gccaaacgat
    75361 agagttgtcc atctctatcg tcgtgcactt tatcaaagct tttctagttt cggtatgaat
    75421 cgacagggac aggtcgtcgg cagcaattgg ggcagctatc ctgctactaa tcgaatttgg
    75481 aataaggata tgtattacgc atcattgcca ttcaccatgt ttgaaccgga gttatgtcaa
    75541 aaaacggttc tatggtttga tcaatatggc gttaagtttc ctggttcaaa atttacagga
    75601 ggtatcaatc attctctgag taattcgtta tccagtgcgt tactggctag tctttatttt
    75661 gaacatactg gggatacaaa attctttgag cggtgtccgc aggttctact gaatgcttca
    75721 agaattattg aggacatatt agctcaacgt cggtcagggg aaccaatgct ttttccatct
    75781 gtttggttat cagacgcctt tgcacttgga aagtatcata ccggcagcaa tctttgtctt
    75841 tggaaggcct gtattggact tgcagaaatt tttgaagccc tagaacaact gagtcttagc
    75901 aagcgataca aaaacattgc ctgtaaacta aaggaatcaa tttgtaaaca aatgactatc
    75961 gatggtcctt ttggtgagca atttttggag ggaatcggtg atgaggagaa aacaacctat
    76021 tcagtgaagc actatcaaaa accgattctt gagcaagggt taatcttttt aagtgatgtg
    76081 attgtggatg gaaaaattaa tttattgatg catgatggag aagaatcaga cactacgttg
    76141 atgccttttt atcaattttt agataataaa gatcatttat atcagaacac catggccttt
    76201 tctgctagtt ctttcaatcc aacctatagc gaggaaatca aaggcatcac ttggggatta
    76261 gaatccggcg ccacttttcc tggctttatc actgttttaa tgagcgagct gcacaaccct
    76321 caagctttcg taacccgatt agaagaacta atttgtttgg cagatttaga cggttcgtgg
    76381 tggtggtggc cgtatcgctt agaggctcaa caaggagaag tcgtgcgaga ctttggctgc
    76441 ggcaaatgcg gctgggcatc gggtcttttt gtcagcctaa tgatcagtca atatttcgga
    76501 ttgaaattca aaaaaggagt attgcaaatt gatcctgttg ataatttaac gttttcttgg
    76561 aagaatttga agttcggtca agccttcatt tcgattgaat gtacgcaatc cgagttgagt
    76621 atttcaaatt tgtcagaaaa gccattgaaa ataagtgata cgaaaaaaat tcttcacgta
    76681 gaaaagggaa aaacgattca cattcaaagg agaaaaagat gagtatacta aaaaatatta
    76741 cccaatttaa aaaaatccag agtcgagcaa tcactcccga aaatccaaca ggcgagaaag
    76801 gtaaagctgc gatgttgcca agcgagctag gaccaagccg caaaggtcgt ggcgcaattc
    76861 cgctgacttc tggtgaagaa actgtgattg cggatattac tggaacggga ataattcgtc
    76921 atatgtggat gacgattcgt gatcgaacag aatttggcag ttttgtttta cgtgatttaa
    76981 tcttaagaat ttattgggat gggtcggata gccctgctgt tgaaagtcct ttaggggatt
    77041 tcttttgtaa tgggtttgga gagcgttgcg aggtgaactc gttgccgata gtggttaatc
    77101 ctacaggggg ctttaattcc tatttcgaaa tgcctttcca aacatccgct aaaattacga
    77161 tcgagaattt acatccgaag gatatgtcct cattttttta tacgataaat tacgctttgg
    77221 tggacgaatt ggaagaaaat agcctatatt ttcacgcaaa ttggcatcgg gaacgcatta
    77281 ccaaattaca ggaggattat aagttaattg atcgattaaa gggtcaaggc tattacgtgg
    77341 gtacttacct tgcgcttacc tgcctagaac gttattggtg gggcgaaggc gagtttaaat
    77401 tctacattga tggggatagt gagtatccga ccgtaacctc aacaggttca gaagattatt
    77461 ttggcggggc gtgggccttt catgagtacg atgagttcgg acgtccacat gcgaaaaact
    77521 attcaacagc ctttttaggg tatccttacc aatcaaatag agatgccaca agagatcgtt
    77581 ttgaaacggg aaaattgaat gccgttcatg cctttggtga cgacgcaatg ccacgacatg
    77641 gtctttatcg ttggcatctt caagacccca tctgttttac cgaagattta tcggtcactt
    77701 tacaacaaat cggcaacgat gatgttcgct tatttgagcg gcaagacgat gttgctagtg
    77761 tcgcttactg gtattcaaca gaagcttcag gaaatactaa acgtcatttc actgatcgag
    77821 aacgcttgcc gcgttaatga agtgtttttc acatattcga agggaggaac ctatgatgat
    77881 atatggggca attgaagctg gagggactaa atttgtctgt gcagtcggta ctgaggaatt
    77941 aacgattttg gaaagagtca gttttcccac aacaactcca gttgaaacaa tgaagcaggt
    78001 cattgatttt tttaaacctt ttaaggaaca attggcaggc atcggggtcg gctgctttgg
    78061 tccaattgat attcaatgca aatcaacgag tttcggtcat atcacatcaa caccaaaact
    78121 tgcttggcaa aatttcaatt ttgtaggaac gctaaagcaa tatttcaata ttccgattga
    78181 atggacaaca gatgtcaatg cggcttgcta tggtgaatat gttgctggtt ctggcaaagg
    78241 gttagcaggt gttgcatact tcactgtggg aacaggtatc ggaggtggtg ccttgttcaa
    78301 tgggactttc gtggaagggt tcagtcatcc ggaaatgggg catatgttga tcaagcgaca
    78361 tccaaaggac aatttttcag gaaactgtcc cttccaccaa gattgtttag aggggatggc
    78421 agcaggacca gcgattgaaa aacggttggg tgtaaaaggc cagaatttat tggctgacga
    78481 ttcgttttgg cagattgaag ccttttacct tgcccaatgt gcatacaata caacactgat
    78541 gttttcgcca gatagaatta tttttggcgg tggtgtaatg aaacaagaac acatgaagaa
    78601 aaaagttcag gataaatttg ttgagcttat aaatggatat gttgaaatac caccaattga
    78661 tagctacatt attacgcccg aattaggtga taatgccgga ataatcggcg gattagcatt
    78721 ggccagaaaa gcggtaagaa ataagcaacc ataattaatg aatgtttcat tgatactcat
    78781 gtcaaaagta tttaaccgag taatcaaatg atcaaatgaa gttggataaa gcgaaaatga
    78841 attcaaataa aaaggctcta agtcaaatcg tgttgttttc attaaatcac tgaaaggaaa
    78901 tgcttctgcg tttccttttt tctttttgtt gtgcgctgta taccttgagg atattcactg
    78961 gtggcgtaca cttaaatagt aatatattgt gttactttat gtctgtggta atagttatag
    79021 ttgcttacca atgtatgttc atttttcgaa atgttgactt cataatcctt ggcttaatgt
    79081 ttgaattttt ttggtgatag attttttcag ccgtataaaa taaatcgtaa ttctattata
    79141 gagagttgtg attataaaaa gatagcaaga aaaaaaaaga aagtttctat acattttaaa
    79201 tattttattc ctatttctat tgctaaaaaa gtagaaattc tatatttacc tttaactctc
    79261 gcttctcttg caaatgtagt tagc
//
LOCUS       DACDCV010000005        63740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDCV010000005 DACDCV010000000
VERSION     DACDCV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.005"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV041_000343"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV041_000343"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8381164.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV041_000344"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV041_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8381165.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV041_000345"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV041_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8381166.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV041_000346"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV041_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8381167.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV041_000347"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV041_000347"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8381168.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV041_000348"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV041_000348"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8381169.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV041_000349"
     CDS             complement(8053..8523)
                     /locus_tag="IV041_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8381170.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV041_000350"
     CDS             complement(8569..8994)
                     /locus_tag="IV041_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8381171.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV041_000351"
     CDS             complement(9061..9330)
                     /locus_tag="IV041_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8381172.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV041_000352"
     CDS             complement(9341..10936)
                     /locus_tag="IV041_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8381173.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV041_000353"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV041_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8381174.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV041_000354"
     CDS             complement(14489..15049)
                     /locus_tag="IV041_000354"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8381175.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV041_000355"
     CDS             15402..16376
                     /locus_tag="IV041_000355"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8381176.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV041_000356"
     CDS             16546..17946
                     /locus_tag="IV041_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8381177.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV041_000357"
     CDS             complement(18024..18746)
                     /locus_tag="IV041_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8381178.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV041_000358"
     CDS             complement(18825..19331)
                     /locus_tag="IV041_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8381179.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV041_000359"
     CDS             19454..21253
                     /locus_tag="IV041_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8381180.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV041_000360"
     CDS             complement(21321..22895)
                     /locus_tag="IV041_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381181.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV041_000361"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV041_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8381182.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV041_000362"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV041_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8381183.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV041_000363"
     CDS             complement(24672..25310)
                     /locus_tag="IV041_000363"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8381184.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV041_000364"
     CDS             complement(25311..25961)
                     /locus_tag="IV041_000364"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8381185.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV041_000365"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV041_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8381186.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV041_000366"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV041_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8381187.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV041_000367"
     CDS             complement(29104..29844)
                     /locus_tag="IV041_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8381188.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV041_000368"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV041_000368"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8381189.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV041_000369"
     CDS             complement(31215..32153)
                     /locus_tag="IV041_000369"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8381190.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV041_000370"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV041_000370"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8381191.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV041_000371"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV041_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8381192.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV041_000372"
     CDS             complement(35313..35618)
                     /locus_tag="IV041_000372"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8381193.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV041_000373"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV041_000373"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8381194.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV041_000374"
     CDS             complement(36724..37008)
                     /locus_tag="IV041_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381195.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV041_000375"
     CDS             complement(37026..37916)
                     /locus_tag="IV041_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8381196.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV041_000376"
     CDS             complement(37936..38154)
                     /locus_tag="IV041_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381197.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV041_000377"
     CDS             complement(38124..39824)
                     /locus_tag="IV041_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8381198.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IV041_000378"
     CDS             40091..40864
                     /locus_tag="IV041_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381199.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV041_000379"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV041_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8381200.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IV041_000380"
     CDS             complement(41688..42659)
                     /locus_tag="IV041_000380"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8381201.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV041_000381"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV041_000381"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8381202.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV041_000382"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV041_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8381203.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IV041_000383"
     CDS             complement(45614..46438)
                     /locus_tag="IV041_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8381204.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IV041_000384"
     CDS             complement(46411..47091)
                     /locus_tag="IV041_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8381205.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IV041_000385"
     CDS             complement(47114..48052)
                     /locus_tag="IV041_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8381206.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IV041_000386"
     CDS             complement(48297..49148)
                     /locus_tag="IV041_000386"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8381207.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV041_000387"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV041_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8381208.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IV041_000388"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IV041_000388"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8381209.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV041_000389"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV041_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8381210.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV041_000390"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV041_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8381211.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IV041_000391"
     CDS             complement(56026..57267)
                     /locus_tag="IV041_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8381212.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV041_000392"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV041_000392"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8381213.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IV041_000393"
     CDS             complement(58294..59178)
                     /locus_tag="IV041_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8381214.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IV041_000394"
     CDS             complement(59956..61242)
                     /locus_tag="IV041_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8381215.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IV041_000395"
     CDS             complement(61235..62503)
                     /locus_tag="IV041_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8381216.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IV041_000396"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IV041_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8381217.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IV041_000397"
     CDS             complement(63276..63722)
                     /locus_tag="IV041_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381218.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT        17608 a        13848 c        11418 g        20866 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc
//
LOCUS       DACDCV010000006        63356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDCV010000006 DACDCV010000000
VERSION     DACDCV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.006"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(33..431)
                     /locus_tag="IV041_000398"
     CDS             complement(33..431)
                     /locus_tag="IV041_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8381219.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(428..1201)
                     /locus_tag="IV041_000399"
     CDS             complement(428..1201)
                     /locus_tag="IV041_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8381220.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1198..1881)
                     /locus_tag="IV041_000400"
     CDS             complement(1198..1881)
                     /locus_tag="IV041_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8381221.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2048..2935)
                     /locus_tag="IV041_000401"
     CDS             complement(2048..2935)
                     /locus_tag="IV041_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8381222.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2932..3801)
                     /locus_tag="IV041_000402"
     CDS             complement(2932..3801)
                     /locus_tag="IV041_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381223.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3938..5224)
                     /locus_tag="IV041_000403"
     CDS             complement(3938..5224)
                     /locus_tag="IV041_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8381224.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5497..6075
                     /locus_tag="IV041_000404"
     CDS             5497..6075
                     /locus_tag="IV041_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8381225.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6075..7004
                     /locus_tag="IV041_000405"
     CDS             6075..7004
                     /locus_tag="IV041_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8381226.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7001..8737
                     /locus_tag="IV041_000406"
     CDS             7001..8737
                     /locus_tag="IV041_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8381227.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8800..9636)
                     /gene="pheA"
                     /locus_tag="IV041_000407"
     CDS             complement(8800..9636)
                     /gene="pheA"
                     /locus_tag="IV041_000407"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8381228.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9633..10142)
                     /locus_tag="IV041_000408"
     CDS             complement(9633..10142)
                     /locus_tag="IV041_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8381229.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10135..11430)
                     /gene="aroA"
                     /locus_tag="IV041_000409"
     CDS             complement(10135..11430)
                     /gene="aroA"
                     /locus_tag="IV041_000409"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8381230.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11443..12534)
                     /locus_tag="IV041_000410"
     CDS             complement(11443..12534)
                     /locus_tag="IV041_000410"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8381231.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12551..13717)
                     /gene="aroC"
                     /locus_tag="IV041_000411"
     CDS             complement(12551..13717)
                     /gene="aroC"
                     /locus_tag="IV041_000411"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8381232.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13719..14783)
                     /gene="aroB"
                     /locus_tag="IV041_000412"
     CDS             complement(13719..14783)
                     /gene="aroB"
                     /locus_tag="IV041_000412"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8381233.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14816..15838)
                     /gene="aroF"
                     /locus_tag="IV041_000413"
     CDS             complement(14816..15838)
                     /gene="aroF"
                     /locus_tag="IV041_000413"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8381234.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16497..17471)
                     /locus_tag="IV041_000414"
     CDS             complement(16497..17471)
                     /locus_tag="IV041_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8381235.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17452..19335)
                     /locus_tag="IV041_000415"
     CDS             complement(17452..19335)
                     /locus_tag="IV041_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8381236.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19353..21578)
                     /locus_tag="IV041_000416"
     CDS             complement(19353..21578)
                     /locus_tag="IV041_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8381237.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21693..22727
                     /locus_tag="IV041_000417"
     CDS             21693..22727
                     /locus_tag="IV041_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8381238.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22794..23807)
                     /locus_tag="IV041_000418"
     CDS             complement(22794..23807)
                     /locus_tag="IV041_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8381239.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23885..24073)
                     /locus_tag="IV041_000419"
     CDS             complement(23885..24073)
                     /locus_tag="IV041_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8381240.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24513..24641)
                     /locus_tag="IV041_000420"
     CDS             complement(24513..24641)
                     /locus_tag="IV041_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8381241.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24644..26017)
                     /gene="rlmD"
                     /locus_tag="IV041_000421"
     CDS             complement(24644..26017)
                     /gene="rlmD"
                     /locus_tag="IV041_000421"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8381242.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26128..27183)
                     /locus_tag="IV041_000422"
     CDS             complement(26128..27183)
                     /locus_tag="IV041_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8381243.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27193..28623)
                     /gene="gatB"
                     /locus_tag="IV041_000423"
     CDS             complement(27193..28623)
                     /gene="gatB"
                     /locus_tag="IV041_000423"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8381244.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28620..30092)
                     /gene="gatA"
                     /locus_tag="IV041_000424"
     CDS             complement(28620..30092)
                     /gene="gatA"
                     /locus_tag="IV041_000424"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8381245.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30092..30397)
                     /gene="gatC"
                     /locus_tag="IV041_000425"
     CDS             complement(30092..30397)
                     /gene="gatC"
                     /locus_tag="IV041_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8381246.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30503..32539)
                     /gene="ligA"
                     /locus_tag="IV041_000426"
     CDS             complement(30503..32539)
                     /gene="ligA"
                     /locus_tag="IV041_000426"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8381247.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32556..34790)
                     /gene="pcrA"
                     /locus_tag="IV041_000427"
     CDS             complement(32556..34790)
                     /gene="pcrA"
                     /locus_tag="IV041_000427"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8381248.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            34945..36504
                     /locus_tag="IV041_000428"
     CDS             34945..36504
                     /locus_tag="IV041_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8381249.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36615..38546)
                     /locus_tag="IV041_000429"
     CDS             complement(36615..38546)
                     /locus_tag="IV041_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8381250.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38564..39478)
                     /gene="pfkB"
                     /locus_tag="IV041_000430"
     CDS             complement(38564..39478)
                     /gene="pfkB"
                     /locus_tag="IV041_000430"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8381251.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39475..40227)
                     /locus_tag="IV041_000431"
     CDS             complement(39475..40227)
                     /locus_tag="IV041_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8381252.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40386..40595)
                     /locus_tag="IV041_000432"
     CDS             complement(40386..40595)
                     /locus_tag="IV041_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381253.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40606..41934)
                     /locus_tag="IV041_000433"
     CDS             complement(40606..41934)
                     /locus_tag="IV041_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381254.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(42299..42886)
                     /locus_tag="IV041_000434"
     CDS             complement(42299..42886)
                     /locus_tag="IV041_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8381255.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(42969..44219)
                     /gene="clpX"
                     /locus_tag="IV041_000435"
     CDS             complement(42969..44219)
                     /gene="clpX"
                     /locus_tag="IV041_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8381256.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44466..45755)
                     /locus_tag="IV041_000436"
     CDS             complement(44466..45755)
                     /locus_tag="IV041_000436"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8381257.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(45909..46841)
                     /locus_tag="IV041_000437"
     CDS             complement(45909..46841)
                     /locus_tag="IV041_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381258.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(46998..47657)
                     /locus_tag="IV041_000438"
     CDS             complement(46998..47657)
                     /locus_tag="IV041_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8381259.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47660..48685)
                     /gene="sppA"
                     /locus_tag="IV041_000439"
     CDS             complement(47660..48685)
                     /gene="sppA"
                     /locus_tag="IV041_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8381260.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48698..48988)
                     /locus_tag="IV041_000440"
     CDS             complement(48698..48988)
                     /locus_tag="IV041_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381261.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49397..51202
                     /gene="glmS"
                     /locus_tag="IV041_000441"
     CDS             49397..51202
                     /gene="glmS"
                     /locus_tag="IV041_000441"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8381262.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51274..52629)
                     /locus_tag="IV041_000442"
     CDS             complement(51274..52629)
                     /locus_tag="IV041_000442"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8381263.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52653..53822)
                     /locus_tag="IV041_000443"
     CDS             complement(52653..53822)
                     /locus_tag="IV041_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381264.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53819..54706)
                     /locus_tag="IV041_000444"
     CDS             complement(53819..54706)
                     /locus_tag="IV041_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8381265.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(54895..58587)
                     /gene="nifJ"
                     /locus_tag="IV041_000445"
     CDS             complement(54895..58587)
                     /gene="nifJ"
                     /locus_tag="IV041_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8381266.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58786..59307
                     /locus_tag="IV041_000446"
     CDS             58786..59307
                     /locus_tag="IV041_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8381267.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60006..60563)
                     /locus_tag="IV041_000447"
     CDS             complement(60006..60563)
                     /locus_tag="IV041_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381268.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60742..61353)
                     /locus_tag="IV041_000448"
     CDS             complement(60742..61353)
                     /locus_tag="IV041_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381269.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61466..61723)
                     /locus_tag="IV041_000449"
     CDS             complement(61466..61723)
                     /locus_tag="IV041_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381270.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62320..62598)
                     /locus_tag="IV041_000450"
     CDS             complement(62320..62598)
                     /locus_tag="IV041_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381271.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62632..62883)
                     /locus_tag="IV041_000451"
     CDS             complement(62632..62883)
                     /locus_tag="IV041_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381272.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17638 a        13719 c        11203 g        20796 t
ORIGIN      
        1 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
       61 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      121 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      181 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      241 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      301 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      361 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      421 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      481 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      541 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      601 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      661 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      721 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      781 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      841 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
      901 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
      961 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1021 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1081 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1141 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1201 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1261 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1321 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1381 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1441 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1501 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1561 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1621 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1681 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1741 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1801 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1861 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     1921 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     1981 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2041 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2101 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2161 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2221 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2281 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2341 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2401 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2461 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2521 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2581 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2641 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2701 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2761 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2821 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     2881 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     2941 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3001 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3061 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3121 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3181 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3241 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3301 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3361 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3421 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3481 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3541 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3601 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3661 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3721 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3781 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3841 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     3901 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     3961 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4021 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4081 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4141 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4201 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4261 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4321 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4381 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4441 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4501 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4561 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4621 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4681 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4741 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4801 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4861 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     4921 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     4981 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5041 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5101 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5161 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5221 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5281 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5341 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5401 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5461 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5521 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5581 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5641 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5701 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5761 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5821 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     5881 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     5941 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6001 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6061 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6121 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6181 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6241 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6301 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6361 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6421 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6481 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6541 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6601 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6661 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6721 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6781 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6841 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     6901 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     6961 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7021 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7081 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7141 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7201 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7261 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7321 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7381 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7441 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7501 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7561 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7621 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7681 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7741 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7801 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7861 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     7921 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     7981 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8041 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8101 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8161 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8221 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8281 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8341 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8401 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8461 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8521 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8581 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8641 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8701 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8761 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8821 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     8881 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     8941 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9001 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9061 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9121 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9181 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9241 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9301 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9361 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9421 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9481 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9541 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9601 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9661 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9721 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9781 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9841 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
     9901 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
     9961 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10021 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10081 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10141 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10201 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10261 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10321 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10381 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10441 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10501 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10561 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10621 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10681 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10741 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10801 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10861 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    10921 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    10981 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11041 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11101 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11161 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11221 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11281 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11341 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11401 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11461 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11521 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11581 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11641 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11701 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11761 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11821 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    11881 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    11941 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12001 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12061 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12121 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12181 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12241 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12301 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12361 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12421 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12481 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12541 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12601 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12661 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12721 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12781 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12841 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    12901 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    12961 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13021 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13081 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13141 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13201 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13261 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13321 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13381 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13441 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13501 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13561 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13621 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13681 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13741 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13801 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13861 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    13921 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    13981 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14041 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14101 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14161 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14221 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14281 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14341 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14401 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14461 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14521 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14581 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14641 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14701 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14761 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14821 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    14881 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    14941 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15001 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15061 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15121 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15181 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15241 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15301 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15361 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15421 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15481 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15541 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15601 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15661 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15721 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15781 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15841 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    15901 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    15961 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16021 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16081 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16141 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16201 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16261 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16321 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattga ttttattgaa
    16381 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16441 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16501 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16561 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16621 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16681 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16741 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16801 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16861 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    16921 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    16981 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17041 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17101 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17161 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17221 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17281 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17341 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17401 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17461 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17521 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17581 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17641 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17701 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17761 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17821 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    17881 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    17941 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18001 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18061 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18121 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18181 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18241 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18301 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18361 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18421 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18481 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18541 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18601 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18661 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18721 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18781 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18841 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    18901 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    18961 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19021 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19081 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19141 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19201 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19261 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19321 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19381 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19441 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19501 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19561 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19621 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19681 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19741 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19801 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19861 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    19921 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    19981 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20041 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20101 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20161 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20221 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20281 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20341 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20401 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20461 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20521 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20581 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20641 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20701 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20761 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20821 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    20881 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    20941 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21001 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21061 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21121 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21181 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21241 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21301 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21361 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21421 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21481 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21541 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21601 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21661 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21721 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21781 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21841 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    21901 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    21961 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22021 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22081 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22141 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22201 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22261 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22321 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22381 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22441 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22501 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22561 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22621 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22681 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22741 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22801 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22861 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    22921 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    22981 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23041 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23101 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23161 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23221 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23281 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23341 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23401 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23461 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23521 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23581 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23641 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23701 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23761 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23821 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    23881 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    23941 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24001 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24061 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24121 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24181 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24241 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24301 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24361 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24421 ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa
    24481 agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt
    24541 ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt
    24601 ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga
    24661 atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct
    24721 tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac
    24781 atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat
    24841 ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc
    24901 attttttcat aactttttct gcttttccga cttcataatg aacattttcg attttattat
    24961 ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct
    25021 tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca
    25081 caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt
    25141 gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct
    25201 gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg
    25261 ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag
    25321 cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca
    25381 tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt
    25441 cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt
    25501 gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct
    25561 ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat
    25621 tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct
    25681 tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat
    25741 acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct
    25801 gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg
    25861 cttttactac tagtatttct acttcttctc cagggagtgc attttcgata aataatggat
    25921 aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat
    25981 atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt
    26041 ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac
    26101 cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat
    26161 ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt
    26221 gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc
    26281 ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc
    26341 gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac
    26401 gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt
    26461 cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa
    26521 agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga
    26581 gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt
    26641 caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc
    26701 aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata
    26761 gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac
    26821 ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa
    26881 tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc
    26941 aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac
    27001 tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg
    27061 tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc
    27121 caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt
    27181 cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg
    27241 atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc
    27301 ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat
    27361 tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg
    27421 ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat
    27481 cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc
    27541 agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga
    27601 tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt
    27661 tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc
    27721 gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc
    27781 gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt
    27841 gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat
    27901 ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt
    27961 cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc
    28021 ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc
    28081 gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc
    28141 tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa
    28201 atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg
    28261 gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc
    28321 atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa
    28381 gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc
    28441 tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg gataacccca
    28501 gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa
    28561 aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt
    28621 cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca
    28681 aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat
    28741 ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag
    28801 ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca
    28861 ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag
    28921 actttttcaa aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg
    28981 tcataataac ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca
    29041 ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag
    29101 cgataaccgt agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca
    29161 atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca
    29221 gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc
    29281 acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc
    29341 agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa
    29401 ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt
    29461 tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt
    29521 ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta
    29581 cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca
    29641 ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta
    29701 cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga
    29761 tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat
    29821 gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca
    29881 gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct
    29941 ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca
    30001 aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt
    30061 tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga
    30121 ttgcgggtac tttgataaag ccgtcttctg tttccggcac atttttcaat aattcatcgc
    30181 ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg
    30241 acgtaaatgg aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt
    30301 tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt
    30361 tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag
    30421 ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa
    30481 tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg
    30541 ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc
    30601 cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc
    30661 cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag
    30721 taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa
    30781 gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt
    30841 cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg
    30901 caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta
    30961 aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga
    31021 ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta
    31081 ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta
    31141 ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg
    31201 catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga
    31261 tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat
    31321 gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa
    31381 cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct
    31441 tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc
    31501 ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga
    31561 tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg
    31621 gtgctttgat agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga
    31681 ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc
    31741 acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt
    31801 ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg
    31861 tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag
    31921 aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt
    31981 ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca
    32041 ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt
    32101 ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca
    32161 aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg
    32221 ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac
    32281 tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc
    32341 gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt
    32401 ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt
    32461 cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa
    32521 tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag
    32581 gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc
    32641 gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct
    32701 ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc
    32761 tctggctgtt tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta
    32821 ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt
    32881 cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct
    32941 tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct
    33001 tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact
    33061 ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct
    33121 tctaaattgt caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct
    33181 tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg
    33241 accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt
    33301 tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc
    33361 aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt
    33421 cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat
    33481 tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg
    33541 ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa
    33601 tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta
    33661 taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg
    33721 tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg
    33781 tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt
    33841 ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact
    33901 tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact
    33961 gcagcatctg gataatcttt ttcaaaatcc aagatgttct gcatatctgc accgcgccag
    34021 ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat
    34081 aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa
    34141 tggaatttgt tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta
    34201 ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca
    34261 gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga
    34321 ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat
    34381 tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa
    34441 gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata
    34501 cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc
    34561 gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg
    34621 ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc
    34681 gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct
    34741 tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc
    34801 ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt
    34861 tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa
    34921 attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag
    34981 actcagatat attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt
    35041 agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg
    35101 agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt
    35161 ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta
    35221 gagggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc
    35281 attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata
    35341 cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa
    35401 gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct
    35461 atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg
    35521 ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg
    35581 agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta
    35641 gtgatcttag gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct
    35701 ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc
    35761 ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc
    35821 gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt
    35881 cgattcgttt tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg
    35941 atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg
    36001 atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg
    36061 attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat
    36121 catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc
    36181 ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca
    36241 ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga
    36301 ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga
    36361 aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc
    36421 ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc
    36481 acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat
    36541 ctacactact tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct
    36601 cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc
    36661 acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt
    36721 aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact
    36781 aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct
    36841 gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa
    36901 ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt
    36961 gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt
    37021 gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg
    37081 cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca
    37141 ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc
    37201 agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta
    37261 attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa
    37321 ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat
    37381 gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat
    37441 cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta
    37501 gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg
    37561 tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt
    37621 gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct
    37681 tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct
    37741 agattgatca actcttctgg tttacggata ccatcactga caggacgatt gatcagttct
    37801 tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca
    37861 cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact
    37921 ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg
    37981 tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca
    38041 gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca
    38101 gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg
    38161 aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt
    38221 gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca
    38281 taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt
    38341 ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct
    38401 agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg
    38461 accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct
    38521 ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga
    38581 tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag
    38641 ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc
    38701 tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg
    38761 ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc
    38821 cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa
    38881 tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg
    38941 gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa
    39001 tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc
    39061 gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta
    39121 atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct
    39181 cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga
    39241 acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc
    39301 ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga
    39361 tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta
    39421 acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg
    39481 tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg
    39541 ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa
    39601 tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac
    39661 agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc
    39721 cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac
    39781 agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac
    39841 taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa
    39901 aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat
    39961 ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc
    40021 actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc
    40081 tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt
    40141 caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat
    40201 tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata
    40261 ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc
    40321 aaaaaaaaat aaaaaaccag tcagaaagac tggtttttta tttttttgtc tatcatcaag
    40381 ccgttttatc gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt
    40441 ctttccttat gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg
    40501 atgactgatc gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc
    40561 ggaaccatta ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa
    40621 agtaatcaat tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc
    40681 tttcttgaca tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc
    40741 agagagtcta tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata
    40801 gattcttaag gataaaagat actcttgata tttttcatct gttacctcta cttctagata
    40861 ttccctaata taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat
    40921 aactttatag cacatcatgc gtgatcgatt ctcgtcaacc caaggaaaat acgcctgagc
    40981 atttagtgtt ccgcgaacac gcctttcttt ataatctcct tgttggatat cataactttt
    41041 tgttacaaaa aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg
    41101 gacagattgg atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc
    41161 gacaccattg tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa
    41221 catttgattg tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac
    41281 ttcgttttct aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg
    41341 gatgatttga tgataatagc gactataata catttcccaa cacgaagtga aacataaacc
    41401 ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg
    41461 ttgtttcatt ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga
    41521 gatctgtttt gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc
    41581 atgagcaatc atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac
    41641 agagatagac aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc
    41701 gttctcgtca tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt
    41761 ttcttgaagc tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt
    41821 tggctggatc aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc
    41881 ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc
    41941 acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa actaaaactt
    42001 tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag
    42061 gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca
    42121 tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa
    42181 ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa
    42241 agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt
    42301 atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg
    42361 aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct
    42421 catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag
    42481 ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac
    42541 gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa tatgtctcga
    42601 tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa
    42661 catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg
    42721 gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt
    42781 tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt
    42841 ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta
    42901 acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc
    42961 tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac
    43021 aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca
    43081 aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg
    43141 cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta
    43201 ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag
    43261 atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg
    43321 ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca
    43381 ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata
    43441 cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc
    43501 gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg aggtggcaca
    43561 cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg
    43621 tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag
    43681 atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa tttaagcaaa
    43741 atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt
    43801 gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct
    43861 gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt
    43921 gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg
    43981 cgatcttgtc caatcacgta gttattaaat acttcaagaa tttctagtgg tttagggact
    44041 tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg
    44101 tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct
    44161 tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg
    44221 gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt
    44281 tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta
    44341 tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag
    44401 tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt
    44461 gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc ttttttcatt
    44521 gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg
    44581 ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga
    44641 ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca
    44701 ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca
    44761 ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca
    44821 tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc
    44881 gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga
    44941 tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg
    45001 tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct
    45061 tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga
    45121 ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa
    45181 cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag
    45241 ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact
    45301 aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact
    45361 tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc
    45421 actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt
    45481 tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt
    45541 aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact
    45601 tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta
    45661 aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca
    45721 tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt
    45781 cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa
    45841 ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact
    45901 gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa
    45961 tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc
    46021 agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca
    46081 gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc attcccgtaa
    46141 cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct
    46201 tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg
    46261 ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat
    46321 acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca
    46381 atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt
    46441 gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca
    46501 atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctagcatat
    46561 aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc ctcattttga
    46621 agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc gtccaactcg
    46681 taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc gtaagctcgt
    46741 tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg tgccactcgc
    46801 attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc tcctcttgct
    46861 catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt aaaaataact
    46921 gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt tcaactgtta
    46981 ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat cagattgatc
    47041 gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg attaaagact
    47101 gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca agcaccttct
    47161 ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac tcgaatacca
    47221 aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat aaaatacgct
    47281 agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga aaaattaagc
    47341 caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat acacaaaaga
    47401 tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt aggaaaatca
    47461 tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg ttgtttcttt
    47521 ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt ttgggaaaag
    47581 ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc cttattttca
    47641 tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt ggtgattccg
    47701 ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc ttcaatccct
    47761 ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc gtggaagaat
    47821 attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt aaagcatctt
    47881 caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca tcataaatcc
    47941 ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg ctgacgattg
    48001 ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct tcttcttttg
    48061 tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact gttgtatctt
    48121 ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc acaccaattg
    48181 agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa atatagtagc
    48241 caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt tctttacgaa
    48301 tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca ggagaattta
    48361 cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt tgttccataa
    48421 aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc gcagcaatcg
    48481 ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc aacacatttg
    48541 gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga tcgttttcct
    48601 gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca gcaatcagaa
    48661 ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt tttatgacga
    48721 tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc cgcgcatacc
    48781 cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct tgagcaactt
    48841 ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa aatattttcg
    48901 tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt aacattccca
    48961 gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat tactttctat
    49021 tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga cataaaaagg
    49081 acatttgaca aagaatttga tatataccaa tttatttttt attctttttt tgttgacaag
    49141 aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa aggaggatag
    49201 ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga gggtttatcg
    49261 aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa aaaatgtctg
    49321 caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat tcattataga
    49381 aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa atgtaacacc
    49441 aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag ctggtatctt
    49501 tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc aaaatttgaa
    49561 aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac gatgggcaac
    49621 tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg gacgcttcgt
    49681 gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt atttagcaaa
    49741 tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg aaactttcgc
    49801 gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca aaggttctta
    49861 cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta aaaataaaag
    49921 tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg caatggctat
    49981 gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta ccgtcacatc
    50041 agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt cattcgaagc
    50101 acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc tgaaagaaat
    50161 cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag aagacaatcg
    50221 tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa tctatatcgt
    50281 tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg aaaaattgac
    50341 acagattcct gttgaggttc atttagccag tgaatttggc tataacacac ctcttctttc
    50401 aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca gccgccaagt
    50461 gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg ttgctggttc
    50521 tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg aaatcgcagt
    50581 agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag ccaaagctat
    50641 cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg aattgagttt
    50701 agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag agttggcaga
    50761 agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg actacaacgt
    50821 ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag aaggttttgc
    50881 cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac cagtgatcgg
    50941 aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag aagtcgaaag
    51001 ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa gtgaccagct
    51061 ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac cgactcaatt
    51121 acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac gtaatttagc
    51181 gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat ctgacactgg
    51241 tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt cttctttgac
    51301 tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg cttctgccat
    51361 cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt ctgatcccat
    51421 ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg caccgttttt
    51481 atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg ctgcaagctc
    51541 tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg aaagcatccc
    51601 atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc cgccaaagtt
    51661 ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg taacgacatc
    51721 tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga cagtcgtaac
    51781 gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga taaacatgat
    51841 tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat cgccatcgcc
    51901 atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta aacgttctgg
    51961 atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc ccattgtata
    52021 aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg ctccgtttgc
    52081 tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta caaggaattg
    52141 agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt cagctgatgg
    52201 acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct cttggtcatc
    52261 tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg ctggattatg
    52321 agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat aagcaacacc
    52381 tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa gtcctgaaat
    52441 cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta acacgcgtgg
    52501 gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc ccagcttaaa
    52561 tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg ttccaaaata
    52621 tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt tgttctgctc
    52681 atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga gctgcttgtc
    52741 ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga atcagcagat
    52801 gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga agcagagcta
    52861 ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg cttgatactt
    52921 aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat acctgagaca
    52981 ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga gccgcttact
    53041 tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc tggtgttcct
    53101 tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc tctcactgtt
    53161 acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag cgcctgtatt
    53221 gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc agctactact
    53281 cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt aggactaacg
    53341 gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa cgtttccaca
    53401 tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg ttcgatcttt
    53461 gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt tccttcgccc
    53521 agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc ctctgcattc
    53581 aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata tccatggata
    53641 tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt ttgattatac
    53701 gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa gtacagcgca
    53761 atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc ttttgagatc
    53821 atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg tttttcttgt
    53881 tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat tcttgctggg
    53941 ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt tcttcagaga
    54001 caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg tgtcgagtcc
    54061 cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt gctactgcga
    54121 tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga ataaagatat
    54181 tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca atatactcat
    54241 ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg gacatatatt
    54301 ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg ctttttgatt
    54361 gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc ggctgaaaaa
    54421 tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa gacaaggtgt
    54481 gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca cctttcagaa
    54541 gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga taccagacaa
    54601 ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg gaaaacattt
    54661 gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa agcctctctt
    54721 ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc tgatatgctc
    54781 gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc gttttatttt
    54841 tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt tctgttattt
    54901 ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc tggcagctct
    54961 tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc tagtcttaac
    55021 gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta attctcccgt
    55081 cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt caaataaagc
    55141 ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt tcctcatgaa
    55201 atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt tcttgttttt
    55261 ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa cagcttcttt
    55321 tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac aaggcgtata
    55381 ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct tgatcgtttg
    55441 catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg tcatcgccat
    55501 cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag cgattgccga
    55561 agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt tatccaaaac
    55621 aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa aaccaatgtc
    55681 gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata gatcctttct
    55741 cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg cagcttgcag
    55801 ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc agtcttccat
    55861 caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac ttgccaaagc
    55921 ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat tatcttccaa
    55981 taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag gagaagcagg
    56041 agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca ttcgatcacc
    56101 gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc aagcgcccga
    56161 gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc ctttgactgg
    56221 atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt ctttctgttc
    56281 ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta cacacagacc
    56341 acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt ctgcgcctcg
    56401 catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat ctgctaaaaa
    56461 tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg tacagcgatc
    56521 actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag ccgttcctaa
    56581 aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga gttgatttcc
    56641 ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc ttttattgat
    56701 tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat gaagcagttc
    56761 ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt tatgtccata
    56821 gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa tgatgtcagc
    56881 taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac cgacttcttg
    56941 ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca agtaacgttt
    57001 taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat tcaataaaaa
    57061 aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag ctggtgtgtg
    57121 acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg tatctccaaa
    57181 acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa aatagccttg
    57241 cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg ctccaactgt
    57301 tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg tcaaatccag
    57361 actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta tggtaaaccg
    57421 ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga tttggtcggg
    57481 agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc gaagttcaga
    57541 atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc ctggttcttt
    57601 acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca cgaatgtttc
    57661 cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc cgtttgcatt
    57721 caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa caatgacttc
    57781 ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc gtaatcggtt
    57841 gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg ggttgatcgt
    57901 ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag tcggatgatt
    57961 gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca gaggagctaa
    58021 ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga atccatcaaa
    58081 gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag gtgctaaatc
    58141 catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc ttgcagccat
    58201 cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc gactagcaac
    58261 gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta tcatcaaaag
    58321 cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg atccatggac
    58381 cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt gaccgaatac
    58441 gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg aactgggtgt
    58501 tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt
    58561 gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt ctctcttatt
    58621 atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa aaatatttta
    58681 catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat cgttgccttt
    58741 ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga tatatcagtg
    58801 attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc gaatgctcct
    58861 aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg gtttgaagaa
    58921 tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt gatcaaattg
    58981 ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta tctcacaatc
    59041 agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca agacgagtgg
    59101 aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa gctgacagaa
    59161 caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc acatattcga
    59221 tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc aatagaggaa
    59281 ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtgggacaaa agtaaagaac
    59341 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    59401 ttcttggagt taaacgcttc tgtcccagcc tccttattcc gaataaatgg tgtccaaaag
    59461 cttgcatctg ccagattatc tcatttctta tgacaagcta agcaaattat ctccaccaaa
    59521 taatttattc atatttttga cttgcgcata ctaactaatt cttaaatttt catacaaaat
    59581 ttctcaattt cccttaacga gtaatattta tgtacggtga ttctcactag tatcccaatc
    59641 actttttaag ttatcccgat ttcaattcta cttcaaaaat agttgctttt atgtatgctg
    59701 ttagttgatt agatggtcat ttgttgagct aggtcgttta ttttaaacaa gttgtttctt
    59761 tagcttatac ctatctcgtc taccaaacgc aagatggcaa acacaaatgc atacgcatct
    59821 gtatttgcca ctttgattcg tataaagcta acaactatta actcctctca gctaatagtt
    59881 gctctctttt ttattcagat tcatttttgc tgatcacagc ctagtatttc aatggttttg
    59941 aacactaaac caaattgttc cattacaaca ttcactggct gatgaaatgg attagtagat
    60001 ttcgattatt caaagcgatt ttctattggt ggatcgtgtt cccaactaat aagacggatt
    60061 ccaaacctat cgtacagatc ttctaaacca cttataaatt ttgcacctat ttcaggtgtt
    60121 ttattgcgtg gatattctat tgttacgcca ttaaatggat accaattagg attaggctct
    60181 ctttttttct gtacatctcc catagaatag ttagcacttt ctgggacata atatattctt
    60241 cgaccatcct ctaattcatc taataagata tttcgtgatc gtctatcact tgctaaatgt
    60301 agacgccacg tgcgatacca tgccatgcta cctgcatctg cagaaaatcc atcaaataat
    60361 gcaacaattg tatcttctat cacaatcctt tccccatcta cttcatattc taatctaaaa
    60421 tcaaactccc ctcgggtaat ttctggtctt ggtggcttag gacccaatat tattctactt
    60481 aaaaccgcat tcgttcccag atgaaacaca gctataacaa caataatgat taatattttg
    60541 tttctatttg ataaatttct cattattcgc acatcacctc tacattctca catattagtt
    60601 cgtaatactc ctttaatatc aaaatatcta ctggtcgtaa taatattcgc tatgaattcc
    60661 tactactcgt ataagaaagg ccaaatcatc agcttttact tgctgatgat ttggcttgtt
    60721 tctatttatc gtaattgttt tctaatcacc aaaattgtta acaatcggtt cagatatttc
    60781 ccagttgatt aaggtaattc cgtattgtgt caaaacttct tgcgcttctc cgaaattaag
    60841 tacagttctt atgtatccac tttctcctaa tataaagtct acctctcttt taaaaccttc
    60901 ctcgagattt ctaagggcta tatgtggttt taatgacatt gattgtacat tttcccccat
    60961 ataatattgc gcacttcctc gactaaaata gattcctaga tcgtccccaa aaggtagatc
    61021 ttctctacca ctagctaatc tacttctcca tcttcgttct cttttcatag aacctgcgct
    61081 gaactcacac ccgttaaact cagctatgac agtatcttca attatgttaa tctgtccatc
    61141 tagttcgtat tcaatcctaa atggaaattc tccataggtg atttccggtt cgggtatttt
    61201 aggtgagatt aatgcaaaga aaggaaatat taagcttggc aacatcaaaa aaactcctcc
    61261 tatgatcaca attcccgatg taatccaaaa tataatttgt acaattctca ttgatccatt
    61321 gtattccatt ttattccccc tattactatt catatccgcc tatctatatc gatcttccaa
    61381 tgcagagata attgcttcca taccttgatt ttttactgag ttacctatgc gagatatcat
    61441 tctccactaa ggtgcatcat attgtctatc taacatgatt aattctccaa taggtgtata
    61501 ctcctccaaa aacaacattt aatgcatctc cctcgacaac ataagctacc atgtttgctg
    61561 tatatgtgtt tgagtctaat tcataagcag ataaatttac tgtcgctgga ttaaatataa
    61621 tcgcattcgt atttatagct actgaatttg ctgcttcagc accacctttt gaatgtccga
    61681 tcattgtgat ttcaatagta ggatttacgt ggacagccct caccgtttac cctgaccatt
    61741 atagaaatcg cttacttttt tgaactacgc aacaaaggat acaatgctcc taacttcact
    61801 gtctccattt ttgtccacta aggaatataa aaaataggca ttaattcatt taattcggtc
    61861 acaacacgtt accttttaaa tcattatact gtatctcata cattattatc aataaactat
    61921 tataaaaatg caaaaaatgc tttatcaatc tattttgtaa ttggtcataa tatcattgaa
    61981 atcataccct gtatcgattt ttcctatgaa tctcgtctat ttgtgattga cgaaaaagag
    62041 gcaagacaga cactggaatg gtaattttac ccactatttt gctccaacta tttgtaatcg
    62101 tacccacttt tgaaataccg agatgatcat tttaattgat atgctgattc ccaagcaaaa
    62161 ccccaagaaa ttaaatgctc tatcgcatct agcttcttgg gttttgctta tataaattcc
    62221 aacatttaaa ttttttatgc tgttcggtct cagaaatgcc gtttttgctt atatcacaat
    62281 atcaagatgt caaattattc gtgttgccga tgactttgat taattttttt caaattgttt
    62341 attaagacta atgctgtctt tttgcagata ccaatgttcg aactttttaa cattatcata
    62401 tgatcctgca agttttaact tcttaccatt agatgttatc aaagttataa ttaaccttgc
    62461 ctttttccca ttaggatctt ttattttatg aattttctgc aggtaatctc tcctaatggc
    62521 agctattttt acctcatcag tgctgataaa ccattgatca tccacataca tactgcctgt
    62581 tatgttgtgt ttttccattt cttccgagaa cgaaaagttt aaggctcgtt cttacttcca
    62641 aagttgaatg agtccatgga ttggacttag tgtgagaatt gagaacaaca ataagaatcc
    62701 atatcctccc gcaaacatcc ctaaggctat actgtctctc catccataag taggattaga
    62761 agagaaaaga gtcaccgttt gtaataatat gaaaaggaat aatccagcac aaacaccaaa
    62821 gataacaact ccaaacattg aatttttata caatgaccat ttatttatag gcttttcttt
    62881 cattttactt ccccctactg ttctgttcac acggaatgaa ttcaaagttc ataagccctc
    62941 ctctttgaac atatgaatac gacttgctaa ctatacttta aaaaagcttg ttttaagcgg
    63001 ttataggcga gttgtaatct ctattcatct cattagcaaa taacttgccg aaaagtctac
    63061 tgaaattaaa gcagatagtg gaagaattat ttagccagga tagacactat ttgtgaaaca
    63121 catgtcttta cttttgaaat tctatgatga aaagccttgg aatacagtag ctcttaattg
    63181 gtaatacttt ttccattata aataacctct tttcatttta tatttatccc ctctaaaaat
    63241 aatatctaat ctaatatttt aaacttatat ttccctcgct gtgctaatag agcattacta
    63301 aataaataga aactagaaaa aagcattacg cctgccagaa taaaccaatt atttgt
//
LOCUS       DACDCV010000007        60191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDCV010000007 DACDCV010000000
VERSION     DACDCV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.007"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV041_000452"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV041_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8381273.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV041_000453"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV041_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8381274.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV041_000454"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV041_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8381275.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IV041_000455"
     CDS             1642..2274
                     /locus_tag="IV041_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8381276.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IV041_000456"
     CDS             complement(2276..3181)
                     /locus_tag="IV041_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8381277.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IV041_000457"
     CDS             complement(3266..3919)
                     /locus_tag="IV041_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8381278.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IV041_000458"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IV041_000458"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8381279.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IV041_000459"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IV041_000459"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8381280.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IV041_000460"
     CDS             6168..7253
                     /locus_tag="IV041_000460"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8381281.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IV041_000461"
     CDS             7250..8314
                     /locus_tag="IV041_000461"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8381282.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IV041_000462"
     CDS             complement(8563..9366)
                     /locus_tag="IV041_000462"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8381283.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV041_000463"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV041_000463"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8381284.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IV041_000464"
     CDS             complement(10244..11251)
                     /locus_tag="IV041_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8381285.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IV041_000465"
     CDS             complement(11258..11902)
                     /locus_tag="IV041_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8381286.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IV041_000466"
     CDS             complement(12002..12760)
                     /locus_tag="IV041_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8381287.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV041_000467"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV041_000467"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8381288.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV041_000468"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV041_000468"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8381289.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV041_000469"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV041_000469"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8381290.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV041_000470"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV041_000470"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8381291.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV041_000471"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV041_000471"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8381292.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTSDYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IV041_000472"
     CDS             18158..19027
                     /locus_tag="IV041_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8381293.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IV041_000473"
     CDS             complement(19153..20052)
                     /locus_tag="IV041_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8381294.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IV041_000474"
     CDS             complement(20217..21041)
                     /locus_tag="IV041_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8381295.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IV041_000475"
     CDS             complement(21052..22347)
                     /locus_tag="IV041_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8381296.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IV041_000476"
     CDS             complement(22363..22842)
                     /locus_tag="IV041_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8381297.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IV041_000477"
     CDS             complement(22848..23876)
                     /locus_tag="IV041_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8381298.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IV041_000478"
     CDS             complement(23893..24999)
                     /locus_tag="IV041_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8381299.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV041_000479"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV041_000479"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8381300.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV041_000480"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV041_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8381301.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV041_000481"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV041_000481"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8381302.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IV041_000482"
     CDS             29970..30668
                     /locus_tag="IV041_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8381303.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IV041_000483"
     CDS             complement(30836..33475)
                     /locus_tag="IV041_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8381304.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IV041_000484"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IV041_000484"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8381305.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IV041_000485"
     CDS             35318..36493
                     /locus_tag="IV041_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8381306.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IV041_000486"
     CDS             36497..37138
                     /locus_tag="IV041_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381307.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IV041_000487"
     CDS             37135..38052
                     /locus_tag="IV041_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8381308.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IV041_000488"
     CDS             38049..38720
                     /locus_tag="IV041_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381309.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IV041_000489"
     CDS             complement(39261..39749)
                     /locus_tag="IV041_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8381310.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IV041_000490"
     CDS             complement(39950..40216)
                     /locus_tag="IV041_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8381311.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IV041_000491"
     CDS             complement(40216..40479)
                     /locus_tag="IV041_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8381312.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IV041_000492"
     CDS             complement(40472..40744)
                     /locus_tag="IV041_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381313.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IV041_000493"
     CDS             40955..41140
                     /locus_tag="IV041_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8381314.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IV041_000494"
     CDS             complement(41282..43105)
                     /locus_tag="IV041_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8381315.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IV041_000495"
     CDS             complement(43703..46408)
                     /locus_tag="IV041_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8381316.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IV041_000496"
     CDS             complement(46714..47661)
                     /locus_tag="IV041_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8381317.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IV041_000497"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IV041_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8381318.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IV041_000498"
     CDS             complement(48296..48658)
                     /locus_tag="IV041_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8381319.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IV041_000499"
     CDS             complement(48669..49790)
                     /locus_tag="IV041_000499"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8381320.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IV041_000500"
     CDS             complement(49806..50156)
                     /locus_tag="IV041_000500"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8381321.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IV041_000501"
     CDS             complement(50286..51797)
                     /locus_tag="IV041_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8381322.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IV041_000502"
     CDS             complement(52119..53471)
                     /locus_tag="IV041_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8381323.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IV041_000503"
     CDS             complement(53624..54700)
                     /locus_tag="IV041_000503"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8381324.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IV041_000504"
     CDS             54896..56641
                     /locus_tag="IV041_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8381325.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IV041_000505"
     CDS             complement(56921..57358)
                     /locus_tag="IV041_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8381326.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IV041_000506"
     CDS             complement(57342..58715)
                     /locus_tag="IV041_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381327.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IV041_000507"
     CDS             complement(59037..59525)
                     /locus_tag="IV041_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381328.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60119)
                     /locus_tag="IV041_000508"
     CDS             complement(59547..60119)
                     /locus_tag="IV041_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8381329.1"
                     /translation="MSSDHAHYWSVFFFVLILYAYHHGIFRSSTSLQAFIQQFGKYAV
                     MIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAIGFLLVRYYGV
                     AFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVAGLTKLSFREF
                     MQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13015 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgatgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaca gaccaataat gggcatgatc tgaagacatt
    60121 tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt catttattaa
    60181 aactgcctcc t
//
LOCUS       DACDCV010000008        49972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDCV010000008 DACDCV010000000
VERSION     DACDCV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.008"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            129..1349
                     /locus_tag="IV041_000509"
     CDS             129..1349
                     /locus_tag="IV041_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8381330.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1346..4066
                     /locus_tag="IV041_000510"
     CDS             1346..4066
                     /locus_tag="IV041_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8381331.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4050..4994
                     /locus_tag="IV041_000511"
     CDS             4050..4994
                     /locus_tag="IV041_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8381332.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5063..6073)
                     /locus_tag="IV041_000512"
     CDS             complement(5063..6073)
                     /locus_tag="IV041_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8381333.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6253..6597)
                     /locus_tag="IV041_000513"
     CDS             complement(6253..6597)
                     /locus_tag="IV041_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8381334.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6590..7024)
                     /locus_tag="IV041_000514"
     CDS             complement(6590..7024)
                     /locus_tag="IV041_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8381335.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7162..7899
                     /locus_tag="IV041_000515"
     CDS             7162..7899
                     /locus_tag="IV041_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381336.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7892..9103
                     /locus_tag="IV041_000516"
     CDS             7892..9103
                     /locus_tag="IV041_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381337.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9310..10089
                     /locus_tag="IV041_000517"
     CDS             9310..10089
                     /locus_tag="IV041_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8381338.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10159..10821
                     /gene="trmB"
                     /locus_tag="IV041_000518"
     CDS             10159..10821
                     /gene="trmB"
                     /locus_tag="IV041_000518"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8381339.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10919..12673)
                     /gene="ade"
                     /locus_tag="IV041_000519"
     CDS             complement(10919..12673)
                     /gene="ade"
                     /locus_tag="IV041_000519"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8381340.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12805..13734
                     /gene="cpaF"
                     /locus_tag="IV041_000520"
     CDS             12805..13734
                     /gene="cpaF"
                     /locus_tag="IV041_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8381341.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13691..14740
                     /locus_tag="IV041_000521"
     CDS             13691..14740
                     /locus_tag="IV041_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8381342.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14737..15015
                     /locus_tag="IV041_000522"
     CDS             14737..15015
                     /locus_tag="IV041_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8381343.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14990..15460
                     /locus_tag="IV041_000523"
     CDS             14990..15460
                     /locus_tag="IV041_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8381344.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15477..15779
                     /locus_tag="IV041_000524"
     CDS             15477..15779
                     /locus_tag="IV041_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381345.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15796..16167
                     /locus_tag="IV041_000525"
     CDS             15796..16167
                     /locus_tag="IV041_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8381346.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16139..16498
                     /locus_tag="IV041_000526"
     CDS             16139..16498
                     /locus_tag="IV041_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381347.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16670..17677
                     /locus_tag="IV041_000527"
     CDS             16670..17677
                     /locus_tag="IV041_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8381348.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17702..18886
                     /locus_tag="IV041_000528"
     CDS             17702..18886
                     /locus_tag="IV041_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8381349.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19323..20906)
                     /locus_tag="IV041_000529"
     CDS             complement(19323..20906)
                     /locus_tag="IV041_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8381350.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21484..21627)
                     /locus_tag="IV041_000530"
     CDS             complement(21484..21627)
                     /locus_tag="IV041_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8381351.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21849..22328)
                     /locus_tag="IV041_000531"
     CDS             complement(21849..22328)
                     /locus_tag="IV041_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8381352.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22401..22685)
                     /locus_tag="IV041_000532"
     CDS             complement(22401..22685)
                     /locus_tag="IV041_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381353.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22763..22957)
                     /gene="ssrS"
                     /locus_tag="IV041_000533"
     ncRNA           complement(22763..22957)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV041_000533"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23029..24315)
                     /locus_tag="IV041_000534"
     CDS             complement(23029..24315)
                     /locus_tag="IV041_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8381354.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24453..25124
                     /locus_tag="IV041_000535"
     CDS             24453..25124
                     /locus_tag="IV041_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8381355.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25090..25578
                     /locus_tag="IV041_000536"
     CDS             25090..25578
                     /locus_tag="IV041_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8381356.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25591..26538
                     /gene="prmA"
                     /locus_tag="IV041_000537"
     CDS             25591..26538
                     /gene="prmA"
                     /locus_tag="IV041_000537"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8381357.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26539..27303
                     /locus_tag="IV041_000538"
     CDS             26539..27303
                     /locus_tag="IV041_000538"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8381358.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27536..29749
                     /locus_tag="IV041_000539"
     CDS             27536..29749
                     /locus_tag="IV041_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8381359.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29760..30206
                     /locus_tag="IV041_000540"
     CDS             29760..30206
                     /locus_tag="IV041_000540"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8381360.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30377..31636
                     /locus_tag="IV041_000541"
     CDS             30377..31636
                     /locus_tag="IV041_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8381361.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31725..32672)
                     /locus_tag="IV041_000542"
     CDS             complement(31725..32672)
                     /locus_tag="IV041_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8381362.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33066..34358
                     /locus_tag="IV041_000543"
     CDS             33066..34358
                     /locus_tag="IV041_000543"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8381363.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34373..36142
                     /gene="aspS"
                     /locus_tag="IV041_000544"
     CDS             34373..36142
                     /gene="aspS"
                     /locus_tag="IV041_000544"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8381364.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36251..37126
                     /locus_tag="IV041_000545"
     CDS             36251..37126
                     /locus_tag="IV041_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8381365.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37392..39275
                     /locus_tag="IV041_000546"
     CDS             37392..39275
                     /locus_tag="IV041_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8381366.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39350..39634)
                     /locus_tag="IV041_000547"
     CDS             complement(39350..39634)
                     /locus_tag="IV041_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8381367.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39624..40358)
                     /locus_tag="IV041_000548"
     CDS             complement(39624..40358)
                     /locus_tag="IV041_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8381368.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40487..41110
                     /locus_tag="IV041_000549"
     CDS             40487..41110
                     /locus_tag="IV041_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8381369.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41302..42423
                     /locus_tag="IV041_000550"
     CDS             41302..42423
                     /locus_tag="IV041_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8381370.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42430..45558
                     /locus_tag="IV041_000551"
     CDS             42430..45558
                     /locus_tag="IV041_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8381371.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45577..48771
                     /locus_tag="IV041_000552"
     CDS             45577..48771
                     /locus_tag="IV041_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8381372.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48917..49624)
                     /locus_tag="IV041_000553"
     CDS             complement(48917..49624)
                     /locus_tag="IV041_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8381373.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16293 a         8790 c        10424 g        14465 t
ORIGIN      
        1 aataaactta ttttttgaag tcggccattt atcgtaattt ctttgcataa tacgaacaaa
       61 ttttcggtta ttgatgaaaa aaaacattga aaatggtatg ataaaagaaa aaaaggagga
      121 aatttttaat ggtgaaattt atccatgcgg cagatttaca tatggatcgg tcatttgaag
      181 gactgacaac tcttgataaa actgtacagg aaaaactatt gaagacgaat ctgaccgttt
      241 tatcaaatat cattgaacag gcaataataa ataacgttga ttttgtttta cttgcaggag
      301 ataattttca tcaaaatcgt ccttcattaa aaatacagaa acatttttca gaacagatga
      361 aacggttaga aaagaaccat atacctgtat ttataatatt cggaaatcat gatttctatc
      421 aaaaagagcg gtattggttc tcctttccta aaaatgtcca tttatttaca tccgaaacgg
      481 tagagacaaa aaaaataacg ataaaaagtg gagaaactgt ctctctcagt ggatttagtt
      541 atcgtcaacc gtggattcaa aaagacaaag tgatggaatt tccttctaga gaactcacag
      601 attaccatat tgggttgtac cacggggaac ctggcgtctc tcaaaaagga aactatgctc
      661 ctttccaacc ctcaaagatg caagaaaagg gatacgatta ttgggcttta gggcatatcc
      721 atgtgccaac ggtcttaaat gaaaagcaaa caattgttta tccaggtgcg cctcaaggtc
      781 atactaaaaa ggaacaagct tctacatcta ttctactagt tgaattaagt aaaggaagtt
      841 gccaaataca tccgattaaa gtagcagagg tctattggaa gacaaaggaa atatcattga
      901 ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg tcagcagctt tcaaaagtag
      961 aggatggatt tagtttaatt gagataaaat taaaagatta tgaccatctc aatacagatg
     1021 ttttggaacg gatacaatca ggagagctat tagactattt gttagaggaa ttctcagata
     1081 ggataaatga tttctttatt tggcgtattt ctctcataga gaatactttt tatacgaaga
     1141 ccccacttgc tgcttctgct aaattgatgg aacagttatt tcaatactat cagacacctc
     1201 aagatttcca acaaatttta aatgaagtat atagtcatca agaagcggct agaatattga
     1261 gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc caagcagctg cttaatcaag
     1321 attttttatt tgaggaggat caggaatgaa gattttaagg atcgagattg ctgcttttgg
     1381 aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat cagctgatct atgggggaaa
     1441 cgaagcagga aaatcaacga tttatcaatt tattcaagca atattatttg gtttcccttc
     1501 aaaaggccgt aaaaaaaagg attatacacc aaaagacggc tcagcctatg gcggcaaaat
     1561 atggcttaaa catcctgtat atggtgaatt tgctgtcgag cgctacaagc agcaaaatcg
     1621 cggaaaatcg aaggtgtggc tgggggatca agtgggaagt gatgagttac tagaaaaact
     1681 gatcgcacct ttgacgaaag agttattcca gcaggtattt actttccagc aagagcagtt
     1741 gatggaacta gatcatttga gagaaaaaga gctacatgat gcgctgattt ctttaggcat
     1801 cacgggcagt tctcaagtat tccaaaagcg gcaagaatat tatcagaaag cacaacaatt
     1861 attcaagaaa aaaggccaaa aattactaat caatcaaaaa ttaaatgaat ggaaagagct
     1921 gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat tttggcaagt tgatccaaca
     1981 agaacaggaa tacgagtcgc aacagcagca gttattggca caattaaaag aggcgaacaa
     2041 acaattttca ttagccagac agcaagaact taatttttct ttatatgaag aatggcaatc
     2101 tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa cttcttttag atctggagtc
     2161 tttctccaaa cggtatcagc aaacaaatga gcgtttggaa gaattagaag caacactgga
     2221 aaagcatagc gggatggatc agcagtcagc cagatattat ttttatttag agcaggagca
     2281 ggaactaaaa gatctccagc aacttcaatt tacagcggaa caattaaacg aagaatggca
     2341 aagattacaa attgagacta gagaaacaaa ttcagaattg aagattttgg aacaaaggtg
     2401 gcattggcag aaaaatccgt taccggaaga atttttatat gaagaagaat ggaagcatct
     2461 tctggctgaa gaaagaagga ttgctgatct agaaagccag atttctgtgc gtctccaaat
     2521 agctaaagaa cagcaagaga tgattgaagc agaagtgaat gacttggaag agacctatcc
     2581 agcattatta caaaatcagt cagagatagc accagtctct tcatctaaac cgtatatgct
     2641 gatggctggc ggagcaataa tcattgcagg tatatttgta ccaatgccct tgaaagtgat
     2701 ccttttacta attggtgcag gcatgattgg ctatttcttc tacaaaaggc aaccaaaaat
     2761 taaaactaaa aatgatttct ctgagataaa aggaatgtgg caagaaaaac taggacaact
     2821 tgatctagcc ttagctcaat tagctgaagc agaagaaaaa caaaagagaa ctggagaaga
     2881 aaaacagatt cttcagcaag aagtatatcg ttttgcggat catcggcact taggaaagat
     2941 ggatacgctc aaactcatga aagaatatgg acaggaagtc atagattatc agaaattata
     3001 tggcgtattt gagcggaaac ataaaagaga acaggaaata caagaaaaac tacgacaaat
     3061 cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata caagataaga caatttcaga
     3121 aaaaatggta ttgctttccc gttttataca agagatggaa cagatgaaat ttgccagaag
     3181 ctatcagcaa aatactttat tgaagcaaga aatcaatcaa ttgaaaaaga aacaaaaagt
     3241 atggatacaa gaatacagcg aacaattgac tcaagcaggc ttatcttatc cttcggaagt
     3301 acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag atgaaaagat acaatgaatt
     3361 atcggaaatg cttactccac tatttcctga agagctgaca aaagagaaat tatctgtaag
     3421 actgcgccaa ctgaaagaaa agcaatccca actccagcaa gaagaaaaag aagcaagtga
     3481 aaatcggcaa cgactccaac ttcaagtaga agagttgcaa agagacggca cattggacga
     3541 actttatcaa caagctagcc ggcaaaaagc aaaattacag gaattactgg aggaatgggg
     3601 agcacttgaa atcgccggga cattcttagg tgatctttca acagagatga gtgaaagaca
     3661 attaccccag ttgttaagag cagcaggtga ttatttaggt attttaacaa aaggaaatta
     3721 tcagaagata ttactgaaaa atgatacact gactttaaca gacggatata cagaatttcc
     3781 catctatgag ttatctacag gtacgaaaga tcagctgatt atggcgatga gatttgctta
     3841 cctctcatta gaagctaacc gttcgatatg cccgatcatc attgatgatg gctggcttca
     3901 ttatgatcat cagcgaaagt atcaattagc tgaactttta gctaaatttg gagaaaagta
     3961 tcaaataatt tgtttttctt ctgataaaga aatggtaagc tattaccatg agatgaacca
     4021 aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat tcgtgattta gtaatcgatg
     4081 agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt aagaatcgct aaaaacggaa
     4141 aaaaatttat tgcttttact ttccaagata cttcaggtac tatcgatggt aaatactggg
     4201 atgcttcgga ggaggaaatc aataaattta cagccggcac agtcgttttt ctaaacggta
     4261 aaagagaggt ttatcaagga aaccctcaag taaagattct tcatatgcgc ttaacaaggg
     4321 tagatgagcc aggagagcct agtttataca tggaaagagc ccctttgaag aaagaagaaa
     4381 tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa tgcgcattgg aatcgtattg
     4441 ttcgctatct acttaataaa tatcaaaaac aattttttga gtttccggca gcaaaaagaa
     4501 accatcatgc atttgccagt ggattagctt atcatacttt gacaatgctt cacttaggga
     4561 aatcaatcgc cgatgaatat cctgagctga atcgagcatt gttgtatgct ggtatcattt
     4621 tgcatgattt aggaaaggtg attgaacttt ccggtcctat gtcgacagaa tatacgcttg
     4681 caggaaattt gatcggtcat ctggtattga ttgacgaaga aataactaaa gcctgtcttg
     4741 cgttgaaaat cagcgaagct gacgaagatg ttcttgtatt acgtcatatg gttttagcac
     4801 accatggctt attagaatat ggctcacctg tacgtccaag aataatggaa gcagaaatat
     4861 tacatcaaat cgacaatatc gatgcttcga tgcagatgat gttgacttcg attcgtcaaa
     4921 cagaaccagg aaaatacacc gatcggattt tcggtatgga caaccgaagt ttctatgtac
     4981 caaaagattt atagcaaaaa tttatttttt tcattcaaaa caaaaaaagc gagacataag
     5041 tcttcgcttt ttttgttttc ttttattttg atgaatcagt tgtttcttca gttgaagatt
     5101 cttttgttga atcagttgat tttgtgtcag aactctttgt tgaagctgta gtttcacttg
     5161 agtctttcgt acttgatgaa gttgttgtga atgcactcaa cacattttca aaggcatcat
     5221 ctttgatttt tacatttgca tcttttaatt cttcaccgat tactttagtt gtgaatgcat
     5281 tgtcacttag tttagtttca gtagcgatat cttttagttg atctttgtat ttatccatgt
     5341 cattgccttt attttggttt ttcaccattt tgacaacgta gtattctgtc gcataagaag
     5401 taggatttgt tgtagtaata acatcggaga tctcaccgtc tttcaatttg aaagctgctt
     5461 cttttacttc tgcaggaata gttgttgtcg ttgagtcgaa tttaacttta ccgccatctt
     5521 cttttgtctc tgtatcagtt gatttgtctt tggctaattt agagaaatca tctccatcat
     5581 ccgctgattt tttcacatct tttgcttcat cttcacttga taatttgatg atttgtgctt
     5641 ctacttctgg atggaaagat ttccaagcag ttttcaaatc gtcatctgta atatctacgt
     5701 gagctttcaa gccagcttca aaagctaaat tgttacgaat atattcttta taagtatctt
     5761 tcgtatatcc agcagcttct aattgtgatt caaaagtatc acctaatgat ttcgcttgtt
     5821 tgtcgtattc tgcatccact tgcttatcgg ttactttatc gccatatttg ttattgaata
     5881 ctttataaat gatcatccgt tgtactaagg attggttcga tgattcaagt tttgcttcat
     5941 catagaaatc agatacagtg atcgttccgc ctttcatcgt tgcgatatct ttattagtat
     6001 cgcctgaaca agctgctaga gttaaaacag ccaacgcact agtagctgcc aaaataattg
     6061 attttttttt cattaaaatt tgcactcctg taattagtta ttatttttat atattaacta
     6121 taccatatcc catttccttt aaaaacgttg tcgtggtagt ttcaaacaaa attcaaaatt
     6181 tcatcacatt cctaggaaat acgcaaactt ttctcactta tatagttaaa tctgttgaga
     6241 aaataatcat tattatattt gttcaggata ctctggcaga tcagctttta gtttttctac
     6301 ttgttcattg atttgtgtga tacgtggttc ggcttggaat ttaaaatctt cgatatcttt
     6361 tttgattgct tcttggaaag caggaatcac agattcagct gtttcttttg tttcgatcaa
     6421 tgcgctttta aaatgattca ggctgttatt taaatcattt gtcagagttg ttgcttcttc
     6481 caattcttcg atcagtttct ttcttgtttc attcccgctc ctgggcgcca ataacagacc
     6541 tccgacagtg ccagcagcag ctccaaataa cagtcctttg aaaaagtttt taatcatcgc
     6601 aggacacctg ctttacgatt gtttcttgaa tagcttttag ttcttccgat ccataactat
     6661 cttgatgatt gacaaagtga atagaaaaat catcctcttt gctgtagcga gggactaaat
     6721 ggatatgtga atgaaaaaca gattgatagg ctagttcacg gttgttattc aagatattaa
     6781 gacctttcat ttctggaaaa gctttttcca atgcttgagc aatcttaggc acacgcgaaa
     6841 aaacctctcc tgcaagttcg ggatcatatt caaaaatatc tgctacatga cgtttaggca
     6901 caactaatgt gtgtcctttc gttgcttgag agatgtctaa aaaagcatag accttgtcgt
     6961 cttcatatat tttatagctt gggatttctt gattgatgat tttgcaaaaa atacagtttt
     7021 ccatataaaa catcctttct ataaagaata aatacttcag cttaacttta ccataagtta
     7081 aagctattgt aaggaaaaaa acaaaatacc gtcattttct tcgtggtata ataaataaaa
     7141 ataaatggcg gaggaaaaag aatgagttta attatcgatc atgtcacagg ggggtacgga
     7201 catctccctg tgttaaaaga tatcagtttc gatgtacagc ctggagagat agtagggttg
     7261 attggactga acggagcagg gaaaagtacg accatcaagc acattatcgg tctgctggaa
     7321 gcttcaaaag gaacgataga aatcaatcat ctatccttaa agaaaaatac ggaagaatat
     7381 cgcaaacaaa ttgggtatat tccagagact ccttctttat atgaagaatt gacattgaga
     7441 gaacatatcg aagtcactgc gatggcctac gatataccta tggatatagc aatgaaaaga
     7501 gcagaaaagt tgttggagac ttttcggtta tctaatcgtc ttgactggtt cccagctaat
     7561 ttttcaaagg gaatgaaaca gaaagtgatg atcttatgcg cctttttgat cgagccttct
     7621 ttatatatca tcgacgaacc atttttaggc ttggatccac tagctatcaa tgcattgctt
     7681 gaattgatgg tagaaatgaa aaaagaagga gccggtattt tgatgtctac gcatatttta
     7741 gctactgcag aaaaatattg tgatcgtttt gtggtcttac ataacggaga gatacgagca
     7801 gacggttctc ttgagatgtt gagaaaagaa tttcagctgc cggattcttc tttggacgaa
     7861 atctatattg ctttaacaaa ggagcaggca catgagtagt ttttttcaaa aacgtttagc
     7921 aagacatcaa aagaaaatga tgaaatatat gcgttatgta ttgaacgatc attttgtttt
     7981 agtttgtctt ttcttgattg gcggtgtggg attatattat tctaattggt tgaaaaccct
     8041 gcagccaccg tttgcaattg gagggctcat aatctctgta ttttggatgc tctgtttatt
     8101 cgtgggtaag atggctactt ttgcagaggc agcagatatt gtttttttac ttcctaaaga
     8161 aaaagaaatg caaacctatt tggaacgagc ttttcgctat tcttgcattt ttccgttgat
     8221 ctttttgata ttggcatgcg gattggccat gccattagtg gtggttagta cggagcaatc
     8281 cttttcgatg tttttcgtct atctgatcat attatggagt ttgaaattca gtcatctgca
     8341 aatcaagaga gcagaattgt ttcgactaga tcaacaagtt attcgttatt ggaaattagg
     8401 ctggtttttt tcttctctga tctttttgtt tctatcattt tattggctaa tatgggcagg
     8461 aatagcaggt gcattagtac aggcaagtct atattactgg tttttgtgga agcaacaaga
     8521 aaaaggattg gattgggaga aaatggtgca tgtagaagaa cataggttgc atcagatcta
     8581 tcaatttatc aatttattca cagatgttcc tgaaattacg gcaaagatca aaagaagacg
     8641 ctatttggat cctatattga ctcatattaa aagagaatca aaaaacactt atctttactt
     8701 gtttgcaaga agattggcaa gaggttctga ttttagtggt ctttatttcc gattagtaat
     8761 tataggtggg ttattgattg ccggagtagt cgacttttat tttagtctga ttatcggttt
     8821 cttattttta tatttgatcg gtttccaact gttaccttta gcgaaccagt tccgctatat
     8881 ggttctcgtt cagctttatc caatccataa gaatcagaag aagcaggcga ttcagtatct
     8941 actcacatgg gtattaatta tcgcgtcggt tatttttggt ttgattgctg cagtcgtatt
     9001 agatgggcag cagcgatggg ttccgctagc ggcttatttg attgttacta tgctctttgt
     9061 taaactatat gtaccaactc gtttgaagaa gatgtcagac tgaaaacaaa gctgtgtagg
     9121 gtaaaaatga gaatgaaaaa tagagcgttg acagtaagaa tgctgttcgt tctatttttt
     9181 ttgagaaaag ctggtttttt gaatggcaat taaatgaaaa agaacgtata tgtgcttgac
     9241 gtgtagtgtt caggcgacta gaataataag tactgcaaaa caaagagtaa gaggacggga
     9301 gaatgcatga tggagtttca gttagaccaa gagtggcggt tacgtcccat caaaggcgac
     9361 acaggaaaaa catacatagg catgcgtgat gaggacaaag tctttattaa acgaaacacg
     9421 actccgatgt tggctgctct ttcaagggaa ggaattacac caaaacttgt ttggacaaaa
     9481 cgaacgggga atggcgatac tttgacagca caagagtggc tggaaggtcg cttgctgact
     9541 cctaatgaga ttggtcaaag aaacgacgtg attgatgttt tgtaccattt acatcattca
     9601 aattctctta aaaccatgct gacccgtatc ggtggtcgtg tcatgacgcc ggagaagatg
     9661 ctgcaaaaat atgaaaaaga attaccggaa gaactccaga aaaatcaatt tctgcaaatt
     9721 gtccatcgtt ttcttcgtga gcagatcccg tcaatagata aggaaaactt atcagtagta
     9781 cacggagatg taaaccatcg taattggtta gtatgtaaaa attatttata tctagtcgat
     9841 tgggattcaa tcatgtttgc agaccctgcg gtggatattg caacgatttt aggaaattat
     9901 gtgccactgt ctagctggaa tcagtggctg atcagttatg gcatacgccc gactgacgaa
     9961 atgcttgaaa aatttcattg gtatgcggcc atgaatttgc tccaagaaat caccagatat
    10021 tgcaaacgtg aagatcatcg acggatgaac gaagaaattt tacaattaaa acgaattttc
    10081 agtggctgag gtttggccca aactctgcgg agatttggcc agaccttttt aattagagga
    10141 aaggaatgga gataaataat gcgtgtccga aatcgtccgg gagcagcgga aatgttagcg
    10201 gctcacccga attttgtaat tagtgatcca acattgtgga agggaaaatg gaatgaattg
    10261 tttgaaaacg atcatccaat ccacattgag attggtatgg ggaaaggcca gttcatcact
    10321 ggaatggcaa aagcccatcc tgaaatcaat tatattggag tagaaatgca ggtcagtgtt
    10381 gtttcgattg cactagacaa gttgatcgaa caaccgcttc ctaacctgaa acttctacat
    10441 gtggatggat cagcgttgac agagtatttc gctgacagcg aagtagatca aatctatctg
    10501 aatttttccg atccatggcc aaaaaaacgt catgaaaaac ggcgattgac atataagact
    10561 tttcttgctg tagatgaaca gattcttcgt ccaaatggag aaatccattt caaaacagat
    10621 aatcaaggat tgtttgagta ttctctggca agtttttctc aatatggcat gatcctcaaa
    10681 caggtatggt tagatttaca tcaaagtcaa tttgaaggca atattatgac ggagtatgaa
    10741 gaaaaatttt cttcaaaagg tcaacgtatt tatcgagtag aagctcaatt tcaagataag
    10801 accaaaaaga aaagtctgtg aaactaactt tctgctttca taaaaacatt aatatccata
    10861 aataaatctc agaaaatgaa cattacagat gtaatgtatt ttctgggatt tatttgattt
    10921 aaaatgtatt tatttcatcg gtttttccac aaggtggaat caattggaag cgagaaaatg
    10981 aaaataatcc tttgtcggtg atcttcaatt cgggtatgac agataatgtt aagaaagaaa
    11041 gtgtcaaaaa aggatcaaag gttttagctg ctcctagttg atgtgcttta gttattaatt
    11101 cattcaactg ttcatagacg atcgaaaaag gttcctgtgt catgattccg cctataggaa
    11161 gagaaacaca agctatttcc tgctgtttgt tgacaacggt catcccgcca cctttttgaa
    11221 tcaaaagatt agcagcatac agcatatcct cgtcgtttgt cccaacgaca actagattat
    11281 gcgagtcatg ggcaatcgtt gtcgctatcg ctccttgttt cagctgaaat cctttcacta
    11341 tgcctaatcc aatgtttccc gtttgatggt gacgctcgat caccgcaatc tttaattggt
    11401 ctttttcgat tgaaggaatg aatcgttcgc cttgtcggtc cactttttct accaaatctt
    11461 tagtcactag actattcggg ataatttcga tcacatgggc ttgtgaagaa gaaagaagca
    11521 gctctaacga atgtggattt aatggttgag cgtttagttt tggcagacaa cacgtatatg
    11581 aatttgaatg atcagcaaat aaatggtctt ggagttgccc agattgaaca actaatgttc
    11641 ccttatagaa aacttcatga attggcagct tggtaagatc gtctgttaag aagaaatcag
    11701 cttgataccc aggggcaatc gcacctagtg ttttcaaatc aaaacattct gctgcattca
    11761 atgttgccat ctgaatggct tgtaatggat cgattccaag tgcaatagct tgacggacga
    11821 tatgatcgat tgatccttct tttactaaat cattgatcag tttatcatcg gtgacaaaga
    11881 agcatcttct tgaattttca ggtgtgatcg ccggtagtag cgatgccaga tcttttgcaa
    11941 cggtcccttc tcgaaccatt aaatacatac caagatccaa acgttcttta gcttcttttg
    12001 cagttgtagc ttcatgatcc gtccgaatac ctgctgcagg ataaacattt aactcttcca
    12061 tgccgatacc agctgcatgc ccgtcaattt ttttcttatg ctggtgcatc aattggatct
    12121 tttctaggat gtctgtttca ttatttgcta ctgcttgata gttcatgact tcagctaagc
    12181 ctaaaacttc gggtttttgt aaaaaagggg ctaatttttt tgcatttaaa tttgcaccat
    12241 tggtctcaaa aggggttaca ggtacacaag aaggtagcat aaaaaagata ttcatgagtg
    12301 tttgtcttgc atcttctatc ataaactcaa gacctgtact cccagcaatg tttcctattt
    12361 catggggatc tgtcactaca gtagtaactc catgaagcaa ggatactttt gaaaattcgc
    12421 ttggtgataa aagtgagctt tcgatatgca catggccatc gatgaatcct ggcactagat
    12481 attgcccatt agcattatgt acgctttttc cttcgtattc accgattcct acgatcattc
    12541 cttcgcagat tgctacatct ttttccatga tttctttcgt aaagacgttg acgatcttag
    12601 catgcttgat cactaggtca gcaggctttt ttttacttgc tacttcgata tatttcctta
    12661 atgttgtttc caatggagtg cctcccaaga taaattttta taagtataac ataaattcgg
    12721 cttttgccaa tatttttttg taaattcatt ttatttgaaa caaaaagatc aaaaagacgt
    12781 atgttttttg tggaggggag aataatggaa ataaaacgat tatcggatca aatgataagt
    12841 gaaagttttg ccaaacatgc ccaagacgta tatatattgc cagcttcaga agtattttac
    12901 atctattttc gctatggaaa aaaacgaaca ttctggaaga aagtctcttc ggcagatgga
    12961 cagcaactga tctcaagatt taagtatttg ggacagatgg atatcggaga aaaaagaaaa
    13021 gctcagttag gtgcatttac ttattcttta gaaaaacagg aagttcgtct gcggttgtct
    13081 tctgtaggag attaccttca acgagagagt ttagtgattc ggctattata cggtactttt
    13141 gaaacacccc atcgttgctt tagttctcga gattttgcaa ctattttaca gaaaaccgtc
    13201 agacgtggat tatacctttt cagcgggaca gtcggatcag gcaagacttc tctaatgtat
    13261 cggttggctt tggaagaaaa attgcaagtg attactatcg aagatcctgt agagatagaa
    13321 gaaccgtttt ttctgcagct acagatcaat ccgaaaatag cacagactta tgatacactt
    13381 ttgaaactcg cattacggca tcgtccagac ttgctaatca ttggcgagat aagagatgaa
    13441 tcaactgcac atgctgcggt aagagcagca ctaacagggc accgggtttt tgcaaccgta
    13501 catgcacgca atcttgaagg tacattggtc agaatccatg aactgacaaa gaaaaaagaa
    13561 gagctatctg aatgtttagc tggtgtcatt tatcaagaac ttttggaaga tgccaatcac
    13621 catccttctg ttttatggag ttatcaattt atggaggaag aatcagtaaa aaaatcatgg
    13681 gaggaaagtt atgaagaagc tcaggaaaat atttggcagt cagaactatt ctaaaggatt
    13741 aacaagaagc cagcaaaacc agtttgcaca aaccatggga gaactgcttt tatctggttt
    13801 tagtctgcaa gaatcccttt atttattact cacgatccgt tcgtttccac ctgttatttt
    13861 ggcaaatatc caacatcagc taaaggaagg gcaagcgttt agtgaggtac tttcgcaaat
    13921 aggatatgga gcagagcaac tggtacagat cgagttagct gaacgtcatg ggaatctgcc
    13981 gacaactttg ttggaaattg ccaaacagtt tcggcttgtt gaaggatttc gcaatgattt
    14041 gaaaaagctg cttgcatatc cgatgctatt gctcaccttt ttgctaggta tcttgtttag
    14101 tatgcggttg ttcgttttgc ctcagcttat tggttcagga atggtcatgc aagagcattg
    14161 gggtattcag ctgatacaag tctttcattg gtatgttcta ctattcatcc tagcggttac
    14221 tctattggct ttactagtaa ggtatcgtct gatgaagttg tctcctgttg atcagagtga
    14281 atggataagc agacaattat tgattggacc gatatattcc ttgtatcaat cggcttattt
    14341 gtcactagag attggaaagc tttttcatga aggtttcgaa ttaaggcaaa tcatagtctg
    14401 cttaaaggaa acgaaacaag aaagtctgat tcagcatctt gcctctaagt tgattcaagg
    14461 attggaggca ggtatgtcat tggctgagca gttccagaat tatcgttttc tatcagaaga
    14521 attcagtaaa attgttttac agggagaggc aaagggaagt ttaggtaaag aactgatttt
    14581 ttacagcgaa tggacaagaa aacagctttt caaacgtctt catcactgga tgcattttat
    14641 tcagccaatc atttttttgg gcgtagcggc tttgatttta ttagtttatg ccgctgtttt
    14701 attacctgtt tacggaagca tagaggaggt tttgccatga aaaagagaaa aatatcttat
    14761 tatagcggtt ttacattgat cgaaatgcta atcgtcttac tgatcatcag tgtattagtg
    14821 ttgttgttcg tccctaattt gtcgcgttat aggaatcatg tagaccaaga aagtcgtgaa
    14881 gcgatcatcc agttggtaga cacacaaaaa gagctctatg cgcttcaaaa taatggaaga
    14941 gttcctactg tggaggaatt gttgaatgaa ggctatatca aacgagaaca tgcagaaatc
    15001 tatcagcggc cttgaaggta aggttttagc atcaggatat acgttgtttg aatcactctt
    15061 actgatttta ttacttacaa cgatcctttc ttttccagtt ctcgcatttt ctgcatggaa
    15121 gaatgaattg gcagtctatc aatttttcgg ccaatttgaa aaacgaatct attctacgca
    15181 aaaaatcgct attgtgaatc agatccaaac aggcttttat tggaatagtg aagagaatca
    15241 aatcattttt catgttccga atccagataa aataaattgg gaagtattag atattcctga
    15301 tgagattacc ttgaaacgcc atgcttctat tacttttgct gctagaaccg ggaatgaaag
    15361 ttcactaaaa gcttatcaat tttattggga agaaaaagag cggacgatta cgtatcaatt
    15421 ccagataggg agtggtcgct atacaaaaag aattgaataa aacaggattt gttttgttgg
    15481 agtcccttac agcgcttatg atcgcttctg tctgtgtcta cttagtgagc tttggccagc
    15541 tgtttttgct tcaggcagaa aagaaaagtg gggaggaact gctatttatt cgaatgattt
    15601 atgaagatat caagacgcag cggatgtatc acaaaagacc agagcgaagg gaagaaatga
    15661 tcccatacga tatccaaatt gtcgataata atggagtaat cgaaaaagtg tttgtcaata
    15721 tggagaaaga agcgatcagt attgaaaaaa caagtaattc cagcatttac gctgattgaa
    15781 tgtctaattg gactgatgat attgagcagt ctattgctca cattcaatct tttgattcag
    15841 caaaccatcc aagtaagtga atttttgcag cgtaaagatc aaaaagagtg gctaatattt
    15901 ttagcacaat tagaagaaga actcaagagc agtcacaatg tctctgtgaa aaatcagcaa
    15961 ctgcactaca cgatcgagga taaacagtat atcatcgaaa gatatcaggc aatgatacgt
    16021 aaaagaagaa cttccggcgg acatcaacct atgttaacaa gtatcactga gcttcaatta
    16081 ttggaaaaag aacacacaat ttctttttat gtacactttg aaaatggaga agatggatat
    16141 gccatatgga caaaaaacga tcagtagaaa atgttataag ggctctattt tattttctgt
    16201 cttgctttta tttcttttat gtacgttttt attgatggca gcattagaaa gctaccgttt
    16261 gtcaatggat cttaatatac gaatcaaaaa ttattatata gcaaaaataa tgaagcagat
    16321 ggctttatcg gaacagtcta cgcttgccga aagttcggaa ggagtttttt actacacaac
    16381 tggatctgta acatatcagt gggtacaaca gtccctattc ttagaggtgg aagtctcacc
    16441 gtttatcttt cggttcacag aagaagtaaa aaatgataca gatactagta cagaatgatg
    16501 gaatgttcgt gaaaacgccc tcttttctgt tataacactg tttttaataa aaaaatgttt
    16561 gccgtttcac aaataaaaaa cagtgaaaac gattgaaatt ttgtcaattg ttcgatagaa
    16621 ttaaacaggt gaaaaaattg ttataatata tctagaaatg aggtgcacca tgttaccaga
    16681 aaaaatggaa caagggttta atcaaaacct ggaagctatt cagcttttac agaatgcatt
    16741 gggaacttcc tttttagaag cctatgtcga aaatgcagaa aatttaatag atgattatca
    16801 agtacgtgtg gtggatggag tgccgacaaa agagacaacg caacggatca cagctcttta
    16861 tgaagagttg aagaaacttt ctttcgagcc agaagaatgg cgcagactgt ctcaattact
    16921 tttattgaag ggcagccaga cagaacattt gcaggcgaac catcaactca ctcctgatag
    16981 tatcggtttt ttatttgttt ttcttattga acaactatac acaaataaaa aagcaccggt
    17041 taagattctg gatattgcgg caggaatggg caacttgtta ttaacagttc ttttaaacct
    17101 atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat attgatgata cattgttggc
    17161 tgtcgcagct tctacaagtg atttgacaca agcaaatgtt caatactttc atcaagacgg
    17221 gttgcaggaa ttattaatag atcctgtcga ttttgcaatc agcgatttgc caatcggtta
    17281 ttatccgaat gacgaaaaag caaaagagtt tttaacaagt accgaagaag gccatagtta
    17341 tgcacatcat ttattgctag agcaatcaat gaaatatgtg aagccggatg gttttggttt
    17401 gtttttgatg ccgagtggat ttttagaaac ggatcaaagt gaagaaatca aaaaatggtt
    17461 taaagaagaa ggttatcttc aaggaatgat tcaattgcca gatgaattgt tccgtaataa
    17521 acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga ccacaagcac aacaagtaaa
    17581 agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga cggaattttt
    17641 caacgagttt aaaaactgga aatcatctgc tctgtaatca ttcaacgaga aggagagtca
    17701 tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc aattatatca
    17761 aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt tacaagattc
    17821 tatttttacc atcaagtacg gcgataatca aaaattcgaa caaatcatgg atatcgaaaa
    17881 ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa ttttaagctc
    17941 ttatgatgaa atcaccggag tagggcaccg tgttgttcac ggaggagaaa cttatgggga
    18001 ttcagtagtg atcgatgaga aagtaatgga ccgcattgct gaattagcag aatttgctcc
    18061 tttgcataat cctgcaaact taatggggat caaagcgttt aaaaagattt tacctgacat
    18121 tttgagtgtg gctgtattcg atacatcttt ccactcaaca atgccaaagc aaaattattt
    18181 atatagctta ccggtgtctt attatgaaga tttcaaagta agaaaatatg gcttccatgg
    18241 cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac cgatcgaaga
    18301 actaaaaatc atcacttgcc acttaggaaa tggtgtatcg atcacagcag tcgatggagg
    18361 taaatccatg gatacttcta tgggattcac accattagct ggggtgacaa tgggtacacg
    18421 ttcaggtgat atcgatccag ctgttctccc atatttaatg gaaaaattag agattgatat
    18481 tgatgaaatg atcaatgttt tgaacaagaa atctggtctg ctaggcctaa caggattgtc
    18541 tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt tagcttatga
    18601 cattttcgtg gaccgcatcc gaaaatacat cggcggttat gtgacaacga tgaatggtgt
    18661 tgatgcaatt gtctttactg ctggtatcgg cgagaatgac ggacatgtac gtagtgaaat
    18721 catcaaaggt atgtcttggt ttggttgtga aatcgatcca gcgttgaatg aattgcgtgg
    18781 tgaagaagta gatatctcta caaaagaatc aaaagtaaga gtattagtga tcccaacaga
    18841 tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca aagcattcgt
    18901 taggataaaa gatgaaacgc atgcaagagg gaattcttgc atgtgttttt tttgcggaga
    18961 agaccgcaaa atggtaattt aacttcaatt tcccttcttt tttacaggat cttcttaggg
    19021 aattttgctt ggaaaagtgg tcaaaaggga aaagactggc attttattta ttgattttca
    19081 ggaaaatccc aatattttat tttcattcat ctttataagg gacattacat atgtaaaaag
    19141 aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct ttatgtttag
    19201 ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt ttccacctgt
    19261 actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg cgaaaaaacg
    19321 tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc aataacgagt
    19381 gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag gagcgttcct
    19441 tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag gtgattggtt
    19501 ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa cgccaaacgg
    19561 attttttcac caccggataa atctttgatc cgtttaccca catcgctttg ataaaagcca
    19621 cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat ggcgtaagcc
    19681 agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc gatttgcaag
    19741 ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa taatttgagt
    19801 aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc acgatagact
    19861 gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa gttttgaatg
    19921 atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg atttttcggg
    19981 ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc tttggctttt
    20041 ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg aatcatgact
    20101 ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc agccaagcga
    20161 gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa tgcaccttct
    20221 tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg agaaacgacc
    20281 agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact gttaagatct
    20341 aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa aattttggct
    20401 aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc ctgacagtgt
    20461 ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta accgcctagt
    20521 tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa gtttgtatct
    20581 ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg tatctcagcg
    20641 gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc ttgttcgacc
    20701 gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc ttctctgccg
    20761 gtcaagattt gtaaaaaggt tgatttgcca gttccgttga tatcgactaa ggcaattttt
    20821 tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc gattgttttt
    20881 ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca aaaaggccat
    20941 ggttatccct ggcctgtgtt atttaaactc agattatcat gagaaaaata ccttgagatg
    21001 gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca acagcaactg
    21061 taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca ttacttgtta
    21121 ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt tccagtgtac
    21181 ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat cgatgtgtaa
    21241 ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc acatcattgt
    21301 attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa aactttgtct
    21361 acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct tctctttgga
    21421 aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag cggtagcctt
    21481 tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt acccgaaccc
    21541 gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt catatttttt
    21601 cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg catgcttagg
    21661 tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt tattttactt
    21721 gaactcattg actgttttcc ctttatttta aacattataa agaccgatga gtgcttgccg
    21781 cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg
    21841 tctacgtgtt aagctttggt gtggtcttta gctggcaatt ggttttctaa atcagttcgg
    21901 acaacaagca catcgcaagc tgcgtttcta ataacatatt cagaaacaga accaataaat
    21961 aaacgttcta cagcattcaa tcctgtggct cctaacataa tcaaatctac ttgattatct
    22021 tctgggattt ctctggcaat aatttgtttt ggtgagccat attctacgac agaggttaca
    22081 ttattcaagc cagctttctt cgcattgctt tcataatctg ccaatgtttg ctttgccatt
    22141 tcagtagctt gttctgcaag cattccatca aaggatgaaa cggtttggaa agaacgtgtg
    22201 tcaatgacat gtgctaatag aagttctccg ttatttcgga tagctacatt gaccgccttt
    22261 ttaaaagcta attcagcttc atcagaacca tctacggcaa ccatgatttt tttgtattgt
    22321 tgtaacataa gcattcactc ctcaagtcaa tcgtattgta ctcttttatt gtagaacact
    22381 ttagggaaaa agagaattat ttactgcttt ggttcagcaa ttttttttgc aaatcggata
    22441 ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga ccaaggcaat aaagcgttga
    22501 ttgaaaaaag ctgttaaaat acccattacg cctaaaacag catatgcagc accgtaaaat
    22561 tgtaggaatc cttgattttt atcatttttg ttgatcaata caaaaaagat atcctgttta
    22621 ttaaataaat aatagctaac gaataataat actaaagtgg cgataatcat taaaatccta
    22681 atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc caagagcaaa
    22741 ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt
    22801 gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac caaatgataa
    22861 ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa tgttcggtca
    22921 acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta gcatatgaat
    22981 ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct agttttttgg
    23041 aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct gttcatattt
    23101 ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta aatattgctg
    23161 attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac ggttgagtga
    23221 tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag ctttgccctc
    23281 tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt ttggcgacaa
    23341 gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc
    23401 agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta aacctatatc
    23461 ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg cctcaactaa
    23521 acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt tttgaaaagc
    23581 cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt ttctttggac
    23641 acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg aaggagtcga
    23701 tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga
    23761 aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt
    23821 atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg gtttgacttc
    23881 aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat tctcagtagt
    23941 cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat cctgttttgt
    24001 tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca tttttgcttc
    24061 ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca tgcgaaatgc
    24121 ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac caggaggtcc
    24181 atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga tttttccttc
    24241 cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca tgcgataagc
    24301 caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa ttatatcata
    24361 tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag cttatccaac
    24421 taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag atagatgaat
    24481 ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg tatttggagc
    24541 atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca tacctaggtc
    24601 cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg cgggcaatgt
    24661 atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt ttgaacatca
    24721 ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa
    24781 tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc agcaacggtc
    24841 cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag tctatagcag
    24901 atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc
    24961 ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt
    25021 cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat tggggttgga
    25081 ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat tgaaaaacaa
    25141 gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat cgagatcgtt
    25201 gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga agggatcgtt
    25261 tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc tcagaaatct
    25321 gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa agggttggca
    25381 aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca agggcaaagc
    25441 gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct tcactgggat
    25501 aatcaacaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga tacttattta
    25561 ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa agtagaaact
    25621 gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc gagtggtgtc
    25681 gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg tgagatttta
    25741 gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta ttttccggaa
    25801 acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca attacctgag
    25861 tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga aagcaactgg
    25921 gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg
    25981 ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac actagatcct
    26041 ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac agcactagaa
    26101 acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt
    26161 attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc
    26221 gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt ccatgtatca
    26281 gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc gaacatttta
    26341 gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca aacaggcacg
    26401 ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc catgttagaa
    26461 caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat tattttgaaa
    26521 aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga atcaaaagac
    26581 tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag gatggaaccc
    26641 gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga aatcacggag
    26701 attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca aaaggaactg
    26761 ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga ttggatcgtt
    26821 cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc agcctctgtc
    26881 gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa
    26941 gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt ggaaacgaaa
    27001 caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta tgaggaatca
    27061 gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa accagatagt
    27121 cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt cgattatttt
    27181 gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac agaaacagca
    27241 cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc ttctttcagc
    27301 taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata gtcaacaatt
    27361 tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa aaagctttta
    27421 aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac aattatcaaa
    27481 cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac
    27541 aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat acatgggtcc
    27601 cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac atgacggaca
    27661 gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag gtatattagc
    27721 agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg ttgttgaaga
    27781 tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg ctatgttagt
    27841 ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc agctagcaga
    27901 aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca tggtcaaatt
    27961 agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata agcaaagaag
    28021 gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag ggatcagccg
    28081 gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac agtattaccg
    28141 tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt
    28201 tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct atggacggcc
    28261 aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat
    28321 ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt atgctgtatt
    28381 aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt atattgccat
    28441 gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg caggaaatcc
    28501 ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg gggttgctgc
    28561 acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca
    28621 attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg cttctgaatt
    28681 tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca caccaaaagg
    28741 agacgttact gagttaccaa aaggatccgg accattggac tttgcataca gtatccatac
    28801 agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta
    28861 caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat ttggcccaag
    28921 tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca aacgtttctt
    28981 caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat
    29041 tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc aagaagcact
    29101 cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt atggagaagt
    29161 tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga
    29221 gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat
    29281 gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac taattcgtat
    29341 cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca ctaaaggcag
    29401 agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac caaatgtagc
    29461 agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag agtatgatgc
    29521 agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac ttcaaacagt
    29581 caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag ataaaatggc
    29641 gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta tcgtggataa
    29701 aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg aggacagata
    29761 tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg
    29821 gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat acgcaaaaag
    29881 atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa
    29941 aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct caattcacac
    30001 tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca cgaccagaaa
    30061 cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc atcacagtcg
    30121 ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga cctgtaacga
    30181 tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa aactatgaaa
    30241 cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt ctgcaaaaag
    30301 cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag ttatttttta
    30361 taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca cagatttaaa
    30421 aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag cggacgctcc
    30481 cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat catcagcagc
    30541 aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa aattagtatt
    30601 gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc atcaccatcg
    30661 gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc atcgtatctt
    30721 agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac tatttgattt
    30781 aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt tatcagcgat
    30841 ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca gcggaacgtt
    30901 tacttctccg attgatcaca taaaaggtgt cgagaatcgc aaaaaatcag aaaaatgggc
    30961 gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt
    31021 atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg aagtggcagc
    31081 tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg cggcattgat
    31141 gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg acttttcgac
    31201 gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc caatgctacc
    31261 tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag gcagttgggt
    31321 tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc cagattcctt
    31381 tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag aatatgagat
    31441 gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag aatggatcac
    31501 tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt tctctgtacc
    31561 tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata gccgagcgct
    31621 actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca aacagtgctg
    31681 atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat
    31741 gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc ttggacattg
    31801 tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg tggattcttc
    31861 atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc aatggggcga
    31921 agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc tggatttctt
    31981 tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt tcctgtcagc
    32041 aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc aagataaggg
    32101 aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac tacatcgaca
    32161 tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac accgactgct
    32221 cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg
    32281 ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag acgttttagt
    32341 gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg ccctttattt
    32401 ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc tttttccttc
    32461 agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc
    32521 aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc atcttccaaa
    32581 ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg ttctgcgtag
    32641 acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt gtctagtcta
    32701 aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag tcagatatct
    32761 cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca atgaaagagc
    32821 gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga tctaaagttt
    32881 cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt
    32941 attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg aatttcattc
    33001 acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga aagggagaga
    33061 aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa
    33121 aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag tataatgaaa
    33181 tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg gatacaacgg
    33241 atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat gtgacgcttc
    33301 gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg ttcggcccag
    33361 agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac gaacgccctc
    33421 aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga tcagaaaacc
    33481 ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag ttggggatca
    33541 cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa gcctatcgtc
    33601 aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac tctaaacgcc
    33661 gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat caaaaatttg
    33721 tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa cattttgata
    33781 ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt aatatggttc
    33841 gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca ccaaaaatgg
    33901 gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa gaactaggtg
    33961 gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc ttgactatgg
    34021 aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg gtcgcactcg
    34081 gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac tttggttttt
    34141 cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca gcagataaag
    34201 cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa acgattaatg
    34261 tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata tatgaacatt
    34321 ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat aaatggcaca
    34381 aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag ttacattaaa
    34441 aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc tacgtgatcg
    34501 tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc
    34561 ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa gacgcggaga
    34621 aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt
    34681 gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag caatcagtga
    34741 cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat
    34801 caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata atgactttct
    34861 cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag actacctagt
    34921 tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc aattgtttaa
    34981 acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt gtttccgcga
    35041 tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg aaacaacgtt
    35101 cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg taatgaaaga
    35161 agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg aagcaatggc
    35221 tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg atctaagcga
    35281 tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt
    35341 gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca tggatcagtt
    35401 aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag tagaagaaga
    35461 cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa tcattaaagc
    35521 aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg aaatcgtcgc
    35581 tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc
    35641 gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag aagaagaagg
    35701 tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata tcccgttatt
    35761 ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga acggttatga
    35821 attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag tactagaaac
    35881 tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa cagctttaga
    35941 ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg caatgttatt
    36001 agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga
    36061 tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat tgaatattga
    36121 tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc
    36181 ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac atataaagcg
    36241 gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca cgaccaagct
    36301 ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct tttatcagcc
    36361 aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga ctttgtcaaa
    36421 atcactgata ggattcgaag taccggtatc aacaactttg tatttactga atataccttt
    36481 gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt ttatcacagt
    36541 cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta ccgatccaat
    36601 catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag cgttgagaaa
    36661 cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa
    36721 gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg ccggaattct
    36781 atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac
    36841 tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga aacatccaga
    36901 aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca tcaatgaagc
    36961 agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta cacgatacga
    37021 actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg taagtattgc
    37081 agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg agtttgacaa
    37141 aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt ttctaccata
    37201 gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag
    37261 catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa aggacacgat
    37321 tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt tatgaaaagt
    37381 tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc actgggtttg
    37441 atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg ccaactagta
    37501 tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat ttgatcacgg
    37561 atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca
    37621 gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct tcggcttata
    37681 tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc agcagcttac
    37741 tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat cgatttgcta
    37801 atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac tatttggttt
    37861 ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg atcttttata
    37921 ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat acaccaggag
    37981 gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata tggttgggaa
    38041 gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga
    38101 aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt tgcttcttct
    38161 ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg agtctgttat
    38221 gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga tggctgacaa
    38281 atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat cagtttccag
    38341 ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg ttgcatactt
    38401 tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat gtggagcgtc
    38461 cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac
    38521 cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta gcaattgcac
    38581 tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg
    38641 caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca gaaaacacca
    38701 ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca caagggaata
    38761 aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat gctgcaacgg
    38821 atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga caattatatg
    38881 ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac actgttctga
    38941 ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg atggatgcaa
    39001 tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg
    39061 acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg ctgattgatt
    39121 ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc catccaaatc
    39181 ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga ggaagttcaa
    39241 gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact tcaggcaaaa
    39301 acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt catatagatt
    39361 caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt cgggtgagtt
    39421 ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact tctgcatgga
    39481 tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta ccattgttat
    39541 gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc tgatctttac
    39601 agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat ctctgcagtg
    39661 tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc gtctaatttg
    39721 ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt tgggtaaaca
    39781 aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat ttctaagaac
    39841 cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt acttgaaata
    39901 tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag gtgtttatta
    39961 gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac gactaagtca
    40021 caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg gacagtcatt
    40081 tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag acgaggttga
    40141 agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca
    40201 tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa gtgagcaagc
    40261 agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt gatatcttct
    40321 gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa
    40381 aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt gtcaaacctc
    40441 tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt tttatcgatt
    40501 tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc gctatgagaa
    40561 taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc gtacttggtg
    40621 ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta tggcaaaaga
    40681 agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg cttttccagt
    40741 taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt tgaaatcgac
    40801 aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac ttaaaggcgg
    40861 aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc
    40921 gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat
    40981 cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg aacgacgaac
    41041 acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat atgaagtcaa
    41101 aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg tcctgttcag
    41161 ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt tttgcgttcc
    41221 aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa taattagaag
    41281 gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca tatcggaaaa
    41341 aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc tatactagat
    41401 atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata tgataggagt
    41461 gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg gaatttaaag
    41521 gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag actttcagcg
    41581 ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc agaagcattt
    41641 gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga accaattgca
    41701 gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt agccaaggtg
    41761 atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat tagccatttt
    41821 tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg cgggttggat
    41881 tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca tttgcataat
    41941 caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa attctcttta
    42001 tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg cctggagttg
    42061 aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag tttcaaagaa
    42121 ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta tatctatctc
    42181 actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata tcctcgtatt
    42241 ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta
    42301 gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac ggaagagcag
    42361 ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat ggaaatggaa
    42421 tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt tatcaatgag
    42481 accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg gaagacagga
    42541 gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga aacttccgga
    42601 agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga agaaacaagc
    42661 gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa accagaacaa
    42721 gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt aacgttgacc
    42781 atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga agcagatcag
    42841 ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc
    42901 caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac tgttttacgt
    42961 catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa
    43021 caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca aaaaaggttt
    43081 gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc ccaatggaca
    43141 aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct aacggaactt
    43201 caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt
    43261 tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca aaaagacgaa
    43321 atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt agtcgaacca
    43381 atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag
    43441 caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa aaatgaagca
    43501 aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag
    43561 atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga acaattaaca
    43621 atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct cgctatctat
    43681 caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact gcaagaagat
    43741 cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa
    43801 aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa agcaactgaa
    43861 gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga acagctgaaa
    43921 agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc aggggagcca
    43981 tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga acagccaaat
    44041 aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga
    44101 actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca acaattatca
    44161 gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga agaacacttg
    44221 caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa aacaactctt
    44281 gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatga aagaatagag
    44341 ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt ctacaaacag
    44401 cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa aagtttagaa
    44461 caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg acttgaaaaa
    44521 cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt gcaattgttg
    44581 caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat agaaaaaata
    44641 gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag ccagtcgtat
    44701 ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata taaagaaaac
    44761 caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg gctgcgtcaa
    44821 ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag ccaacagtta
    44881 gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt acaagaacaa
    44941 aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac aagccagacg
    45001 aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg agaaaatcct
    45061 aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt gttacaagta
    45121 gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct agcggatacc
    45181 attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga tcaggcaggt
    45241 atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc actttcttta
    45301 gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat cgatgcattg
    45361 tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc aatggaagct
    45421 ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt aagagaattg
    45481 aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca aagcaaaata
    45541 cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat tccagaaaaa
    45601 gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt tgtccctgac
    45661 caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat ggttgatttg
    45721 gatggtacag caaaagagat agattactgc caatgtccct attgggagga acataaatat
    45781 tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc tcgtatcatg
    45841 aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga aatcattgca
    45901 aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc tttgattatt
    45961 gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc tattttggca
    46021 ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt aaaaaacatc
    46081 tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa acagtaccag
    46141 tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca
    46201 ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa aggaaaacta
    46261 gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga gcagatgaat
    46321 cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat acaattttct
    46381 gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta tttgctgcaa
    46441 gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat ccaagggaca
    46501 ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca attgattcgc
    46561 cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt gtttaaagag
    46621 attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt agaagaggaa
    46681 gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg aaaaatccaa
    46741 gctcgagtcg atttcgtcta tggtgaagtc atctattcga ctgacccgaa gcatgaggtc
    46801 tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg agtacttgat
    46861 ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc attgccaagc
    46921 ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca gtttggtgaa
    46981 gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca tcaaccgaaa
    47041 atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac agggatagag
    47101 gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt ttatacatta
    47161 gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag ccaagtgttg
    47221 caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc taaaaatcaa
    47281 ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga atcgttccaa
    47341 acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc caaaggattg
    47401 aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat
    47461 tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact acagacaatt
    47521 gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat cgtagcgcct
    47581 gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc tttaagtatc
    47641 caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga cacagatatc
    47701 cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc gcaaaaaatt
    47761 gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa
    47821 gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc aatcgagaat
    47881 aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt cccgactaaa
    47941 acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc gtttatttta
    48001 cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat gaatctatat
    48061 tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa
    48121 gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat acttgctggt
    48181 ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc
    48241 ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga gaacaaacga
    48301 agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga agagttagcc
    48361 gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga tcggttaagt
    48421 atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg
    48481 ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct ttggtggaat
    48541 cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa aaatgtggta
    48601 gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc actccagcaa
    48661 gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt gacgaaactg
    48721 actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta agagactcga
    48781 aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt atttgattta
    48841 aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat cattttttta
    48901 tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc tgatcaaaag
    48961 aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga tggttgaacc
    49021 aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa
    49081 cgtataaggt ctcttctgga agcatatgaa attctttctc agccagctga aaaatctctt
    49141 tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa ggaatccaat
    49201 tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca tttgtgataa
    49261 ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc tcctcatgca
    49321 agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg
    49381 taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct cgcatatatt
    49441 ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga tggcggaacc
    49501 ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt
    49561 ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca actgatttaa
    49621 tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt gactattgta
    49681 acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat atttctattt
    49741 ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt gaaacacgtc
    49801 aataagtttg atgaacaaat taatcattat ggtagaagtc gattgattta ttttaatgca
    49861 atctctgtgg aattttcgaa agatttattt ttttaataca gtctcaatat aactattcaa
    49921 acataaaata acgttatttg atttatttgt ttatagtaaa ttttatttag ta
//
LOCUS       DACDCV010000009        47713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDCV010000009 DACDCV010000000
VERSION     DACDCV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.009"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..845
                     /locus_tag="IV041_000554"
     CDS             57..845
                     /locus_tag="IV041_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8381374.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            832..1158
                     /locus_tag="IV041_000555"
     CDS             832..1158
                     /locus_tag="IV041_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381375.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            1507..2547
                     /locus_tag="IV041_000556"
     CDS             1507..2547
                     /locus_tag="IV041_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8381376.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            3383..3715
                     /locus_tag="IV041_000557"
     CDS             3383..3715
                     /locus_tag="IV041_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8381377.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4306..4635
                     /locus_tag="IV041_000558"
     CDS             4306..4635
                     /locus_tag="IV041_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381378.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            4622..4963
                     /locus_tag="IV041_000559"
     CDS             4622..4963
                     /locus_tag="IV041_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8381379.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5077..5316
                     /locus_tag="IV041_000560"
     CDS             5077..5316
                     /locus_tag="IV041_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381380.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5383..5622
                     /locus_tag="IV041_000561"
     CDS             5383..5622
                     /locus_tag="IV041_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381381.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            5708..6379
                     /locus_tag="IV041_000562"
     CDS             5708..6379
                     /locus_tag="IV041_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8381382.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            6432..8408
                     /locus_tag="IV041_000563"
     CDS             6432..8408
                     /locus_tag="IV041_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8381383.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            8421..9077
                     /locus_tag="IV041_000564"
     CDS             8421..9077
                     /locus_tag="IV041_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8381384.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            9173..10000
                     /locus_tag="IV041_000565"
     CDS             9173..10000
                     /locus_tag="IV041_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381385.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            10010..10258
                     /locus_tag="IV041_000566"
     CDS             10010..10258
                     /locus_tag="IV041_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381386.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            10289..12400
                     /locus_tag="IV041_000567"
     CDS             10289..12400
                     /locus_tag="IV041_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8381387.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(12438..14495)
                     /locus_tag="IV041_000568"
     CDS             complement(12438..14495)
                     /locus_tag="IV041_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8381388.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(14609..14821)
                     /locus_tag="IV041_000569"
     CDS             complement(14609..14821)
                     /locus_tag="IV041_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381389.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            15008..15475
                     /locus_tag="IV041_000570"
     CDS             15008..15475
                     /locus_tag="IV041_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381390.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            15472..15714
                     /locus_tag="IV041_000571"
     CDS             15472..15714
                     /locus_tag="IV041_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381391.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            15732..16034
                     /locus_tag="IV041_000572"
     CDS             15732..16034
                     /locus_tag="IV041_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381392.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            16105..18159
                     /locus_tag="IV041_000573"
     CDS             16105..18159
                     /locus_tag="IV041_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381393.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            18159..19991
                     /locus_tag="IV041_000574"
     CDS             18159..19991
                     /locus_tag="IV041_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8381394.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            20686..22602
                     /gene="ltrA"
                     /locus_tag="IV041_000575"
     CDS             20686..22602
                     /gene="ltrA"
                     /locus_tag="IV041_000575"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8381395.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            22719..23519
                     /locus_tag="IV041_000576"
                     /pseudo
     CDS             22719..23519
                     /locus_tag="IV041_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            23516..26047
                     /locus_tag="IV041_000577"
     CDS             23516..26047
                     /locus_tag="IV041_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381396.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            26097..26429
                     /locus_tag="IV041_000578"
     CDS             26097..26429
                     /locus_tag="IV041_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381397.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26430..27023
                     /locus_tag="IV041_000579"
     CDS             26430..27023
                     /locus_tag="IV041_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381398.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27186..28880
                     /locus_tag="IV041_000580"
     CDS             27186..28880
                     /locus_tag="IV041_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8381399.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            29568..31445
                     /gene="ltrA"
                     /locus_tag="IV041_000581"
     CDS             29568..31445
                     /gene="ltrA"
                     /locus_tag="IV041_000581"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8381400.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            31576..31953
                     /locus_tag="IV041_000582"
                     /pseudo
     CDS             31576..31953
                     /locus_tag="IV041_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            31953..33158
                     /locus_tag="IV041_000583"
     CDS             31953..33158
                     /locus_tag="IV041_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8381401.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            33174..33818
                     /locus_tag="IV041_000584"
     CDS             33174..33818
                     /locus_tag="IV041_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381402.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            33829..34635
                     /locus_tag="IV041_000585"
     CDS             33829..34635
                     /locus_tag="IV041_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381403.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            34657..34887
                     /locus_tag="IV041_000586"
     CDS             34657..34887
                     /locus_tag="IV041_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381404.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            35433..35708
                     /locus_tag="IV041_000587"
     CDS             35433..35708
                     /locus_tag="IV041_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381405.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            35750..36172
                     /locus_tag="IV041_000588"
     CDS             35750..36172
                     /locus_tag="IV041_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381406.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            36191..37783
                     /locus_tag="IV041_000589"
     CDS             36191..37783
                     /locus_tag="IV041_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381407.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            37795..38130
                     /locus_tag="IV041_000590"
     CDS             37795..38130
                     /locus_tag="IV041_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381408.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            38277..38471
                     /locus_tag="IV041_000591"
     CDS             38277..38471
                     /locus_tag="IV041_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381409.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            38503..40875
                     /locus_tag="IV041_000592"
     CDS             38503..40875
                     /locus_tag="IV041_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381410.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            40936..42396
                     /locus_tag="IV041_000593"
     CDS             40936..42396
                     /locus_tag="IV041_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8381411.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            42407..44611
                     /gene="topB"
                     /locus_tag="IV041_000594"
     CDS             42407..44611
                     /gene="topB"
                     /locus_tag="IV041_000594"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8381412.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            44857..45450
                     /locus_tag="IV041_000595"
     CDS             44857..45450
                     /locus_tag="IV041_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8381413.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            45466..46365
                     /locus_tag="IV041_000596"
     CDS             45466..46365
                     /locus_tag="IV041_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8381414.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            46368..46853
                     /gene="ssb"
                     /locus_tag="IV041_000597"
     CDS             46368..46853
                     /gene="ssb"
                     /locus_tag="IV041_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8381415.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            47219..47479
                     /locus_tag="IV041_000598"
     CDS             47219..47479
                     /locus_tag="IV041_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381416.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT        17649 a         7499 c         9671 g        12894 t
ORIGIN      
        1 atcacaatat tatgatataa ttatattata atataagatg aatgggggat attttaatgg
       61 caagaatatt gtcagtagcc aatcaaaaag gtggagtttc taaaactacg actagcgtta
      121 atttagcagc atgtttgaaa tttgtttacg gtaaaaatgt acttttagtg gacattgatc
      181 ctcagggaaa tgctacagat aatgtaggtt tcgatataga tggaacaaac caacctacaa
      241 tttatgaagt attaaaaggg gaggctgaca ttacagatgc tattctggac tataaaggaa
      301 ttgatgtttt gccagccgac atagcccttt cttctgcaga gagggaattt actcaagtag
      361 gaagtgaaca caggcttaaa agagtacttc aaccaatcaa ggacaactat gattatatta
      421 taattgattg tccaccaagt ttaggaatgc ttacagtcaa cgcatttact gtttctgatg
      481 aaattatcat tccagttgaa gctgcgtatt tctcattaaa aggactaata aagttaagtg
      541 aaactattga aacggtaaaa gaatatacga acgacaagct tgaagtacga ggagtcctat
      601 ttactaagta taataagcgt tataacattt ctaaagaaat gacgactgca gcaaccgaaa
      661 tttctagagt cattggtgca aaaattttca aaacttatat tcgtagaaca ataaccgttg
      721 acgaagcgca agctgctgga tcagatctaa tcaattttaa taaaccctca acagctgaag
      781 atgactataa agcatttaca gaagaatatt taaaagaagt tggtgatttt gatggcagaa
      841 gataatcgca gtcaagctgc agtaagtaga ctactaggaa ataaaaataa aaagaactac
      901 aaaccaaaag aagtagaaga aatcgaatta accaagaaaa atataagtaa agtcataaaa
      961 gatcaaggaa gtagtgtaag aacagcagat agcattcgta taaatccaat tctaatgtca
     1021 gcactcaaat attggacgac tattgctgaa tcagacaaaa gtaaaccaga cgttatagaa
     1081 gatgcgctat taaaatccat acctgaagaa tacttgattg aaggatataa actagcaaag
     1141 aaacaaaata aaatttaatt tacattcaat atcacgatat cataatatcg tgatattttt
     1201 tttataaaaa tcagtagtat caaagctgat aagtcgctta tcttcatatt gtaatattac
     1261 gatatcgtga tataatgtta ttgtaatatc gtgatatagt taccatgagc ttaaaccctg
     1321 aaatagagcg gtttgcttta atttattttt taaggtaaac taaaaaaaga taaaaaaaga
     1381 ctactgcttt aacaaagttt ggcgacttat cagcagtaga aaaatatata gtggatagat
     1441 atatttgttt aattcattgt aacatagttt tgaacaaaac gctatcctac agaaagagga
     1501 ttggctatga atgattttaa ttattacaaa tcaaaagaaa tttatcgtga aaaatattat
     1561 caaatgccta aggtattttt tactaacgaa aaatacatgg atctttcaaa tgatgcaaaa
     1621 attgcttata tgctacttaa agatcgattt gattattcag tcaaaaataa ctgggtagat
     1681 tctgatgata atatattctt catattcaca gttgaagaat taatgaaatt gttacaatgc
     1741 cgcgaaggta aagtatctaa gatcaaaaaa gaattagaaa aagcaggtct tctgaaacag
     1801 aagagaggaa gagtaaataa acgtgatggt aagattgaat ctatgcctaa tttattgtac
     1861 ttaggcaagc cagaagttac gaatcaagac gtctttaaaa tcatggaaga ggaagacaat
     1921 actgattcca ccgtaattgc gaaaatcgca aatacggcaa aaccgcgaaa aatcaatgat
     1981 tctaccgtaa ttgcgaaaat cgcaaatacg gcaaaatcca gtcatatcaa ggactcaacc
     2041 gtaattgcga aaatcgcaga caatctattc tattctaatt ctatagacac taatagacac
     2101 ataatagaca ctgaaactga ccagttacaa aatcaagttt tgctagataa cttcgtcgac
     2161 atcatgcaag aggacagtat aaatacattt gtaccagaaa atgtattaaa cttgataaaa
     2221 acattttctt caagttacag tgaagctcaa caaaccgtcc aaactattca taatgcaaag
     2281 aaaaaagctg aagaattaga gcaaactatt gtcgtttatg aagaactgga atcttatgga
     2341 attgatgctg acaaaggatt atatatgact ttactgaaag cttatcaaaa acaaaaaact
     2401 gaaaaagtaa acaatttgca gaatctaatt ttcgtttatg taaaaaattg gtttgtcgaa
     2461 aaagctattg cggcaaagct tgcaaacgaa caaagagaaa catttaacga attaccaaca
     2521 gttagtacgg aaaattggtt agagtagttg ataaaaagca attactataa aaatgatcaa
     2581 ttttaaatta gaactttttt atgattttaa ttttattaca aatattacaa ataaatagtt
     2641 agtattccgg agtttttaga ttaaaagttt atgaaaacaa tcacaatcgt aggttttaaa
     2701 ttcataatcg ttagatattt tagttatttt tcactcaaaa aaatagggaa ctagtgtttg
     2761 catatgtgct tttttgagtt ataataattc atgaagagag gtaattcact tatttcccca
     2821 agctgaaaag cctgatatta cttcttttca ggcagtgctg ctaatattca attacacttt
     2881 tttgtatatt tttagttgtt ttaaatttaa ttgtctgcta tgcttgtgat gaattgattt
     2941 tcgttctgca cttttggacg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcc
     3001 ctgtgagact cggaaggctg gtaaccttcg tcgacgtcca caggtggcat taagatcact
     3061 cccttaatgc gtttaccttt cagtgttacg gtattacccg taacggattt agtataacag
     3121 atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac
     3181 gatgaatcta tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga
     3241 cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat
     3301 gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta
     3361 aagtaagtgg gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg
     3421 tcactacggt aaacggtgta ccgctacaga aaagatcacg aagaaactat caactggtca
     3481 aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagaaga
     3541 aatgatttat tacattggga aacaaaaaca aataatttta aaagatccta tccctttaaa
     3601 agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag
     3661 aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttaatca gctgaattga
     3721 tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc
     3781 taaaggaact tagaatcact agaaaaataa ggagtagact tgatctatca acaattcctt
     3841 tcggggggtg acggggtagg tttttacgat tggtaacgta aaaaattggt caatcgagta
     3901 aatgcccttg tttggatagt ggtgaaatga atttgtgtaa ataatatgca cagagtaaac
     3961 aagagggaat ttagaagtta gacaagttag aatagcgcct agattaatca tgagcagcta
     4021 ctaagggatc taggctttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg
     4081 ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaagacgt
     4141 tgatctttgg atcaatcgag catttaccgt tttctactag ggtttctcgt ttcggacgcc
     4201 ataaacgagt cgatttattt tatatatttt tgagtgaacg aaaaacgata aaaatattcg
     4261 ttatgaagta aaactaattt agtagatagc aaaggtggta gaggaatgat tcaaaaattt
     4321 gcgaaaatga gtaaaactgc atggattgaa aagcttgaaa atgacttgag tgtcatgcga
     4381 ttagctccaa agaaattcaa acgagagatt gcagaaaaag aagcaatttt gtcggtattg
     4441 aatgcggtag acgataaatg tccagtaaaa attagttttt cagatatagc aacaattatc
     4501 gaaggtattt attatggtga agttgatgaa gagatatctt ctacaaaaga tttattagat
     4561 tttttggata aaaacaaaga tacctttata gtactaactg attatgagga ggttacaaca
     4621 aatgaaaaat tatgatccga atagccgatg gggaacacat acgattaagg tttctttcca
     4681 acgttgggat tacaaaggat ttgtgacgtt tagaagaggc ggaaattgta aaggattaga
     4741 tgttttagca ttagacgagg atgatttata cgatcaaaaa ttgactgata atccaattgg
     4801 attcggcttg ctacctgaag atgatcaagg taatgaatgg tttaaaatga cgcttatgaa
     4861 tgataatgga gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt
     4921 cagcgttgaa atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat
     4981 tttacagata aaggcttaga aggagtcacg acgattaata aaaaggtagt attaccttag
     5041 gcattgagta aggaatttat ttaggaggaa aattagatgg cgcgaccagt tttagaggcg
     5101 atggatatgg atactttgac aaaaggaagg tatgtacagc tgttagctcg tggttattca
     5161 cctaaagaag tattcaaatc cctgtctaaa ggaacagata tggaaaaaga aagattacga
     5221 aaagaattcg attattggag aacccataat ggaatcaagg atttgaaacc agcacggcca
     5281 gtaattaaac gaaagaaaaa gataacccaa gactgaagtg tagagcgtat gtgatcatag
     5341 gcttttttat ttttgcaaaa aacaaggaag gtaggaaaac tcatgaatta tgaagaaatc
     5401 gaaaacagaa aaaaagtgtc gaaagaaatg gaagaaaagt tgctaaaaac gatgaaacaa
     5461 aaacacctta aaagattatc tgtcatgcaa tatatcaatg atatgcagat aacaggaaaa
     5521 gagaaagctt gtttgttagg ttcaatgaaa aactttgaac agttgagacg aacttatgtg
     5581 aaaaaaagtt ctaattgtca actattgtta gaagtctcat gacattcatt aatagaattc
     5641 atttttaaaa ctttttctat ggaaatgaga aaaagatttt ttgttttgtc tgaaaggggg
     5701 tagctggatg agaaaatggt tatttggttt tttaggagtc gctttaatcg tcttttgcag
     5761 tgtgtttggc tatgttagct accaaaaaca tgaaggaaag gtgtttaagc aaaatataga
     5821 aaaaaagatg cccgtagatc aaatcaatga acatgcaaag agttataagg aggatgcaac
     5881 gaacgtcaat aatgatatgt ctttggggca aatgttgtcc atacaaaaag aagcaatcga
     5941 aatgggtgtc aataaacaag cctttgcaca aattcagata cctgcacttg gtttagcatt
     6001 gccaattttt aaaggggcaa atcaatacac tttgagtctt ggagcggcta cttattttta
     6061 tgaagatgcc gaaatgggca aaggaaacta tgtcctagca ggtcataaca tggaaatgcc
     6121 aggtgtttta ttttctgata ttcaaaagtt gtcattaggg gaggtgatgg acttagttag
     6181 taacgatggc gtctatcgtt acaaggtaac gcgtaaattt atcgtacctg agtactttaa
     6241 attaatcgat ggcgttcctg aagagaactc tttcttgtct ttaccaaaga aaggagagaa
     6301 acccttatta acgttgttta cttgtgttta tacgtcacaa ggaaaagaaa gatacgtggt
     6361 acagggagag ctacaataac aaggttttaa tcaacgcgag tcgttgaaat atgaaatgag
     6421 gagagtaaaa aatggcaata aatcggtatg taaaaaaagc tggaatgctt gttggaatgt
     6481 taggaatcat tgcaagctgc tttcaggggt atcaagtaca cgcagaagat gtcacgcaaa
     6541 aaacaccacc agagaaggtc aatatcacgg ttcataaatt aatgtacgat caaggaacgc
     6601 aattgaatgt tgatattgac gggatcaaaa acgatggtta tacgcacgat caatatccta
     6661 caggtgtaac gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata
     6721 ttacggatca agtattaccg agagaagatt ctgatctaac caacgcgaaa gtggacgaga
     6781 ttgtacaaga tgtagaagac aagggatcta acagtgagta cgtcaagaat gcaaccaaca
     6841 aaattacttc agctgtagat gaaaatggtg agatcacttt tactgatcaa ccagcctatg
     6901 tcaattccaa agggaatgta tatgtggttt atgaaagtaa atccgctgct ggattagtca
     6961 cacaaaaagc aaaaccaatg gttgtgattg cgccaatgac agataaaaca ggatcaggat
     7021 ttttaaaaaa tattcatttg tatcctaaga acattgtttc taagttgagt tttgaattaa
     7081 cgaaatttgg tgacgatggc gcagcccaaa gcaaacaaac ccttttaaaa ggggcaaaat
     7141 ttgagttata caagggcgaa cctggaaaag gaacaaaatt gggtgatctt gtatcggatg
     7201 atcaaggaaa gctgactgcg actgatttga ctttaggaaa atactatttt gtggaagttc
     7261 cttcggaagt tgtagtggga tcagatcaag aaccaacagc agatcaatat ttattaggcg
     7321 cagacgctcg caacgatgcg cataataaat taacctttga aattaccaat gacggcgtga
     7381 caagtgactt aaaagcttcc tacgtgaact ataaagcacc agtgatcgat aaaacagtga
     7441 caaatggaac aggacaagaa cattctttcc aaattggtga cgcagtgaat tatcaaggaa
     7501 cgattcatat ccctacagat attgcaggtg gaacagatgg gattacagta aatggtgtga
     7561 agagtgaaac ttctccttac agcgtattta agtggggaga tacagcagga caaggacttt
     7621 cttatgtcgc tgctaaagca aatatcaaag taacaaataa agatggatct gtggtattaa
     7681 aagaaaatac agattataaa atccaaaatt cagaaaatgg ctttgtgatc gattttatcg
     7741 tgaataatgg ccaagtaagt gatactgtag caagtttaca cggacaagat cttcaaatga
     7801 cttataacat gtatgtaaat gacagcgcag ctgttgccaa tccattaaca aatagcgtgg
     7861 atttcgttta taacaacaat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg
     7921 acgttgtgac ttatggtgca aaattcttaa aagtcgatag cggactattt ggaacaggaa
     7981 tcaaagcaac accgcttgaa ggcgcagaat ttgctgctaa aaacgcagaa ggaaaatatt
     8041 atggtggatt agttgatacg gataaagacg gtgtcaaaga agcagtatgg gtagacgatg
     8101 tagcaaatgc agcgatccta aaatcagata aagaaggtca ctttgaaatc actggtttaa
     8161 ctgaaggtga atattcactg gaagaaacaa aagcaccaac tggttaccaa aaattaacag
     8221 aagatattgc atttaaagta aacaaaaact catttaaaga ggaaaatcga atcactgtta
     8281 aaaataatca aaaagcagct attccattaa caggtagtaa tggtttccaa acgtatgtat
     8341 tgatctcttg cttgttgcta ggagcagggg cactttctgc agttgtttac tttaagaaga
     8401 aagcctagaa ggatcagcta atgattattc gtaagccccc aaaaagaaga gtcatgcaca
     8461 aactgatgat atttttttgg ttgctgtcgg cagtagcagt ctatttaatt ccagctgtgg
     8521 aaacaaatgt agcgaaattt caacaacagc aaagaatttt agccattgaa aatcttgaaa
     8581 aaacagattc ttctttggat gttgcgaaaa tagagttagg cgatcctgtt ggtgttctta
     8641 caattccaag tattccttta aaactaccaa tttatgacgg aacttccgac aaaattttag
     8701 agaatggtgt agggattacg gaaggaactg gcgatattac aggaggaaac ggtaaaaatc
     8761 ctttaatcgc ggggcacagt ggactttata aagataatct tttcgatgat ttgccttcag
     8821 taaaaaaagg gcaaaaattc tttattaagg taaatgaaga acaacatgcc tatcaaattt
     8881 atcgaattga agaagttaaa gcagaagaat tacaaaaaaa ttatttagat tatctcatgc
     8941 ctgaaaaaga ccaagatagg atcacgttaa tgacatgcac gccaaaaggt gttaactcac
     9001 accgcttttt agtgtatggt gaacgagtgt cattcagtaa agaagattta aaactacaga
     9061 aaaaagcaaa aaaataggaa atcaatttca ttggattggc gattagcact gctattcgct
     9121 ttttttgcgt tcctattatt tttatttata aaaaaataag gaggagatcg gaatgaataa
     9181 gagaaaatgg ctgtttgttt gtgtagcagc tctctttgca actgcagctt actcacagat
     9241 atcttttgct gatagcagta ctactccaac taccagtcag acaacagata gttcaacaac
     9301 tgcaacaagt gacacggaca gttcgaagaa cactgcagaa gaaacggcag attcaagtac
     9361 gagttctact gaatcagcgg aatcaacgtc agaaagttct tcagagactt cagaaaaact
     9421 gcaagaagta gaagatacgc aagtaatcaa gcaaaacatt catattcaaa aaattatttc
     9481 ttcgaatcga atcaacaatc aaggaaggca agtgactcag caagaaggtg tcaatggtgc
     9541 aaagtttatt gtctacgata tcacgaatat cttagaagaa atgacacaag atgataatga
     9601 gaaaaatcaa tcggtagaaa taattgagtc aaaattgaaa gatcgagcaa aaaaactttc
     9661 atacgatcag ttgaaaaaag tgactgaagg agaaacccaa acaattgatg atcaggaagg
     9721 cgtgattgat ttttcaattg aagtacctgc aaacaaaaaa caagcatact atatcgtcaa
     9781 tgaatcaagc cctgaaaaca tttcaaatag cgaagatatt gttttattga cacctgtttc
     9841 agatgaaaag ggtgaatttt tgacggatgt ttggttatat cctaaatcaa agaaatcaga
     9901 gccgaaagaa gaaattaaaa aagttgtatc aactggtgta aagaaaaact tttttgataa
     9961 ttgttgggat tttttcactc atttattttt taacgattag gatgaaatga tgaaaataat
    10021 aaaaatgtta gtttttaatc tatctttttt agccgcaaca atattgtgtt gcggtttagt
    10081 tttttctcaa ttatcaattt acccaagata tcaacgattg ttcgttcatt ttctaaatat
    10141 ccagcatgct tccatgccac tagaagtgat taacaagctc ttaatcaatc caatttttat
    10201 ggctagtttg tttcttcttg caggattggt aacgtgggta ttaggaaaaa aagaatgaga
    10261 agcttacaat cgtttaacaa aataatagat ggagggcatt atggaaaata aaattcattg
    10321 cactacggga agagtattta ctttagtgtt gtttttgttc cttcttcttt ttgcaagggg
    10381 aactgcagtc caggccttag aaaaagttga tccgagtcaa atcaaagaaa ataataatct
    10441 ggtactttat catgatagtg attcattgag aaatatcgaa gtaactggga tcaatggatt
    10501 taagatacta gatgagtatt caactaaaaa acaaaaagta cttgcttggc caaaagtcaa
    10561 agatttgaaa agcacgtcaa aaattgttgc ccattatcaa aaggtgggca cttatctagg
    10621 aaaagaaatt gattgcgacg taactttttc taattttgta gcgcacgctg gggttaacca
    10681 agacggatta gctaaaacaa ttaatgggaa aaagtattat aattatttgc tctttgataa
    10741 aaacatttat cgaggctttt tcatgcagtc aatcaacgtt atgaatgtta aactagattt
    10801 taagtatgca ggatcaaaaa caaaagtagc tttgagtgaa ccacagccag aaattaattc
    10861 aaaaatgaga tcgatgagag cagctggtat tgagttgaat gatacatttc tatctattta
    10921 cagtttaaat ggacagtatc agcaacgtgt gtcttggtat aatgacaata atgaatctgt
    10981 cggtgaacgt aatattgata tgagagaatt tgttgggtac gaaaaaatgg atcgcttacc
    11041 ttattttgtc acaacagata cagcatgtgc agaatacaaa gctcctgcgt tatcaacaaa
    11101 caatttaact tcaaaagtag tgggaggacg tgcagaaaag gcttatagct cgaatgatca
    11161 tttcaccgat gttgtaggag ctgatactta tcacagaagt ggtgtaacgt atacgcttca
    11221 aggcgcttcc ccaacattca tgattggcgc aaatacgaat agtatgatgt ttagctttga
    11281 tactgcattg ctttggacac cacaaccatc gaagcctaca aaagaagtgt ttaacaaagc
    11341 taatactgaa gaggcagcac acaatattga caaaaaagtg attccacaag gatcagatgt
    11401 ttactatcat attcaccaaa agtttgatgc attaacagtc aacacaatga acaaatacaa
    11461 atcatttaaa atcactgata cctttgacag caaaaatttt gatatggtat cggatgggaa
    11521 aaactatgat ggcgcattgt ggtatgttga agcgaatggg acttggaaaa aagctgatac
    11581 aggaggaaaa ttaactgtat caggtaatac agtcacttat actgcttctg acagcttttt
    11641 agctaatgct gcaaattacg gtagggacta cattttgcag ctccatatga gagcaaaaaa
    11701 tgatgcaaaa ggaattgcac caaatgtggc tactgtaacg tttaacaatg atccgaacaa
    11761 aggaacacaa aaaacaaata aagtagaaaa ctatttctct cttaaaccaa ataaaaaagt
    11821 ggaacaagat gggaaagatg ttgatggtcg aaatgatggt aaaaaaggaa cgcctacagc
    11881 tcctttaaat gcaggatctg aagtacaata tctagtcaca caaaaatggc atacaaaagg
    11941 tgtagacatt gcgctcgatc attacaagca atttagtatt caagatccag tggaagctcg
    12001 tttgacgtat aaagaaggtt ctgcgcgagt gatagatatg agtacaggaa aagatattac
    12061 ttcagaaggt atactaacct atgatagcca ttcaagaacg ctgaaatggg aagcttccgc
    12121 tgatttctta aataacaatc ttttagatgg acgagaaatt caactgatat ttacagctaa
    12181 aactcctttg aatgaagaat tggatatcga caaccaagca aaagtttcag tagaaagtat
    12241 tgagaaagca actaatgtag taacgattgg ggtaaaacct aacttacctc aggtcattgt
    12301 gccaaaaact ggatcgaatc atttagtgct aactgttatt ggaagtatat tatttcttac
    12361 tttagcatca ttgggctatg tagttttaaa actcaaataa atataaaaac aaggctgatt
    12421 ttcagccttg ttttctttta tttgaagatt ggattcgcac taataaatac attatctacg
    12481 ccaattctac cagcagagtc aactacaaaa ttatgaccta atccgagaat actttggtta
    12541 ttataaagta aatttgcatt atgggcaggg ctatttttcc atcctaccat ggcatcgata
    12601 gcaccatcgt ctgataaaag gtcatcaata tacagcccag tttgacttat gttttctccc
    12661 ttagcaccat agcagcctaa gctttccaat aaatcttgga aagtaccacc accagggcga
    12721 tagtgactga agctgacttg taattcttta gctcgaatat ctgcagcctg ttgaacaata
    12781 tcattgaatt gcattggggc aacaccattt tgttggcgat aatcatttaa ccaagcaagt
    12841 tctttttgac gcataaagtt acgtaattcg tctaatgaaa tctttttata tttgaatgtg
    12901 attgttttat tttgatcgac gttattaatg gatcgttggc cgtttttatc cacggtaacg
    12961 gtaggatcgt catttctgca caagaaataa ttttgatcag tcatattttt ccaaggagta
    13021 gagaagctat ccccttcgta tactgttttt gattcactag agagaacagc gttggtatca
    13081 gcatttacat gattaacagt cacattaaat ttacgagtgt agttgaaaat ataattttga
    13141 ctttcagtca cattgtttaa ggttactgaa tttgttggtt ctacccgatc gtctaaaaca
    13201 tatccttgaa tagaaggagc agttaaagtg acactttcac cgtgagtgac gttgattatc
    13261 tgttctttca aaatatcaac tcgcccctca tttttgtagt tgttattgca caacaaattg
    13321 atttgatgca cttgattata attgaatgtg attgttgtat tacctgtgat tgttttgctt
    13381 tgagtatcat taccaatcac tttgtagcta aaattatctt tagcaccttc gctatattta
    13441 aatgcttttg ccttagcagt gtaagtagaa ccagaagtca cttttgcttg atctgtagca
    13501 atcacttgac ctgtttgatt gtcaatatgg ttaatggtaa ccatatattc ttttggcgtt
    13561 tcaggtttgt gttgcaaccc tttaaatgca gcatcaagac tatttttagc attgtttact
    13621 tgtgtttgag aaaccttaga atcgtctagt acttttttag cattggataa agcagtctga
    13681 aaattattcc aagattcttc tgtaaaatct cctttagcag ttgattttac tcggttgtat
    13741 aaagcttgta attcagcttt attcacagta ggaactggtt ttttctttaa tcctttgtaa
    13801 gcagaatcta atttattttt ggcactatcc acttgctttt gagtcgcttt tgagtcattt
    13861 aacactttct tagcattgtt taaagcggtt tggaatgcgt tccaggtatt gtcggtatag
    13921 tcaccttttt tcgttccctt aacgctcgta taaagagctt ctagagccga tttattcact
    13981 ttgggtttag aggtgtcagc gccataccaa ccgatacctt cgtatttcca gccatagtct
    14041 ttggttaacc attgtacttc tttccaactt gcagtgtagt gatgtgagcc agattttaca
    14101 ccaggaagga aaaggcggta gatcggttta ttattttttt cggcagaacg ccaggcaggc
    14161 ccttcataag tccagccgta atttttagtc aaccatttca cttcatccca actagctgtg
    14221 taatggtgat cacgaagacc tttattgtac aaacgataga caagatggcc accgctagtc
    14281 ggtgcttccc aggcggggcc ttcataattt ccccatttta tctttactag atggtcacgt
    14341 tcttttttgc tagaggtgta aaaatgttca ttattatttg gattgtaaag gcgatacatg
    14401 acgttagttg aagcagcgtc aacagatcca gcaagactaa gtgaggcagc aaatgttcca
    14461 atagctaaaa cagtttttgg attaaataat ttcatttttt caactcctaa atttaattaa
    14521 aaatgtttac atgattatta tatatcggtt aaccgatata gtcaataaaa aataagaaaa
    14581 gccttatata attttggctt ttcttatttt atttgttagt tgaagtatta atatcaacga
    14641 tttcttcagc caatttattt aatttcatag catttatgaa tctcatgttg tctatactag
    14701 agcgtttgtt ggcaaggtca cttactgtag ttaagggtac gccagattct atggatatag
    14761 aacgaactga tttaccacta tcaaacagca accattcgat tttcttttta tcagcgtaca
    14821 tttcaatgct ccttcttgtt ttttctttat tatagctctt ttttgtaatg aatcacaata
    14881 tcaagagaaa aattttctat agaaaggtgg atttttatga gtattcgttc tccgtgtatc
    14941 caaatgaggt ttgttgctac tttcgttatt tgatatacta aaaataaaca acgaaagaag
    15001 gaataaaatg aagaaaaagc aactatggtt tgtctctatg tttgcaatat tttttattat
    15061 tttaagtgga tgttcaagta ataagaaatc ggaagctaaa gaagataata gtttattgca
    15121 accaggaact gtttggaaag aagaagtagg tggtcttatt cataatttaa aaattataga
    15181 tgatactacc tgggaatatt ctgaaagtgt atggcatcca gatcctgtac aaataacagt
    15241 taagaggcaa aaagactatg aaggtttaga aagatacaaa atagtagatt ctgcaggcat
    15301 tagggaattt ataaacaaac ctgattcatt atttattgtt gttccctatg agaaggatgg
    15361 cgtaaaaaaa ataatttttc ttggatctag caaggatgag aagcgaacga aagagaaatt
    15421 aattcatgat ggatcacaat caaataaata caaattacaa aagactagcg agtgattgct
    15481 agtcttttta tttgtcctga aggaggaatc acagtggcaa aagctaaaac gccatttgaa
    15541 aaagccgcgt ctaaaatctt agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa
    15601 gtccttgaaa aagcacaatt tgacctatta cggggaaaag acaaggactt agaaggatat
    15661 atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag
    15721 cgaacaattt tatgtttgaa tcaattacaa tgaatttttt aacacaaccg aaagtagtag
    15781 ctgcggttga tatttttaga gcattacaaa ctgccgcaac tagtttgaaa aataatctga
    15841 tgatttttgc tgctgttgcg gcagtggctt ccattgctct tggaggtatt ttcttcttct
    15901 ttgggcgaag aggagctgaa aacgggaaga cacacgtagg ttatgtattg ttgggggtag
    15961 gagttatttt agctgccgct tctattgtag tttcaatgtt ctcaatgttt ggacaaaccc
    16021 cacaaggact ataaaaaaac aagtagaagg agtaaaaagt acgtggctac tttttactcc
    16081 tttttttatg gaaggagaat attcatgttt tttacgattt caggcaatgc aaaattggat
    16141 aaagagttaa taagaaaagc aacgaagaaa gtgggaaagc gtacttcctt tttaccgaaa
    16201 acaattaaat attctgtcat tgaaacggaa gaacagctac atgtgttagg acaagtgtta
    16261 gaacaagaga atcaatcttc tgttaaattg actctcacgt tacatgataa agaaggaaaa
    16321 atagaaaagg ctggcacaga atacgttttt ggctcactgg attatcaaat ttcagaaggt
    16381 ttttatccta cattgattga tttaatccgc ctagatatat ttaaagatac ggatgaaacc
    16441 atgagtgtgg aagagtctta tcgaaacgaa aaaattttac aagttttaga agaagagtta
    16501 gaaagcgacc aaagaaaagt gaatcaagtg agtgaagtcg aagcaaacga agaagtacca
    16561 tgggaacgcc caatctttcc aaaggatgaa tcaaaagaaa aagagaaagg aattattgac
    16621 gaacaagctg acattttgac tcataaatca atatttgagg aagagaacga ggtaatgggt
    16681 caattggagg tagatccctt tgaagaaaca atggaggatg aagagacggc cgaagtagaa
    16741 gttgatccag ttgaagctaa tgaagaccca acatggactg aaggggattc tcaggaatat
    16801 attgaaccac ttatcagtga agaagtagag aaaaatgaaa cagagataca aaaggcaacg
    16861 gtatttgatt atattttgga tcaatatccg ccagagcatg aatggatgaa agaaaaaaat
    16921 aaattatttt taaatgaact ttattcgaaa tatcaattac ctcaaaccta tcaagcattt
    16981 ttagagagta aagaagaatt aatctcacag ggtaaaggca cacttttaga acagttagag
    17041 tctgtccaat caacaaattg gcatgagctt gcacaagaag atttattagc tacttttgaa
    17101 gaacgtcaac aagaaacaga tcaagaaatt aatgagtatg cgacggaaca gtcgtcgaat
    17161 tgggagaaag caaaagagga actaaataaa gaagaacagc gagtgattga agaagaagta
    17221 caaaaaatta aagagcggta cgaaaagaaa cgtgacaatg cgtattcctt gtgtgtagaa
    17281 aagatcaaca attttactca gacgaatcaa gaagcattat cgaaagaaaa agaaaactta
    17341 ttaaaagaac gtataaaaga acgaaaagaa gaattttacc gagggttgcg tactgataag
    17401 ctaaggatta gcaaagaatt aaatgaacaa ctgaataaac tctatcaaac tacgactgaa
    17461 gttctgatgg aaaaacacga gcagatccaa caagaattac aagcacaaat tccaaaatgg
    17521 aaaaaagagt atgaaattca ccaacaagaa ttgaaagaac aagaagaaaa acaacggaaa
    17581 aaagaaaaag agcgcgaaca attagaacta aaaaaacgtg aacaggatct tgcggaacaa
    17641 aaaatggcga tagaaaaaca acggctagaa caagaaaagg aaaaagaagc agctgctcgt
    17701 aaggaacgtc aagaacaaat ggatacattg cgacaagcac agttaaatta tctgacacat
    17761 cctcaaccag tagctgctgt tcaaacgcca gtgaacaacc aagcaaattt aactccttca
    17821 ccccagcgtt cctttaacgg atggatggtg ggatgtattg caagtttaag cttgcttcta
    17881 ggtggaggaa cagtatttgc ttttaatcat atcaatcaat cttcacaggc tgattctgta
    17941 gccacaacgg cgtcaatcaa ggaagaagcc caggaacaag caagaaaaga gtatgaacaa
    18001 caattagaag aattattgaa tcgtaccaat cagtcaacaa cagagtcaag catggaaaat
    18061 agcaaagaga gtgattctag taaggctaaa gaaggatcaa gcgattcaac tgtacaatca
    18121 aacacttccg aaagttcaga atcaaatgag gtgaaataat gcgtgttaga gaaacaacga
    18181 agcgaaaaga caaacgattc aatttagaaa agaataggta tctttctcat ttctatcgct
    18241 atactccctc aggagaagag ctaccagaaa aacatgacaa acgcatttcg aaaaagattt
    18301 tttatagtgg acttgtatac ctccctttag agtatttagt aacaagagta ccagaagttt
    18361 tcaaccgatt ggaaattgtt aagggaagct tacaaggaat aaatctgttt caattacagg
    18421 aaatgccaca ggtctttcat tttgattcag ctggtttttg gaatccattt actcctacaa
    18481 ctagtttggg tacacgttta gcaatttcga cagtttgtgt ggttccagct gtacttatgg
    18541 gattgaaaat ggacatgtgg tttcgtcctc tagcagatgg acaaaaaggg gataatcgat
    18601 taatgacctt aaaagaaatc caacagattt atccgcaaat acctgaaaca aaaactactt
    18661 ttccaggtta tggaggtatt ccaatcacac attacaaaaa atattggtac attcaaacag
    18721 atactgttaa tacgtgtatt gttggtacct ctcgatcagg gaaaggtcag attgaagtgt
    18781 tagccaccat tgacaattta tctcgtgcag aaaaacaaag ttcaatggtc gttaatgatc
    18841 caaaaacaga attatatgtt ggtagtaaag atgaactaga agctagaggg tatgatgtat
    18901 atgcctttaa tatcaccgat ccactgcaat caatgagtga taatccattg gcactcatcg
    18961 tgaaatactg gaaacgagga gacatagata ctgcaacaca gttaaccaat actttcacaa
    19021 atacgattta ttatgatgcc aacgcttcag ataacaaata ctttaacgaa aacgcgcaga
    19081 aagctgtcaa tgcgcttatt tttgcgttgt tagaacaagc tgatcaaaca ggagattact
    19141 ccaaattgac accgaataat ctcgttgagc ttctttctga gattggcggt tttaattatt
    19201 atccagatcc agacaatgaa ttcaaacagt tgaatgcctt agatgaattt atgaatcagt
    19261 tgccacctgg aaacattgca aaaaaacaat atggggcaac gaaaattgga tctgataaag
    19321 cgaagggaaa tattttatca acggcaatca cgggattgaa tccatttact ctaacgaaaa
    19381 ttgcaaagat gacttctcaa tcaacgattg attataaatc ggttgggttt ccaaaatact
    19441 tagatttgaa attacatgaa agtcttttaa atcaacgagt cgtagtagaa ttttatcata
    19501 agggaaagaa atttcatgaa gaacgtgtca aagtaggcta taaaggattt agtgaaatca
    19561 attttgattg tcatttagtt gcaggtgatc aagtttgttt aaaatacgat catcaaggaa
    19621 aagaatatgt ggcttggtat gaattttctc aaagagtatt gaaagacgaa gagggtaata
    19681 tcgtttatcg gaaaaaacga ggagaaaccc atttgccaga attagaaaaa gaagccgttc
    19741 taacattaga tgaggaaaaa agtaacttat tagtagagtc tattcggatg cactataccg
    19801 ataaaccaat ggctattttt atgcttacac ctgattatga tccatctaac catatcattg
    19861 ttagtatttt tcttagtcag ttatataaag aattatctac gcaatgtgtt aagactaaag
    19921 gagataaatg tcaccgcagg attcatttta ttttagatga atttgggaat agtgtgacct
    19981 gtttagccta aggataaaag aggctagttc caacgaacat tggaataact ttactatcga
    20041 gaaatcaaat gacggggtaa cgccctgaag ggtttccact gatactgcga catgcgttag
    20101 taggatgtgc atccaaacgc gtatgaagct cgctgaagtc ggcagaaggg taccgtaagg
    20161 aaagacacga tacgtatcta ccacgcaagt gctctagagg tagggtatgt acctgatatg
    20221 tcgggtgtcc taaaaagttc aataaggtga gaatgatttg attatctgac aaacttgcga
    20281 atggaatgtc tgaaacgaat aactcataga aatgtgaggg ggaaggggga tacgtcctct
    20341 ttccattgac tcgaggttga gtaaaaatgt gtttctatga aaaaccttgc atggttaaag
    20401 gctatggagt agtcaacagg caaaagcaag cacctaagtt gaaatctatg gatagatagt
    20461 aaggaacagg ggaagcatct agcacggagg gagcataccg aagatgtgtt tctaaggaaa
    20521 aaccgaaatc ggttataact taggtgaata gatgtgaaag tagagccacg accagtgaaa
    20581 atttcgtaat gaaattggag gaacaggctc aagtcgttat ttataatata agaaacctat
    20641 ttaaaatcat ggattctcgt aagactaaaa aggtcacgaa ccagaatgga gcgtgataaa
    20701 accatgacta acacaaaagg aattagctta aagaacaaac aactcagaca cgccgaatac
    20761 tacgacatga ctgagacttt cgatgaattg cacgcaatga gtgttgcaaa atcaaatttt
    20821 aaaaatctca tgaaattcat tattgatgac aataatatct ttctagcgta tagaaatatc
    20881 aaaaagaata aaggaagtgc aacggccgga tacgatggtg tcactataaa agacattgaa
    20941 aaattggcat gtggtgactt tttaactatt attaaaaggc gatttaagga ttacagacca
    21001 agaaaagtta aaagagtaga gatcccgaaa ccaaacggaa aacttcgacc tttaggaatt
    21061 ccttcaatat gggacaggat agtccagcaa tgtattctac aaattttaga accaatatgc
    21121 gaagcaaaat ttaataagca tagctatgga ttcagaccaa attgttctac agaaaacgcc
    21181 atagcagatt gtcttgctag aatcaatcaa agtaagatga attatgttgt agatattgat
    21241 ataaaaggat tctttgacga agtaaaccat accaaactta tgcgtcaaat atggacaatg
    21301 gggattcgag ataaacaact ccttgtaatt attcgtaaga tgttaaaggc tccgattgtt
    21361 atgcgaaatg gcaatgttgt ttttccaacc aagggtactc cacaaggagg gattttaagt
    21421 ccactgcttg cgaatatatg cctgaatgaa tttgattggt ggatagctaa tcaatgggag
    21481 gaacgaactg tcaaagagct gaaacaccac tatcgaaaaa acggagcatg ggataaagga
    21541 aatatctatt cgaaattaag aagccacaca agtttaaaag agatgtatct ggtacgctat
    21601 gcagatgatt ttaaaatttt ctgccgaaca ggagaagaag cccaaagggc tttttatgcc
    21661 acacaaaaat ggctcagaga aagattaaag ctcccaattt caatggagaa atctaaagtt
    21721 acgaacctaa ataaagctga aagtgaattc ttgggttttt caataagact ccacaaaaag
    21781 ggaaaaaaga aagtggcttt cacgtacgtt tgtcgcaaag cagaagaaaa cattaaaaat
    21841 aaactaaaac aacagattaa aaaaattcaa tacgctggag caaaaaaaaa taggcaccga
    21901 gcaataggtg actacaacat gatggtcatc ggtatccaca attactatcg gatagcttca
    21961 cattgtagtg tcaatttcac tgagatttat agaagtgtta ggacagtaat agaagcaaga
    22021 ctgaaagcta gaggaatgac gaaaaatgga atctatggac aagatcatga aggataccgt
    22081 ccgtatctaa agtctaagca aatgagatat attgaaggtt ttccgatact tccaattggc
    22141 tatattaaaa aaagaactcc actcaacaag cggagaaatc taaataaata taccaaggaa
    22201 gggagaaagc tcattcatca agagcaacaa gctatagaaa gctggaaaat caagtatctt
    22261 gtaaaaggtt ttagtaacaa taagaaatac acggtggaat tttctgataa caaaatatct
    22321 aagtttattt cccaaaaagg gaaatgcgct atcacaggaa acgaactttt gatggaagaa
    22381 attgaatgcc atcacattaa cccatatcat ttgtccaaag acgatagtta caagaacctt
    22441 gtcattatct ctagagaaat ccatagatta atccatgcaa ctagtgaaga aactattctg
    22501 aaactgcttc ggttactgaa actaaatcac tatcaaatta gaaaactaaa cacgtatcga
    22561 aaaaaagcag ctaattttcc aatatcaatc aaaatgaatt agataggaaa caaattatat
    22621 tgtaattaac gatggaacgc cgtgtgcggt gaaagtcgca cgcacggtgt ggaccggggg
    22681 aaaagacgga gataatatca aactcttacc tatcggaatt gccaccaatg gataatatgg
    22741 aaggaatcat gacggtcaca gctggccgaa acatgttgtg ggatctgttt attcaatctt
    22801 atcagcaact ttatgcgaag tatggagaac aaaacgcagc gattatcaaa gaaaattgtc
    22861 agacacatat ttatattatg tcgacagatg ataataccat cgaagaattt tctaaaaaag
    22921 tggggaataa gacggttgaa caagagaaca gtaccatcaa ttcattaggt atgaatactc
    22981 atatcaatcg caatgttgat tcagatcgta tcttaactcc tgaaagaatt tctacttttt
    23041 tagaaggtga aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa
    23101 tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata
    23161 ttgaagattt caatccatcg tgggatatca atgatataga catacattgt acacatgcgg
    23221 acttggatct taaggcttta caaattgatt atgattattt cctaccagat gaatatgcta
    23281 gaaatctcta tcatggactg catgatcaat tagaagaaaa aaacgaagaa aatatgtttt
    23341 ataaaatcgt tcaatctctt gagaatgaga cggctaaaaa attgttaatt caattttacg
    23401 aagaacaagc agcagatcga cttcaagaat atgttgaaaa gttgatcaaa aatgaagaaa
    23461 tttctgaaat gattgggaaa cggttgttat tggaatctga aaaggtggtg aaagaatgat
    23521 cattggagga attttttctg gcatagccga ctggtggtca ggcttattcg gagaagggga
    23581 tgcaaacccc aaaaccgtaa cgaaagtctt acaagattat ggtaattatc tgcagtatca
    23641 agatgtgttc agttggattt gtaatacttt tttatggggg ttgatcaaag ggttatataa
    23701 tctcaatact ttactagaaa aaaccattta tcaaagcttt gatttaaaag acttattgaa
    23761 cgccgctggg gtaaatgaac tatttcaaag tttgattagt aaggtattgg ctcttttttg
    23821 tgtcgtcaca ttaatttatt taggcttgaa attttttatg agtaaacacc caccaaaagt
    23881 caaaaatatc ttggttaatt tatttatggc catgatcttg atttttggtg gtagttcttt
    23941 aatcgatgaa gggttaaata tcagtaaaag cttttacgga gacgtcactg cagcaaataa
    24001 agcagataaa acgggttcac ctgcttttca aattattcaa aataatgtga tcgatgtcag
    24061 tacattacta gcaactgacc cgaataaagt agaatccctg ccaacagaca aaagaaatgc
    24121 tttaacagcg aaaaatttta aaacagcgaa tatcaatggg attattactc ctaaacaagc
    24181 taaagaaatg gcggataaag ataagagtgg tttatcagat ttacaaaagg atcggttaag
    24241 agcgttacaa taccgactgg aactggatga taatggaaaa gaaattcctg tgcaaatcag
    24301 tgatgaaggc ttagccaaat atgtttatac ttcagggtat cgtcgataca ttagtagtcc
    24361 agggattatt ttagctggtg aatcttcttt ggcggtggcc tatttattta tcctatttac
    24421 gatcgttact tgcattattg agctggtatt taaaaaattc tatttagtga ttgccgcgtc
    24481 aacggacttt gagacaggac aacggatgaa aagtgctatc gaagatattt ctcaatcctt
    24541 cttgttattg gcatttacaa ttttagaatt acgaatctat accttaatgc tttcaggtat
    24601 tggtgatttg catgcggcag gaaagatcaa tggattcttg tatgttgtcg ctttgattgt
    24661 actaaccatt gcgctattca aaggctctca atcagttacg aaaattttcg gcgttgatac
    24721 gagtctgaaa aacggcagta attctcttct tagcatgttt gccttaggaa atattgcgaa
    24781 aaatgtgggt agcggagcta aaaaccttgc aagtgctggt aagagtggtt taagccattt
    24841 aaatgatatt cgtaaaaatg gatttggcca tcgaaaagat gttacccctg ataacgggga
    24901 agaacaatca gacgataaag ctacaactgt acctaatagt aagaaaacag tttccgaaat
    24961 gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga
    25021 acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca ccttagaaga
    25081 taaattagac ggcacgagag ctaaagaatt ggctgaagct gtgaagaatc catccgaagc
    25141 actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg
    25201 tgaaggacaa gtcaatgcag cagtgaaagc taatacaaca accgaaaaag aaaatcaaga
    25261 aggtatagtg attccttcag tcaaagatca ccaagaagcg ttgcactcta atgaaaattc
    25321 gattgaagag aaaaattcca tagaagaaag aaattcagaa gaaggaaaaa atggacagtt
    25381 accaaaaact gatccagaac aatatcgcta taaacctaac agtgaccttg aaaatgagaa
    25441 gcgaatttca gatttatcaa aagtaaatca agaagacaaa gcagaaaatg agggacagtt
    25501 accaaaaact gatccagaac aatatcggta tagatctgtt aactccgaaa atgaaaaacg
    25561 catttcagat ttaccaaaac gaaatcaaga agtacaaacg gatgaggatt ccatcgttct
    25621 gccaaatgat aatggaatcg aagttggaac tgaaaaatca acgaaaattg gtttacctaa
    25681 ggcacaaaat ttaccaatga aagatagtcg tttgaatcaa ttcaaggata atgatcaaag
    25741 ttttgttcat caatcacctg caggtactgt tcaaatgaaa ccaagtcgtt tgcctgaaaa
    25801 tggaaatctg acaactgttt cttacaattt atctgaaagt ggtgagacag ggaatgcgtc
    25861 cgttcaaaat gttgtgacaa gagaagctgc accaaaacct attcaaaacg ttcatacagg
    25921 ttacaagcca gcaaatgtgg catttactgc accaacaaaa ataaatgtga caagagaaac
    25981 taaaaacagg aacaatgcat tgaaagaaca actattgagg gatatcaaac aggatatcaa
    26041 accataaaag gcctacaagt taggcctttt ctttatatga aaaggagaaa tcaattatgg
    26101 ataaatatat aaacatgaaa aaccctccaa gatttagtgg agaatggaag tttttcgctt
    26161 ttttcttttt agatctatta acgcttggtg gctgcttttt ttgggcgaaa atggtcaata
    26221 atcttgtcca cttaccactc ttttttgcac tcttgaatta tggcttatgg attgcgatag
    26281 cgttagtgat tgtttggcga ccctataata acccaggtca acgacaaatt tttgcgatta
    26341 tcgagtctat tttttttgcg gacaagaacc attatcatgc gttagatcct aaccgaaatt
    26401 atcatggtac gtataaggag gatgaataaa tgcgagtcaa aggacaaaat tcagttgaaa
    26461 ggaaatccat tttgcagttt ggaaaaccag aagagcgctc tatcttggac ttatttccgt
    26521 ataaatcctg tacagaagag ggagtcttaa ttactagaga agatcatttt caacgttatt
    26581 atcgtgtagt ttctacagac gtggaaggat taaatgaaca agagaaacta gaacgaatga
    26641 atcagttgac caccgtcatg cgtacgtatg tgccaacaat taagttagtt agtttgacca
    26701 cagagacgga tctaacggaa caaatcgtgc aaaagcggca gttattagat aaaaatcgta
    26761 tggaacagtc cttaggaaga aatctacagc gattaagaaa atatgaaaag aaattagtag
    26821 aggaaatcca ggaattgaaa cgagcagaac aggaacgccc tgatttaagc ttctttttcg
    26881 tcattgaagg aaagacaatc aaggatttgc aaaccagaaa ccgtcagtta atgcgatcta
    26941 gtggcatttt agggttaaaa ccactctaca aaaaagaatt gatcaagatt ctttatcgaa
    27001 tgaataatat gaatgatgag taggaatggg gaagagagaa tgaaatatcc gttgatatgg
    27061 ttagaaaaag atgttccgaa agaagagacc actagaaaat ttcctcacac tttaagcctg
    27121 ggtaagttgg gaaaaggaaa atcttttaac caagtaaaga atcaaacaag aagaggtgac
    27181 tgtacatgtt agaagcaatt aaaacagctt tttcaccaca aaatgatcaa aaggtacttg
    27241 atccattaaa gcaactagga aatcgggaaa tagaaatttt aaaatccaaa gggtatgatt
    27301 tagacttttt aaaacaagtg atgccacaag gtggattaaa tttcgatgaa gagtatgtga
    27361 cctatggaaa tggttattca cgatgtgtga cagtttatca ctacacaaaa aatcctacgc
    27421 ttttttggtt ggccaatctc atgaacaatc ctcatacagt ggcaacacta gatgttagca
    27481 cagatgaaaa gcaaaaagtg attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc
    27541 ggtcagataa tgaacgaaaa gcgactgacc gaaataaaag cgcggaagaa tacctagaac
    27601 tagcggaata tgcccgaaaa ttgacttcta atggggaaat ttctaaacaa gtcaaagtga
    27661 gagtacatgt ttacggaaac acgattcaag aagtagatga acgagcacag gaactgatcg
    27721 aaaatctcaa agggatggat tatcgtgcag tgacctatct gcacaaaacg aaaagagact
    27781 ggcaatcgat gttcaccatg ttcgatgaac aagctgaatg gtttggaaat atcaaagggc
    27841 aatcactacc atcgctaaac attggcggag gtttcccgtt ccaccaccaa acgctaaaag
    27901 atccaggtgg aacacaatat ggggaaacca tgacaggcgg cccgtttgtt tggaatgcca
    27961 cgtataaaaa tagtgtgcgg ttgtctacca ataccttgct tctaggaatg atgggagctg
    28021 gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt agctgcagga gactttattt
    28081 ggggctttga aaagggaaaa gactttattc cctttttaaa ggaatataac ggcattatgg
    28141 tgcgtttaga cggatctgac gggatgatca atcctttaga aatatttgcc acacgtacgt
    28201 atgatgaagc ttccagttta tatgacgatg gatcagtcaa agatttgaag atcaatgaag
    28261 cagcgtccta tcaaacgcac ttagacaaag tggtctatca ggtacagtta gtgagtcctc
    28321 agctaaaagg aacgatgaaa gcagaattta agacctattt aaatcgtttt tatgaagagt
    28381 atggaacagt accacgcggc tttacttcct caaattcgcg ttcaaatact gatacgcaag
    28441 tcacaggtaa agatccagaa gcttatccga cgttcaaaga atttttagac tttttaggac
    28501 aactggaatt accaggtgct tcccaagaga aaaagaatcg aaaagaagaa atggaatcca
    28561 tcgtagaatc cttatgtgag acgtatggca tgatttttga tggtcatagt acgattcgac
    28621 accttgacca acaacagttg gtcttttttg acattgactc tattacaggc ttagatcgag
    28681 gggtacaaca atgtcaaatg tacatggcta tgacgttgat ttggaatcag gcattgattc
    28741 aaggaagaag acaacgacgt ttaattaaag aaaaacaaat ggaagaacaa gacttcaaat
    28801 gcttcttggc gatgattgat gagtgccata atggtgcgac ctgttctgca gtgaaaagct
    28861 gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa gtcaaatgag
    28921 tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta gtttatgcag
    28981 accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata
    29041 gtgaatacca cgaaagttct ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa
    29101 aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg tactgtccga
    29161 aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat tgtgctaact
    29221 tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc gtaggagaag
    29281 ttagggggca aaagctagca tctaagttaa ggcatgtgga tagatataag ggaacagggg
    29341 aagccatcaa catggagagt gcatactcga tgaagtggta gtaagaaata tgtgaatgac
    29401 aaataactta ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg
    29461 aactggagga acaggctcga ttcgtctgta aaagtcatga aacccgttca accatgggat
    29521 tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa
    29581 caagtccaaa agataggaag ttgcggcatg cagaatatta tggcatgacg gaaatcttcg
    29641 ataagatata ctccaaaagt ttgaagggag ataatttcaa gaatctaatg cagattatca
    29701 tttctgataa caacattgtc ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga
    29761 caagaggaga agatggagta acgattaaag atattgaaaa tctcaaccgt aatgaattcg
    29821 tagaaattgt taggaagcga ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa
    29881 ttcctaaata taatggtaaa ttaagaccgt tgggtattcc atcaatatgg gatagaatcg
    29941 cgcagcaatg catcttacaa gtattagaac caatatgtga agcaaaattc aataatcaca
    30001 gctatggttt ccgcccaaat agatcagcag aacacgcaat agcagattgt atggcccgaa
    30061 tgaatcaaag tcacatggaa tatgtggtag atgtagatat caaaggtttc ttcgacgaag
    30121 tcaatcatac taaactcatg agacaaatct ggtctatggg tatacgagac aaacagcttc
    30181 tcttaatcat taggaaaatg ttaagagcac ccgtagtact tccaaatggg aagttgatat
    30241 acccaacaaa aggaacacca caaggcggaa tacttagccc attacttgcg aacatcaatc
    30301 tcaacgaatt tgactggtgg atagcaaatc agtgggagga aaaagatgcc aaagagctaa
    30361 aacatcattt taggaaaaat ggagcttggg ataaaggtaa tatctatcac aagctcagaa
    30421 agagtacaac tctaaaagaa atatacctag tgagatacgc agatgatttt aaaattttct
    30481 gtagaaatta taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc
    30541 gtctaaagtt accaatatct gaagaaaagt caaaaatcac aaatctttca aaagaagaaa
    30601 gtgaattttt aggctttacg atgaaattag tgaccaaagg taacaaaaag gttgttaata
    30661 cgcatatagg tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac
    30721 gaattcaaaa aagtcctaat agcaaagata cctttatcta tattggagtc tataacagta
    30781 tggttattgg gattcataat tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa
    30841 aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa ggaatctcaa
    30901 aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca ggacaaatac
    30961 gatatataag agggtttcca atattaccag tagggtatat tcaacacaga catccgatgt
    31021 gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt cataagaagc
    31081 aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg cttagtaaga
    31141 gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa aagggaagat
    31201 gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat attatccctt
    31261 atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag gaggttcatc
    31321 gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat ctaaaattaa
    31381 atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac gaagaaatat
    31441 cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt
    31501 atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat aatatcaaac
    31561 tcttacctat cagtatcgat taatccagat tttattgaaa cggtaaatta tgtcacaaac
    31621 tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa
    31681 gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa
    31741 ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga
    31801 ttagttggta aatcaatgac aaactcagaa ttagaatcca ttcctcaaca aaatcaagga
    31861 gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa
    31921 cgtcaattaa aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca
    31981 agcagtatgt taagcgtcgt gcaaagcgac gcttattttt gtggttgttt ggtacttctg
    32041 caggtttgat tactattttg attactgtgt tcgtgaccct ttttctaata cttgcggcgg
    32101 gttcgattga taattctgat tcagattcaa gtagtggtgg cgaagctttt acaggagaat
    32161 attcggaagg cttaccgatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga
    32221 ttgcgcaata tgctgtaggt gcagcagtga aatataaatt attaccaagt gtgatccttt
    32281 cgcaatacgg ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaact
    32341 attttgggat tacttggttt gatggttgtc tgtttcctaa aggaactgcg cgaggaatcg
    32401 gtggtattga aggtggctgg tacatgaagt ttcccaactc aaaagcagcc ttctcctatt
    32461 acggctttat ggtagccaca caatccaatt ttaatgcatg tgtaggaaat aaaagccctg
    32521 gcgcaagctt attgatctta ggacgaggtg gttatgccgc tgcaggaatt actgaagata
    32581 gtccgtatta tacaggttgt atgtccatta tcacttctaa taaattaacc gaatatgatg
    32641 aattcgccat caaacattgg ggtgaaggtg gcaataacaa cggcacgatc actggtgaat
    32701 ggacgaatcc attccctggc agcagtttag ataaaagttc cttcagtggt ggtcaattat
    32761 ttggaacaaa tccaggcggt gaattccgtc cgaatggctt ccatgatgga ttagactttg
    32821 gttcagtcga tcatccagga agtgaaattc acgcggtgca tggaggaaaa gtcgtgtacg
    32881 ttggcaatcc tggtatttca ggtttaggtg catgtgtgat cgtgattaat tacgacggtt
    32941 tgaatatggt atatcaagaa tttgcgaata gtacaggaaa ttctcgtgtg aaggttggcg
    33001 atcaagtgaa agttggtcaa gtaatcggga tacgagatac agcacacttg catttaggtt
    33061 tcactcgaat ggattggcgt caagcacaag gacacgcatt cacagacgat ggtacatgga
    33121 ttgatccatt accatctttg aatagcagca aaaaatagat caggagaaaa aatatggaaa
    33181 ataaaacttt aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatatcg
    33241 cgttgttttg gcagctgaaa gaagaacaag agacaaatac ctttaaaatt gaagaagtgg
    33301 ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca
    33361 agttaaatca aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg
    33421 gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc
    33481 aattggccat tgataaatca ttaagtagaa atgaattaac ggaaaaacta aaagatgtcg
    33541 caacccaaga agtgatcgac aaattaagtc ctgcagacga tgatcaccaa catgatgatt
    33601 acggaagtta ctcctttaca ttaggtgacg tgcaaactta tgctcaaaca agcagtgtaa
    33661 aagaggaaca aaattttgtc gtatttgttg attatacaat cggcaatccg caattcaaag
    33721 atgtcaaacc acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc
    33781 aatggaaagt catagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa
    33841 aaaggagaaa gacaacgttt caaaaaacga aaaagttatc ttcaatggct gggaatcttt
    33901 atgattctcg gctttttgat tcttgctagt ccgatttata cgaatcagaa ggtagatata
    33961 ccagatgagg taagcacgaa cgccttttta aacacgaaag agctgacaat ggtttcaaaa
    34021 gaaaaaggag cgaaccagca attagttttt gaatttgcga tcgatgatga agatcaaagc
    34081 ttgttaaaag aactggctaa tcttaattat cgcgtagaag taaagacagc taaaggggat
    34141 tatcaagcaa tacgagccaa agtgatcaag gtgtctgatg attattttgc agtgaagcta
    34201 acgcatgtac cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat
    34261 ccaaaagtgg atatgcaaga accacaagat ctgatttatt acgtgcacga agataaggtt
    34321 aaagatcaag tgaaagatga agattatgaa caacacgcga tcaattacaa agtgaaagga
    34381 tacaagaaag aacaaaaagc agctcaaaaa caaatggaat ctttccaagc aaccattgat
    34441 ttgaatgaag aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat
    34501 caggaaaata ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg
    34561 caactgaaag atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca
    34621 aaagaaaaga tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac
    34681 aaagatactt tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct
    34741 aaagaaatat gtcgagaagt gaatttatct aacacaacaa tatctgagtt gaagaatggt
    34801 cgaaagaatt tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa
    34861 aaatatttta cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat
    34921 aaaaaatgct tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa
    34981 cattaaccac gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc
    35041 tggctgaaga tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct
    35101 taaattcatt tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt
    35161 ttattgtcta aatacacaaa taaccttaat ttttaaagag cataaggaaa cgtgatatca
    35221 ctttctcacg aaaggtgtta ggtaggctgt gcctaccacg tttctaagaa agataaatga
    35281 tatcaaaatt ttggatttgt gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt
    35341 aaaataagcg tttgtgtgta gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca
    35401 tatccctaca cataataaat ttggaggaaa atatggactt attaattaga aatattgaag
    35461 aaaagtatat taataaaatt gacaaacgtt gtgaagaact tacggtcaag actggcaaaa
    35521 aatggagtcg aaatcaatac ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc
    35581 tgaattataa aaaagaccaa tttgatcggt tgttagaaaa atttgtggat attcaaactt
    35641 ataatacgaa aactttagaa gaatatattg caacaaataa tcaattaatt ggtttgttaa
    35701 ttgaataata aaaatagtat tgtgaattta taaaaggagg aaatcaattt tggcaaaagc
    35761 aacaagaaaa catgtatatc tttacgatat gcatatcgaa gtacttgatg aaataatttc
    35821 tactcatgaa gatatatcta attatagtga agcagtcaga catttgtgta tgatgtatga
    35881 tccaaaaatc gaagaaaaaa taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga
    35941 aatttctatt ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg
    36001 aatactaatt ggtacaaatt caactgttta tcaagaagca gaagaacgtg tggaaggaaa
    36061 aattaaatcc aatcaaacca aaaaatattc ttctaagtta aagaaaccag tgaatgaatc
    36121 tgttcatacc agagtagatc catttgatcc aacgaagatt ctaaaaagat aaaagtagaa
    36181 ggagaacgtg atgaataaat cgcctgcagt tattcttact tgtcaattta ccttgcccaa
    36241 taaaaaatct ttttcaacat acattgatta tatgacaaga gaaaaagcac tggaagaaat
    36301 ggaacgacgt acgcccgaag aagaacagga actgcaacga atcaagaatg ctttagagga
    36361 tttttatatt cctgaaggag aaactttttc ggaaagtaag acggataaag aattcagtga
    36421 aacgtcgaat gaagctcaat cgttgatgga acatggaatc aattttgaca agttagagga
    36481 acaagatttt accaaatatc tttcttatat gacccgtcac tatgcgttag aacaaaagaa
    36541 agaactgacg caaagggaac aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata
    36601 tcaaacagag ccacataaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc
    36661 agacgaagtg aaaggaagtg atctgaagga gatcaaacaa atcttccaaa gaggacaaca
    36721 aaaaggctca atcatttatc aagatgttat ctctcacgac aattcgtatc ttgaaaaaat
    36781 gggcttgtac gatcccaaaa cagatacctt agatgaagaa ggtttgaaag cagctggaag
    36841 aaaaatgatg gagacattat tcgagaaaga acaattgaat gaaacaggtt actggatggc
    36901 aacgattcac cgtaacacta agcatatcca tattcatttt gcagttgttg aaaaagaaaa
    36961 tacgcgtaaa ccgtatactg agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa
    37021 gagaagacaa gcaacattag acgcaatgaa aaccaattat gtacatgagt tagaacgttt
    37081 ctcttatgaa aaggatcgaa tgatcggtat ggagaaacga aatttattga cacgaaaaag
    37141 tgatttgcgt aacaccttaa caaaagtagt aaaagaacca gcacgttacg atcgacacgc
    37201 catgaactta ttaaatgaag tatataaaac cctacctgaa aaacgttcgg aatggaatta
    37261 tggagatgaa aatcgaacca agctttcacc atttacaagg ggaaaacttg atgagttaac
    37321 caaatatgtt ttaacaaaag aacctggata tcaagaatat atacagattt caaaagcatt
    37381 gaaagaagaa gccaaaagcc tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa
    37441 aaacgccctc tttgatttaa aaaagcgcac aggaaatgcg attttaacag aactgaagag
    37501 acaggatagt caaataaaac cattaataga aagtgtcaat cagatgcaat ctatttacaa
    37561 tgaacgatcc tttcgaacac tcaaacagtt gccaacaaat gaatacaagt atagttatca
    37621 agaattcgta aagcgtagga aacaattcca acaaagagtt gtcacgcagc gaagcttgcg
    37681 tcaattaaat cgaacaatta atgcacatga agaaaaatac cacgcaagta aagcatatga
    37741 agagcaacag cgaagaatcc aaatggaaca agaaagacaa taggaggaag agaaatgaaa
    37801 caatatttag atcaatggaa agtcatagaa ggatcactaa aagaagaacg aatcgaacaa
    37861 cttcctgatt gtttagaaaa agagcatttg tttcagataa gcgaaatgtt gagaaacgaa
    37921 cagtttgatc caaatcaatt tttagttgtt gagtacccag ctactggtgt gtattgttgt
    37981 aatcatgtga agggtgaaaa atattttatt attcaagaat acgaaggtaa gttagcgcct
    38041 tattatacta cttgggaaat aaatgaagag ggaatcaata atttcccatg tgaaagcatt
    38101 gaagagtcta tttctctcac ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg
    38161 tgattgtcca ggactctttt tcaatgaatt gttttccgcc aattcactga gtggtgcgat
    38221 tcaattttat tatatcaaaa gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga
    38281 ctcagacaac gattggtgtt gtggttgaaa aaagcaggga taatctcgtt tttgtgacag
    38341 aaattcaaac aggtagagct tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg
    38401 gcaatatttt aacgttaaat ctgactacga agatgttcgt tgatgcagta gaaaattatc
    38461 catttgttta gacaaacgat attttacaag gaggaaacgg acatggcaga agaaaaagaa
    38521 ccactagata atagtcgttt gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa
    38581 ttaaataatc aatttcaaaa gctatttgat caccaaaaat tgacaaatgg gcaaccaatg
    38641 aatgacaaac gaaatggtcg ttcatggttt aaccagcaag aaaaaataga aaataaaata
    38701 caaagtttga gagaagagat caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa
    38761 gaagagctaa aggaaatggg ttataacaag tacggtggcc tggacatgac gatcgaaaat
    38821 attccaagaa tcaaagaaga aatcgaacgc tttgaaaaag gtgaatcgac tttttcagct
    38881 gccacgatcc gaaaatacca acgaaaattg gaaacattag aacagctgaa agaaaaatca
    38941 gagaaaggaa aagaaaatct tcttcctgaa gtacaagcga tcattgacag cggacgagtg
    39001 acgcaatgga aaaagaatcc aactatctat tttctgaaag gctatcgaaa agtcgcttta
    39061 gaactcaatg aaaacggggg atttgaggaa tctaaaaaat accaagccac gtcggaagaa
    39121 gaaaaggcag ctgttcgtga attacttgaa gaagctacac ctactgattc tgtgaaggag
    39181 aaagaaaccg atcatttcta tcgagtagaa ttcaacgaaa atgatccagc aaaagggttg
    39241 aaaagttatg gtggggagat cgtaacacta gaattgatcg atcaactcaa aacactggat
    39301 aaccaatacg ccgcagaagc aggctattat aaattctatt ttgaagaagt gaagaatgga
    39361 gaagtagtcg atcagatacg aatggatcta ggggatggcg tcaatagtaa tcaccccatc
    39421 tatttagagt tagctagatt gtgcgaacaa tcacctgaaa aggaacaaga acaaacagtc
    39481 aaaaatgaac ctatttcaaa agtacaaaac gagattaaag agacaaaaac aaaacaaaag
    39541 ggttatcaaa aacgaacggc agaagagatt aagcaagaag tcaaatcact ttcccaacag
    39601 gcgcttgatg cggtgaaaaa gcatactcat tcacctgaag acgtcaaaga acttttgaat
    39661 tttatgtctc gttttccaga acgatccttt agaaatcaat tattgatcga ggaacaattt
    39721 ccaggcgcaa cggcttgttt aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa
    39781 aaaggagaac aagggaacaa gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa
    39841 ggacgtggct ttgttcgaga aacagaggct acaccagaag ataaaaaacg aatcgaaagt
    39901 ggtcaaatcg aagtgatcaa aaaaccttac tacacgattg aaaaagtgtt tgatatcaca
    39961 caaacacaat tgaaacctga agactatccg aaaatcttcc cgaaccgcgt ctttgatttt
    40021 caattggatc aaacagggca gacagaactt caagttggga ttcaagcggt agccaaaaat
    40081 attggaattc aaattcgaga tatgacagaa agtgaagtct atcaaagaga attaggacaa
    40141 gcacgtgggg cgtacgttcg taacgaattt actggcgaag aagaaatcgt catgaatact
    40201 cgaaatactc ctacgcagca tttagccaca agtattcacg aattagccca tgctagaatg
    40261 cacaaatttt cagaattaga tactgcaaca aaagaactac aggcagaaat gacctcctat
    40321 attgtgtgca agcattttgg gatggacacc tcggagaaag ctattcctta tattgcgaca
    40381 tggacgaaga atagtcagac gttggatgat aaggaagctg cggaacgtgg gaaaatcatg
    40441 aatgatgtca gtcgtgtcgc aaatgaattc attcaaacca tttctaatga aatcaatcaa
    40501 tacagagaaa gagaaccaga gatccagcca gcagaagtga agcaggagca taaaaatact
    40561 gtagaaacgg cagaacaaaa gaatattgtg cccgttggct cactttggat cgacaagaaa
    40621 gacgggaaag tcctggaaga agacacgggc cgaacgttac atttgaagga tgcggcgttt
    40681 attgaagatc cgcagcataa tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatc
    40741 caaacggcgt taccttcaga agaatttgcg ctaggaaatc cattccgatt ttcacttgag
    40801 acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa
    40861 aaagaacgcg tataaaacaa gcaagaaaga ctatctgaaa aatggatagt cttttttctt
    40921 ttgggaggtt tgaaaatgac agagaaaaaa atgagtctca ttgatcgatg caagcaaatg
    40981 gatattgtgg attttgcacg aaacaatggt ttagcggtgg ccaataaagg acgagactat
    41041 cgcttagaag accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac
    41101 agtcaaaata ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag
    41161 atccaagacc ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac
    41221 aaaacagaac gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt
    41281 gattatcaac cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactgccc
    41341 gattggttga ttgattatgc agaaaaagaa ggattgattg ccgaactaaa gccaaaacat
    41401 gaacggcaaa atttcttagt tcgagacgat cgtttggatc atgcggtcgc ctttttatgg
    41461 aaagatccac agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat
    41521 gaacgttttg gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat
    41581 ggctttaacc taaagattgg tgatcctaaa cagttaaaat tttttgagag tagtattgat
    41641 ttacttagct acgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg
    41701 gaagggttaa aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga
    41761 aagaaacaag cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat
    41821 attttctacg aaaaagaaca attaatgggc gcagttgatc catttacgaa tcagaaagta
    41881 cgttgtgaac gaggaatcgc taatgattgg caagtgccaa gagaatacaa agtcatttat
    41941 gaagaagtcg caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca
    42001 gaaaacaatc tgcaattaac gaaccaactg gtttccgctc acaaagtcaa tgcgtctttt
    42061 ggtcagcagc tttctgttaa cgattcaata gaagtcatta gtttgaaaga tatttgtaga
    42121 gaagtcgcaa aagaattaaa agattgtgaa cgctcgaatg gtacgtatga ttttgatcgt
    42181 ttttatcaag gaaaagggga tattaattct cagattcttt tctcttataa agcagaacaa
    42241 tactacaaag gctataaaaa tcatgaacat gaatttgtac ctgaagtgaa aaaggattgg
    42301 aacgatcaat tgaagcatga gatccatcaa caggaaatta gaaagcaaaa acgtgcgatg
    42361 ttgtttcaac aaggcagaca acaagaaagg gagtgagatc gtcacgatga aaactgtcat
    42421 tttagcagaa aaaccgtcac aagcgaaggc ctatgccgat agcttttcta aagccacccg
    42481 aaaagacggg tattttgaaa tccaagatcg attgttttcg ggtgaaactg tgatcacgta
    42541 tggttttggt catttagtcg agttagattc gcccgatatg tacgatgaaa attggaaaca
    42601 atggtcatta gagcatttgc ctattttccc tacacattat cactatcatg ttcctaaaga
    42661 taagaaaaag caattcaacg tggtgaaaca gcaacttcaa tcggccgaca ccattattat
    42721 tgcgacagat agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagcagg
    42781 cgctgatcaa ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat
    42841 ttatcaggga ttccagcaat tacgagacgc agaagagacc tatcccaaat ttgaagaagc
    42901 acaagcacgc cagatcgctg attggttgat cggcatgaac ggcagcccac tgtatagttt
    42961 actcttgcag caaaagggca taccaggaag tttttcctta ggcagagtcc aaaccccaac
    43021 gctttacatg atttatcagc tgcaggaaaa gattagaaac ttccaaaaag agccttattt
    43081 tgaagggaaa gcccaagtaa ccgctcaaaa tggcatattt gatgcgaagc ttgatcccaa
    43141 tgaaacccaa gcgacgcaag aagcatttga agcctattta aaagaaaaag gtgttcaagt
    43201 aggaaagcag ccaggcacga ttcttgaagt cgaaaccgag aaaaaaagcg cggcaagtcc
    43261 acgtcttttt tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc
    43321 caaagatact ttggaagcca tgcaagggtt gtatgaaggg aaatatctaa gctatccacg
    43381 aacagacacc ccttatatta cagaaggaga gcatgcgtat ctgttggatc atttagatga
    43441 atacaagcat tttttaaagg ctgaatcatt ccctactcct attcatacac cgaatagtcg
    43501 gtatgtgaat aataagaaag tccaggaaca ttacgcgatc attcctacaa aaacagttat
    43561 gacagaagca acgtatgaaa agctgacacc tttacaacaa gcgatttatg atcaagtttt
    43621 aaagacgacg gtggccatgt ttgcggaaaa atatacctat gaagaaacta cgattttgac
    43681 acaagtccag cagcttcagc taaaggcagt cggaaaagta ccagtagatc taggttggaa
    43741 aaagctattt ggcaaagaga gcgacagaaa agagaaggaa gaagaaccgc tacttcctaa
    43801 agtaacaaaa ggagaaacgg tgacggtaga tctacaaatc tttgaaaaag aaaccaagcc
    43861 accacaacct tacacggaag gtacactaat caccgccatg aagacagctg gtaaaaccgt
    43921 ggatagtgaa gaagcacaat ccatattgaa agaagtggag ggaattggta cagaagcgac
    43981 acgagcaaat attattgaaa ccttgaagca aaaggaatat atcaaggtag agaaaaataa
    44041 acttgtggtg acgaataaag gaattttgct ttgtcaggct gttgaaaaag aaccgctgtt
    44101 aacaagtgcc gaaatgaccg ccaaatggga aagctatctt acaaagattg gtgagcaaaa
    44161 agggacacaa gctacttttc tagcaaatat ccaaaaattt gtttcccact tgttggaagt
    44221 cgtcccagga caaatccaag ccactgattt tggagcaacg ttacaagagg tgaaagtagc
    44281 cagtgaaaag caagaagcgg cgcgtcattt aggatcttgt ccaaagtgcc aggaaaaaga
    44341 agtcatactt tatcagaagg tggcgacttg cacgtctgaa gcctgtgatt ttaaattgtg
    44401 ggtaacgatt gccaaaaaga aactcacagc gacacaactg aaagagatca ttcagaacgg
    44461 ccgaacaagt caaccagtca gaggactaaa aggtcagaaa ggttcgtttg aagcaacgat
    44521 cgttttaaaa gaagacttta caacaggatt tgagttttct gaaaagaaga agaccaccta
    44581 caaaagaaaa gtgagacgta aaacgagtta gaattgacgg agggaaaaac gatgagaaat
    44641 acaacgcata aagaacaaga aatcgaaaat caaaaagcca aaatacgttc agagcaggag
    44701 aaaatgcgtg ccagcaaacg aagactacag caattgaaga atcaacaacg gattcaaaaa
    44761 gaacttgaat tagatcgata aatcctcctg ttgttaatat taaactaaca tgttatagtt
    44821 atgttagttt aatattaaca ttcgagaggg aaagcgatga aagataaagt caagtgcatt
    44881 tttgagaaat acaatggtaa tctatccttg aaggatgcta gaaatgaagg gttatcacct
    44941 atgacgttag atcgtttagc cgaaaaggac aaaatagaaa aaatagcatc aggtttttat
    45001 accttaaaag atagttttgt ggatgaatta tatctcgctc aaagtatcta tactagagga
    45061 atcttctctc acgagacagc gcttgatctt tttcaagtag gaacatatat ccctaaaaaa
    45121 attaatatga tgtttcctaa aggatacaat gtttcgaaag agaatttaga tcagtatgcc
    45181 attcaaccgc attacacgaa taaagaaaac tttgaactgg gaatcacaga ggcagaaagc
    45241 ttttacggca ataaaatttt cgtctatgat ttagagcgaa cgttatgtga tatgtggaat
    45301 cccagatata aagcaagcgt agaagtaaaa caagaagcat taaaagaata tatgacaagt
    45361 accagcagaa atacaaataa gctaagaaga tacatgaata ttttaaaatc accaaaagaa
    45421 atgactttgt atatgttgcc actttattga gaggagtaaa ggtgaatgac accgacacaa
    45481 tttaaagcga aaacaagaaa tgttgcagct gagaaacatt taaacccaca aattgttcaa
    45541 agatattata tgatggaaaa attattagaa tgtattgttg aatcgcctta tcatgatgat
    45601 tttgtattaa aagggggatt tctgatcgga tcaaaatacg ggttagaaaa ccgttcaacc
    45661 gttgatattg atacgactta tcgaaattcg aaactaacaa aagaaaaatt agaaactgtt
    45721 ttttctgaat taacagctgc tcctacaaaa gatggcattc ggtttgaatt aaagggctta
    45781 acagagacga gggaagccga ttattaccca ggttttcaag caaagtttat tgcttttctt
    45841 gaaaatgcac gtgtaccttt taaattagat attacttcag gtgattctat tattccagca
    45901 gcattgatat atgaacataa attgatgttt gaagataaaa caattcatat tccagcttat
    45961 cctacggaac aaattttagc tgaaaaatta catgccacgt ttagtttcgg aatagataat
    46021 tcacgtatga aagatttcta tgatctgtat atgatcccta aactagaacg tatcgatgag
    46081 ataaatttgt ataagagtgt tcaaggaaca tttaaggaaa gaaataatca atcagtgttt
    46141 aagaatttgt ataatcagga aatgcctcaa ttgatgaaga atgagggatt gagggagaaa
    46201 tggaaaagat atcagcaaga aaactatttt gccaaagata tttctttcga atctacctta
    46261 acttctgttg attcgctgat gcaatcaatt gtcgagcaag aggaagtaga acgaaaacaa
    46321 aattttaaga gatcacaaca aatgaatcaa gaaatggaga gataaagatg atcaataatg
    46381 taactttagt aggtcgatta accaaagatc ccgatttacg ttacaccgca agtggcactg
    46441 cagtgaccac ttttacctta gccgttaacc gtaattttac taatcaaagt ggcaatcgag
    46501 aagctgactt tatcaattgt gtgatttggc gcaaaccagc tgaaacgata gcgacattag
    46561 ctaaaaaagg cagcttgatc ggcgtcgtcg gacggattca aacgcggact tatgacaacc
    46621 agcaaggaca gcgtgtctat gtgactgaag ttgtggccga taatttccaa ctattagaaa
    46681 gtaaagcagc gacagaaagc cgtatgcacg ctgatcaatc gtccagcagt ccaagcccta
    46741 ccacctttga acaacgggat acagcgacgc ctaacaacaa tggattgaac gcttcacaga
    46801 atccatttgg tggacaatca atcgatatta gcgatgatga tctaccgttc taagtagcat
    46861 tttctattgg ttgcatttgc tacggtttat gttataattt agacaacaaa aagaattttc
    46921 aatctataac caaaaaccac tagctggcgg gctagtggct ttcttttacg tttctattat
    46981 aagaattaac gggtaggctt actgtctttc ttggcaaatt tacaatcaag ccatttgcag
    47041 atatagtgcg cggctacttc tgccatgatt gcaaaaacaa aatcaaaaag aattttcaaa
    47101 cctataacct cctccccttg ccagattagg ccggggtagc agacagcaag actattatag
    47161 cataattgaa atatccaaat gattggattt ttgctataat tttagcgagg tgattgtgat
    47221 gaacaaatat gttggtttgc ttgataaaat ccgagtaata aaaacgcaac cgttattggt
    47281 tcgctttact ttacaaacga ttcatgaatc gattaattgt gtagttgcag atattgaaat
    47341 tatcgataaa ttgctaatta tggatgatgg aaaatacaat attgccgtca caggtcactt
    47401 caataaacga aatcagctag ttattgctag catgtatgtt agaaatcccg atcattttac
    47461 gcgatcgatg ggcatataaa taaaagaaga gttgtagtcc taaatgagga ttgcagctct
    47521 ttttgtttta catagaaaca ggaaaagacc tttttaggaa gacacaagtg ccttctcaat
    47581 tgaaaaacct ttctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgc
    47641 tgcacgttca ctaagatatg gtgtcttgca aagcaagaca aaatagacaa aacttcctct
    47701 caccaaaagg ttc
//
LOCUS       DACDCV010000010        47464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDCV010000010 DACDCV010000000
VERSION     DACDCV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.010"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV041_000599"
     CDS             complement(38..1189)
                     /locus_tag="IV041_000599"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8381417.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV041_000600"
     CDS             complement(1202..1639)
                     /locus_tag="IV041_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8381418.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV041_000601"
     CDS             complement(1654..3420)
                     /locus_tag="IV041_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8381419.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV041_000602"
     CDS             3684..4361
                     /locus_tag="IV041_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381420.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4528..5490
                     /locus_tag="IV041_000603"
     CDS             4528..5490
                     /locus_tag="IV041_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381421.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IV041_000604"
     CDS             complement(5690..6130)
                     /locus_tag="IV041_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8381422.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IV041_000605"
     CDS             complement(6141..8174)
                     /locus_tag="IV041_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8381423.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IV041_000606"
     CDS             complement(8176..9879)
                     /locus_tag="IV041_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381424.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV041_000607"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV041_000607"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8381425.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IV041_000608"
     CDS             complement(10962..11987)
                     /locus_tag="IV041_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8381426.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IV041_000609"
     CDS             complement(12196..13065)
                     /locus_tag="IV041_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8381427.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IV041_000610"
     CDS             complement(13524..15416)
                     /locus_tag="IV041_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381428.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IV041_000611"
     CDS             complement(15413..17140)
                     /locus_tag="IV041_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381429.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IV041_000612"
     CDS             complement(17156..17806)
                     /locus_tag="IV041_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8381430.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IV041_000613"
     CDS             18010..18306
                     /locus_tag="IV041_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8381431.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IV041_000614"
     CDS             18478..19098
                     /locus_tag="IV041_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8381432.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IV041_000615"
     CDS             complement(19190..20356)
                     /locus_tag="IV041_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8381433.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IV041_000616"
     CDS             complement(20368..20721)
                     /locus_tag="IV041_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8381434.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IV041_000617"
     CDS             20849..21871
                     /locus_tag="IV041_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8381435.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IV041_000618"
     CDS             complement(22310..22744)
                     /locus_tag="IV041_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381436.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IV041_000619"
     CDS             complement(22854..24713)
                     /locus_tag="IV041_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8381437.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IV041_000620"
     CDS             complement(24831..25991)
                     /locus_tag="IV041_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8381438.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV041_000621"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV041_000621"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8381439.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IV041_000622"
     CDS             complement(27738..28436)
                     /locus_tag="IV041_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8381440.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IV041_000623"
     CDS             complement(28393..30054)
                     /locus_tag="IV041_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8381441.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IV041_000624"
     CDS             30272..31672
                     /locus_tag="IV041_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8381442.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IV041_000625"
     CDS             complement(31924..33003)
                     /locus_tag="IV041_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8381443.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IV041_000626"
     CDS             complement(33089..34048)
                     /locus_tag="IV041_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8381444.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IV041_000627"
     CDS             complement(34060..35583)
                     /locus_tag="IV041_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8381445.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IV041_000628"
     CDS             complement(35593..36156)
                     /locus_tag="IV041_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8381446.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IV041_000629"
     CDS             complement(36162..37025)
                     /locus_tag="IV041_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8381447.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IV041_000630"
     CDS             complement(37039..37935)
                     /locus_tag="IV041_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381448.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IV041_000631"
     CDS             complement(38110..39381)
                     /locus_tag="IV041_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8381449.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IV041_000632"
     CDS             complement(39371..40888)
                     /locus_tag="IV041_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8381450.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IV041_000633"
     CDS             41086..41976
                     /locus_tag="IV041_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8381451.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IV041_000634"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IV041_000634"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8381452.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IV041_000635"
     CDS             complement(42885..44357)
                     /locus_tag="IV041_000635"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8381453.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IV041_000636"
     CDS             complement(44994..45356)
                     /locus_tag="IV041_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8381454.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..45944
                     /locus_tag="IV041_000637"
                     /pseudo
     CDS             45579..45944
                     /locus_tag="IV041_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(46106..47254)
                     /gene="tnpB"
                     /locus_tag="IV041_000638"
     CDS             complement(46106..47254)
                     /gene="tnpB"
                     /locus_tag="IV041_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8381455.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(47271..>47464)
                     /locus_tag="IV041_000639"
     CDS             complement(47271..>47464)
                     /locus_tag="IV041_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8381456.1"
                     /translation="ELTKFINAYKSASSRLIKRDFPRVKQFLWKEMFWSKSFCLLTTG
                     GAPIDVIKKYIQNQGNNHK"
BASE COUNT        13753 a         9833 c         8194 g        15684 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtgtgaa ctactcacca
    45961 cttagctaaa cctgacggtt cttaacgctt gaagtgggag cttcttggga atagagcata
    46021 cttgatagcg tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag
    46081 ttcctacggt tcttgatgat tggatctaag ccaatcccat agaatgtagt cttagacctt
    46141 cggtcagaat attgatacta gcattgatat cacgatcatg atgagtatga caaacaggac
    46201 aagtccattc tcgaatttca agagatttct tgccatcttt atgtccgcac tctgaacaaa
    46261 tctgactaga cggaaaccat ttatctactt tgatgatttt tcgtccatac cagtcagcct
    46321 tgtattgtaa tttagtcaca aactttgacc aagaaacatc agaaatactt ttagctaatt
    46381 tatgatttcg caacatacct tttgtgttta agtcttcaat acagataata tcgtgatttt
    46441 tgatcatttc tgtactcaac ttattcagaa aatcagtacg ttgattcatt actttttcat
    46501 gcaatctagc tactttgcgt ttttgttttt gataattttt agcttcaaat agattggtac
    46561 ctttattttt agccaataaa gcacgcctag acaatttgcg ttgttcacgc tttagtttct
    46621 tttccatttt ggacgtgaat ttattattat caattttttg tccatcagaa agaatcgcaa
    46681 agtccataat acctaagtca ataccaattg tggaattagt tttaggtaat tcgataattt
    46741 cttctttaca caacaaagaa atatagtatt taccgctaga atgacgtgat attgtagcgg
    46801 atttgataat cccttttggt tgtctatgaa gcttaattct tactaatgac ttcaatttag
    46861 gaactttgat gaatttacta tcaatcaaag caactgtacc attttgatta ttcgttgtat
    46921 aactctgaac aggatttttc ttacttttga aacgtggaaa tccaacggat ttatcccgaa
    46981 aaaaattctt gtatgcttta tctaaattaa gttgggcatt ggctaaagca aggctatcaa
    47041 tttctttcaa aaacggaaat tctttcttgt atttagctgg tgtcggaaaa gtcatttttt
    47101 tagaagaatc atttttaact tcttcatacg ccttttttcg gtcatcaagc attagattgt
    47161 agaccttacg gacacaacca aaagatttgg caaagaagat ttcttgttcc tctgttggat
    47221 agattctgaa tttgtatgct tttagttgtt ccataaagtt cacctacttt ctatttatga
    47281 ttgtttcctt gattttggat atacttctta atcacatcaa ttggtgcgcc accagttgtc
    47341 agaagacaaa aacttttaga ccagaacatt tctttccaaa gaaattgttt gactcttgga
    47401 aagtcacgtt ttatcaatct tgagctggca cttttatagg cattgatgaa ttttgtcaat
    47461 tctg
//
LOCUS       DACDCV010000011        44588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDCV010000011 DACDCV010000000
VERSION     DACDCV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.011"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            146..793
                     /locus_tag="IV041_000640"
     CDS             146..793
                     /locus_tag="IV041_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8381457.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            808..2334
                     /locus_tag="IV041_000641"
     CDS             808..2334
                     /locus_tag="IV041_000641"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8381458.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2383..5181)
                     /gene="ileS"
                     /locus_tag="IV041_000642"
     CDS             complement(2383..5181)
                     /gene="ileS"
                     /locus_tag="IV041_000642"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8381459.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5443..6150)
                     /locus_tag="IV041_000643"
     CDS             complement(5443..6150)
                     /locus_tag="IV041_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8381460.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6171..6953)
                     /locus_tag="IV041_000644"
     CDS             complement(6171..6953)
                     /locus_tag="IV041_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8381461.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7002..7289)
                     /locus_tag="IV041_000645"
     CDS             complement(7002..7289)
                     /locus_tag="IV041_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8381462.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7304..7903)
                     /locus_tag="IV041_000646"
     CDS             complement(7304..7903)
                     /locus_tag="IV041_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8381463.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7917..8594)
                     /locus_tag="IV041_000647"
     CDS             complement(7917..8594)
                     /locus_tag="IV041_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8381464.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8611..9852)
                     /gene="ftsZ"
                     /locus_tag="IV041_000648"
     CDS             complement(8611..9852)
                     /gene="ftsZ"
                     /locus_tag="IV041_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8381465.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9875..11200)
                     /gene="ftsA"
                     /locus_tag="IV041_000649"
     CDS             complement(9875..11200)
                     /gene="ftsA"
                     /locus_tag="IV041_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8381466.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11356..12576)
                     /locus_tag="IV041_000650"
     CDS             complement(11356..12576)
                     /locus_tag="IV041_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8381467.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12591..13679)
                     /gene="murG"
                     /locus_tag="IV041_000651"
     CDS             complement(12591..13679)
                     /gene="murG"
                     /locus_tag="IV041_000651"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8381468.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13705..15066)
                     /locus_tag="IV041_000652"
     CDS             complement(13705..15066)
                     /locus_tag="IV041_000652"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8381469.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15080..16042)
                     /locus_tag="IV041_000653"
     CDS             complement(15080..16042)
                     /locus_tag="IV041_000653"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8381470.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16068..18260)
                     /locus_tag="IV041_000654"
     CDS             complement(16068..18260)
                     /locus_tag="IV041_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8381471.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18261..18662)
                     /gene="ftsL"
                     /locus_tag="IV041_000655"
     CDS             complement(18261..18662)
                     /gene="ftsL"
                     /locus_tag="IV041_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8381472.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18667..19626)
                     /gene="rsmH"
                     /locus_tag="IV041_000656"
     CDS             complement(18667..19626)
                     /gene="rsmH"
                     /locus_tag="IV041_000656"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8381473.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19647..20078)
                     /gene="mraZ"
                     /locus_tag="IV041_000657"
     CDS             complement(19647..20078)
                     /gene="mraZ"
                     /locus_tag="IV041_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8381474.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20248..20616)
                     /locus_tag="IV041_000658"
     CDS             complement(20248..20616)
                     /locus_tag="IV041_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8381475.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20787..21743
                     /locus_tag="IV041_000659"
     CDS             20787..21743
                     /locus_tag="IV041_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8381476.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21816..21932
                     /locus_tag="IV041_000660"
     CDS             21816..21932
                     /locus_tag="IV041_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8381477.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21987..23669)
                     /gene="recN"
                     /locus_tag="IV041_000661"
     CDS             complement(21987..23669)
                     /gene="recN"
                     /locus_tag="IV041_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8381478.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23687..24136)
                     /locus_tag="IV041_000662"
     CDS             complement(23687..24136)
                     /locus_tag="IV041_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8381479.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24243..25061)
                     /locus_tag="IV041_000663"
     CDS             complement(24243..25061)
                     /locus_tag="IV041_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8381480.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25070..25960)
                     /locus_tag="IV041_000664"
     CDS             complement(25070..25960)
                     /locus_tag="IV041_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8381481.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25962..26186)
                     /locus_tag="IV041_000665"
     CDS             complement(25962..26186)
                     /locus_tag="IV041_000665"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8381482.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26191..27528)
                     /locus_tag="IV041_000666"
     CDS             complement(26191..27528)
                     /locus_tag="IV041_000666"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8381483.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27529..28383)
                     /locus_tag="IV041_000667"
     CDS             complement(27529..28383)
                     /locus_tag="IV041_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8381484.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28480..28929)
                     /gene="nusB"
                     /locus_tag="IV041_000668"
     CDS             complement(28480..28929)
                     /gene="nusB"
                     /locus_tag="IV041_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8381485.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28922..29347)
                     /locus_tag="IV041_000669"
     CDS             complement(28922..29347)
                     /locus_tag="IV041_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8381486.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29593..30657)
                     /locus_tag="IV041_000670"
     CDS             complement(29593..30657)
                     /locus_tag="IV041_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8381487.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30829..31074)
                     /locus_tag="IV041_000671"
     CDS             complement(30829..31074)
                     /locus_tag="IV041_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8381488.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31213..31587)
                     /locus_tag="IV041_000672"
     CDS             complement(31213..31587)
                     /locus_tag="IV041_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8381489.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31670..32584)
                     /locus_tag="IV041_000673"
     CDS             complement(31670..32584)
                     /locus_tag="IV041_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8381490.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32605..33408)
                     /locus_tag="IV041_000674"
     CDS             complement(32605..33408)
                     /locus_tag="IV041_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8381491.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33441..34430)
                     /gene="manX"
                     /locus_tag="IV041_000675"
     CDS             complement(33441..34430)
                     /gene="manX"
                     /locus_tag="IV041_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8381492.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34533..35024)
                     /locus_tag="IV041_000676"
     CDS             complement(34533..35024)
                     /locus_tag="IV041_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8381493.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35215..38049)
                     /locus_tag="IV041_000677"
     CDS             complement(35215..38049)
                     /locus_tag="IV041_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8381494.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38456..38749)
                     /gene="rpmA"
                     /locus_tag="IV041_000678"
     CDS             complement(38456..38749)
                     /gene="rpmA"
                     /locus_tag="IV041_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8381495.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38762..39106)
                     /locus_tag="IV041_000679"
     CDS             complement(38762..39106)
                     /locus_tag="IV041_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8381496.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39133..39441)
                     /gene="rplU"
                     /locus_tag="IV041_000680"
     CDS             complement(39133..39441)
                     /gene="rplU"
                     /locus_tag="IV041_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8381497.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            39723..40364
                     /locus_tag="IV041_000681"
     CDS             39723..40364
                     /locus_tag="IV041_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8381498.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40426..42057)
                     /locus_tag="IV041_000682"
     CDS             complement(40426..42057)
                     /locus_tag="IV041_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8381499.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42180..42539)
                     /locus_tag="IV041_000683"
     CDS             complement(42180..42539)
                     /locus_tag="IV041_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8381500.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42698..42771)
                     /locus_tag="IV041_000684"
     tRNA            complement(42698..42771)
                     /locus_tag="IV041_000684"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42735..42737),aa:Arg,seq:tct)
     gene            complement(43080..43763)
                     /locus_tag="IV041_000685"
     CDS             complement(43080..43763)
                     /locus_tag="IV041_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8381501.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43891..44409)
                     /locus_tag="IV041_000686"
     CDS             complement(43891..44409)
                     /locus_tag="IV041_000686"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8381502.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        12636 a         9657 c         7521 g        14774 t
ORIGIN      
        1 agaatgagtc ttctctatca tctttcgttt tttgttgtaa aaacgtctaa aaatcctttg
       61 ttttttattt agaaaaatag tcaattaacg ttttttttgt tacacttact aatgatggtt
      121 ttttaccatg aaaggatgat cgaatatgac accaaatcga gaagattatt tgaaaatcat
      181 tttagaacta ggtggagata caacgaaagt caacaataaa caaatcgtat ctagtctaga
      241 tgtctcagct gcatctgtca gtgaaatgat ttctaaactt gtgaaagaag agcttgtaga
      301 acattcccct tatcaaggtg tacaattgac tgcaagcgga ctgcaaaaag ctagcagtct
      361 gattcgcaaa catcgcttat gggaagtttt tttagttgaa catttagact atagctggaa
      421 tgaagtccat gatgatgctg aagtattgga acatgtgact tcggaacatc ttgcagatca
      481 tttagaaaac tatttgaatc atcctgaaca ttgtcctcat ggaggatcga ttcctaaaaa
      541 agaggaagtt gtccatgaag aacgccgaca aacattggag tcctatcctg tcggtacaaa
      601 agtgcgtatc gcacgagtgc ttgatgaaaa agaattgttg gattacttag tcactattga
      661 cctgaatatc aacgaagaat atgagatcgt tgacatcgca gcttatgaag gaccgatcac
      721 tatccaaaat gatagaaaga aaatccccgt cagctttaag gcggccagca cgatttttgt
      781 cgataaaata taaaggagtg tatccacatg tctaatgaaa aaaatgtatt gtttacgata
      841 ttcggcggta caggtgacct agcccagcgt aaattgtacc cgtctttgtt ccgcctttac
      901 agaaaaggaa atttaggcga gcattttgct gtaatcggaa ccgctcgccg tccctggagc
      961 gatgagcact accgtgaagt tgtcaaagaa acaatccgtg cattgaatcc ttcagatgaa
     1021 gaagcaacta cctttgcaag ccatttttac tatcaatccc atgatgtaaa tgattcaacg
     1081 cattatcaga cattaaaaga attgtctgac cgattagatg gacactatca attaaacggt
     1141 aaccgtgtct attatttggc tatggcacct caattttttg gaacgatcgt ttctcatttg
     1201 aaatcacaaa atattatgac aaaaaatggc ttcaatcgtt tgatcataga aaaaccgttc
     1261 ggttctgatt accagtctgc ttttgaattg aaccagcaaa tcagagaagt attcccagaa
     1321 caagatattt tcaggattga tcattattta ggaaaagaaa tgatccaaaa catctcagct
     1381 atccgttttg ccaataatat ttttgaggct caatggaaca atcgctatat cgacaatatc
     1441 caaattactt ttggcgaaaa cctcggtgta gaagatcgcg ggggctacta cgatcatagt
     1501 ggcgcactta aagacatggt gcaaaatcat attttacagg ttgtagcatt acttgcaatg
     1561 gaaccgccag tagcattttc cgaaaaagaa atccgtacag aaaagatcaa agcattgaaa
     1621 gctatccgca tttataacga agaagaagtg ttagaaaact tcgttcgcgg gcaatatgct
     1681 caaggtgaat tggatggtgt acaattcaag ggctatcgcg aagaagacaa agtggatgct
     1741 caatcagaga ctgaaacatt cgtcgcaggc aaattcacta tcgataactt ccgttggtca
     1801 ggtgttcctt tctatgtacg tacaggcaaa cgtttgactg aaaaagggac tcggatcaac
     1861 atcgtattca aacaagttcc tgttaatgta ttcaaaacat ctgtcgatga accatgtgac
     1921 gatacgacac ttccacctaa tgttctaacg atctatattc agccaactga aggattttct
     1981 ttatccttga atgggaaaga agtagggcaa ggatttgaaa cggaaccaat caaattggaa
     2041 tttagaaata gtgcagaaat ggttgaaaat agcccagaag cttacgaaaa attactatta
     2101 gatgcgttga atggtgatgg aacgaacttc tctcattggg aagaagtcgc tcaatcttgg
     2161 catatcgtgg atgtcattcg aaaagcttgg gaccaaacac aaccggattt tcccaactac
     2221 aaagctggat caatgggacc taaagcagct ttcgaactac ttgaaaaaga cggattcgaa
     2281 tggatctggc aaccagataa ctggtatcgt gaacgtggaa aattaaacga ttgataacaa
     2341 aagggggtgt gacaaagaac atgtcacacc cccttttccc ttttactttt cttcgaatcc
     2401 ttcagctaca gcttcaggat agaagtcttc aacgattttt gcacaacgac cgcatagatg
     2461 aggaagtttt tcatctactc ctacatcttt acgaacttgg cgacagcgat cgcaagtttc
     2521 cccgtctgct ttttccactg taatagcaac atcatcaaat accatagcgt tatctggcac
     2581 ttgttcatct gctgccataa cttcaaagta gtctggtgaa acgatcaata attgtgcaat
     2641 atctgcatcc aaagctttta acatttcacg aacttgttgt tttggataaa cagttagttt
     2701 tgcttccaaa gatttaccaa tcaatttgct gtttcttgct tcttccaatg cttttaagac
     2761 attatcacgg aagtccatga aagcagtcca ggtatccatc aattcttctt ggtttgcaaa
     2821 ctcttcataa ccaggaaact cgcttaactg aacgtactcc tcttcttctt tcaggaatga
     2881 ccagatttct tctgctgtat gcggaatgat aggagtcaat aattttgtca aagctacagc
     2941 tgtttgatag aaaacagttt gcatgcaacg acgttgataa tcatcttctg cttcaatata
     3001 aactacatct ttagcaaaat ccaggtagaa tgacgataga tcaaccgtca agaagttcat
     3061 aactgtacgg taaatatgca tgaagttata tttgtcgtac ccttcttctc ggatttcttt
     3121 gattacttgg ttcaatcgaa cggtcatata tttgtctaca gaacgtaaat cattgtacga
     3181 aacagtatct tttttaggat cgaaatcttc tgtattcgct aataagaaac gcatcgtatt
     3241 tctgatttta cgatatactt ctgccacttg acttaagata tccatagaaa cacgaacatc
     3301 tgcttcataa tctacactag atacccataa gcgaaggata tctgcaccaa attgtttgat
     3361 cactttttct ggtgcaatga tgtttcctaa agatttactc atcttacggc cttcaccatc
     3421 taaagcaaat ccttgagaaa taactgattt gtatggtgct acaccattga tagccacact
     3481 agtcgtgata cttgagttga accagccacg atattggtct gatccttcta aatacatatc
     3541 tgctgggaat gataattctg gtcgctgacg taaaacagct tcatgagaag aaccagaatc
     3601 aaaccagaca tccatgatat ctttttcttt tgtaaattcg ccattaggag aacctgggtg
     3661 ggtaaatcct tcaggaagaa gttctttcgc ttctttttca aaccagataa ttgatccatg
     3721 ttcagcaaat aattttgcca catgttcgat tgtttctggt gtgatgattg cttctccatt
     3781 ttcagcatag aaaatcggaa gtggtactcc ccatgcacgc tgacgggaaa ttacccaatc
     3841 gccgcggtca cggatcatat tatacaaacg tgttttaccc catgggataa cccactctac
     3901 tttttctact tcatccaaaa tattctgacg gaatttatcg atagatgcaa accattgtgg
     3961 agttgcccga tagatcacag gctttttcgt ccgccaatcg tgaggataac tatgagtaaa
     4021 gaaatctagc ttcagcaaag ctcctttttc ttttaataga tcagtgatca taggatttgc
     4081 tttgtcataa aagatacctt caaatccagg tgcttcttct gtaaacaatc cttttgagtc
     4141 tacaggtgac actacagata gtttatattt gctaccagcg atatagtcat cttccccatg
     4201 tcctggagca gtatggacta atccagtacc agcgtctaaa gtaacatgat cgcctaaaat
     4261 cacaagtgat tcacgttcat agaatggatg acttgcagtc atatattcca tttcttgtcc
     4321 agcaaattct tttagaactg ttacattttc ccaaccgatt tctgattgga ctgtttctag
     4381 caaatctttc gctacgacaa acttacggcc atcggcttcc accaatacat acgtaaattt
     4441 tggatttaca gcaatacctt ggtttgctgg caatgtccat ggtgttgtcg tccaaatgac
     4501 aaaagctgta tcttcatcaa gaatatcttt gccgtctttg acattgaaag ctacaaaaat
     4561 agatggagat tttacatctt tatattcaat ttcagcttcc gctaaagaag actcacttga
     4621 cggagaccag taaattggtt tcaatccttt gtaaatatag cctttttcag ccattttacc
     4681 aaatacgcgt acttgtgcag cttcataaga aggatctaat gtaacataag gatgttccca
     4741 atctcctgca actcctaggc gtttgaaatc tgctcgttgc ttatctactt gggaaagtgc
     4801 atattcacga catttttcca agtattctgc catggacatt tctttacgtt tcacgccttt
     4861 attagccaat acttgttcaa tcggcaatcc atgtgtatcc catcctggaa catatggtgc
     4921 acgatagcca gacattgatt ttgaacgaat gataatgtct ttactgattt tattcaatga
     4981 atgtcctaaa tggatattac cattggcata tggaggtcca tcatgaagaa caaagctagg
     5041 ttttccttcg ttcaactctt gtcttttttg gtagattcga ttttcttccc attctttttg
     5101 ccactcgcct tctcggtttg gcaaattacc acgcataggg aattttgttt ttcctaattg
     5161 caaggtttct ttcattttca ttcaaccata cactccttta tttgttttcc ggacacaaaa
     5221 aagcatgttc agccgcaagg gacgaacatg ccgtggtacc acccaaattt agttgcatct
     5281 atctagtgca aactctctgt gaccaataac gaggtcagcc gctgcttctt actctaattt
     5341 cggaaaagca ggtctcaagt agaggatctt tcaccatgca gtgtcttacc gaacttccac
     5401 tctcatcggc tcgctttaag aataacaaag tgcttttctg ttctactcgt tttcattttc
     5461 accaggcacg atcgagatat caatagcctg tgtatcaaat gccgtcatag gtgcttctat
     5521 gactcctgtg tctgagttta catcagtatc attttcattg tcaagctgtt ttgctaaaac
     5581 ttctttgatc acttcatggc tgtcagtcac gtagctggag aatggttgta aaatttcatc
     5641 ccattccgga cttttcactt gttctaattg agactcaagc atcaagctga tacgttgatg
     5701 gaatactcgt gtttttttct taagatcttc agtttctgtc gcaagctgtc ttgctttttc
     5761 tgttgcatct gtcaagattt ttcttgcttt ttcttctgca tcagttgtta attgatgtgc
     5821 acgtttttca gcgttcgcaa ctaactcatc tgctttgttt tgtgcagaag taacgatcac
     5881 ttcggattcc ttgcttgcac ttgtttttac tttatcagcc gtatcttgag ctacgatgat
     5941 tgattggttc aaagcatctt ttaattcatt gaagtactca agtttttctt cagaatgttt
     6001 gactgctttt tctaattctc gatttctttg agtcaattcc tcatagtcac gaacaactaa
     6061 atctaagaaa tcatctactt cgtcttgatt atatccccgc atttttgtcg ggaatgtctt
     6121 attttggata tctaatggag ttaaagccat gagtaaacca cctttttatt ttatttacgt
     6181 aatacaccga acagtaccct gtacttttct tttttcgtct tcccctcaag ttcttggatc
     6241 tggactctgc cataacctct gactgaaata atatctaata gttcaagtac aaaatcaggt
     6301 cttgtatttt ctgtccagtt cacttttact ttaccagctt cgatcagctg cttggaccgc
     6361 tgtctggaaa tattataaat ctcagagatc acactgtcta gtcgcatgga actaactgtt
     6421 cctctttctt gtgtccagct atctttcggc ataatcattt ctgtgtattt gattggttcc
     6481 aagcgtacag caacctttcc aatctttgtt acttgtgcag caacaaaatg atccacctca
     6541 tttgcaataa atacttgcca gcgatcaccg tccgagatga tatcgccaaa gtaagagcgt
     6601 ttgatccccg ttcccactaa agttccaagg attttcccat gacttaggct ggcaaatttg
     6661 ctaggataat ggatttcata caatgctgtt tcaaaatctt cctctacagg ttcgtaataa
     6721 tcggggaaaa tcaaacatct ttttctttct gcctgttcat atccgccata aaacatgaaa
     6781 cgcaaatcac tgctttgtcg aatcagtgtt tctaaaatat aggattgtct tgggtcaaga
     6841 aattcggtca gatagggtgc atattgtcct tgaacctgtt caatccaatc gcctaccgaa
     6901 tcgataaatg tacgttcatc tgcccgaaag tgctggtata catttgcatc caaaattttc
     6961 ccctcctatc tattctgaga ataaagattt tgttgcttca atcaataaac taaatggaca
     7021 gttatccata taacgatctg tgctaaacct tgtacggcta agttcaagat caggattgcc
     7081 cctaaaattg tgaaatccaa gggtccaatc ctcaaaggca gacgatcgaa caaactgata
     7141 tacggttcgc agatcttagc taataatcgt cctagccctg attgataccc gcctggaaac
     7201 caagagagca atgcatatac catcaagata atgctataga aataggctgc ccgttgaagt
     7261 aaatgaagta atagtgcaat agtataaatc gtagttcctc ctcttacaga tcaaacaaat
     7321 tcgcatctgc aaaagattga gcggttgaac tgtcaatctc catcccagtc ggtgtacaaa
     7381 gaaaaatctc atctccaaca cgtttgatat ctccatctag tgcataaact gttcctgtta
     7441 gaaaatcaac gattcttctc gcttgttttt cttctactaa atgaaaattc actaaggttg
     7501 cttctccagc aatgattcgt ttagcaatcg ccatagcttc agaataagct cttggttcca
     7561 ttacagtgat ttttccagca agatttgctt taggttgtgt agaggttctt gaagctgttc
     7621 tttctttcgc tacagttttt gtaggtgatt gatgtagttc aattactttt ttcttttcag
     7681 ccggctgaat actttcatgc gttacaggac gtgaagtcgc cgttttttgt ttttcatgag
     7741 tattcgcagc agatacagaa tgagttgatt gattctgtgt tgtatttttt ttagttggct
     7801 gtacagcctt ttttgttgtt tcaggataat cttggtattc gtactcttcc tcatcagcta
     7861 atccgaaaaa attagcaatt gctcctctgt ttaaaagcgg catttttgtt cctcctttat
     7921 tcgtctttga atagcgctgt tcctacacgg ataaatgttg caccttcttc aatcgcaatt
     7981 tgaaagtctt gactcatccc catacttaat tcactacatg gtgcatgggc ataacctttt
     8041 gcttgaacaa gatcacgcaa ttctttcagc gtaccaaaca ctttatggat ttgctcatca
     8101 gaagcttctt taggcgccat agtcatcaac ccaacaacct tgatttttgt gcaggcagct
     8161 aaagattcca caaacggtaa aagctcatct ggtgaaattc catgttttga ttcctcacca
     8221 gaaacattta cttcaataaa acaggataat tctttctcta accgcttctg gatttcttct
     8281 gctaatctta aactgtccaa cgcatgaaag taatcaattt catttacaat caattttacc
     8341 tttctgcgct gtaaattacc aattaaatgc cactttattt caggaaaatc aaataaatct
     8401 tgtttctttt ctaataattt atctactcgg ttttctgcac aattcataac gccagctcta
     8461 accaattctt cagcttgagc ggattcaaca gattttgtta cagctactaa tgtaacttct
     8521 ttcggatcac gatgaacaag agcacacgaa tcctgaattt cttgcttgat tttctgcaag
     8581 ttgtcagcaa tcatgtgctc ttcctctcct ttatttttta cgacggaaaa acggtggcgt
     8641 atttaattca tcatcgccat gagattttgt ttcttcgcgg ttgaatgtat caaattcttt
     8701 tttttcgata ttgtcaaaat tggaatcatc aactctagga cgaacatttt gttcacgtct
     8761 gatatcccaa tcgccaaaac tgttttcttc ttctgaagaa gttggtttag cttgatccat
     8821 atctaataca gtcttttgag caggtgattg catttgtgct tgtctagctg gtcggctaga
     8881 cttgcgttcc ttttttgatt catcgatacc tgttgcgatg accgtcacac ggatttcatc
     8941 gcccatttct tcattgatag atgtacctag aatgatattt acatcgccag ttgctgcatt
     9001 tgctacgata tctgaagcat cttgtgcttc gaacaatgtc atatccaaac cgcctgtgat
     9061 gttaagaagt acttgttcag ctccatcaat agatgtttca agcaatggag aggagattgc
     9121 ttttttcgtt gcttctacta cgcgatcttc tccactagcc acaccgatcc ccatcaatgc
     9181 tgtaccttgg ttttccatca ctgttttcac gtcagcaaag tcaaggttaa cgtaacctgg
     9241 cgctgtgatc aaatcagaga taccttgtac accttgtcgt aaaacattat ctgcttcacg
     9301 gaaagcttca agcatcggtg tctttttatc tacaacttct aataaacggt tatttgagat
     9361 aattaataat gtatccacat tttctttcaa acgagcgata ccttctgcag caaaacggcc
     9421 acgtttcggt ccttcaaaag tgaatggacg tgtcacgaca ccaacagtca atgcgcccaa
     9481 ttctttcgcg atacctgcaa cgattggtgc agcacctgta ccggttcctc cacccattcc
     9541 tgcagtgatg aaaatcatat ctgcaccgtc taaagcttcg cgtaaggatt gttcactttc
     9601 ttctgccgct ttttgtccaa cttctggctg agaaccagca cctaaaccac gagtatattt
     9661 ggggcctaat tggataactg tttcagcttt tgaatttttt aatgcttgca cgtctgtgtt
     9721 ggctgtaatg aattcaacac ctttaacgtt ttcttcaatc attcggttta cagcatttcc
     9781 accgccacca ccgactccga tgactttgat tactgcgccg tcattgatat tattatcaat
     9841 tgagaattcc atatcttatt cctcccagga tgaattaatc aaaaatattt gtaaagaaac
     9901 ccttgatttt attttttaca ttttctccgg actcttttgg ttccggctca tcgtagattg
     9961 tttcttcagg tgcttcatag ctctcatatg tgtttgttgt ttcttgttca actaccattt
    10021 gacgagctgc tgaggtttca cccgtaacag caatttttgc caattgatac acttcactca
    10081 agtttgctga ataatccacg atacttatta catttgtaaa taccgggttt ctcagcccca
    10141 tgtgatttgg aacatacaat tttacattta cgccgaagat ttcttgtgcc aagtccacaa
    10201 caccaggaag actagcagct ccgcctgtca atacgacgcc acctggcaac tctaatgctt
    10261 cgatttggtc caatgcatct tttgctttgt tgaaaatctg ttccattcga gctgaaataa
    10321 tttctgacaa gtagcgttca tcgactttca ctggttctga ctgaccaatc acatctactg
    10381 gaaattcttc atctggtgat gtccgttctg ggtatgcatc cccgtagttt attttcaacg
    10441 cttctgcatt gttaaatgac gtatttaata caatagagat atctttagtg acaaattcgc
    10501 caccttcttg gtcaagattg gtaaacttca attgcttatc atgcataaca gccgttgttg
    10561 tttggccgcc acccatatca atgacgatcg taccaaaatc tttttctccg tcagaaagaa
    10621 ttgattcagt cagcgctaaa ggagtaatca ccatttcgtt gacgattaat ccggcatttt
    10681 ccacgcattt acgtatgtta tggatgatcg ttttaggtcc tgtaaacaac agaccgtaca
    10741 tttctaatct tacgccgatc attccacgag gatctttgat tccctcgaac ccatcgacag
    10801 taaaatcttg tggcaatatt gacacgattt gacgttcagg aggtattgaa cgaacaaggg
    10861 cagctgaagc cacatttctg acatcttcat cggtgatttc ctttgaatct ccgttaactg
    10921 caatcattcc ctggcaattt tctacttcaa gcatgtttgc tggcaaaccg acacttacgc
    10981 ctttgatctg gatacctgct ttttcttctg cttgtcgtac tgctcgttgt atcgcttgga
    11041 ccgttttatc gatatcaacg ataatgcctc ggttaatccc ttctgatttt gcgtttccta
    11101 ctccaataat attcatttgg ctgtcgatat attcagccac gacaacttta acagatgtcg
    11161 ttccaatatc aaggcctaca tacattcctg tttttgccat gaatgggatt ccctcctact
    11221 tactattcca tatacaagcg aacaactagc gtccgtttta aaatatactt attaatagat
    11281 atattcactg cttccaattc taccataaat tactggtttt tagaaagtac attcttactt
    11341 tttaaagttg ttttatcaat tttcatttaa acttgacgga gttgtacttg gattatcttc
    11401 tatttgactt gtttcacttg aatcatttga atcagatgat ccgtttgtgc tagacgatcc
    11461 aggtgtttgt gctttttgcg tttctgcctc ttcacttgat tcgtttgttt gatccgcatt
    11521 ttcttcactt tcagttgttt cttccgattg gcccgattct tccgtttctt gagtgctacc
    11581 gtaaggatgc gagaagatac caacttccat gtcgatcaca cctttttcat ccatatcttt
    11641 ggctacttgc gaataatatt tcatttggtt cgcaagatta ttgatattta caataacttg
    11701 attgccgtca ttcatattca attggatcag atttttattg cttgctcttg gcgtgtactt
    11761 gatttcagaa attgcttttt gtaattctga tgataactga ttgtattgct ttgttaattc
    11821 tttgattttt gcagagtctt tgaactcttc caagataggg agattttttg taggatttgc
    11881 tactttttca tctaatactg ttccattttc aatcactggg tagtattgat tgttttttgc
    11941 gatcaaagca atctctttat attctgttac atccaaatcg aattcattca tccctttgaa
    12001 atgtatattt gctgtttcga ttcttggctg tgctttttta aggcgatcga tataagtaga
    12061 tcgatgaaaa tactgtcccc aaacattttc tcctaatgac aattttgtat cagagatgat
    12121 ttcttggctg ttgactgtct tattgcctga tactgcaact gcttgtaatt tgctataagg
    12181 agaaacataa tagatcaaaa aaatcagcgg tatacccaat acagtgatga tcaaagctaa
    12241 ccgacgaaat agtactttgt gccgttgact ttttaaattg ggcagccggt tcaaaaaaga
    12301 accgttatat ggtccaccgt ttttcttttg cttgatctct tcaaaaaatt cgattttttt
    12361 ctcggactgg ctttcttctt gctgttcctc ttgaaccgtt ttttcttctt cagaatctga
    12421 aaggggtgta tctaatgtta tgtcttcttt ttttggctct tggtttttca tctcttgttc
    12481 tgccagatat tttcggttag ccttttgcca aggagtcaaa ttgttttcat cagaagacgg
    12541 atgagacaat ttttttggtt cttttttctt actcactaag accaccgcct ttattttttg
    12601 attatttgtt ttactaagtc ataaagacgt tttgaagcat cagggattcc ttgttcttta
    12661 gaagctcggc acatttgctt ttgcaattct tcatcaccca tgatttcgtt gactgtctga
    12721 cttagtgatt cacctgttag ttcattatct gcaatcattt ttgctgctcc tgcgtgaaca
    12781 agactcattg catttttagt ctgatgatca ttcgtaacat atgggctggg aaccaacact
    12841 gccggtaacc ccagagctgt gaattctgcg atcgaagttg caccagcacg ccccaccagc
    12901 agatcactgg aagccatgac ctctgccata tttttgatat aaggctggac actaatattt
    12961 tgtatttcag gatttatttt atttaggatc tcttcataat atctatctcc tgaagcatac
    13021 aaaacttggt aagaagaagg aatatattca cttaaaaaaa ctgatactgc ttgattgatt
    13081 ttaagtgctc cttgactacc gccaaaaatc aagactgttt tcttttctgg atccaaatca
    13141 aatgttttca aaatatctga ttttttagta tctgctactt cttgtgctcg aggattacct
    13201 acaagaactg ctttgttttc aggaaaaaag gaagccgcat ctttaaatga aagagcgatc
    13261 cgatccacat atcggcttaa aaatttattg gtaacacctg gtacactgtt ttgttcatgg
    13321 ataactgttg gaatacccat tttggctgca gcgtacacta ccgctccgga aacatatcct
    13381 ccagtaccga taacgatatc tggttgaaaa tcactgagga ttttttttgc ttgacggata
    13441 ctcttaagaa acaatcggac cgtcttgatg ttatgcatgg aaatttttcg tttaaatcct
    13501 tgaatctcca aggtatggaa aggcattcct gtatcaggga cgattttgtt ttctagacct
    13561 cgttttgctc ccacatacat gaattcagca ttcggttctt gagtctttac ataattgaca
    13621 aaggcaagag ctggataaat atgtccccct gttcctccgc ctgtaaccaa aattttcatt
    13681 ttttatcgct cgcttctact ttttctattt taatcggttc acagcttcca tgaatctgtt
    13741 tcctctagtt tcaaaatttg ggtattgatc ccagctggca ttcgctggtg ataataaaac
    13801 tacatcacca tctgtactgt tttctaatgc taaaggaact gcttgttcta cagaatcggc
    13861 tgttactatt gtctcgatgc ctgctttttt tccagcatcc atcaactttt cttttgtttg
    13921 gccaaatacg accattgctt tgattccttt taatgaagga attaattcat caaatgtatt
    13981 tcctctgtct aatccgccag ccaataacac gaccttagac gtttcaaatc cacttagagc
    14041 cattttagtc gctaaaatat tggttgcttt tgaatcatta taaaattttc tgccttggat
    14101 ttctcctaca tattgcgtac gatgtggtac gccatggaaa aaatgcaacg tttctcgtat
    14161 agcttcattt gatataccat acaattttgc tacagaaata gcagctagtg cattttctac
    14221 attatgctta cctggtacgc caagttcaga tatttccatt attttttctt ttttatagta
    14281 tattgagtaa tcgtcagcat acacaccgtc ttccaacact tcttttgtcg agaaaggcag
    14341 tacgcgtgct tttgttcttt gcgctaattc ttgtagttct gactgattcc agttaaggat
    14401 cagataatct ttttctgtca tgtttttttg aaggttccat ttagctttga catattcttt
    14461 tcttgtacca tgataatcaa tatgtgcttc atagatattg gtaatcaccg ctatatgagg
    14521 gcggaaatct gtgattccca tgagttggaa gctagacaat tccatcacaa ttttatcttc
    14581 tgattttgct tcttgcgcta caccacttgc tggataccca atattacccg ctaaacgtgc
    14641 aattccttga tctgctcctg cattcagtag tagtcctgtc atcgttgttg tcgttgtttt
    14701 tccgttcgtt cctgtaatcc caataattgg gcattccgct acttcgtaag ctagctcaac
    14761 ttccgtaatt accggaatac ccatttcttg ggcttttgct actaaaggat gactgtaggg
    14821 aatccctgga tttttgacca tcaatgaaaa ttcttcatcc aacaattcga ttggatgact
    14881 acctgttatt acttttatcc ctaaagaaag cagctcttgt gcttccggat tttcgtcaaa
    14941 aggttttcca tcattaacgg tgacaagtgc gcccaattca tgtaataatt tggccgcact
    15001 tactccgctt tttgcgagtc ctaagacaag cactttctta ttttcatatg ttgatatttt
    15061 tttcatttcc cgtcatctcc taccaaacga tccataatgt gatcaacgaa gtcacaatgc
    15121 tgatcaacca aaaaactgta tcaatcttcc actccgacca tccgcacatt tcaaagtgat
    15181 gatgaattgg agacatttta aaaattcgtt ttcctgtaag cttaaaggaa gtaacttgaa
    15241 gcatcacact tgctgtttcc ataacataga tcaaaccaat caaaagcaat gtccattctt
    15301 ggtttagcat tatagaaatt gccgctaaaa gtccgcccaa cgctaatgaa ccgacatctc
    15361 ccataaaaat cttggcaggt tttctgttgt agacgaagaa acccagtaaa cctcctaaaa
    15421 cacttaagca aatgattaat acatcatact gatgctgatg ccaagcaatt agaccgtaag
    15481 ctgcaaaaga gatacttcct aagccagcaa ctaatccatc gatgccatct gtcagattta
    15541 ctgcatttga aaatccaaca agccagaaga gagcgaaaat accataaatg attcctaaag
    15601 gtagatctag tccgaagaaa ttcaatacac ctggataacc ttcagaacgg taaacaagat
    15661 aaaagatgat tccccctaga atctgtccaa tcaatttctg cttcgagttc agtcccatgt
    15721 ttcttttctt gaatattttt ataaaatcat caagaaagcc gattactccg tataacgcta
    15781 atacaaaaag caagatgaaa agagtaggag ttagctgttt ttgccaagct ccaacccaaa
    15841 ttcctgtcaa aatagagccg atcagaaaca ctaagccgcc cattgtagga gttccggctt
    15901 tgctattgtg ccactttggt ccctcctcac gaatcgcctg accttgcttt ttcatttgaa
    15961 aataaccaat aaatagcggc attgcggcaa ttgtcattgc acaactgctg acaatgggaa
    16021 tcaatgcttg tgtccattcc atgctctttt ctctcctatt tctcttctta ttcttctaaa
    16081 gtaaaactaa gtttgtcttc agtgattttt tcatatggag caagttcttg tttgacacaa
    16141 taaccatctc catcaaaact aacttcaata ccgaggattt tccccagctt catgatatct
    16201 gcttttgacc atcctgttac gtcaggcatc agcttatcac caccagtata taggatcagt
    16261 ttttctccag agatcaactg atcaccgttg gcagtagatt gtttttgtac ttttttacca
    16321 ttaccgatca ctacaggttg cagtccactt ttttgagcat cagctgctgc aacgtccgct
    16381 tcaagattac gataatcggc tacggatact ttttctgttt ttgtttctgt atctgaatct
    16441 tcttcagttt ctttaaaatc cattgcccgt ttcatcaatg gatttgcgat tttagccaat
    16501 gccattcgat catatgtctt agggtgtttc attgttagat acaagacata gtctggatct
    16561 tcagaaggaa ccatctccac aatcgaatac aggtaggctg tatctcctgt ttgatatccc
    16621 cctgtatcag aagcgatttg ggctgttccg ttttttgccg atacattgta tccaggtaca
    16681 ctatatacac cataagcact accgtagttt tcgctttcga caacatctct catatattca
    16741 cggacttttt ctgttgtttc ttttgaaaaa ggttgcccta gaacttctgt ttgagttgtt
    16801 cgctcttctc cagtttgggg atccaccact ttgctgatat atcgaggttg aatcattgta
    16861 ccgttattcg caatagaagt gaaagctttc atcatttgga aattcgtcac acctaccgct
    16921 tgtccataag cactcattgc tcggtcgaca atatttaatg taggtaaagc accgtttact
    16981 tcatcatcta atccagaatg agtactttgt ccaaatccca gtttttgtaa atagttatac
    17041 catcgtccgc ctaatcgctg ttccagtatg accatcccga cgttacttga ccatgaaaga
    17101 gcttgacgca tggtcagcac acctttttca ccaaagtcat ggtcattgat cgtagcatct
    17161 tctacttgga tttttccaga ttggaaggtt tcattctcgt tgaattctcc ttcttcgata
    17221 gcagctgcag tagtgaaaac tttcatggtt gatcctggtt cataactatc ttgtaccaag
    17281 aaattacgcc agatagcatc ttttccagtc aatccttcca ttgtttcagg attgaaagta
    17341 ggtcgttgtc ccattgccaa gatttctccg gtttttgctt tcatcaggac tgctgtcaat
    17401 tcttctggct gatattcttc atttacttga tccatcagtg tttctaaata actttgtagt
    17461 ctgctgtcta aagtcgtgta gatatcttgt ccgtcgacag ctttttcttc ttctgccact
    17521 gtacctggca gaggattttg aaaattatct ttttgataaa taatcttacc atctttaccg
    17581 ctcaagatat cattgtaagc agactccaaa cctagtttac caactaagcc attctcatct
    17641 ttgtctggaa ctgcgtatcc aataaaatga gaagaaaaga cgccatttgg atacatccga
    17701 gcctgatggt cgacaaaata aagaccagcg acaccttctt ttttcatgtc cgcttcgatg
    17761 ttttgcttgg tttcttggga gatatttttc ccatagctac caaattcaac ttgatacagc
    17821 tctttttttg cgccgtcctc taaaatattc agtgcatctt ttttgtctat agaaagattc
    17881 ttatgcagga tttctgctat tttatcaaaa ttcttttctt caacatacag ttttttatcc
    17941 ccagaggtat atgtttttga gagtatcgct ttaattgaat aagaggaggc atcttctgcg
    18001 agcgccacac cgttacgatc ataaatcgtt cctctttttg cttttactac ttcacttcct
    18061 tggtacaact gttttgtttt ttcagctaat gaggtaccag caacatgacc tccaataacg
    18121 atatacgaga aacggacggc aaataaaaag aacaatccaa tactcgtagc aaataaaatg
    18181 atacctactt ttttgcgatt gttcatcgga tttagatttt tcttttccat aaaccgccga
    18241 attttatttt taagactcat ttacttcact ttccttaaat tgtcgtcatt aatagacaat
    18301 cctttttctt ctgcgatttg tttaatccgt tcagtacgag aaagttcgtt tttttcttgt
    18361 tctaaactcg ttttttcttg ctttttatca ttgatttctg tttgtattga cgtgatttct
    18421 ttttcagctc cgctgatatt tgtgcgcagc ataactgtta aaacacataa agcaacaata
    18481 gcaatcgcca cgcttgccac agcaattttt tccaaagcag aaatctgctt taatcgtttt
    18541 gccggtgatt gtggaagaat gatttcttga ttcgaagaag aatcaggcat ttgttgttcc
    18601 ggctgatcca ccaattcagg taaatcatat tcgtaatctt gcattttctt taattctgcc
    18661 atcattctac tcctctttca ttcttatttt ttctgcaata cgtaactttg cactgcgtga
    18721 tcgattgttt tctgttagct cttcctctga aggaaggatt ggtttacgag taatgacttt
    18781 cagttctggt tggaattcgt caggtatgac aggaagtccc ggtggtagat cttgtaccgt
    18841 actatattct ttaaacatac ttttgacaat tctgtcttcc aatgaatgga atgtgattac
    18901 gctgattctt ccattgactt tcaataaccg gattgcctgt tctaaagagg cctcttcggc
    18961 accgagttcg tcatttactg caatccgcac agcttgaaaa attctttttg caggatgccc
    19021 gccttttctt cttgcgggag ctgggatgac ttgtttaatg atttcgacaa gctcaccagt
    19081 tgtttggatt ggttttgttt ttcgtactcg ttcgatttca cgagcaattt gtttggaaaa
    19141 tttctcctcg ccatagcgga agaaaatctt tactaattca tggtaggaat attcattaat
    19201 tacgtgataa gcggataaag gagctgactg atccatgcgc atatccaacg gtgcatcttg
    19261 atgataacta aatccacgtt ctgcttcatc tagctgagga gaggaaaccc ctagatcata
    19321 caaaatgccg tctaccttat ctacatgctc actcaagcgt tcttctaatt ctctgaagtt
    19381 cgctttgata aatgtgacca taccttgttc gacataagaa gctaaacgca gcttggcgtg
    19441 atcgattgct ttttgatctt ggtcaaacgc atacagatgc cctttagaac ttagttggct
    19501 taataaatat tcactatgtc cggcaccgcc gagtgtacag tctacataaa cgccatccgg
    19561 tttgatgttc aatccatcta ccgtttcttt caacatgact gtatagtgtt gaaatgtttc
    19621 agtcattagt cagcctcttc ctattcttat agtccgaaat caatcatact ttcagcaatt
    19681 tcgtcaaagt tctcttctgc tgctgcagaa aaatcttgcc atcgtgtctc atcccaaatt
    19741 tcaattcggt tagatacacc gatgactacg caaccttttg tcaaagctgc ataatttctt
    19801 aacgtgctgg gtatattgat tcgtccttgt ttatcaagtt cgcattcagt ggcagccgaa
    19861 tagaagaatc gcacaaaagt acgtgcgtct tttttagcca acggcatttc gttcagttta
    19921 gcttccagat tttcccattc gctcatcgga tagccgaaaa gacagccatc caatccgcgt
    19981 gtcagaacaa acttttcgcc aagttcttca cgaagttttg agggcacgat taaacgtcct
    20041 tttgcatcaa tattatgttg gaattcaccc atcaacattg aaagcccccc attcctccac
    20101 taactaaaat tagtctacca caatccccca ctttctacca ccttttctgg gaaatccggg
    20161 aacaactttt tttattcata aacatttttt taaaaatcaa gctgcaaaaa gccttttaaa
    20221 agacttttta cagcttttct atttttttca taggtaagaa agtatgttca caatgatcaa
    20281 gacagcgtac aagacgattg tcaacagaaa aaccaaacgc caagtcatct tgaaataacg
    20341 cggataaata atttcttcat aataatatgc ttggaaaaca gcaatacaaa ttcccattag
    20401 caaaacagaa ataaagaaaa acggtaagat agaaattgtc cctgtatttc tagacaatgc
    20461 atgcataccg ccccacaaaa aaggaatcgc caaatcaggg gcttttattt taaaacgacg
    20521 atttaacgaa aataatttta caagaaattg gcaaccaaac aaaacgatta ccgggtaaat
    20581 ataccaaaac aagataagtg gtgtgaatgt gcccatgaac gtaaactcct tttttatcgt
    20641 tgcttcaagt atactgtata tactttacac aactgtttca ttttatttaa atttatgcag
    20701 ttatattcta tatcttacaa acacattcca tgtattccaa ttccctatcc gttataataa
    20761 aagaaacagg caacaggagg aaaatcatga tcaaatattt tacattagat aatgagaaac
    20821 tgcgtaatac cggaaaggaa actgaccaaa cgatctggta ttgtgtagaa cgtcctaatc
    20881 aagaagagat tcatcagctg acacatcaat ttgaaattcc gtcagattac atcacttcca
    20941 tattggacga tgcagaaaat tcccgtacag aagaattcaa tcaatcaaag ttgacaaagc
    21001 cagcgttatt attgttacaa tatccttttg ctaaaacaag tcctagcggt tattttcaag
    21061 tggatacata cccgctctct attattttga cgcccaaaaa gaagcttatc acaattacga
    21121 atcatgaacc actgttttta aagaaagtct ttagccagac ctttccaaaa agtgatattt
    21181 cgcctaatct caatatcttc tttcaattca tttggcaggt cacactctct tacaacagtt
    21241 atttagctac gcttcaatct caatgcgata ctttggaagc caaacttcaa gtatccactg
    21301 aaaacaaaca actttatcaa atcatggata tccaaaaaag cctcgtttat ttcagagaag
    21361 cgacttctgc taatttagat gcattgaaag aactagcgca aaataaactt ttccaagaaa
    21421 atcatgcttt gaaaaatcat ttacgtgatg tattggtgga aacagagcag gcaatgacta
    21481 cttctcgcat tcaattaaaa ctagtagaac aaatgaatca gactttttca gcaatcgtat
    21541 cgaataatct gaataatatt atgaaaattt taacttcttt aacgattatt ttaactatac
    21601 cgacaatcgt cggcagtatc tatggaatga acattaagtt acctattgct gaaagggatg
    21661 atgcctttat atggctagtt ctattaacat tatttatttg tatcgtaact acctattatt
    21721 taaaaaaagg gaagttttta taaaaaagcc ctcttgtgga aatggaaaaa agattgtaca
    21781 cttgaatcaa ctaattaatg acgaggttga aaacaatgcg tgaaaaaaaa caaacaagta
    21841 cttttgctaa agtaacaaaa tttgtcattt ggacaatgct gattttaacg attggatctg
    21901 ttgtattgac tgctgtaatg agtgtcatgt aaaaataaaa tatagctatc agctgctgaa
    21961 aaggacctga accactgcga ggtggctcag gtcctttttg cgagtttcaa tagttctttt
    22021 gcatgttcga gtgtgagttt tgtaatctca cttccagcca gcatacgtgc aatttcttct
    22081 tctctttctt ttgttttcaa ctctgaaaca gaagtttgcg tacgtccatc aatcactgct
    22141 ttcacaatat aatattgata gtcggcgact gcagcaactt gaggcagatg agtgatgcac
    22201 aaaacttgag agtatttcga tatttgagaa attttatctg cgatggcctg tgcaactctt
    22261 ccgctcacac ctgtatccac ttcatcaaaa ataatacttg tcacaccttg ctcagaagaa
    22321 aaaatagttt tcaaagcaag caacatccga gacagttcgc caccagaagc gactttcacc
    22381 aaaggcttca gtggctctcc tggattggtc gtaatgtaaa attcgatttg gtcaaatcca
    22441 tcatgattca attgtctatt ttcttctttt aagaaacgta cctcaaactc agtgttttcc
    22501 atatacagac ttttcaattc atgcagaatg gatttttcca attcttttgc gattttttta
    22561 cgcgcttcat gaagatctgc ggcttgttgc tgaagtagaa gctctttttg ctctagttcc
    22621 tctttcattt tgtccaattg accttctgaa aaatcagaag aagctagctc ttcggtgatt
    22681 tcgtcatagt aagccaatat tgactcaatg gattcgccgt atttacgttt caactgtcgg
    22741 attaattcta aacgttgcgt aacttcttct aaacgactct catctaactc caataaatca
    22801 atctgacgac tcatatcgcc gattgcatct tgcagcaaat agtaagcact ttggatattt
    22861 tcataaattg cttcatactc cacatctaga tgggcgatcc cttgcagttc tgaaacggcc
    22921 aagccaacac catctacact gttttgttca ttattcgtca gtgctccgta acctgcagct
    22981 aatccatcag caattttttg ataattgcta agtttttctc tttcttcttt taatttctct
    23041 tcttctccga cttgcaaatc agcagcagct atttcttctt gctggaaatg gagcatatct
    23101 attctctgaa cataggattt ttcattttgc tgcacttttc gtaccctttt ttccagctca
    23161 cgataagaga tatattcttg ctgataactc tttttcttct gttggaaagc tacatctcca
    23221 aaacgatcca ataaagcaag atgtgattca ggctggagca gttcctgatg ttcgttttgt
    23281 ccttggatat ctactaaata actgccgatt cttctcagat tagctaatgt gataatatga
    23341 ccattcactc gacaaacatt ttttccagtt aaggacatat cccgtcgaac aatcagattg
    23401 tcctcatctg tttcgatccc taattcaacc atcagttcag agaatccttc ttgttttggt
    23461 agttcaaaca acccttcaag cacacatttt tctgctcctt gacgtatata atcgcttgaa
    23521 cctcttcctc ctgctaacag acctaaagca tcgataataa tggatttacc cgcaccagtc
    23581 tctccggtca gcgcggtcat tccttcgtga aaagaaagcc gcaattccgg aataatcgca
    23641 aagttagtaa tactgatttc ttgtagcatc acattcgctc ctcaacttaa aggtaatgca
    23701 ggaagtcttt cttcaataaa actgcctggt cttcagttcg tgtaatcatc aaaacattat
    23761 cgtcatcatt gataatcgaa aataattctt tcttgtaacg tttatcaatc aaatttgctg
    23821 ctgccgaagc atttcccggc aatgttttca aaataacaaa tttttccatt tggtcaactg
    23881 ccacgaaagc gtcttttaat agtttttcca atttagtatt tacatcttcg tttgtttcag
    23941 ctggtatgct ataacggtag ccgccatctg aagccggtac cttgatcagt ttcagttcct
    24001 ttatatctct ggaaatcgta gcttgagtaa cagatattcc tttgcttttc aggatttcaa
    24061 caaattcttc ctgctttcgg atatcttgct cactcaacaa tcgagtaatc aaacgatgcc
    24121 gatctttttt tctcattacg tcttcgctcc ttagcatatt cttcttaatc aatgatagct
    24181 tatttccttg gtataataaa gaagaatccg catacttctc acttgatttt cacagttaca
    24241 atttatttat ggtcaaactg ttcgtgtgca tttgaaacca cttctgccat attaatagct
    24301 gaattgattg ttccgctttc gggtactttc ctcaagtgag ccaagaactc gatatttcct
    24361 tctccccccg tgatcggaga ataacttaat tccagcacat catatccacc ttgtgccgca
    24421 aatgacaaaa tgtcttctaa aactttttga tgggtctgag ggtcacgaac aattccgttt
    24481 ttccctacag cttctctgcc tgcttcgaat tggggtttga ttaaagcaat cacttctccc
    24541 tcttctttta aaatagcatg taatggaggc agcatcagtt ttagtgaaat aaaagaaaca
    24601 tcgataacag caacttctgg aattcctaag gtaaagtctt cgggttttgc ataacgaaaa
    24661 ttcactcttt ccattacgac gactctagga tcttgtctta ttttccaagc tagctgattg
    24721 tagccaacgt ctaaagcata actcattttt gcgccatttt gcaaagctgc atcagtaaat
    24781 ccgccagtgg atgcaccaat atctaacaat gtttttcctt caacagatag atcaaatgct
    24841 ttgagtgctt tttctaattt taatccccct cgagaaacat aaggaagttt tttacctttg
    24901 actcttaatt cactactcac tggaattttt tctcccggtt tatcaaaccg ctcgtttttt
    24961 tcgttgtaaa tcaaaccagc catcactgaa cgtttggctt gttctcttgt ttcaaataat
    25021 ccttgtttga ccgctaatac atctacgcgt tctttttcca ttctttccct catttctcca
    25081 catcaaattg cttgatcatc tggcgcatca gcccgccatc aaaagaaaaa acgtcctctt
    25141 ctaatttgtc tatgatcttg ttggcggata acagctcaat ctccaacgcc tcacgtgtct
    25201 tttctaggcc taataaacgc gggtaagtat ttttttccat acgttcatct ttaccagctg
    25261 ttttccctaa tgttttcgtt gtaccaatca catcgagtag atcatctctt atctgaaaag
    25321 ctaaacctaa atgttcggct aatcgttgaa gtagcaataa gatttcttca ggttgcttcg
    25381 ctaatatgcc accagccagc aaggcataac gaatcaaatg ccctgtcttt ctttcatgaa
    25441 taaatgctaa ttcttctaaa gaaagctttt tcgattcgcc ctcgatatct gcagcttgcc
    25501 cagcaaccat accttgtgat cccgcacaaa cagctaattg ctgaagcagc agaagcttag
    25561 gagaattacc taagtgtgcc atactgatta gctggaatgc tcctgtaagc aaaccatctc
    25621 cggccaatat agctaatgct tcgccgaata ctttatgatt ggtaggtttt cctctacgaa
    25681 gatcatcatt gtccatcgca ggcaaatcat cgtggatcaa ggaatacgta tgcaccattt
    25741 ctaaagcagc tgctacttga tagacgggaa catcaatagg ctcatcaaag gaagcaacgg
    25801 ccgttaaaag taataaggga cgtattcttt tcccgcctgc tttcactgaa taactcatcg
    25861 catcatagag tcctctatcc gttgtatgct catctaaaaa agacaagatt tctttttcta
    25921 cagcaggtag atgaatagct gaaaagtcag ttaatctcat ctcagctttc ctcgtttccg
    25981 tcaaaatcaa cttcttcatt tgattctgtc atcattttcg tcaaggtctc ttctgcttta
    26041 gctaatgtat cttggcaatg tttgcttaat tccattcctc gtttaaacga atctagagct
    26101 gattctaatg gaacgtctcc ttgttctaac tgcatgacaa ttttttctaa ttcttgcagt
    26161 gactcttcaa aagttggatt ttccatattt ttattctcct ttgtctatct cttgtacttg
    26221 tcctttaacg attccatctt gataatggat cgtcagcaaa tccccttgat gaatctcggt
    26281 gacacttttt actacttgat catttaacgt cgtatagctg tagcctcttc ccatgatttt
    26341 caatggactc aacaaatcaa gagattgaac agcttgagca aaacgctgct gctgttgttt
    26401 catatattgt tggattttct cttctaagcg tttttctaga taagcagttt cttggcgagc
    26461 tgctttaact cgataaagcg gagccacttg ttccaattgg tggcttaatg cgatcgtttg
    26521 tttttctttc gcatgataaa tttgctgtgt agcttgatat aatcgctgtt tcaactggtc
    26581 taatttgatg gtctgacctt catacaatct ttcaggctgc cgaaatacat aggaacgtcg
    26641 agcacgctcg aaccgttctt gtttgcgctg gatctggcgt acaaacgcct gctgcaatct
    26701 agcttgccgt tcttgaatgc gcatcaactc ttctgacaaa acaggaacag ctaattctgc
    26761 agctgctgtt ggagtggcag cccgaacatc tgctactaaa tcagcgatcg tggtatctgt
    26821 ttcatgtcct acagatgaaa tcaccggcgt attagcttga taaatcgccc gagcaacccg
    26881 ctcttcatta aacggccata aatcctcaat cgaaccaccc ccacgaccga tgatcattgt
    26941 atcgaaattg cccatctctt ctactctctg gatattcctg acaacattgt ccgctgcttg
    27001 gtttccttgt acaactgttg ggaacaaaac caattggaca attggatatc ttcttttcac
    27061 cgtcgtgatg atatcgcgga taaccgctcc gcttggactg gtaaggaccg cgattcgctt
    27121 tgggaaacgt ggcagcattt ttttaggccc ttgaaaaagg ccttccttca tcagcttttc
    27181 ttttcgttct tccaattctt gatataaagc acctattcca tcaggctcca tgtgctctac
    27241 atagatttga taagaacctc ccgcttcata taatgaaata cgcccaacga caaggacttt
    27301 cattccttct tttggctgga atcgtaattt ttggaatgct cctttgaaca taatagcaga
    27361 tatttttgca tggtcatctt ttaagctgaa atattgatgg gcattggcac gtaaacggaa
    27421 attcgatatt tcgcctgtta aatatacgcg ttctaaataa ggatcggcat caaattttct
    27481 tttcaaatac ttcgttaatg tagtgacagt taaatattct tgtgccatct attcacctgc
    27541 tccaaaagct tcggcagatt ttacggtttg atacatcagc atcgtgatag tcattggccc
    27601 tacacccttt ggcacaggag tgatccagcc tgcaataggc tccacttcat caaatttcac
    27661 atcaccgatc agcttaccgt tagcatcacg attcatccct acatctatca ctacagcacc
    27721 tggttttacg aattcttttg tgacaaaatg tccacgtcct attgctacga ctaaaatatc
    27781 tgcttcttgt gccagttcag ccagattttc ggttttagaa tgagcaacag taacagttgc
    27841 atctgccatc aagagtagct gagccatcgg tttaccgacg atattgcttc gtccgataac
    27901 tactgctcgt ttacccgccg gatcgataga ataagcttca aacattttca tgatgccgta
    27961 tggagtacaa ggaatcttgt ccggtttacc cgcaaggagt ctgcctaaat tcatcggatg
    28021 aaaaccgtct acgtcttttt cagggcggat agctaacagg atcttttctt catctatatg
    28081 ctttggcaat ggtaattgga ccaatatacc atgaaaacta ggatcttgat tgtatttttc
    28141 aatttctgca agtagttcgt cttcagaaat cgtctcagga taacgctcta ccaatgaatg
    28201 gattccgatt tcttttgcta cacgttcttt atttcttaca tagatttggc tggctggatt
    28261 ttcccctacc aataaaacga ctaatccggg atggatacca ttatctttca atttttggat
    28321 tttttcagca atctctgcct gcattttttc agctaattct tttccgttaa tcaattctgc
    28381 catctaacca ctcccagata ttttttgtgt tttcattcta ccatagaatt ttcccgattt
    28441 ataacaggtt tatgatgatt catagaaaaa agctaggact tacgccccag cttctaattc
    28501 tttcaataca ttggataaaa caccgttgac gaatttacgt gaacggtcat cactaaatgt
    28561 ttttgccaac tcgattgctt catttaaagc aacagccgct ggtacatctg tcacataagt
    28621 catttcaaaa atcgcgatac gcaagatcac caaatccatt tttgagatcc gattgatgtg
    28681 ccaattgttt tttaaatgtt tttcaatcac tttatccaat tcatcttttt ttgcacagac
    28741 accgccaact agcgtatcaa gataaacagg aacaaactct gattcatctt cgttgatcat
    28801 gtcgcggtgg tctaattcga ttgcgttgaa aatcgcatct tcttttgtta ggtctgcatt
    28861 gaagtctaat ggaaataaag cctgcaatgc catttcccga atctcgtgtc ttgataattc
    28921 tttattcatt ctcttcctct tcgtcttcaa aaagttcttc gattgttggt tgttctaatt
    28981 tttcaggaac aacagctact acatggatat tgacttcaga taaatcgata tccgtcatga
    29041 aaaggacttg ctggctgacc cgttcctgca tttccattgc tactttagga actgaaacac
    29101 catattctag ataacagtaa agatctactt tcaagccatc ttcctcgttt gctaagtaaa
    29161 caccttttcc gtgagctgca cgtcctaata attcagtgac attgctggca aaagttccgc
    29221 gcattccgta tacgccttct actttagaag ctgcaatccc gataattact tcgatgactt
    29281 caggagcgat cacgatctct cctaaatctt ggtttgcgtt taaaatcaaa tttttttcat
    29341 ctgccattta tttattcctc cctttgccta agcacgagat acatccgaat tctctatgat
    29401 gcggcatagt gatcacgtgg taagaaaact gccgccgatc agctgcaaac tgctatgaat
    29461 tggagaaaac acccaacttt attatctctt acagcttaaa agttcttcta tagtgtatca
    29521 ttaaatcgag tgattttcta taacaaagtc tgtgaacact gatttttacg cttacttctt
    29581 ctattgatga aactataaga tgatcaattc ttttggtgaa cgagtcaaca cacgatttcc
    29641 atcttctgtg atcaacaagt catcctcaat acgtactcca ccgattcctg gtaagtagat
    29701 ccctggttca tcagtgatca catttcctgg tacaaattgc ttttctgcac gagaagaaac
    29761 gtttggtcct tcgtggatct ctaaaccgat cccatgtcct gtagaatgtc caaatgcttc
    29821 cccgtaacca tgttttgaaa tataatctct agcaactgca tctaattgga caccagtcat
    29881 tcctggtttt gcagcatcaa taactgctaa ttgagcatct aagactattt gataaatttc
    29941 ttttaatttt tccccaggat caccaatagc gaatgtacgt gtcatatcgg aaacgtaccc
    30001 ttcgtaatag caaccaaagt ccaatgtgat caagtcacct tgttcgatga tttttttgct
    30061 tgctacacca tgcggcatag ccgaacgcaa tccgctagca acgatcgtat caaaagaaac
    30121 acctgaagcg ccaagagaac gcatataaaa gtctagttga tttgcgactt caatctcagt
    30181 catgcccggt tggatcattt tcaggatgtg atcatatgcc atatccgcaa tatgacatgc
    30241 tttttcgata atagcgattt cttcttcatc tttcacttct ctgagttctt cgatcatctg
    30301 tgaaacaggt actaattccg catcgatgat ttcttctaaa accgaatatt ctaaaaatga
    30361 gactgttgtt tcctcaaaag ctaatgagtg cagttcttct ttgcgtacta agtttgctac
    30421 ctcatcaaaa ataggtccga catttttaat aatttcaaac ccttgagcct gcgctgccgc
    30481 ctgctcggta taacgaaaat cagtaataaa aaatgctttt tccaaagtaa taacagctaa
    30541 tcctgtcgta cctgtaaagt tcgttaagta acgaagattg tacggactgg taaccaaaaa
    30601 tgaatccaaa ttttcttctt gcattttttt gcgcaatttt tctacacgta gcatcataat
    30661 gaaactccct ctttctttgc acgctttttt ccattatagc aaacttttct tcgtacacga
    30721 cagttaaata aaaatttccg gaaaacttct ttttatttta aataaataaa gccaaaaagg
    30781 agtcacagtc tcgctgcaac tccttttagt ttactatttt gtaaaaactt atgatcgttt
    30841 tccaaagaaa aatgagacaa ctgctaccaa aatcactgcg cctaaaatag atggaataag
    30901 tgccatacct gcagcttttg gtccccattc gccgaaaatc gcttgtccaa tacttgaacc
    30961 gactagtcca gcaatgatat tcgttacaca gcccatcgaa tttcctttac tggtgatcgc
    31021 acctgcaatt gctccgatca cggctccaac aatcaatgtc cataaccaac tcataatata
    31081 acctccttct ttgctaatca attttagcat atgggattta aaggggacaa ataattggtt
    31141 ttgctatttc atattttctt ctgcaaagta aaaaaataaa gaaaacctcc tcttagaagt
    31201 tttctttatt ttttacttgc gtttaaacat actacgaata ccacgtttca ctacatcaaa
    31261 gaaactcaat gattgtacca ttctatcttc atctacatat tctctgatcg cacgaagcac
    31321 ttttttaggt tctttggaag aaaatgtgta tgtaccattt tttttcgttt gaatggcata
    31381 tcgaggaatc cattttccct ttagcatgac tgatgctagt acataatcca cttcttccca
    31441 tggaatttgg atatatttgc gtctatctcg cgcatggtaa aactcaaaac cattatcacc
    31501 tatcatgatt tgcccgtaat ctgtcagacc cgtaaacgaa gtagcatcaa ttaccaagtc
    31561 tacttttgta ttgattgatt gaaccatttt ctccctccag atatttgttt atgaacagta
    31621 tactatataa aaaaagctcg ggacaacgcc cgagctatat ttttatggat tataaaagtc
    31681 cgatgacgtg tccaacaaca ccgactacga ataaaccaag gatgataacg attggagaaa
    31741 cctttttctt aagcaaccac atacataaca atgttaatcc taatgcagct aaaccaggaa
    31801 tcaattggtc taagttgtct tgtaatgttg ttaccttttc aggagaaagg gctaagccat
    31861 tattaacttg ttcaaatgca ctcttgattc cttcaccacc tgaaggcaat ttatcccatt
    31921 cgatgtaagc acctttatct aattttacag ttgatacaac tggtaagaat ttgattgata
    31981 cccatctttg tacaagggca gctaatacga acatccctaa aattgaagca ccttttgtta
    32041 cgtcttgtaa caatccgcca gaaaggtcat cagtaatttt agaaccagct ttgtagccaa
    32101 attcttgtgt ataccacatg aatgcccaac gaataatgtt ccaacctaag aagaagatga
    32161 ttggtccaag gatatttcct cccatagcta atgaagcacc tagtgcacct agcattggac
    32221 gtactgtaaa ccagaacact gggtcaccaa caccagctaa aggacccatc atacctactt
    32281 taaccccttg aatcgctaca tcatcaacag gcgcgccatt cgcacgttct tcttccagag
    32341 ccaatgtaac ccctaaaatt ggagaagcaa tgtatgggtg tgtattaaag aattccaagt
    32401 gacgttttaa tgctgcagaa cgttcttctt ttgttttgta taattttttg attgctggaa
    32461 tcatagaaaa tgcccagcca ccgttttgca tacgttcgta gttccatgaa ccttgaatga
    32521 aggtagagcg ccacgcaaca gctaaacgat ctttttttga taatttgatt ttttcttctg
    32581 ccattttatt ttcctccttc aagattaata gtcattcaaa atgtcgccta aagggtcacc
    32641 agagttgctt ccgcctccat tatttgatga accacccatt ttagaaaggt tcaagtaaat
    32701 aagagctaat gcaacaccta aagcacctag agcaatcaat gttaattgag aaattgcagc
    32761 tacaacgaaa ccaataacga agaatggcca tacttctttt gtagccatca tgttgattac
    32821 caaagcgtaa cctacagcta cgaccattcc cccaccgata gccataccat ctgttaacca
    32881 tgctggcatt gattcaagga atgattgcac agtttgtgct gggataaata gcaacgctgc
    32941 tgctgggacc gcgatacgaa ccccttgtaa acatacagca atgatgtgcc acatttcaac
    33001 cttacggatg tttccttctt cagcagccgc atccatcaaa tgaacgattg gaacagcgat
    33061 tgtacgtacg atcattgtta agaacaaccc tgcaacagcc aaaggaacgg caatcgcgat
    33121 cgcagaagga acacctttaa caccttgtcc acctaaaact aaaataattg ctgatgctac
    33181 tgatgctaat gcagcatctg gtgctacagc ggcaccgata tttgcccaac ctaaagcgat
    33241 catctgaagc gtaccgccaa gtacgatacc agcttcgagg ttaccggtaa ctaaaccgat
    33301 caatgtacat gccactagtg gttgatgaaa ttgaaattca tcaagaatac cttccatacc
    33361 agctagaaag gcaactaata cgactaaaat tattgaaata atagacatga taagcctcct
    33421 ataaatttta atattattta ttacgctttt gctaattcag cttttgcttt tttcaaaata
    33481 tcatccatgt tatcgcgtga atcattaggt actttacgta catcgaattt cacacctttt
    33541 tgttcaagct tctcaaacgc ttcaacgtct tcttgtccca tagacaatac tttacttaca
    33601 acgactttac cgaccgaatg cgccattgac ccaacattta attctttgat gtctacgcca
    33661 ccttcaacag ctgtcaatac gtcttcagga ttttcaaata aaagcaatgc tttggtattt
    33721 ccaaaacgag ggtcttttgc tacttcgatc attttgctaa taggaataac atttgctttt
    33781 acacctggag gagcagcttg ttcgattagt ctcttacgca gatcatcctt tgatactgca
    33841 tctgaaacaa cgataatacg gtttggttgc actgcttttg tccaagctgt cgcaacttgt
    33901 ccatgtaaca gacgtgagtc aacacgtgct aatacatatt tgatttttcc gtctccgacc
    33961 acagttcctt ctggaagtgc tcctttaggt tgtgcgtctt caactgctgc tttcggtgct
    34021 tctgccggtt ctaattcttc cggttttact tttacaccgt cttttgctgt ttcaatgatg
    34081 tgtgctgcaa tttcatgagc agattccatt gaaaagcgtg atgcgtaggc ttcaatcaac
    34141 attggtaaat tcaaaccgct gacgattgcc catttatctt tgtgctcttc gaataatgtg
    34201 tttgattgat tgaacggtgt tccgccccat aaatcaacta agaataacac ttcgtcttga
    34261 ttatcaaaag aagcaaccgc ttcttccaat ttggctctca agtcatctgg accttcactt
    34321 ggtttcaaaa tcacggcttt gacgttttct tgttcgccaa agatcattga accggattgt
    34381 aagattcctt cagcaaattg tccatgactt gctaggataa tccctaccat atttatacct
    34441 cctatttact tttgttgtaa caaattcaaa aatattaata attgctaacc attacaacag
    34501 aattatttga ccttttccaa caaccattct tgctacacct ttttgatttt cgctagtagt
    34561 tccatcaaaa ggattttcct gtcagcaggt acttttcgaa tctctaactc cacttcttga
    34621 ctgttcaagt aacgaaagga atcaatatct tgttgattta ctgaaacaaa attggtaatc
    34681 attgatttgc cttcagtaaa actcataccg ccgatattca acgacttcaa ctgtacaccg
    34741 cagcggacaa ccgcttcaac atcttgggga ttggtgaata aaagcatcac ttttgctcgt
    34801 gataataatc cactatgata gatttgaatc attttctgca cggttattac atttgctgtg
    34861 atccccggcg gtgcagcttc ttttagtaga aactttctca atttatcttg agcgactaca
    34921 tcgctgatca caatgatacg ttcaacacct gtttgcttcg accaaactgt tgcgacttgc
    34981 ccgtggatta aacgatcatc tatacgtgcc aatcgaatat ccatctgcaa aactccttct
    35041 tcctcactac ttcacagaaa cccctatcat ttctgtgtga acaaccacct cctagcacac
    35101 actactataa gcaaaaagcg tgccaacttt tttacgatac actaattgaa tttaaaggat
    35161 tcttaaccaa tacacaaaaa cgatacacta taaaagtgta tcgtttttgt gtattcatgg
    35221 cacagtgtat tcttttttat ctaactgatt atggactaga cgtagcagat aatacatttc
    35281 ttctatcggc acttttactt ttatctgatc ttccaacaga acaactgagg ctgccaattg
    35341 gctataaaga ggatcattcg ttgtatttgt caattcgttt tcttccacag tcaatggttc
    35401 attccgaata atacgttcga tcattcctgc tgtgtgcatc actaggttga ttttgaatcc
    35461 atattcttca tcgccgattc ccgtcgtctg agagatttgt gtagaaaatt gccacattgg
    35521 atcgattaac ttactaccat taataaagat gaagttatca gagataaact caacacacgt
    35581 atttttagct gactcttcat ctagggagat gaattcagtt tcctccaatt ctgattccat
    35641 cagcaactga tccaaaatga cttcgatatt ttgatcaata aaacgttcta aaggaataaa
    35701 tggggcttgg atattcaaat cagtaatccc agtagtagct atgatctgat attctttttg
    35761 gataactggc agttcttctt tcacttccac aatcgacaaa gctagaactt ctaattctgt
    35821 ttcttgacgc ttgtttaccg ctcgttcgat caattctttg attctttgtg ctgtaccttc
    35881 gccagatgcg cagatagcca aaacagcttt tttcttatga ataggttttg cttcctgaac
    35941 agattctaca gaagatattt ctgcgtaacc tcgaaaattt ttcaatgatt catgcaacat
    36001 atctagatct gtccctaata ctgagacctt tcgtacagtt tccaaaacta gagaagttgt
    36061 taccatatca acagtacgta ctggaattcc agtgtctctt tgaatttgcg aattaaacga
    36121 cgcaagggac cccatatcta ctaagagtat gaccccgctt ccttcatcta cttcttgtac
    36181 attgcgttca attcggtcca atgctgtttt cggatccata tccaaaggca tgtctactgc
    36241 tcgtacgttc tctgcatcta acaactgttg gacaacttgg accatactgc ttgctgtgct
    36301 gtttccgtga gctgcaacga ctacaccgat tctgccgctt gcttgatttg cccgtaacga
    36361 gacgagtaga acggtcaagt aatagtcttc actttctgga atctccactt gaaaatacgt
    36421 ttcgatataa ctacgcactt ctttcgcaac agcatattca tctggaaaat caaaagccat
    36481 ctcacggatg ttatcattcg tatgccgttc ttctcctaag ttgatttttt tcaaaaaaga
    36541 actgatatgc aagctcatag cataaataaa gttttgctga aactcatatg gtaaagatct
    36601 tttgaccatt acataaatct gattggtcac ttctatgatt ttcggatcta caaattctgc
    36661 taatttgctg tctgcattaa aagaaaaacc atggtcttta tagaaagact tcagatggat
    36721 gttgatatct gtagaaatga aatgattgat tgcttcttga tccaaaccat ccgacttcag
    36781 taaagctgct ttatctccga tgatatcata tagattataa ggtaactcat aggagtccga
    36841 ttggattttc atgatcgtat catttggtgc tacagttatc tttggctcca atattttaga
    36901 aagctctgcc attgcactgc ggttgcttgc taattgaatc aatccgctac gaatcccttc
    36961 tgtcaaatca tcgattgtga tcgagatttc gggtgattgc atatgattta gaaatcctcg
    37021 tgcacatact aactggatat ttgacttcaa ttgtcctata tttccatagg tcacactgcc
    37081 gatcaatgct tttgctacat cttctgtcaa agaaatcttt cgctgcgtgc ggtttgcttc
    37141 gtgggcaatc atgattttta ctaaatcgac tttctcattt gcaggacgtt tttcaaaagc
    37201 cggaagtttg atattgattg ggatacgccg aacaaaagtt tctaatagag aagaacctgg
    37261 gtcttctgtg gtcgcaccta cgatccgtac atttgcttca tgggatttcg ttgtttcccc
    37321 aagacgactg taagttccat gatccataaa gtaaaagatc atttcttgac cttcaggcgg
    37381 caaccggtga atctcatcca aaaacagcat accgccatcc gcttgatcga taatccccat
    37441 tttttcttca tctgcgcctg taaaagcacc ttttgcgtac ccaaataaat gactcatcaa
    37501 aagctcaggg ttattggcat aatcagcaca attgaaaaca atcagttccg tttcctcatc
    37561 tattacatga tgcgcttttg caaaatgata catcgcgtgt gcaaaataag tctttcctga
    37621 tccggtaggt cctgtaatca aacaatttaa tcctcttggc ggatacaaaa tcgcagcttt
    37681 tgcctgttcc actgaatttt tcatgctgcc gttagctcca atgatttttg caaagatatc
    37741 cttagtatct atttggagtg tatttttttc ttcagagacc ggcgtttctt tgtaactgac
    37801 tacatgtttt gtaactggct ttacgtgcgt aacgagtgtt tgatatatgt aacgtactgg
    37861 tcgtccgtct gtctttatca gttttccttc gcgcactagc tgattcagat ctttactgct
    37921 atttgttcgc tgaatcccta acacttcagc aatttcctgt gttgttactc cttgatcata
    37981 ttctgccttt gttaaatgag cggttttttc tttgacatac atataaattc ggtcaatgcg
    38041 ttttgccaat ggtatcgctc cttttctata gactattgta cttaggcttc aaaacataat
    38101 actatgtatt tatttctgtt tcgtcaacta tttacctgat tttgttttgt tgataacacg
    38161 cggatagata gaaatgttca atttaaaaga acgaaaaatc ccgatacgat ttttataatg
    38221 gaaacaagaa aaagaacact gtttaccata taattatttc actgatagca aacaatatag
    38281 cttccatcaa ttgaaaacga tagttatcaa aacatcctga ttccaaattg gcaaaaaaat
    38341 attaagtgta aaaagacaaa gtaaacttcg tagcctccac tacaactttt atttttataa
    38401 acaaaaaagg ctgtgacaaa agtcttgtca caacctcttt aatcataaga ttcaattaag
    38461 cttcttgagc tactggataa acagacactt gttttttgtt acggccttta cgttcgaaac
    38521 gtactacgcc atcaactttt gcaaataaag tgtcgtctcc accgataccc acgtttacac
    38581 ctggatagat ttttgtaccg cgttgacggt aaagaattga tccacctgta actgtttgtc
    38641 catcagcacg tttagcacct agacgttttg attctgagtc acggccgtta gatgtagaac
    38701 cgccaccttt tttgtgggca aataattgta gattcataga taacaacatg gtctgcacct
    38761 cctatttatc attaattgtt ttaatttgga tatattctga gttttcagct tctatcgatt
    38821 gtaatcctaa taaaagattt tctaatagaa tctcagcaat attgttttgt tcttgtgtca
    38881 ttcctgaagt gacttcggta taaagataac cgccttcttc ttcattcatt tcaacaatcg
    38941 gctcaaaacc ggctaatgag gcaatcccgt tgacagtgct gatcgctaac gcagaaacac
    39001 ccgcacaaac gatatcactt ccataaggac cagcatcagc atgcccggtc aaagtgaatg
    39061 aaacgatctg acctgcgtca tttcgcttaa atgttccttt gatcatatat aggccttctt
    39121 tctcaaacgc tattaagcgt tgatcgcgtc gataaccact tttgtatatg gttgacgatg
    39181 accttgttta cggtgtgaat gttttttagg tttgtatttg aaagtaacga ctttcttttg
    39241 tttgccgtgt ttttcaacag taccttcaac agttgcacct gcaacagttg gagcgcctac
    39301 ttttgtagat tctccgccta ctaagataac ttcgtcaaaa acaacttttt ctcctgcttc
    39361 aacgtcaagt ttttcaacat agattgcttg tcccacttca acttttactt gtttaccacc
    39421 agttttaata attgcgtaca tgctataaag cacctccttc tgatatactt agactcgcca
    39481 tccgaagtga cagcatatgc tgtgcttatt acttgttctg agcggttgta gctgtggagt
    39541 ccacaattac aacagtacta gcttaccaga acagcaaccg ttagtcaact aaatttaaaa
    39601 tcaaaaagta aaacaaaaat tcaagcaata cttctgcttt tctatcctca cagttctacc
    39661 aaataaaata gtcgcattgt gtttctttta ttcttataat aaaagaaaaa ggaggagcat
    39721 aaatggaata cccaaaagaa ctgatacaag aagcttcttt aagaatgaac ggccgtcctg
    39781 tagaaggatt tatcgctggg caaggacctc ttcatcctaa actgatgctc gtgggagaag
    39841 ccccagggaa aacagagatt gatacgcatg ttccttttag cggtcaagct ggaaaagaat
    39901 tgatgcaggc actttcttca actggcttaa cacgagaaga ggtgtatatc accagtgctg
    39961 taaggagccg cccgtatcgc gtgactcatc ggatcaataa acggacacaa cagtcagaaa
    40021 ctgtttatcc aaatcggaca cctactcgtt ccgaagtatt tgcgcatgca ccaatattgg
    40081 attacgaact gatgaaggtc cagccaaaat taattgcgac acttggaaac attggtcttc
    40141 aacgtttact tggcaaagaa tatacggtta ccaaaaatca tggtcaactc tatacagggc
    40201 ctgtcttaca attaacagaa caaaaggatg cctacgaatg gtcagaaaaa aactatcgaa
    40261 ttcttccact ttttcatcca gcagctattt tctacaatcg ctcccttgta cctgttatcc
    40321 atcaagactg gcaagtacta gcttcacttt tacagcaata ttaaaaagaa cagacaagac
    40381 cataaaatct tcagtttaca gtcttgtctg ttctttttca ttgttttaat ggctaattgc
    40441 agtatatttc gcatgtaaag ctgtcaacgc atgagcaact gtttgatgtt gatgcgtgaa
    40501 tgtatgttcc cacaagtgag aaagttgttt ttctacttct ggctgcaagg aagtatagat
    40561 aatcgtttgt cctctttgct ctgctttttc ttgaaattca tcaaaaactg cacgtgccgt
    40621 caaatcaata cttggtacgc ctcgcatgga gaaaatgaca ccatcgtgat gatttagttc
    40681 ttccaattta tttttgagtt tttctgaaga cataaaaaac agtggaccac taatatacac
    40741 tactgtccag ttattcagct tttccgtttc tggcagcccc attctttgcc aatcgatcgt
    40801 ttctaatgaa atcgtgatag cagcgctttt gacaacaaaa aatacacagc cgccgatcac
    40861 cccgatcacg atagcaatac ttaaatcaaa gataactgta caaatcatcg taaggataaa
    40921 caatagaact gctgaccaat atttattagt aaatagctcc ttgatcgttt cccattcatt
    40981 catacgccaa gccgtcacaa tcaaaacacc tgcaagtgct ggcattggaa tctgtgacat
    41041 cactggcgca aaaacaagca tggagagcag taagacaact gcatgtatca taccagctaa
    41101 tctagtttgt gcaccagatt tgatggccac gcttgtacgg gcgatggccg cagtagcagg
    41161 aatcccgcca aagaaaggaa gcaacagatt tccaattcct tgcgccatta attcttgatt
    41221 gctatctaag ggcttatttg ccattcggcc agccgaagca ccacatagca agctttcaat
    41281 cattcctaat aaagcaatgc taatagctgg aactgccact tgttgcaagg cagaaaagct
    41341 aaaatctccc atattcaaac gattactact gatcaatgtt tgtggaatct tacccacagt
    41401 tgctactggt aaatggaaca atagcataac tgctgttgcc aaaatgatag ccagcagcga
    41461 actaggcatt cgtttcgccc atttttttgg atagataaac atccctaaaa tgactagccc
    41521 tcccatgatc aaagaggctg tgtttatcgg aaatcctatc gtttgataac tacttagttt
    41581 ttcaataacg tttgtacctt ccgagtgaac accaaataaa ttatcgatct gacctaatgc
    41641 aataatgata gcaatacctg aagtaaaccc cgtaattacg ggtgctggga taaaagaagt
    41701 cagtgttcct aatcggaaaa gtcctgctgc aagtaaaaat agacccgcta aaaaagtagc
    41761 aagcaataca ccttgcattc cctttgttgc cgcaatagac atcaagatag ctgccatcgc
    41821 acctgtcgga cctgatattt gataaaaacc gcctgataaa ccgccaataa cgataccggc
    41881 aatgatagcc gtaatcaaac ctgcggccgc atcagctccg cttgataccc caaaagccaa
    41941 ggctaaaggc aatgctacag cagctactgt gatcccagcc aaaagatctt tctgaaatct
    42001 tgaaagatca taacctgaaa attctttttt tagtgaggaa acataatttt tgaacaccta
    42061 tctaactcct ttttcatgaa attttctaaa ataccattca ttagaatatc atatttcatg
    42121 agataaagta atatttttca caaagtttcc tgttagataa attcttattt tttctatatt
    42181 tataagaaaa tagaaagcaa tgccaacaga gctagcccac cttgtttgag taaaatccaa
    42241 cgatcgctag tcaaactgcc atataatgca acaaaaaaaa tagatattaa tagcatactt
    42301 gtgatttctt tagatgcgga agaaagataa gctccataaa gtagtcccac tcctaatagt
    42361 ccattgtaca ctccttggtt cttaaacaat gtttgaacag attttctgct taactcttct
    42421 ttactcattt taaaaaccct acttgtcgtg tcagaatcag ttctcaccgt ttcgatatac
    42481 atgatataaa aaaattcaat tgcaactaga gtcactaaaa ttctagatag tatcgtcata
    42541 tcttcttcct cttttctgat gagttttatt tttttgctcc ttataattgt actcgataag
    42601 gatgtttttt tattttattt gcctttgaaa acaaacaata ttggcacaaa aaacaaagaa
    42661 aaaccgttag aaacctcagt ttctaacggt tttttagcgt cacaggaggg attcgaaccc
    42721 ccgaccgtcc gcttagaagg cggatgctct atccagctga gctactatga caagaatcag
    42781 cggaacatca ataattatag ataattaaat tgcttctgtc aatacgaaat agttatttaa
    42841 ttagcaaata atgaattatt ttggcccaac tactaaatca atcttttaaa acgagacatt
    42901 ctccttcagc tgttctaagg ttatcaattg aaaacaggag ctacattgac ttcatgctac
    42961 tcctgtttat ttagatttcc tgtatccttt aattgactta caaatataga aacttttaca
    43021 tcaagttcga attgagccct cgattcaatc gattatctac aggctgccat aatcatgtat
    43081 cacaactcga ttgtttctga ttcctttgtc attaagatca caccatcacc taatggcaag
    43141 attgtcgatg tcaaatcggg atgatccatc acaacacgaa gaaattcatt taatttacga
    43201 tggatagctc gtttgccacg gggaatctct tcatctggga gcaagatcgt tcctccttgg
    43261 aagatgtcat ctaccatcaa aacgccacct tttcttaaca aacgtaagca ttcaggtaaa
    43321 aattcaatat attttgattt tgcactatcc ataaaaataa agtcatacgg accagacaac
    43381 tccggcaaga tatctgctgc ttgaccttca agcaaagtca ctttatctgt cagtcctaga
    43441 cgttcatagt tttttttagc tttctcgatc atgacatcga aacgatcaat tgtcgtaaca
    43501 tgagcgtttt ctccgattgt cgtggccatg acacttgatg aaaaaccgat agctgctccg
    43561 atctccaaga ttcgttcagg ctgaagctgt ccaagtaaaa atttcaaaaa tacaactgtt
    43621 tcatggggaa taactggtac ttctgctaca tgagcttctt cttccagttt gcctagctca
    43681 cccggtaatt ttttttgctg ggtacgcata aaatccaata gttcttcttt gactacagga
    43741 cgatgcatca tttcattgcg catactactc ctcctcttca cacattatac gaacaactga
    43801 ctaaatctac aagctgcatg agtgttttct cgtgtttcat gttcagaaga gtcgttggat
    43861 ctctggaata catttatgtt tttaacattc ttacaaaaat ttctgtgttc gtctccgctt
    43921 ctttttcaac tctacaaagg tattccatgc tgtgattcct gttaggcaaa aagcaatcgt
    43981 aaagaataac ccctgaatcc atgcaaatgc cgtttttgaa aggactagtc caatatatgg
    44041 aatctgaaag atgggactac cgataatatt tctctttttt actactccag gctcaatata
    44101 agtctttgta tcagcttttg tataaaaatc ttcgtggttg ttttcaacgg ctattacgcg
    44161 acgcgtcgag attttttttc ctgaattttc ataatatgta ataatatctc cgactaaaat
    44221 ctcatttgta tttttcggtc gtacatatat cagatttcct tgtgcataat atggactcat
    44281 ttctttgtcc tcaataatat aaggagtcaa accaaaaatt cggggaacca agacgacgag
    44341 aagaataaga attaaaaata taatcaacgt tatagaactt ataaaagtta tttttttgta
    44401 atcaggtatc gttttcaact tctttcttta ttcaatcctt cttaatcata taataacgat
    44461 aaattttttt caaattatag ctgtaaaaat gtttcccttt aataattata tgtttctaat
    44521 tctatttcct catctttatc tcatttttta ttataaataa ttgacgttag tttaaaaaac
    44581 gtttaccc
//
LOCUS       DACDCV010000012        42904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDCV010000012 DACDCV010000000
VERSION     DACDCV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.012"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV041_000687"
     CDS             591..1280
                     /locus_tag="IV041_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8381503.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV041_000688"
     CDS             1267..2475
                     /locus_tag="IV041_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8381504.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV041_000689"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV041_000689"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8381505.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV041_000690"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV041_000690"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8381506.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV041_000691"
     CDS             complement(6578..7273)
                     /locus_tag="IV041_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8381507.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV041_000692"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV041_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8381508.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV041_000693"
     CDS             complement(8472..8663)
                     /locus_tag="IV041_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8381509.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV041_000694"
     CDS             complement(8684..9574)
                     /locus_tag="IV041_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8381510.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV041_000695"
     CDS             complement(9561..10328)
                     /locus_tag="IV041_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8381511.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV041_000696"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV041_000696"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8381512.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV041_000697"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV041_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8381513.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV041_000698"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV041_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8381514.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV041_000699"
     CDS             14944..15282
                     /locus_tag="IV041_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381515.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV041_000700"
     CDS             complement(15450..16208)
                     /locus_tag="IV041_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8381516.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV041_000701"
     CDS             complement(16226..17044)
                     /locus_tag="IV041_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8381517.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV041_000702"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV041_000702"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8381518.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV041_000703"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV041_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8381519.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV041_000704"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV041_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8381520.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV041_000705"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV041_000705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8381521.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV041_000706"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV041_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8381522.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV041_000707"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV041_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8381523.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV041_000708"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV041_000708"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8381524.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV041_000709"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV041_000709"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8381525.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV041_000710"
     CDS             27086..27385
                     /locus_tag="IV041_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8381526.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV041_000711"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV041_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8381527.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV041_000712"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV041_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8381528.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV041_000713"
     CDS             28352..30325
                     /locus_tag="IV041_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8381529.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV041_000714"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV041_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8381530.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV041_000715"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV041_000715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8381531.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV041_000716"
     CDS             33204..34496
                     /locus_tag="IV041_000716"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8381532.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IV041_000717"
     CDS             35232..36095
                     /locus_tag="IV041_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8381533.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IV041_000718"
     CDS             complement(36224..37846)
                     /locus_tag="IV041_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381534.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IV041_000719"
     CDS             38095..38616
                     /locus_tag="IV041_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381535.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV041_000720"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV041_000720"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8381536.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IV041_000721"
     CDS             40697..41515
                     /locus_tag="IV041_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8381537.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IV041_000722"
     CDS             complement(41618..41815)
                     /locus_tag="IV041_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381538.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IV041_000723"
     CDS             41984..42562
                     /locus_tag="IV041_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8381539.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13023 a         8233 c         8474 g        13174 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaag
//
LOCUS       DACDCV010000013        40870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDCV010000013 DACDCV010000000
VERSION     DACDCV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.013"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..391
                     /locus_tag="IV041_000724"
                     /pseudo
     CDS             123..391
                     /locus_tag="IV041_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1063..4464
                     /locus_tag="IV041_000725"
     CDS             1063..4464
                     /locus_tag="IV041_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8381540.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(4888..5058)
                     /locus_tag="IV041_000726"
     CDS             complement(4888..5058)
                     /locus_tag="IV041_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8381541.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5252..5431)
                     /locus_tag="IV041_000727"
     CDS             complement(5252..5431)
                     /locus_tag="IV041_000727"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381542.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5818..6930
                     /locus_tag="IV041_000728"
     CDS             5818..6930
                     /locus_tag="IV041_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8381543.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7008..7301)
                     /locus_tag="IV041_000729"
     CDS             complement(7008..7301)
                     /locus_tag="IV041_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8381544.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7469..7783
                     /locus_tag="IV041_000730"
     CDS             7469..7783
                     /locus_tag="IV041_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8381545.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7804..9783
                     /locus_tag="IV041_000731"
     CDS             7804..9783
                     /locus_tag="IV041_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8381546.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9796..10122
                     /locus_tag="IV041_000732"
     CDS             9796..10122
                     /locus_tag="IV041_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8381547.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10138..10656
                     /locus_tag="IV041_000733"
     CDS             10138..10656
                     /locus_tag="IV041_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381548.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10656..12008
                     /gene="celB"
                     /locus_tag="IV041_000734"
     CDS             10656..12008
                     /gene="celB"
                     /locus_tag="IV041_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8381549.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12092..13597)
                     /locus_tag="IV041_000735"
     CDS             complement(12092..13597)
                     /locus_tag="IV041_000735"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8381550.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13689..14279)
                     /locus_tag="IV041_000736"
     CDS             complement(13689..14279)
                     /locus_tag="IV041_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8381551.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14458..16104
                     /locus_tag="IV041_000737"
     CDS             14458..16104
                     /locus_tag="IV041_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8381552.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16153..16872
                     /locus_tag="IV041_000738"
     CDS             16153..16872
                     /locus_tag="IV041_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8381553.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17351..18298
                     /locus_tag="IV041_000739"
     CDS             17351..18298
                     /locus_tag="IV041_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381554.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18310..19230
                     /locus_tag="IV041_000740"
     CDS             18310..19230
                     /locus_tag="IV041_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8381555.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19271..20728
                     /locus_tag="IV041_000741"
     CDS             19271..20728
                     /locus_tag="IV041_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8381556.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21073..21687
                     /locus_tag="IV041_000742"
     CDS             21073..21687
                     /locus_tag="IV041_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8381557.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21680..23404
                     /locus_tag="IV041_000743"
     CDS             21680..23404
                     /locus_tag="IV041_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8381558.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23401..24852
                     /locus_tag="IV041_000744"
     CDS             23401..24852
                     /locus_tag="IV041_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8381559.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25325..27478)
                     /locus_tag="IV041_000745"
     CDS             complement(25325..27478)
                     /locus_tag="IV041_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8381560.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27480..28526)
                     /locus_tag="IV041_000746"
     CDS             complement(27480..28526)
                     /locus_tag="IV041_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8381561.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28676..29965
                     /locus_tag="IV041_000747"
     CDS             28676..29965
                     /locus_tag="IV041_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8381562.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            29978..32680
                     /locus_tag="IV041_000748"
     CDS             29978..32680
                     /locus_tag="IV041_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8381563.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32665..33522
                     /locus_tag="IV041_000749"
     CDS             32665..33522
                     /locus_tag="IV041_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8381564.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33593..34450)
                     /locus_tag="IV041_000750"
     CDS             complement(33593..34450)
                     /locus_tag="IV041_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8381565.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34661..36832
                     /locus_tag="IV041_000751"
     CDS             34661..36832
                     /locus_tag="IV041_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8381566.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            36900..37241
                     /locus_tag="IV041_000752"
     CDS             36900..37241
                     /locus_tag="IV041_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8381567.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37517..39271)
                     /locus_tag="IV041_000753"
     CDS             complement(37517..39271)
                     /locus_tag="IV041_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381568.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39282..40118)
                     /locus_tag="IV041_000754"
     CDS             complement(39282..40118)
                     /locus_tag="IV041_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8381569.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(40120..40803)
                     /locus_tag="IV041_000755"
     CDS             complement(40120..40803)
                     /locus_tag="IV041_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8381570.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        13256 a         7146 c         8468 g        12000 t
ORIGIN      
        1 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
       61 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
      121 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
      181 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
      241 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
      301 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
      361 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
      421 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
      481 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
      541 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
      601 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
      661 tgaaggttaa ccaatacagg gattcactga tgaaattcta gagtctgacc ttggtatcaa
      721 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
      781 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
      841 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
      901 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
      961 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
     1021 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
     1081 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
     1141 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
     1201 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
     1261 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
     1321 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
     1381 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
     1441 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
     1501 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
     1561 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
     1621 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
     1681 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
     1741 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
     1801 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
     1861 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
     1921 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
     1981 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
     2041 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
     2101 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
     2161 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
     2221 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
     2281 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
     2341 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
     2401 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
     2461 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
     2521 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
     2581 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
     2641 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
     2701 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
     2761 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
     2821 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
     2881 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
     2941 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
     3001 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
     3061 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
     3121 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
     3181 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
     3241 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggtttaact
     3301 acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata tcagctggta
     3361 gaaactcaag cgccaatagg gtatgaatta gatgcgacac caatcgagtt tgagatcgaa
     3421 agaagtcaga cagcggtggt agaattgacg aaagaaaacc gacttacacc aggcggtgtc
     3481 gtgttgacaa agatcgatga ccaaagcggg gaaatcctgc aaggagctgt ttttgagtta
     3541 cagaaccgag aaggagaaac gctacaaacc ggtctaacta caggagaaga tggaaaactg
     3601 gcaattgacg gtttagcacc aggagcatat cagctggtag aaactcaagc gccaacgggg
     3661 tatgaattag atgcgacacc gatcgagttt gagatcgaaa gaagtcagac agcggtggta
     3721 gaattgacga aagaaaaccg acttacacca ggcggtgtcg tgttgacaaa gatcgatgac
     3781 caaagcgggg aagtcctgca aggagctgtt tttgagttac aagatgccaa tggaaccgtt
     3841 ctgcagtcag gtctaaccac aggagcagat ggaaaactgg cgattgacgg cttagcacca
     3901 ggagtgtatc agctggtaga aacccaagcg ccaacggggt atgaattgga tgcgacacca
     3961 atcgagtttg agatcgaaag aagtcagaca gcggtggtag aattgacgaa agaaaatcga
     4021 cttacaccag gcggtgtcgt gttgacaaag atcgatgacc aaagcggaga agtcctgcaa
     4081 ggagctgttt ttgagttaca agatgccaat ggaaccgttc tgcagtcagg tctaaccaca
     4141 ggagcagatg gaaaactggc gatagatggc ttagcaccag gagcatatca gctggtagaa
     4201 actcaagcgc caacggggta tgaattggat gcgacaccag taactttcaa gatcgaaaaa
     4261 gaacaagaag tggcgatctt tttaacaaaa gagaatagaa aagttgctga tagctcagat
     4321 ataagaggaa acaatccaac aagcagtgga aataagcatc ttccaaaaac tggagaaacc
     4381 atcattagcc agtttatatt gtcaatattg ggtgtgctac ttgttttcat cagtataaaa
     4441 tttagaaaga aacgaaatat atgagctaaa ataaaaaatt gaataagcat agtttttaaa
     4501 atcaactagt acccatcaat tgaatggaaa ctaccgattt tagaaggggt atacaaacaa
     4561 atattagtat cggagtagag aaggttgaac ccaaccagta aataagtaaa gacaattttg
     4621 tgctgcgttg agaagtgaaa aataaacctt tgtagtaata ctagttaata aaaaatatac
     4681 aaaaacactg cgaaaatcat tcttgagttt cgcagtgttt tttgcctaat gactcaagtt
     4741 tattgggtaa tactttgatt ttctttctac aaataatcag cgatcagttg taattttttc
     4801 tctagtttta atggctcttt gtcaataagg agaaaaacaa gtcgggtctt ttgtctcgga
     4861 aagcttcaac caactcgtga ataaaggtta cgcctccttg aaaggcgtgg aaaaaccaag
     4921 taatcgatca atcaccaatt tcgcatttgg tagaacatgt ttggtgagct gaaagtaggt
     4981 ggcgttcatg tctgtaacta agaattctat ttctttagga ttagtgcagc taaggaaata
     5041 gtttgttaat cgaggaattt tttgtgtagg taaattttct cgattaattt ttctgtctcg
     5101 ccatccgcgg aaataaaact cagtttatcc tcaacggaaa cgtgagaacg aaattcacaa
     5161 ccctcaacac tcaagacaga attcttttgg aagactttgg taaatagctt ctcaactctt
     5221 tcaatgtgcg aataacggtt gtcaaagata cctaacaact tttcgaaata aaaaataacg
     5281 acactttttc agtcagtaaa gaagtgattt tatgtctgac atgatttgaa atcgagtgcc
     5341 ttggacggat aaaataactt tgagcgttcc aatggcttcg acagttttta caggtatagc
     5401 gctgcttttt taggcgtata accattggca tatgattata ttattcaaag tggacaatgg
     5461 tttccttctt cttgtttttc accaccatta atttcctatt tccatctacc acagcagaac
     5521 cacaatttct acagacacat ggagcaggga agagaactgc agcaacgatc aatgtttttt
     5581 tcttctgtaa ggtttcgtaa gagacctctg taatcatcaa atttttgtct attgatctca
     5641 gaattttttt gatagaacca ttcataaagc atatcgttct ctcagttgtt tattttgtgg
     5701 tgatttaatc atacaagaaa acgatatgtt tttttataaa aacatgaata ggaaaaatag
     5761 ttgacaagaa aatgacaacg ccatatgatg ttccatagta tttacaacct gtcatagatg
     5821 actgtaaaaa ataaaagtaa aaagaaagga cgacaacagg tggattttta tcagtctcta
     5881 caactagatc cgtttatttt aaaacaaaaa atacgagaag cagcttctaa aaaagaaaag
     5941 tgcatgtata tttgttcatt gtttttacga tcattattca tcgtattgtt tgccatttgt
     6001 tttatcatta taattacaac attatttgaa tcgaagcata aaccatacgc tgtggtttta
     6061 ttctgtatgt tgatgagtat tcgttttgtt gactttggtt ataagatttc ccattcgatc
     6121 ataggtttag caatcgtcat gttctcacta ttagtagcac cgtatgtcca gctgatcaaa
     6181 tggtcagtta tgggcatgtt gattcatttt attttactaa gcagcatatt aatggcaacc
     6241 gctagtgatc ccaaaatggg gaatgcttct ttgtacggct tttcatatct ctttattgtt
     6301 tattcgttac ctaaagattc gttgaacaag aatttcttta cacaaacaag tagcttatta
     6361 tttttgtttt tttgttggtt ttcagttctc ctttacagaa aacatcgaga aaagaacaaa
     6421 gacaaaagct tgtttaaaga gatttttttg aaaggcatgt attcgcagga aaaaatttgg
     6481 atgctcatct atgcatttgg cattagtctt ttgatcgttg caggagagta tgtccctttt
     6541 cagcgattga tgtgggcagg gtttgcattt tcttctattg tatctagcta tggattgatg
     6601 tcgattggtt tcaaagaacg agcagtagac cgaatcattg gctcattgat tggatgtgtt
     6661 ttatttattg gtatcagcca atttattcct tttaactggg tcggtatatt aggtggtcta
     6721 gctttaggtg tttgttctac ctatagatat aaaacagttt ttaattcttt tggtgctttg
     6781 gcaatcactg ccagtttatt cggggttcca ggagcagtaa cgatccgcat ttttgagaat
     6841 atccttgggg tatgtttagg aatcatgtat attggggtga ctgaaatact tatccgaaaa
     6901 ataagagaaa aacacggttt gaatcattag atgagatcga ataaaaaata aggaaaaagt
     6961 gaagatacac atggatgata aattgctatc attccatgtg tatcttttta cggtaaatca
     7021 gtgaaacgtt cgactttcat ttttaattta tactttttac ggagttcgtt tatttgagcc
     7081 atcatttcgg atttgtgatg cttttcgatt gcttcttcat ctttccaacg gtcgatcagt
     7141 aaaagagatt ctggatcttc tagtggaaag taatagtcat atttctcgtt tccttcttct
     7201 gcacgaatcg ctttgacaat gtttctttcg accatttctt ctgcaaattt tctggcttgt
     7261 ccatttgtac ttgtatacaa aatattcact gtgatactca ctaaatcccc tcctgaaaaa
     7321 attctatcac tagtatatca cggtgccaga aattattatt caagatatga tacacatttt
     7381 ttccgcaatg ctacggaaat atatattttg aatagaaaac gcttacaaga tatgcttggt
     7441 ttataaaaat aaaaggagtg attgatttat gaaaaaagca ttgattattt gtgcggcagg
     7501 aatgtcatcc tcgatgatgg cctcaaagac aacggatttc tttaagcaaa aaagagaaga
     7561 ggtactcgta gatgcagttt cagctacaga aggcgataat atgattaaga atagtgattt
     7621 tgatttattt ctgatcagcc cgcaaacgac gatgtttttg gataaattca aaaaattagg
     7681 agaatctgtt ggaaagcctg ttgttagtat ccctttccaa gcgtatgtcc caatccctac
     7741 aggcatccag aagttagcag aggtgatcga agagaacata tgatggaata ggagtggatg
     7801 ataatgaaac agaaggaaaa attgctgttg agatatttga ttgatcacaa gaaggagtat
     7861 gtcaccagtc aacgtttagc ttctgaattg ttattatctg atcggacaat ccgtaattac
     7921 ttgcagcgga tcaaagagtt agtagaaaaa aacggaggta agatcattgc taaaccaggt
     7981 tatggctacc aactgcatat tttacaaaga ctgacatttg acttgttttt gagtcggcaa
     8041 gaaatagtac ctggatatgc tagagaaccg caaggatttt atgagtccga agatcgacaa
     8101 aagtatatat tgaaccaatt gcttttggaa gatgccattc tttttatgga tgatttggca
     8161 gaagagcttt atatcagtcg ttccagtttg acaaaggaca tgcaagaaat caaggagcga
     8221 ttgattccat attcactaaa aatcgtttct aagcatggtc aagggacatg gatcgaaggg
     8281 gaagaaagaa atcgacggca ttttatcatg gatacttttt ttggaaacca ttatggaaat
     8341 tcattgaaag aatatttagg gcaaagccgt tttttcccag acatcagttt tgaagagcta
     8401 gtcattatca ttctcgatga aacaagagaa gctaagttaa aagtttcgga ttttatcata
     8461 cagaatctga ctttacactt ggctttgggg atcaaacggc tccgagaagg gtttgaaatc
     8521 aaggaattag gcatcgaaca agaagtttct aagcgagtag agtatcaagt ggcgcagcgg
     8581 atcgttcaac gtattgaggt aatcgcaggt gtatcgtttc ccgaagaaga aattgcttat
     8641 ttgacattac atctcatggc taagtcgaat cacaaagaaa atcaaaatga tcaggaacta
     8701 tcaaaggaat tagcgaaagt tctgaaaaga ttgtctcagg aatttcattt ttcattagta
     8761 gaggatctcc agcttttcaa tggactactc gatcatttgt caccgatgat gattcgattg
     8821 agccgaggta ttcgcattga gaacccattg actgaggaga taaagaaaga aaatcccaaa
     8881 gtctttgacg cagtaaaaag gcatttcagc aacatgccgg ctttgaaaaa ctataccatc
     8941 aatgaagatg aatgggccta tttggctttg catctcatgg cggcattgga aaaagaaaga
     9001 gctgcgcata aactacatgc attgattatc tgtgcaacgg gttacggcag tgcccaacta
     9061 ttaaagaatc gcgtagtcag tgaattcggt aaaaatatta cagtggtaag tgtaaaaggc
     9121 tattatgaga tcaatgaaac aactttaaaa ggaatcgacc tgattatttc ttctattgat
     9181 ctttcgacaa tgtttttcaa aattccagtt ttacatgtca gtattttttt gaatgaagat
     9241 gatgttcgta aaatacgaaa ggtagttgga gaatctatac cctatcattc ctcagataga
     9301 ccgataccag cattttctct acagcaaaaa aagcagattt acaccgaaca gttgtctgaa
     9361 aaatttttca agacttatac gtacgaaccg aaaaaggaag aaattcttca tgatttgctg
     9421 caaaagctga gcataaatga agaagaagga tatataagag aaatgcgaaa gcagatccag
     9481 caacgtgaaa atatgggaca aatcatattc agtgatacag tggcagtccc acatcccgct
     9541 gttccagttg gaatcgaaac aaaggtagcc gttgcattga ttccttctgg gatgcagtgg
     9601 gatcaatatc aagaaattca ttttgttttt ttagtttcgc cttcttatgt cgaaaatgaa
     9661 ggaattacgg tcgtgactaa agcaatcgtt aggttagttg accagctaaa agtacagcag
     9721 gaaatcttag ctgaacctat ttttgagaat ttcagcagta aatttatcaa actgatcgaa
     9781 taaggaggaa aacagatggc tgaaaaaata aacagtgaaa cactacaagt gacttctttt
     9841 gatatcattt tccatagcgg aaatgcacgg acactgatac atgaagcatt tcgctcgatg
     9901 cgtaaagaga tgtttgaaga agtagaagag aagttgcgac aagcaaatga agagattctt
     9961 catgcgcatc agtcacaaac tcaacttctg aaagaatatg ccagcgggac aaaaatagaa
    10021 atggaaatta tcatggtcca tgcacaagat catctgatga cgacgatgac attattggaa
    10081 gttgcacagg aaatgaatta tttgtatcaa aaaaataaat agaaaggaag gatcattgtg
    10141 caggatacag taaaaaggaa aatcgaatta gaaaaaaagc aacttagttt gaaaaacatg
    10201 tatttcaatc gttatctaat cgtacgttac ctgactgcct ttttcttttt catgaacatg
    10261 caatggatga ttctgcttct atcagctaaa agtttaggaa gtagtttacc gatgattctg
    10321 attttagcta ttttgcctgc agtaggagaa caagtaaagc tttatcgaaa acaccaaacg
    10381 aacgttccat ggacaaagtg gtattttttg ttccagagtg tctttaattt attagtgatt
    10441 ccagtgttgt ttacctcagg atttacattc ttgtatcctt ttatggcaaa taacaatgga
    10501 ggacagttat ttgttttcat tcttgttgta agcggtattt ttgccagtgc atttatacag
    10561 tatcgtttaa agaaaatttc actcaaccaa gaccaacagt atatccgtat taaacaatat
    10621 gaaaaagcgt tatatttagg aaaggagaat aactaatgtt tgattttttg cagaaatacc
    10681 tgatgggacc tatggggaaa attgcgcaac ttaaaatcgt tcgggctgtc atggcagcgg
    10741 ggatggcttc tattcctttc acgattgtag gctcgatgtt tttggtttta aatattttgc
    10801 cattaccgtt cccattttta gaaggatttt ttaatgcaac ttttttcaaa gttagtgact
    10861 tgtacatgat cgtcaataca atgacgatgg ggattttatc tatttacttt gccattgtgt
    10921 ttgcttatga attaacgtca attgaacgag atgaacaaaa attaaatgtt aatccgctca
    10981 cgggggcatt actatctgtt tttgcattct ttatgtgtat ccctgaattg atcatgactg
    11041 atggtaaaat cagcttgatt gcttctatca atgagacaga aactgtagta aatggtatta
    11101 ggatgggctc ttttgtcgaa cgtttaggaa cttcaggaat ctttacagct attattatgt
    11161 cgattattgc ggtcgaattg tattgtatgt gtgtcaagag aaattgggtc ataaaaatgc
    11221 cagatgttgt tccgccagga gtttctcgtt ctttcactgc tctgatccca acatttgtca
    11281 ttgcgtttgt ggtgatgatc atcaatggat tattagttgc actaggaacg gatatcttca
    11341 aaatgattgc tatcccattt gggtttgtta cgcagttgac gaatacatgg cttggtgtca
    11401 tggtgattta tttcttgatc catgcattgt ggatcgtagg gatccatgga gcgaatatca
    11461 tcacttcttt cttgaccccg atcgtattag ctaatatggc agctaacgca caaggcgcaa
    11521 attatccgtt ggcaggagaa ttcaacaatt cttatgtaac cgttggtgga tcaggtgcga
    11581 ctttaggatt gatcatcttt attgcattcc tcgcgaaatc tgatcagctg aaagtgttag
    11641 gaaaagcctc acttgtacca ggattattca atatcaatga accaatcatt ttcgggatgc
    11701 caatcgtcta caatccctat ttagcggttc cgtttttctt agctccgatg gcttctgcta
    11761 cactcgctta ttttgccatt cggctggaaa tcgtcaaacc gatgcttgca cagatgccgt
    11821 ggccttcacc agtcgggatc ggagcatttg ttggtagtgg tggtgattgg aaagctgctg
    11881 tgttagcagt catttgtgca atcatcgcat tcattatctg gttcccattt atcaaattct
    11941 atgataataa attattaaat gaagaaagag aaaaggcagc agaattagct agtgaaaagt
    12001 taaaatgatt tctaagaaaa aaggaactgt gacaaaaaat cacagtccct ttttttctta
    12061 gaaatgggaa accactagta tataaatacc attattcttc aatcaaccgc ttcacgattg
    12121 cataatcccc ttcataaggt tcatctacac cattgatttt ttgataggga tccacgcctt
    12181 ttcccgcaat aacgactgaa tcttgcggag cgctttctgc taaagcgcgt cgaatcgctt
    12241 caggacgatc catttcataa tgaacgatca aatctggatt agtaattgct tcattgattt
    12301 cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc tgccgtcaaa aaggcgatgt
    12361 cagctgtttc tgatagcact tttccaaaat catgtcgacg ggagattgct ttgtttcctg
    12421 ggctgcctaa tacaacaatt acacggccat ctggatgttc atttttggca aatttcagca
    12481 atgttttcaa gcttagataa ttatgtgcat agtccacata gacatgcgct ccattggatt
    12541 ggatcaattg atccattcgg cctggtacac gagcttcttt taatccgtct tgtgcatctt
    12601 gactggaggc acctgttagt gctgcggcaa tgatcgcact ggctgcattt tcttggttga
    12661 atcctccagc cagcaaaata tcataagcag tatcgcagat agctaagcga tcttcatgag
    12721 aagataaagt aaaaccgtga gtacctttat ctgaacggat cacttgataa ttgctttctt
    12781 ccgaacgacc ataagtgatc gttggaatct ggaatagatc ggctgtttcc ctaagtaaat
    12841 caaaatagtc actttcatga tttaaaacaa ctgtttttga attcaagatc aattgtcgct
    12901 tgcaatagaa atagtcatca aatgtcggat gttcgatcgg actgatatgg tcaggcgaga
    12961 tattcaagaa gattccgaca tctaaagtca agccgtatac gcgttgtgtt ttgtaagctt
    13021 gtgaagaaac ttccatcact agatgagtca taccattttc aactgcttct gacatcatac
    13081 gatataaatc taatgactca ggcgttgtca aatgggattt gaaatatgtt ttgccatcta
    13141 acgttgtatt catagtagaa agcaatgctg tttttttatt tgttgtgtga tccaagattg
    13201 cttttgtaaa gtacgcagca gttgttttac cttttgttcc agtgaatccg atcactttca
    13261 atttattttg cggatagtcg taaaaagcca tagcaagaag cgccattgct ttacggatat
    13321 ccgttacgat gattcctact gctgaatttt cgtatggttg ttctgataca taatattgaa
    13381 taccctgttc gattgctgaa tccaaatatt cttctttgaa attcattcct ttacagaaaa
    13441 acaaagtaga agcatcagct tttcttgaat cataacttaa ctgaagaaat tcagcttttt
    13501 ctggtacatc aagatgccag cctcttgttg aaacaaattc ttttaataga ttttctttta
    13561 atagtaaatg acggatgtct tctaaagaga agctcattga ttgatccctc gctttattca
    13621 atttcttggt ttattatagc acactctttt taatatatcg tttgtttccg ctatgacaaa
    13681 atccagcttt attcaaattt tcttgacaca ggggacaagc ggatgagtat tttcataaac
    13741 aatgaactga ctgtaataga taggagaacg aatagtgtct ccaacaatcc aaaaacataa
    13801 taattcaaaa agacgataag actattggta agtaaaagtc cttgcggcat tggaagccga
    13861 aaaaatttgt ggaaaataat cggcggtatc gttataccgc catttgaggc cccgactgtg
    13921 tacaatgtgt aaaccccaag actgaacaaa agacttcctg aaatcagtga aaagaaatgt
    13981 gaggagatca tttccacttt tggaacaatc tctaaaacaa caggaaaaag cagacttccc
    14041 gctacagtct taaaaaatac tgattttcct aataggacag cggctagaag cagcatcata
    14101 agattgatgc tccaaacagt aactgagcgt tggatagaaa aggccgtttc aactaaatgt
    14161 ccgatggcgc ccactccggc ggaagctata tcgtgaggga gcaagaacca attcaatcca
    14221 actgtgataa agaaaagacc taaaaatata acgggtagtt tttttatttc ttgtgtcatt
    14281 gatatctcct ataaatgtta gccataaaag gctgtatatt tctttacttt accaaaaaaa
    14341 cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt atataaaatc gattgccagt
    14401 ttctgtttta ccaattaaaa tgatagtaat gttttgtaga aagttggaat ctgaccgatg
    14461 gataatcaaa gaaaaccagc attagatcaa ttaacaccgc aagaaaaaat ggcacgaggc
    14521 tctgcttgga tgaccgccag taatatcatt tcaagattgc taggcgctgt ctatatcatt
    14581 ccctggtatg cttggatggg cgaaaatgct aaggctgcca atggattatt caatatggga
    14641 tacaatatat atgcattgtt tctactgatc tctacagcag gaatacctgc tgcgattgca
    14701 aaacaaactg cacgctataa ttcattgaat gaatatggca cttcacgacg attatttatc
    14761 cgtgcattac agatgatggg cgggttaggc ctgttgtttg ctttgttcat gtacatcgcc
    14821 tctccttggc tagctcatgc ttctggtggc ggagaagaac tgattccaac gatgcgttct
    14881 ttgagtatcg ctgtattggt ttttccttgt atgagtgtga tacgagggta tttgcaaggc
    14941 aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag aacaggtggc acgtattttt
    15001 tatatgctat tggctacatt catcatcatg aaagtcatgg atggtgacta tgtgacggcg
    15061 gtcacgcaat caactttcgc ggcatttatc ggtatgctag caagtattct tgtccttttg
    15121 tattttttga agaaacatca agcgtataca tcggcgttta ttcactacag cgaaaacaaa
    15181 ataaatattg caacgaagga attgttgtta gataccgtta aagaagcgat tccgtttatt
    15241 attgtgggtt caggggtaac gatcttcaaa ttagtcgatc agttcacttt tatgaagatc
    15301 atgtctgcat ccaccgagta ttccaatgca cagttactgg atcttttttc gatttttagt
    15361 gccaatccag ataagttgac gatggtagtt attgcattgg caacatcgat cgcgtctact
    15421 gggttaccgt tgattacaga agctgttacg ttgaaagatc gagtaggtct ggcaaaattg
    15481 acaagcggaa atttacagtt gttttcattt tttatgtttc cggcgacatt tggtgttatg
    15541 ttacttgcct atccgctaaa tacattattt tatacaccgg attctctagg aagtaatgta
    15601 ctgatccaag caagttttgt aggactcttc ctaggactat atatgctagt ttccaacatg
    15661 ctgcaaggaa tgtttgaaaa caaggcggca attaattatt tggttattgg gtttctagtg
    15721 aaactccttc ttcaataccc agccattcgt ttatttgaag tatacggccc tttattggcg
    15781 acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa agagaatcca tgaagtagcc
    15841 cgctttaaac caagttttgt ctggcgcagg acattgctga tctttattct taccttgatt
    15901 atgcttttgg cttcgtggat cacaaagatg atttttggtg cttttttaaa cgaagatagc
    15961 aaattccagt ctctattgtt gattcttgta gtagcaggta taggggcttt agtgtatggt
    16021 tatttagctt tgaaaatccg cttggcagaa agactactag gaccaggaat agcgcgttta
    16081 cgacataaat taaaaataaa ataagtgaat cagaagagct agattgatgc tcttttgaaa
    16141 tgggggaaac gcatgcgttt agataagttt ttagcagata tgggacttgg cagccgcaaa
    16201 gaagtcaaag gattaatcaa aaaaggaacg atcgaagtca atggacaagt ggtgaaaagc
    16261 gataaagcgc aagtaaatga attcgaagat gcggttactt atttaggaga aatcattacg
    16321 tatcaaaaag atttttacta cattttgcat aaacctgcag gagtcatttc tgctactcag
    16381 gataatcatg atcaaaccgt aatcgattta ttggaagacc aggattatcg ggaagatctt
    16441 tttccagtcg gccgattaga taaagacaca gagggcctct tgattttaac gaatgacggg
    16501 gtgtttgccc atcagttact atcacctaaa aaacatgtgg agaaagaata tctagctgag
    16561 gttagcggta taatgacaga agaagatatc cgtttatttg cagaaggact gattattgat
    16621 ggcgatgagc agacattgcc ggcaaaactc caaattgatc aagtagatca agagacagaa
    16681 acttcaatta tccgtctgat tttgcatgaa ggaaagtttc accaagttaa gcgaatggtc
    16741 aaagcagtag gaaaagaagt cttatattta aaaagaatcc gtatgggaaa attttggctg
    16801 cctaaagatt tgaataaagg gcaatatcga cccatgacca aagaagagct agaacaagtg
    16861 agagaaaaat aattgaatgg atagctaaaa gaataagcag agtagttgta gcgccatctg
    16921 aaaagcagac ttgtccgtct gactttcgga tggctttttt gtgaaatttc ggcttatttc
    16981 cgtagaagct acttctggaa caccgtttgt tcggaataag ggactgtgac aagacttttg
    17041 ttacagtccc cattgttcct tgtgactcag agttagatag taaggacacg atctccctaa
    17101 tactcaggcc aaaacgctga attcacaacc tctgataaac ggtgttcaat cagctgaccc
    17161 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttcccgttt ttttctctaa
    17221 tactcaggcc aaaacgctga tttcacaacc tctcctatac tttgtttcat tttccctata
    17281 ctttgtttat ttattctcat tattgaaagc gctataatta cgttgcgaat gaatcgaaag
    17341 gggtttttag atgaaaaaac gaaaggggtt caatcatttt tttatgaatc tatggcgtta
    17401 taaagcactt gtcctgatgg ctgtaccggg tatgctttgg atgatttttt tcttttacat
    17461 acctgtaatg gcgaatgtcg tagcattcaa aaattttcat atttcagcag atgggttctt
    17521 ggctagcttg agggaaagtc cttgggtggg ttgggaaaat ttcaaatttt tattttcttc
    17581 ggatcaagca tttctcatca ctaaaaatac aattttatat aatgtagcat ttatcctttt
    17641 gaatctgctg atctcagtcg tatttgcaat cattatgagc gagttgagaa ataaacgact
    17701 cgttaaagtt tatcaaacga tgtccttact accttatttt ctatcttggg tcattatcag
    17761 ttactttgtt tacgcttttt taagtcctga caaaggaata ttcaatcagt ggatcactgg
    17821 acatggagga gaagctgtca attggtataa tgaaccgaaa tattggccgt ttatcttggt
    17881 gttcattggt acatggaaag ggattggtta taacagcatt atttactttg catctgttat
    17941 gggaatcgat ccgacttatt atgaagctgc tatggttgat ggagctagca aatggcagca
    18001 gatcaaacat gtgacgatac cacaacttat tccactaatg acgattttaa cgattttagc
    18061 cgtaggaaac attttccgag ctgattttgg cctgttttat aacattccta ggaattccgg
    18121 tgcattatat gatgtgactc aagttttgga tacgtatatc tacaatggcc tgacatccac
    18181 aggagatttt ggtatgacgg ctgctgcagg actttaccaa tcagttgtcg gctttgtctt
    18241 gttgatgatc acaaatacga tcgcccgacg ttttgatagc gattctgcat tattctaagg
    18301 aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag tccgctcttt caataagacg
    18361 gctaattttt tctctaatat aatgattgca ctctttgcta tttcctgtat tttgccattt
    18421 atatttgtta tcgccatctc ttttaccgat gagtccatat tgtcgactca cgggtacagt
    18481 ttttggccag aaaagttcag tatatttggt tatacgtact tgttcgacca gatgcaggat
    18541 aaaattttcc aagcgctttt cgtcactgta cttgttactg ttttaggtac actgatcaat
    18601 agcacggcta cttctttata tgcttatgcg atttcacggt caaactttcc attccgtcgt
    18661 ttttttaccg ttttttgttt gattacgatg ttgttctcac cagggatggt ggccaattat
    18721 cttgttatga caaatcttct tcagttaaaa gatacgattt gggcattgat cttgccaatg
    18781 gctgtcagtc ccttcaatat tattgtgatg cgcacattct ttaaacgttc ggtctctgat
    18841 tcgattattg aatcggcacg gatcgatgga gctagcgaac tgcgaatatt tgttcagatc
    18901 gttttaccgt tagctgttcc aggtattgcg acaatcagtt tatttgccgc attgggttac
    18961 tggaatgact ggttcaatgc tttactatac atccaagatg ataagctagt tccactacag
    19021 tatcttttga tgaaaatcca aagtaatatt caatatctga cacaaaattc aggagcagga
    19081 agccaattga caggcggatt agcctcagtt ccaggagaat ctgccagaat ggcgatcgta
    19141 gtcatctcga cgttgcctat tgcgatcagc tatccatttt tccagaagca ttttgtcaaa
    19201 ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa atataaatca taaaattagg
    19261 aggaagaaaa atgaagttat ggaagaaact agcctttaca ggtgtttcag cgttgttgtt
    19321 gggaacatta gcagcatgcg gaggttctgg ttctaaagac caggcagaag ctagcaattc
    19381 ggacactttg cagatgtatc aaatcggaga taaaccagat aatttcgatc aattgatgga
    19441 agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa gtcaatatca attacattgg
    19501 ctggggcgat tacgaaaaga aaatgaacgt catcatttct tccggggaaa attatgatat
    19561 cgcctttgcg aacaactatg tttccaatgc ccaaaaagga gcctttgctg atttgactga
    19621 attagcgcct aagtacgctg aaaaagctta taatgattta gatgaagcat atattaaagg
    19681 aaatctggtc aatggaaaat tatatgcttt cccagtcaat ggaaatgtct ttgctcaaca
    19741 agtacttact tttaacaaag ctttactaga taaatacgat ctttcaattg atggaatcca
    19801 atcttacgct gatgcagaaa aagtcttgaa ggaattccat gaaaaagaac caaacacggc
    19861 agcttttgcc attggacaag gcttcaaagt ccaagggaac tttgattatc ctttagggaa
    19921 tacgttacca tttgctattg atctggatgg agatgattcg aaaatcatca accaatacga
    19981 taatgaaacg tatatcggac tgttacgtac aatgcatagc tggtacaaac aaggactcat
    20041 tccaagtgac gctgcaacga gcaataagga ttatccacta gaaggaaata cttggctaat
    20101 gcgtggagaa acacaaggac cgtatgatta tggcgataca attttgacaa atgcagcaaa
    20161 acaagaactt gtctcaaaag caattactgt tccactaaaa tctacagctc aagcacaaat
    20221 ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa aaatcagtag aacttctagg
    20281 gctgcttaac agtgatccag aattattgaa tgggcttgtt tgggggatcg agggagaagc
    20341 atgggaaaaa gtcgatggtt cagatgataa aatcaaattg ctagatggtt atcagccaaa
    20401 tacacatatg tctgcttgga atacaggaaa caacaagatt ctatatacac aagagtctat
    20461 tacggatgac atgattgcca aacgtgatca atcgattgct gatgcagaaa cttcaccgat
    20521 tttaggattc agcttcaaca ctgacagtgt caaaacagaa ttaagtaata tttctaatgt
    20581 catgaatcaa tatttagatg gattgaatac agggactgtt gatccagatg aaacattacc
    20641 gaaattgaaa gacgctttgg acaaggctgg ctatgataag gtattgaaag aaatgcaaaa
    20701 acaatatgat gagttccgac aagaataata atgaataaag acaaaggggt tgtgacaaaa
    20761 gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa aagccagttt ctcggtatta
    20821 agctaaatat atgtaaaaat gtggaaagcc atattcacaa aatttttctt actactcgaa
    20881 actaagcggc tatttcacaa actcttggta aacggtgttc aatcagctaa ccctcggtat
    20941 tcgctcacaa aacgacaaaa tatgaggaac tatttttccg tttgtttctc taataatcag
    21001 gtcaaggtgc tgattgccca accttttttt gtataatgag aaagtataaa cttaatgttt
    21061 ggaggagaag atatgttagg gaaggttttg gaagagttat ttatccggat ctgggtcatt
    21121 ataaaactaa ccttgtattt ctggatatat acatttgccg gcgggatcat ctttggatta
    21181 ggtgcggcat ggaaaacagt caatgaacta ttttatttgt acggattcga gtataaagaa
    21241 atcacaataa aaagaggctg gaatatatat aaaagaaatt ttttaagagg caatctgcta
    21301 tttagtttat ttttatctgg tactgcttta ttgagttaca atttatattt atctgttcaa
    21361 atcaaagggc tcattttttt agctattgat tttattttac tttttgcctt gctctgccta
    21421 tttgctactt atcaatacag tatgatttta gatagtgaat atacgatctc tgttcctaat
    21481 ctgctaaagc taagttttat ttcggttttt tccagctttt cgtcttttct aaaaataatt
    21541 attggtagcg gtataatttt aggcgtgact tggcaattca aaggattgat cctgtttggt
    21601 gtcattggtc ttttgacggt ttggaatggt acgatgacaa cacattggcg tgaagaatta
    21661 gataaacagt tggaaagtta tgaataagtt tcggattcta ttttcaaaag gaaccttact
    21721 aaatcgtctg ttgcggaaat acgcggttat catgatcggt gtaacgatga ctgctaccgt
    21781 tatttttagt atccatactt gggaacaaaa tcaaaaacag gcagaaaaca tgacaagtga
    21841 tgccgttcaa agcacaagca gaatgctgaa tgataaaaca accttgagta ggatcatcaa
    21901 aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg acgacctact taacaaaacc
    21961 catagatcag tatctgatgt atgtatacga acagcaaaac agtaccgatg aattagtttc
    22021 tttcccaaat caaatcaaag atctctacgc aaattatgaa gaactaagtg cgatatatat
    22081 cgtgttgaat caactaccgg agtattatga atcttctaga gaaaacaaag gtggggaaat
    22141 aaaaaagggg acaccaaaat tactagacgc cttttatatc aagttgccta tcacacaagg
    22201 aggagctgag tcaggaagta tttttattgg ttttcaaaag aaagaactag atatgatttt
    22261 agaaaatcta acgtcattca atggactctc tttatacatg atttctggaa cgacaaatcg
    22321 actatacaca ttccatgata ataaaatagc taaagaaacg tttgaaagac aagaaatgat
    22381 catcacagag agtctgaaga aaaattcaca attgccgatt gacgatctgg aaaaaaacaa
    22441 ttttgttcaa caccaagaac tgtctgatga ttatcgtata ttagctgtac tagataaaaa
    22501 aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg atcattggta tcattatttt
    22561 agacaatata ctgcttatct ttctttataa aacattcaaa cgatattcac agcaagtatc
    22621 tatcgtcatg caggccatgg atcaaacagc agcagggaat ttagagacgc ggatcgacat
    22681 aacaaacacg gaatatgaac tgagggaact ttcaatcggt atcaacgaaa tgttggatag
    22741 catcaatcag tttgtagaag acatctacaa attagaaatc aaacagcaag acgcacatat
    22801 gcgggcgctt caagcacaga tcaatccaca ctttttatat aatacattag aatatatccg
    22861 aatgtatgca atcagcgaag gaagtgaaga attagcagat gttgtgtatg cgttttccgc
    22921 tttgcttcga aacaatacga atcaagagaa aacgatcact ttaaaagaag aactggactt
    22981 ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct aatcgagtag cctatcattt
    23041 tatgatcgat cctgatttgg aaaagatcga agtacctaaa tttgtgatcc agccattagt
    23101 tgaaaattat tttaaacacg gtattgattt tactcgattc gataatgcgc taagtgtcaa
    23161 agtcttgcag gaaggcaaac gggtaaggat catcatcaaa gataatggaa aagggatgac
    23221 tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat cctaaagtgg aacttcacgg
    23281 ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc agcttcggca gcagttatta
    23341 tatgtcgctt gagaataatg agacaggtgg actgaccgtt tctattacat tcaaggaggg
    23401 atgagcgtgt atcaagtgtt attggtagat gatgaataca tgattttgaa tgggttaaaa
    23461 aagataattg attggcaaag tttaggcttt caaatagtag caacagcaga gaatgccaaa
    23521 gaaggactag ctgtacttga acagcgacag attgatttag tagtaacgga tgtgacaatg
    23581 ccagaaataa atggactgga atttattgaa gcggctcaaa aagaacgtca taattttgaa
    23641 tttatgattt tatcaggata ccaagaattc gattatttga aaggaggtat gcaattaggc
    23701 gccgtgaatt atttgatgaa acccgtgaat aagttcgaat tggttgaaag cctcaagaaa
    23761 atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc aacaagaaat ctatcaagaa
    23821 ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa taagtgaaga agaaataata
    23881 ggacaaataa gtgagaagca gcataggata gtattgattc aactggcaag aactcatgat
    23941 actcttatca atagatggtt gcaagaacgg caacaaatct tttactatca acgaaattac
    24001 ggagaccgtg tattgatcac gttgttgttg gaggcagatg aagttgatgg cttctgttgt
    24061 tttgttcaag aaaactttcc tgatcaggaa tggctgatca gtatcgggga agagacagat
    24121 tctttggaca aaatccctga aacattccaa caagcgaaag acaatctaca gttgcatcaa
    24181 ttttatggag ataaagacga gcatgttttt tacgcagtac aagcagaaac aaaagatcaa
    24241 accatcgatt tttcaagttt cagacgcgtg ctgcaaaata aacaattgga agtcgctcaa
    24301 aaaatgatca ctgatttttt tgaacagttt cagttagcag ccatgatgcc ggaagatata
    24361 cgatattcca gttttttact gtttatggag attcaacggg aattgattga tctggaagat
    24421 gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa aaacggtgca agaacttcag
    24481 cagctacttc tgtcattcat caaaaagcat cagagacaga aaaagtacag tgacaatgtg
    24541 gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac cacttacact caaagaagtg
    24601 tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc tgttcaaaaa agaaacgaaa
    24661 aaaagttttt cagcttattt gaaccattta cgtatggaaa aagcgaaaca actgcttttg
    24721 cacagtaatc aaaacatcaa tgagatagca agtgagattg gctacaacaa caccacgtat
    24781 ttctctaaat tattcaagaa aatcgtcggc agatccccta aagagtaccg agaaaacagt
    24841 ggagaatcat aaaagaaaaa gccaatgtaa tgaaataact tataaaaaat tgtctagaaa
    24901 tctaattttg ggctgttctt attacttgag gcaaggcagc tttttcatga cctctcgata
    24961 aacggagttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag
    25021 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac gacctctcca
    25081 ctaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaatggca aaatatgagg
    25141 aactattttg ccatttgtct ctctaatact caggtcagaa cgctgatttc acaacctctc
    25201 aataaacggt gttcaaaagc ttgcatctgc ggtaataaga ccaaccaaac gaaaaatatg
    25261 aggatctatt tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    25321 cttgttaatt atagtttgat aaggaaaaag gcaaatcttt tgcttgatat ttcttagggt
    25381 gagggatgat ccctaattgg taagagatcg tgccgccttt cagcaaatct tgatgggtaa
    25441 aatataattt cgtatattct ctgtccatcc gtttgatttt atggataaat tgttgctgcg
    25501 gccgattagg cgttgcttca atcgtcaatt cctttccatt tgacaaaagc aaaacagcct
    25561 tgtcaattaa tggcataccg ataacatatt caccggttcc aggcgtgaca ggataaaatc
    25621 ccagactatt gaagatatac caagcggcca tacttccatt gtcctcatct ccagggtaac
    25681 ccattggaga atcgtcgaag gtctgagtca tcaactgttt aagcagtggc tgtgccattt
    25741 caggttgtcc tatataactg aacaagtaag gatagtgaaa acttggctgg tttgaaatag
    25801 ctaattgtcc aaattctatc gcggccatct cactcatttc gtggatctca aaaccatatc
    25861 ctcctgggtc aaacgttggg ggctgattgc acagctcaat gagtttttct ttatatctct
    25921 cactgccacc atattttccg atcagtcctg aaaaatcgtg ataaacagca aagctgcttt
    25981 gccaagcact tccttctgca taatcttgtc cccaacgatt agctgaaaat ggttctctga
    26041 acttgccatc tttatctttt gaacgcataa atcctgactc atagtcaaag atattttgat
    26101 agttcaatga ttgctgctga tatttcttcg tcatttcctc ttcttctagt accttggcaa
    26161 cttggctaat acaaaaatcg ctataagcat agtctaatgt gtgattgact gattcgtggt
    26221 agtgattagg aacatagcca tatgtcaagt aatcttctgt tccttggcgt ccataatttg
    26281 gatgtttgct ttgaaccgta gcaccttttt ccattgccgt tagaaattca ggcatcaagt
    26341 ctcgtcggat atcttttaca gcagcatctg caatgaccgc atcgatcaat gtcccaggca
    26401 taagtccacg ttcatcagga gacagccatt ttggcaaata gccactttct ctatagctgt
    26461 tcaaaaaacc ttccagcatt tcttcgtatt tttctacagc aatcaaagaa taaagaggat
    26521 aaaccgtttt atacgtatcc caaaacccat tatttgtata gagaacacca ggttttatag
    26581 atcgactagt cgtatcatag tggatttttt gcccactagg atcaatttca taaaatgttt
    26641 gtggaaataa aaacacccga tataaattat gataaaacgt ccgcaattgt tgttgatcat
    26701 ggtgtgtgag ctgtatacga tcaaaatagg actgccattg ttcttgactg tgagcaaggt
    26761 aagtctcttt gctttggttt tgttctagtt ccaggttata agctgcttgt tccgagctga
    26821 taaaagatgt cccaaatcgg atagtttgtt tttttgtctg ttcaaaatac aacgggatcg
    26881 agtcgctatt tttgttggaa acagctaact catcaattgt taaaggatat tcaaatctca
    26941 atataaaata aaaagaaaaa tcaggatctt ctgaaccagc aaaatgtgtg atttttcctt
    27001 ccactgtgta aggatctata acaaagagct gatggcgtcc cggaaaggat aaaagtaaac
    27061 cgctgtcatt tttgctgtaa tcaatagata agattccgcc atacatgcta ggtatcaatg
    27121 tagaacagat gccataacgt aattgacaga tcgacagatg agtcgggcaa aatatacttt
    27181 cttctgggcg gtaagaactt tgagcatgga aaagggtgtt ctcctgtagt tttccattga
    27241 tcggcgtaaa cagcatatga ctgaaatcac ccatccaagg acttggttga tgggtaagac
    27301 ggtagccttg aaaaacgcgg tcatcaggat gaaaccacca gctgccgttt tgatccgtag
    27361 tttgcggtgc aaaataattc atcccaaaag gaacgccagt ataaggctgg cagttcccat
    27421 tagagaaatt tggctgattc gctgttgcat gtctagtatc tatttgaccg atattcattt
    27481 tatttctcct ttgttgattg tcttttgatg aatgaaactg gaaatttact atcggatggt
    27541 cgatcttttg tctctaacca agtgaccaaa gcatctcccg caagcttacc taactgttca
    27601 aaatcttgtg cgatggttgt cagtggcggg tcaacaagag ctgctgcttg gatatcatca
    27661 aatccgataa ttgaaaaatc ttcagggata tgataaccat gctgtctcaa gagattcatg
    27721 gtctggatag cgacaacatc gttttcacaa acaaaagcag ttacttcata cttagtgata
    27781 taagaaagca attcatctaa tgtcgcgctt ttgtcatcca gtgaagtgtg gaaagtcaaa
    27841 ccagctttgt ccaatgcttc aaggtaacca aggtaacgct gtcgaacgga ttgtggatga
    27901 gtctgcgtac caaacaaata agcgattttt gaatgtccct gttcgatcaa ataagaggca
    27961 gcaagcgcac cgccttggaa attatcggaa agaacggtag gaaaaggtaa ctcatagatt
    28021 tttttatcta atatgacgac agggaattct ttcaatgaaa gctcaaaaag taagtcaaga
    28081 tgttgttctg tatcaagagc ataataaatc aatccgtcaa attcattgat tagctctttt
    28141 gctgttttat tttgtagaaa gtcaagcgtc gtcatcatga catcaatctg tttatcttgc
    28201 atcacagggt tcaatccctt gctaaaatca ccaacagaca aatcagcgac atatggaaga
    28261 agaaacagaa tccgtcgtgt ttttgtagga gaagcaagaa tctgttccgg agaagcggat
    28321 ttaacgaaac tgcctttccc gcgaacgcga taaataagtc ctaattgctc taattcagtc
    28381 aatgcacgtt tggaagtgat tctgcttact ttataggtac gtgaaagttc tttttcagtt
    28441 ggtagttggc tgtctacagg taattcacca gttaaaatag ccgtttgcaa atcattaaat
    28501 attgtctggt acaatggttg agtcatataa acacctctta atgatatatc atattcaaat
    28561 aataccataa tggatgaaaa agtaaataaa aagatatttt ttgaaagcgt agacataatt
    28621 ggctgtatga agtataatct ttaaagatat atcaaataga aagaaggttt tattagtggt
    28681 ttataaagat ataccaaaat cggtgcagaa atttatggaa gatatcacac aaaaatgtgg
    28741 ggatatccat gcggactggg caaagaattt tacagcagca tttgccaata ctctgttgac
    28801 aacagtcaaa aagcaaaatg acggaaccac ctttttattg acgggagaca ttccagcgat
    28861 gtggttaaga gactctactg cacaagttcg tccttattta gtgattgcaa aagaagatga
    28921 ggaattagct gacatgatca gcgggttagt caaaaagcaa tttttttata tcaatatcga
    28981 tccatatgcg aatgccttca acgaagaagc taacggtgct ggccaccaaa cagatcatac
    29041 aaaaatgaac gattggatct gggaaagaaa atatgaaatt gattctttat gctacccaat
    29101 acagttggcc tatcttcttt acaaaaatac aggaaaaact gatcaattca atcaatcttt
    29161 tattgaaggt atcaaaaaaa tcttggacgt gttcaaaaca gagcaagatc atgaacactc
    29221 tccttataca tttgtacgag acacgacaag aaaagaagat acgctgatca atgacgggcg
    29281 aggtgcaaaa acagctttta caggaatgac atggtctggt ttccgtccta gcgatgatgc
    29341 ttgccgttat ggatatctgg taccatccaa tatgtttgcg gtggtcgtgt taggttatgt
    29401 ggaagaaatt tttcaaacga tactcgatga taaagaaatt gtacagcaaa cacagaaatt
    29461 gaagaaagag atccaagagg ggattcagaa tcacggacga acggttaatc aaaaagggca
    29521 aacgatttat gcatatgaag tagatggaat gggaaacgcc acagtcatgg atgacagtaa
    29581 tgtgcccaat ctgatcgccg caccttactt gggatattgt tctgaacaag aggaagatta
    29641 tctgcaaaca agaagaacat tgttgagtaa agaaaatcca tatttctatg aaggaaagta
    29701 cgcaaaagga attggatcct cccatacgcc agaaaaatac gtttggccga ttgcattagc
    29761 tatggaagga atgacaacgt cagacaaatc tgaaaaagag cggatattga atttacttgt
    29821 agcaactgac gcaggaacac accttatgca cgaaggattc gatgtgaaca atcctgaaaa
    29881 ttatacgaga gaatggtttt cttgggcaaa tatgatgttt tgcgaattag tcatggatta
    29941 ctttgatatt agagtagaaa agtaggaggt atcgtaaatg aaaaagaaag tatatatcat
    30001 ttctcatagc cactgggatc gtgaatggta catggcttac gaacagcatc acatgcgact
    30061 gattgaattg atagacgatc tactagagct atttgaagtt gatccgtcct tcaacagttt
    30121 tcatttggat gggcaaacga ttattttaga tgactatctt caagtaaggc cagaaaaaag
    30181 acaggcaatc caacaagcaa tcaatgaagg aaaattgaga attggaccat tttatatctt
    30241 gcaagatgat tttcttatca gctcggaatc gaacgtgaga aatatgctga ttggcatgga
    30301 agaaagccgt aaatggggaa cccctgtcat gttaggctat tttcccgata catttggaaa
    30361 tatgggacag acgcctcaat taatgaaaca agctggtatt tcagcagcag cgtttggccg
    30421 tggtgtaaaa ccaattggct ttgataatca agttttggaa gcagaaaact attcttccca
    30481 atactcagag atgtggtgga aaggccctga tcaaacagca atctttggtc tgctatttgc
    30541 caattggtat agcaacggga atgagattcc agtagaaaaa gaagcagctc ttgccttttg
    30601 gaagcaaaaa ttagcggatg cagaacaata tgcctctacg aaccacttat tgatgatgaa
    30661 tggtgttgat catcaaccgg tacaaaaaga tatatcaaaa gcaatccatc tcgcaaatga
    30721 actgtttcca gattacgagt ttattcattc taattttact gactatctgg aagcagttca
    30781 aaaagatgtg ccagaagact taggctcagt agaaggagaa ttgacaagtc aagaaacgga
    30841 tggctggtat actttagcca atacagcatc tgctcgggtt tacttaaaac aatggaatac
    30901 caaggtccag cgacagctag aaaatattac agaaccactt gcgacgatgg cttatgaagt
    30961 tagtggaaac tatccccatg atcaattaga ttatgcttgg aagacattaa tgcaaaatca
    31021 tcctcacgac agtatttgcg gttgttcagt cgattctgtc catcgggaaa tgatacctcg
    31081 ttttgaaaaa gcagatgaag ttggaaagta tcttgcacaa gattccttag aacagctgac
    31141 cgcagcaatc gatacgaccg gattcccaaa agacagcttt ccatttgtca ttgtaaatac
    31201 tgcaggaatg gataaaactg gtgaagcaga aataaccatc gaattagaaa gaaaaagatt
    31261 cgctgaagga atacctgaac aactatatca agagttagaa aatttgccta aacgtaagta
    31321 tcatgtagaa acaaaaagtg gggccactat accagctatt ttatctgaag aaactgtcca
    31381 atttggttat gatttgccga aagaccgttt ccgtgtgcca tatatggcaa gaatgatcaa
    31441 agtgacttta ccattagaaa acatgccagc attttcttgg gaaacattcg cgcttgtaga
    31501 aggggaagcg aaagcagaag aaaaggaaac aatgattcat cagagtggac gaatcatcga
    31561 aaacggccct ctacatttga cgattgaaaa aaatggtacc attacaatgg aagatcgcaa
    31621 aaacaaacga aagttgaata atctgcatat ttttgaagat atcggcgata taggaaatga
    31681 gtatatcttt aaacaacctt tctgtgatca gccaatccta tccagtaaca aagaaaactc
    31741 tgaagtaaaa gtattagtag atacaccgga aatagcaaaa ataagtattc tacaggaaat
    31801 ggaaatccct gtttctgcag acgagcgttt agaaaaagaa cagcagatgg ttgtggaatt
    31861 tcgttatcga aaagcagaac gatccaaaga aaaacgaatc cttcaaataa aaacaatcat
    31921 gacaatcaga aaagatagta aaaaaatcga ctttgaaacg actctcgaca atcagatgaa
    31981 agatcatcgc ttacgtgtct tgtttcctac aaagttacat gtggaacatc acgaagcaga
    32041 tagtattttt gaagtggtga agcgcccaaa ccatgtgtct aaaagttggg aaaaccccac
    32101 gaatccgcaa catcagcaag catttgtcaa tattcatgat gaagagtacg gtgtaacagt
    32161 cggaaacttt ggtttgaatg aatatgaagt aacagaagac ggacaaatcg cagtgacgct
    32221 acttcgcagt gtgggagaac taggcgattg gggatatttt ccaacaccag aagcacagtg
    32281 tctaggtgag catcggttca attacagcat tgaactacat ggaccagaag aaaaattctc
    32341 tacttatttg catgcatacg cagcacagat tcctttttcc actcaacaaa tcaaacatca
    32401 tgaaggaaca ttgatttcaa aacaacaata tttaacgata aaaagtgaaa cgtttgcaat
    32461 tacagctttg aaacgcagca agtttagtga taaagtagta gtaagaggtt tcaatatgtc
    32521 tagtcattta gaaaaattgg aaataactaa agacaatgga aaaactgtca tattgaatct
    32581 gcttgaagaa ccaacgaagc aagcggttgt cccaataatc caaccttatg agattcggac
    32641 aatcggattc gaggaggaaa acgaatgtat ggcggaatag aagcaggcgg aacaaaattt
    32701 gtgtgtgcgg tttcagatgg tggagaaatt ttagaaaaat taagtatccc taccacaaca
    32761 ccagaagaaa cattggatca ggtatttgaa ttttttgatc gttatgaatt gaagggaatc
    32821 gggattggct cattcggccc gattggtatc gacaaaacaa aagataatta cggatacgtt
    32881 ttggcaactc ccaaaaaagg gtgggcacaa ttcgattttc ttggcacaat caaggaacgc
    32941 tacaatattc ctgctgcttg gacaacggat gtcaatgctg ctgcatatgg ggaattgaag
    33001 caaggagcag cacaaggaaa aaatagttgt atttacctta ccgtgggaac aggaatcggt
    33061 gcaggtgtcg tgatcaatga agaaattttt tcagggatcg cacatcctga aatgggacat
    33121 atttgggtga aacgccatcc agatgaccat tatgaaggta cttgtcctta tcataaagat
    33181 tgtttagaag gattagcagc aggaccgtct attgaggcac gcacagggat caaagggcaa
    33241 gatctgccgc aagatcatcc tgtttgggct attcaggctt attatattgc tcaagcactg
    33301 atcaactata cgttgacgtt agcacctgaa aagattattt taggcggtgg ggtaatgaat
    33361 caggatcatc tccttcaaaa aatccgtcag caatttgttg aattgatggg tggttatatg
    33421 gaaacacctc aagtaagtga atatatcgtt cgttggggac tgccgaatga aagtgggatt
    33481 atcggtagtt tgctgttagc agaacacgaa catcaaaagt aagcagacaa attacacaaa
    33541 aagaacagag tcttatcatt atctgctgat aagactctgt tcttttttat ttctattccc
    33601 ataatccagg taaagcttta ccgactattt tttcttgagt aaatggatgg atcatgtgca
    33661 gttctaatgc atggagcatc agtcgttcag ctgttctatt ttggtagaga gggtctccaa
    33721 cgatcggatg tcccttatga gctaaatgaa ctctgatctg gtgtgtacgt ccggtttcca
    33781 aaacacaata gacttgtgtt tgttcagaaa gtactttgtt gacagtgaca tgtgtcaagg
    33841 cgctctgacc atttttatca tctacgaccc tttttctccg atcatggcga tctcgtccaa
    33901 ttttattggt gatggtcata tctttggcaa tttctcccca tacgatggct tgatatctcc
    33961 gatagatcat tttttgttcc aacattcttc caagtatcgg caagacaaat ggattttttg
    34021 caaataaaac ggcaccgctt gtttctttgt ccaagcggtg gacaacgtaa ggtcgttgct
    34081 gtttagtgtc gagataagca gctaaatgat tgagtaaggt actgttttct gtcggttcat
    34141 ttggatgagt cttcattcct gcaggcttat ccactataat caaatgctca tcttcatata
    34201 aaacacggat gtgcttcgca tctcctaatt gaacttcttg ataattataa tctgtttctt
    34261 cgaataacaa tgtgatctgg tcattgccct ttacttcatg atggaacaaa gcaggttgtt
    34321 gattgatcca aacgttttta cgtatacgca aaaagtgtcg aacctttcta ggaactaacc
    34381 attccttttc aagcaagtcg cgaattgttg tttcaggatg gttcataggt aaagtgatcg
    34441 tgattttcac aagtgtactc ctttcagtgc aattgcagac taattgtacc atttttaaaa
    34501 catatttaag aaaaaactta catttttctg ttcattctta tgttactcta cttgcactaa
    34561 cgtaaaaaaa catattgaac tacaacttaa gcaggaagaa aacactcttt cactatggta
    34621 aaataagacg attgaatcaa atagacagga gaagaagcaa atggattttc aaagcatttt
    34681 agaaaagata aaagctgctt tgatcagttt ttggcgttgg atcagaccat atctcggaca
    34741 attccatcga tggcgaaagc gcatctggaa aaaatatcat gtgaacaaat tgattttgct
    34801 gattggtttg atctgtgtct tagtgacaag tatctacctc tttattttag caaaacaagc
    34861 aaatgtcgaa acgttaaaat caggattaag ccaatcgaca gtcatttatg ataagaaaaa
    34921 cgaagaagct ggcacactgt acgcccaaaa aggaacatat gtagaattaa acgcgatttc
    34981 tccctatatc caagatgcag taatctcgac agaagatagg aatttttacg agcatcacgg
    35041 gtttgatatc aaagggattg cccgtgcagc agtccgaatg gtcttgtctg gcggaacatc
    35101 agggggcgga ggaagtacga tcactcagca gcttgccaaa aatgcgtatc tgacattaga
    35161 ccaaacgttt gatcgaaaag cgaaggaatt atttttggca atcgagatcg aaaagaagta
    35221 cagcaaagaa gaaatcttga ccatgtatct taacaatgcc tattttggca acggggtatg
    35281 gggtgtccaa gacgcttcca gaaagtattt tggagtagat gcaacgaatg ttacgctgtc
    35341 tgaggctgct actttggcag gaatgctgaa aggtcctggt atttataatc cgatcgatca
    35401 cccaaaaaat gcgaatgatc ggaaaaacac agtactaagt gtaatggaag aaaacggaaa
    35461 aatcacaaaa gaacaagaac aagcagaaag ccagacagat atcagcgcgt atcttaatga
    35521 cacatacgat aattcagatt ctggttatcg ttatccttat tattttgatg cagtgatcga
    35581 tgaagcagtt aacgagtatg gtcttgatga agaagatgtc atgaataaag gctataaaat
    35641 ctatacagct ttagatcagg attatcaaaa gcaattagaa gctacttatc aaaacagtgc
    35701 attattccca gcaaatgctt cagatggtgc catggtccaa tctgcttcag tagctttaga
    35761 tccgaataca ggtggtgtac aagcactagt aggacgaaga ggagatcatg tctttagagg
    35821 attcagcttt gctacacaga tgcgtcgttc accaggatca acgatcaaac cattaagcgt
    35881 atatacacca gcactggaag ctggatacaa gccttccagt atcttgaaag acgaaccgca
    35941 aagttattac gatgcgcata attttgacgg gacataccaa ggagaagtac cgatgtatca
    36001 agctgttgca caaagtttga atttgcctgc tgtatggctt ttgcatgaga tcggtttaca
    36061 aaaaggatat gataaagcag aagaattcgg cctgccttta gcaaaatctg ataagtatta
    36121 tggcttagcc cttggtggac tggaaaaagg agtatctcca ttgatcatgg cgggtgctta
    36181 cagtgcattt gccaatgatg gtcagatgta tacgccgcat cttatcacga aaatcgttga
    36241 ttcgactgga gcggtgatcg tcgataacac aaacaaaaaa ccaaaacagg tcatatcaaa
    36301 ggaaacagcc gatgaaatga ccagcatgct tcttggtaca ttttcaaatg gaacagcggc
    36361 tgctgcgaat cctgcaggtt atacgatagc agggaaaaca ggaaccacag aaactaattt
    36421 cgatgccaca aaagcaaatg accaatggat gatcggttac acgccagacg tagtgatttc
    36481 gacatggatg ggttacgaag aatccagcaa gctgcactat ttggaaggga caagtggaaa
    36541 tgttgtcggg aaagtcttca aatcagcggc agaaggcatc cttccgtata cttcaaaaac
    36601 aggatttagt gtagcagatg cctatgccac aggaggacaa gtggtagctg cagatcaagt
    36661 tggaaacaca ggaaattcta atggagagtc tggaaattgg caagataact tgaatcaata
    36721 tggtgaacaa gcaaaagaag ggctgaaaaa cttcggcgat atggtgaaag aaggcgttca
    36781 gggaattggt gaagcggcta aagacctctg gcggaaatat caaggagagt agcccctttg
    36841 aaaacgaata accccatgtt acaatagaga gtaactacta aaagaaaaga gggcataaca
    36901 tgtcaaacat ctacgatagc gcgaatcaaa tcgaacgtga gatccgtgaa ttaccagaat
    36961 tcaaagcgtt gaaagaagcg tacgagaaag tacaagcaaa cgaagaagca cacaaactgt
    37021 tcaaagactt ccaagcgatg caaatcgaat tacaagaaaa acaaatgagt ggacaagagt
    37081 tcagcgacga agatgcaatg aaagcacaag aaatggctgt gaaaatccaa tcagaagaag
    37141 taatcaatga cttgatgcaa aaagagcaag gcttcagtac aatcatcaat gacttgaacc
    37201 gtatcatcat gacaccagtt cgcgaccttt acagcgaata aaactatttt atgaaacaac
    37261 agagggtgtg aaagagacgt tcggccttga gcgatgacaa aattgcttct tattgccgat
    37321 aggctgtctc ttgaacacca tttaccaaga ggtcgtgagc ctaccgttta actctgagtt
    37381 acaaggaaca atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg
    37441 aggagttggc aggagaacac cgtttattca gaaataggag ctacgacaaa agtcttgtca
    37501 tagctcctat tttttgtcat tcatttttca agcggtagaa atagcctttt tgagctaaca
    37561 gtttttcgta agtacctatt tcaacgattt ttccttcatc gatcacaacg attttatctg
    37621 cctctttgat cgttgaaagc cgatgagcca caataaaagt agtgggtgtt tctgtcaggt
    37681 aattcagggc ttgctggatt tttttctctg actggttatc taaagctgag gttgcttcat
    37741 ctagcaaaat gatttttggc tgacgtatca acgctcgtgc aatggagata cattgctttt
    37801 ggccaccaga cagcttgttg ccatgttcgc ctactaaggt atccagacca tcaggaagct
    37861 ggtcaatcag tgaagaaagt tgcgcagctt cgatgacttc atctaaacgt tcttttgata
    37921 cgtttttcaa gccataagtg atgttctctt tgatcgaagc ggagaacaac aaagtggttt
    37981 gaggaacaac ggctaaataa tttcgcacac ttcgcatatc cagttcatcc aaaggctgat
    38041 catctagaaa aatttttcct gaagtcggct ggataaatcc aatcaataag ttaaggagtg
    38101 tagattttcc agagccagat ggcccaacaa aagcaattgt ttctccagct ttcacctcta
    38161 gagaaaaatg atcgataatc ggtttgagtg agtccggata aagaaaagaa acatcctcaa
    38221 aacgaaaatt tccatccaaa tgttggattt ttttcttacc gtgataagtt tctacatcgc
    38281 cagcaatcaa cagttcgcca atagaagtga tagattccat gccttttgaa atcgtaggaa
    38341 tcagcgtgat cattccagaa acggaattga cgatcgtcgt gaaatacgtc tgatacatca
    38401 cgacatcgcc gggttggatg cgcccttgta aagctaatat cccagtgaaa atcaaacaaa
    38461 tcagctgaaa tatctgaaaa ctagcccagc tgactgatcc gaaaacagat tgcaggatat
    38521 ctaagtggaa tcctttttca tagatttgag taaaatgctt atttaagcga gttgtttcgt
    38581 gtttttctaa cccatgagct cgagcaatcg gtgctaattc gatcacttcg atcacttttg
    38641 cagaagtctc ttccatttct tgcctgaagt ctgagttgga ctgtctgatc cttttacgga
    38701 aaaagagagt aacgaatgcg gcgagaggaa cagtcaataa gaaaaacatg aaaacaacag
    38761 gacttttata aagcgtgata cctaaagcta cgaataaatt gacagcgata tttaatccgc
    38821 tgacaaatac ttgagaggac aacgtttgga tagcttctac atcacggatg atctttgact
    38881 ggattcgtcc agattgcaat tctttttgat aaggaataga tagttcttgg attttcttga
    38941 taagggtcgc tcttagtcca gcttctactt gacggatcga agtgctatgg tattttacat
    39001 gaaaataatt cgtcaatata ttttgaccaa tcagacaagc catcaaaaaa gcgttgaaat
    39061 aaatggcctg caactgagaa gaatctttcg atgaagctgc attgattaca ttggcaatag
    39121 cgatcggcat taaccaagat ggaagatgtt tgataataaa aaagagtcct gacatggcaa
    39181 aacgccaata atttccctga taaagatgca gcaatgtctg aaaagcattt gcatgatatt
    39241 tttgataact tttctgtatt cgttcctcca taatcttctc cttaacaatc ttggtaaata
    39301 aactgcaagt gttctattgc ttcaggaaca taaatctcag gagtagcttc aagagtggcg
    39361 tacattagag gacgctcata tttgatataa tgcagtaccg ggcggaaatc taaacagccc
    39421 ttgccaacag gtaccatgcg gatctttcca ttttccatga tatagtcttt gagatgaagc
    39481 gctgtaatat aaccatgtaa attgtccaag gcacctcgga tcactttttc ctgatcagca
    39541 tgattttctt tatgcatcaa attcgcacaa tctaaaatga tttttaaatt tggtgaagct
    39601 acttcatcga ttaaacgttt agctaactga tagttataga ttggatgatt gatccctgct
    39661 tcaacagcaa cagttacgcc aaaattttct gcatgagcga ctaattcaat gatagaattt
    39721 ctggcgatct gataagcttc ttcagtaaaa ttttcttttg tgtaaccgac agagacacta
    39781 ccggtttccg tagcaatcat cttggcttga taggcagagc acagagcaat ttgctgtttg
    39841 aaagttgcca atgcttgtgc acgaacttct tgatttttgc tggataggtt gatatagcat
    39901 cctaaaatgg aaagttcgat attgtttttt tggaagtatt caccaaaata ggcagcactc
    39961 ccaagtgtca tgtcagttaa tttaagttgt tcaggaaatg ctttttgtgg tgcaaattgg
    40021 atatgggaca agtgaagctg tgatactttt tgaacgattt cttcacgatt tcgaacatct
    40081 aaatcgtggg cacggatgcc taaatttaat ggaaacatct caaactcctt tttgtaaata
    40141 attagcaaga ggcagacggg attcttttat caattcaaca attattcggg caactttatc
    40201 agcgccttct ggactaaagt gggtattgtc ttttagtcct tctgggtaat ttttacaagt
    40261 atcaggcata agatgcaggt agaatttttt tgtctcttct tttgagaagg tctggaacaa
    40321 ttcttgtgat cttgagaaca catctaaaag aggaatatgg tgtttctcag caaatgcacg
    40381 catcgctttt ggatagtctc caagtatatc tggattcaat gttccgtttt ctaagtaatc
    40441 tcgtcgcgtc acagatgtca gtaagaccat ttggacgttc gctttttttg cgcattcaaa
    40501 aaatatctgc agatttttta aatagctacc gaagggaagc gtacctcttt cttcttggat
    40561 tttctggtcg ttatgtccaa attgtacaaa taagaaatca ttcggctgaa actgtgaagc
    40621 tagacgggct aaccttcctt cctggataaa tgatttggtg ctgcgtccat tctttgcttg
    40681 gttattgatc tggatctcgg gtgaaaaata agcagagatt ttttctcccc aacctgtctc
    40741 gggacgcttg ttttcatctt tttctgccgc agtagaatca ccggcaatat ggatagtagg
    40801 catgttatct cttctttcat aaaggtttat aggcaatgaa gcagaacagc aagagaagaa
    40861 gacggagcaa
//
LOCUS       DACDCV010000014        39613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDCV010000014 DACDCV010000000
VERSION     DACDCV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.014"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..368)
                     /locus_tag="IV041_000756"
     CDS             complement(<1..368)
                     /locus_tag="IV041_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381571.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFL"
     gene            complement(665..3046)
                     /locus_tag="IV041_000757"
     CDS             complement(665..3046)
                     /locus_tag="IV041_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8381572.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3036..3683)
                     /gene="recU"
                     /locus_tag="IV041_000758"
     CDS             complement(3036..3683)
                     /gene="recU"
                     /locus_tag="IV041_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8381573.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3747..4286
                     /locus_tag="IV041_000759"
     CDS             3747..4286
                     /locus_tag="IV041_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8381574.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4376..4795
                     /gene="gpsB"
                     /locus_tag="IV041_000760"
     CDS             4376..4795
                     /gene="gpsB"
                     /locus_tag="IV041_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8381575.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4817..5184
                     /gene="rnpB"
                     /locus_tag="IV041_000761"
     ncRNA           4817..5184
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV041_000761"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5393..6559
                     /locus_tag="IV041_000762"
     CDS             5393..6559
                     /locus_tag="IV041_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8381576.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6605..8101
                     /locus_tag="IV041_000763"
     CDS             6605..8101
                     /locus_tag="IV041_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8381577.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8182..8865
                     /locus_tag="IV041_000764"
     CDS             8182..8865
                     /locus_tag="IV041_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8381578.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9510..10700
                     /locus_tag="IV041_000765"
     CDS             9510..10700
                     /locus_tag="IV041_000765"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8381579.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10802..11755
                     /locus_tag="IV041_000766"
     CDS             10802..11755
                     /locus_tag="IV041_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8381580.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11824..13173
                     /locus_tag="IV041_000767"
     CDS             11824..13173
                     /locus_tag="IV041_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8381581.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13356..13679)
                     /locus_tag="IV041_000768"
     CDS             complement(13356..13679)
                     /locus_tag="IV041_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381582.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13808..14971
                     /locus_tag="IV041_000769"
     CDS             13808..14971
                     /locus_tag="IV041_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381583.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15041..16390
                     /locus_tag="IV041_000770"
     CDS             15041..16390
                     /locus_tag="IV041_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8381584.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16435..16866)
                     /locus_tag="IV041_000771"
     CDS             complement(16435..16866)
                     /locus_tag="IV041_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381585.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17254..19896
                     /gene="alaS"
                     /locus_tag="IV041_000772"
     CDS             17254..19896
                     /gene="alaS"
                     /locus_tag="IV041_000772"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8381586.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20096..20692
                     /locus_tag="IV041_000773"
     CDS             20096..20692
                     /locus_tag="IV041_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8381587.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20814..21962
                     /locus_tag="IV041_000774"
     CDS             20814..21962
                     /locus_tag="IV041_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8381588.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(22000..22413)
                     /locus_tag="IV041_000775"
     CDS             complement(22000..22413)
                     /locus_tag="IV041_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8381589.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22492..22923
                     /locus_tag="IV041_000776"
     CDS             22492..22923
                     /locus_tag="IV041_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8381590.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23022..23255)
                     /locus_tag="IV041_000777"
     CDS             complement(23022..23255)
                     /locus_tag="IV041_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8381591.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23438..25108
                     /locus_tag="IV041_000778"
     CDS             23438..25108
                     /locus_tag="IV041_000778"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8381592.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25255..25641
                     /locus_tag="IV041_000779"
     CDS             25255..25641
                     /locus_tag="IV041_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381593.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25628..26326
                     /locus_tag="IV041_000780"
     CDS             25628..26326
                     /locus_tag="IV041_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8381594.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26331..26795
                     /locus_tag="IV041_000781"
     CDS             26331..26795
                     /locus_tag="IV041_000781"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8381595.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26792..27703
                     /locus_tag="IV041_000782"
     CDS             26792..27703
                     /locus_tag="IV041_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8381596.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27938..28477
                     /gene="pyrR"
                     /locus_tag="IV041_000783"
     CDS             27938..28477
                     /gene="pyrR"
                     /locus_tag="IV041_000783"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8381597.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28491..29774
                     /locus_tag="IV041_000784"
     CDS             28491..29774
                     /locus_tag="IV041_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8381598.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29787..30713
                     /locus_tag="IV041_000785"
     CDS             29787..30713
                     /locus_tag="IV041_000785"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8381599.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30732..32012
                     /locus_tag="IV041_000786"
     CDS             30732..32012
                     /locus_tag="IV041_000786"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8381600.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            32017..33093
                     /gene="carA"
                     /locus_tag="IV041_000787"
     CDS             32017..33093
                     /gene="carA"
                     /locus_tag="IV041_000787"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8381601.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33098..36283
                     /gene="carB"
                     /locus_tag="IV041_000788"
     CDS             33098..36283
                     /gene="carB"
                     /locus_tag="IV041_000788"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8381602.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36337..37113
                     /locus_tag="IV041_000789"
     CDS             36337..37113
                     /locus_tag="IV041_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8381603.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37110..38042
                     /locus_tag="IV041_000790"
     CDS             37110..38042
                     /locus_tag="IV041_000790"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8381604.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38039..38755
                     /gene="pyrF"
                     /locus_tag="IV041_000791"
     CDS             38039..38755
                     /gene="pyrF"
                     /locus_tag="IV041_000791"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8381605.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38762..39451
                     /locus_tag="IV041_000792"
     CDS             38762..39451
                     /locus_tag="IV041_000792"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8381606.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39424..>39613
                     /locus_tag="IV041_000793"
     CDS             39424..>39613
                     /locus_tag="IV041_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381607.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRF"
BASE COUNT        12796 a         6975 c         8593 g        11249 t
ORIGIN      
        1 aataaaaaaa taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc
       61 atcagaaaaa tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga
      121 gagcttcttt ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata
      181 aatgcaagga taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct
      241 tttttattct tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt
      301 atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc
      361 aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact
      421 aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta
      481 tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca
      541 tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat
      601 ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt
      661 tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat
      721 ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg
      781 agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg
      841 ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc
      901 tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa
      961 tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt
     1021 caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg
     1081 aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga
     1141 caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc
     1201 ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat
     1261 ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg
     1321 taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg
     1381 gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga
     1441 ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg
     1501 ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag
     1561 agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag
     1621 ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt
     1681 aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag
     1741 tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca
     1801 aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg
     1861 aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa
     1921 tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt
     1981 cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt
     2041 ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt
     2101 cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg
     2161 attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg
     2221 tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg
     2281 cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca
     2341 atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg
     2401 tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct
     2461 cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt
     2521 tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca
     2581 aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc
     2641 gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat
     2701 cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat
     2761 cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt
     2821 tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa
     2881 agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat
     2941 gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag
     3001 taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct
     3061 tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa
     3121 ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt
     3181 cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag
     3241 cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct
     3301 ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg
     3361 gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt
     3421 gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact
     3481 ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa
     3541 tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg
     3601 atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg
     3661 ccatttggat agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa
     3721 gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt
     3781 atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa
     3841 atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca
     3901 gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat
     3961 atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg
     4021 aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta
     4081 gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc
     4141 atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt
     4201 ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg atcagtatgg
     4261 acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct
     4321 atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc
     4381 gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg
     4441 ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta
     4501 cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca
     4561 attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag
     4621 cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt
     4681 tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa
     4741 ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt
     4801 gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt
     4861 ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag
     4921 gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct
     4981 tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc
     5041 ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca
     5101 aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca
     5161 aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag
     5221 taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa
     5281 tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg
     5341 tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa
     5401 aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa
     5461 agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg
     5521 aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat
     5581 cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt
     5641 gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa
     5701 gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg
     5761 tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt
     5821 tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc
     5881 aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat
     5941 ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt
     6001 atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg
     6061 attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa
     6121 tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc
     6181 agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg
     6241 ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg
     6301 ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa
     6361 attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt
     6421 gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt
     6481 gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa
     6541 aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt tggaggaata
     6601 gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc
     6661 tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg
     6721 aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat
     6781 caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc
     6841 cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat
     6901 gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac
     6961 acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt
     7021 gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga
     7081 acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct
     7141 ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg
     7201 gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta
     7261 tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc
     7321 gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg
     7381 agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta
     7441 tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga
     7501 gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg
     7561 tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac
     7621 aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat
     7681 tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga
     7741 aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg
     7801 catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt
     7861 agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa
     7921 tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca
     7981 atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa
     8041 tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata
     8101 aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta
     8161 cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac
     8221 caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta
     8281 gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct
     8341 tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag
     8401 cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc
     8461 attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa
     8521 ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt
     8581 actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa
     8641 acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa
     8701 aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga
     8761 agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat
     8821 gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag
     8881 aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg
     8941 tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa
     9001 atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc
     9061 gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca
     9121 acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag
     9181 atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac
     9241 aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag
     9301 agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta
     9361 aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat
     9421 caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg
     9481 caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag
     9541 agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa
     9601 gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca
     9661 attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa
     9721 cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc
     9781 aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg
     9841 gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat
     9901 cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca
     9961 tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa
    10021 gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca
    10081 ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg
    10141 atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat
    10201 taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg
    10261 gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc
    10321 tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga
    10381 tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag
    10441 aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt
    10501 tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg
    10561 ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga
    10621 ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa
    10681 aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat
    10741 gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat
    10801 catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg
    10861 gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt
    10921 cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag
    10981 catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt
    11041 tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt
    11101 ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg
    11161 tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta
    11221 tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt
    11281 agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg
    11341 tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag
    11401 tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat
    11461 tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc
    11521 ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt
    11581 tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc
    11641 actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt
    11701 gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa
    11761 gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa
    11821 gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa
    11881 cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg
    11941 gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt
    12001 tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca
    12061 gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca
    12121 gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc
    12181 ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt
    12241 gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa
    12301 gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc
    12361 gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca
    12421 ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca
    12481 tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat
    12541 ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat
    12601 gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt
    12661 gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg
    12721 atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt
    12781 atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact
    12841 aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc
    12901 ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg
    12961 gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa
    13021 gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca
    13081 agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt
    13141 atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact
    13201 gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa
    13261 aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta
    13321 aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact
    13381 attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga
    13441 agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta
    13501 tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa
    13561 ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc
    13621 gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc
    13681 tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc
    13741 gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag
    13801 gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt
    13861 agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct
    13921 agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt
    13981 ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga
    14041 gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga
    14101 acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt
    14161 tttattactc ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa
    14221 aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact
    14281 ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac
    14341 attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag
    14401 aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt
    14461 ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt
    14521 ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga
    14581 agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa
    14641 aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat
    14701 gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct
    14761 tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc
    14821 tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag
    14881 aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg
    14941 ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata
    15001 atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt
    15061 ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga
    15121 ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca
    15181 aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga
    15241 aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta
    15301 tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt
    15361 cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt
    15421 agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac
    15481 cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga
    15541 agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac
    15601 tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga
    15661 aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg
    15721 taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat
    15781 cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg
    15841 cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg
    15901 acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat
    15961 cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt
    16021 tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata
    16081 ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc
    16141 aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg
    16201 agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa
    16261 aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa
    16321 agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc
    16381 acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc
    16441 tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt
    16501 atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt
    16561 ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc
    16621 ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt
    16681 cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg
    16741 ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa
    16801 aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt
    16861 tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc
    16921 gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca
    16981 gtgatcaata gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga
    17041 ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt
    17101 ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt
    17161 acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt
    17221 tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt
    17281 cgacaaatgt ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct
    17341 ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa
    17401 aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct
    17461 attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag
    17521 atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg
    17581 gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt
    17641 tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat
    17701 cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat
    17761 acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa
    17821 aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa
    17881 ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg
    17941 atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac
    18001 ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa
    18061 aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt
    18121 gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg
    18181 cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt
    18241 ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa
    18301 gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa
    18361 ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta
    18421 gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa
    18481 gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat
    18541 gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct
    18601 gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca
    18661 gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt
    18721 gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg
    18781 gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa
    18841 aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga
    18901 caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca
    18961 gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct
    19021 ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact
    19081 tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca
    19141 gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt
    19201 ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg
    19261 tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa
    19321 tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa
    19381 ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa
    19441 ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag
    19501 gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat
    19561 ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat
    19621 cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta
    19681 gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa
    19741 gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt
    19801 ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca
    19861 cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac
    19921 aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct
    19981 catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag
    20041 ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa
    20101 tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt
    20161 acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt taacaattat
    20221 ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg
    20281 cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga
    20341 tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt
    20401 agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc
    20461 ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt
    20521 attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac
    20581 cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac
    20641 accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc
    20701 ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc
    20761 gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta
    20821 agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag
    20881 cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc
    20941 ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt
    21001 catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc
    21061 cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt
    21121 atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct
    21181 ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag
    21241 gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa
    21301 cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat
    21361 gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt
    21421 atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc
    21481 cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc
    21541 atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag
    21601 tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact
    21661 ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg
    21721 aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc
    21781 ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg
    21841 tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag
    21901 aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt
    21961 aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt
    22021 taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg
    22081 caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta
    22141 gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct
    22201 gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt
    22261 ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag
    22321 ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc
    22381 ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa
    22441 ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa
    22501 aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc
    22561 attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat
    22621 tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta
    22681 aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc
    22741 ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag
    22801 aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt
    22861 gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc
    22921 taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac
    22981 gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta
    23041 tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg
    23101 atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc
    23161 gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt
    23221 ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct
    23281 gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta
    23341 attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat
    23401 attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc
    23461 acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga
    23521 cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc
    23581 aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg
    23641 cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa
    23701 atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc
    23761 tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg
    23821 tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat
    23881 tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga
    23941 tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt
    24001 tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct
    24061 tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt
    24121 tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct
    24181 gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca
    24241 cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc
    24301 attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga
    24361 gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt
    24421 cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga
    24481 gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga
    24541 acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga
    24601 actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt
    24661 atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga
    24721 tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac
    24781 agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca
    24841 aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa
    24901 aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat
    24961 cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga
    25021 tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa
    25081 tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt
    25141 attttgacta cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc
    25201 attctttttc tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa
    25261 acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc
    25321 aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg
    25381 ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa
    25441 gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta
    25501 tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca
    25561 gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag
    25621 gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat
    25681 gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc
    25741 tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt
    25801 aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga
    25861 ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct
    25921 gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt
    25981 aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt
    26041 agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc
    26101 ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa
    26161 ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca
    26221 agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg
    26281 gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta
    26341 tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca
    26401 ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc
    26461 atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta
    26521 tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag
    26581 gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta
    26641 tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc
    26701 cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata
    26761 ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca
    26821 tgaatctggt aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca
    26881 aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa
    26941 ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga
    27001 tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat
    27061 aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt
    27121 caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg
    27181 tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca
    27241 ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc
    27301 tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc
    27361 gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt
    27421 tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag
    27481 aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt
    27541 atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg
    27601 ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt
    27661 tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag
    27721 taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct
    27781 aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag
    27841 tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc
    27901 aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga
    27961 tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca
    28021 tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag
    28081 aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat
    28141 cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga
    28201 catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg
    28261 acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc
    28321 attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg
    28381 gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg
    28441 acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga
    28501 aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac
    28561 attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc
    28621 ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg
    28681 tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca
    28741 ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag
    28801 tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat
    28861 ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg
    28921 gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt
    28981 atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag
    29041 gttttctcgg attgattccg attttactag gtattattag cggatacatc gtagccttgc
    29101 tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact
    29161 ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg
    29221 cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac
    29281 taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat
    29341 tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca
    29401 ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat
    29461 ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac
    29521 cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga
    29581 ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta
    29641 tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa
    29701 tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa
    29761 gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc
    29821 atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc
    29881 aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc
    29941 tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag
    30001 ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat
    30061 acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg
    30121 atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg
    30181 gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg
    30241 aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc
    30301 gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag
    30361 gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg
    30421 aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg
    30481 gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg
    30541 cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag
    30601 aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg
    30661 gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg
    30721 aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga
    30781 cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct
    30841 cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag
    30901 tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg
    30961 ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc
    31021 ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag
    31081 ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg
    31141 atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc
    31201 aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag
    31261 tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg
    31321 cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag
    31381 atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag
    31441 aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt
    31501 caccccatca tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa
    31561 tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg
    31621 gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa
    31681 gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata
    31741 ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta
    31801 aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg
    31861 tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag
    31921 gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca
    31981 acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa
    32041 gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc
    32101 gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt
    32161 caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat
    32221 gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca
    32281 aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata
    32341 tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct
    32401 agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt
    32461 ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca
    32521 ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt
    32581 aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg
    32641 ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa
    32701 gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt
    32761 caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga
    32821 ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat
    32881 catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta
    32941 gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg
    33001 cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt
    33061 atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa
    33121 gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta
    33181 cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa
    33241 ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc
    33301 gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc
    33361 aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt
    33421 agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga
    33481 tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat
    33541 cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt
    33601 tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt
    33661 acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg
    33721 aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc
    33781 aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat
    33841 cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc
    33901 tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga
    33961 tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc
    34021 gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg
    34081 attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc
    34141 tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg
    34201 agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa
    34261 tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga
    34321 acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca
    34381 agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt
    34441 agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat
    34501 cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa
    34561 cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca
    34621 gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga
    34681 attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg
    34741 atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt
    34801 tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc
    34861 aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt
    34921 atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat
    34981 aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc
    35041 tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga
    35101 tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac
    35161 aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc
    35221 aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga
    35281 ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt
    35341 gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg
    35401 gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc
    35461 acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc
    35521 attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt
    35581 ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac
    35641 aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact
    35701 tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt
    35761 ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg
    35821 agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc
    35881 tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga
    35941 agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa
    36001 acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc
    36061 tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt
    36121 tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag
    36181 agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt
    36241 tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa
    36301 gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc
    36361 gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta
    36421 aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta
    36481 ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt
    36541 tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta
    36601 gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa
    36661 gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta
    36721 gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat
    36781 tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac
    36841 ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt
    36901 tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa
    36961 aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc
    37021 gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga
    37081 ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc
    37141 ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag
    37201 aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc
    37261 caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga
    37321 atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat
    37381 tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact
    37441 atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca
    37501 tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt
    37561 ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc
    37621 caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt
    37681 ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt
    37741 tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga
    37801 ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag
    37861 tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga
    37921 atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg
    37981 aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat
    38041 gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga
    38101 agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt
    38161 gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt
    38221 tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc
    38281 aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc
    38341 ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc
    38401 tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt
    38461 ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg
    38521 tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt
    38581 ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt
    38641 cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac
    38701 tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa
    38761 aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa
    38821 tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg
    38881 aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat
    38941 caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca
    39001 tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa
    39061 agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt
    39121 gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag
    39181 agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa
    39241 agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat
    39301 tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg
    39361 gaaaaaagat cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga
    39421 tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc
    39481 atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa
    39541 ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt
    39601 atatatagat ttt
//
LOCUS       DACDCV010000015        38414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDCV010000015 DACDCV010000000
VERSION     DACDCV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.015"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..1140)
                     /locus_tag="IV041_000794"
     CDS             complement(34..1140)
                     /locus_tag="IV041_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8381608.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1350..2351
                     /gene="ccpA"
                     /locus_tag="IV041_000795"
     CDS             1350..2351
                     /gene="ccpA"
                     /locus_tag="IV041_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8381609.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2688..5033)
                     /locus_tag="IV041_000796"
     CDS             complement(2688..5033)
                     /locus_tag="IV041_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8381610.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5312..6214
                     /locus_tag="IV041_000797"
     CDS             5312..6214
                     /locus_tag="IV041_000797"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8381611.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6427..8202
                     /locus_tag="IV041_000798"
     CDS             6427..8202
                     /locus_tag="IV041_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8381612.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8268..9854)
                     /locus_tag="IV041_000799"
     CDS             complement(8268..9854)
                     /locus_tag="IV041_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8381613.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10105..10329
                     /locus_tag="IV041_000800"
     CDS             10105..10329
                     /locus_tag="IV041_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8381614.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10471..12222
                     /locus_tag="IV041_000801"
     CDS             10471..12222
                     /locus_tag="IV041_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381615.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12222..14006
                     /locus_tag="IV041_000802"
     CDS             12222..14006
                     /locus_tag="IV041_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381616.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14332..15183
                     /locus_tag="IV041_000803"
     CDS             14332..15183
                     /locus_tag="IV041_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8381617.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15237..16988
                     /locus_tag="IV041_000804"
     CDS             15237..16988
                     /locus_tag="IV041_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381618.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16992..17162
                     /locus_tag="IV041_000805"
     CDS             16992..17162
                     /locus_tag="IV041_000805"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381619.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17202..18104)
                     /locus_tag="IV041_000806"
     CDS             complement(17202..18104)
                     /locus_tag="IV041_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8381620.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18335..19738
                     /locus_tag="IV041_000807"
     CDS             18335..19738
                     /locus_tag="IV041_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8381621.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19738..21171
                     /gene="ascB"
                     /locus_tag="IV041_000808"
     CDS             19738..21171
                     /gene="ascB"
                     /locus_tag="IV041_000808"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8381622.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21316..22242
                     /gene="trxB"
                     /locus_tag="IV041_000809"
     CDS             21316..22242
                     /gene="trxB"
                     /locus_tag="IV041_000809"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8381623.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22456..24183
                     /locus_tag="IV041_000810"
     CDS             22456..24183
                     /locus_tag="IV041_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8381624.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24255..24548
                     /locus_tag="IV041_000811"
     CDS             24255..24548
                     /locus_tag="IV041_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8381625.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24640..25500)
                     /locus_tag="IV041_000812"
     CDS             complement(24640..25500)
                     /locus_tag="IV041_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8381626.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25592..26020
                     /locus_tag="IV041_000813"
     CDS             25592..26020
                     /locus_tag="IV041_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8381627.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26182..26358
                     /locus_tag="IV041_000814"
     CDS             26182..26358
                     /locus_tag="IV041_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8381628.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26385..26831
                     /locus_tag="IV041_000815"
     CDS             26385..26831
                     /locus_tag="IV041_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8381629.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(26985..28364)
                     /gene="brnQ"
                     /locus_tag="IV041_000816"
     CDS             complement(26985..28364)
                     /gene="brnQ"
                     /locus_tag="IV041_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8381630.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            29070..30041
                     /locus_tag="IV041_000817"
     CDS             29070..30041
                     /locus_tag="IV041_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8381631.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30065..32257
                     /locus_tag="IV041_000818"
     CDS             30065..32257
                     /locus_tag="IV041_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8381632.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            32274..32750
                     /gene="ybeY"
                     /locus_tag="IV041_000819"
     CDS             32274..32750
                     /gene="ybeY"
                     /locus_tag="IV041_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8381633.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            32728..33129
                     /locus_tag="IV041_000820"
     CDS             32728..33129
                     /locus_tag="IV041_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8381634.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            33144..34043
                     /locus_tag="IV041_000821"
     CDS             33144..34043
                     /locus_tag="IV041_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8381635.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            34186..34998
                     /gene="recO"
                     /locus_tag="IV041_000822"
     CDS             34186..34998
                     /gene="recO"
                     /locus_tag="IV041_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8381636.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            35382..36299
                     /gene="glyQ"
                     /locus_tag="IV041_000823"
     CDS             35382..36299
                     /gene="glyQ"
                     /locus_tag="IV041_000823"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8381637.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            36301..38376
                     /locus_tag="IV041_000824"
     CDS             36301..38376
                     /locus_tag="IV041_000824"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8381638.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        12420 a         6903 c         8127 g        10964 t
ORIGIN      
        1 gtgggagcgg actagctctc acctttttta ctcttatttt tctaaaacaa gtaattcttt
       61 tggtgtagtc gtgaatggca cacagccatc gcttgtcaca tatacacagt cttcgatacg
      121 tacccctact ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc
      181 gattactaag tcatttcctt ctacaaggga agggaattcg tggacggttg ttccgatccc
      241 gtgacctaaa cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc
      301 gatttgatca agttcacctg ctgtcacacc tggacggacg gcttcttgtg cggcaagctg
      361 tgcttcaaga gtgatgttgt agattttttc ttggaagtcg gttggttttt gataagcaat
      421 tgtacgagta gcatcactgc aatagccatt ccaaacaacg ccaagatcga ataacacaag
      481 ttcattcggt gaaatcttcg tatttcctgg tgtaccatga gggcttgcag catttgctcc
      541 tgcaaggact aatgtgtcga aagacatttg tgaaacacct tgttttttca attggtattc
      601 aatttctgca atgatcgctt gttcagtcac gccttctttc actgctttaa agccaatctc
      661 aaatgcaaca tcggcccatt taccagcttc gatcagtttg tctttttcct ctagagtttt
      721 gatcagttgt agacgttgga tgatggaagt tagatcagtg taagaatccg ttcccgtcaa
      781 gatatcagag atagcatcga agcgctcgac agtcaaggcg ttcttttcaa tccctacatt
      841 gccaggctga ccatagcgag cacgaagttc ttttccgatg atctcccaag ggttttcgct
      901 gtctaaatag ccacggacat catacgccca actactgttt tgagcatctt caacttccaa
      961 agcaggagtg aaaaggaaag gatcttgttc aagagggata aaaagagcta gtacacgctc
     1021 atgaggatca ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc
     1081 caattcgact tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat
     1141 ttggcaacct actttctaaa ttaattttgt attcaataat tgtaccactg atttattaaa
     1201 agaccaacgt tctaaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg
     1261 ttgcaattct tgagattatt tgatattcta ttttagaaat gtaagacgta ttttcagaaa
     1321 acatataaaa tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg
     1381 tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa
     1441 acgttaaacc agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc
     1501 caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc
     1561 ctgatgtcag caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa
     1621 tgtacaaata caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg
     1681 tattgaataa cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca
     1741 cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta
     1801 ttgatccaga tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg
     1861 cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga
     1921 tcgatcccat caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata
     1981 acctaccata tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat
     2041 ctctagtcga tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat
     2101 tagccatcgg tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg
     2161 aaatcatcac aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta
     2221 tcacacaacc attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga
     2281 ataaagaaga agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat
     2341 ctacaaaata ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt
     2401 tacaaggagc aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt
     2461 cggagttggc aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat
     2521 aatccttttt ttgtgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat
     2581 aaaacaatcc gtgtatagat gctgcatttt gttcaccacc atcaaaaaaa taagggactg
     2641 tgacaatact attgtcacaa tcccttatta ctgtgtattc tattaaatca atcatctttc
     2701 tcatctgatt ctttagttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa
     2761 tctgtatagt tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca
     2821 tttttcgccc atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca
     2881 gtaacttttc caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat
     2941 gataattcga atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg
     3001 atcagattgg caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg
     3061 tcatcgtgcc ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa
     3121 tctcggtatt cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg
     3181 tttaagcttg agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactacgc
     3241 atcatgtcat tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg
     3301 tgtttataaa ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca
     3361 tagacgccgc cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct
     3421 actgtaccta aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt
     3481 gccaagtagt gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat
     3541 aagtgataag ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg
     3601 ttcgatcctt cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa
     3661 gttgttgcat aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt
     3721 ccataaacaa gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg
     3781 ttattttgat tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg
     3841 ttatccatca atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat
     3901 ccataatttt caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg
     3961 attgtatatc ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc
     4021 gatgaatcag acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt
     4081 gcttcattca tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt
     4141 ggtagaaagt cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt
     4201 tggccttcac gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga
     4261 tcttctttta gcgcgcctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat
     4321 ccagcgatat aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct
     4381 tttgcagctt cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag
     4441 acattcaaat acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa
     4501 agaatgatct cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg
     4561 actaattgct gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact
     4621 aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct
     4681 gtgctgataa ttgcagtttt caataaaggt gaaatcttat cagatgaaat agtcgtgcgc
     4741 atcagatctg tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt
     4801 tctgtaatgt ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg
     4861 acgagatagg caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga
     4921 agaacagtaa taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac
     4981 catttttctt tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc
     5041 acctcaacta atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca
     5101 aggctgtttt tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt
     5161 attataaaag agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg
     5221 gcaaagaaaa atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag
     5281 taacaattgc ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag
     5341 cggaaagaaa atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt
     5401 tatgatctat acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat
     5461 tgatgccgga aaagcgtaca tggcagaaaa aggtctaaca aatatcgtcg ttcatgcccc
     5521 ttatatcatt aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt
     5581 tctgagagaa gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc
     5641 aggtgctcat gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa
     5701 tgaagttttg acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg
     5761 tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa
     5821 tgaaaaatta tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa
     5881 agaagacttt tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa
     5941 agtactccat atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa
     6001 tattggtttt ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt
     6061 tgacaatatt tcgaaaatct tggaaactcc ctatgtaggc aaagataaaa agaaagctta
     6121 cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa acattcgatc cagaagtgtt
     6181 tcctgagtta ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa
     6241 aggaccaggc acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag
     6301 tctttttcat catgcatctt ctcccccctc tttttaaatt tagacaaaca aggtatactg
     6361 gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac
     6421 gaaaagatgc tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac
     6481 tttattcttg gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca
     6541 atctatggaa cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa
     6601 aatattttgg acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc
     6661 tcctacagct tatttttagt acttgcacat aaactgcatc tttcttatgc agttttgttc
     6721 ggcatgttca gtatttttgc cagtatattg gttgcactcg cgtttaaacc gattgtgaag
     6781 aacaagtatc ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca
     6841 catgaatatt ccactcgaac atatcgtaat gccctcgtga ttccggctgt tgagatcatt
     6901 atcgcttgtt tattagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca
     6961 tggtttttag ggctttctgt cgcttttcct ttcttttggt tcattcgcga agattctatc
     7021 tggcttcttc cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg
     7081 caagactttt ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa
     7141 cgggaatggc tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat
     7201 caatggatcg aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcc
     7261 agtggagaat ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg
     7321 aataaagaaa acagtgagat atgggtctca catgctgcac taaataaagc attagccgca
     7381 tctcctacat tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg
     7441 actcaaggtg gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat
     7501 gtggtaagcg aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa
     7561 aaagtcaacc aagaattaga agaagcctat cacaagggaa caatcaaaaa gaaaaaagag
     7621 ttctatctga tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat
     7681 ttccttcaat caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat
     7741 tatagtttcg gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta
     7801 atggagaatt ggatcgatag taatgaaccg agaacaccta atttccaatt aacaaaatca
     7861 gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt
     7921 gcttcaatag cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt
     7981 gcctattata gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt
     8041 cttttcggtg tctcatggtt ttgttcttat gcgccagaaa aaaaggacta ttttttaagt
     8101 gtctacactg gtgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt
     8161 ttcagtaaat taaatctgaa aaaagcttta tcttctaaat aaaaagaccc ttggtagcta
     8221 gatttttaac ctaactatca agggtctttg tgtggaaaaa tcagttttta ttcgatttct
     8281 tctaccgttt cctgcaaaag ctgttcatat tttttgtttc ctttatataa atcaccaatc
     8341 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat
     8401 gctagttgag cggaagtggc ttgctcgccg aagaaacttt cgatctgtcc tggtaatcct
     8461 cgtaagttca agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat
     8521 gaatttttaa atcggctacc catctccgct tggctgttgg tcatcatcag taaaggaatc
     8581 atcgaaaacg gcaacgcaaa agccaagaac acctgggatt cattcattaa gttattcaaa
     8641 gcggcatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
     8701 atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg
     8761 aatccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
     8821 gcaagaagtg ctacagcaaa taaagtggac aagataccag attttgctac agcaatcaaa
     8881 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
     8941 tcttggacag aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact
     9001 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaaacgtac agcttgtgct
     9061 acatcttctt cgttttggtg atcgatcttc cgagtttgtg aaactgctga atgaagatat
     9121 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg
     9181 ccgccgatga ccggtgagtc tgagaatgta tgactgttag ggataaagcc agcaaataaa
     9241 ctgtgccaat ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa
     9301 attaagcaga caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga
     9361 acgaaaacat ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag
     9421 tagagggcga ttgctgcacc aatgacttct gcaatatctg ttgccataat ggctaattca
     9481 gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc gtgctcggat tgcctgtgcc
     9541 aaatccattt ggctcacgat tcctaatttt gctgccatat actgtaaaag catagcaatc
     9601 agactagaca tcaaaataac tgacatcaat aaatattgga aattttgtcc tccggtgatc
     9661 gaagtagacc agttacccgg gtccatatac ccaactgcta ctaacgcacc aggtccagag
     9721 taagcaagaa gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact
     9781 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttattg
     9841 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
     9901 aaaaagaaaa aattaaagaa aaagattcgg tacgcctaat tttttttcat ggcgcgtctt
     9961 gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac cactattttt tgcatgaaac
    10021 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt
    10081 cggaaataga aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt
    10141 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
    10201 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
    10261 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
    10321 aaaaaatagg ttctgaccca acgacttttt acgggctgat tttttcagtc cgttttttat
    10381 ttcgtaattt gttagactat tcagattatt cagcaaaaaa gacaagcaca gacaatccta
    10441 ggctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggctggttt
    10501 tttcgtcagg aaaagaaaca ttatttaatt ggggttttct cgctattcgc agtggccttg
    10561 gtacagttag ttcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat
    10621 ttatcaataa agatcatttt cgggtggatc ttcctattga ttatcgctgc ttttttacaa
    10681 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg attggagaaa
    10741 gaacttcggc agcagttatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
    10801 tatcggactg gggatttgat ggctcacgca acgaatgatt tgaatgcgat ccagaatgta
    10861 gcaggagctg gtatcttgac ctttgcagat tcagtgatta cgggcggaac aacgatcatt
    10921 gcgatgattt tgtttattga ttggcgctta acactgattg cgctgattcc tctgcctttg
    10981 ctggccgtca cttcacgagt gctggggtca aaactccatg atgcttttcg tgattcgcaa
    11041 gaagcttttt ctagtatcaa tgataagaca caagaaagta tcactgggat caaagtcatt
    11101 aaaacatttg gacaagaaaa agaagatcta gcggattttg aagaaaagat tgaagatgca
    11161 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
    11221 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
    11281 acgatctcga tcgggcagat ggtctcattt attagttata tcggtatgct tgtttggccg
    11341 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
    11401 gtcaatgaac ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagacc
    11461 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta attatccgaa agaagaatca
    11521 ccagcattag aacaaatcaa gtttgcgatc caagaaggag aaacattagg tattgttggg
    11581 aaaactggtg cgggaaaaac gacgatcatc aaattactga tgcgtgaata cgatgattat
    11641 caagggaaaa tccaatttgg cggacgttcg atcaaagaat attcattgaa tgctttatta
    11701 ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt cgatgaccat tcgagataat
    11761 atccgttttt ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca
    11821 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
    11881 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
    11941 aatcctgaat tattaatatt agatgatgca ctctctgcag tagatgccaa aacagaagaa
    12001 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caactatcat tgcagctcac
    12061 cgtttaagta gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt
    12121 gaacgaggaa cccatcatga gctgcttgca ctaggcggct ggtatgaaca aatgtgggag
    12181 aaacagcaat tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat
    12241 cggaatggac aaaatcgatt ccacttaaag aacagaaaaa gatcatcaaa cgactgatga
    12301 agttcgccaa accttttaga agaacgttca tcgttgctat cttatttgcg tttgctttgt
    12361 ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta
    12421 aacagtcggc tacgacacaa gttattttgt tttttgctgg tatgtactta tttggagtca
    12481 tagtaaaaag tatcatttgg tttttccaat ggtatttgta ttccatggcc tcattgaaga
    12541 cctatcagta tattagggta aaattatttg aaaagctgca tacactaggt atgcgttatt
    12601 ttgatcagac accggcagga tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat
    12661 ttgaattttg gtacgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcat
    12721 tccttgccat gtttcaaatc aatggagaaa ttgccttgtg gactttggtg tttttgccga
    12781 ttttaggaat cgtcatctgg tattaccaga aattcagttc tcgtatttac cgaaatatgc
    12841 gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta
    12901 tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt
    12961 atttagacac gcgttatgcg atgatcaaaa cgaattcgat tctgttaagt ccgatcatca
    13021 attttctata tgcattggcg attgcgttgg tgttgacttt gtttgggatc gatgcgttgc
    13081 attctcccgt agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta
    13141 atccaatgtc tcaaatgatg gactttttga gcatcttcac agatgggatg gttgctggta
    13201 gtcgtatctt gaagattatg gatacggaag aatatgcacc acaacaaaac cctggagcag
    13261 atcaaatgat tacttcaggt aagattgagt ttcgcaatgt aagcttttct tatgatggag
    13321 agcacaatgt attaaacaat atctcttttg ttgctaatcc gggagagacc gttgcattag
    13381 tgggacacac cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat
    13441 tttatgaagg tcagatattg attgatgatc atgacattcg agaatatcca atggaagagt
    13501 tacgtaagaa aatggggctg gtcttgcaag atgctttctt gttttatggg gatatcgcag
    13561 gaaatattcg gatgatgaat ccagctatca cagacgaaca gatacgccat gcggctgagt
    13621 ttgttcaagc aaacaaattc atcgatgaat taccaaaagg atatcactca aaagtgatcg
    13681 aacatggtgc cagctattct agtggacaac gacagctgat cacttttgcg cgaacaatcg
    13741 tgactgatcc taaaattcta gtattggatg aagcaacagc aaatatcgat actgaaacag
    13801 aaggattgat ccaagaagga ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg
    13861 cccatcggct gtcaacgatt cgagatgcag aattgatttt agttttagac aaaggtcata
    13921 tagtagaacg cgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt
    13981 acaaactgca aagcagtggt atataaaaga tgttgtgaca gtagaatttt gacctaagca
    14041 ctagagtgaa aataaaacat agcttctcat atttttcatc tgtgagagtt agtgtcgtag
    14101 tcaccagatt taaaaaaaga ttcgacagct agtcagaagt gcctaatagc cactaagaaa
    14161 gcgttgacaa aagcatttgc tttcgataat ataaagaaaa cgatacaact tggatagtga
    14221 ttgcctgatg caagctaaac ctgatgagtc tcccatggtt tcatggacgg ctttttgggt
    14281 ttttcttttt gttttggata cagccctata catcatggaa aggagcaaaa catgctgatt
    14341 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
    14401 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
    14461 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
    14521 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
    14581 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
    14641 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
    14701 caactatttc ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa
    14761 taccacatca agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca
    14821 caaggcattc aaagccagca agacaatacc tacctgttta ctataacaat acaggatatt
    14881 ctaaatatcg tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt
    14941 cgattcgtca cacacttacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa
    15001 gtgggagaag tttctgagga attgtttcag gttattcaaa aaaaatataa ggatacgttt
    15061 ggttgcgttg agaaaatagc agacttttta gccgagacgt tcaactacca gatgtccacg
    15121 gacgaaaaaa tgtatttaac gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa
    15181 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
    15241 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
    15301 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
    15361 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
    15421 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc attgggaatc
    15481 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
    15541 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
    15601 agccattcct tgctatccta gctgctggcg ggatgattcg tggactgaca gcattccttg
    15661 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
    15721 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
    15781 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
    15841 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
    15901 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
    15961 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
    16021 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
    16081 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
    16141 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
    16201 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
    16261 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
    16321 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
    16381 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
    16441 cattaccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
    16501 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
    16561 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
    16621 ccattgcgat ggtggtatcc tttacgctga cttttttctt ttggaaggat gaagcaggtg
    16681 aaacagatga tatacagaaa aaatcaggta aagaaattgt aaaaacaccg atacaaggac
    16741 aaatcgctcc actaaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
    16801 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga
    16861 ccctgttccc gacgaaacag gcaattggaa tggtttctga aacagggctg gaattgctga
    16921 ttcatgtcgg attggatact gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc
    16981 aaggataaag agtgaaacaa ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa
    17041 aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata
    17101 ttttggaaaa ccaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct
    17161 gaacggttag gtgaagaatg gactaggctt aactattctt tttattccac ttctttattt
    17221 tcaaaattga aaggctcttt catgatggct gagctgatag ggcgtttatc gaatttttga
    17281 ccgacagaaa ttagatggac taaattctgt tgataatttt ctgcaaaaac acaactatat
    17341 aacacgtcta gaagataaca aattgatgta ttgatcgtaa aattaccaat ttttgaatac
    17401 agtcgctcgc gtgtcgtaag tcgcaaagcg gctgtactca ttgcagatag cgttgaatca
    17461 ccgatactgg tcaaagctaa aattggaaca tgattttgtt tcaatatttc tgcggtacga
    17521 aggatcacat tgttttcacc cgtataagaa atcagaatcc cgcacatatc actcgtgcaa
    17581 ttgtacgctt catagaatga ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg
    17641 cgcttcattt tcagcatgaa atcttgagaa atcaaagtat tcgagttact ggcgaaaatc
    17701 acgatttttt ttgcttgaag cattagttgt ttggcttttt gcagttggtc atgatgaatg
    17761 agagaaagtg tatcatgaat cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc
    17821 ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca
    17881 agataggctg tttcagctaa aaaagcatta cgaaaactga cccagccatc aaaacccatt
    17941 ttttttgcta cacggatgag tgtagagggg tggacataac atcgctcagc aatcgcttgg
    18001 atggtcattt cgccaagtgc tggagaatcg cttaagagaa actggatcac ttcattttct
    18061 gaaggagaaa aggagatttc tttgattttt tcacgtaata acatgtagat accttcttct
    18121 tattttttta cgtaaattat aacttttttg aaaatgattt gtaaacggat ttatactata
    18181 taacttaaaa tagacatttt ttgacaagat atttacaaaa atccactaga aaacagtaaa
    18241 ttttgtaaat gttactggaa agaaacagat ttctattgtt tgttttgttt acaaaaaaga
    18301 taatgaaagt gtaaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
    18361 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
    18421 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    18481 gatcgttagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaagatcta
    18541 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    18601 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
    18661 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtcgt
    18721 tttggcaatc gttgaggaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc
    18781 gaacccaagt gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    18841 tggattatgc aatgtgccag tgatggcgat gatgacagaa ccagcaatgc ttggcaagaa
    18901 aaaagaagag ctgatctaca agtttgccgg tctaaatcat ttccattggc acaaagtagc
    18961 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagagaa
    19021 tggattaccg aaaaatatct ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa
    19081 catgatccct tgtggctacc accgctatta ctatcgtgaa gaagaaatgc tggaacatgc
    19141 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    19201 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
    19261 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
    19321 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
    19381 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
    19441 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    19501 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
    19561 acttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
    19621 gcagtattta ccgcaattca aggagacaat cagccgatta aaagaagaag gaatcgaagt
    19681 tcaagatgaa ctggcaagag aattatcagg taatacacat ataatggagg aagtataatg
    19741 ggattcagaa aagatttttt atggggcggt gctacagcag ccaatcaatg tgagggcggt
    19801 tatgatgaag gtggacgcgg actagcaaac gtagatgttg tgccaatcgg aaaagaccgt
    19861 tttcctgtta tcaccggaaa gaaaaaaatg tttgaatttg atgaggatca tttttatcca
    19921 gcaaaaattg caatcgatat gtatcatcgt tataaagaag atattgcttt gtttgcagaa
    19981 atggggttca agacttatcg aatgtccatc gcctggagtc gtatttttcc aatgggggat
    20041 gagaaagagc cgaatgaagc tggcttgaaa ttttatgagg atgcctttaa agaatgccaa
    20101 aagcacggaa ttgagccact tgtgacgatt acgcattttg attgtccaat gcatttgatc
    20161 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga
    20221 gtcatcttca atcgctataa aggacttgtc aaatattggc tgacattcaa tgagatcaat
    20281 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaaaaagg agaaaatgaa
    20341 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    20401 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    20461 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
    20521 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    20581 gagcgaaatg gcattcagat cgaaatggaa ccaggggatg ccgcattgct gaaagaacat
    20641 actgttgatt ttatctcttt ttcttattat gcttcccgag tttcgactac cgacaacgaa
    20701 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctgcaagca
    20761 agtgaatggg ggtggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    20821 gatcgttatc aaaaaccatt gtttattgta gaaaacggct taggtgcagt cgatacgcca
    20881 gatgaaaatg gttatgtgga agatgattac cgaatcgatt acctggcagc gcatatccag
    20941 gcgatgaagg atgcagtaga actagacgga gtcgatctat taggctatac gacttgggga
    21001 tgtattgatt tagtatcagc tggtaccggt gagatgaaga aaaggtacgg attcatttat
    21061 gtcgatcgtg ataaccaagg aaatggcaca ttagcccgtt caaagaaaaa atcattcaac
    21121 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt
    21181 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    21241 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    21301 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
    21361 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggtgcccct
    21421 ggcggccaaa tgaacaatac ggcagaaatc gaaaattatc ctggattcga ttcgatcatg
    21481 ggccccgatc tagcttataa gatgtatgaa agtgtctcaa gatttgggac agaaaatgct
    21541 tatggaatcg ttcaagatat caaggatcac ggcagccata aagaagtcat ctgtgaagat
    21601 aaaagttacg aaggaaaaac agtcattatc gcaacaggat gtatccaccg taaattaggc
    21661 gtacctggcg aggaagaatt tgctggacga ggcgtttctt actgtgcagt ctgtgatggt
    21721 gcgtttttcc gtaacaaacg tctagtagtc atcgggggtg gggactcagc agtagaagaa
    21781 gccatctatt tgacacaatt tgcttctgaa gtagtgatcg tccaccgtag agatgaatta
    21841 cgtgcacaaa agatcatcca agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg
    21901 gatacagtcg ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt
    21961 aatgtcaaaa ccggcaatga atttgattta gcagcagacg gagtattcat ctatgttgga
    22021 ctggatccat taactgcacc ttttagagaa gcaggtttga cgaacgaagc tggctggatt
    22081 ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt
    22141 gaaaaagatt tgcgacaaat cactacagct gttggtgacg gcggtattgc tggacaacaa
    22201 gtgtataaat acttggagtc tttagaagaa acaacagcat aagtttaaaa agagtagaag
    22261 aacgggttca attgttcttt tactcttttt tttcgcgtga aatttactga atctgattta
    22321 tgcgaacacg tccaacacct tttgataaaa gaatcctttc ttgatttaag cgttttttgg
    22381 tcctcatact ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
    22441 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
    22501 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
    22561 gatgcttttt atgcaccgtt ggaattcggt actgcaggta tgcggggaat cttaggacct
    22621 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact agcacgtttc
    22681 atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
    22741 catcagtcac cagaatttgc aatggaagca gccaaaacac tagcacatca caatatcccg
    22801 tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg
    22861 aagacgtttg cgggaatcat ggtaaccgca tctcacaacc cagcggctta caatggatat
    22921 aaagtctacg gagaagatgg cggacaaatg cctcctgcag acgcagatgc attgacgaaa
    22981 tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga agaagctaaa
    23041 catagcgggt tgatcacgat catcggagaa gaagtggacg ctgcttatct agaagaaatc
    23101 aaagcagtga cgattgatcg tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg
    23161 tacacgccac ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga
    23221 ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag atccagattt cactacggtc
    23281 aaatcgccaa atccagaaga acattcagct tttgaatatg ctatacgact tggagaaaaa
    23341 gaaggagctg acttgctgat tgcaacagat cctgatgcag accgtttagg cgcagcggtt
    23401 cgtttgccag atggttctta tgaagtattg actgggaacc aaatcggagc attgtttgct
    23461 caatatattt tggaagcaca tcaatcaaga ggcactttac cagaaaacgc agcggtattg
    23521 aaatcaatcg tttccagcga attgcctaca gcaattgcgg aacactacgg tgctaccatg
    23581 ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa tccaacaatt tgaagaagac
    23641 cattcacata cttttatgtt tggattcgaa gaaagttatg gttatttagt taaacctttt
    23701 gttcgcgata aagacgcgat ccaagcattg cttttattag cagaagtagc agcttactat
    23761 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctat
    23821 ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
    23881 gctttgatgg aaaaattccg taaagaagca ccgacagaat ttgccggagc aaaagttatt
    23941 caaacagaag actttaaaga attgacgaga acagaccgtg aaggaaacgt agaaaaacta
    24001 acaacaccgc catccgacgt attgaagtat acattggaag aaaatggctg gatcgctatc
    24061 cgcccttcag gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg aagctctaca
    24121 gaagacgcag ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat gacagaagaa
    24181 taaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt
    24241 tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt agagaaagtc agtcaattat
    24301 tcaaaatgtt gagtgatccc actcgtctta aaattctgct atatctgaaa gacggggaac
    24361 aaaatgtgac agcaatcacc caagcagtgg aaatggagca gtctgctgta tcgcatcaac
    24421 tacgtttatt aagagaaaat catgtcgtca agtctcatcg cgagggaaaa gcgattttgt
    24481 atagtcttga tgattctcat gtactggata ttttgaacca gacactcaaa catgtggaac
    24541 atcaataacg ataggtgaaa atagataaac actggagtgt gacaacgaag aaatttcgtt
    24601 gtcacactcc agtgtttttt aagaaaaaga caaaaatgat taatcgcttt tttctgttgt
    24661 ttcagttcca taataactat gatcttctag gtctaaagca ttcaaaataa gcgaagccgt
    24721 ttcttcaata gataaagatg ccacattgat cactacacat cctagttttt cataaagatc
    24781 attcgcaaaa tcgagttcag ctctaatttt ttcgatatcg gaataagcag tatcagggtt
    24841 taatccgtaa gagcgcatcc gttctttacg gatattattt aaaatatctg ggttattcgt
    24901 tagtcccacg atttttttag gatcgatttc ccacagctgt ttgggaatat gtgcttgggg
    24961 aatcaaaggt aaattagcaa cttttaaatt tttatttgct aaaaacaagc tcaaaggcgt
    25021 tttggaagtt cgggatacgc ctagcaaaac gacatctgct tctaaaaaac cacgaggatc
    25081 tttcccatca tcgtatttta cagcaaattc cattgctttg atccgtttga aatagttctc
    25141 attcaataaa tggagggcac caggttgccg aattggcttg ataccagtta aacgttcaat
    25201 ttctgatatc ggtggcgtca tcacatcgaa actaaataat tgatgttctt cacaaaaaga
    25261 acgtgttatc tcgatcagtt cttgattgat taatgtatgc aggacaagtg ctttttcttc
    25321 taatgcatct tgcaatgcct gccttaatgt tttttcatct gtcacgaacg ttctgcggaa
    25381 taggttgaac tcaacggtag gatactgagc catagtagct tgcgctagtt ttgtcgcagt
    25441 ttctccagca gagtcggaga tgacaaaaaa agtaacgatt tctgtagaat cggaagacat
    25501 ggataaaacc tcacttgcat tttttttatg tttattatac catactctag aatgcgtact
    25561 tcgtaatcat tttgatttgg aggaataagt catgaaaaaa cagacattga tcgaacaatc
    25621 attagaaaca ttgaaaaata acggttttaa atatactaaa aaaagagaag cattgttaac
    25681 ttatttagtg aagcgtaacc gctacgtatc tgctaaagaa gtttttgact atatgaatca
    25741 ggaattcaaa ggggtaagtt atgatacgat ttaccggaat ctccatgatt ttgcagagat
    25801 cgatttactg gaagaaaccg atttaaatgg tgaaatgaag tttcgttttc attgctgtca
    25861 gggggaaatc ggccatcacc atcattttat ctgtacagtg tgcggaacaa cgaaagaaat
    25921 ccatatgtgt ccaatgaatt atttcgaaga acagctaggt ggatgcacga tcgaagggca
    25981 tagattcgag attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat aaaataacat
    26041 ttgataattt aaaaaaaaag caagatttcc ttgacggtca aaacagcatt agatataata
    26101 tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt tactttttaa
    26161 gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga atcacttgac
    26221 gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca agaatctcgt
    26281 aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga agcagctaga
    26341 aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta
    26401 gattaaacga tgacatgaaa gcagcgatga aagcgaaaga taaggaatct ttacaagtca
    26461 tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac gacttgacag
    26521 aagaagaaga actaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat tctttgcatg
    26581 agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa attgtaatag
    26641 tcgaaaaata tatgccggaa caactttccg acgaagaaat tcgacaactt gttcaagaag
    26701 caatcactca aactggtgct tcatcaatga aagaatttgg caaagttatg ggtgcaatta
    26761 tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg aaagaactat
    26821 tacaatctta attttatcat ttaaactgcg gacagtgatt gtccgcagtt ttttgtgttc
    26881 gtaaaaaaag aactaaaaaa cagctagaag aaagaataag attctttctt ctagctgttt
    26941 ttttcctgtt taatatctct tatgttaaat tgtgtttgat ttggttattc ttctggtagt
    27001 tcatttgctt cttgtttata ccctgatagg cgataggcgt aataaaagag taagctgcca
    27061 gcgtaaccaa gtaacgttgg gaccatccag ccaagtccta atgcagcaaa aggaacgtga
    27121 tgatgataaa atcctagtaa gcttgctacg acagtttgct gactcactaa tgcaggcgaa
    27181 tttgccaaca tatccaatac ggctggaacg gcagaaaaga acatcgttac ttgatagaca
    27241 aatggactct tttggaagaa ttttgctgtt aaggaaagca agattaacgc aagtgacaat
    27301 ggatacaaaa gcatcaatac tggtaaagac cattggatga tattatccaa tccggcattc
    27361 gcaacgacaa aagagacaaa agtcgttaac cgcagccatg tcatataact gactttaggg
    27421 aacagtttat ggaaatcttg tgcaaaagaa acaacgagtc ccatagcggt agtgaagaca
    27481 cctaatgtga ccattacccc taagaagcta gcaccgaagt tacctaaata aaaattagtg
    27541 atctgagaaa gtgcagttcc gccattttct gataaaggca tcttattcaa actcatcgca
    27601 ccgagaagga ccagaccaaa ataaatcatg acttctaacg caacgctcag gctaccagct
    27661 tttgctgtta attctgtgac ttttttatct ttaaaaccaa gatcacgaac tgcatggaca
    27721 aaagtgacac ttaaagcaag taaagctact gcatcaaccg tgttgtatcc ttctaaaacg
    27781 ccatttgtca aagcgttcgt ctgataagaa tctgcagcag attggtttag attccccatg
    27841 ggttggaata aagaaacgac aaaaatgata gctaataatg caagaaacaa ggagtttaaa
    27901 tactttccga catatttgac caaacgtgtt ggatgtacag taaggaaata agcaagtaaa
    27961 aagaaaagag cagagaataa aaacatacct aatgaatcat atttttcagg caaaaagggt
    28021 ttagcagcca tttcaaaagc agttgctgct gtccgcggtg taccaaagaa tggtccgatc
    28081 gttaaatgaa caaggatcaa gaacaaggca gcataccatt ttccgacagg tttagctaag
    28141 tcatacagac catcactttt tgtcactact acagctaaga tagctaataa tgggaaaaga
    28201 gcgcctgaga tggcaaatcc tacaccagcg ctgatccagt ttgatccagc catttgacct
    28261 aagtggacag gaaagatcag attacctgac ccgaagaaca tcccgaagat aagtgaactg
    28321 accatcaata attgcttcca tgaataagtt tttgtttctt ccataaattt ttcctccaat
    28381 aattatcatt ttgagtaaca aaataaacgt tgcagaaact agaaatgcca ctaatatcag
    28441 attccatgaa aaccaccacc ttaaaatata aaaaaaagcc cttttccact tgggaaaagg
    28501 gctctagatt ctagaacggt accacctttt ttcgcttaaa aaagcttctt cattcacgga
    28561 cgcttttcga taaagttatc cgctagcctg ataatggagg cagccacaat gatctacgtt
    28621 ttcgacattg actcggaaat gatgttcgtc tattcgattt attgtcattt ctcacttaca
    28681 tgactcactg ggaataaatc gctgaatagc ctactgtttt cctcgacgct ttgctgtata
    28741 tttatttgtt gacgttataa tacacctgtt ttttttgaaa tgcaagaata tttcaaatat
    28801 tttgttttct ttttttgaaa caagaaaaat acccagagaa tacttgctaa gttagacgga
    28861 atacttgtcc tctatcttat gcatagctta catgatcagc tttttttatt caaacgagac
    28921 ctttagagaa tggaagcagc tattttttaa cgtttcttgg tacttatggt taagcaactc
    28981 ttttagaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa ataggaaaga
    29041 atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct ttagagatta
    29101 gattgacggc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag catataaaac
    29161 tgattgaaga aacaacagaa acaaccatcc atacacgtgg agaaatcatt cagatcattg
    29221 gcgaacaatc ccagatttcc ttggctgcct cagtcattcg gacattgcag gaattgatca
    29281 aacgaggaat ccatgtaagt acaccagatg tcgtgacggc actgaaaatg gggcaaaaag
    29341 gcacattaga ttattttgtt gaaatgtatg aggaagagat cgtcaaagac cgtaatggaa
    29401 aaccaatacg ggtaaaaaac agcggccaaa agaaatatgt cgaatcggta gccaaacatg
    29461 atatcgtatt tggcgtagga ccagccggga caggaaaaac ttttcttgca gtagtattag
    29521 caatcgctgc cttgaaaaaa ggtgaagtgc aaaaaatcat tttgacgcgg cctgctgtgg
    29581 aagcagggga aaatctaggc tttcttcccg gggatctaaa agaaaaagta gatccttatc
    29641 tgcgtcctgt ttatgatgct ttataccaaa tttttggatt agatcatacg aaccgattga
    29701 tggaacgagg cgtgatcgaa attgctcctt tagcttacat gcgggggcgg acattggatg
    29761 atgcattcgt tattttggat gaagcacaga acacgacagt tgctcagatg aaaatgttcc
    29821 tgacacgtct tggttatcaa tcaaaaatga ttgtcaatgg agatacgagt cagattgatc
    29881 tgcctcgagg gacaacaagt ggtcttgttc atgcagaacg aacattaaag caaatcaaga
    29941 aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta gttgccgaaa
    30001 tcatcaaagc ttatgaaaag gcagatctcc atcaagaata agctttgctt aggaggataa
    30061 gcacatgttg aataaaacac tgcagaaact acaagaaaaa cttggaaagg cagcattgcc
    30121 tttcctgctg ttcttgtttt ttatcattat ctgcggcgtt acatttagta gtgtcaaaca
    30181 aaaggccaat gatttcagag aaggccaagt agcagaagaa agtatccgtg cgaacaaaac
    30241 gatcgagaac acggaagaaa ccgaacaaaa aagaaaactg gcagcagaag cagtcactcc
    30301 agaatacact taccaaaaag atcttgcaga tgaccagaac aatcggatca agcaactatt
    30361 tgaattgatc gataaaacga atgatgccat caacaaatct tatgacgaaa agattgataa
    30421 ggcaaaggaa gatgaaaata tcccaaagcc tactacagaa gagcggatcg ccgcgttgaa
    30481 gaaaaatttt gaaaatgtga atgcagaaaa tgttgcgttt tatcaaaaac taccgaacaa
    30541 tttttattcg acgatatttg aaatgactga aactgagatc catactgtga gggatgagag
    30601 tctgaagctc attgacgaac agatgagcaa acaagttcgt gaaagtgaat tggaagcttt
    30661 taaacaagaa gcagaagagc aaatccaata tttgaatgtc acaccagcac aacaacaaat
    30721 gattcgctac ttagtggatc aaggtatcgt agtgaatgac gtattgaatg agaaaaagac
    30781 agaagaattg aaacaatccg cacgagaagc tgtacaacca gtgatgatct tccaaggaga
    30841 aatcattgtt cgtgaaggta atcaaatcga tgcttcagct atgaaaaaat tggaattact
    30901 aggactgact aatcaaacaa cttcgatttt tccgctaatt gccatggttt tagctgtttt
    30961 gcttcaaatc ggcgtactga tttataattc acttcaattc caaaatgcag gagaaagaac
    31021 aaaatacgtg cttttctatg tgacagctat gtcagtcagc atagtgctga tgaaattttt
    31081 ccaattattc caaacggaac aagctactta tatcccgctg ttttatcccg ctgcttttgc
    31141 accacttgtt ttaagctttt tcttgaaccg aagagcaggg atcatggctg gtattttcca
    31201 agcagtatct gcacttttca ttttttatga ttcgattggc acgaactttc taacgattat
    31261 cttgatgtct tatgttttct ctgggctcat ggcaacgatt gtcagacgca aacggatctc
    31321 agaacaagga ttatctgctg ccatgtgggt cattattttt cctgtgatga tggatgtcat
    31381 tttgatcgtc tatcaaggaa tgagctttgg tgatgcaacg acttggttaa tgcttgtctg
    31441 cgggttcact ggtgcactct tctcattctt gctgacgatc ggtctacacc catatattga
    31501 attgatggtc aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc caaatcatcc
    31561 tttattgaaa cagttgctgg aagaagcgcc aggtacttat catcatagta tgatggtagc
    31621 taatctaagt gctaacgctg tagcagaaat cggcggacgt tctttgctaa ctcgagtagc
    31681 ttgctattat catgatatcg gcaagatcaa acatgctagc tttttcgttg agaacttgcc
    31741 ttcaggagcg gaaaacccgc acaatttctt attgccagaa gacagtaaac atattatctt
    31801 tggacatgtg acagatggag ccaaaatatt ggaagattat aatatgccgc aaatggtgat
    31861 cgatatttgc aaacagcatc atggaaccac gctaatgcgt tatttctaca tcaaagcgaa
    31921 agaacgcaat ccggaagtaa cagaagagca attccgttat cctggaccaa aaccgcaaag
    31981 tcgtgaagca gggatcgtca atattgctga cagttgtgag gctgcagtac gggcaatgga
    32041 tcatccgacg agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag agcgtatttc
    32101 cgatggtcaa ttagacgata gtggactgac cttgaaagaa atccgaaaag tggaaaaatc
    32161 attgatcagc ggtttaagta gcaccttcca ttctcggatc aaatatccta aaatgaaaac
    32221 agaagttgag aagataaagg aagaacaaga agaatagaaa gaagcgatgt ataatggata
    32281 ttacctttat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa atcgatgact
    32341 tgctgcaatt tgcagcatct tatttaaagc tgccagagga aacagaaatg tctgttactt
    32401 tcatggacaa tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa gatgcaccga
    32461 cagacgtcat cagttttgct ctggaagaag aaggcgaaga cgaaatccca gtgatttttg
    32521 aagatggaga gaatccattg cctcgtgtac taggagacat catgatttct attgaacgtg
    32581 ctgctgaaca agcagaagct tacggccatt catatgatcg ggaacttggc tttttagcgg
    32641 tacatggatt cttgcatatc aacgggtatg accatatgac accagaagat gagaaagaaa
    32701 tgtttggttt acagaaagag atcttggatg cctatggact caaaagataa aaacgtagag
    32761 aaaaacaaac atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa aaccgttttt
    32821 gaggaagaac gcaatatgag gaaacatgtt gcgttcggta tccttgcttt atttatgggc
    32881 gtgattttcc agcttgatcg gatagagtgg ttatggctgc ttttagctgt ttttctcgtt
    32941 tggatagtag agatcatcaa tactgttttt gaaaatgtag tggacatgtt tacggatttt
    33001 cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt tttagtgacc
    33061 gcttgttttg ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata tcatttgttg
    33121 tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc gcaatcgtcg
    33181 gccgcccgaa tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa aaaattgcca
    33241 ttatgagtga caaggcacag acaactagaa ataaaatcca aggagtatat actacaccag
    33301 aagctcagct gatttttatc gatacaccag gaatccataa accaaagcat cgtctaggag
    33361 attttatggt agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc ttatttatga
    33421 tcagtgctga tcaaaaacgc ggccggggag acgattttat tattgaacgt ctaaaaaacg
    33481 tgcattctcc agtttatctg attatcaata aaatcgataa gatccatcca gacgagttgc
    33541 tgggaatcat cgaagattat tcgacgcaga tgccttttgc acaagtgatt ccgatctctg
    33601 ctacagaagg aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa atgccagagg
    33661 gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttt atcgtttctg
    33721 aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat tctgtcgcag
    33781 tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag gccacgatca
    33841 tcgtcgaaag agatagccaa aaaggaatca ttatcggcaa aggcggaaaa atgctgaagc
    33901 aagtaggaac gaaagcaaga aaggatatcg aaaatctttt aggagacaaa gtatttctag
    33961 aactttgggt caaagtccaa aaagactggc gagacaaaaa agtctatctg caagactttg
    34021 gctaccgaaa agacgaatac tgatattctg aacttcttat aggttaagta tagaaataaa
    34081 ctggataaaa atccgcacgt tgagaaaaac ggcggaagat cgaaacataa ataatctgga
    34141 tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag ccagcttgca
    34201 gaatcgaaag gcttgatttt atctagccgt aattacaaag aaaaagataa attagtcaaa
    34261 atctttacgg aatcttctgg caaaatgatg ttttatatca aaggaatcca tcggcaaaat
    34321 cagccattag ctccggcttt gctccccttt accgaagcag tctatatcgg taattttcga
    34381 agtgaaggtt tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg gaacatccaa
    34441 gaagatatct ttttgagcgg ttatggtacc tatttgctaa atctagtaga tgcagcaatc
    34501 gaagatcatc aatatgaccc gcatttattc caatttacgc aacaagctct gcagcggatg
    34561 gatcaaggag atgatgcaga aatcatcacg aatatctttg aagttcaaat actgcaacgc
    34621 ttcggaatcg cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa aggaaaattt
    34681 gactattctt ccaaatctgg cggtgtgata tgcgagaaac actggccaat ggacgaacac
    34741 cgttatcatg cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc tatttcttat
    34801 gataagatca gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg tgacctcatt
    34861 gaccagttgt atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt cattgatcaa
    34921 atgaagtctt gggcacacgt attaaagacg ccagagaaaa atatgcctga aaaagagata
    34981 cctgaaaaag aagaataaat gaaagataca acaaaaaaat caaaactatt tgacaaatac
    35041 agggacttcc gtctattatt aaccgtagat taaatagatc gttcactgag aggatctatt
    35101 atataaacaa gaaaactgca aaatgaatac gtacattgac caagatcagt aaaaaacacg
    35161 tggtttagcg agttcgggag agtggaagcc ggacccaaac gatttttgaa gaggcacttg
    35221 cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa ccgcgaaatt
    35281 attcgtccct atgtctgtaa tcagacatag ggactttttg tcgttttctt tcctgtattc
    35341 ttattgacag caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa aaattgaccg
    35401 tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggctgc atgctgatgc
    35461 aagcttatga cacagaaaaa ggagcaggta cgatgagccc atatacattc ttacgggcaa
    35521 tcggaccgga accatggaat gctgcttatg tagagccatc ccgccgacca gctgacgggc
    35581 gttatggaga aaacccaaac cgtttgtatc agcatcatca atttcaagtc gtaatgaaac
    35641 cttcacctga aaatatccaa gagctttatt tagaaagctt gcgtctttta ggaattgatc
    35701 cattagaaca cgatattcgt tttgttgaag acaactggga gaacccatcg atggggtgtg
    35761 ccggtgtggg ttgggaagta tggcttgatg ggatggagat cacacaattc acgtacttcc
    35821 agcaagtcgg aggactacct tgtaaaccag ttacttctga gatcacttat ggactagaac
    35881 gtcttgcttc atatattcaa gaagttgaaa gtgtctatga tctggaatgg gctgatggtg
    35941 tgaaatatgg cgagatcttc aaacaaccag aatatgaaca ttccaaatat tcttttgaag
    36001 taagtgacca agacttgcta ctaagcaact ttgaccgttt tgaaaaagaa gctaaacgct
    36061 gtatcgatga aaatcttgtt catccagcgt atgattatat tttgaaatgc agccatacat
    36121 tcaatttatt agatgctcgc ggtgcagtat ccgtaacaga acgagcaggt tatctttccc
    36181 gtatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc gaaaaattag
    36241 gattcccttt attgaataaa aacacagcaa atccagaaaa catgaaggag gctgaataag
    36301 atggcaaaag acttattact tgaaatcggg ctggaagaaa tgcctgctca tgttgtcaca
    36361 ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa ccaattgact
    36421 tatgatacga tcgaaacgtt ttctacaccg cgccgcttag caatcaaagt aacaaacatt
    36481 ccagaacgtc aagcagacag tgaagaagaa gtcaaaggac cagccaaaaa aattgctttg
    36541 gacgcagatg gaaactggac gaaagcagct gaaggatttg tccgcggaca aggtctgacg
    36601 acagatgcta tcacttttag agaattaaaa ggcgtggaat atgtctatgt aacgaaatac
    36661 actcacggca aagaagcgaa agaagtattg acaggtttag cagatgtcat taaaagcctc
    36721 actttcccag taacgatgca ttgggcagaa tacgattttg aatatatccg ccctattcac
    36781 tggatcgtgg cattgttaga tgacgaagta atcccgtttg agatcttaga tgtcaaaaca
    36841 ggcagagcct ctcgaggaca tcgtttctta ggcgaagatg tgacatttgc gcatgcaaaa
    36901 gaatatgaag aaaaattgaa tgaacaattc gttattgctt caccgcaaaa aagacaagaa
    36961 atgatcgtta cacaagcaaa tgaattagcc gaaaaaaatc actgggttat tgatctagac
    37021 gaagatttac tagaagaagt caacaattta gtcgaatatc caactgtctt ttctggcgac
    37081 ttcgaagaaa aatatctttc tgttcctgaa gaagtactag tcacttctat gaaagaacat
    37141 caacgttatt tcgaggtacg gaacgatcaa ggtatgctgc ttcctcattt tatttctgct
    37201 cgtaatggtg atcgggtaaa attggaaaat gtggttcgag gcaatcaaaa agtattgacg
    37261 gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc aatcgaccaa
    37321 tacgttgaaa aactaaaaac tgttactttc catgaaaaaa tcggtagtat ctatgagaaa
    37381 atgcagcggg tacaagtcat tgcccgtatc attggcgaac aagtaggact atctgaggaa
    37441 gaattgatgg atctgcaacg agctgctgaa atctataaat ttgatctagt aaccaacatg
    37501 gtaggggaat ttcctgaact acaagggatc atgggcgaaa aatatgctct gctgaaaggc
    37561 gagaaacctg ctgtagcaac agcgattcgt gagcattaca tgcctatatc cagtgaagga
    37621 gagctacctc agtctacagt aggttctgtt ttagcgattg cagataaatt agatagcatt
    37681 ttttctttct ttgcagttgg gatgattcca agcgggtcaa acgatcctta tgccttacgt
    37741 cgccaagcat acggtgtcgt tcggatcatc gaatcgaaaa aatgggcctt tcctttgtct
    37801 gttctgcaag aaacgattag tgatgttatc agtaaagata cggatcgttt cggcattggt
    37861 ttatcagaag ggcaacaaca ggtgatcgac tttatcaaag gccgtctgcg ccaattattg
    37921 acaacgaaga atattcgtca tgatgtcatc gaagctgttt tgaatgcaga acaaaaagat
    37981 ctgacaaaag tatttgcagc agcacaattg ttcaagcagc atcttgcaga tgaagacttc
    38041 aaaccatcga tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc ggagcttgaa
    38101 aaacaatcag aagtagaccc tgaactgttc gaaaatgaag cggagaaaga actgcacaaa
    38161 gcagtagagg caatgaaaaa aagctttgct gaccaaacga tcaacgaaaa ttatgatcag
    38221 cttgtaagcc ttcgcccact gatcgaaaat tattttgatc agacgatggt catggcagaa
    38281 gaaccagcag tacgtaagaa ccgattaacg caattaagtc aaattgctgc catggctttg
    38341 tcgctagcaa gtttagatca aatcatcaca aaataatttc taatatgtaa ataaaccaga
    38401 gaataggcac aaaa
//
LOCUS       DACDCV010000016        35562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDCV010000016 DACDCV010000000
VERSION     DACDCV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.016"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            242..2008
                     /locus_tag="IV041_000825"
     CDS             242..2008
                     /locus_tag="IV041_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8381639.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2110..3369
                     /locus_tag="IV041_000826"
     CDS             2110..3369
                     /locus_tag="IV041_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8381640.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3516..4817
                     /locus_tag="IV041_000827"
     CDS             3516..4817
                     /locus_tag="IV041_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381641.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4820..5671
                     /locus_tag="IV041_000828"
     CDS             4820..5671
                     /locus_tag="IV041_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8381642.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5684..6499
                     /locus_tag="IV041_000829"
     CDS             5684..6499
                     /locus_tag="IV041_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8381643.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6650..7816
                     /locus_tag="IV041_000830"
     CDS             6650..7816
                     /locus_tag="IV041_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8381644.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8046..8693
                     /locus_tag="IV041_000831"
     CDS             8046..8693
                     /locus_tag="IV041_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8381645.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8777..9721
                     /locus_tag="IV041_000832"
     CDS             8777..9721
                     /locus_tag="IV041_000832"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8381646.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9856..11307
                     /locus_tag="IV041_000833"
     CDS             9856..11307
                     /locus_tag="IV041_000833"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8381647.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11328..12164
                     /locus_tag="IV041_000834"
     CDS             11328..12164
                     /locus_tag="IV041_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8381648.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12164..12823
                     /locus_tag="IV041_000835"
     CDS             12164..12823
                     /locus_tag="IV041_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8381649.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12949..13638
                     /locus_tag="IV041_000836"
     CDS             12949..13638
                     /locus_tag="IV041_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381650.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13644..14600
                     /locus_tag="IV041_000837"
     CDS             13644..14600
                     /locus_tag="IV041_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8381651.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14644..15156
                     /locus_tag="IV041_000838"
     CDS             14644..15156
                     /locus_tag="IV041_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381652.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15412..15960)
                     /locus_tag="IV041_000839"
     CDS             complement(15412..15960)
                     /locus_tag="IV041_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8381653.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16049..17230)
                     /locus_tag="IV041_000840"
     CDS             complement(16049..17230)
                     /locus_tag="IV041_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8381654.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17466..18974)
                     /locus_tag="IV041_000841"
     CDS             complement(17466..18974)
                     /locus_tag="IV041_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8381655.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19133..19324
                     /locus_tag="IV041_000842"
     CDS             19133..19324
                     /locus_tag="IV041_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381656.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19527..20444
                     /locus_tag="IV041_000843"
     CDS             19527..20444
                     /locus_tag="IV041_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8381657.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20437..21510
                     /locus_tag="IV041_000844"
     CDS             20437..21510
                     /locus_tag="IV041_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381658.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21658..22176
                     /locus_tag="IV041_000845"
     CDS             21658..22176
                     /locus_tag="IV041_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381659.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22157..22549
                     /locus_tag="IV041_000846"
     CDS             22157..22549
                     /locus_tag="IV041_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8381660.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22625..23560
                     /locus_tag="IV041_000847"
     CDS             22625..23560
                     /locus_tag="IV041_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8381661.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23693..24472
                     /locus_tag="IV041_000848"
     CDS             23693..24472
                     /locus_tag="IV041_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8381662.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24465..24782
                     /locus_tag="IV041_000849"
     CDS             24465..24782
                     /locus_tag="IV041_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8381663.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25026..26636
                     /locus_tag="IV041_000850"
     CDS             25026..26636
                     /locus_tag="IV041_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8381664.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26793..27641
                     /locus_tag="IV041_000851"
     CDS             26793..27641
                     /locus_tag="IV041_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8381665.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27872..28351
                     /locus_tag="IV041_000852"
     CDS             27872..28351
                     /locus_tag="IV041_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381666.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28384..30612
                     /locus_tag="IV041_000853"
     CDS             28384..30612
                     /locus_tag="IV041_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8381667.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30741..32384
                     /locus_tag="IV041_000854"
     CDS             30741..32384
                     /locus_tag="IV041_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8381668.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32457..33002)
                     /locus_tag="IV041_000855"
     CDS             complement(32457..33002)
                     /locus_tag="IV041_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8381669.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33025..33510)
                     /locus_tag="IV041_000856"
     CDS             complement(33025..33510)
                     /locus_tag="IV041_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8381670.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33500..34312)
                     /locus_tag="IV041_000857"
     CDS             complement(33500..34312)
                     /locus_tag="IV041_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8381671.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34451..>35562)
                     /locus_tag="IV041_000858"
     CDS             complement(34451..>35562)
                     /locus_tag="IV041_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8381672.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11637 a         6397 c         7110 g        10418 t
ORIGIN      
        1 ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca
       61 ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc tatttggcaa
      121 gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt
      181 gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc
      241 aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa
      301 tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct
      361 atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa
      421 aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag
      481 tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga
      541 tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat
      601 accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg
      661 gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac
      721 tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag atttgcaagg
      781 tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct atttttgtcc
      841 catatttaaa gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc
      901 cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat
      961 acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga
     1021 cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc
     1081 agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac
     1141 ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata
     1201 tttgctggat atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga
     1261 tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat
     1321 caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg
     1381 ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag attattttgc
     1441 aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta
     1501 cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat
     1561 cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct
     1621 tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga
     1681 cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg
     1741 ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc
     1801 tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac ggaaactaga
     1861 gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga
     1921 aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg
     1981 acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt
     2041 gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa tacaaaggag
     2101 gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga
     2161 atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat
     2221 gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata
     2281 aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc
     2341 gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg
     2401 gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg
     2461 ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc
     2521 tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat
     2581 acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt
     2641 gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc
     2701 tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca
     2761 gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc
     2821 aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag
     2881 caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac
     2941 aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct
     3001 tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac
     3061 ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa
     3121 atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat
     3181 gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca
     3241 gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca
     3301 aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa
     3361 aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat
     3421 aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca
     3481 gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata
     3541 tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg
     3601 gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc
     3661 ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag
     3721 atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca atcattttct
     3781 tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg
     3841 gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc
     3901 cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc
     3961 tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt
     4021 taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta
     4081 atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc
     4141 aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac
     4201 gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga
     4261 gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca
     4321 taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga
     4381 cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg
     4441 ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag
     4501 caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc
     4561 caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta
     4621 acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct
     4681 ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt
     4741 tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt
     4801 tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact
     4861 ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca tctatccgct
     4921 tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct
     4981 ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac
     5041 ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt
     5101 aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat
     5161 gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat
     5221 ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg
     5281 tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt
     5341 agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt
     5401 accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg
     5461 ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg
     5521 cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc
     5581 aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac
     5641 cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt
     5701 aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc
     5761 atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac
     5821 tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc
     5881 gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt
     5941 acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc
     6001 tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat
     6061 ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca
     6121 accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc
     6181 cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt
     6241 catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca
     6301 actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat
     6361 aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag
     6421 tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa
     6481 agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat
     6541 gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt
     6601 tcttttttag tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa
     6661 aactttatac aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg
     6721 ggacggtaca acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat
     6781 ccatgaagaa atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc
     6841 ctatatggat catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta
     6901 tcagcaagca aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga
     6961 aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga
     7021 cttttataaa ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc
     7081 tgaagatacg ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt
     7141 tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac
     7201 tgctaccaaa gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga
     7261 tttgattacc aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat
     7321 ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga
     7381 acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc
     7441 aaaaggaaca gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg
     7501 gatcaataaa tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa
     7561 tattacaaaa aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt
     7621 gacattagaa cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat
     7681 gggtgattcc ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga
     7741 atcaagtaat atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc
     7801 aatgcatcgt gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa
     7861 gttttatgat gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct
     7921 tagtttcttt ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga
     7981 cagaaaattt tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg
     8041 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
     8101 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
     8161 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
     8221 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
     8281 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
     8341 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
     8401 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
     8461 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
     8521 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
     8581 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
     8641 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
     8701 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
     8761 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
     8821 tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg aactggctca
     8881 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
     8941 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
     9001 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     9061 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     9121 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     9181 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     9241 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     9301 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     9361 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     9421 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     9481 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     9541 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtaccga
     9601 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     9661 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     9721 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     9781 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     9841 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     9901 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     9961 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
    10021 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
    10081 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
    10141 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
    10201 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
    10261 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
    10321 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
    10381 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
    10441 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
    10501 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
    10561 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
    10621 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
    10681 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
    10741 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
    10801 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
    10861 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
    10921 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
    10981 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
    11041 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
    11101 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
    11161 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
    11221 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
    11281 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
    11341 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
    11401 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
    11461 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
    11521 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
    11581 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
    11641 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
    11701 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
    11761 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
    11821 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
    11881 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
    11941 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
    12001 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
    12061 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
    12121 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat
    12181 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
    12241 agggattggc ttcgtgtcgt ttcttttagg aaatctggta gggttgcttt tgacagtttt
    12301 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
    12361 aggcattccc gcactagtcc tgctgtttct gttatatttt gggttacctt atcaattaag
    12421 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
    12481 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
    12541 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
    12601 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
    12661 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
    12721 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
    12781 gcaacagtat ttagaaaaac gttctatgca tcagtcaatc tgaaaaatag aaatggtgct
    12841 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
    12901 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
    12961 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
    13021 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
    13081 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
    13141 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
    13201 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
    13261 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
    13321 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
    13381 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aacagatcaa
    13441 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
    13501 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
    13561 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
    13621 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
    13681 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
    13741 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
    13801 accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
    13861 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
    13921 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
    13981 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
    14041 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
    14101 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
    14161 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
    14221 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
    14281 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
    14341 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
    14401 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
    14461 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
    14521 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
    14581 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
    14641 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
    14701 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
    14761 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
    14821 gatcaaattg ctggaacagc tgcattagtc tcaagcgtgg atcccgttta cacaaaaata
    14881 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
    14941 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
    15001 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
    15061 aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac
    15121 ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat
    15181 aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag
    15241 tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat
    15301 tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca
    15361 tagcttttgt cacgacctct cggcttatcc tacgagtatt attcgataca tttatatcaa
    15421 taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa
    15481 aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag
    15541 tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc
    15601 tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca
    15661 aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc
    15721 tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc
    15781 cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc
    15841 cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc
    15901 ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat
    15961 tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct
    16021 tttaaaagta actttgcttt tttgtatatt ataccagcaa gccagtcaca atagcactta
    16081 aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc
    16141 ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg
    16201 caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac
    16261 catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac
    16321 taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga
    16381 tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca
    16441 tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac
    16501 catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga
    16561 tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc agaatataac
    16621 cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat
    16681 aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac
    16741 gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta
    16801 aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg attttactta
    16861 atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga
    16921 taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt
    16981 tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat
    17041 caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga
    17101 aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt
    17161 tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata
    17221 aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata
    17281 aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc
    17341 attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct
    17401 ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac
    17461 tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt
    17521 cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt
    17581 gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca
    17641 ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt
    17701 gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca
    17761 tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc
    17821 aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca
    17881 gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact
    17941 accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta
    18001 actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg
    18061 atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca
    18121 cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca
    18181 ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta
    18241 ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct
    18301 gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg
    18361 aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaaggaa
    18421 gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt
    18481 tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga
    18541 tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata
    18601 aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag
    18661 ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa
    18721 tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct
    18781 tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca
    18841 cgtttcgcat gcttgtcgtc aactagcttc ccaccaaatt ctaaatataa tttgtcatag
    18901 tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa
    18961 ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg
    19021 attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact
    19081 tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa
    19141 aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga
    19201 aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac
    19261 gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa
    19321 ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt
    19381 tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc
    19441 tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa
    19501 cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc
    19561 cagacgaact agaggagacg aatgcggatc aggatacagg acaagaatcg tcttcttttt
    19621 acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt
    19681 ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat
    19741 tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc
    19801 gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct
    19861 cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta
    19921 ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat
    19981 attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga
    20041 tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa
    20101 tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc
    20161 aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata
    20221 ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag
    20281 accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta
    20341 gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga
    20401 atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa
    20461 tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa
    20521 caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata
    20581 cacgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac
    20641 gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc
    20701 agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc
    20761 gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca
    20821 atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg
    20881 ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat
    20941 ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg
    21001 accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca
    21061 atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat
    21121 cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc
    21181 catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac gaagccactt
    21241 cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc
    21301 gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta
    21361 cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg
    21421 tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc
    21481 ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc
    21541 agataattct gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt
    21601 ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg
    21661 tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc
    21721 gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt
    21781 tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt
    21841 tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt
    21901 ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc
    21961 tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta
    22021 tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac
    22081 agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat
    22141 cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat
    22201 actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga
    22261 agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga
    22321 aaaagaagtg ttcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga
    22381 agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa
    22441 tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat
    22501 caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa
    22561 aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa
    22621 taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa
    22681 tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct
    22741 aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga
    22801 taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata
    22861 ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat
    22921 ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat
    22981 agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga
    23041 aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca
    23101 cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc
    23161 tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg
    23221 attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag
    23281 tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga
    23341 acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat
    23401 tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat
    23461 caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat
    23521 tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga
    23581 agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt
    23641 tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa
    23701 aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta
    23761 tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt
    23821 atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat
    23881 gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg
    23941 ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt
    24001 ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc
    24061 aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc
    24121 atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga
    24181 ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag
    24241 cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt
    24301 attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat
    24361 gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt
    24421 caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt
    24481 tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc
    24541 tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg
    24601 tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata
    24661 ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac
    24721 gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt
    24781 aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc
    24841 cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat
    24901 tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct
    24961 tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa
    25021 tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat
    25081 gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag
    25141 atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga
    25201 tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag
    25261 aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca
    25321 tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc
    25381 cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg
    25441 ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt
    25501 atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt
    25561 attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg
    25621 cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag
    25681 acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat
    25741 ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta
    25801 ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag
    25861 agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat
    25921 tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa
    25981 gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga
    26041 ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat
    26101 acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag
    26161 cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc
    26221 atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa
    26281 tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta
    26341 ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa
    26401 agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt
    26461 ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga
    26521 tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa
    26581 aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta
    26641 actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt
    26701 ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg
    26761 gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt
    26821 cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt
    26881 tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg
    26941 gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt
    27001 tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt
    27061 cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag
    27121 taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag
    27181 ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc
    27241 gaatccgaga taatacctac gcttatctga ttgtagatga tttgaacaat ttagtacgag
    27301 gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc
    27361 tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat
    27421 ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac
    27481 tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga
    27541 aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt
    27601 taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac
    27661 ataccaaaaa tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa
    27721 agaacgagat ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt
    27781 caatcctcta taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt
    27841 actaaaaata aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa
    27901 accagtaatc gttgtagaag actatgataa agttgatgga agaaatgcat taaatacgga
    27961 tgcaaaaggg ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc
    28021 aggcaaggta tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat
    28081 cacacgtatt ttagatttag cgatcttagc tgcacaagga agtttatact tccaagatgc
    28141 ttatcgttat gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca
    28201 agggaaccga atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat
    28261 tttgtattac gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt
    28321 aaaacgtctt ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga
    28381 agcatggaga atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg
    28441 atctggaaga aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa
    28501 atcaaaggga attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat
    28561 gccgggtcgg ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat
    28621 acaaaagtga cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct
    28681 gtagtagatt ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat
    28741 acacttgaag ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct
    28801 aaagaaaaat tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat
    28861 tacagctatt acccttctga aatcgttcga gcgattgaaa aaggatattt attagagatt
    28921 caagagccga ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa
    28981 ccaaatggga tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt
    29041 gtgatcacta caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat
    29101 cggatgcaac atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg
    29161 atggcgaaaa ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt
    29221 ttgttggatg aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat
    29281 ttttattggg caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa
    29341 gttttgtata aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct
    29401 gacagtggat tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct
    29461 caatctgagc atacaaaagg aagaaccatt agcgccgaag aagcaaatga acgaaacatc
    29521 actgaaaatg ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa
    29581 ccaaatgagg gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa
    29641 atgcaaaaag aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga
    29701 gatgattctc aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag
    29761 caactgaata aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc
    29821 atcgttcacc gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa
    29881 gtaatgccaa tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa
    29941 acagagacgt accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca
    30001 tatggtgatt tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg
    30061 gtagctgtac ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca
    30121 aaaaaagcag cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt
    30181 tacggagata cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa
    30241 tttgaagacg gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac
    30301 aatcgtgatg gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca
    30361 accaaactgt tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc
    30421 aaaaaagcaa aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta
    30481 tttgtctccg cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt
    30541 cgttttgtag atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc
    30601 cgctatctat gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg
    30661 tttctagtta ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac
    30721 cgttaaagga gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct
    30781 atcaaatcta tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg
    30841 ggatccgtga aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc
    30901 cgatctatca atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag
    30961 aagagagatt cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag
    31021 ggatcaaagt gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg
    31081 aagaatcgaa aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg
    31141 tagatggcaa agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt
    31201 ctcatgaaac tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt
    31261 gggaaagcga gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag
    31321 ggatagatgg atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa
    31381 aagccacaga gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga
    31441 ccgatttgaa acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg
    31501 tcactgcaga agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg
    31561 aatttgatca tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat
    31621 tgaaacttgc attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat
    31681 atatggaaaa ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt
    31741 tttgggctat gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat
    31801 ttatttatca aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag
    31861 ttgatgcagt agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag
    31921 aagaagcatt ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat
    31981 ggacttcgga acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa
    32041 ataccaaaac gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct
    32101 ataagaacat gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag
    32161 aatatcttca tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat
    32221 atcttattat ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag
    32281 atcagtcctg gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca
    32341 tactagcacc ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag
    32401 ttcattagtt ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa
    32461 taattcgctc taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct
    32521 tgtccaaggg ttttcaaacg aggtctgaca ggtacttgga catgtttgat atgcataccg
    32581 attgatgtgt caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga
    32641 tcattgaact ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca
    32701 acagagacaa tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg
    32761 ttcaaatgtt cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt
    32821 aacgtacgga tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca
    32881 acgacttcgc ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca
    32941 gccaatatgt cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc
    33001 attttctaaa accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact
    33061 tgcagttata ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat
    33121 agaagccact ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat
    33181 cataactaag cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa
    33241 agaaccagca acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg
    33301 atatcctgaa atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt
    33361 tggaccataa agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt
    33421 cgtagccggt acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat
    33481 agcatagatc gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag
    33541 accagacact tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa
    33601 attttttact ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta
    33661 ttgcagaaga aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag
    33721 aaaatacctc ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc
    33781 ttgtgccgcc tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat
    33841 ggatcatttc tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca
    33901 aattaggcgt aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca
    33961 attgattgat cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga
    34021 tattttgttt tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata
    34081 acccaatttt gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa
    34141 gcaattccaa agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag
    34201 aagtcaaggc gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga
    34261 ttcctccacc agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt
    34321 cctccatgaa taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt
    34381 tttatctcat ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt
    34441 ccttaaatag ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt
    34501 tcattgtagc tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag
    34561 gaagttcatg gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag
    34621 caataatcgg ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat
    34681 ctaacgaatc taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat
    34741 ccacttgccg atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc
    34801 tatcccacca atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca
    34861 aagcccgtcc tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc
    34921 caactgaatc tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg
    34981 catcacttgt tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac
    35041 tttggccata tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct
    35101 tcattgtctg gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag
    35161 cttctggatg ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta
    35221 gtgcaattgg ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta
    35281 aaggcatcat aggaaatccc atggacatca aagaagagat accaatattg aatctggaat
    35341 gcactggaga gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt
    35401 tttgccattt cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac
    35461 cagttgtacc agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag
    35521 aaacagacaa agcaggttgt tccattaaat aaagaatagg aa
//
LOCUS       DACDCV010000017        33120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDCV010000017 DACDCV010000000
VERSION     DACDCV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.017"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..859)
                     /locus_tag="IV041_000859"
     CDS             complement(107..859)
                     /locus_tag="IV041_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8381673.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(874..2121)
                     /locus_tag="IV041_000860"
     CDS             complement(874..2121)
                     /locus_tag="IV041_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8381674.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(2197..2343)
                     /locus_tag="IV041_000861"
     CDS             complement(2197..2343)
                     /locus_tag="IV041_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP8381675.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(2447..2758)
                     /locus_tag="IV041_000862"
     CDS             complement(2447..2758)
                     /locus_tag="IV041_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8381676.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2760..2957)
                     /locus_tag="IV041_000863"
     CDS             complement(2760..2957)
                     /locus_tag="IV041_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8381677.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(3351..5078)
                     /locus_tag="IV041_000864"
     CDS             complement(3351..5078)
                     /locus_tag="IV041_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8381678.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(5071..6264)
                     /locus_tag="IV041_000865"
     CDS             complement(5071..6264)
                     /locus_tag="IV041_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8381679.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(6451..7095)
                     /locus_tag="IV041_000866"
     CDS             complement(6451..7095)
                     /locus_tag="IV041_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8381680.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(7189..7323)
                     /locus_tag="IV041_000867"
     CDS             complement(7189..7323)
                     /locus_tag="IV041_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8381681.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(7325..9457)
                     /gene="feoB"
                     /locus_tag="IV041_000868"
     CDS             complement(7325..9457)
                     /gene="feoB"
                     /locus_tag="IV041_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8381682.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(9454..9927)
                     /locus_tag="IV041_000869"
     CDS             complement(9454..9927)
                     /locus_tag="IV041_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8381683.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            10328..10552
                     /gene="nrdH"
                     /locus_tag="IV041_000870"
     CDS             10328..10552
                     /gene="nrdH"
                     /locus_tag="IV041_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8381684.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            10711..12870
                     /gene="nrdE"
                     /locus_tag="IV041_000871"
     CDS             10711..12870
                     /gene="nrdE"
                     /locus_tag="IV041_000871"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8381685.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12920..13885
                     /gene="nrdF"
                     /locus_tag="IV041_000872"
     CDS             12920..13885
                     /gene="nrdF"
                     /locus_tag="IV041_000872"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8381686.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13974..15113)
                     /locus_tag="IV041_000873"
     CDS             complement(13974..15113)
                     /locus_tag="IV041_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8381687.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(15422..16135)
                     /locus_tag="IV041_000874"
     CDS             complement(15422..16135)
                     /locus_tag="IV041_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8381688.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(16389..17090)
                     /gene="nagB"
                     /locus_tag="IV041_000875"
     CDS             complement(16389..17090)
                     /gene="nagB"
                     /locus_tag="IV041_000875"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8381689.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            17324..18856
                     /locus_tag="IV041_000876"
     CDS             17324..18856
                     /locus_tag="IV041_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8381690.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(19047..20249)
                     /locus_tag="IV041_000877"
     CDS             complement(19047..20249)
                     /locus_tag="IV041_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8381691.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(20331..21020)
                     /locus_tag="IV041_000878"
     CDS             complement(20331..21020)
                     /locus_tag="IV041_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8381692.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(21126..21494)
                     /gene="rplL"
                     /locus_tag="IV041_000879"
     CDS             complement(21126..21494)
                     /gene="rplL"
                     /locus_tag="IV041_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8381693.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(21547..22050)
                     /locus_tag="IV041_000880"
     CDS             complement(21547..22050)
                     /locus_tag="IV041_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8381694.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(22460..23149)
                     /gene="rplA"
                     /locus_tag="IV041_000881"
     CDS             complement(22460..23149)
                     /gene="rplA"
                     /locus_tag="IV041_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8381695.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(23253..23675)
                     /gene="rplK"
                     /locus_tag="IV041_000882"
     CDS             complement(23253..23675)
                     /gene="rplK"
                     /locus_tag="IV041_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8381696.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            24018..24686
                     /gene="sdaAB"
                     /locus_tag="IV041_000883"
     CDS             24018..24686
                     /gene="sdaAB"
                     /locus_tag="IV041_000883"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8381697.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            24699..25571
                     /gene="sdaAA"
                     /locus_tag="IV041_000884"
     CDS             24699..25571
                     /gene="sdaAA"
                     /locus_tag="IV041_000884"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8381698.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(25662..26681)
                     /locus_tag="IV041_000885"
     CDS             complement(25662..26681)
                     /locus_tag="IV041_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8381699.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26854..27741)
                     /locus_tag="IV041_000886"
     CDS             complement(26854..27741)
                     /locus_tag="IV041_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8381700.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27818..28363)
                     /gene="nusG"
                     /locus_tag="IV041_000887"
     CDS             complement(27818..28363)
                     /gene="nusG"
                     /locus_tag="IV041_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8381701.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(28464..28634)
                     /gene="secE"
                     /locus_tag="IV041_000888"
     CDS             complement(28464..28634)
                     /gene="secE"
                     /locus_tag="IV041_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8381702.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(28658..28810)
                     /gene="rpmG"
                     /locus_tag="IV041_000889"
     CDS             complement(28658..28810)
                     /gene="rpmG"
                     /locus_tag="IV041_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8381703.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28972..29616
                     /locus_tag="IV041_000890"
     CDS             28972..29616
                     /locus_tag="IV041_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8381704.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(29672..30175)
                     /locus_tag="IV041_000891"
     CDS             complement(29672..30175)
                     /locus_tag="IV041_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8381705.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(30188..30234)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30772..31737
                     /locus_tag="IV041_000892"
     CDS             30772..31737
                     /locus_tag="IV041_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8381706.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31836..33008)
                     /locus_tag="IV041_000893"
     CDS             complement(31836..33008)
                     /locus_tag="IV041_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381707.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32995..>33120)
                     /locus_tag="IV041_000894"
     CDS             complement(32995..>33120)
                     /locus_tag="IV041_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381708.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        10215 a         6694 c         5700 g        10511 t
ORIGIN      
        1 taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc actagtgaag
       61 gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa acagtgcttt
      121 ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg gccaactctg
      181 aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag atatttacta
      241 aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca ctaattcttc
      301 cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt aattttgaag
      361 cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa
      421 caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct aataacaata
      481 actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca aatcctaacg
      541 tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt gtaaaaatga
      601 tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg ttaacttcat
      661 gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt ttgaggtatt
      721 ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta tcgtgaaaaa
      781 gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga tcttcacata
      841 atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa cttgagtagt
      901 aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt tattgatttc
      961 ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc cttttttcat
     1021 tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa tttctatctg
     1081 tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta tagatgcttc
     1141 aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg gaacaggaac
     1201 ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat ttgcttggta
     1261 tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta aataacgata
     1321 ttcaaactta ttggttagat aagaattaga atagatctct aattctttta tttctttttc
     1381 caatatcgaa tcatggtttt gattagcgat ttcttttaaa ctcagcaata aatttttata
     1441 gtcatgtcta aatctggcaa atttttcttg atctttttcc agtgaatcag tatatttttt
     1501 tagataaacc aattcttttc ttttcaattg ctgttcatat tgttctcgtt gcctaatagt
     1561 tattctaata aataaaaata gaacaacaaa catttgaatg attaaaaata ttaagacacc
     1621 aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa ataaaccaat
     1681 tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt caacaatcct
     1741 atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag aatcaagcat
     1801 tatctggaat actacataac catacccttt tatcccttca aatgaaaaca tatacgtcat
     1861 aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata gtaatttatt
     1921 taaaagcact atattagttt tcttcttttc ctttaatata aaataacttc cgattatagg
     1981 tatagctgcg gaaacatcag taaataattc aacaaaaaaa gtgagtggta tcaataaaca
     2041 tacaaaaaga tattttcttt tgttaagatc agtattagtc atataaaata agagaaaaaa
     2101 gaaaaagttt cccacgaaca ttcttaatag ataagctttt gtatcgatag taatcaagag
     2161 atcccctcct gattttagga tatcataaaa aaaattttag ttacactttt taaataatga
     2221 aaaaacacat tccactaaat ttgatgccgg ttttccttga ggttttgttc cagcacctcc
     2281 tttaattttt ttaagctctt gatctgaaca ttgtttagca tttagtctat ttttttcttc
     2341 cattacgatt ccccctacaa actataaata cttttatgtc actgattcaa tatacctatt
     2401 tttctaatca aaaaattttg agacatattt aatattataa gtatcattaa aattgagatt
     2461 tatctccata atctgctcga tataaaccat ttattccagc agttcttcca atttcactca
     2521 aatttcttat taattcctct tgatattcag tgaatcttat tttattagta taagcagtaa
     2581 aatatagata attcaccaat ctatttatta aaggaccggg atctaattca tatttttgtt
     2641 tttctaattt taaatatacc ttttgtaaaa cctcaagaat atcagaatat ttaggatctt
     2701 tacttataga tatatcatta tataattcat gaacaatttg cttagcattt tttttcattt
     2761 tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa
     2821 gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca
     2881 taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa
     2941 atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta
     3001 acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat
     3061 aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat
     3121 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
     3181 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttggacgttt cgaagatgag
     3241 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
     3301 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
     3361 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
     3421 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
     3481 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
     3541 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
     3601 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
     3661 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
     3721 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
     3781 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
     3841 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
     3901 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
     3961 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
     4021 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
     4081 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     4141 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     4201 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     4261 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     4321 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     4381 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     4441 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     4501 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     4561 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     4621 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     4681 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     4741 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     4801 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     4861 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     4921 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     4981 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     5041 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     5101 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     5161 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     5221 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     5281 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     5341 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     5401 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     5461 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     5521 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     5581 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     5641 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     5701 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     5761 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     5821 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     5881 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     5941 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     6001 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     6061 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     6121 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     6181 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     6241 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     6301 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     6361 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     6421 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     6481 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     6541 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     6601 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     6661 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     6721 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     6781 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     6841 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     6901 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     6961 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     7021 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     7081 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     7141 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     7201 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     7261 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     7321 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     7381 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     7441 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     7501 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     7561 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     7621 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     7681 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     7741 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     7801 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     7861 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     7921 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     7981 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     8041 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     8101 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     8161 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     8221 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     8281 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     8341 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     8401 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     8461 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     8521 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     8581 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     8641 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     8701 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     8761 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     8821 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     8881 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     8941 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     9001 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     9061 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     9121 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     9181 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     9241 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     9301 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     9361 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     9421 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     9481 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     9541 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     9601 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     9661 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     9721 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     9781 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     9841 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     9901 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     9961 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
    10021 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
    10081 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
    10141 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
    10201 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
    10261 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
    10321 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
    10381 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
    10441 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
    10501 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
    10561 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
    10621 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
    10681 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
    10741 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
    10801 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
    10861 ctatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
    10921 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
    10981 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
    11041 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
    11101 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
    11161 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
    11221 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
    11281 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
    11341 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
    11401 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
    11461 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
    11521 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
    11581 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
    11641 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
    11701 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
    11761 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
    11821 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
    11881 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacggatat ctcatgtaac
    11941 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
    12001 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
    12061 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
    12121 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
    12181 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
    12241 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
    12301 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
    12361 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
    12421 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
    12481 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
    12541 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
    12601 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca acacgtcgat
    12661 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
    12721 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
    12781 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
    12841 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
    12901 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
    12961 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
    13021 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
    13081 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    13141 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    13201 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    13261 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    13321 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    13381 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    13441 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    13501 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    13561 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    13621 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    13681 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    13741 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    13801 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    13861 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    13921 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    13981 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    14041 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    14101 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    14161 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    14221 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    14281 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    14341 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    14401 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    14461 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    14521 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    14581 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    14641 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    14701 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    14761 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    14821 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    14881 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    14941 aacatataat agataaaacc agcaacaagc aatggtaaaa aaactgccga aagcattttc
    15001 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    15061 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    15121 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    15181 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    15241 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    15301 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    15361 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    15421 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    15481 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    15541 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    15601 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    15661 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    15721 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    15781 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    15841 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    15901 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    15961 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    16021 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    16081 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    16141 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    16201 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    16261 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    16321 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    16381 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    16441 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    16501 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    16561 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    16621 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    16681 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    16741 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    16801 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    16861 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    16921 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    16981 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    17041 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    17101 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    17161 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    17221 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    17281 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    17341 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    17401 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    17461 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    17521 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    17581 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    17641 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    17701 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    17761 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    17821 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    17881 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    17941 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    18001 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    18061 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    18121 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    18181 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    18241 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    18301 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    18361 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    18421 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    18481 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    18541 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    18601 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    18661 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    18721 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    18781 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    18841 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    18901 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    18961 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    19021 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    19081 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    19141 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    19201 ataaagtaca taaacataaa tacagtatta tttcggttgc ttgctgcttc acctagccct
    19261 tgtactcgtg tttgattggc gacttgacca aattgattcc cgacgtctaa taaaataata
    19321 gctaaaagca aaatggccaa atggcttccg ccaaccaata aagcaataaa gcttattgtt
    19381 tgcataacaa gtcccatata cacgattttt ctttcagaat aagaatctgc cagtcgtccc
    19441 acgataggtg cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat
    19501 gttccccaat tgtaggcggg actgctgata taaaaaatca acgtagacca aaagatcgaa
    19561 aatgttccaa acatgaaaaa tccactgact gccgcttctc ttaatagacg ttgagaagca
    19621 actaaatgcg gcaaactttt aagtgaatca aggtatgaaa cagctgtact actttgctgg
    19681 acagaacgcg gcattttcca gtaaagcaga agtgtaagaa ttccgactgc acataaagca
    19741 aataaataga tcatccgcca agaaaaaaga ctggcaacaa taccagatac cgtccgagaa
    19801 agcaaaatcc ccacgagtaa accgctaagc aatgtaccca tgaccttccc tcttgctttt
    19861 ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga aagtaatcct
    19921 attaaaaaag aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc ggccaacaga
    19981 gagacggcag ataacgctgc cattcgaatg atcaattttg gacgatccac cacgtcccct
    20041 aaaggaacta aaaacagtaa ccctaacgca tagcctaatt gggtcaacat cgtcaatatc
    20101 ccgactgcac ttgtgctaac agataaacta gctgctactt ttgttccaat cggctggatg
    20161 taatacatat tggcaacaac aacgccacaa gtgattgata atagtaaaat aagcgaagtt
    20221 gttaaaggtt ttttttcatt actcttcatt gatttccccc atttcctatc ttagtttaca
    20281 acttatggaa aagattggaa atagaaaaat acaactattc gccacactta ttatttgtgc
    20341 ttttgttttc cttgtttttt cttgcgatct ttataaatga agacaactaa ccctactagc
    20401 acagtaacac ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa cagagcttct
    20461 ccaccacacg catacaacac agacgaaggg atgactccaa tcacgatcga caatatgatt
    20521 tgctgtagag gcaatttcat cgcatctgca gcaaaattga ccatagaaga tggaataacc
    20581 ggtatcatat agcctaacat tacacctatt tttggatgtt tcatttgtcg gattgcttgc
    20641 acccaatgat tcgtttcggt tttcttatct aaaaatttta agcgatgcat aaggaaaatt
    20701 gcagtgatgt taccaagagc attacccaaa atgttgatga tcgcacctgt cagtggtcca
    20761 aagctcaaac cagcaatgac tccaactact gaagtaggaa tacctggaac agcacacatc
    20821 accgctgtca atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg
    20881 agtagctctg ccttactcgc tctcggattt aaaaataatt ggatgtctgt ctgatattct
    20941 aaatacaacc gatagcccac taccagtatc agaatgattc ccaacgagat caatatcaat
    21001 cggattattt tctttttcat atttcctcct gaaaaaaatc tatattcatt aaataatagt
    21061 ttaacataaa aacaaaaaag caccgaaatc caacatagga tctcagtgct tttaaatagt
    21121 aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt caattcttca
    21181 gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac tacagcttta
    21241 gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt aactttttgg
    21301 tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc agcagcgcca
    21361 ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc ttcaatagct
    21421 ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac gatgttttca
    21481 atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta atgctttatg
    21541 cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag cgtaagccac
    21601 gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt cgcgatttgg
    21661 caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga tgataccgcc
    21721 tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg ctggtgcgac
    21781 tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt ccatgccttc
    21841 aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt tcatttctac
    21901 gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta atccgcggta
    21961 gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat caacgattgc
    22021 tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga attttgatgg
    22081 aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc aaccagtatt
    22141 attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga aaggaaactc
    22201 ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat agtgattcac
    22261 aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt caataaacgt
    22321 cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt taattattaa
    22381 cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca gttggatcaa
    22441 tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca ggtccaaatg
    22501 ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt ttagctttta
    22561 gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca aatgaaactt
    22621 ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact ttaccagctt
    22681 tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta gggtttggca
    22741 ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc atatcaggtg
    22801 ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt
    22861 cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa
    22921 ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca cggatttgtt
    22981 ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt
    23041 ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa actttacttg
    23101 aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt ttggttcctc
    23161 cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca atatagagcc
    23221 aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc catgcttcgt
    23281 gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag gtcttccatt
    23341 tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt atttttgttt
    23401 ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc agctggcggt
    23461 gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac cggaatgatc
    23521 aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat gatattgata
    23581 cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc tgcaggaatt
    23641 tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt aagtccgtga
    23701 tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg aaaaaatcca
    23761 gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat ttgttttttc
    23821 aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt attctaaaaa
    23881 aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag catggttcaa
    23941 cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt cctacataac
    24001 aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat attattggac
    24061 ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc gggaaaatca
    24121 tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat gaatcatttg
    24181 caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg ttagggatgg
    24241 aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc ggtatggaag
    24301 tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa
    24361 aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc aatatccaga
    24421 tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc ttgatcgttg
    24481 tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct gagaccaata
    24541 cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc attatgataa
    24601 ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag ctgccaaata
    24661 tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg attgaagaat
    24721 tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca gttgaaatgg
    24781 aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa
    24841 tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga attaccggtg
    24901 gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt ggagaaacaa
    24961 tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa atggggttga
    25021 tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc
    25081 gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc gctggcgctt
    25141 tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga tgtcaagcag
    25201 aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa aatgggactc
    25261 ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga ctgatttgcg
    25321 atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc
    25381 aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt ccgccggatg
    25441 aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc aaagaaacag
    25501 cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag attttaaata
    25561 agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc ttcataacaa
    25621 aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat caatttcgta
    25681 ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga caattctttg
    25741 ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa cccataaatc
    25801 actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa gacatgctct
    25861 cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac ttcggcaatt
    25921 tttggcaata cttcaaagga cgcaggacct ccatctagtt gacgtccacc atgattggat
    25981 acccagatac catctgcccc tgcagagata gcgattactg catcttctgg cgattgtatt
    26041 ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc atctggtgtc
    26101 aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc attaccactt
    26161 gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg gaattgattg
    26221 atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat gatagcttta
    26281 gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc tttactcata
    26341 tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta
    26401 tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt agctgtatca
    26461 gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat aatcggtgta
    26521 tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc gcgtaatatc
    26581 cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt ccactcatct
    26641 tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt tacacgacct
    26701 tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt atgataagtc
    26761 atagtagatc cttctttcat cttcttgatt acatataaat catactaaac ttacaaaaag
    26821 taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa atcgacttac
    26881 aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt cacctgtcac
    26941 tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg ctaatgcact
    27001 gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag caatcaagaa
    27061 aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat agcggttaga
    27121 aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat catcgacaaa
    27181 atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg
    27241 tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc ccactatgtt
    27301 gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga tccattctgc
    27361 ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg gattgtctgc
    27421 ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa
    27481 ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa aggaattttc
    27541 tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta tcactttact
    27601 ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg tcgtctggca
    27661 accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac gtttgcataa
    27721 atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag aaagacttga
    27781 aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta tctacttgat
    27841 cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact ttcaattttt
    27901 ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg ccttcgatga
    27961 ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc ataccgatag
    28021 aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt ccggctccgt
    28081 gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat gaagcgtctg
    28141 tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt tctttttctt
    28201 tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa ataaagtcgg
    28261 ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt tcatatcctg
    28321 aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc tcctcatttc
    28381 attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca ttatagcact
    28441 aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct ttggatggct
    28501 gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac tactaacgta
    28561 tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc ttttacgctt
    28621 tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct ttatgtaatg
    28681 tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg ctttttcctt
    28741 cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt gctgcttttt
    28801 tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca taacagtatc
    28861 acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg ataaaattat
    28921 gatatgatta atttgaaatt aagctaaaat gagacaattg gaggtatagc gatgctatta
    28981 aaaacacttg tatataagaa aaaagatctg acaaccgtta cagagactag tacattagaa
    29041 gaagcattaa aaattttaga aacttccgga taccgttgtg tgccgatctt agattcttca
    29101 ggtaatattt tccgtggaaa catttacaaa atgcatatct atcgtcataa agcaaatggc
    29161 ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt catttacctg
    29221 gatacttctt tcttcaaagt attctttacg atcaaagagc ttccttatat tgctgtcctt
    29281 gacagtgaaa atcattttta tggtattttg acacatagta cgttgcttaa tatgctctct
    29341 caatcttgga gcgtagaaca aggaagctat gtactgacaa ttgcttccac aggaaagcaa
    29401 ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt
    29461 actcttgata tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct accggctaac
    29521 actgaagcga aagtatgtga aacgatcgtc aaccacttag agaaaaagaa ttataaagtg
    29581 gtagaaattg aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag
    29641 ctaatctgct ttcttttttc gctttttata attagacacg cttttcaaaa tcaagcgcgc
    29701 gatctaaata atacatcaat ggtgctgaaa cagccatgac gataaactca cttaatgcaa
    29761 gcgtgccata ggttgtccaa aatgcttggc cacctgttgg aactaacata taagcaatca
    29821 gtcccatgct gatggtaaag aagatcgtgt tcaatactaa acgcatcttt acatttttca
    29881 ctttgttttg caataaagct gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa
    29941 ccacatccat gataccaaaa ccaaagaagg cattatagac gatgaccccg cctaaaactc
    30001 cccaaagcaa tttacggtta aagacaacta aatgattcaa actttctgaa atacgaaact
    30061 ggatcggccc tgaggataca ggcgcaacta aaatagttaa cgctagataa agagccatga
    30121 tgatgccatt tacaacgatt acggataacc gttgtctgct tttcgttttt tgcattttct
    30181 ctcctctttt ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa
    30241 caaacaataa ttataacaga cttttcattt atttcaatcc gtcttatctg cttttaaaaa
    30301 agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt gggatatggt ataaagtaag
    30361 tgtaaggggt cataagaatt cccagtaaag cagacgaagt aggatctgca agaacagaat
    30421 tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt tgagcaacct cttactagag
    30481 aacggtctag caagcagctg aagatctaag tggacatgcc gaaccttgga acttctcctt
    30541 acgtaagtaa tggatcatgc gcttgctagg cttttttgtg tttttcggtc ttcggacctt
    30601 tttcattttc ctagacttct gctataataa ccaagaatta attttgtaac agattttttt
    30661 gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct tgctaattat tggttaacca
    30721 gcaaacagga gtattaaaat atcttgtttt attgaatagc aggaggaaat catgaaattc
    30781 cattatcata tattgatcaa tccatcggca ggcagcggaa atggctataa agtagcagaa
    30841 agaattttac cggtattaaa aaataagcat attgactata cacttcatta tagtgaatac
    30901 aaaggacacg atgctgagat tgctgaaact ttggcaaaag aaacattgat tccttggata
    30961 gaagaagaac agaaaacaat cgatactttc ccccttttga tcatcgtagg aggtgacggc
    31021 accttacatc aggtacttga tactttctat cagatggaag tagaattccc agttgcttat
    31081 attccagcag gttctggaga tgattttgct cgaggggcag gccttccaaa aaatcccaaa
    31141 aaaggacttc agctgatttt agcagctcaa tcaccagaaa aagtccacat aatggcttat
    31201 gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata atttcggtat aggattagat
    31261 gcagctatcg tccatgcgac aaatcattct tccacaaaaa agcgattgaa caaatacaac
    31321 ttaggatcat tttcgtattt attctctatt ctccgtgctt tattcactca aaaaggattt
    31381 ccaattctgg tagatgcagg aggaaaacaa ttaagttttt caaacgcatt tttatgtaca
    31441 gcaacgaagc atcctttttt tggcggagga atcgcgattg ttccaactgc tgatgcgtct
    31501 aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc
    31561 attcttttac ttatacagaa aaaacaattg aaatcgaaat atttccatca ctacacaaca
    31621 aacagattac gtatcgtttc aacaacaccg caacatggtc aagaagatgg agaagaaatg
    31681 gaaaaacgac cttttgatat cacaataacc acaactaaac agttgttttg gtgttaataa
    31741 aagaggtttt gtcagaagct acatctggaa caccgtttat tcggaaaaaa ggactgtgac
    31801 acgacttttg tcacagtcct ttttttacta tgcagttaat tctttgccca attgggttgc
    31861 catcgatagt ttctttcttt acccaaaata aattggatca ttgaaaacac cgcagcaaaa
    31921 gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt
    31981 gtttcactga tcaagttatt attttgaaca aaccaatatg ttggtgtgaa tgccgctatt
    32041 ttgctaacca tttctggcaa gtattctgct ggtacaaaca ccccaccgat aaagcaactg
    32101 cctgtgataa aaatatcttt gatcccaccg ctggtttctg agttttttac caatgaagtg
    32161 atacaagccg aaaagctaat gatcggcaat aaaaacaaga aagtatttaa aatatgatac
    32221 cataccgtta tatcccacga ggtgtggatc actaacagaa ccattagaag taagaagcca
    32281 ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt
    32341 ttggtaatgg gcgcactctg gtttcgttta ctgatctctt tgcgattaaa tgctagattg
    32401 atgacgctga aaccgctaaa aatggataga aacaacccat aagacaagaa attaaaggtt
    32461 gtgcccgtca acagcttttt catccgctga ctatatcccg cacttagctc aacttctcca
    32521 gtaaccgaga gtgccatttg tgtctgttcc atagctgcat cgattgtttt atcttcttct
    32581 tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa
    32641 aaggcatctg ggcgactctg tatcttgatc gtcggcatct ttcctacttc gacttgttcg
    32701 gtaaagtctg caggcagatg taagataatg tttacacgat taaaataaag agcatcatcg
    32761 atttcttttt gtgaggttcc atccaatgta acgactgttt ccttgtcatt caaatattta
    32821 cgaaggccat cgactacagg cgactcttct tcactaatca aggcgatttt cgcatcttgc
    32881 agagaggtat tttcaacatc aatattttgc gcaaaaaaga atgtggcaac aacagtgata
    32941 attacaccta tcagtaaatt cgatttattt gctttaagca atgtccaaat cgttttataa
    33001 agcgtcataa cgcacccctc tttccatcca agcatttcct aacgcaaaaa ggaaaataaa
    33061 tccgatcaac catagaagat tggtataaaa actagccaaa tcagtgtaga aataaagttt
//
LOCUS       DACDCV010000018        32546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDCV010000018 DACDCV010000000
VERSION     DACDCV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.018"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IV041_000895"
     CDS             162..392
                     /locus_tag="IV041_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8381709.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IV041_000896"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IV041_000896"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8381710.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IV041_000897"
     CDS             1803..1979
                     /locus_tag="IV041_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8381711.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IV041_000898"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IV041_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8381712.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IV041_000899"
     CDS             2474..2941
                     /locus_tag="IV041_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8381713.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IV041_000900"
     CDS             3026..3910
                     /locus_tag="IV041_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8381714.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IV041_000901"
     CDS             4027..4260
                     /locus_tag="IV041_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8381715.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IV041_000902"
     tRNA            4348..4428
                     /locus_tag="IV041_000902"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IV041_000903"
     tRNA            4435..4506
                     /locus_tag="IV041_000903"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IV041_000904"
     CDS             5021..5728
                     /locus_tag="IV041_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8381716.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IV041_000905"
     CDS             complement(5788..6432)
                     /locus_tag="IV041_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8381717.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IV041_000906"
     CDS             6559..6846
                     /locus_tag="IV041_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381718.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IV041_000907"
     CDS             6954..7274
                     /locus_tag="IV041_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8381719.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IV041_000908"
     CDS             7355..8668
                     /locus_tag="IV041_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8381720.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IV041_000909"
     CDS             8665..9384
                     /locus_tag="IV041_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8381721.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IV041_000910"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IV041_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8381722.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IV041_000911"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IV041_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8381723.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IV041_000912"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IV041_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8381724.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IV041_000913"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IV041_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8381725.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IV041_000914"
     CDS             14821..15714
                     /locus_tag="IV041_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381726.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IV041_000915"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IV041_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8381727.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IV041_000916"
     CDS             17651..18508
                     /locus_tag="IV041_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8381728.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IV041_000917"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IV041_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8381729.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IV041_000918"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IV041_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8381730.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IV041_000919"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IV041_000919"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8381731.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IV041_000920"
     CDS             21264..22022
                     /locus_tag="IV041_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8381732.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IV041_000921"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IV041_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8381733.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IV041_000922"
     CDS             22950..23981
                     /locus_tag="IV041_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8381734.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IV041_000923"
     CDS             23978..24745
                     /locus_tag="IV041_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8381735.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IV041_000924"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IV041_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8381736.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IV041_000925"
     CDS             26713..27030
                     /locus_tag="IV041_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8381737.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IV041_000926"
     CDS             27030..27380
                     /locus_tag="IV041_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8381738.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IV041_000927"
     CDS             27547..28485
                     /locus_tag="IV041_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8381739.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IV041_000928"
     CDS             28500..29330
                     /locus_tag="IV041_000928"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8381740.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IV041_000929"
     CDS             29374..30399
                     /locus_tag="IV041_000929"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8381741.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IV041_000930"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IV041_000930"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8381742.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IV041_000931"
     CDS             31477..31917
                     /locus_tag="IV041_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8381743.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IV041_000932"
     CDS             31920..32522
                     /locus_tag="IV041_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8381744.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10802 a         5526 c         7051 g         9167 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaa
//
LOCUS       DACDCV010000019        32178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDCV010000019 DACDCV010000000
VERSION     DACDCV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.019"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IV041_000933"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IV041_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IV041_000934"
     CDS             complement(407..766)
                     /locus_tag="IV041_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8381745.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IV041_000935"
     CDS             complement(796..1137)
                     /locus_tag="IV041_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381746.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IV041_000936"
     CDS             complement(1134..1808)
                     /locus_tag="IV041_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8381747.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV041_000937"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV041_000937"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8381748.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IV041_000938"
     CDS             complement(4322..5512)
                     /locus_tag="IV041_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8381749.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IV041_000939"
     CDS             complement(5533..6060)
                     /locus_tag="IV041_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8381750.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IV041_000940"
     CDS             complement(6107..8896)
                     /locus_tag="IV041_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8381751.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IV041_000941"
     CDS             complement(9043..9243)
                     /locus_tag="IV041_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8381752.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IV041_000942"
     CDS             complement(9696..10016)
                     /locus_tag="IV041_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381753.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IV041_000943"
     CDS             complement(10519..11637)
                     /locus_tag="IV041_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381754.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IV041_000944"
     CDS             12108..13415
                     /locus_tag="IV041_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8381755.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IV041_000945"
     CDS             13536..14735
                     /locus_tag="IV041_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8381756.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IV041_000946"
     CDS             complement(14762..16030)
                     /locus_tag="IV041_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8381757.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IV041_000947"
     CDS             complement(16200..16841)
                     /locus_tag="IV041_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8381758.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IV041_000948"
     CDS             complement(17250..17753)
                     /locus_tag="IV041_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8381759.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IV041_000949"
     CDS             complement(17876..18640)
                     /locus_tag="IV041_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8381760.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IV041_000950"
     CDS             18748..19023
                     /locus_tag="IV041_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8381761.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IV041_000951"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IV041_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8381762.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IV041_000952"
     CDS             complement(20579..20980)
                     /locus_tag="IV041_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8381763.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IV041_000953"
     CDS             complement(20994..21803)
                     /locus_tag="IV041_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8381764.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IV041_000954"
     CDS             complement(21790..22680)
                     /locus_tag="IV041_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8381765.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IV041_000955"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="IV041_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="IV041_000956"
     CDS             complement(23348..24289)
                     /locus_tag="IV041_000956"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP8381766.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV041_000957"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV041_000957"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8381767.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IV041_000958"
     CDS             complement(25274..26470)
                     /locus_tag="IV041_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8381768.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IV041_000959"
     CDS             complement(26488..27216)
                     /locus_tag="IV041_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8381769.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IV041_000960"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IV041_000960"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8381770.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="IV041_000961"
     CDS             complement(28628..29086)
                     /locus_tag="IV041_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8381771.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="IV041_000962"
     CDS             complement(29083..30546)
                     /locus_tag="IV041_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381772.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IV041_000963"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IV041_000963"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8381773.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="IV041_000964"
     CDS             31602..>32178
                     /locus_tag="IV041_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8381774.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DACDCV010000020        31819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDCV010000020 DACDCV010000000
VERSION     DACDCV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.020"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            318..1928
                     /locus_tag="IV041_000965"
     CDS             318..1928
                     /locus_tag="IV041_000965"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8381775.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2220..3089
                     /locus_tag="IV041_000966"
     CDS             2220..3089
                     /locus_tag="IV041_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8381776.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3254..4525
                     /locus_tag="IV041_000967"
     CDS             3254..4525
                     /locus_tag="IV041_000967"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8381777.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4522..5817
                     /locus_tag="IV041_000968"
     CDS             4522..5817
                     /locus_tag="IV041_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8381778.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5916..6179
                     /locus_tag="IV041_000969"
     CDS             5916..6179
                     /locus_tag="IV041_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8381779.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6624..7073
                     /locus_tag="IV041_000970"
     CDS             6624..7073
                     /locus_tag="IV041_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8381780.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7130..8548
                     /locus_tag="IV041_000971"
     CDS             7130..8548
                     /locus_tag="IV041_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381781.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8548..9408
                     /locus_tag="IV041_000972"
     CDS             8548..9408
                     /locus_tag="IV041_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8381782.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9582..10355
                     /locus_tag="IV041_000973"
                     /pseudo
     CDS             9582..10355
                     /locus_tag="IV041_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            10502..10867
                     /locus_tag="IV041_000974"
     CDS             10502..10867
                     /locus_tag="IV041_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8381783.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11015..12223)
                     /locus_tag="IV041_000975"
     CDS             complement(11015..12223)
                     /locus_tag="IV041_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8381784.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12242..12520)
                     /locus_tag="IV041_000976"
     CDS             complement(12242..12520)
                     /locus_tag="IV041_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381785.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12713..13369
                     /locus_tag="IV041_000977"
     CDS             12713..13369
                     /locus_tag="IV041_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8381786.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13811..14608)
                     /locus_tag="IV041_000978"
     CDS             complement(13811..14608)
                     /locus_tag="IV041_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8381787.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14605..15789)
                     /locus_tag="IV041_000979"
     CDS             complement(14605..15789)
                     /locus_tag="IV041_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8381788.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15814..17061)
                     /locus_tag="IV041_000980"
     CDS             complement(15814..17061)
                     /locus_tag="IV041_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8381789.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17276..19021
                     /gene="dnaX"
                     /locus_tag="IV041_000981"
     CDS             17276..19021
                     /gene="dnaX"
                     /locus_tag="IV041_000981"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8381790.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19041..19355
                     /locus_tag="IV041_000982"
     CDS             19041..19355
                     /locus_tag="IV041_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8381791.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19380..19976
                     /gene="recR"
                     /locus_tag="IV041_000983"
     CDS             19380..19976
                     /gene="recR"
                     /locus_tag="IV041_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8381792.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20040..20684
                     /locus_tag="IV041_000984"
     CDS             20040..20684
                     /locus_tag="IV041_000984"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8381793.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20699..21028
                     /locus_tag="IV041_000985"
     CDS             20699..21028
                     /locus_tag="IV041_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381794.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21042..21980
                     /gene="holB"
                     /locus_tag="IV041_000986"
     CDS             21042..21980
                     /gene="holB"
                     /locus_tag="IV041_000986"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8381795.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22016..22840
                     /locus_tag="IV041_000987"
     CDS             22016..22840
                     /locus_tag="IV041_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8381796.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22833..23180
                     /locus_tag="IV041_000988"
     CDS             22833..23180
                     /locus_tag="IV041_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8381797.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23249..24121
                     /gene="rsmI"
                     /locus_tag="IV041_000989"
     CDS             23249..24121
                     /gene="rsmI"
                     /locus_tag="IV041_000989"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8381798.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24230..25351
                     /gene="dinB"
                     /locus_tag="IV041_000990"
     CDS             24230..25351
                     /gene="dinB"
                     /locus_tag="IV041_000990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8381799.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25405..26007)
                     /gene="nrdG"
                     /locus_tag="IV041_000991"
     CDS             complement(25405..26007)
                     /gene="nrdG"
                     /locus_tag="IV041_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8381800.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26322..28511)
                     /gene="nrdD"
                     /locus_tag="IV041_000992"
     CDS             complement(26322..28511)
                     /gene="nrdD"
                     /locus_tag="IV041_000992"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8381801.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28837..29604
                     /locus_tag="IV041_000993"
     CDS             28837..29604
                     /locus_tag="IV041_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381802.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29594..31636
                     /locus_tag="IV041_000994"
     CDS             29594..31636
                     /locus_tag="IV041_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381803.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        10233 a         5676 c         6663 g         9247 t
ORIGIN      
        1 tttctgattt gcttaggttt aacgttagct atgtttatat caagcttatt atgtgggtta
       61 aatttttttt gcactcataa aggacttaag aaataatttc tcaaaggata tcgaaaaaga
      121 aaaaattgta agcggatagt attcatttga gtacttgact ttcccttagt cagcttgtat
      181 tatacacctt gggcaccctt ttagagggtc agaagtttac agaaatttcg gctccctatt
      241 cttattgaag aatagggagc tttgtttatt gtctgagact tttcctgcac ataaattaaa
      301 aaggagtttt tgaacttatg acaaaatata tttttgtgac cggcggtgtc gtttcatcaa
      361 tcggaaaagg gattgttgca gcttctttag gacgcttatt gaaaaaccgc ggcttaaaag
      421 taactatcca aaaatttgat ccatacatca acgtggatcc ggggacgatg agcccatatc
      481 agcacggaga agtattcgta acagatgatg gtgctgaaac agatttggat ctgggccact
      541 acgaacgttt tattgacatc aatttgaaca aatactccaa cgtgacgaca ggaaaaatct
      601 attctgaagt tttgcgtaaa gaacgtaagg gcgaatactt gggagcaact gttcaagtta
      661 ttcctcatat tacaaatgag atcaaagaaa aaatcatgcg agcagctaaa atgactgatt
      721 cagatgtgat cattacagaa gtagggggaa cagtagggga tatagaatct ttgccattcc
      781 ttgaagcact tcgtcaaatg aaagcagatg tcggtacaga taatgttatg tatatccata
      841 caacattgat tccttacttg aaagctgctg gagaaatgaa aactaaacca acgcagcaca
      901 gtgtaaaaga gctacgtggt ttaggtatcc aaccgaatat tttggtggta cgtacagagc
      961 aacctgtttc tcaaagcgtg aaaaacaaat tggcacaatt ttgtgatgtc gaaccagaag
     1021 cagttatcga gtcgccagat gtagatacac tttactccat cccattagtt ttacaagcac
     1081 aaggaatgga tcaaattgtt tgcgaccatt taaaactaga tacaccagaa gcagacatga
     1141 cagaatggaa agaattagaa gaacgtgtct tgaacttgaa gaaaaaagta cggattgcgc
     1201 ttgtcggtaa atatgtcgag ttaccagatg catatatctc agtagttgaa gcattgaaac
     1261 attctggttt cgcctataat tcagacatcg agatcgattg gatcaaagca caagaattaa
     1321 cgagagaaaa tgtagaagaa cgtttgaaag acgcagatgg aatcttggtt cctggtggat
     1381 ttggtgatcg cggagtagaa gggaaaatcg aagcgattcg ttatgctcgc gaaaatgacg
     1441 ttccgttttt aggtatttgt cttggtatgc agatggcttg tgtcgaattt gcacgtaacg
     1501 tcgttggatt agaagatgcg tcatcagctg aaacgaatcc agatacagca aacaatatta
     1561 ttgatttaat ggctgatcag gaaaacatcg agaatttggg tggaacttta cgccttggat
     1621 tatacccatg taaaatcaaa aaaggaacga agacggctga agcatatgat ggagccgatg
     1681 ttgtgcaaga acgtcaccgt caccgctatg agttcaataa caaatatcgt caattgtttg
     1741 aagaacaagg gctagtcttt tcaggtgtct caccagataa ccgtttagta gaaatcgtag
     1801 agctgccaga gaaaaaattc tttgtgggat gccaattcca cccagaattg atttctcgtc
     1861 caaatcgccc gcaaaaatta atcaaagcat ttgtcggagc atcattagca gatcacgaaa
     1921 caaaataatt tttgaacttg atagaaagag aaaagtagca agagaagggg ataagcaatt
     1981 ttcttgtcac ttttctcttt ttctgatttg ttattatttg taggaaaaaa gatagaaagc
     2041 aaaagacttt tttcgattaa agcgctattg gatattttat tttttgcaaa aaacttcttg
     2101 aaaattttca ttttcattga ttttagtaga aaactaccga aatgtttggt aaaatagggt
     2161 cgttggttaa gaattgtctt aacaaattta gttttacaaa acttaggagg aattttatta
     2221 tgccagtagt atcaggagct gaatttttaa aagctgctcg taaaggcggt tacgctgtcg
     2281 gcggattcaa cacaaacaac ttggaatgga ctcaagcaat ccttgaagcg gctgaagcaa
     2341 aacaagcacc agtacttatc caaacatcaa tgggtgcagc taaatacatg ggtggttaca
     2401 aaatctgtaa agatttaatc actgatttag ttgaagcaat gaacatcact gttccagttg
     2461 ctatccactt agaccatggt gattacgaag ctgctttgga atgtatcgaa ttaggctata
     2521 cttcagtaat gttcgatggt tcacatctac catttgaaga aaacttgaaa ttagctaaag
     2581 acgttgttga aaaagcacat gcaaaaggta tctcagttga atgtgaagtt ggttcaatcg
     2641 gcggagaaga agacggaatc atcggtaacg gcgaattagc tgatattgaa gaatgcaaac
     2701 aaatggttgc tacaggtatc gactacttag cttgcggtat cggtaacatc catggtcaat
     2761 atccagaaaa ctggaaagga ttagcttttg accacttaca agcaattgct gatgctgtag
     2821 gtccagacat gcctttagta ttacatggcg gatcaggtat tcctcaagat caaatcgaaa
     2881 aagctatctc aatgggtatc tcaaaagtaa atgttaacac tgaattccaa ttatcatttg
     2941 ctgcagctac tcgtaaatat atcgaagctg gtaaagataa agaaggtaaa ggattcgacc
     3001 ctcgtaaatt attagctcca ggtaaagcag caatcgttga agatgctaaa tctcatatcg
     3061 actggttcgg ttcagctaac aaagcgtaag catccacata acagatacga tatataggaa
     3121 tggagaacga cttttgaagt cgctctccat tcttttttac ttaaaacggc agaccatgct
     3181 cgtttctttt ttttatctat gataaaatac aagcatatcc atgaaaaaag attaataaac
     3241 aaggtggaaa cagatgaaaa aaatcgtaat caaaggaaac cgacctttag ctggtgaagt
     3301 aacgatcagc ggtgcaaaaa atagtgcagt cgcattgatt ccagctgcaa tcttagctga
     3361 ttccccagtg atcttggaag gtgtgccgga catacaagat gttcattcat taattgaaat
     3421 tttagagatc atgggcgcaa ctgtccattt ttccaacaac atactagaaa tcgatcctcg
     3481 agaagtcgta tcgattccga tgccgcacgg aaaaatcaat agtttgcgtg cttcttatta
     3541 ttttatggga acactgcttg gtaaatttgg cgaagcggta gtaggcctgc caggtggctg
     3601 ttatttaggc cctagaccga ttgacctaca cgtcaaagga tttgaagctc taggtgctga
     3661 agtgacaaat gaacatggtg ccatgtattt acggacagaa aataaaggat tgcgtggcaa
     3721 tcggattttc atggacgtgg tttcagtcgg agcaacgatc aatgtgatgt tagctgctgt
     3781 aaaagcaaaa ggacagacag taattgaaaa cgctgcacga gaaccggaaa tcatagatgt
     3841 ggcaacttta ctgaataata tgggggcaaa agttcgagga gcgggtacag atgtgatccg
     3901 tatcgaagga gtcgaaacat tacatggctg tcggcatttc atgattcctg accgcatcga
     3961 agcaggaacc tatctagcat tagcagctgc agtaggaaat ggtatcaaag tgaaaaatgt
     4021 gatctttgaa catttggaaa gctttatagc aaaattgcag gaaattggtg tccatatgaa
     4081 aatcagcgaa gatgaaatcg aagtttaccc ctcgaaagaa ttaaaacccg caaacatcat
     4141 gacctatcct tatccaggat ttgccacaga tctgcagcag ccacttactg cgttactatt
     4201 gatgactaca ggcacttcag aaatcatcga tacgatctat gcgaaacgtg tgaaccatgt
     4261 gccggaacta gcacgaatgg gcgctgatat taccgtggaa ggaaatatga tcatcgtgaa
     4321 tggtccaaat aaacttcacg gcacagaagt cgtggcttct gatcttcggg caggtgcttg
     4381 tttagtgatt gctggactat tggcagaagg gacaacgacg atttacaatg tagattatat
     4441 cttacgcggt tatgatcata ttatcgagaa actaacagcc ttaggggcat caattgaaat
     4501 gatagaggaa gtgggagctg aatgagcgat tatttgacaa tggcagaact agagaacaaa
     4561 acgttaaaag aagtttatag ttatgccaaa gaatttaaaa ttccttatta cagtaagatg
     4621 aataaaaagg aattatctct agctgtgatt cgtgcacaag cagagaaaca aggattttat
     4681 tatatggaag gaatattgga tatcgttggt caagatggtt acggtttttt acggccaatc
     4741 aattatggac caagtgcaga agatatctat atttctgcct cacaaatcag acgattcagt
     4801 ttgagaaatg gggacaaagt ggcaggaaaa gcccgtccac ctaaagaatc ggaacgttat
     4861 tatggtttga tgcacgtgga aagcgttaat ggaaaagatc cggaagaagc aaaagaacgt
     4921 ccgcattttc cagccttaac acctctttat ccgcaaagac agcttcaact ggaaacaaca
     4981 ccgcaaagac tatctacccg aatgatcgat atcttttctc caatcggttt tggtcagcgt
     5041 ggattgatcg tcgcaccgcc aaaagcaggg aaaacttctg tattaaaaga aatcgcaaat
     5101 ggtatcaccg aaaactatcc ggaagtagaa ttaatcgttc tactgattga tgaacgaccg
     5161 gaagaagtaa cggatctaga acgaagcgtg aaaggcgacg tggtatcttc tacatttgat
     5221 ctgcagccgc aaaaccatac tcgagtttct gaattagtat tggaacgcgc aatgcgttta
     5281 gtcgaagata aacgagatgt cgttatcttg atggatagta ttactagact tgctcgggcc
     5341 tataacttag tcgttccacc aagcggacgt acattgagtg gcgggattga cccagccgca
     5401 ttgtataaac ctaagaaatt tttcggggca gcaagaaaca tcgaagaagg tggcagtttg
     5461 acgattttag cgaccgcttt agtcgataca ggaagccgta tggatgacgt gatctatgaa
     5521 gaattcaaag gaactggaaa tctagaatta caactttcac gtgaattatc tgaacgtcgg
     5581 atcttcccag ccatcgatat caaaaaatca ggtacccgaa aagaagaatt attgatgcca
     5641 agtgaagaat tagaagaaat ttggaaactt cgaaaaaata tgactggaga ttcattagaa
     5701 tatacggaac aactgatccg ctttttgcgt aaaacaaaaa acaatcagca atttttcaaa
     5761 gaatttcaag atgtctcctt tggtaaaaaa aatccgacca gaaatctgaa aagataattg
     5821 caaccttgac tgaaacatgt taagatgtta atgttgtaaa tcgacaaatc aactctggtt
     5881 cagcaaatgg agcagggcaa aggagcgaaa aaatcatgaa agaaaacatc catccagatt
     5941 accacccagt agtattcatg gattcaacaa ctggtttcaa attcttgtca ggttcaacaa
     6001 aaacttcaca agaaacagtt gaatgggaag acggtaatac atacccagtc atccgtgtcg
     6061 aagtaacttc tgactcacat ccattctaca ctggacgtca aaaattcaca caagcagacg
     6121 gacgtgtgga ccgtttcaac aaaaaatacg gtctcaaaga cgcaaacgct gcggaataat
     6181 aaaaacgctg ataactcaac gtttagcaga ctatctgata gggatagtct gtttttttat
     6241 gttttttgcc aatttaaaac ttcgtttgat gctgaaaaag tcgtaggatc ataatgcaca
     6301 aaacaatgaa aaactagtat atagttttaa ttatccactt gataaaaaca ccttatatta
     6361 acgaaatgaa gaggggttat ttcgaagtaa aaaaccattt acctaaaatt agaagctaag
     6421 ctttcctttc aaggaaggct ttttgttttg ttatagactt gcgattgttt gtgagctaga
     6481 tcgttcattt taatgactta tacacgcgga tgatgggtct ttttcctctg ttcattcata
     6541 atattgaacg gtacagctag cgatgctata ctgacctcag ttccaagaaa acgttaacat
     6601 tttttgtggg ggagaggttt tttatgaggt ttgatcaatt gatcgcagaa gaactgattg
     6661 ttttcgatcc atcgatagat tcaaaaggat cattatttga gcgaataggg gatgtgttga
     6721 aaaaaacaaa taaggtaaga aatgttcaaa aagtcgttaa ggcactattc aaacgagagg
     6781 aggagatctc cacaggaata gaagatggat ttgggattcc ccatgcaaaa agcaaatatg
     6841 tgacaaaacc agcactgtgt ttttttcata cagccaaatt aacggactat agcgggatag
     6901 atgaaatgcc aattgaatat atttttgcca tcgtagttcc tgaaaaatca gcagatatgc
     6961 atctagagat tctcagcaca ctctccagac gattgatgaa tgcagatttt agaaagggaa
     7021 taaaagaagc cactacgcca gcagatatca tcagacagat tagtcaagaa tgaaaatagt
     7081 agagtctttg tccgagtatc gatagtgaag aaaaaggagg aaaataatca tgaaaatcgt
     7141 tggtgtaact gcttgcccga ctggaattgc tcatacttat atgtcggcag aaaaattaga
     7201 agtaacagca agaaatctaa accatcaagc aaaattcgaa acacaaggtg taaaaacaga
     7261 aaatcagtta tctgaacaag aaatcaaaga ggcagatgca atcattcttg cagtagataa
     7321 agaaatcgaa ttggaccgat ttgccgggaa aaaagtaaag cgtgtctcta caagccgagc
     7381 aatcaaagaa ccgcaagtag taatagacga agcattgaga gatattggcg tattcgttgt
     7441 gtctaatgaa aaagaaccag ctgcaaatga gaaaaagccg acaatctata atcatttcat
     7501 gaatggtgtg aattacatgc ttccatttgt gatcgcaggc ggaatcatca tcgcactgag
     7561 ttttgccttt gggattacag cggctgatcc caactcagca gactataatg tgttagcagc
     7621 tgcattttca aggatcggag gagatacagc gtttgccatg atggttcctg cgttaggcgc
     7681 ggggattgct acatcgattg ctggaaaagc aggttttgcc ccaggaattg ttgccggatt
     7741 actggcctct acaggtggtt caggttttct tggcggaatg attggtggcg tgctagcagg
     7801 gtatatctgt gattttctgg caaataaaat tagcgtaaaa aaagaatttt ctgctattta
     7861 tcagctgatt gtcgtcccct ttttatcgat cttaattatt gggcttttga tggtatttgt
     7921 gattgaacaa ccgatcacat gggtgttgga tgctttgaca aattggctga atagcttggg
     7981 aaatacatcc gggctattgt tcggattact tatcgggatc atgatggcag ctgatatggg
     8041 tgggccgatc aataaatcga cttcgacttt ctcgatcggt ttgatgtcag ctggagtaaa
     8101 tgcaccgatt gctgcttgta tggctgcagg aatgacacct cccctaggta ttgctttagc
     8161 cactttatta ttcaaacata aatttaccaa agaagaacgg acttcaggtc aatcatgctg
     8221 gattttaggc gcttcgtata ttacagaagg tgctataccg tttgcggtat ctgacccgat
     8281 ccgcgtgata cctagtttga tgacaggttc agcagtcgct gcagggattt ccatggcagt
     8341 aggagttact tcaatggcac ctcatggagg gatttgggtg atgctaattc caaatgttat
     8401 gaacaattta cctatgtatg tcttggcaat tgcatcaggt acagtcacta cagcctttat
     8461 gcttggacta ttgaaaaaaa cagtgaaaga gaaaaaacaa gacaagtgga cgaaaaatga
     8521 aaatctaaca aatgaggtgg aagcgtaatg ttatatacaa tgaaagagtt attgaaagtg
     8581 gctaaagaga atcaatttgc agtaccagcc ttcaatattt gcagctatga tatgatgaaa
     8641 gcaatcatcg aagaagcaga gagattagaa gcaccagtaa tcttagaaat tcatcctgat
     8701 gaaatcgcct atttaagtga tgagtttgtt gcggcagtaa gagcctatgc ttatacaagt
     8761 aaactgccga tcgttatcca cttggatcat ggcggtacga tagaagatgt cttacgggcg
     8821 attcgaaacg gctattcttc cgtgatgatc gatgcgtctt tgaaatcatt cgacgacaat
     8881 gtacagatta cgaaaaaagt ggttgcgtta gctcatgcgg taaatgtctc agtggaggct
     8941 gagcttggaa cgattgggaa taatggttct gctgaaggag gtgctgaaac aatcatttat
     9001 acagatcctg atcaagcgaa gatttttgtg aaagaaacag gaatcgatac acttgctgta
     9061 gcaattggaa cagcgcatgg cttgtatccg aaaaacagaa ctccaaaatt aaatatcgac
     9121 ttactgaaag aattgaacca acaaatcgat cttccatttg ttttacatgg cggttcaggt
     9181 aatccggacg cagaagtcag cgaagcagta aaatacggag ttgccaaagt gaatttgagc
     9241 tcagatctta aaagcgtctt tttcaatgct ttgagagaaa tattgagtga aaatccatcg
     9301 atgtacgagc cgaatgcaat ttatccagca gctaataaaa aggtacaaga agtggtcaaa
     9361 cataaaatgg aaattttgaa cacagtaggc aaggctagtt tatactaaaa ataaaaatgt
     9421 gtggtcaaga gggtatgaca atagtcttgt cataccctct taaccacaca cgatgttcag
     9481 aagtagtttt ttcgaaaaat ttcttcttat ttctcgaagc caatcacttc tgtcacaacc
     9541 tcttttttcc tgttactata taaagtaaat ggggtgattt tatgttgaat gaagtggaga
     9601 atagacagca ggagattgtg aattatctaa aaaacaacca atttgcaaga gtcaccgatt
     9661 tgatcgagtt agtaaattat agcgaagcca cgattaaacg tgaccttgtt tttctggaaa
     9721 aaagtggatt gatccgtaga gttcgcggtg gcgccatgct tattgacaat cgaaaaattg
     9781 acgtgcctta cctgatgaag atcaccaaat tagaagaaga aacagaaaaa cgtaagattg
     9841 cagatacagc ttcttctttg attcgagatg atatggtctt attcttggat tccagtacaa
     9901 cctctttaca cttagtcaga aacctagcaa gatttgatgg tttgcagatc attacgaatg
     9961 gcatcgtcac agctgctatg ttgagtgaat atacaacggc aaaagtatct gtattgggag
    10021 gggcaattgt tccgaaacga gcaacaatca acggttcaaa agcctataac gatgcattgt
    10081 catattatgc ggatttagcg ataatgagtt gtcgcgggct agatttcagc caaggagtaa
    10141 cagaaactca tgaaggagag gcaatggtca aaaaagcttt tagaaaacaa tcagaccggc
    10201 ttgccgtttt agttacacag caaaaaattg cacataaatt catgcatcaa gccattgctt
    10261 ttcatgaaat cgattaactg gtgacaagta aagaactgac aaaagaacag tatgcgaaag
    10321 taaaggaaca ccatatcgaa tgtctgtacg gataaattat tacgtgaatg ttctaagtgg
    10381 cttcctcatt tttagatagg accttatctt tgctcgcttc tctcagccga tggtataata
    10441 atgaaaaggt tatcttgtta gataatctta cttttttatc caggagaaag gagtgcagaa
    10501 aatgaaacat gtgaaggcgt tggtagtgaa ggcaatcatg atctgggcga ttctgtgggt
    10561 cgtgctgaca ggtttatacg gtgttagctt tatggattca acgattgttg gggtaattat
    10621 tgtagtcatg atctatgtgc taggggatct gttgatctta cgtaaagtag ggaacatcgc
    10681 agcgacgatc gcagatgctg gttcagctgc agttatcttg tggctctatc tatattttat
    10741 gaatgataca gtcgatattt ggatgaaagt attgattcct gctctattga tcggtgtggc
    10801 agaatggttc ttccacaagt ggttgctaag tcaaggcatc gtaccagatg aacggaaaat
    10861 ggaataaatc aacagttaaa ctattcttat cgataaatga aagctttaaa aataaataga
    10921 tgtttaatta agaataatca taaactattt ttactcaaaa aaccgctcag attattttca
    10981 gatctgaacg gtttttttta tctattcatc aagatcaaat ttcatttttc aatgattcaa
    11041 tcaattcgcc ctctgcttca tgactatgtt ttcggccatt aacaagatag agtgctgtac
    11101 atatcaatgg aatgactccg gcaatcacat acaatcctct aaatgaagta agagatttta
    11161 gcatccccat caaataagga ccaacgccta agcctaggtc taaaccaata aagtaggtag
    11221 ataaagcaat actgattcga tgttcgcgga cgatttttaa acaaacagcc tgtccatttg
    11281 acataaacgt accataacct aggccgacta atgctccaga gataagcaat gtcacactat
    11341 tcgtcgttac actcagtaaa aataatcctg ctgttaagaa taaatagcta ggatacatca
    11401 cgtattgttc tcctttgaca tcgaaaatcc ttccagtcaa tggacgtgta aatgtgatga
    11461 ccaaagcata tacgacgaag aaaaatgacc cagcagcaac taagtggatt acttttgtat
    11521 aagaagataa aaatgataaa acacttgaat aagaaagacc catcaaaaag gcaattcctg
    11581 taatgaaaag tgctttcttt tcaataaaac tattgatatt ccatgagcga agagatattt
    11641 tttgttcttc agataatgga atatttttta caggaaagac aaggcaggca atcgtaacaa
    11701 agaagaccag tacaattgaa aaccagataa tgaaacgaaa atcagtcata ttcagcaaaa
    11761 tcattcctaa aaatggccca acagcggcag ctaaactagt acttaatcca taataattga
    11821 tgccttcccc ttttttagat gcagggatat atgcagtaac gatagcattt gtagctgtag
    11881 aaactgtacc gtagccgaat ccattcaaga atcgaactaa atacaatgcc ccgatagttg
    11941 gcgtataaag atatgctaat gttgaaagga gatagaagat cgcgccgcct cttaaggtaa
    12001 acttccgacc aaatagttct aactgtttcc ccatgaataa gcgtgccagc aatgttccga
    12061 tgatatagat cccagaagca aaacctgctt ggctcaagct ggcgtgtaag gtgtcttgcg
    12121 caatgactgc catgatgacc atcaacagat aataaatcag gtagacaata aaattgatca
    12181 atgtgattcc aataaaatat ttattaaata atttcttttc catgtatgtt atacattccc
    12241 cttaagctga atatgctttt aatttttgta tttgaacttc tacatcgtga taattggatg
    12301 gatagcgatc aacaagttgg cgaataaata aatctcttgt agacataaac agatttaaaa
    12361 attcttcgca ttcgtttgtt ggattctcat ccactgcttt gatcaattcg atcgagtttt
    12421 gatacgcatc atttaaaaag actaaagaag ctttcatatc aaatcttgtt tgatatgctg
    12481 tttctaatcg attaatgatt gtgttcatcc agtaattcat gttttttccc tctattctta
    12541 aaaaatttta cttctgcagt ataacgaata aaaaaaagga aaaaaatcgc ataagtgatg
    12601 gaaaaaggta cttaacaaat gttaatatca ttagtaaaat acctttggac tttaaaaagt
    12661 tttaaagtac tgtatgatct ttttagatga aagtttggat gggggagcat cattggatag
    12721 tagagtctta aaggaatatt tggataaaaa tgattttccg attatacgca aaggatatca
    12781 taaatatttg acattcgaag ggatggaaga ttcccatacg tatatcttga aagagggaac
    12841 ggtaaaagca agtgttattt caaatgatgg gagagaattt aaccttcgct atatcaaaga
    12901 tcttgaaatc gtctctcttt taaaagacga atattcacaa ttcatcgatt cgccgtataa
    12961 tattcgagtt gaatcagaat atgcagagtt ttatcgaatc aatcgtgtgc gcttttggca
    13021 aaatatcaac caagacccaa agctacaaca ctatgtaaaa gagtattacc gctatcgtct
    13081 gctttattcg atgaaaaaaa tgcagcaaat gctattaaac ggaaagctgg gagctgtttg
    13141 tacgcaactt tacgaattat atgatttatt cggtgtgcgg acagaagaag ggatgctgat
    13201 cgatttttcg atcacgaatg aagaaatcgc caaattttgt ggcatctcaa ctgctagcag
    13261 tgtaaacaga attttaaaac aactgaaaaa agatggtatt atcaccgtta aagaccatca
    13321 tatcgttatt ttgaatatgg acttgttaga agacaatatt atcttttgat gaacaaagag
    13381 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    13441 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttaccaaga
    13501 ggttgtgagc ctgccgttta actctgagtc acaaggaaca atttcaaaaa acagcttttc
    13561 atgtttgatg aatattgtga cttgtgaccg aggagttggt aggagaacac cgtttaccaa
    13621 gaggttgtga aaaagctgtt ctgcatcaag taataagaag agccaagtaa aaacagtctc
    13681 ttatgttttt aacttggctg gacttatcac cgtagatgta agcttttgaa cgccgtttat
    13741 ttgcaacaaa aggggctgtg acacgatttt tgtcacagcc ctttttttgc gaacattcat
    13801 ttagcaattt ttagaaactt ctgccgccac cgccaaaagt cccgccaccg ctagaatggg
    13861 tagtacttcc gccaccgccg cttccaggag gtgggctttt ggggattctt cttgtcgtga
    13921 caaaagaatt tgtcagacga tcggtttttt cagttaattt gatactggat ttttctcgga
    13981 atggatattt gtagatgcct gatttcaatt gataacgcga aacagtcaca aaataaaaga
    14041 caagactaag gataagagcc aaaacaacag caatgatgat ttctactgtc gtcaaaacct
    14101 tatagcgtgt aattttgcca gtttcttcgt ctacacgata atgtcctcct ggaactccgt
    14161 cttccacata tgttgctgat ttagacaggt aagcttttgc agcattgtaa taggcagcat
    14221 ttgtcatctg atcataaaca tcatctaaga tcgaatcgat tctgctatca gtcaaataat
    14281 cgatcatatt accagaagtc gaaatgtaga tttcacgctg tcccatatct aatagcaata
    14341 ctgagccatt ttgatcattt ccgactgcgt ctcttaagta attatcagca aagtctcttg
    14401 gttcttctga attagatgta gtcgttacga tcaagactcg tcctttgatt ttttcattca
    14461 gcttttctgc ctgttgttcc aaagattgga tttgatctgt tgtgaataac ccagcctcat
    14521 catttaccgt agctgtatct gcatgagtag ccaccggcca gccaacactt actagcaaaa
    14581 gaaagaataa aaaaaacttt ttcatcatat caagtaacct ccgatcagaa agaggatcgc
    14641 tagcccagcg aaaatcccaa gagcggtcag tcccaatttt ttctggctga tcggcaggac
    14701 accgctgacc tttcctgttt gtccattcat tgcgtagtag tagacttttt tacttgagtc
    14761 attgcttcga taagtaacaa gccataccgg cagtaatacg taattattta cttcgtttgt
    14821 gattgaagca gaagttcgca caccggtcaa tgttgtatat ccatttgcgg tatcttttaa
    14881 cagcttctct gaatagtctt ttaattcaga ttgaatctgt gatttgattg cctcatattc
    14941 gatatctctt ttttctgctt ggaacccggc taaatactgg cttttaaaat caattgcttt
    15001 atctaacggg aaaggttgca cgccttcgac catcttttgt tgcgtatttt ttgataaggc
    15061 attttttact agttctttaa aagaaagctt gcctgctctt gaaacagcaa attgcttcgt
    15121 ttctgtgtat tcgatgtcac ctatccgcca tatgcggata actgtaccat tcgcctgcat
    15181 ggcaccatct acttcagcat ctgtagccca ataagggaaa tacacacctg tcaacttatc
    15241 gatttgatcc ttattgaaaa aagctttggg aacaaaccat tttttcttgg tccaagcgag
    15301 aaatctgttg attgcttctt ctttctcgac agcaaaagga agtactttat ttggcaagaa
    15361 ttctccgctt aaacgtcccg atagtacgac gggattatgg cagtagtaac aataagtcgc
    15421 agcagttgtc gcatcagtca cgatctctgc accacagttg ggacaaagaa aaagatctat
    15481 cgctgcggta ttatctgcag attcgtccgc tgtatctgaa aaaccgcctg cctcgtcaac
    15541 cgctttgcgc tcggcttcat tcatatcggc aagctgttct tgggctgtaa aagtaaaatc
    15601 ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc atacgcgctt ctttttgctt
    15661 ttgctcatat tggctgactt cttcttctgt atagatattc aggcagtatt cgcaatgaaa
    15721 tttttggtct ttgggatcaa aagtcaacgg accgccgcaa ttaggacatt tatgtgtaag
    15781 aacatccatc agctgcacac ctttctaaat aaattaatta acggggattc ctttaaacgg
    15841 tttaccacag tttggacaga atttagggat gccgttggaa agatccacga tctcattgca
    15901 ttcactgcat cgcatttgta gcttaggctg aacgttttct acaggtttgg cagctccgca
    15961 gttggaacag aattttccgg aattttctgt cccgcaaact ggacatgtcc aagtatccgc
    16021 atttccttgt gcagtttgtt gattttgttt ttctgcttgt tgtttgattt gttcttggtt
    16081 attttgagct gcttgagaaa gataagaacc atttgcattc attcccatac ctacacccat
    16141 aaagccggtc attgctcctg cttcattttt tccggctgct tccataccac gtgcaatgga
    16201 accttgcacg tatccttcac gtacgctagg atcacctagc atcgcccctt cgttacgcat
    16261 attgatcagt ttcacagaat catcggtgta agaaatgctg gctacagcca cagaaacaat
    16321 ctccatccca cgtaattcac gccaagaatc atccaatgca gtatccatgt atttgcttaa
    16381 ttccaaactc ttagatggta cataagaaat ccgttgacca tctgcagaca tctgattgat
    16441 tgctgattgt agcgctgtta ggaattcagc taaatactgc tcattgatat cattgatttc
    16501 cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat aaaatcgggt caacgacatg
    16561 aatagaataa gtgccatgtg cccgtaagaa taattctgcg ttatagaaat tgtcaaagta
    16621 gttcaacggc gcgcttgtac cgaatttgat tccctttatt tcttggagat tgatataaaa
    16681 aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat cggtcaaaag tttcagcaat
    16741 tgcttctttc agtgttccat tgaacatgga aggagctgcg tcatttttaa tggtgtaata
    16801 accttcctca gcggtataat cgatgatttt tccaccatcg accaaaagca tcatcatgtt
    16861 tggatagaca tggacgactg ttccgtctgt taaaacatct tctgttcctt tacgattgga
    16921 gccacgtttg tcatctttgc ggactttgac accttttgtc atgaccgttg tatcactcat
    16981 attgtctggt tcgatgactt ctagccattg atctgccaga ccgccgccaa ccatacttgt
    17041 tgctgctttg ataagaccca taaaaaattc ctcctctgat tcagctagtg ccaaacaatt
    17101 attgtttatt tattatacca taggaagatg tgaaacttct cggactagag acggaattga
    17161 aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg agaaggatag tagttttttt
    17221 acctaatatc acgtataata agagatagtg aattttttaa aaaaggggga tccttatggc
    17281 atatcaagca ctttatcgag tctggcgttc gcagcgtttc gaagacatcg ttggacaaaa
    17341 agctgtgaca cagacactaa aaaatgctat tgtttcccac aaaacctcac atgcctactt
    17401 gtttacagga cccagaggaa ccggaaagac gagtgcagca aagattttcg caaaagcaat
    17461 caactgtccg aacagtaaag acggtgagcc atgcaacgaa tgcgaaatgt gtcgatcaat
    17521 tactgctggt acgcaagaag atgtgatcga aatcgatgct gccagcaata acggagtcga
    17581 agaaatccga tttatccgtg atcgggcaaa ttatgcgcct acaaaggcag cgtacaaagt
    17641 gtacattatt gatgaggtac atatgctttc aacaggagct ttcaatgcct tgttgaaaac
    17701 attggaagag ccaaaagaaa gtgtcatatt catcttagca acgaccgaac ctcacaaaat
    17761 accagctacg atcatttcac ggactcagcg atttgatttt aaacggatca atacggcaga
    17821 tattgtcgaa catcttgaat ttatcttgaa agaatcgact atcagctatg aaccagctgc
    17881 attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg gatgcattga gtattcttga
    17941 tcaggcaatc tcgtttagtg aaggaacaat tactttagat gatgccatgc aagtgacagg
    18001 tagcttaact gacgaaatga tggacagtta cttgagtgct tgcgtgaaaa aagaagtacc
    18061 agaagcattg gaaacattga accaaattct cgcagccggc aaagaatcac gtcgctttct
    18121 agaggatatg ttacaatact gccgtgactt gctgatgtat cagcaagcac ctaacctagt
    18181 tgaggaacaa tcgagtacga tcagaagtac atttacagaa ttggcacaaa ctgtttcagc
    18241 ggatcagctg ttttcaatga taaagatttt gagcgatact caaaatgaaa ttcgttttac
    18301 caatagtgcc gctatttatt tggaagtcgc tacagtcaaa cttgcacagc taccgtctgc
    18361 ttctgttcct tcagcagatg tagcatttgc agatgatcag atagtaaaac agctacaaca
    18421 agagattaag gaactaagaa acgaaatcaa ggaactacgc agtaatacag gaattgagaa
    18481 tacaaaagta cagccggtac agcaaacaaa tcataaaaaa cagacaagta cctatcgtgt
    18541 accgacggaa cgagtatata aagttctgcg ggaagcgaca agacagcatc tgaaccaagt
    18601 aaaagaagta tgggaagact tgctgatgag tctatctgtc actcagcgag caatgcttaa
    18661 agcaagcgaa ccagttgcag ctggacctac aggactagtg attgcgtttg attatgagat
    18721 tgtttgtcaa agagcagcaa atgacgaaga attgcagctt tcggtacaca acaacttaag
    18781 ccgaatgatc aaagattatg cgcctgatcc tgtttatatt acaagagaaa gctggccaga
    18841 acttcgaaga aattatttga cgcaggcaca agaagaggga acagaaaact cttttgcttc
    18901 tgaagactca gaaggagaaa tcgagttgct tcctcaagaa caagaagtac cagaagaaac
    18961 acttgtagta aaagcagagg aactgtttgg ctccgaagct gttacagtgg tagatgatta
    19021 ataaaaagga gtagataatt atgatgcgtg gaatgggcaa tatgcaaggc atgatgaaac
    19081 aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga gctgctaaat aaaacagagt
    19141 ttacaggtaa agccaccaat gaattggtca aagcggtttt tacaggcgat cgtcgcatga
    19201 aagatatcca aatcaatgaa gaagtcgttg atcctgatga tatcgagatg cttcaggatc
    19261 ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga tgtggaaact gagaaaaaat
    19321 tagggaaata cgcaaaaggt ttgccaggat tttagtttta aactgaagga gtaatgaata
    19381 tgcaatatcc agaaccaatt gcgaaactga tcgacagcta catgaaactt ccaggaatcg
    19441 gtcagaaaac agcgacaaga ctcgcctttt atacgatcga tatgaaagat gaagtagtaa
    19501 atgaatttgc taaatctttg ttaagtgtaa aacgcgattt gcatttttgt agtatctgcg
    19561 gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa tccttcacgt gatcgaagcg
    19621 ttatcctagt ggtcgaagag ccgaaagatg taatggcaat ggaaaaaatg cgagaatatc
    19681 acggacttta tcatgttctc catggtgtgt tatcaccgat ggaagggaca ggtccagaag
    19741 acatcaatat ttcttctctg ttgcagcggc tgcatgatgc agatgtaaaa gaagtgatta
    19801 tcgcaacaaa tgcaacaaca gagggagaag caacagctat gtatctctca cgtctgatca
    19861 aacccgcagg tatcaaagtg actcgtctag cacatggtct gtctgtaggc tctgatatcg
    19921 agtatgcaga tgaagttact ttattgaaag ctgtcgaagg aagacgtgag ttataaattt
    19981 ctaattaagt gttttttcag atacaatata aaagcagcac aagagatggg ggcaacaatg
    20041 tgaacggact atttattaca atcgaggggc ctgatggtgc aggaaaaacc agcatcttga
    20101 atgaacttta ccctcttttg aaagaagtag caaaaacaga aatcattcaa acaagagaac
    20161 caggaggcat tccgattgct gaagagatcc gagcagtgat ccttgatcca aaaaatgatc
    20221 ggatggatga acgtacggaa gcactgcttt atgcagcagc aagaagacaa catctagtcg
    20281 aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt atgcgataga tttgtcgaca
    20341 gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt taccgagatt gctcggttga
    20401 atgagtttgc tacagaaggt actacgccgg attttaccct ttatcttgac gtagattcag
    20461 acaccggatt aagaagaatc aagaaaaaca gacaaaatca aatcgaccgt ttagattcgg
    20521 aaggtttgga attccaccag cgtgtacgtc atgcttactt aaagctggca gaagagaatc
    20581 cggaaagaat ccacaaagtt gatgcaagaa aaagctttga agaagtgctg caaacaagtt
    20641 atcatacaat tattgaacag tatccgcaat tttttgaaaa ctaggaaggt gaacgtttat
    20701 gaaaattatt ttagcgatta tccaagacaa agacagcaac cgtttatcaa atgaattgat
    20761 tgatgcaaat atccgtgcca caaaactatc atcgacaggc ggcttcctta aggctggaaa
    20821 cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa gaagcattgg aaatcatcaa
    20881 aaaaacgtgt gaatcaagaa agcaatttgt atctacacct gttacactgg atatttcgat
    20941 ggatgggggc gtcccttatc cagtagaagt agaagtaggc ggtgccacag tcttcgtctt
    21001 acctgttgaa ggattccatc aattttagga ggagctgagc tatgaatcaa gaaataaact
    21061 tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg gagttttgag catgggcgta
    21121 tcgctcatgc ttatcttttt gaaggggaga aaggaacagg aaaacatgaa gtaggtatat
    21181 ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa tctgccttgt ggaaaatgca
    21241 acaattgtca gcggatcgaa aagaaagaac atccagatgt tttggttatt gaaccggaag
    21301 gacaaaccat caaagtcgat cagatcagac gtttgcagac ggaattcagt cgcagcggtt
    21361 acgaatcaag aaaaaaagtt tttttgatca aagaagctga aaaaatgaat agcagtgcgg
    21421 caaacagttt gttgaaattc ttggaagagc cccctggaga ttttttagcg gtgttagaaa
    21481 cagatgcagt aggaagaatt ctccctacta ttcaatctcg ttgtcaagtt ctgcattttc
    21541 aagaactttc gaaagaagca ctgatccatc gattgcaaga agagcagatt ccattggaaa
    21601 aagcaaaact tttggctttc ttaacaaaca gtttaggaaa agcagttgaa atctctcaag
    21661 atgaatggtt taatgatgct aaggacttga tttatcaatg gttcgtttac ttgcaaaagc
    21721 aagatactca agcctttatc tatgtccaaa aaaaattggt aaaatcattt aaagaaaaaa
    21781 cacaacagtt tacaggatta tctgtcttga tgttttatta tcaggaagca cagcgtcaag
    21841 cagtagcaaa cggcgaatat ggcaaagtga aacagataaa ccagataatt gaacggatat
    21901 tgatcgcaga gcagaaacta cgcagcaatg tcagttttca ggctgttgcg gaacagttcg
    21961 ttttgcatac gattttttag cttgccgcaa aggatgatga tcaaggagga tggaaatggt
    22021 agaagtagta ggtgtccgat atagagatgc aggtcatatc tattattttg caccaggaga
    22081 ctctgaatat gtttatagtg acaaggtatt agtcgaatct cagcaatcct tacatattgc
    22141 tactgttgtc cttcctaaaa aagagattga tcccaatgac cttcctgaag ctatccgtcc
    22201 gatcttgcat aaagcgtcac aagctgaatt agaaaaagac aaaaaaaaca aaaaagatgc
    22261 ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg cgtgagttgg aaatgaaaat
    22321 ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt ttttctttca cagcggacgg
    22381 gcgaattgac tttagagaat tggtaaaaga tctggccgct gtatttcgta ctcgtattga
    22441 gcttcgacaa attggtgtcc gcgatgaagc aaagataata ggcggaattg gtccatgtgg
    22501 gcgtccatta tgctgttctt cttttttagg cgatttcgtg cctgtatcaa tcaaaatggc
    22561 aaaagatcaa agtttatctt tgaatccaac aaaaatttcc ggtttatgcg gacgcttgat
    22621 gtgctgtctg caatatgaga gcgaagaata cgagacagcg aaaaaagaac tccctgactt
    22681 tggaaaagta attgctacac ctgatggtaa agggaaagtc gtagggttga atctgcttag
    22741 ccgtatcgtt agtattcggc tgactggacg ggaaaatcct gtcgaatatg aatgggatga
    22801 attgaaagaa tttatccaag taggtgagat gaatggataa gagatcatta tacgacggat
    22861 tgaatcaatt agaatcagag ttgagaaata cattaacaga acttgttgaa atgaagcatg
    22921 ctttgcatga gatcgtagaa aaaaatacaa ctttggagat ggaaaatcaa cgtctgcggg
    22981 aacacctgcg ggaattgaac caagttgcac aagctccagc tgaaaatgtg aagcaggaac
    23041 tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga aggattccat gtctgctacg
    23101 atttatatgg atcaagaagg gacaatgacg agccttgtgc gttttgtcta gaagtgatct
    23161 atcgcgaaag cggtcgatga ccaataacaa gaggagactg gagcggagaa atactgctcc
    23221 agtcttttta ttaaggagat tgtgacagat gtataatcaa aaaagtttca agggaaatcc
    23281 ggtatatggg gatttatatc ttgtaccgac gccaattgga aatttagaag atatgagcta
    23341 cagaagtgtt cgtatgctgc aagaatccga tcttatcgca agtgaggata cgcgtaatac
    23401 acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa aaaagtttac atgaacataa
    23461 ttataaagaa cgtgtccctc aattgattgc tttgttaaaa gaaggaaaaa cgattgctca
    23521 agtcagtgac gcaggaatgc cttccatcag cgatcctgga catgagctag ttttagcctg
    23581 cataaaagaa gggatttctg tgatctctat cccaggacct acagctggga tgacggcttt
    23641 gattgcttca gggctagtgc ctcaaccatt tttattttat ggcttccttc cacgtaagaa
    23701 aaaagaacag aaagaaacat tgaaaaagct aaaagaacag acagttacgc tgatttttta
    23761 tgagtcacct tatcggatca ctgctacatt gacgaatttt ttagaaattt ttggaaatcg
    23821 ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa gaatacctta gaggaagtac
    23881 ggaagagcta ctcgcttata tcgaagaaca tccagtaaag ggtgaatgtt gtctgcttgt
    23941 agaagggaat ttgttcgaaa tgccagtgac ggaagttgat gaagagttgg gaacgttaaa
    24001 ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca aatgctgcta tcaaagcagt
    24061 agcgctgaaa aatgggctga aaaaacaaga agtctaccgt cagtaccatg aattagactg
    24121 agatagtata agttgtctat aatgaaagac taatgaggaa atcagctact aacttttcaa
    24181 atctcttgaa cgcgaacgaa tgttcttgta taatgaataa gaggtgagga tgatggagct
    24241 gcaatttcct ttacataatg atacatcaag aaaaatcatt catattgata tggatgcttt
    24301 tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc ggacatccat tagtaattgc
    24361 cagacacccc agtgacactg gtgggcgtgg agtagtgact acagcaaatt atgaagcaag
    24421 aaaattcgga attcattcag caatgagtgc acaaaaagcg tatgagcttt gtccgcaagc
    24481 catctttaag cctggcgacc atcataaata cagcgatatt tccaaacaag tacgggaagt
    24541 attctatcgt tatacagata ttgtggaacc agttagtatc gatgaagcgt atcttgatgt
    24601 gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc gcccgaatga tccaagcaga
    24661 tatttgggag agtgtccagc taacatgttc tgcgggagta agttacaata agtttttggc
    24721 caaattagca tcagactatc aaaaacctag aggaatcacg gtagttgccc ctcaagacgc
    24781 tgtgccattt ttaaaagctt tgccaattga aaaatttcat ggggtaggga agaaaactgt
    24841 acctagaatg catgagctag ggatttatac aggaaaagac ctatatgaat gcacagaaat
    24901 gatgttgatt cgaaattttg gaaaaatggg ctattctctt tatcgtaaag tcagaggaat
    24961 ccatgattct ccagtaaacg ttacaagaga gcgaaaatct gtgggaaaag agcatactta
    25021 tgggcagccg ttgcaaacgg aagaagcagt gctgactcag ttgcgccaac tagctgaaaa
    25081 agtagaagaa gcactgagaa gagtccaaaa acatgggaaa acagtagttt tgaaggtcag
    25141 gtacacggac tattctactg tgaccaaaag agtgacattg ccagaatata tctataaaaa
    25201 agaagcactc ttttatcaag caagccttat atgggaggaa atcttgggag tagaaaaagg
    25261 aatccgattg ttagggatta ctttgacgaa tctggacccg atgacttatg aaaacatcgt
    25321 ccttccatta tgggaaaatc aagaaatata aaaacagctt tgagagttga aaccggaaat
    25381 ccgttttctc tcccaaagct gtttttatat caagtcttgc tttttgattt gttcataggt
    25441 ttctgttgca tccgtacatt tttcccaaat cacaacttga ttggattcta aagatttttg
    25501 gacgtcaatg atccgctggt tgctacttcc gcgaaattgt agttttaggt cacgtttgct
    25561 taattcaaag cgtccgtcaa caaggatatc gatttgcgaa aggagttcta atttatcttc
    25621 tgaatcttgt agcaattcat caaaggcata gcctgtccat gaccagatat cttttgtcgt
    25681 accgaactct tttcggatac gtcgtacgac ttgcagacaa acatctgtat tcaaaaaagg
    25741 ttcgccacca agtaaagtta atccttgcac gtaatcgttg cgcaagtcag cgatgatttg
    25801 ttcttctaat tcaggtgtga atggtttgcc atatcggaaa ttttgtgcag ctttgttgaa
    25861 gcagccttcg caggcaaata agcagccact aacgtaaaga ctgttgcgga caccttcacc
    25921 atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt tggctgtagt cttttgctaa
    25981 ccattctttt ggttttggat ttctcataaa aaaacttctt tcatttaagg ttcaaagata
    26041 tagctattga agataaaatc tactcgacca cataaataag aacaaactga cctaaatcca
    26101 ttgttcaaga aagcttgttc ttattttgat agagggcgtt caaagataca aagccatcga
    26161 aagagggtgg tgaaaaaaga cttgtcatag cctcttattc tgaataaacg gtgttcaaaa
    26221 gcttgcatct gcagtaataa gcccaactaa acgaaaaata tgaggaacta tttttgttta
    26281 gctattctta ttacttgatg caggacagct ttttcacaac ctcatttcat gtgtttcaca
    26341 cgtgaggaaa tttctttatg gcggccatgt accatcggac gagcttgcgg atttcctaaa
    26401 tagccacaag tacgtttgac tacatcacat gtttttggat catgatttcc acattctgga
    26461 cattcgaacc ctcgttctgt cggtttgaaa tcaccttcga aaccacattg atagcaatga
    26521 tcgattggcg tattcgtacc aagatacccg attttgtcat atgcaaaatc ccatacagct
    26581 tctaaagctt tcggattttg ttggagcata gggtattcac agtagtggat gaatccacct
    26641 gaacaatatt ttggataatc cttctcaaat tccaattttt caaatggcgt tgggttttta
    26701 cgcacatcat agtggaaact gtttgtataa tattctttat ctgtgatatt ttcgatgatg
    26761 ccgaattttt ctgtatccaa acggcagaaa cggtcagtta gactttcact tggagttgaa
    26821 tagacactga aatgataacc atattcatcc ccccatgcat ctgcattagc ttttaattct
    26881 ttgacgatat caagtgtgaa agcttttgct tctgggtttg tttcccattc gccaccgaag
    26941 aaagctgaag caacttcata caatccaatg tagcctaacg atacagtagc tcgacggttt
    27001 ttgaataatt cattgacaga atcggtacgt gaaagacgtt tcccgaaaga accatacata
    27061 tataaaattg gcgcatttgc tggaatggct tctttacaac gttctacacg atagagaagc
    27121 gcatctttca tgatttctaa gcggtcagac agtaactgcc agaatttatt ctgatctcct
    27181 tttgattcga tcgcgattct tggcaaattc aatgtaacaa cacctaaatt cattcgtccg
    27241 acatttactt cttgaccatt ttcgtctttc cacccttgca agaatgaacg acagcccata
    27301 ggtactttga aacttccagt taattcaacg attttatcgt aattcagaat atcaggatac
    27361 attcttttcg ttgcacattc caatgctagt tgtttgatgt cgtaattagg atcttcaggg
    27421 tttaagttca cacctttttt caaagtgaag atcagtttag ggaagatagc tgtccgcttt
    27481 tcactcccta atccattgat tcgaatctgt aaaattgctt tttgaatttc tctttcaaac
    27541 cagttttctc ctagtccaaa tcctaatgaa gtgaaaggag tttgtccatt tgaagtaaat
    27601 aaggtattga tttcatattc caaactttgc atagcatcga agatatcttt tttggttttt
    27661 gcttttgcat attctttttg gcgttctggc gaatcgatcc attcttccgc gtcttttaga
    27721 tgttttttat agttcaattc tgcaaaagga gcaagcaatt catctgttcg gtcggcagaa
    27781 cagccaccat attgacttga cgctacattg gcaataatct gtgagatttg tgctgtggcg
    27841 gtctggatag attttggcga ttcgacttcg gcattgccga ttttgaaccc attgttcagc
    27901 atgcctttga aatcaatcaa gcagcaattc gtcatcggtg tataaggatg ataatccaaa
    27961 tcatggtaat ggatatcgcc tttttgatgc gcatttgcta tatgtggtgg caacattttc
    28021 agtccgattg attttccaac gatcccggct gtcagatctc gctgtgtatt gaaaacatta
    28081 ctgtctttat ttgcgttttc gttcacgaca gtttggtcct tgttgattag cttttcaatc
    28141 gaaaagttga tatctgttgc tttactgcga gcaaaatctc gtttggtacg ataattgatg
    28201 tactcttctg ctaattcata ttcgtttttt tctagtaata tatgttccac gatgttttga
    28261 atctcataga ttttgacatt ttcagcaaac cggcttttga tttcttggac aaccaagtct
    28321 gcaatcattt gaattttttc ataagtaagt ggtgataatg agccgtgaat tttcttttct
    28381 gctttgatca gtgcttcatt gatcttttgt tcatcgaaat caacatggcg gccatcgcgt
    28441 ttgatgactt tcatctggga tgtctctggc tgctgagtaa cgacagtttt ttcttcataa
    28501 tttactttca ttatcttcaa ctcctgctaa attgttcttg tctatcataa aacaaaatcg
    28561 gggaaggagc aactaaatat cggctatata tagtattcaa tgtgctgtat aattggattt
    28621 ctatactata tgtagtgtat attttattgt gaaaaaaggt gaaaacttgt agctggtgga
    28681 tttgtttttt gcatatttaa ttttgctgaa taaaataatt gaataaaatt ttttattcag
    28741 tccaagtaaa agtgacaaaa acataagagc aaattattcg tagatatgct actatgcaaa
    28801 taaatagaaa tgaaaaatga aagaggagga gaagaattgg aaaagatgat cgaagtgaaa
    28861 catctaagta aaagctacgg cagcaaactt tctcccgtca aagccttaga taatatctca
    28921 ttcacagtag aagaaggcga gtttgttggg attatgggcc caagtggtgc aggaaagaca
    28981 acgctgatga atatcttgtc cacgatcaat ttgcctacga tgggcagtgt ctcgatccaa
    29041 ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg actttcgtcg aaaaaaacta
    29101 ggattcattt ttcaagagtt caatttgatc gataccctaa atgcaaaaga caatatccta
    29161 ttgcccctag cagttgaacg gatgactaaa gaggaaatgg aaaagagagt aagacatgta
    29221 gctcaattgt tgaatataga agaactgctc aaacggtatc ccgatgaact ttctgtcgga
    29281 cagagacaac gaattgcagc tgctagagca cttgttgttc agccacaagt gatttttgca
    29341 gatgaaccaa ctggctcttt ggactcgaag tcggctacag aattgttgaa ctacttgaag
    29401 accatgaatc aaaaagaaaa agcaacgata ctgttagtca cccatgatcc atatacagca
    29461 agctactgcg atagaatttt atttattaaa gatggtgtga ttttttctga agttgtcaga
    29521 agagggacaa gaagagagtt ctttgagaaa gtaatagaca tgcaggcgac gattggcgga
    29581 ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt ttttttcggc agtcacgagg
    29641 ctatcttgtt tattttttca gtttgacttt atccgtaatg atctattatt ccttcagcgc
    29701 aatgacctac gatcaatctt tagtcagaag agcaagtcaa gatacttcga tagatggtgg
    29761 attgagtttt ggtggattta tcattactgt tgttttgctt tttttcgtat tcagtgccaa
    29821 ccgatttttc ttgaatagaa ggcaaaagga aataggcatc tatcagcttt ttggtatgaa
    29881 taaactgcag atatcaggta tatatgtgtt agaaatcatg ataattggat tgttcgcctg
    29941 tatttctggg atattactcg gtattatttt ttccaaactc ttttcaatga ttttggttcg
    30001 aatgatggat atggatctta ctagtccatt ttttatctcc attccatcag tagtagatac
    30061 tttatttgct tttttcatca tccttttagc agtatctgta tattcaatat ggaaaatatg
    30121 gcgctatcca atgattcgaa gttttggaga aaaagaacaa gcagatagtt caacaatgcg
    30181 cttcaggaca aggcaccgat tactcgcagt catcgggtta ctacttttaa cgagcggata
    30241 ttttggagct agtcatttta gggaaatcac ttcatggatg atcagttatc attatcttga
    30301 tacattagtt ttcttttttc cttttttaat ttttttcgtt tgtgtggtcg gtacttattt
    30361 gtttttctgt tatacatttc ggttattttt gatcatattg agtaaatgga agatttatta
    30421 ctatggtatc aattttttga taatgggtaa cacgcaagtt catttgctca aaagttggcg
    30481 tacaaattct ctgattacag tcattctcgg aatttcttta atgacaattg gcggcacggc
    30541 tagtatctct actattttat cctaccagga gaaagtttcc tccccgatgg actatcagtt
    30601 tgatgttgag acagagaaaa aaatcaagcc gattctagca gaggaagggc agaagataac
    30661 taaagaaatc accttgcatt ataaagtcat tgggagtatc tatagtcaaa atctcgaaga
    30721 acctatgttt caaactgcta atctgatcac ggaaacacaa tatcaagcct tcagaaaagt
    30781 taatccagat ttgccgaaca tttctttgaa atcacctggt catactgttt tgcttgatca
    30841 aatgcaaact ctttttcgga atatttcatt atatggatct agtattggac tgccagataa
    30901 tcaagaattg aagattcaac aaatgcttcc aagtgtatta ggtgacgaaa caatggtata
    30961 tctaagtcct gtactgattg tagatgatac cgtttttgaa aaagcaaaag gtgtggatta
    31021 tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa aaaatagatg aacgactgag
    31081 caatgacctg tcgataaagt ggctcaatgc tgtttattat tcttatgata tcgtaaatgg
    31141 aagtctacaa ggagaaatta gcacaaagaa acttccggat gaccaaatga aggaagcaca
    31201 atttagccaa gaaagctctc gtttgaattt ctcttccagc tattcgaaat tgcgtgcagt
    31261 taatcggcga atcggtatca atatttttgt cacactattt gtcgggatga tcgtgattat
    31321 tgctactggt agtattttga cagtccggca attagcagaa gcagaggaag aaaaagaaaa
    31381 ttatcaccta ttgactaagc tggggattcc acaaagaagg atcagacgga tgatcttcga
    31441 gcaaaacgca cttaccttct ttccacccat gatattaggg atcactcacg cagtatttgc
    31501 aatcaatgtc ttcactcagt atataaaaac tgccgattac tggttggctt atcttgtgag
    31561 tggcttattg atccttatct atcttttgct ttatgtcatt acgagtcagt tatattgtcg
    31621 gattatcgag gaatgatata cggaataaga caagataata aatagaacta aattttgaat
    31681 accatttatt tggaataaga ggttgtgaca cgatttttgt cacaacctct ttatttttta
    31741 tattttttaa aagtacgaga aattataaag aaaaaagcag tgatttgatg tgctattttt
    31801 ataaaagcta tgtgacaaa
//
LOCUS       DACDCV010000021        31271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDCV010000021 DACDCV010000000
VERSION     DACDCV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.021"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IV041_000995"
     CDS             173..1309
                     /locus_tag="IV041_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8381804.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IV041_000996"
     CDS             1446..1790
                     /locus_tag="IV041_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8381805.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IV041_000997"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IV041_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8381806.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IV041_000998"
     CDS             complement(3485..4084)
                     /locus_tag="IV041_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8381807.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IV041_000999"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IV041_000999"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8381808.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IV041_001000"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IV041_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8381809.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IV041_001001"
     CDS             7290..8408
                     /locus_tag="IV041_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8381810.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IV041_001002"
     CDS             complement(8468..9760)
                     /locus_tag="IV041_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8381811.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IV041_001003"
     CDS             9910..10470
                     /locus_tag="IV041_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8381812.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IV041_001004"
     CDS             10599..10976
                     /locus_tag="IV041_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8381813.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IV041_001005"
     CDS             10909..12981
                     /locus_tag="IV041_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8381814.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IV041_001006"
     CDS             complement(13192..14139)
                     /locus_tag="IV041_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8381815.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="IV041_001007"
     CDS             14385..14849
                     /locus_tag="IV041_001007"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8381816.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="IV041_001008"
     CDS             complement(14911..15954)
                     /locus_tag="IV041_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8381817.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="IV041_001009"
     CDS             complement(16331..17296)
                     /locus_tag="IV041_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8381818.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="IV041_001010"
     CDS             17530..18375
                     /locus_tag="IV041_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8381819.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="IV041_001011"
     CDS             18566..19753
                     /locus_tag="IV041_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8381820.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="IV041_001012"
     CDS             19759..20115
                     /locus_tag="IV041_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8381821.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="IV041_001013"
     CDS             20117..20455
                     /locus_tag="IV041_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8381822.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20850..21884
                     /locus_tag="IV041_001014"
     CDS             20850..21884
                     /locus_tag="IV041_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381823.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="IV041_001015"
     CDS             21877..22563
                     /locus_tag="IV041_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381824.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="IV041_001016"
     CDS             22587..23435
                     /locus_tag="IV041_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8381825.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="IV041_001017"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="IV041_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8381826.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="IV041_001018"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="IV041_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8381827.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="IV041_001019"
     CDS             25701..26936
                     /locus_tag="IV041_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8381828.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="IV041_001020"
     CDS             26923..27393
                     /locus_tag="IV041_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8381829.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="IV041_001021"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="IV041_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8381830.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="IV041_001022"
     CDS             complement(28884..30026)
                     /locus_tag="IV041_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8381831.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="IV041_001023"
     CDS             complement(30065..30799)
                     /locus_tag="IV041_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8381832.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..>31271
                     /locus_tag="IV041_001024"
     CDS             31031..>31271
                     /locus_tag="IV041_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8381833.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKS"
BASE COUNT        10170 a         5731 c         6596 g         8774 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct g
//
LOCUS       DACDCV010000022        31003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDCV010000022 DACDCV010000000
VERSION     DACDCV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.022"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..777
                     /locus_tag="IV041_001025"
     CDS             <1..777
                     /locus_tag="IV041_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8381834.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            926..1264
                     /locus_tag="IV041_001026"
     CDS             926..1264
                     /locus_tag="IV041_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381835.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1469..2044)
                     /locus_tag="IV041_001027"
     CDS             complement(1469..2044)
                     /locus_tag="IV041_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8381836.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2776..3357)
                     /locus_tag="IV041_001028"
     CDS             complement(2776..3357)
                     /locus_tag="IV041_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8381837.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3540..4166)
                     /locus_tag="IV041_001029"
     CDS             complement(3540..4166)
                     /locus_tag="IV041_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8381838.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4188..5519)
                     /locus_tag="IV041_001030"
     CDS             complement(4188..5519)
                     /locus_tag="IV041_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8381839.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5674..6075)
                     /locus_tag="IV041_001031"
     CDS             complement(5674..6075)
                     /locus_tag="IV041_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8381840.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6161..6367)
                     /locus_tag="IV041_001032"
     CDS             complement(6161..6367)
                     /locus_tag="IV041_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381841.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6462..7964)
                     /locus_tag="IV041_001033"
     CDS             complement(6462..7964)
                     /locus_tag="IV041_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8381842.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7982..8437)
                     /gene="argR"
                     /locus_tag="IV041_001034"
     CDS             complement(7982..8437)
                     /gene="argR"
                     /locus_tag="IV041_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8381843.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8535..9152)
                     /locus_tag="IV041_001035"
     CDS             complement(8535..9152)
                     /locus_tag="IV041_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381844.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9373..10746
                     /locus_tag="IV041_001036"
     CDS             9373..10746
                     /locus_tag="IV041_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8381845.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10821..11933
                     /locus_tag="IV041_001037"
     CDS             10821..11933
                     /locus_tag="IV041_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8381846.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12005..13642)
                     /locus_tag="IV041_001038"
     CDS             complement(12005..13642)
                     /locus_tag="IV041_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8381847.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13810..14418)
                     /locus_tag="IV041_001039"
     CDS             complement(13810..14418)
                     /locus_tag="IV041_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8381848.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14802..15953)
                     /gene="queG"
                     /locus_tag="IV041_001040"
     CDS             complement(14802..15953)
                     /gene="queG"
                     /locus_tag="IV041_001040"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8381849.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15972..16724)
                     /locus_tag="IV041_001041"
     CDS             complement(15972..16724)
                     /locus_tag="IV041_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381850.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16865..17815)
                     /locus_tag="IV041_001042"
     CDS             complement(16865..17815)
                     /locus_tag="IV041_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8381851.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17816..18655)
                     /locus_tag="IV041_001043"
     CDS             complement(17816..18655)
                     /locus_tag="IV041_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8381852.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18652..19398)
                     /locus_tag="IV041_001044"
     CDS             complement(18652..19398)
                     /locus_tag="IV041_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8381853.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19636..21006
                     /locus_tag="IV041_001045"
     CDS             19636..21006
                     /locus_tag="IV041_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8381854.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21061..21957)
                     /locus_tag="IV041_001046"
     CDS             complement(21061..21957)
                     /locus_tag="IV041_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8381855.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22238..22915)
                     /locus_tag="IV041_001047"
     CDS             complement(22238..22915)
                     /locus_tag="IV041_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8381856.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23006..23911)
                     /locus_tag="IV041_001048"
     CDS             complement(23006..23911)
                     /locus_tag="IV041_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8381857.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24086..25540
                     /locus_tag="IV041_001049"
     CDS             24086..25540
                     /locus_tag="IV041_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8381858.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            25556..26587
                     /locus_tag="IV041_001050"
     CDS             25556..26587
                     /locus_tag="IV041_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8381859.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26594..27781
                     /locus_tag="IV041_001051"
     CDS             26594..27781
                     /locus_tag="IV041_001051"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8381860.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27937..29319
                     /locus_tag="IV041_001052"
     CDS             27937..29319
                     /locus_tag="IV041_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8381861.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29291..29677
                     /locus_tag="IV041_001053"
     CDS             29291..29677
                     /locus_tag="IV041_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381862.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29877..30545)
                     /locus_tag="IV041_001054"
     CDS             complement(29877..30545)
                     /locus_tag="IV041_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8381863.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(30734..>31003)
                     /locus_tag="IV041_001055"
     CDS             complement(30734..>31003)
                     /locus_tag="IV041_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8381864.1"
                     /translation="QMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHG
                     KVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         8732 a         6312 c         5669 g        10290 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      841 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      901 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      961 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
     1021 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
     1081 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
     1141 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
     1201 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
     1261 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
     1321 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
     1381 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
     1441 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
     1501 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
     1561 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
     1621 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
     1681 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1741 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1801 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1861 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1921 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1981 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     2041 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     2101 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     2161 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     2221 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     2281 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     2341 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     2401 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     2461 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     2521 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     2581 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     2641 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     2701 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2761 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2821 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2881 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2941 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     3001 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     3061 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     3121 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     3181 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     3241 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     3301 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     3361 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     3421 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     3481 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     3541 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     3601 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     3661 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3721 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3781 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3841 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3901 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3961 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     4021 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     4081 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     4141 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     4201 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     4261 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     4321 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     4381 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     4441 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     4501 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     4561 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     4621 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     4681 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4741 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4801 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4861 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4921 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4981 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     5041 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     5101 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     5161 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     5221 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     5281 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     5341 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     5401 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     5461 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     5521 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     5581 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     5641 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     5701 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5761 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5821 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5881 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5941 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     6001 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     6061 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     6121 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     6181 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     6241 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     6301 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     6361 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     6421 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     6481 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     6541 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     6601 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     6661 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6721 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6781 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6841 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6901 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6961 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     7021 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     7081 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     7141 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     7201 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     7261 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     7321 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     7381 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     7441 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     7501 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     7561 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     7621 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     7681 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7741 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7801 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7861 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7921 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7981 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     8041 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     8101 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     8161 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     8221 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     8281 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     8341 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     8401 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     8461 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     8521 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     8581 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     8641 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     8701 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8761 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8821 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8881 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8941 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     9001 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     9061 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     9121 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     9181 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     9241 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     9301 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     9361 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     9421 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     9481 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     9541 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     9601 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     9661 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9721 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9781 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9841 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9901 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9961 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
    10021 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
    10081 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
    10141 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
    10201 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
    10261 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
    10321 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
    10381 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
    10441 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
    10501 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
    10561 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
    10621 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
    10681 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10741 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10801 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10861 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10921 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10981 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    11041 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    11101 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    11161 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    11221 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    11281 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    11341 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    11401 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    11461 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    11521 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    11581 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    11641 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    11701 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11761 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11821 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11881 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11941 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    12001 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    12061 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    12121 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    12181 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    12241 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    12301 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    12361 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    12421 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    12481 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    12541 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    12601 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    12661 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12721 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12781 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12841 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12901 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12961 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    13021 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    13081 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    13141 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    13201 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    13261 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    13321 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    13381 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    13441 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    13501 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    13561 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    13621 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    13681 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13741 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13801 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13861 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13921 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13981 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    14041 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    14101 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    14161 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    14221 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    14281 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    14341 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    14401 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    14461 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    14521 ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata caaaaccata
    14581 aaagccttgt tgtataagca aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa
    14641 aacgcagatt ctgtaacatt cactgcaata atccataaaa aaaagcaaaa aaatttttga
    14701 tttttatcaa attctcttaa aaaaatgtaa aaaatacaaa aaaaccagta attgttacaa
    14761 caattactgg acaaaaactt tgaataatgt taactatttt tttattttaa ctttctccgc
    14821 aataatttct gggcatgttc caattcgttt ataacgactt cttctgtttc tttagctaat
    14881 atttcatcta atacatcaag ccatttttcc ggttcttttg ttcctagacg tccaatagcc
    14941 caagctgctg ttcctctgat gacagggcgg acatctgtta aacattctga aatctcaggc
    15001 aatgccgttc gatcacctag attagctaaa gcaatcaatg cgtttctctg caaaggtttc
    15061 ttcccacgcc atgaaccagc taaatgccca aattgctgtt tgaagtcttt attagacatc
    15121 gaaagcattg gctttaattt tggatatact tcgtctactt ttggttccat ctcttcatga
    15181 aaatgaaaat cttttccttg attgtaaggg cagactaact ggcagatatc acatccataa
    15241 atgacatttc gcattttttt acgatattct tccggcatca tcccttttgt ctgagtttga
    15301 taagacaaac atcgctgggc attcattctt ccgtcaccaa gcaatgcttt tgtcggacaa
    15361 gcagtcacgc agcgcataca gtcaccgcaa ccaaattctc ctggtttatc aggctcaaat
    15421 acaatattag taacgatctc accaagatag acaaatgaac cgaactcttc agtgatcaac
    15481 aatccgtttc gtccaataaa tcctaaacca gctcgctgtg ctacagcgac atcgatcagt
    15541 tctccagtat ctacttgcgg agctagacgc cattcctctt ctttttgcca tttttctgct
    15601 cttgtttgga tgaattcgat caatcggttc aaacggtctc tgagtatatc atggtaatcg
    15661 atcccccaag aagcacgagc aaactgcccg cgtttttcat ccctaggcac ttcttcatgg
    15721 atcctagtag gatacgcaag tgcaatggaa atgatcgatt tcggctgatc aaatgtcatt
    15781 tctggataaa gacgctcatc gatatttgga tgttcaaagc cagaagtatg gccagctgct
    15841 ttttgttctt ccaacgattc ttttaatgaa tcaaaaggtt ccgccgtggt gaacccgatt
    15901 ttgtcaattc ctaatcgttt gctttcttgt atgatttctt cttttaatgt catggtcttc
    15961 ctcgcttgct gttaacggct gtataaccca taatatagtt tttctttttt cagctggaca
    16021 aactggatct gctgatcggc aatcttcttt tcttccatcc atttttttct tgcagcaaat
    16081 acttcccaat aatttgttga aattaatttt cttttgttat ctaatgcatt aaaaaaattc
    16141 aatgaaactg gttttgtact taacagttca tggagcctta cctcttccca tggcgaaaac
    16201 tcagacagat cgcgggaata caatacttct tcatagagat ttttttgctg tgtgtacgtg
    16261 aatgtttgcg ggattttccc aggcctagca atttctacta gacgattata aaacatattc
    16321 attaatttgc tgttggcaat aaattgactt ttataatagg ccgactggat ctttttgatt
    16381 actccttctg ctttgatgac tgaaaataat gcaggccatg ctttgacggt tatttcgtcg
    16441 ttttgtttaa ctaatacgcc aggtgtgaag ccgactggag aaaggaacac aaaataaccg
    16501 ataccggtat tttcatctaa aggctgaaat tcatcaaaca accatttgcc tatgtacagc
    16561 tcttcatctg gttctgttgt ctcattttga tttgctatga ataatttagc taattctgct
    16621 tcaagaaatt gaaggtcttg atccttgtcg gtattttcct gctggctgat caatcgaaag
    16681 acttcttctt taatcgtata tttatcttta cgtgtaaaat tcatagccac accttccttc
    16741 cttactctaa ctataacgga atttcttcaa aaaaggtatc tgtacgacga aaaacagcat
    16801 aatttttttt catatctctt ataaaaaatg caaggtctgc tcctcaagct agaccttgca
    16861 cttttcagct ttcttttgtt tgtgacaaac cttcatggat cgtttcaata ttccatttca
    16921 tcatctgata gtaagaatct ccttgttctc cttcttttgc tatggaatca gtgaacagtt
    16981 tatcatagat tttcaaaccc gtttcttttg ctacccgttc catgcttctt gagtctacac
    17041 tggtttccac gaataaaacc ggtacttctt ttgctcttat ctgatcaatg atcgctttca
    17101 tttgatcagg cgtaccttga ctttctgtat tgatctccca tatataagct gctggcaaat
    17161 catatgctcg tgaaaaatac ttgaatgccc cttcacttgt tactaataat tttttgttct
    17221 cttcgataga agcaaaactt tccttcgctt gtgtatctaa ttcttttagt ttttccacat
    17281 atgcttctgc atttttttta tagagtgtcg cattttctgc atctttttca gagaatatac
    17341 gtgcgatttc ctccacatat tttattccgt tagataggtc tagccaagca tggggatctg
    17401 cttttgtatg ttcttcaccg ctagttaaat aaagaggttc tacatttttg ctaactgcaa
    17461 aataatcttt tccttcttct tttttagccg tttccatcaa gttatcgaac cagctgttac
    17521 ctgtttcaag attcaaaccg ttgtataaaa taacatctgc atcgcttgcc tttttgatgt
    17581 cttctggtag tacttcatat tcatgcggat ctgttccgac tggtacgata ctgtggatat
    17641 ctatttgatc tgttccgacc tcttttgcca tgtccgccaa gatcgaattg gtagccacga
    17701 ctgacaactt ttctttactt ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg
    17761 ctaataccaa gaaactaact gccaagatta aaaagagtga tttttttgtt ttcatctatc
    17821 tgcttccttt ctgaaataaa attccttttg ttggagaaaa taaaaaagca aggataaaaa
    17881 atgcggtaca tgctaacacg atcgtagctc ctgatggcca attgtaacta taactgaaaa
    17941 acacaccgac aactgcactt agcataccga ttcctgcagc tgtaatgatc attttcagta
    18001 aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat caacatggca atcaccaaga
    18061 ttgttcctac tgtctgtaaa ctgactacag ccactaatgt caaaaagaac atcaaaagat
    18121 aatggatcag cgatgtgttc agcccataag cttttgccat tgtcggatcg aatgatgtga
    18181 tcttcaattc tttataaaag aaaaagacaa atatcaagac gattccacta acgagtgctg
    18241 tcagcaacaa atcgctttcc ctaacagcta agacattccc aaataaaata tgatacaaat
    18301 ctgtcgcact ctgggcatat gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga
    18361 caattccaat cgctgtatca tttttcaatc ggctccgctg agtcacgaaa ccaatcactg
    18421 ctgctgttag catcccaaaa gcagtggctc caaagatgta actaaaacca aacatataag
    18481 aaatggctac acctggaaga actgcgtgag agatagcgtc tcccatcaaa gacatccctc
    18541 gtaaaataat aaaagaaccg atgactccag aagcaagtcc tacaagcatg gaagtgacca
    18601 aagcgtattg caggaattga taattcatta atccatcaat aaaggattgg atcatttttt
    18661 ctctcctcct tttactaaat gtctgatgat ctcaccatag gccaattgaa ggttcttcga
    18721 tgtaaagact tcttgtactg gaccggaagc gatcagctgt ttatttaaaa gaataacctc
    18781 atcgaaatat tcttctactt tatgaagatc atgatggacg atcaaaatcg tttttcctga
    18841 attcttcaat tcctgcaaga tgtcaaagat ctttcgttca cttaacgcat caatccctac
    18901 gaatggttca tctaaaaaga tccattctgc tccttgggct agagctcttg caataaaaac
    18961 tctctgaagc tgtccacccg atagttcgct gatctgtctt ttggcatatt cttccatccc
    19021 tacttttttc aaagcttgtc ttgcacgttc tttttctttt ttcccaggtc tttgtccaat
    19081 tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat acatcaattg gaaaggaaag
    19141 atccaattca cttcgctgtt ccacgtaggc tattttttta cggactgagt ttaccggctg
    19201 tccagaaaag gtgactttac cagtagtagc tggtatcaac tctagcaaag atttcataaa
    19261 tgttgatttt cctgcaccgt ttggtccgat aataccggta attttttgtg gatttatcgt
    19321 caagttgata gttttcaaaa cagttttccc ttgataagcg gctgaaaggt cttgaattac
    19381 aatggccgta cgttccaaat cttacactcc tttttaggtt tacctaactt tttatttaaa
    19441 tatagtttaa ctaaaatgga aagcattttc aagtagaatg atgaaaaagt ttttttcgaa
    19501 ttttcctcgc attttatgaa aattttctgt agaatcacat caagagttca ataaggctaa
    19561 cctaataatg aaaaacatgt gttgaaacgt ttgattattt tttcttgttg actcaacaac
    19621 gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc
    19681 caaatgatcg cactaggtgg aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc
    19741 atacgttgga cagggccctc agtgatggga gcctatgcga ttgcaggatt ctttttgtat
    19801 ttgattatgc gtgctttagg cgaaatgctt tacgttgatc cttcgactgg ttcttttgca
    19861 aattacgcga gcgagtacat ccatcctgtg gctggctact tgaccgcttg gagtaatatt
    19921 tttcaatata ttgtcgtagg aataagtgaa gtgattgctg taggatctta tatgaattat
    19981 tggtggcctg atttgccggc aattattccc ggaatcattg tcgtcttgtt tcttatgctg
    20041 gcaaatctta tttctgtcaa ggcgtttggc gaattagaat tttggttttc gatcatcaaa
    20101 gtcataacga tcattttgat gatcattgca ggtcttgggg taatcttatt tggatttggt
    20161 aatcatggtc aggcagtagg gatttcaaat ctttggaaaa atggtggatt cttcactgga
    20221 ggcgtcaaag gatttttctt tgcattatcg atcgttgttg cttcctatca aggaatcgaa
    20281 ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa agacgatcat tgaagctgtc
    20341 cagtccacta tcggtcgaat cttgattttt tatattggtg ctatttttat cattgtgagt
    20401 atctatccgt ggaatcaatt gagtgagatt ggttcacctt ttgtacaaac tttttctaaa
    20461 gtcggcatta catttgctgc aggactgatt aattttgtcg tgattacagc ggcattatca
    20521 ggttgcaatt cagggatttt cagcgcaagt cgtatggtct atacacttgc cataaatgga
    20581 aagatgtcta aaaaatttct gaaactatct cgtcacggtg cgccttttta tcctgtcgta
    20641 gcaatctcag gtggaatctt aattggtctg atcttaaatt atcttctacc aatgttcatc
    20701 aaccagtcag aaaatatttt cgttttcgta tacagctcaa gtattttgcc tggtatggtt
    20761 ccttgggtag tgattttgat cagcgagatt cgcttcaaaa agaagcacgc agataaaatg
    20821 catgatcatc cttttaaaat gccactatct ccttatacga attatgtgac acttgctttt
    20881 ttagctatcg tattgatttt catgtttatc aatcctgaaa cgagaatctc attgattatt
    20941 ggactcgttt tcttgcttta catgacgatc cattactttg tacgcgaaaa aagaaccgga
    21001 aaataaaaaa agaacgaagc ttcaccttag tggaagcttc gttctttttt attgctttta
    21061 tcaattcaat tttagcgtct caccgggagt caggaaataa ttctccatgc tcatcccatt
    21121 cagcgacaag atcgtttcta ctggtacgcc agttcgttcg gctatttgat ttggaccttc
    21181 tccttccaaa actacggttg tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga
    21241 ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca gaagcttcta ccgactctgc
    21301 tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc
    21361 gctagtctgc aaataaatat cggaaatctt agcgacatga ttcgtataat tagccgataa
    21421 ttgattgatc aaagtatcta agtctacacc gtctttattt tctgctgcct gatacgctgc
    21481 acgtaatttt ctgatccctt cgtcggagat tgcttgaagc tcacgtgctt tttggttagc
    21541 aactgctgat aaaccagcta ttccctcttg atttccctga atttctgtct ggtattcttc
    21601 aatcaatgca ggcgtctttt cttcgatctt tttcgaataa tcatttacga ttgcttgaag
    21661 ttgctctggc aaaccatttg ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt
    21721 tgatgactca cttgtttgtt tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt
    21781 acttttcgct tctgacgaat tttttccatg aaaatataga cccgcaactc ctcctgctat
    21841 gatcaaaaac acggcaactg ctgccccaat catgatcatc cgcttacgag ctgcctgttt
    21901 ttgctgtctt cttcgggtca tccgaacatt ttctccccac tcattttcat catacatttt
    21961 tttcaccacc aatttttatg tataatagcg ttatcaaacg cttcatattc ttattgtagc
    22021 aaataattgt cgctgtgcac atttttatct ttgttattta taagaaaatt tcaccagttg
    22081 agatgattcc aagattgtcc gtcgatttct ccttgcagct gcattgttcg ttcgtctatc
    22141 tcagccatca gtttctttat ttcatataag agcgcacggt cttgatattc tgatttttcg
    22201 cttaattcat ctaattgttg gatcagccaa tcatttgtca taatgaagtt cctttcattt
    22261 cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc ctcagtcagt gcttctacga
    22321 tatatgcttg tttttcagat tcttcttcct gcattttctg attttcctgt actctttgaa
    22381 taaaccagtc aatccgctga gtcagcgatg gtttgccttc caatgagaga tactgctgat
    22441 aagcaattag cttctcccaa cgtttttcct gggataaata cataaattgt gctaaaacga
    22501 atggcggaag ataggtcata tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt
    22561 cggaaatcgt atattcttct tttcctccag cttggtattc tttttccgcc acacctgttg
    22621 tcaatacgat tcctagttct ttccctttta atacatgtgc tgctcgttcc aagacattat
    22681 cttgccaaat ttttaaatga gcaggagaac tataccagta aaacggaaac tggaaaatga
    22741 tccgatcatg ctgggccaag agctgtagtt cagtctctat atctattttt ccgtctggat
    22801 aacttgattc caaatgatgc caagtagctg gtaaaccagc agaagcttct ttgaaaaatt
    22861 gctgatttaa agattcttcg atcgttggat ggctaatgat gataagtgta cgcatccctt
    22921 ttcctccttt ttagtcttcg ctttacagtg tagcataacc gccacaagtt tcttttgccg
    22981 atgcttgtca ttttaaccag actaatcagg ttgtccaaga aactgcggtt tgtttttcca
    23041 actgtttttt caaggtatcc aaagcaatat tcttaaaatt ttcgtttttc atacgtttac
    23101 gatagttcgt agccgtttct ccggtactct ttttgaacat tcggctaaat acgactagac
    23161 tattgaaccc tacttgatag gcaatacttt ccacggattc atccgtcaga gaaagaagaa
    23221 acgcccctcg attgatccgt accccgatca aatattcctt gatactggta cccatatgat
    23281 ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa atgctgggcg atttcctcaa
    23341 cagacaattt ttcagcataa tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg
    23401 aatgaacctt ttgtacacca ccgctgacct ttccgccatc ttttaaaagg actgataaaa
    23461 aacggtaggt taatgctgtt aactgtaatt cattttccaa ggtttcttcc gtatctttca
    23521 tgcattctaa aataatgtcg atatattttt gtatgtcaga agagaccatc gtgtattggt
    23581 cttctctgaa aagcgactgc cgaatgattt cttctgcagc actcccacca aatgaaagcc
    23641 agcaatacat ccacggatct tctccatctg ataagtaaaa cgtcgagtct cctggacgaa
    23701 tcaagaacag atcacctttt ttcaattgga attgttgatt tttcacatga taagatcctt
    23761 tgccttccat cacgacatgc aggatgtaat cattgcgaat agcaggacca aaagaatgaa
    23821 acggcatcgt tttagagaaa ccacaaaaat caaaatacaa ttctttttct acttgttgat
    23881 gttccttttt taaaaaaatc gattgctcca cgtctatccc tccttactcc tttagtataa
    23941 tgcaacaaaa agttaaataa aagtcacaga aatatattaa aaaggaagcg atttcttgtt
    24001 aagtttaagt cattaaaaga tctttgttat tcgttgtaac cggtttactt agtattatat
    24061 attgaacagg agaggaatac agattatgaa aagcaaaatg atccaacgtg tgtcctacgc
    24121 tttaggcgct tttggacacg acacattcta tgttacatta agcacttatt ttatggtatt
    24181 cgtgacaagc cagatgttcg caggaacaga taaggcaacg aatgcaaaaa tgattgctac
    24241 agttacaagc ttagttgtag gtatccgctt agttgaaatt atttttgatc ctttgattgg
    24301 tggcatcatc gataatactc gtacaaaata cggtaaattc aaaccatggt tagttatcgg
    24361 cggcgttgtt agttccgtac tcttggtagc aatctttacc aatttctttg gcttagctac
    24421 tcataatacg actttattta tcatattatt tgcaatcgtt ttcatcttat tggatagttt
    24481 ttattcattt aaggatatcg ctttttggtc aatgattcca gctttgagta cggaaacaaa
    24541 ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct tctctaggtg caaatggtac
    24601 tactctagtc gttgtcccta tcgttacttt ctttacctat cttgttactc ataaacatat
    24661 tgaaggcgct tcaggctggt tctggttcgg tgtgattgct gctgttattt caggcggtac
    24721 tgcgattatc actgccttgg gcacaaaaga agtcgatact gcgatccgtc agcaaaatga
    24781 aaaagctggg atcaaagatg tgttcaaagc agttgctcaa aatgatcagt tgatgtggct
    24841 tgcccttgct tatatcgctt atgcgattgc caatgtagca acaactgccg tattattcta
    24901 cttcttcaaa tatgtacttg gccaaccaac tgaattttgg atcgttggtc tagttgctgc
    24961 cttacttggt ttagtagctg ttccactatt cccaatgctt acacgcgtca tctctcgtcg
    25021 ctacgtttac cttggaggga tcgcaatgat gatttcttct tactttttct ttacgatcgc
    25081 aggttcaagt ttgccaatcg ttatcatcgg gctagtattg ttctacttcc ctcaacaatt
    25141 gattttctta tccgctttga tgacgatcac agactctgtt gaatatggtc aatggaaaaa
    25201 tggtatccgt aacgaagctg ttactctatc aattcgccct ttactagata aaatcgctgg
    25261 tgctttgtct aacgggatcg tagggtttgt cgctatcgct tctgggatga ctggtaatgc
    25321 tactgctgct gatatcactg ctcatggcgt aacgactttc aaagcttatg cattctatgc
    25381 tcctgctgct ttgatgattt tctccgctat tatttttgct tggaagatta cattgacaga
    25441 gaaaaaacat gcacaaatcg ttgaggaatt ggaaaacaaa ttggctccag ctgatacaat
    25501 ggaagatgaa cttcacgatg cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa
    25561 gattacagaa aaagattttg gccaaggtta tcacttgata accttggaga acaaaaacaa
    25621 gctagcactt tctatctctg atttaggtgc tagaatcgtc agtttaaaat caaatgatcg
    25681 agaattagtt ttgggatttg acaccgctga agaatatatc gaaaaagatc cgtatatcgg
    25741 tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc cgcttttctc tcaatggaaa
    25801 aacttaccaa ttagccacag atccaaaaac tggacataat ctgcatggtg gtgctcctgg
    25861 atttgaatta aaaaaatggt cttatgtcat tttgaatggc gaaaatgaag cttctgtcat
    25921 ttttactact acaagtcccg acggagaaca tggttttcca ggtacaatgg atgtggaaat
    25981 ccgctatact ttgacaaaag ataatatctg gcgagtgacc tctcgcggca ccagtgacca
    26041 agatacttta ttcaatccga caaatcatgt ttacttcaac ttgacgggag atgcttctca
    26101 gtcaatcgat caacatgagc tttggctcaa tagcgaagct tatgctcctt tgcgaacaga
    26161 tagtattcct ataggggtaa aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc
    26221 taaaaaatta gcgtccgttt ttgctagtga tttggatcaa aaaaatcttg ttgacggaat
    26281 cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa gaagccgctc gcttgactag
    26341 ccccgataaa agaatccaag taggtattgc tacagatgct tctagtgtgg ttattttcac
    26401 tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga aaactggcac atcacggtgg
    26461 catcacattt gagactcaaa cagctccagg tgcagagcgc ttctcagcat ttggttcgat
    26521 tcatttaaaa gctggatcag tatttgaaac agtgacagaa ttcaaaatca aaacaagaaa
    26581 ggaataactc atcatggaaa tcaaaagaag cttaaacaca aaatttgccg aaacattcgg
    26641 acctaaaaaa actacacaat acttctcacc aggacgcatc aatttgattg gtgaacacac
    26701 agattacaat ggaggccatg tcttccccgc ttccatcact tatggaacgc aaggtgtagc
    26761 agcacctcga gacgataata aaatattagt ttattccaca aactttgacg atatcggtgt
    26821 cattagtttt actttagatg agctagaata tgataaaaaa gcaaactggg caaattacgt
    26881 aaaaggaatg gtccttaaat taaaagaagc tggacactca atcgatcatg ggtttgagtt
    26941 gttagtggaa ggaacaatcc ctaatggtgc aggactttcc agtagcgctt cattagaatt
    27001 attagtaggt gttgtattag aagatctgtt tgatttaaaa acagatcgct tggaactagt
    27061 ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc aattcaggta tcatggatca
    27121 gtttgccatc ggcttcggtg agctagatca tgcaatctta ttagatacaa atacgctgaa
    27181 atacgaaatg gttccagtga aactagatgg ttatgcaatc gtcatcatga atacgaataa
    27241 gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt
    27301 ggcacgtttg caaaaaactt tgcctatctc ttctttagga gacttagatg aagaagaatt
    27361 tgaatcaact attgaccaaa tcggcgatga tacattgata cgcagagctc gccatgccgt
    27421 ttctgaaaat cagcggactt tgaaggcaaa agctgaatta gaagctggga acttagaagc
    27481 atttggtcaa ctgttaaatg attctcacca ctctcttcgc tatgattatg aagtaactgg
    27541 gatcgaatta gatacactag tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg
    27601 aatgacagga gctggatttg gtggctgtgc gattgctctt gtcaaagaag aaaatatacc
    27661 tgccttcaaa aacaatgtct atgatgaata catgaaagtt gtcggctatg ctccggaatt
    27721 ttatgttgca cacattggaa atgggacaac aaagatcgat ccagaagctg ttcatgtttg
    27781 ataaaaaaat caacgatctc agctaaaaac aaatagataa aaataacctc tcagcaattt
    27841 ccaaatgtga gccttttgct cgtatttgaa aattgctgtt ttttattgac acggaaagag
    27901 aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt gggcgaatca aaatccggta
    27961 tttgtttatg aaaaagaacc ttcaagagat attttatgta ttgactgcaa atctttttac
    28021 gcctccgtag agtgcgttga aaaggatctt gatcccctta caactaaatt agtagtcatg
    28081 tcctatccgt ccgattcgac agaaacacgg ggtagcggat taatcttggc ttccagtcct
    28141 gcagcaaaaa aagcttacgg catcaccaat atcagccgtg ccagagatct tcccttcccc
    28201 tatccaagtg atttatatat tgttgcccca aggatggcct attatatgga aaagaacaga
    28261 gaaatcaatt ctatctataa aaaatatgca gacgaagcga accatcatgt ctattccgtc
    28321 gatgaaagct tccttgatgt aacaaatagc atggcactct ttggtaaaac aaatgcgtac
    28381 gacatggcta aactgatcca taaagatgtg tttgagcaga ccggaatcta cacgactata
    28441 ggaattggcg ataacccgct acttgctaaa ctagccttag acaatggctc aaaagacgga
    28501 aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag aaaagttatg gaaaatctca
    28561 gatatcactg acttttgggg aatcggcaaa cgaacagcaa aacgtctgaa acaaatgggt
    28621 atacattctg tttatgattt agctcacgcg gattattatc acttgaaaag caagctaggg
    28681 gtcatcggta ctcagcttta tgcccatgct tggggcatcg atcgaagttt tttgggacaa
    28741 gaatatacac caaaatcaaa aagtatcgga aatagtcaaa tcctagatag agattataca
    28801 agacgagaag aaatcgagat cgttatcaaa gaaatggccg accaagtcgg cactcgcttg
    28861 agacgatctg cagcaaaaac acaagtagtc agcttatggg taggatactc tttaggctat
    28921 cttgatccaa atggcaagac cgggtttcac cagcaaatga aaatcgaagc gacaaatgct
    28981 agcagagatc tagcagatgc tttattgacc attttccacc agcattacca ctaccaggat
    29041 attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg cgacaggttt acagcttaac
    29101 ctttttgaag atcctgaaaa gcagataaat gaagcgaaag ttgattttgt tgtcgatacg
    29161 attagaaaaa aatacggctt tcaatcaatc atccatgcgc atagtctctt gcaaggagga
    29221 cgcgccatcg cacgtagttc tctcgttggc gggcatgcag gtggtatgag tggattagaa
    29281 ggtggaaaag atgagacgga cagcaaaaaa attcattgac tataacgagt atcgagatcg
    29341 gccatttgga ttaaaatggg ggactgcttt tgcgatggaa gaactagtca acggtatcaa
    29401 aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga cattgcaaga
    29461 aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca ttcagctcaa
    29521 tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca ttggtgaagc
    29581 ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg tacggtatat
    29641 tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag aagtgatcaa
    29701 cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt ggaattttgg
    29761 cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga ggttgtgaaa
    29821 aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct gtctatctat
    29881 ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta gagccctttt
    29941 tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc tttcttcaag
    30001 ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg ctgaatttgc
    30061 ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt caatacactc
    30121 atttgaccag cattctctag gataacatgt ttggcatttt caagagaaaa gcattcttgc
    30181 tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg
    30241 ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg attgaccttt
    30301 tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag cgcaatggca
    30361 aataggacat gccctacatg gacattgtca tcataggttg attcttctaa gatcccacct
    30421 aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt cttcccaagc
    30481 aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt aattgaaaaa
    30541 atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc tcacgaacac
    30601 gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg tgacactgta
    30661 gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt ttttatgctc
    30721 taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg agactttgcc
    30781 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
    30841 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
    30901 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
    30961 agattttgta ctccttcaat ttgtttctat aagctttcat ttg
//
LOCUS       DACDCV010000023        30520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDCV010000023 DACDCV010000000
VERSION     DACDCV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.023"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..315)
                     /locus_tag="IV041_001056"
     CDS             complement(70..315)
                     /locus_tag="IV041_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381865.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(431..1009)
                     /locus_tag="IV041_001057"
     CDS             complement(431..1009)
                     /locus_tag="IV041_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8381866.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1086..1616)
                     /locus_tag="IV041_001058"
     CDS             complement(1086..1616)
                     /locus_tag="IV041_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8381867.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1620..2480)
                     /gene="rlmB"
                     /locus_tag="IV041_001059"
     CDS             complement(1620..2480)
                     /gene="rlmB"
                     /locus_tag="IV041_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8381868.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2473..2868)
                     /locus_tag="IV041_001060"
     CDS             complement(2473..2868)
                     /locus_tag="IV041_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8381869.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2865..4277)
                     /locus_tag="IV041_001061"
     CDS             complement(2865..4277)
                     /locus_tag="IV041_001061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8381870.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4274..4810)
                     /gene="cysE"
                     /locus_tag="IV041_001062"
     CDS             complement(4274..4810)
                     /gene="cysE"
                     /locus_tag="IV041_001062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8381871.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5132..6592)
                     /locus_tag="IV041_001063"
     CDS             complement(5132..6592)
                     /locus_tag="IV041_001063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8381872.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6762..7919)
                     /locus_tag="IV041_001064"
     CDS             complement(6762..7919)
                     /locus_tag="IV041_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8381873.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7953..9323)
                     /gene="radA"
                     /locus_tag="IV041_001065"
     CDS             complement(7953..9323)
                     /gene="radA"
                     /locus_tag="IV041_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8381874.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9386..9913)
                     /locus_tag="IV041_001066"
     CDS             complement(9386..9913)
                     /locus_tag="IV041_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8381875.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10143..10949
                     /gene="proB"
                     /locus_tag="IV041_001067"
     CDS             10143..10949
                     /gene="proB"
                     /locus_tag="IV041_001067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8381876.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10946..12193
                     /locus_tag="IV041_001068"
     CDS             10946..12193
                     /locus_tag="IV041_001068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8381877.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12269..12613
                     /locus_tag="IV041_001069"
     CDS             12269..12613
                     /locus_tag="IV041_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381878.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12730..13056)
                     /locus_tag="IV041_001070"
     CDS             complement(12730..13056)
                     /locus_tag="IV041_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381879.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13671..14873)
                     /locus_tag="IV041_001071"
     CDS             complement(13671..14873)
                     /locus_tag="IV041_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8381880.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14990..15505
                     /locus_tag="IV041_001072"
     CDS             14990..15505
                     /locus_tag="IV041_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8381881.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15704..16150)
                     /locus_tag="IV041_001073"
     CDS             complement(15704..16150)
                     /locus_tag="IV041_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8381882.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16197..17675)
                     /gene="msr"
                     /locus_tag="IV041_001074"
     CDS             complement(16197..17675)
                     /gene="msr"
                     /locus_tag="IV041_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8381883.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(18029..18658)
                     /locus_tag="IV041_001075"
     CDS             complement(18029..18658)
                     /locus_tag="IV041_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8381884.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18642..19106)
                     /locus_tag="IV041_001076"
     CDS             complement(18642..19106)
                     /locus_tag="IV041_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8381885.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19593..20861)
                     /locus_tag="IV041_001077"
     CDS             complement(19593..20861)
                     /locus_tag="IV041_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8381886.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(21092..21829)
                     /locus_tag="IV041_001078"
     CDS             complement(21092..21829)
                     /locus_tag="IV041_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8381887.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21896..22465)
                     /locus_tag="IV041_001079"
     CDS             complement(21896..22465)
                     /locus_tag="IV041_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8381888.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22593..23465
                     /locus_tag="IV041_001080"
     CDS             22593..23465
                     /locus_tag="IV041_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8381889.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23807..24679
                     /locus_tag="IV041_001081"
     CDS             23807..24679
                     /locus_tag="IV041_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8381890.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24676..26634
                     /locus_tag="IV041_001082"
     CDS             24676..26634
                     /locus_tag="IV041_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8381891.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26966..27817)
                     /locus_tag="IV041_001083"
     CDS             complement(26966..27817)
                     /locus_tag="IV041_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8381892.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27940..28407
                     /locus_tag="IV041_001084"
     CDS             27940..28407
                     /locus_tag="IV041_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8381893.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28670..29152)
                     /locus_tag="IV041_001085"
     CDS             complement(28670..29152)
                     /locus_tag="IV041_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8381894.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(29252..30115)
                     /locus_tag="IV041_001086"
     CDS             complement(29252..30115)
                     /locus_tag="IV041_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8381895.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8730 a         6457 c         5516 g         9817 t
ORIGIN      
        1 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
       61 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
      121 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
      181 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
      241 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
      301 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
      361 tcaagtttac cataaaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
      421 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
      481 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
      541 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
      601 cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt tcttgcaatg
      661 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
      721 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
      781 ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
      841 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
      901 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
      961 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
     1021 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
     1081 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
     1141 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
     1201 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
     1261 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
     1321 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
     1381 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
     1441 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
     1501 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
     1561 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
     1621 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
     1681 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
     1741 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
     1801 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
     1861 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
     1921 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
     1981 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
     2041 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
     2101 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
     2161 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
     2221 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
     2281 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
     2341 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
     2401 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
     2461 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
     2521 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
     2581 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
     2641 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
     2701 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
     2761 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
     2821 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
     2881 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
     2941 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
     3001 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
     3061 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
     3121 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
     3181 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
     3241 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
     3301 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
     3361 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
     3421 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
     3481 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
     3541 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
     3601 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
     3661 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
     3721 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
     3781 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
     3841 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
     3901 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggt
     3961 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
     4021 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
     4081 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
     4141 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
     4201 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
     4261 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
     4321 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
     4381 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
     4441 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
     4501 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
     4561 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
     4621 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
     4681 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
     4741 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
     4801 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
     4861 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
     4921 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
     4981 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
     5041 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
     5101 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
     5161 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
     5221 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
     5281 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
     5341 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
     5401 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
     5461 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
     5521 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
     5581 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
     5641 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
     5701 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
     5761 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
     5821 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
     5881 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
     5941 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
     6001 aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg atattatcgg
     6061 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
     6121 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
     6181 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
     6241 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
     6301 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
     6361 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
     6421 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
     6481 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
     6541 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
     6601 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
     6661 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
     6721 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
     6781 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
     6841 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
     6901 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
     6961 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
     7021 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
     7081 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
     7141 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
     7201 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
     7261 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
     7321 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
     7381 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
     7441 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
     7501 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
     7561 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
     7621 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
     7681 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
     7741 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
     7801 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
     7861 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
     7921 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
     7981 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
     8041 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
     8101 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
     8161 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
     8221 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
     8281 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
     8341 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
     8401 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
     8461 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
     8521 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
     8581 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
     8641 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
     8701 ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg gctgacactt
     8761 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
     8821 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
     8881 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
     8941 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
     9001 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
     9061 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
     9121 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
     9181 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
     9241 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
     9301 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
     9361 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
     9421 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
     9481 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
     9541 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
     9601 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
     9661 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
     9721 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
     9781 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
     9841 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
     9901 ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg
     9961 ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt
    10021 aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt
    10081 tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc
    10141 acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat
    10201 tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt
    10261 ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg
    10321 gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg
    10381 cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc
    10441 aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa
    10501 atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa
    10561 acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc
    10621 tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg
    10681 acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg
    10741 ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag
    10801 gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga
    10861 ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag
    10921 gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa
    10981 agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca
    11041 aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt
    11101 agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc
    11161 tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat
    11221 tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt
    11281 gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga cagatgcagc
    11341 tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt
    11401 ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc
    11461 ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt
    11521 gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa
    11581 agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga
    11641 taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc
    11701 atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt
    11761 gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc
    11821 gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct
    11881 agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa
    11941 tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg
    12001 tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga
    12061 cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg
    12121 tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca
    12181 aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa
    12241 ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta
    12301 ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca
    12361 attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat
    12421 cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt
    12481 agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact
    12541 gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg
    12601 gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac
    12661 tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac
    12721 ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc
    12781 gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt
    12841 ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc
    12901 agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc
    12961 tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag
    13021 tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct
    13081 taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag
    13141 ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca
    13201 actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt
    13261 ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaatcag
    13321 ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt tccgttttgt
    13381 ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg tgttcaatca
    13441 gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt
    13501 ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg gtgttcaaaa
    13561 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tttttgtttg
    13621 gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc ttattcttct
    13681 tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat aagaacaatg
    13741 gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac acctgcaata
    13801 aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga agcaacgttt
    13861 cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat aattccttgg
    13921 aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt tccagctcct
    13981 aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg ataagtcgta
    14041 aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag cacaccccag
    14101 ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa tggcgcatac
    14161 gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac tgaacgaagt
    14221 ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt tggtttctca
    14281 gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa tgctacccca
    14341 aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg gccaacagcc
    14401 attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt accaactaaa
    14461 atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac accccagccg
    14521 gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc agcgaataca
    14581 acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact agataagaaa
    14641 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    14701 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    14761 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    14821 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    14881 tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt aaaacgtgga
    14941 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    15001 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    15061 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    15121 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    15181 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    15241 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    15301 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    15361 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    15421 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    15481 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    15541 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    15601 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    15661 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    15721 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    15781 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    15841 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    15901 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    15961 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    16021 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    16081 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    16141 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    16201 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    16261 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    16321 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    16381 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    16441 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    16501 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    16561 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    16621 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    16681 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    16741 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    16801 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    16861 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    16921 acactgtctt tttgtttaga ggaagacaac cgatccagtc tgatttgttg ctttttcttt
    16981 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    17041 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    17101 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    17161 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    17221 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    17281 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    17341 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    17401 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    17461 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    17521 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    17581 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    17641 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    17701 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    17761 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    17821 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    17881 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    17941 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    18001 tagctactga atagtaactg aaaacctttt attcgaacat agtgatcttt tctggtgcta
    18061 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    18121 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    18181 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    18241 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    18301 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    18361 ggttgtagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    18421 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    18481 cttcctcgct ttcagcgacc acaaagcgcc atggtttcat attgacagaa gaaggtgcag
    18541 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    18601 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    18661 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    18721 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    18781 tctttatggt ccatttcaca atgcttgatt ttcttctggg ctttttctgt tagcgctaca
    18841 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    18901 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    18961 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    19021 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    19081 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    19141 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    19201 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    19261 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    19321 gatagctctt ctgcagtaga taattttaca ccgccaaaaa tttccgaaat cgccttttgc
    19381 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    19441 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    19501 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    19561 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    19621 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    19681 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    19741 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    19801 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    19861 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    19921 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    19981 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    20041 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    20101 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    20161 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    20221 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    20281 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    20341 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    20401 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    20461 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    20521 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    20581 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    20641 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    20701 gactgcaaag tactttctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    20761 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    20821 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    20881 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    20941 ttaaataaca tttgtttctt tttaataaat ttgctttttc tgcataaaaa gacgtaaatg
    21001 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    21061 gtaattctct ctggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    21121 tcatgttgct gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    21181 tatcttcaga tgttaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    21241 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    21301 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    21361 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    21421 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    21481 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    21541 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    21601 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    21661 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    21721 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    21781 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    21841 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    21901 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    21961 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    22021 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    22081 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    22141 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    22201 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    22261 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    22321 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttccg
    22381 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    22441 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    22501 cggcttatga tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    22561 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    22621 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    22681 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    22741 gaggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    22801 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    22861 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    22921 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    22981 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    23041 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    23101 atcggaatca tcctttagca aagcaaacag tcatccatcc gcaggaactg aaaactgttc
    23161 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    23221 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    23281 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    23341 aaaaagtatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    23401 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    23461 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ttaagcaaaa
    23521 gatccaatga ttaaataaca taagtttctt aatttgttcc ttccttttat ttctgataat
    23581 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    23641 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    23701 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    23761 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    23821 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    23881 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    23941 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    24001 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    24061 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    24121 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    24181 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    24241 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    24301 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    24361 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    24421 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    24481 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    24541 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    24601 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    24661 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    24721 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    24781 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    24841 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    24901 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    24961 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    25021 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    25081 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    25141 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    25201 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    25261 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    25321 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    25381 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    25441 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    25501 atgttagcag cagcttttgc ttcattcttt actggtgtaa cagaaccact tgaattctca
    25561 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    25621 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    25681 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    25741 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    25801 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    25861 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    25921 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    25981 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    26041 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    26101 catggtggtg ctgcaacacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    26161 gccggtggaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    26221 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    26281 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    26341 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    26401 aacactgttg acttaaaagg cgaaccattc actttatatg taaaagaagg tcaaaaagtc
    26461 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    26521 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    26581 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    26641 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    26701 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagcc
    26761 aaacgtaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    26821 gcttttttac aacctctgat aaacggtgtt caaaaaccag tttatcggta tgaagtcaaa
    26881 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttcatactc aaaactaatc
    26941 ggctttttca caacctcttt atacattatt cctgatccaa tatttctttt aatgctgctt
    27001 caagagaagt taatggtttt cccaatactt tttctaaatc atcagaggaa atagctaact
    27061 gatccttttt aatatcatgt tggatcgcta aaaacatgtc agctgcttct tgagggaatc
    27121 cggcttcttt tagattttcg acaaacgctg catcacttgc ttcaataatt tctaactttt
    27181 tacctgtcgc acgctctaat gccttggcta attcttcata gtcagttaga ttgccggata
    27241 attctaagat ttctggagag tcactttcta ctagtacttt tgctgcagct tctgcatatt
    27301 cacgtttcaa tgcccaacct gtttttccat ttcctgcagc ataaacgaat tgaccagttt
    27361 ttaaagcctg cccaatgata ggtaattcat tctctacata ccaattattc cgtaaaaatg
    27421 tatgggaaat acctgaatcc ttaatcattt tttcagtgaa ttggtgatct ggcgctaata
    27481 cactgatcga gtgatcggca tctgcaaaac ttgtataagc gatataagat accccagctg
    27541 cttgtgctgc cttcactaca ttcttatgtt cggcttgacg atttcctgga acgccagaga
    27601 caaaaagcaa tcgatctatt cctttcaacg cttctttcat tgagtctaaa tcatcataat
    27661 caccaatacg tatattaaat ccagcttctt tcagtgcttc tcctttttct tcactacgta
    27721 ctagtacata gatttcattt tgtggaacca tcttttttaa gaattccagc gcataaccac
    27781 caaaacctcc agttgctcct gttactaaat aatgcatatt tgccactccc tattcttttt
    27841 atttgttaag atttacagct gtacatttat aaattacacc tttatcataa agagtgtggt
    27901 aagataagtc aagcaaaaaa aatgaaagga tgacaactga tgaagtattc tctgcaattt
    27961 agtgatgcaa tccatgtact tgcctatatt gaaatgtttc aagatacccg atcactttct
    28021 agtgagatga ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa aatcatgagt
    28081 cagctcaaaa aatccggttt gatctttaca aaaaccggaa aaccgcagcc ttcccttgcc
    28141 aaaagtccag atcagatttc tttactagat gtctatcgca gcatcgaagg taatacacag
    28201 cttatccaag tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa tatccaagaa
    28261 gctttagaag aaagctacgc tcggctgcag aaaaaggcag aagaagaaat ggctaagatc
    28321 acacttgata gtctgataca taaaatagcc gttttggaaa aagaaaaaag accagaaaac
    28381 acaggcctcg tagagagatt tttataattc tctctactgt gcctgcattt ttctgatcgg
    28441 aatcgtctac ataaattctt ttattctccg ggggacaaaa gccttgtcat gccccttttt
    28501 cctctctaat gctcaggtca aagcgctgaa gtcatgacgt cttgataaac ggtgttcaag
    28561 tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat ttcccatttt
    28621 tcctctctaa tgctcaggtc aaagcgctac tttcacgacc tctatattat catggtttat
    28681 tgactaccgg tatccagatt tctcccatat atttcccgtt ggtaactccc atttcgacta
    28741 cagcatttgg acccccaaca taggcgtaat cttctacttc tcttaaaaca tgcccaaagg
    28801 ccgtattcgt caaatcattt cttaattctt caggcgattc tgctaaagcg attacaacaa
    28861 gatacgttcc ttttgggaat gggatgattc gggtagtgtt tggaacatcc tgactcgttt
    28921 gaacaccgac atagtacatc attttcccgt tatagggttc gttgactgta aagaaataat
    28981 gattttcagc agcttgtttc aatgcatcta ttgttccatt accgataact ttttgctcaa
    29041 atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt gctactcttc aattccacac
    29101 caatacccgt caccgtaaaa tctggttttt cttcgatcct atactttgcc atttctccca
    29161 ccctttctat tatattaagt tcatcttaca taaaattaaa gggaaatgac acgattacgc
    29221 tcttttttct agggatattt ttatttttta actatttttt tgctttcact gcaatccaaa
    29281 tctcactgta aacattactg aaatcctctc cccattttgt aaaagagatc tcgggagctt
    29341 cgactaactc ataatctgaa gaaggaagcc attctgcata gattttcccc caagtatctt
    29401 gtaaagtttg gggaaatggc cctttattag gaaaaatggc ccaagtgtgt tcttctatag
    29461 caagcatttc taaatcagcg tacggatttt cttttgtcgt agcaaagcca atcatgtgca
    29521 tcaaactgcc cttttcttcc aagcgggctc catcaaatcc ataagaaacg ttgattactt
    29581 gatttggata taaatctcct aacttatgca tctcttccct ttgagtctcc gtgatcgtct
    29641 gcgctaactt ttgtatttct tgattgactc cttcaaattt gacaggaact tttttcgcca
    29701 cacctgccac ataaaactgt tctttctttt cgatacgaaa ttccatcgat tttcctcctc
    29761 tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat ctgctgattt
    29821 ctgaaggcag atatcctgac caatcacgaa aagctcttga aaaaccttcg actgtttcat
    29881 atccatatct aaacgccgtt tctgtcactc ttgatccatc taggagatcg cgattagcca
    29941 aagccaatct acggtttttg atatagtcca ttagcgacat ccctgcaata aacgtaaaga
    30001 tgcgttttaa ttgatattca gagattcctg tattttcggc tattatttta taggaaagtc
    30061 cttctgtcaa atgttcttcg atgtaatcga tcaattggtt cagatcttta agcatattta
    30121 tcttcctcct tgcttgcctt tttatcatat tatcaaagaa agtcaattac ttgatgaatc
    30181 aggaaccaat ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa ttctcaagca
    30241 gcgtttcgta acggataatt ggtattatct gcctatctat gttttacctt tagaaaaata
    30301 aattttgggg aagacacatt ctaagtaaac aaagcggtaa aaatatcttt tattccctta
    30361 gttaatccgc ttactaccta ctgtacaaag caaattgttg ttcaaactgc tgatcagaac
    30421 gctgcttgga tttttctttg cgatacatca tacctctttt atcaattgga acaattattt
    30481 atttctaaca aaatcacttc atttttgtca gataaataaa
//
LOCUS       DACDCV010000024        30094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDCV010000024 DACDCV010000000
VERSION     DACDCV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.024"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(391..1335)
                     /locus_tag="IV041_001087"
     CDS             complement(391..1335)
                     /locus_tag="IV041_001087"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8381896.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1631..2575)
                     /locus_tag="IV041_001088"
     CDS             complement(1631..2575)
                     /locus_tag="IV041_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8381897.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2796..4010
                     /locus_tag="IV041_001089"
     CDS             2796..4010
                     /locus_tag="IV041_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8381898.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4032..6047
                     /locus_tag="IV041_001090"
     CDS             4032..6047
                     /locus_tag="IV041_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8381899.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6130..7503)
                     /locus_tag="IV041_001091"
     CDS             complement(6130..7503)
                     /locus_tag="IV041_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8381900.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7529..7813)
                     /locus_tag="IV041_001092"
     CDS             complement(7529..7813)
                     /locus_tag="IV041_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8381901.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7815..9830)
                     /locus_tag="IV041_001093"
     CDS             complement(7815..9830)
                     /locus_tag="IV041_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8381902.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10009..10314)
                     /locus_tag="IV041_001094"
     CDS             complement(10009..10314)
                     /locus_tag="IV041_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8381903.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10403..11434)
                     /locus_tag="IV041_001095"
     CDS             complement(10403..11434)
                     /locus_tag="IV041_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8381904.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11641..12516
                     /locus_tag="IV041_001096"
     CDS             11641..12516
                     /locus_tag="IV041_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8381905.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12668..13510)
                     /locus_tag="IV041_001097"
     CDS             complement(12668..13510)
                     /locus_tag="IV041_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8381906.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13602..15479)
                     /locus_tag="IV041_001098"
     CDS             complement(13602..15479)
                     /locus_tag="IV041_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8381907.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15476..16897)
                     /locus_tag="IV041_001099"
     CDS             complement(15476..16897)
                     /locus_tag="IV041_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8381908.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16900..20289)
                     /locus_tag="IV041_001100"
     CDS             complement(16900..20289)
                     /locus_tag="IV041_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8381909.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20546..21937
                     /locus_tag="IV041_001101"
     CDS             20546..21937
                     /locus_tag="IV041_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8381910.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22010..22657)
                     /locus_tag="IV041_001102"
     CDS             complement(22010..22657)
                     /locus_tag="IV041_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8381911.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22665..23351)
                     /locus_tag="IV041_001103"
     CDS             complement(22665..23351)
                     /locus_tag="IV041_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381912.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23357..24430)
                     /locus_tag="IV041_001104"
     CDS             complement(23357..24430)
                     /locus_tag="IV041_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8381913.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24652..25326
                     /locus_tag="IV041_001105"
     CDS             24652..25326
                     /locus_tag="IV041_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8381914.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25331..26671
                     /locus_tag="IV041_001106"
     CDS             25331..26671
                     /locus_tag="IV041_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8381915.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26820..27806)
                     /locus_tag="IV041_001107"
     CDS             complement(26820..27806)
                     /locus_tag="IV041_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8381916.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28530..29561
                     /locus_tag="IV041_001108"
     CDS             28530..29561
                     /locus_tag="IV041_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8381917.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29670..>30094
                     /locus_tag="IV041_001109"
     CDS             29670..>30094
                     /locus_tag="IV041_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8381918.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8924 a         5633 c         5402 g        10135 t
ORIGIN      
        1 tcaaaaatcg tcatttttcc atcaaaatct gcttatgcaa aattataaaa aaattttgca
       61 gtttttctta ctttttttgt cgtttaattg aatgataaaa accagtttct ttattcagtt
      121 aatcaaatat gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt
      181 aattaaccac agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc
      241 atatgttcat atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt
      301 gcttacctat tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt
      361 ttaggatttt tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa
      421 agcacttgtt gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac
      481 ttcacgaaca ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc
      541 gtattcacca gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc
      601 ttttacgata cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat
      661 gatctcataa gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat
      721 acgatggtct ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca
      781 tgcagcaact tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc
      841 aacttttaat ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt
      901 accgataaca cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc
      961 tactgggttt gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac
     1021 gatttggcgc atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt
     1081 ttggttgata ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc
     1141 tgaccataca gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc
     1201 cccttctgct ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt
     1261 gatcattgca taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg
     1321 acttgttttt ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa
     1381 gtatagtctt gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt
     1441 ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata
     1501 tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca
     1561 acgatataat aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa
     1621 gattttcttc ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga
     1681 ataatttgaa taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg
     1741 aaaaactcgt ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt
     1801 cttgaaagga agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc
     1861 caaaatgagc tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta
     1921 atactctaag catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta
     1981 ataaatgagt caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat
     2041 gactgtatgt atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg
     2101 tttttccgcc gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta
     2161 tcaaaaaaga agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata
     2221 aggtttcttc ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc
     2281 tttgagtagc ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc
     2341 cattaataat ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg
     2401 tatgctcggg aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa
     2461 atagatcccc tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat
     2521 tgatattcat tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa
     2581 atgtctaatt cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt
     2641 attagttcac gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc
     2701 tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta
     2761 tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga
     2821 aattttctct gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa
     2881 ttcccagtat gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga
     2941 ctgctccctc ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa
     3001 tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc
     3061 ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct
     3121 ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac
     3181 gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat
     3241 ttgttgcagg acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca
     3301 gcttacctat tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc
     3361 aatcgcatgg cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt
     3421 atggtatttt tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca
     3481 cagtcctgac gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca
     3541 tgcaattggc aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc
     3601 gctatatgct tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg
     3661 tgataaacct gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat
     3721 tcattcctta cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca
     3781 atacttcaat cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc
     3841 tctttctctc ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta
     3901 tcttgctgtt gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga
     3961 cagtgataag agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga
     4021 ggtcgtgtaa aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact
     4081 ttgagatccg ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc
     4141 cagcgtactg gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc
     4201 ttgatcaacc ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg
     4261 gacaaaaaca tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta
     4321 tctttgtagg acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat
     4381 cagatattgg ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc
     4441 tttatgtcga tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc
     4501 cagtcactga aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg
     4561 gctgtgtaga actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag
     4621 agtccgatgt agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa
     4681 gaaaaatgaa agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg
     4741 gaatggttca acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt
     4801 caagcgattc ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat
     4861 gtagtgattt gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg
     4921 cgtatcaatc attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa
     4981 cgatattagc attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact
     5041 attcgatctt gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat
     5101 ttttacgcat cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga
     5161 atgaattagg gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa
     5221 tggataaaga aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca
     5281 tgatcacttg cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc
     5341 atctctatca agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg
     5401 aagaacttgg cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg
     5461 cgaatatttt cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa
     5521 agaatgtctt attgaatgac actatcacac agattactac tccatatttc aaattctatg
     5581 aacaagatgc tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt
     5641 attggggagg gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc
     5701 aaacaggaaa tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg
     5761 catggggagc aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa
     5821 agccaggtta tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt
     5881 gtacgctacc tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga
     5941 ttacttctga tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag
     6001 caaaacagcc acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc
     6061 ttgcgacata tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt
     6121 gaagtttgct taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa
     6181 gctagataat acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc
     6241 gtattctcct agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt
     6301 agaagaagcg attttaagat ctcctaatac tggaaggata aataatggca gccaactaat
     6361 caataagcta ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc
     6421 gaagacacca gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt
     6481 agtttgcaag cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa
     6541 gccaattaat acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt
     6601 agaatcgggt acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac
     6661 cattcgaacc ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag
     6721 tgcaaagatt acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc
     6781 taacaaagca agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc
     6841 tcgcaaaaag ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc
     6901 aaaaaacttt ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc
     6961 aacttgattg tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt
     7021 taatagtgtt ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc
     7081 cgcgcttact aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag
     7141 aaaaatgaat ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag
     7201 catgatcaaa gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt
     7261 gctaggaact actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag
     7321 tgaattactg attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat
     7381 agttccttgg atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat
     7441 caaagaaacg aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc
     7501 cattgatagt tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt
     7561 cttttacttc ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag
     7621 aagttagcgt atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata
     7681 cacttccaac atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa
     7741 gtgcttgttt gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg
     7801 caaaaatttt catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt
     7861 gatgcgtgta ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc
     7921 aaagcccgaa gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga
     7981 tgggctggat tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact
     8041 tcgtttacgc catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata
     8101 aacgcaccat actgctccac gcgctcgatc atagctttga tataactttc ttctatttta
     8161 ttttctgata atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga
     8221 tccgataatt ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc
     8281 ttcttttcta aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct
     8341 acgccttctt ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc
     8401 attgaaaatc ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc
     8461 aagtcagtca tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga
     8521 ggtacagtag aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct
     8581 gaattagtct ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc
     8641 aatgaagagc taatgccact tggacagacg atcacggctc ttatttttct atttctttct
     8701 tctgcgtccg cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct
     8761 tcagggatgc tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg
     8821 tagatggaag catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa
     8881 taggaaatac gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga
     8941 aacgaagcaa attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat
     9001 tccagcaaaa aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa
     9061 agagagaaaa atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact
     9121 tgtcgactaa tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc
     9181 atggatgttt ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt
     9241 cgatttggtg cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact
     9301 ttggcgtaat aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca
     9361 agtgactgca tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac
     9421 cctagcagcc taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat
     9481 tcatttctta tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct
     9541 tgaaaatgaa aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct
     9601 cttcgctctt gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc
     9661 cagtcgattg ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa
     9721 ggagttaatt gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct
     9781 agactgattt ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc
     9841 acctcctact tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact
     9901 tacagttgga ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa
     9961 aagcaggtga ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc
    10021 atgtttattt tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc
    10081 gatatctttt tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga
    10141 accagataaa gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc
    10201 ttctttgtct tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt
    10261 aggggccatt gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt
    10321 tcctcctagt agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac
    10381 aaacccgatt cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc
    10441 cttcttccaa aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc
    10501 cattaactaa tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa
    10561 ttactttttc ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt
    10621 cgggcaaatc gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt
    10681 cttgtcgatc ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg
    10741 gacgttcttt gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa
    10801 aatccagatc gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc
    10861 tgatatcatc tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc
    10921 catataaata gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga
    10981 aataatcata atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt
    11041 tttggactgt caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac
    11101 ctttttcttt tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt
    11161 tctctactag tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt
    11221 gactaggtgt agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct
    11281 gatcagtata ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt
    11341 ttttgatatt gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac
    11401 tctttccatt gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt
    11461 ttcagtatat cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt
    11521 cagaaaaaca cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa
    11581 tcaaaaagat agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc
    11641 atgcttattg aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt
    11701 gtcgattaca tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca
    11761 aaagctacgt atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc
    11821 ggatttaaag aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc
    11881 aatcaaacga ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca
    11941 aagaaaatgg cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat
    12001 gaagatttag atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca
    12061 aaaggtgatt ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag
    12121 tttttgatca tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc
    12181 gaggattgcg ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg
    12241 acctatttga agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa
    12301 atggccaaac aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa
    12361 gtagcgactt tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg
    12421 atatattctc aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg
    12481 caaaaaggcc ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa
    12541 gaaagatagt gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct
    12601 ttttcaaacc tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga
    12661 ggaatagcta acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga
    12721 ggaaaatcag tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata
    12781 ctttctgtac ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa
    12841 gtaagcgatg actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc
    12901 ctttttcgat tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt
    12961 gataagcgag tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg
    13021 gtaagtctgt gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg
    13081 tattttctcc ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca
    13141 gcacagtatt cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa
    13201 taccgatcgt atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag
    13261 taaagggatc tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca
    13321 tgttttcttg gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat
    13381 gagagatgat ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta
    13441 gtatccctat tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct
    13501 ttggtttcat ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt
    13561 tgcgtaatcc gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg
    13621 cgtctagcaa agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca
    13681 tagattccct taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca
    13741 ggagaaatca actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg
    13801 tttgttaatg gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat
    13861 tttagacctg tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct
    13921 ttcgccgata cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg
    13981 tcactatctt gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg
    14041 tcaccatcta ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt
    14101 tgatcatttg tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct
    14161 gctttttcgt tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa
    14221 ggaatataat tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa
    14281 tttactgggt cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa
    14341 tcattatcaa atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg
    14401 ttttgcgttc cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga
    14461 attttttcac cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta
    14521 gacgtatcat ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa
    14581 ggcgtgattg tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa
    14641 ttatattttc caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg
    14701 gcaaagtcat tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt
    14761 tgatcagttg tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt
    14821 ggtgttcctt cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca
    14881 gtaccgattt ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga
    14941 tggatagtat ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc
    15001 tcatatacag ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct
    15061 tctttgatcg tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca
    15121 tttccatcag catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact
    15181 gcttgttttg ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg
    15241 acgttataaa ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt
    15301 tctttcccgc tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg
    15361 atgacttgag ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt
    15421 aatggtaaaa taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt
    15481 ttttcactcc tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac
    15541 caacaacgcc taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat
    15601 tttctttttt attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag
    15661 ttgaaatggt aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct
    15721 cttttaatcg ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg
    15781 aaatcaggtg aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct
    15841 ggaggtaatt tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg
    15901 gtttatttgt ctcgattttg acaaattgct tactccctgt atatacagag actttctttg
    15961 tactgacacc aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg
    16021 ctgtactgct tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca
    16081 acgaagggat cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt
    16141 ctgtcagctt ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt
    16201 catccgcact atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg
    16261 tatgcaagga ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata
    16321 ccttctcaaa aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag
    16381 ttgaaagttt ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct
    16441 tttctttgac gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt
    16501 cttgatcttt agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag
    16561 ttgttgcttc tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg
    16621 cttcttctac cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa
    16681 cttggaatgt gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt
    16741 catttggatg atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct
    16801 gaaccatttc tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca
    16861 agaagagaca catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt
    16921 tttcaaaaaa agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc
    16981 taaacggcca attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa
    17041 agaaatctgg ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac
    17101 ttgaattgac ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc
    17161 ttctggtgta aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa
    17221 ggtatctgtt tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct
    17281 gaattttgct ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa
    17341 cgcttttagt cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt
    17401 tcccgtgtaa atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt
    17461 atcatatccg gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc
    17521 aaaactggca agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt
    17581 tgcttctttt ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc
    17641 ttgcatcgca tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa
    17701 ctggatcgta tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga
    17761 agcgatcttg atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt
    17821 ttctgtcagc gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt
    17881 tccgtcgcca tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc
    17941 gccaataaga tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt
    18001 gaattgttta gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac
    18061 aggtaattct ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag
    18121 tgataatatc tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg
    18181 ttcccacgta tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca
    18241 agctgcagca tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc
    18301 agctagattg ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc
    18361 tttagcagat ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt
    18421 tgtcctgccg ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat
    18481 tctcacttgg taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt
    18541 gatggtattt ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact
    18601 cttgactgaa agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg
    18661 tccattgctt acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc
    18721 tgttgcatga tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg
    18781 tcgcatacga gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg
    18841 gatgccaaga ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt
    18901 tgcgataaac gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat
    18961 tcttgagatt agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta
    19021 ataattgctg cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc
    19081 atccgtcagc aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc
    19141 atctcttagt gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt
    19201 tacttgattt ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata
    19261 tccttcgctt gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc
    19321 taaagtatcg acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt
    19381 catacttcct gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac
    19441 atttccacga gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata
    19501 ctttcgtatc gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt
    19561 ccgatcatta ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt
    19621 attgccttga tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata
    19681 agtgccctga tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac
    19741 agctggcaag gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga
    19801 tgattcttct tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc
    19861 tgtctcatca ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg
    19921 gtaaagtgct ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat
    19981 ttccaacgta attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc
    20041 cacatcactt aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt
    20101 gttattcgca tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc
    20161 agctgaaatt gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg
    20221 cacaaagttt gataaactca atattatcag catgaaaata actttcagtt tcttccctgt
    20281 ggtcgtcatt tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta
    20341 ttaaaataaa caggcgttac ataattttat ttattttaat atgataatta ttatacgaag
    20401 ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc
    20461 cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag
    20521 gttgttggaa tgaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga
    20581 caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt
    20641 aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca
    20701 aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga
    20761 aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa
    20821 aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt
    20881 gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt
    20941 tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt
    21001 aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat
    21061 tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc
    21121 tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga
    21181 atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat
    21241 cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt
    21301 tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat
    21361 acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga
    21421 atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac
    21481 gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg
    21541 tctatatgct ggatttcgtg cagaacttca attttattcc actctactga atcagaatct
    21601 gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca
    21661 ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata
    21721 tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc
    21781 tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa
    21841 tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc
    21901 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
    21961 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
    22021 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
    22081 tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac
    22141 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
    22201 tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
    22261 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
    22321 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
    22381 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
    22441 aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg
    22501 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
    22561 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
    22621 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
    22681 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
    22741 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
    22801 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
    22861 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
    22921 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
    22981 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
    23041 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag
    23101 cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
    23161 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    23221 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    23281 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    23341 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    23401 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
    23461 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    23521 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    23581 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    23641 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
    23701 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    23761 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    23821 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    23881 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    23941 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    24001 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    24061 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    24121 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    24181 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    24241 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    24301 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    24361 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    24421 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
    24481 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
    24541 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
    24601 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
    24661 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
    24721 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
    24781 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
    24841 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
    24901 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
    24961 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
    25021 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
    25081 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
    25141 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
    25201 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
    25261 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
    25321 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
    25381 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
    25441 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
    25501 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
    25561 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
    25621 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
    25681 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
    25741 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
    25801 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
    25861 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
    25921 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
    25981 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
    26041 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
    26101 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
    26161 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
    26221 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
    26281 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
    26341 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
    26401 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
    26461 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
    26521 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
    26581 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
    26641 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
    26701 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
    26761 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
    26821 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
    26881 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
    26941 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
    27001 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
    27061 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
    27121 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
    27181 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca
    27241 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
    27301 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
    27361 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
    27421 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
    27481 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
    27541 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
    27601 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
    27661 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
    27721 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
    27781 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
    27841 tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa atatttagat
    27901 tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat
    27961 attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc
    28021 cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa
    28081 aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga
    28141 agtaccaact atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc
    28201 attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa
    28261 taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta
    28321 accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata
    28381 aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt
    28441 tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag
    28501 aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag
    28561 aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa
    28621 atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta
    28681 ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag
    28741 aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa
    28801 ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga
    28861 agcaatattc attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag
    28921 agatttctct tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg
    28981 atgcagaact tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa
    29041 gcagagaagc tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc
    29101 ttctgtattg cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt
    29161 tgaccaagca gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac
    29221 caaaagaagc gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg
    29281 aaaattcttt tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc
    29341 aaattttgag caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat
    29401 atcgttatta tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta
    29461 atcgatacct ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt
    29521 atcacgaatt agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt
    29581 tttcacaata aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat
    29641 attacttaac aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga
    29701 acttatttac gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg
    29761 atgagccact ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg
    29821 aaagtgtgtt gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag
    29881 ttattattta taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc
    29941 ttggtgcagt gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta
    30001 tcgatcgttt agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag
    30061 aaatttcaaa tggaacaaaa tcaaaacaga ttcc
//
LOCUS       DACDCV010000025        29292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDCV010000025 DACDCV010000000
VERSION     DACDCV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.025"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..898)
                     /locus_tag="IV041_001110"
     CDS             complement(50..898)
                     /locus_tag="IV041_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8381919.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1122..3062)
                     /gene="thrS"
                     /locus_tag="IV041_001111"
     CDS             complement(1122..3062)
                     /gene="thrS"
                     /locus_tag="IV041_001111"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8381920.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3399..3578)
                     /locus_tag="IV041_001112"
     CDS             complement(3399..3578)
                     /locus_tag="IV041_001112"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8381921.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3625..5448)
                     /gene="cadA"
                     /locus_tag="IV041_001113"
     CDS             complement(3625..5448)
                     /gene="cadA"
                     /locus_tag="IV041_001113"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8381922.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5726..8005
                     /locus_tag="IV041_001114"
     CDS             5726..8005
                     /locus_tag="IV041_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8381923.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8098..8745)
                     /locus_tag="IV041_001115"
     CDS             complement(8098..8745)
                     /locus_tag="IV041_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381924.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8924..9643)
                     /locus_tag="IV041_001116"
     CDS             complement(8924..9643)
                     /locus_tag="IV041_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8381925.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9774..10925)
                     /locus_tag="IV041_001117"
     CDS             complement(9774..10925)
                     /locus_tag="IV041_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8381926.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11142..11882)
                     /locus_tag="IV041_001118"
     CDS             complement(11142..11882)
                     /locus_tag="IV041_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8381927.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11886..12209)
                     /gene="rsfS"
                     /locus_tag="IV041_001119"
     CDS             complement(11886..12209)
                     /gene="rsfS"
                     /locus_tag="IV041_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8381928.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12266..12859)
                     /locus_tag="IV041_001120"
     CDS             complement(12266..12859)
                     /locus_tag="IV041_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8381929.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12843..13490)
                     /locus_tag="IV041_001121"
     CDS             complement(12843..13490)
                     /locus_tag="IV041_001121"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8381930.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13503..13814)
                     /gene="yhbY"
                     /locus_tag="IV041_001122"
     CDS             complement(13503..13814)
                     /gene="yhbY"
                     /locus_tag="IV041_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8381931.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13830..14939)
                     /gene="yqeH"
                     /locus_tag="IV041_001123"
     CDS             complement(13830..14939)
                     /gene="yqeH"
                     /locus_tag="IV041_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8381932.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14936..15463)
                     /locus_tag="IV041_001124"
     CDS             complement(14936..15463)
                     /locus_tag="IV041_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8381933.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15643..16428)
                     /locus_tag="IV041_001125"
     CDS             complement(15643..16428)
                     /locus_tag="IV041_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8381934.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16421..17290)
                     /locus_tag="IV041_001126"
     CDS             complement(16421..17290)
                     /locus_tag="IV041_001126"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8381935.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17291..18670)
                     /locus_tag="IV041_001127"
     CDS             complement(17291..18670)
                     /locus_tag="IV041_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8381936.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18678..19097)
                     /gene="fabZ"
                     /locus_tag="IV041_001128"
     CDS             complement(18678..19097)
                     /gene="fabZ"
                     /locus_tag="IV041_001128"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8381937.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19122..19598)
                     /gene="accB"
                     /locus_tag="IV041_001129"
     CDS             complement(19122..19598)
                     /gene="accB"
                     /locus_tag="IV041_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8381938.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19601..20836)
                     /gene="fabF"
                     /locus_tag="IV041_001130"
     CDS             complement(19601..20836)
                     /gene="fabF"
                     /locus_tag="IV041_001130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8381939.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20855..21592)
                     /gene="fabG"
                     /locus_tag="IV041_001131"
     CDS             complement(20855..21592)
                     /gene="fabG"
                     /locus_tag="IV041_001131"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8381940.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21604..22521)
                     /gene="fabD"
                     /locus_tag="IV041_001132"
     CDS             complement(21604..22521)
                     /gene="fabD"
                     /locus_tag="IV041_001132"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8381941.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22628..22852)
                     /locus_tag="IV041_001133"
     CDS             complement(22628..22852)
                     /locus_tag="IV041_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8381942.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22905..23867)
                     /locus_tag="IV041_001134"
     CDS             complement(22905..23867)
                     /locus_tag="IV041_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8381943.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23877..24317)
                     /locus_tag="IV041_001135"
     CDS             complement(23877..24317)
                     /locus_tag="IV041_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8381944.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24623..25588
                     /locus_tag="IV041_001136"
     CDS             24623..25588
                     /locus_tag="IV041_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8381945.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25645..25875)
                     /locus_tag="IV041_001137"
     CDS             complement(25645..25875)
                     /locus_tag="IV041_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8381946.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25961..26848)
                     /locus_tag="IV041_001138"
     CDS             complement(25961..26848)
                     /locus_tag="IV041_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8381947.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26937..28160)
                     /locus_tag="IV041_001139"
     CDS             complement(26937..28160)
                     /locus_tag="IV041_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8381948.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28177..29256)
                     /locus_tag="IV041_001140"
     CDS             complement(28177..29256)
                     /locus_tag="IV041_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8381949.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8117 a         6418 c         5049 g         9708 t
ORIGIN      
        1 aactacgttt tgttattcta aaaaacttat ataaaaaata gtaaggtttt tatttcgaat
       61 ccataaaata ttcttggatt gcttctcgtg tttttttcac tttttcgcta agcttttcgt
      121 ttttagccat atagaaataa aaaatgatat cgatcgtagc aaattgggcc aaaagtgaat
      181 tagttgccgc acttcgattc gtgatttctt ttggtctaga tgtttgtacg agtacatcgg
      241 ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg atattcatgc
      301 ttttcgccag ctcggctaat gcgactacat ctgcacttct acctgagttt gaaacaagcc
      361 aaagcacttt ctttttctca ttgctgacaa gttgcgggag caagacgtgg atatcttttt
      421 caaaaatgat tggcttaccg acacgagacc atttttgcag aatatcttct actactaaag
      481 aagaggcgcc aaccccatat aaaaaaacag tctctgccga ttccaaacac tctacgactt
      541 tttggatcgt ttcttcttct agaatatcta ctgtttcttt tatcgctact tgtgcattgt
      601 taccaagttt gtttttgata gagtaaaagg actcatttgc ttctatatca ctaaaacctt
      661 catcgattcc agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc agactttgaa
      721 aaccgatttt tttggaaaaa cgtacaactg tcggcgcact caacccggaa gcttctgcta
      781 attcattagc tgtcattgta gggaccttct caatattacc gataatgtag ttggccaact
      841 ttttttctgc tgaagaataa tcttccatca tactttcaat acgactaatt acgctcataa
      901 gttcctccac ccttttaatg atagtctatg taagtatacc atatcagacc cttattcacg
      961 gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa cagaatatct tatcccatca
     1021 atcccacaga ttctattgag catctgtgtc tttgaggaat acctgttatc tattgttcgg
     1081 attgctttct atctactact tttttacagg aaatttacct attagttttc tctgctgtaa
     1141 tttttgactt ctgcttccat agcatcaata aagatattca aatcttctac agctgtttct
     1201 ttacttccat aacgacgtac gttcactgtt gcatcttcca attccttgtc tccaacaact
     1261 aattggtaag ggatcttttg tgtttgagag gcacgtatct tatagcccat tttttcatta
     1321 cgatcatcaa cttctacacg caaccccttc atttgcaaac gttctttgat ttcataagca
     1381 taatcgccat gtgcatcaac agaaacaggg atgattgttg cttgtatagg tgccaaccat
     1441 gttggaaaag cgcctttgta tacttctgtt aaataagcga caaaacgttc cattgtagaa
     1501 acaattccgc ggtggataac tactggacga tgcgtatttt ccccgtcttc tccaacatat
     1561 gtcaaatcaa agcgctctgg caataagaag tctaattgta tagttgataa tgtttcttct
     1621 gtaccaagag ctgttttgac ttgaacatct agttttggtc cgtaaaaggc tgcctcgcct
     1681 tctgcttcaa agtaatctag ttctaattca tccatagcgg ctttcagcat tgcttgggct
     1741 ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc gcgatagctc
     1801 aagcggaaac gataatctgt aatattgaag tcagcatata ctgccaccat caattcaagc
     1861 gtacgtttaa attcatcttt gatttgatca ggacgaacaa atgtatgacc atcattcaac
     1921 gtcatttctc gtacacgttg taaacctgac aaagcacctg atttttcata gcgatgcatc
     1981 attcctaatt cagcgatacg aattggtaat tcacgataag aatggatcgt gtttttgtag
     2041 accatcatgt gatgagggca gttcattggg cgaagaacaa gcatttcgcc atctcccata
     2101 tccattggcg ggaacatgtc ttcttgataa tgatcccagt gtccagatgt tttgtacaac
     2161 tcaacatctc ccattattgg tgtatataca tgttggtaac ctagactaac ttctttgtct
     2221 acgatataac gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca gaatggcaaa
     2281 ccagaaccta cttctgggga aaccatgaac aagtcaagtt cttttcctag tttacgatgg
     2341 tctctttctt ttgcttcttc acggagacgg atatattctt tcaaagcttt cttgtcaaaa
     2401 aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg agtttccacg ccaataagca
     2461 cctgccactg ataataattt aaatacttgt atacggccag tggatggaac atgtggtcca
     2521 cgacacaaat cgatgaattc gccttgttga taagcagtaa tcacttcttc ttctggcaat
     2581 tcactaatca gttctacttt gtacggatca cttgcaaaaa tttctaatgc ttcctgtttt
     2641 gaaagaacac ggcgttcgat cggcagattt tctttgacga ttttcatcat ttctgcttcg
     2701 attgccggca aatcttctgc agttacttgg ttttgtccat tatctgtatc gtagtaaaac
     2761 cctgaatcga tagctggtcc aacgccaaag tggatatttg gatagaggcg acgcatggct
     2821 tgggccatca aatgagctgc tgaatgacgg actaagccaa gtgcatcttc atggtctggt
     2881 gtgatgatct caatacttgc atcttcctcg attggacgag tcaaatcaac gagttttccg
     2941 ttcactttac cagctaaagc tttttttgct aagctttttg aaatgctttc agcaattgcc
     3001 aatgttgttg ttccagactc aaattctttg actgcaccat ctggaaaagt aatttttatc
     3061 ataatagttt cctccttgaa atgttttgat tgatcagatc atcaagctga aaaacaaaaa
     3121 aatcctagca tctcttgaaa agagatacta ggacgaaaag ttcgtggttc cacctaatgt
     3181 tcaaacgcaa atgcgttcct caagcgttgt aacggtccga ccgcccttgc ttcaacaagg
     3241 gtttcgaaag tggtcatcct ttggttttct cctggaaaag tttcagccta gcttttcctc
     3301 tcttatcgaa gactctcaaa ttcagttggc tttctcatca atcaattatg tgtttattta
     3361 accactaata aatagaaaaa tcaagtggga aactaaactt attttttcca ttccccattt
     3421 acaccgtatg ttcctttttt catctcattg atgatgacat ggatgtgttc ttttggtgcc
     3481 tgcgtgtttt tatgaacagc ttccgtgata tctttcatca tattttctaa ttgttccgga
     3541 ctgcgacctt ctactaattc gacatgtacg aatggcataa tgctctctcc tctttctgat
     3601 cgttcttttt tcaactcgat tctatcatac tatgtttgtc ttgggtactg aatcaggaaa
     3661 aatggctgga tctttttttg ctctcaaggc actcaaaata gagaccgtat ccacgacttc
     3721 ttgcaaaaca gctccgaata atgcggggat tagtcccgta cttgcaatta acatcaaaaa
     3781 aatacaaata aagatgccaa tcagtacaga ctgtttcgct attctcatcg tatctcttga
     3841 aatcactact gcttttgcta cacgcaagag atcatctttc aagatgaccg catctgcact
     3901 ttcactagca gctgtagaac catgagcccc cattgcaata ccgatatctg cagcagcaag
     3961 tgctggtgcg tcattcacgc cgtctcctac catcgcaaca ggtctttgat ctttagggat
     4021 actttctaaa atggtcagtt tatcttgagg caagctacgg gcatgtacag aatctaattg
     4081 gactttttct gcaatcgttt gagcaatggc agatccatcc ccggtgatca tcatcaaatt
     4141 tttcacatca agttttctta gctccttgat tgtctttgct gcttctgggc gaatctgatc
     4201 cgaaaatagg atcgttcctg caaaagatcg atcaattgtt acatagattg ctgtattgga
     4261 cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca atctttactt catgattatt
     4321 gactactgct tggattcctt gaccggtcac ttctttcagt tgactaacag gatacagttg
     4381 gctgtttttc acgttgttca ctaacgaacg agctaagata tggctagatt cctgttctgc
     4441 actagctgca tacagaagta gctcttcttt ttttattcca ttgactggct gaatcgcctc
     4501 tacttccaat atccctttgg taagcgttcc tgttttatca aaagcaatcg tttttgtacg
     4561 tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc ccgtttttac ttgaacggct
     4621 cattcctgca actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg atgccactac
     4681 cagtacctca gcaaatcgta aagggtcttt tgagaaccac caagaaatac caccgatcaa
     4741 ataagcaatc aacgtgaaag ggactgcata acgatcagct aaacggacaa atcgggcagg
     4801 cttttctttt gattcttcga ctagtttaat aatcatttga tactggctgt ctgcggcacg
     4861 tttttcagcg atcatttgga tcgatccgtc tccatttaaa gaccctgaca taatctgatc
     4921 tccaggtttc ttggtttccg ggcgggactc acctgtcaaa gaagattcat cgaccgaaga
     4981 ttctcctttg gtcactcgac catctgcggg tacaagttcg ccaggcttga ctagtaactg
     5041 gtcatcaata tttatttcat cgattactat atcctgcaat tttccttctt tgaatcgatg
     5101 ggcaattctt ggggtgttat ctaacaaaga ctgcagttct ttacccgctc gtttattcgc
     5161 gtaatcctct agactatctc cgcccgtaag catgatcaat atcatcaaac tagcccagta
     5221 ttctttgacg gctaatgttg caaggattgc ggtaatcgct aatacatcta cgccatatct
     5281 tcctgaacgt aacgtcttga tcatttccaa aaacatcaag aaggcgagaa tccctccagt
     5341 aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa acaaattcac ttaataaagc
     5401 tatcccacca ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg ttttttctcc
     5461 tcttttatgt gacttagaga ggttagtaat aaatttccag ctgcctcttt catcttaagc
     5521 atagtccttg actagcactt tttaaaacaa taggccttaa ttgaaaacaa ctatcactat
     5581 cagcgtttcc aaaattcatc agttctacaa acaaaaatat tcagcaagga ttactttttc
     5641 gattcatcag ttaaaaagta ccttttttcg aaaaactatg ctatactttt aattcgttat
     5701 ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa cgcacgtgga
     5761 tgaaacgatc gaactaatcc agatcgaaca aaatatgctg gatacaaaac aacaagaatt
     5821 gactcaggaa atgaatcatt cgacaaaaga cacagctaat cacaagattc gagctggttc
     5881 caatgaagct ttttatgaat ctgctgttga atatcgccaa catgagcagg aattgctttt
     5941 gaaataccat actcttgaag cacagcaaaa acgcctgcaa acgttgtctg tcatgaaaga
     6001 tagtccttat tttgcccgta tcgactttaa agaagaccgt gaaacagaaa cattatatct
     6061 aggtatcgct tctttaagag atctggcaga agaaacaatc gttattgact ggcgcgcacc
     6121 aattgccaat ctttattatg aaggtgagat tggtcccgca ttttatgaaa ctgaaacaga
     6181 tagaatagat gtcgatctgc agttgaaacg acaatttaaa atccaagaag gacgtatttt
     6241 atctatggta gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga tattggatga
     6301 atcatcaagc aaccatatga aaaacatcgt ctctacgata caaaaagcac aaaatgcaat
     6361 cattcgggac acaacaagta aagtaatgct gattgaagga atcgccggaa gtggaaagac
     6421 atccgctttg cttcaacgta ttgcttttct gttgtatcat aatcgcaagt ggctcgaggc
     6481 tgaaaacgtt cttctatttt cacccaacca tttgttttct gattacatct cgactgtatt
     6541 gccttcatta ggtgaaagcg gcgtgcctac acagacattc aaaaattatt tggtgcagct
     6601 gctgccagag ttttctttac tagatgaaca gcaacaagaa agtggttttt tgctagggga
     6661 aaaagatccg atacaagtga tgaaaagcgg attgacctta gtcgatcaga ttgatcgcta
     6721 tatccaaggc atcacaaact acggtccctt gttccgtgat atgaaaataa atggccgtac
     6781 gatcctatca aaggaatcga ttcgccaatg gtataaagaa acaaatgaac aattaccttt
     6841 acatcataga ctttctctat tacaaacgaa gctcctgaaa aaattaggcg gtctgcaaaa
     6901 agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc aagaactata
     6961 cgcaacagat ccaaatcttg aatatacaga acaaaaggag cgttcattac gtaaaaaact
     7021 ggccaaccaa atcgtacgta aaagattccg tcgaatccat cgagggatca tgaattatca
     7081 atttgtcaat ataacgaaac aatatctcca ctttctacaa actgtgccac tttctcttct
     7141 aacacgacat aaaatcagcg agaccaggtg gcaggaacat ttgacaactg ttcgtacaca
     7201 cttgcgtcaa agagaattgc gtcaagagga tgctgtacta ttctttttac tgatgcgccg
     7261 aacatatcca gtgacggtta cacacaaagc tcgctatatc tttattgatg aaatgcagga
     7321 tttcgcacca tcacaagtgg cattgctgca agccttgttc ccaaaagcaa atttgacttt
     7381 ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat cattggaaga
     7441 ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg acaagctatc gctccacaaa
     7501 agaaatcact gattttgcta atcattttat tgtgaaagaa catcaagtag aaacaacagc
     7561 aagaaaagga gaccttcctt tgctcatcca aggaacaaca gatgatatca aattgaattg
     7621 gttagcagat accattcgaa cgactcaggg aaaagcccgg tattggcgta cagcaatcat
     7681 agctaaaaca caagaagaat gcgaagctct ttatgctcaa ctgccagacg agttgaaaca
     7741 acaagtccag ctgattttaa atgagagtga ctttatgaag cgttccatta ttttacttcc
     7801 agccttttta gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg tagatgcaga
     7861 acatttttct tctcagcaag acaaattgat cctttacacc atgtgtacac gtgcgatgca
     7921 tgaattgacc ctttttacct ccaataatga aagtcctttg ttaaacaata tggatatttc
     7981 atcttatgag aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa tgttagcagt
     8041 tgtcttttta tttacaataa taattgcaga aaaagtaatt caatcattta aggtcattta
     8101 ttctatatat aaatccaaat ctgtttcagg atattttaat ttgacaagcg aaataatcat
     8161 tttcagttct tccatgtttt ctggatattc tgggtcaaat accttgctat gacggccaat
     8221 cccgccattg tcgatcaatc ggtaaattcg attccattca atgaattggt caattgattg
     8281 gaaattgctg attggatagc cgccacaatc caaaatctct gatacacgaa atgctaggta
     8341 tgcactatcg atcactttat tttttttcgt gtctatcttc tcatttcttt cgataagaat
     8401 tttttcagga cgatgtttca agctccactc taatagtaga aagaaagcct ccatccgata
     8461 ctcttcttca aataaccgaa aggctgaatc agaacgacga aaaagcagcg gaatcagata
     8521 attttgtcta taaatcagca ttggtgcgcg gtccaccata cactctaaat aacggttcag
     8581 ataccctgta ccgacatagt ttcctaatcg atagccttca acgccgaatc ttgcgtattt
     8641 ttgcgctaat tttgaacaat cagttacgtt cacagctaac tcttctactt tgcggtaacc
     8701 gcttaagata cgttcaaaag gttctaaagt gatttttgct tccatattct tccccgtcca
     8761 tcttccacat acttcatatc atttcacatg atacttatgc tctcatcata ccacaggttg
     8821 aattagaaaa gaaaataaat cacacaaaaa agcaagacaa tctgttagtg agattgtctt
     8881 gcttttcgaa aaagactgaa tacttttcgt taaaactggt tgcttagtct tctaatgcca
     8941 cattatggaa cacatgctgt acatcttcta gatcttccag tgcatcaatc attttttcaa
     9001 atgtttcttg atcttctgga gatagtgtta cctcgttttg agggatcatt tggatctcag
     9061 cgattgaaaa atcagtgatt cctttttctt ttaatgcttc ttgtatacta tggaaatccg
     9121 ctacttcacc gtaaacgatg atttgtcctt cttcttccat tacatcgcga acatcgatgt
     9181 ctttttccat taggtattca aggatctcgt cagcgtcttc tcctgcaaaa gcaaagattg
     9241 ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc catgttcccg ccatttttcc
     9301 caaatgctgc ccgtacgtct gctgctgttc gattcacatt gttcgtcaat gtatcaacaa
     9361 tcaccattga gccgcttggt ccaaatcctt catagcgcaa ttcagagtat gtttcatctc
     9421 ctgcaccttt tgctttttcg attgctcggt caataatatg ttttggtaca ttataagttt
     9481 tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg cggatcagga tcacctgatt
     9541 ttgctgccgc gtagatttca atcccaaatt ttgcataaat ccggctatta ttagcatctt
     9601 tagcagtttt ctttgctacg atatttgccc atttacgtcc catattttca cttcgctttc
     9661 tgatttgata gtttcataac gcaaaaacgt cctgttccat tatacttgtg aatcacgatt
     9721 ctgtgaagct ttttttcttt cttccttcat tcttctcatt tttagtcaat tatctatact
     9781 cggattggga tttgcccgaa attctgttcc ggaatattgc ctcccaattg atagatttga
     9841 tcacttctaa ctaacagttc cgcttcttgt gcctcttttt ttccgattcg ggaataaata
     9901 gaaatctctt ttttttcttt taagaatctt cttccagcat cggtaaatcc aagtagtcga
     9961 agataatttt tattttgttg gacaggaatc tcttgctttt tgatattcag caagacataa
    10021 caagataatc gttggatccg tgtccatgta tatctttttg tttttacctt tgacacaaaa
    10081 tcctcaaaac tatcagcagt tgcagctgct gttttcatgc gcgattctat tccttcggtc
    10141 atctgataga tatctgtcag ttccagcaaa gtagaagata acaaccgata acgcaaatac
    10201 ggccaataag attcccaagt ctgcagcgaa caagttgaaa gatcttcttc tgtctgaggc
    10261 ggcacaagtc cagctattct ttttccttct tctaacgcat ggcgaatggc tgttgcactg
    10321 gctatcctct gatctgtgat gattttttca tgatagcctg cacttttacg tcggatcgga
    10381 tgtaattcca tagggtggac atacttggca ttttcctttg cataacttaa tcccaagata
    10441 tgatttggcg aagaaaaatc aagcgtaata tcgggataca gactgcgaaa aacctcagtc
    10501 attttttgcg gataactata cgtttgatca gacagttctt gaaatgcctc ttctatccta
    10561 tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat caaacgaagt ctcttcttct
    10621 gttccaaaac acaaactgtc acagtccagt gtttgcaaga tttttactgc acctttagca
    10681 aaatagtcag ctgactgaac cgcccattcg acgggaagct ccactactaa atccacacca
    10741 ttttgtaaag cttcattcgc gcgatgccat ttatcgatga tagccggttc gcctctttgc
    10801 aaaaaattcc cgctcattac agcaatgacg acctctgctc ctgttatttt tcgtgccatt
    10861 tccacatgat agcgatgtcc attgtgaaaa ggattgtact ctacgataat cccgcagctt
    10921 ttcatgcgca tcctccttca tagttatcga taaaaaagaa atagagacaa gtaaaaaaag
    10981 gcaaaaatcg gaaaaaatac ccgaactata taaactattc tcgcaaacac aatacttctg
    11041 cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt cggatatata atttatgttg
    11101 aagatttatt cttttcttgt taactcgagc aggcaaaacc gctagtcttt gtatgctaca
    11161 aagaaccagc gctttgattt ctctgatgct gcttcgtctg taaaatcaga aaatgcttcg
    11221 actttcgaaa agccgctgtt ttccagcatt cttaaataac tttccaatgg ataggtacgc
    11281 tcttgatgta gttcatcttc acgaataaat cgaccagacc cttcttgttc ttcaacgaaa
    11341 aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg gataactatc ccacaaaaaa
    11401 gcaaaattat ccccttgata atgatagcta tattctggaa aaacctcatc tacttgatag
    11461 gtggagtgta cgtcaaataa aaatcggcca ttttcttcta acgcttgata gacttcatca
    11521 aacacttgct gtacctcttg tctatttttc atatagcaca gcgaatcaga aaaacaggta
    11581 attgcctgat attgtccgat ttctgataaa tcgagcatat tgccttccac aaattggatc
    11641 gatgcgtctt cttcatatgc tcgctggctg gcaatcatca acatctcttc tgaaaggtct
    11701 aaccctgtca ctttatatcc atcttttgca aaggcaaccg ctaatgctcc agtaccgcat
    11761 gccagttcca aaatattttt tgtgtcttct tgtaaatgac gtttcgaaaa agctaaccat
    11821 tgctgataaa gtgtttcgtc catcacttca tcgtaaatga aagcaaatgt ttcataagcc
    11881 atatgttaat ccaaccattc actaagatcg actagcggag catctgacca tagtttttcc
    11941 agattataga aatttcgttc tggcgcatgg aaaacatgta cgatcacatc tccaagatcg
    12001 atcaataccc attttccgcc ttcttttcct tcaatacgtt ttacttcata tttgttttct
    12061 tcttctttat caatgatttc ttctgtaatt gcgttgattt gacgttcgct atttgctgaa
    12121 cagatcatga aataatctgc caacaaggaa acatttcgaa cgtctaaagc gataatgtct
    12181 tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta attgttcttt actatctatg
    12241 atgatttcct cctaatatga gcagcttatt tttttgctac ccagtaatta tatgtttcga
    12301 ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg ggccaatgta tgttttgttt
    12361 caaatgctac agcttcgtct agatcagccc atgcaatggc tcgtgcatct tgtaccccag
    12421 gaaaatctcg acctggttca atatagtctg ctacataaat gattttgtca aggagactca
    12481 tcttagcagc cccagtcgta tgcacccgga ttgcatgaag aatctctgca tcggtgattc
    12541 ctaattcccg ctctacaaac gaagcgccaa caagaccatg ccagattgca ttattataat
    12601 tcaataactc cggatcaaaa ccgtctctta cgattaccat tttgaactcc tcatctggtc
    12661 gttcttttgc ataatcatgt gtcaatgcag caatactagc tttttcagga gaagcaccgt
    12721 attttttggc aagagccacg gctgtttctt ctactccaag cacatgcttg aagcgttgtt
    12781 cgctcattct catctgtatt ttttgcatca ggttttctct agaataagca gtatattcat
    12841 tactatagtc catattcata caacccttca ttttgaatat aatcaattac tttatctgga
    12901 acaaggtagc ggatagaaca gccttctttg atcttttgac gtattttcgt ggaactgatg
    12961 tcgatctcag gtacatctac ccaaattaca ggatatggag tatccgttgt gtatcctggt
    13021 cgacgtatac ccacaaagtt gaccatagaa gtcagctcat cgattttata ccattttggc
    13081 aaatattcta ccatatcccc accgataata aagtaataat ctgtatccgg attgttttgc
    13141 gtcaattcct tcatagtatc ataggtgtaa cttttcccac cacgagctaa ttcaatcgtt
    13201 tcaatctgca aaaaaggatt gtcttccacc gctaattcca acatgttgag tcgatgtttt
    13261 gcatcaatcg tctgtttctc atcaacatga ggtggctgat aagaaggcat caaaaatacg
    13321 cggtcaagac ctaaattccg gccagcttgt tcagccatca ctaaatgtgc aaggtgaacg
    13381 ggattgaatg ttccgcccaa gatccctact tgctttttct tctctaagaa ttgaggcatt
    13441 tcttcaggaa ataattgtga acgaacaaat gtcttagctt gcgtattcat catggtcact
    13501 ccttatattg cttttacttc ttttgatagc ctttgatact tttcttttgt ggaaggtttg
    13561 tataatacaa taacacgtcc aatgatttgg acgatttcgc aatctatttc tttttctaat
    13621 acttgagctg cttcttctgc aatctcatct gtattttgaa gtaacgatac tttgatcagt
    13681 tctctttttt ctaaagcttc ttcaatttga acgatcattg ctgaattgat cccacctttt
    13741 ccgatttgaa aaatcggctg aagatgatgc gctttgctac gtaaaaaacg cttttgtttt
    13801 cctcttaaac tcataatttt ccttcttcct taaatcaatg ctttgcgttt caatacatct
    13861 acaccctttg gcgcccagcc cgcaattact actggcgctg ttacagtgat ccagcctaga
    13921 ccggcaaaaa cgatgtctgt tttttctttc acagaaaact caaaacgaac aagctccgga
    13981 aatgcctcga cttcatcagg acgaggaggc tgtaataatc cacctacatg tttttgataa
    14041 aattcgtctg ctttttccag tttcgttcga tgaatattta catcatttga cacataagca
    14101 ataaatgaag cacgattgcc gctgataaaa tcaaatcttg caagtccgcc caagaaaagt
    14161 gtttgccctt cattcagctg ataaacttta ggtttgattt cttttaacgg tgcaatgatc
    14221 cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc gatggataat tcccggtgta
    14281 tcaatcaaaa aatgcccgtc atctaaagga atctcgattt tatccaaggt cgttcctgga
    14341 aactgcgaag ttgtgattac atcttgtact cccgctgtat ttttgatgat ttgattgata
    14401 agcgtcgatt ttccgacatt tgttactccg acgatataga catcccgtcc atcgcggtat
    14461 tcttcgattt tttctaatag ttccgtcatc tcatgagatt tcttagcact agttaataat
    14521 acatctactg gacgaattcc tgcttcgtaa gcacgttcct tcatccattg tgttaattta
    14581 gtttttttca atgatttagg taatatatcc actttgttcc caactaaaag tactggattg
    14641 tcaccgacaa aacggtgtag tcctgggatt agtgaaccat tgaagtcaaa aatatccacc
    14701 acattgacga tcaatgcatc tttttgccct aattcattca gtaagcgcaa aaagtcgtca
    14761 tccgtcaatg agacatcttg ggtctcatta taatggcgta atctgaaaca acgctgacaa
    14821 tagacttccc cggaagctaa acctttttcc aaagccgttt gcggcgtgta tcctaattca
    14881 ccgggtttgt ttgtttgtat aacggcacca cagccgatac attgtaattc ttctgtcatt
    14941 cgattccacc tcgccagccc atatcagggt gttttgctaa tagataggcc ataattcttt
    15001 tttcccaaaa acgattaaat tgtgtcttcc aactatctgt tgcgataatg ggttttacaa
    15061 ggatgctacg tatccctgca cgattggctc cacgtatatc agtcataatc tgatcgccta
    15121 ccattactat ctcatcatct gctaaatcca attgtttctt tgctatgttg atccctcttg
    15181 ctaaaggttt cattgctcgt gcaacataca aaagatcaaa tttttcaatc gctcgcgcga
    15241 cacgttttga attattgttt gaaacaacaa caacagggat tcctgcattt ttcatttcta
    15301 aaatccaagt aagcaactct tctgttccat ctggattatt ccatgcaatc aacgtattgt
    15361 ctaaatcggt caatactgct ttgatgccta gattctttaa ttgggcaggt gtaatctgat
    15421 agatggcttc taccatccat gttggtttgt aatttgaaaa catgctgtct cctcatctgc
    15481 aaaaaatgaa ggagcacata aagtactcct caaccccgta ttatactgca tatcagcaaa
    15541 aaaaactacc tctatactcg ttcgtctttc agaaaaagca tttctttaca aaatgaccaa
    15601 aaataaagat tgatcacaga aaaatttaga aacaaaaaac aactaatatt ggcggaacct
    15661 cttgtatcgt ttttccagta atttatgctg atctaatcga acaagttccg tcaatctttc
    15721 aatcaacgct tttttcataa tgtgtgcgac tttatatcgt ggcaactgct cgccgttgaa
    15781 cgtttcagga ataactcgat caacgattcc taactcgcgt agttctggcg cagtaatttt
    15841 catcaactca gctgcttctg cggcgcgact accgtctttc cacaaaatcg acgcaaatcc
    15901 ttctggagaa agcacagcat aaatcgtttc ttccagcatc cagacttcat ccgctaaacc
    15961 tagagcaagg gcaccgccac ttccaccttc accaatgatg atggcgatga tcggtacctt
    16021 taaatcagac atttcgagca gatttctggc aatagcctct ccttcgccgc gctcttctgc
    16081 tcctacaccg caatatgcac ctgctgtatt gatcaaggta atcaccggtc tattgaactt
    16141 ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg gagaaccaaa
    16201 atttctgcgg atattttcct gcagatcccg tcctttttga ataccgatca ctgttaccgg
    16261 tttgccatct aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa ataaacgatc
    16321 accatggcat tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc ttgtccatcg
    16381 ttctttagct cttgctaact gaacgacatc atgtgctgtc ttatccacga tctatccatc
    16441 ctttctgtgt atgcaattca agaagcgtat ggattttctg tttcaattcc atgcggggaa
    16501 caatctgatc gacaaaacca tgagataata agaattcagc cttttggaaa tcttctggaa
    16561 gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc aagctttggg
    16621 gttctgctag aataatatcg ccttccattg caaaactggc agttactcct ccggtcgtcg
    16681 ggtccgttaa aacagttaga taaaaaagac cttcattgct atgccgtttg acagcagcag
    16741 agatttttgc catctgcatt agagaaaaga ttccttcttg catccgtgcg ccacctgaag
    16801 ctgtgaacaa gacgactgga agactttcct ttgttgctcg ttcgaataat ctggtgatct
    16861 tttctccgac aatcgttccc atacttccca tgataaaatt aggatccatc actccaataa
    16921 tgaagggaat cccctgaatg gttgctttac cagtgaggac tgcttcatga agtcctgttt
    16981 tatcttgcat ttttttaatt ttttccgtat attcgggaaa tgctagcgga tctttggtta
    17041 caagatccgt atcccattct tcaaaacttt tttcatcaat aatcaaagct agacgctgcc
    17101 atgcaccgat tcgaaaagcg tatccgcaat gagggcatac tttttcttca ccgatgtctt
    17161 tcgtataaag gatatgtttg caatttggac actttgccca catgttatca ggaacagatg
    17221 gcgagttagc aggtttgctc tctctattag gattgatccg aatatatttc tttttcttaa
    17281 acagagccat ctatgcgcct cctatctccg tttctggtgt ccaattcggt aaaaattctt
    17341 cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc aggatgacta atcaaatcca
    17401 tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag gctcgctgca
    17461 tcttcattaa tgcttcaaaa cgcgtatttc catggacgat tatttttgca atcattgaat
    17521 cataatatgg cgggatcgta tatcccgggt aaactgcact gtctactcgc agtcccatcc
    17581 cacctgctgg aagcagtaag ttttggatct tccctggaga aggagcaaaa tggaaagcag
    17641 gattttctgc attaatccgg cattcgattg cgtgtccttg gaatactact tcttcttgac
    17701 acacattcaa aggctcaccg gctgctatct ccacctgttt ttttactaaa tcgattcctg
    17761 tgaccatttc tgttactgga tgttccactt ggattcgagt attcatttcc atgaaataaa
    17821 aatcgccttc ttcatccatc agaaactcga tcgttccggc attttcataa ttgactgctt
    17881 tagctgctcg aacagcagcg tctcctagtg cttctctttt tgtttgtgag atcactacag
    17941 aaggagattc ttccagtact ttttgattgt tgcgttgtaa agaacaatct cgctcgccta
    18001 aatgaatgac atgaccaaat cgatcaccta agatctgaac ttctatatgg cgagcaggat
    18061 aaatgatttt ttccaaatac atatcatcat ttccaaatgc cgctgctgct tcttgctgag
    18121 ctgaagaaaa atgctgtggg agctcttctt ttgattgaac tttccgaatg cctttcccgc
    18181 caccacctgc agctgctttc agcatcactg gataaccaat tctgtctgct atttccaatg
    18241 cttcttctac tgtagataat acaccagtac ttcctggaat gactgggaca tttgcttctt
    18301 gcatcaattg acgcgcattg atcttatttc ccattgcatc gatcgttgca cttttaggac
    18361 caataaatgt gatattgcac tcttcacaca tggaggcaaa tcggctattt tcagataaaa
    18421 aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc agtacttcct
    18481 gaacattcag atatgaatca acggcttttg ccgggccaat gcagattgct tcatctgcta
    18541 actgtgtatg aagtgcttcg gcatctgctt ctgaatatat cgctacggtt tggatcccta
    18601 gttcgcggca tgcacgaatg atacgtacag cgatttcacc gcggttagct atcaatactt
    18661 tagaaaacat atttcaccta tccaatcata aaggttaatt ccgcttcggc tactttttta
    18721 ccgtctactt tagcgattcc tttgcctatt ccggctgaag ctttcacttt caatatctca
    18781 acttcaaggt ataaggtatc tcccggtgta actttcttac ggaattttgc tttgtccaat
    18841 ccgccgaaat aagccgtctt tcccttgaat tgttcaagag aaagtaatgc aactgctcct
    18901 gcttgagcca ttgcttcaac aatcaatacc cctggcataa caggttcttc tggaaaatgt
    18961 ccttgaaaaa agggctcatt gatcgttaca tttttcttag caacgatccg ttctccttca
    19021 accatttctt ccactcgatc aatcaaaagc attggataac gatgtggaat gatttctttt
    19081 atttcttgta tgttcattgt tttccctctt tcctgctttg cttatttttg ctgaatccta
    19141 aagagtggct gtccatattc gacaactgac tcgttttcta caaggatttc tgtgatcact
    19201 ccgtcggcct cactagttat ctcattcatc agtttcatcg cttcaataat gcagataact
    19261 tctcctttgg caacttgatc acctactttt ttgaaagctg gtttgtcggg cgctggttgc
    19321 agataaacga ttcccacgat tggcgaaaca acctcgattc catcaaatac tgccgattct
    19381 ttttctttca ttatttctgg agcagttgtt gcttgttcag gtatgacatt gactggctcc
    19441 gaaactggca acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt tttattttta
    19501 ttcatataca attcaaattg accttcacgc aaatcgaatt ccgttagcga ggattgatca
    19561 aattgactga ctaattctct gatttcattg atatccaaaa ttattcctcc cagcgtttca
    19621 tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt actcattgca taagtaaaac
    19681 ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat ctcaggatct tgtttttcta
    19741 cattgatcgt aggtgggata aattgatgtt ctaatgccag caaagttgcc aaggcttcga
    19801 tcccgccggc tgctcccaat aaatgtccag tcatcgattt cgtgctgctg acgtaagtat
    19861 ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg tcattggctg
    19921 gtgtacttgt tccgtgagca ttgatatatc cgacttgttc cggctgtaca ccagcttctt
    19981 ccatcgcaag cttcattgct ttagatgcac cagaaccatc tggagtagga gctgtcatat
    20041 ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc atagattttg gcaccgcgag
    20101 cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc tccttcaccc atcacaaaac
    20161 cattccgatt ttcgtcaaac ggaatcgaac cacgattagg atcagttgat tctgagagtg
    20221 ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat cgttgcttct gttcctccag
    20281 caagaatgac atcagaatag ccatgtttga tgttacggaa tgcttcccca attgaattcg
    20341 ttccgctagc acaagctgtt acgatagctg tacaaatacc ttttgcacct acacgcaaag
    20401 caatatttcc agctgccatg ttggcaatcg ccattggtac gaacattgga gaaactcgtt
    20461 ttgcaccttt ttcatgtaga cgaataactt ggttttcgat tgttggaaga ccaccgatcc
    20521 cagatccgat gatgacgcca aaccggtctt gattcatttt ttctttatct aaaccgctca
    20581 tctccattgc ttctaaagca gcgtagattc catagatcga aaacatatcc attcgtttgc
    20641 tatctttttt aataaaatat ttatcgtaag ggaaatcttt gacttcaccc gctaaggtaa
    20701 tccctgtttc tgaagcatca aatttagtga tcggtgcgat gccattcgtc ccctctttta
    20761 agctattcaa aaagctgtct gcgtcatttc caataggtga agtgactcca tagccagtaa
    20821 ttaccactcg attcatttgt ttgctctcct tttcttatcc gtgcatgacc aatccgccat
    20881 cgacattgat gacttgtccg gtgatatatg gattttgtgc taagaaaact gctgtttgtg
    20941 caatatcttc tacttgtccg aaacgctgca tcggaatttg cttttccgct tgttccttga
    21001 ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca atggcattac
    21061 atgtaatgcc tctagttgca gcttctcgtg ccacagattt tgttaatcct actacgccag
    21121 ctttactagc tgcataattt gcttgaccaa tatttccaat caacccagat acactagaga
    21181 gattaatgat ggccccttct cgctgcttca tcattttttt caatacgtgc tgcgtcatat
    21241 tgaaggttcc aatcaaattg atatccagac attttttgaa atcttctgca tccattcgca
    21301 tcacaagttt gtcatttgtg attccggcat tgtttactaa cacatggatc gatccaagct
    21361 tttcttctgc ttctttgatc atttgtcccg ctttctcata atcagagatg tcaccggaaa
    21421 ctcctacgca ttttacaccg aatgcttcga tttcttctat cttttctttt ggaatctctc
    21481 cgcgaccatt caaaatgata tttgctccag cttttgcaaa agctaaggcc atcgctttgc
    21541 cgattcctct tgtactacct gtgataaata cattttttcc ttttacatcc ataacgttcc
    21601 tccttatagt attgctattg tttcatcaaa tgtctgctta ttctcaacac gcagaactgg
    21661 tattgtttta tcgatttttt tcataaatcc gcttaatact tttccggggc cgacttcgat
    21721 gacttgttcg atgccgattg tttttaactt atggatactc tcatagaaac gaacaggttg
    21781 catgacttgc tgttcaagta gtcccgcgat tgtttctttc ttcattattt ccgttgttgt
    21841 gttgcttatg ataggaaaag aaggttcaga gaattgaatc tgacttaggt ccttcgccag
    21901 tttttttgct gccggttcta aaatagctgt atgaaaaggc ccgctgacat ttagagggat
    21961 cattcttttg aacccttttt cttttaatag taaaacagct tcgtctactg ctttttcttc
    22021 gccaccaatc acgatctgct gaggtgtatt ataatttgca ggagatacga tgccgtactt
    22081 gctagcttca tgacagcttt cctcgatcgt ttcaatcggc gcattcatca ctgcaaccat
    22141 tttcccgctt ccagctgggg cagcttctgt catataagcg cctctttttg ctactaacgc
    22201 aactgcttca gtgaaggata gcgcattact agctactaat gccgaatatt ctcctaaact
    22261 cagtcctaaa gctgcatctg ggatgattcc atgctccttc aataagcgat aatacgcgat
    22321 gctgactgtt agaatagctg gctgtgtata ttgcgtctga tccaaacgtt cattttctgt
    22381 aaagcaaagt tcagccatat cgtagccgag aatctcactt gcttcatcaa atgtctgctt
    22441 tacaacggct tcttcatata aatcttttcc cataccttga tattgtgcac cttggccact
    22501 aaataagaat gctgttttca tgtttttctc caatatatcc agaatatatg tttgattctt
    22561 cctattcaaa aaggccgaga cattcctatt tcaggaaatg tcccagcccc caacctaatt
    22621 ttaatgatta tgcagtttgt ttttctacgt aagtcactaa atcttgaacc gttttgattc
    22681 cgtcttctgt ttcgattttc acgtcaaatt catcttcaat ttcattgatg atttggaata
    22741 aatccaagct gtcagcttct aagtcttctt ggatgtcagt agtcaatttg atttcttcag
    22801 tttcttttcc taattcttct gcgatgattt cttgaatttt ttcgaatacc ataataatat
    22861 attcctccga tattgtttgc tttttatttt tataatttaa cagtctacaa ctccatgaga
    22921 atacttcccc atgttaaacc tccgccatag cctgtaaata ttactttctg ctgttttcct
    22981 aaggtgatct ttccactaga aactgcttca tccaataaga tagggatgct tgcagcagat
    23041 gtattcccat atttatccat attggtcagg aacttttctt gcggcatctt tacttttcgg
    23101 gcaattttct caataattct gaggtttgcc tgatgcaaaa gcagataatc aactggtttg
    23161 tctttcatga cttcttggat attgtttgca acgtctcgta cggcaaaatc aaaaatatct
    23221 ctgccagtca tttgcaaata atacgaacct tctgaatctt ctgaatagaa cggattttta
    23281 ttttgctggt agccagcggt caatgagcta cttctggtac cgtcagctgc taatttttct
    23341 ttcagcaatt ttttctgtgg attcgcttct aagagaatcc cacctgctcc atcgccaaac
    23401 aacacagcag tcgtacgatc agaccaatcg attagcttag atagtgtttc accaccaatc
    23461 accagtccct tagttgagcc gcagcggatc attttttcag ccatgcttaa cgcataaaca
    23521 aatcctgagc acgcagcact gatatcaaaa gcgatagcat gagttgctcc caatttccct
    23581 tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg acattgttgc tactaaaata
    23641 aaatccaaat ctttagggtt tagatttttc tgttttatca atttttccgc cactaaaatg
    23701 cataaatcag aagtctcctg atttgttaca catcttcgtt cacgaatacc cgtccttgac
    23761 gaaatccatt catcagaggt atccatcatc ttggaaagat cattattagt aataatcttt
    23821 tcaggaagga cgctggctgt tgcagtaatc attccaaatt gttccattaa atatcctcac
    23881 ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc atgctgttcc
    23941 tccgtagcca ttccgtctaa gatgttcttg accatttcac gatgaaaatg ctgatggacc
    24001 cgaaatagta actttccttt tttcgttagt cccaacttta caacgcggcg gtcatcttca
    24061 ctgcggatac gttcgacata gccttttttt actaacttgt taatagcagt ggtcaaggtc
    24121 ccaacagtaa ttgatagttc tttggctact tctgaagttg tttttttctt gtacatcccg
    24181 attgcctcga tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga ttttttcagc
    24241 tcagactctt cgattgtcaa aatatcattg aagacactaa caagataatc atttactgtt
    24301 tttaaatctg gttccatgtt ccaccgtcca ctaattagtt tgattatcaa atcttttgat
    24361 gttcaaacta tatcttaact tttttcaaaa tgcaacctta aaagatcatt tttttactat
    24421 atgagctttt tttaagattt tactcattta aaacaactga taaatcagca tttataattt
    24481 cttttattag aaaaatcatc tatctcacat attttgatta tcaaagtatt ttctgtgaac
    24541 agaactatag aattccctaa aacttgtcca ttatttcaaa atttgttata gtctttttat
    24601 tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc
    24661 gggtgtctat cttatgaaag accgagatgg taaggtcatc tatgttggta aagcaaaacg
    24721 gctcaaaaat agagtaagca gctattttca tcagaataaa cagcattcaa aaaaagtatt
    24781 acgtatgatt catcatataa cagattttga tttcgtcgta gtagacacgg aattagatgc
    24841 tttattgctc gagtgtcaat tgatccaaca ttaccgtcct ttttataacc gacaaatgaa
    24901 ttatttcagt aattataatt atgtacatat taccaataag ggatttgtat taacagatac
    24961 tcctacggct cgaacatacg ggccttttcg tctctataaa aaaatgccgt ctattttgcg
    25021 tataatggaa gaaacttatc aaatgccttg gttgtctgaa attagtttgt tagctttgcg
    25081 agtccagctt cctgacctac aggaaatgac ttttgaacag aaaaaaaagg aactccaagg
    25141 gctgtttcaa ggccgaaata aaaagctact gacttacttg aaaaaaagac aacaacattt
    25201 catctatcag ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg aattagttac
    25261 ttattttatt agacggatcc aggaacaaaa gcagttttta agaacaccat cgctcacctt
    25321 cagcatgcca ttagcagcag atgagagtca gaaaaagcac tacctcgttt gttatggtca
    25381 acttgcagaa acaatgatta cctctggtga cgtttctcct gacttctatt atgaaaagaa
    25441 agaagctcat ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc ctgtacaaat
    25501 cttgatcagt taccgcaaaa aattagaaaa agaacagttt gaaattgaac tgttaaacaa
    25561 aaaagaagct gaaaagcaac taaattagtt gcttctcggc ttcttttagg catcttcaac
    25621 ttcaaatgtc aattatccta aaatttattg gattaagtca tagatttcca tcgctacgat
    25681 atcgatatta tcaaattcgt attgctgatg agtatctgtt tcagttaatt caaatgtatc
    25741 ggtcgcttta tcataagtta ctgtacactt ctctacgcct tctttttcaa aacggcgaac
    25801 ttgcagttca ttatctgctg actcgaccat agcctccagt cgttttatga ttgcgactaa
    25861 ttgtgaatgt gtcatcggag gtcctccttt tcttatttct gttatattgt atcaaaagca
    25921 tagcctactt gcattgattt tctctaaaaa aactcgtttt ttatgatttt ttttctggta
    25981 tttttccttc aggccaccaa agtttgatca aagcttgggt tccgtcattc tcaaaacctt
    26041 tatctgccaa ctcttcatat agtttcaatg cttgggtagt tgctggcaat acaagatcca
    26101 ttttttctgc ttcttctaat gcaatcttta aatctttgat aaaatgtttg acaaaaaaac
    26161 caggtgtgta atcttctttt aaaatacgag gagaataatt gcttaaagac cagttagctg
    26221 cacttccacc tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt ttttgtgcat
    26281 agacaagcat ttccgtcatt cctgtcatcg tacctgcaat catgatctga ttagccattt
    26341 ttgtatgctg acctttccca gcagaaccat gcaatgtaaa tgtcgtacca aattctttga
    26401 ataaaggcag tacttgatca taaacggctt cttctccacc gaccatgatc gtcagtgtac
    26461 cattctttgc tcctaagtca ccaccggata ccggtgcatc taacgcatct gcgccttttt
    26521 cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc aaatcaacta
    26581 aaatatgtcc tgagatatct gcagaaaaaa tccccgactg tccatagtag atttcttcta
    26641 catctttggg gtaacctacc attgtaaaga gaatattact ttgttctgct accgcttttg
    26701 gtgtatctgc ccagatagct ccttctgtta ctaaaggatc agcttttgct tttgtccgat
    26761 tatagacagc tacttcgtgt cctgcttcta gtagatggcg tgcgacagca cttcccatga
    26821 cgcctgttcc gataaaacca agtttcattt tttcgacctc ctaaaaatca aaaatccttg
    26881 cttctatgct agtatacgct agaaacaagg attttttcat aaaaaatgtt tgaattttat
    26941 cttttaaatg aatttaattt tattttgatt cgttcaatcg agtcttcttc ttcttttctt
    27001 cgcatctctt cttcatgata aacaaccatg tcttgataaa attcgatcat agcttcgccg
    27061 aaatgtgtag atgaaatatc ataaagttta gcttctaaaa cttttggatc tttaggaact
    27121 tcactggaaa tataatcaat aagtgctggt gcaaagtctt cgtcggtttc aaaagttttc
    27181 cctaatgaag gatgatcgaa caatcggttc aaataagcat ttccttccac gacacaaggt
    27241 gttccggctg ccatcgcttc tgtataagta agcccttgtg tttctgaagt agaagcactg
    27301 acaaaataat ctgccgcttt ataataaagt gctacatcat tgttgtccac ttctcctgtg
    27361 aactgcacaa attcttctaa ctgcagttct gagatcaatt cctctaactc ttctacataa
    27421 ggtccttttc ctacgataac taatctggcg ttaggataag cggagatgac ctgaggaaac
    27481 ccattaatca aggcttggat atttttctca taagaaatcc tgcttaaaga aagaatcatc
    27541 aaatggtttt cctgaagacc tagtgactcc cttaatttgc tgatatcctg ctgagtgata
    27601 tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag cggtgctatt
    27661 acaccatagc tgcgtaatgt atcaataaca cgttcacttg gacaaactac acctgtcgta
    27721 tgatttgcaa ataatctaga caagtatttt acatgggatt gcctgatcac ttttccttta
    27781 ccgatataat gaaggtagtc ttcatacatc gtatggtaag tatggatcag aggtattttc
    27841 aacttcttag ctaccatctt cccaagtatc cctgctccga attctgtatg ggtatgaata
    27901 agatctagtt ctagttcttt ggcaaccaaa taagcatacc acatgcctct aacaacaact
    27961 cttcgatcct tgaaagaaat aaatggtaca cttggcattc gaatcacatc tttttccaac
    28021 ttatccgcat taggatcggt cgttgtaaaa atatagactt catgcccatg tctttctaat
    28081 tcttgtttta atgttttgat cgatgtagca actccgctga cttgcggaaa gtaggtatcg
    28141 gtaaaaaagc cgattttcat gtttttcgcc tccaaactag tccttgacct gatttacttt
    28201 ttttaatttg ctagagactt tttcattcag tacactttcg tatacatttt taagttcttc
    28261 accgatttta ttgatacttc gctcttttgc tgtttgataa ccggcttgtg tcgtactcgg
    28321 tagttcttga ttggtaatct gttggatcag tcggataaat tcttcgtttg tcttccccat
    28381 atagcagttc ttgctatctt ctaaccagcc attatacact ggaatatctc gaacaagtac
    28441 gttttgtttg cttgctaaag cttctaaaac aacgatccct tctgtctctt cataagaagg
    28501 gaaaaagaat agatctgcac ctgaatacgc accttctatg atctctccac gaatgtaacc
    28561 aggaaacagg acattttcag gatgatcttc ttttacaatt ttccggatgt tccgaggaat
    28621 cgagtacatg ggaacatgac caaaccaaat aaatgtgtat tcgggcatct tcttagcaat
    28681 ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa tgattacttt
    28741 ttgatccgga gacagtttga aatattctct gaacttttgt tctttcattg gatcagggtg
    28801 atatttctct aagtcgatcc cattggaaat agcttgaatc ggcacacgga taccatagct
    28861 ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt tttggtacaa
    28921 tcctaccaga tattttttta cgataggaga aagctggttc gatccgataa aggaatttcg
    28981 gaaatcttcc tctgtagaat gcgcatgata gacaactttt tttccttctc ttctggcttt
    29041 attgaccata ttatgactat tgattccgta tgtattgatg tgcaggatgt cataatcttc
    29101 tttttcatct agtgtataag agataccgac ttccgtcaga gcacgttttt gatgatcaag
    29161 cgcacgcccg attccagatt ttgcaagcat cttttcactt tcaaaatata ataatacctt
    29221 caccttacgt cccccttcac ttatatagta taacatagcc tacaaactgt cactcaaact
    29281 taagccctat tt
//
LOCUS       DACDCV010000026        28194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDCV010000026 DACDCV010000000
VERSION     DACDCV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.026"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(205..1104)
                     /locus_tag="IV041_001141"
     CDS             complement(205..1104)
                     /locus_tag="IV041_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8381950.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="IV041_001142"
     CDS             complement(1114..1470)
                     /locus_tag="IV041_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8381951.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="IV041_001143"
     CDS             1825..2121
                     /locus_tag="IV041_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8381952.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="IV041_001144"
     CDS             complement(2765..3718)
                     /locus_tag="IV041_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8381953.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="IV041_001145"
     CDS             complement(3741..4496)
                     /locus_tag="IV041_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8381954.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="IV041_001146"
     CDS             complement(4493..5452)
                     /locus_tag="IV041_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8381955.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="IV041_001147"
     CDS             complement(5445..6395)
                     /locus_tag="IV041_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8381956.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="IV041_001148"
     CDS             6545..6997
                     /locus_tag="IV041_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8381957.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV041_001149"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV041_001149"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8381958.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV041_001150"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV041_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8381959.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="IV041_001151"
     CDS             complement(10549..11358)
                     /locus_tag="IV041_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8381960.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV041_001152"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV041_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8381961.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="IV041_001153"
     CDS             complement(14968..15654)
                     /locus_tag="IV041_001153"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8381962.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="IV041_001154"
     CDS             complement(16002..17783)
                     /locus_tag="IV041_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8381963.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="IV041_001155"
     CDS             complement(17809..18723)
                     /locus_tag="IV041_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381964.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="IV041_001156"
     CDS             complement(18750..19712)
                     /locus_tag="IV041_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381965.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="IV041_001157"
     CDS             complement(19712..20656)
                     /locus_tag="IV041_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381966.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="IV041_001158"
     CDS             complement(20657..21661)
                     /locus_tag="IV041_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381967.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IV041_001159"
     CDS             complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IV041_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8381968.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IV041_001160"
     CDS             complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IV041_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8381969.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IV041_001161"
     CDS             complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IV041_001161"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8381970.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25496..27178)
                     /locus_tag="IV041_001162"
     CDS             complement(25496..27178)
                     /locus_tag="IV041_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8381971.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27230..27592)
                     /locus_tag="IV041_001163"
     CDS             complement(27230..27592)
                     /locus_tag="IV041_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8381972.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27862..28050
                     /locus_tag="IV041_001164"
     CDS             27862..28050
                     /locus_tag="IV041_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8381973.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7799 a         6089 c         4960 g         9346 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct gcatgcagct
    21901 ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata taatctactg
    21961 cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta ccgaattcat
    22021 cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa tcatctttga
    22081 tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa ataattttcg
    22141 ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat catacacttg
    22201 tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc accttttttt
    22261 caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc aaattgttcg
    22321 actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat cgtatagcga
    22381 acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa
    22441 acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa gccgtctttt
    22501 aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga ttttaataga
    22561 ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca
    22621 tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac gtttccaata
    22681 aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac ttcactacct
    22741 tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat acctcggact
    22801 ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg tttgttttct
    22861 gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac tactggcaag
    22921 gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa caaccctgct
    22981 gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc gtttttcact
    23041 gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt tactggctca
    23101 tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc atttgttaag
    23161 tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc
    23221 tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc cattgcatct
    23281 acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta tagcacagtc
    23341 tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc taaaggctga
    23401 tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc ttctctggct
    23461 acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg cagaccagat
    23521 tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc gcttagtaca
    23581 aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc atttttagga
    23641 ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc acgaagttgg
    23701 tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt cgcgttcggc
    23761 acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg gttgtctttg
    23821 aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa caagccaact
    23881 tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc attttttaca
    23941 tctaatgctt ctgattcttc aatcagcgga cagatgacat acatttgatg tcctcttgcc
    24001 agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac ccagcgtgtt
    24061 tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat ctcaccatat
    24121 gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac atctggtcgc
    24181 cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg ctcatccgta
    24241 ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg tgtgccgatc
    24301 acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc ttttgctttc
    24361 gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg ttgcaagctt
    24421 tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg gaaacctgct
    24481 gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc tacatctccc
    24541 tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc attcgttaca
    24601 cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt caatttttgg
    24661 acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt caatccttga
    24721 agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc ttgatggttt
    24781 tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc aagcagtcga
    24841 tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta ttcttgaaac
    24901 ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac gtgatatatc
    24961 ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa ggctttacgc
    25021 tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt ttcttttaga
    25081 taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat acgaaattgc
    25141 aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac gattcctttc
    25201 aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat atcttcgtag
    25261 cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct ggcaagacta
    25321 gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa agaacgcata
    25381 aatttttcct ccctccttta acaaaaaaag gacttggtca gcgatcatac ctgatcacta
    25441 accgaaggcc ttttatgaat gaatactgtg ttagaaagat attctgacac agaaattatt
    25501 cagcagaaaa tagataagga tagacaggct gatctccttg gtggatctcg atttccaaat
    25561 caggatgttt ttctgttagt ttctcagcaa attcatttgc ttgttcaact gttccttcat
    25621 cacctgccaa gatcgttacg atctctgtgt cctcattgat catttgttct agtgtactta
    25681 acgctgttgc tgtgatatct gggttagaga tcaggatttt tccatcgatc atgcccaaaa
    25741 agtctccttc tttgatctct acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt
    25801 gaccgctcac aacgctagac aacatttctg tcatcgtttc tttgttttct tctaaagaag
    25861 cttgatcatt gaatgaaagc atagctgtca atccttggga aatcgttctt gaaggaacaa
    25921 ctgctactgg gatatctgct acttctgctg cttgatcagc tgccatgaag atgttcttat
    25981 tgtttggcaa gacaatcact ttatctgcgt ttacttcttg aatcgcttta acaatgtctt
    26041 ccgtacttgg attcattgtt tgtccaccac tgataacata attagcgcct aaactttcaa
    26101 acagctcttg gacgccttta ccagcagcga tcgctacgat accatacggg atacgtggta
    26161 tttctttttt cgcaaactcg ttcacttctt catcgtgttc caaaatcgtc tcatgctgca
    26221 agcgcatgtt gtcgactttg attttgacta atgaaccgaa tttttgtcca taattcaaga
    26281 cttctcctgg gttttctgta tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa
    26341 gtaaagaatc tcctatacca tctaaatagt tacggaattc ttcgtaatcg aatgtactat
    26401 cgactgtagg tccttctccg atttttacca tgatttctgt acagtaacca aaatggatat
    26461 cttctgtcgc catttgacct tggacactgc gatgatgttc tgcattgacc atatcatcca
    26521 tttctgctgg agaaggttca tatgtttcat ccgcttggaa ttctccattc aatgcactta
    26581 aaaagccctc ataaacaaac agcagtcctt gccctccact gtctacgaca ccgacttctt
    26641 ttaggactgg aagcaagtct ggagttttag ctaaagcacg ctttgctcct tttacaacag
    26701 ctgtcattac ttcaacgaca tcatcagtac tttttgcttt cttttctcca gctttcgctg
    26761 cttctctagc tactgtcaaa atcgttcctt ctactggttt cattactgct ttatacgcag
    26821 tttcaactcc atgagtgaag gctgcagcaa gatcagaagc atttaatgag acaacatctg
    26881 gaatttgctt agaaaatcca cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg
    26941 cacccatcag caatcctttt gaaagcattg ctgctaattc tcctactttt tctgaggttg
    27001 aatctactac tgccttagct ccactagtca tagatagatt catatttgtt cccgtatcgc
    27061 catctggcac agggaataca tttaatgaat tgacgtattc tgcatttact tgcagacgat
    27121 tcgcacctgc ctggaccatt tcctggaact ggcttgcact tatttctgtt acactcacct
    27181 agataatcct ccttcagatc gactgctgag ttaccagcca gtctcctgcc tagtctggca
    27241 gtacacgaac accttgtaca aagacattaa ctgaattggc agttacgcct aataatgcct
    27301 ctagattgta tttcactttt tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat
    27361 agctgataat cgtataaaca tctacagcaa taccattatc ttcttggcgg acaactactc
    27421 ctcttgagta attttcttta cgcaaaatct cagtaatatt atctttaatt tgatttttgc
    27481 tggccattcc tacaacaccg aacacatctg tcgcggcgcc accaacaact gttgcaatta
    27541 catcattggt aatttcaatg gttccagctg acgtttgaat ttttacagcc atgaatgagc
    27601 ctccttaacg ggtaaaaaac tcccattttt cttctatcat attttatcat agcgcaactt
    27661 gaaataaaag gcagttaccc aaagtactca aaaaccttgc aaaatttcag gtataacctt
    27721 gtttaattta caattattct tctcaattgt caattgtatc tatgaaaatg tacttgcaaa
    27781 ttcccattct atatgatatg ttagtctggt atgagctgaa ttattctatt gctccaaatc
    27841 aaggcaaagg aggaaattac catggcaaaa gtttgttact ttacaggtcg caagacaaaa
    27901 agtggaaaca accgttcaca cgctatgaac tcaacaaaac gtactgttaa acctaactta
    27961 caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct
    28021 ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata cttggaccgt tcagatagtg
    28081 aacggttttt tatttaataa aaatttttaa gggtgggact tttgattcct accctttttt
    28141 tgcgtttatt tttgttatac gaacaaatta tgaagttatt tattctaaat ttta
//
LOCUS       DACDCV010000027        26810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDCV010000027 DACDCV010000000
VERSION     DACDCV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.027"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..212)
                     /locus_tag="IV041_001165"
     CDS             complement(12..212)
                     /locus_tag="IV041_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8381974.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="IV041_001166"
     CDS             complement(517..1749)
                     /locus_tag="IV041_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8381975.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2003..2572
                     /locus_tag="IV041_001167"
     CDS             2003..2572
                     /locus_tag="IV041_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8381976.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2750..3190)
                     /locus_tag="IV041_001168"
     CDS             complement(2750..3190)
                     /locus_tag="IV041_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8381977.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3348..4112
                     /gene="map"
                     /locus_tag="IV041_001169"
     CDS             3348..4112
                     /gene="map"
                     /locus_tag="IV041_001169"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8381978.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4144..5067
                     /locus_tag="IV041_001170"
     CDS             4144..5067
                     /locus_tag="IV041_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8381979.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5143..6282
                     /locus_tag="IV041_001171"
     CDS             5143..6282
                     /locus_tag="IV041_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8381980.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6275..7075
                     /locus_tag="IV041_001172"
     CDS             6275..7075
                     /locus_tag="IV041_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8381981.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7075..7902
                     /locus_tag="IV041_001173"
     CDS             7075..7902
                     /locus_tag="IV041_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8381982.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7880..8614
                     /locus_tag="IV041_001174"
     CDS             7880..8614
                     /locus_tag="IV041_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8381983.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8714..9580
                     /gene="rfbA"
                     /locus_tag="IV041_001175"
     CDS             8714..9580
                     /gene="rfbA"
                     /locus_tag="IV041_001175"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8381984.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9594..10166
                     /gene="rfbC"
                     /locus_tag="IV041_001176"
     CDS             9594..10166
                     /gene="rfbC"
                     /locus_tag="IV041_001176"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8381985.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10188..11216
                     /gene="rfbB"
                     /locus_tag="IV041_001177"
     CDS             10188..11216
                     /gene="rfbB"
                     /locus_tag="IV041_001177"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8381986.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11314..12165
                     /gene="rfbD"
                     /locus_tag="IV041_001178"
     CDS             11314..12165
                     /gene="rfbD"
                     /locus_tag="IV041_001178"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8381987.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12200..14233
                     /locus_tag="IV041_001179"
     CDS             12200..14233
                     /locus_tag="IV041_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8381988.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14277..15557
                     /locus_tag="IV041_001180"
     CDS             14277..15557
                     /locus_tag="IV041_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8381989.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15767..16573
                     /locus_tag="IV041_001181"
     CDS             15767..16573
                     /locus_tag="IV041_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8381990.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16585..17805
                     /locus_tag="IV041_001182"
     CDS             16585..17805
                     /locus_tag="IV041_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8381991.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17795..19381
                     /locus_tag="IV041_001183"
     CDS             17795..19381
                     /locus_tag="IV041_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8381992.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19420..21558
                     /locus_tag="IV041_001184"
     CDS             19420..21558
                     /locus_tag="IV041_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8381993.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21926..22909)
                     /locus_tag="IV041_001185"
     CDS             complement(21926..22909)
                     /locus_tag="IV041_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8381994.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23287..24678
                     /locus_tag="IV041_001186"
     CDS             23287..24678
                     /locus_tag="IV041_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8381995.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24694..25734
                     /locus_tag="IV041_001187"
     CDS             24694..25734
                     /locus_tag="IV041_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8381996.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25753..>26810
                     /locus_tag="IV041_001188"
     CDS             25753..>26810
                     /locus_tag="IV041_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8381997.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQ"
BASE COUNT         8568 a         4486 c         5452 g         8304 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     1861 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     1921 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     1981 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2041 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2101 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2161 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     2221 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2281 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     2341 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2401 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2461 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2521 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2581 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2641 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     2701 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     2761 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     2821 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     2881 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     2941 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3001 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3061 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3121 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3181 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3241 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3301 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3361 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3421 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3481 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3541 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3601 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3661 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     3721 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     3781 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     3841 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     3901 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     3961 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4021 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4081 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4141 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4201 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4261 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4321 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4381 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4441 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4501 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4561 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4621 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4681 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     4741 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     4801 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     4861 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     4921 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     4981 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5041 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     5101 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     5161 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     5221 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     5281 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     5341 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     5401 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     5461 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     5521 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     5581 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     5641 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     5701 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     5761 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     5821 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     5881 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     5941 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     6001 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     6061 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcgt attgattatt
     6121 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     6181 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     6241 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     6301 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     6361 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     6421 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     6481 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     6541 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     6601 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     6661 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     6721 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     6781 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     6841 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     6901 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     6961 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     7021 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     7081 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     7141 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     7201 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     7261 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     7321 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     7381 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     7441 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     7501 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     7561 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     7621 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     7681 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     7741 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     7801 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     7861 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     7921 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
     7981 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
     8041 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
     8101 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
     8161 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
     8221 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
     8281 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
     8341 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
     8401 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
     8461 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
     8521 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
     8581 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
     8641 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
     8701 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
     8761 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
     8821 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
     8881 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
     8941 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
     9001 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
     9061 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
     9121 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
     9181 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
     9241 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
     9301 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
     9361 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
     9421 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
     9481 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
     9541 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
     9601 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
     9661 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
     9721 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
     9781 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
     9841 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
     9901 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
     9961 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    10021 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    10081 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    10141 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    10201 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    10261 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    10321 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    10381 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    10441 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    10501 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    10561 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    10621 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    10681 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    10741 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    10801 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    10861 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    10921 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    10981 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    11041 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    11101 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    11161 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    11221 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    11281 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    11341 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    11401 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    11461 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    11521 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    11581 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    11641 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    11701 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    11761 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    11821 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    11881 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    11941 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    12001 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    12061 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    12121 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    12181 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    12241 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    12301 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    12361 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    12421 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    12481 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    12541 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    12601 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    12661 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    12721 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    12781 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    12841 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    12901 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    12961 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    13021 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    13081 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    13141 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    13201 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    13261 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    13321 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    13381 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    13441 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    13501 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    13561 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    13621 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    13681 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    13741 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    13801 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    13861 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    13921 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    13981 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    14041 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    14101 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    14161 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    14221 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    14281 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    14341 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    14401 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    14461 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    14521 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    14581 ctttggttat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    14641 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    14701 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    14761 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    14821 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    14881 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    14941 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    15001 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    15061 tcaatttgtt taccgatcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    15121 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    15181 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    15241 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    15301 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    15361 atacattgac ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc
    15421 atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg
    15481 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    15541 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    15601 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    15661 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    15721 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    15781 gtatttattt atgaaggaca tttatcagaa cagaaaatta ttgctacagt tttcgatcaa
    15841 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    15901 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    15961 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    16021 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    16081 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    16141 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    16201 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    16261 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    16321 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    16381 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    16441 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    16501 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    16561 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    16621 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    16681 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    16741 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    16801 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    16861 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    16921 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    16981 gaatttgctg acatcggaga ccatatttat caacctgtaa aaacttattc aagcgggatg
    17041 ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc ccgatatttt gattgtggat
    17101 gaagcattgg cagtaggcga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    17161 atcaagaact cgggaaagac gattttattc gtttctcatg atatcagttc gattcgtcgt
    17221 ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg
    17281 gaagtaacga cgaattatga aaacttttta aagaaaaaat cagttaaaac agtggatcgg
    17341 aataaaaacc aagtagaacg gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa
    17401 ttgttggata aagacaaaaa tccggtggat atgctggaac aaggagaaaa actatacgtt
    17461 aaagtaactt atgaagtaaa agatgataca atcaaaaatc cggttttagg aattgcgcta
    17521 cgaacagtcg acaattttta tgtatgcgga cttaatactc tactagatgg tatcaagatt
    17581 ccttggaaaa aaggagagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    17641 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatat
    17701 aagacaaagt acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    17761 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    17821 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    17881 agagtttggt cccggaaacg gccgattaac gaaacattta atcggagcaa aacaatgtga
    17941 agtgagcatt gttgagttag ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga
    18001 tggattttat ggtgatatcg aaagttttga gtgggcaaac tattatgctg gacagacatt
    18061 tgattacgtc ttgtttgcgg atgttttaga acatttggta gatcctggaa aaacattgaa
    18121 gaaagtcaga gaatttttga atgaagaagg agagatcctg attacattcc ctaacttggc
    18181 gcataattct gtcatgatcg atttgttcaa taaccaattg ccgtgggctt cttatggtct
    18241 cctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    18301 agcgggatta ttcattaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    18361 aaaatcaact tatgaagaat tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt
    18421 tggtgaagtc taccaatact ttttctcatt gatgaaacat ccagtagcac aaagttccat
    18481 tgcagagcca caaaattcta attatgtaaa agtgttagaa gtaacccagc aaacgaaaca
    18541 agatgaaaca atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    18601 tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt
    18661 tatcgaattc agcgcagaag ctgccggaga aaaactaaca tttatcgatt cgaatgctgt
    18721 agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa gaattaccag agttcgttct
    18781 aacggatatt tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt
    18841 gacaccaaca atgaaagaat tgttggaaac gattcgtccc atggctgaag tcacaaagca
    18901 gctttctgag aaaaatgacg aattagaaag agaaaatcat catcttcttg aggaaaatac
    18961 aagactagac catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga
    19021 tgaatggaca ataaaacata gactaggacg acgtaagaaa gaaacttcga aaaaaatcca
    19081 agaaaaagaa ctttctatat gtattgacga gaaaatctgg gatgcagaaa caaaaatatt
    19141 aaaaatcatc ggatggggaa tcgctaattc ggatcgccag cccttgtcct ataagttatc
    19201 agctgaccaa tcgccatttt tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc
    19261 agaacagctc gcaaaaggaa cagaagcggg atttgagtta cgaatccttt gcgagcaaga
    19321 acgttcattc ttagtggaag cagtagctca aaatggccaa tcatggatca ttgaaatgta
    19381 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    19441 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    19501 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    19561 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    19621 acccaaagcg gtttcaaggt acgtttatca gggaaaatga gaggaaaagc catcttagac
    19681 ttccaaacag cgagacatca aaatggcata gctgtgaaat taaacggtaa atacccttat
    19741 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    19801 tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg tcaaattgga attgaagcca
    19861 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aaccagattt gaaagagcaa
    19921 aggaaagaag cacaaggatt tgcgtaccgt ccattgattt ccatacttat gcctgtctat
    19981 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tactggatca aacgtatgat
    20041 cattgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg
    20101 gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt tccgaaaaga aaatggacac
    20161 atcagtctgg caactaattc tgcattggaa atagcagaag gagaatttat agcactctta
    20221 gataatgatg atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacgta
    20281 catccagaac tggatgtgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc
    20341 tttgatccgc atttcaaagc agactggtcg cctgatacat tgatgggaaa taattatatc
    20401 tctcacttag gcgtatatcg aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc
    20461 tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat
    20521 catatttacc acattgaccg agtcttgtat cattggcgga cgatcccagg atcaactgca
    20581 agtaatggcg aagccaaaag ttatatctat gattccggag tgaaagcatt gacagatgcc
    20641 ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag
    20701 attgcttatg acgtcttgca agaggatttg gtcagtgtga tcattccaac gaagaatggc
    20761 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta ttcaaattat
    20821 gaaatcatta ttgcggataa cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa
    20881 tacaagcatc agctgaaaga ccgctttatc gtagaattga ttgacattcc attcaattat
    20941 tctcgaatta acaatttagc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    21001 aacgatacag aagtgattga gccagattgg atgacggtga tggtttctta tgcacagttt
    21061 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
    21121 ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca
    21181 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    21241 tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa
    21301 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    21361 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact
    21421 ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca
    21481 tatattgcgc atgatccatt ttacaatgat aatttaacaa aagacggaat cggcgacttt
    21541 tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata
    21601 acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt
    21661 cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt
    21721 ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta
    21781 ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg
    21841 ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag
    21901 aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta
    21961 caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat
    22021 tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt
    22081 tctcagaatc taatacccac cataaatagc catatctcat attttgtaag tcttctaaag
    22141 cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt
    22201 tatacatccc tttatttagg cataatacac caatttttgc cagatcactt gccgataaac
    22261 acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag
    22321 gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat
    22381 ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct
    22441 tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt
    22501 cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac
    22561 ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca
    22621 agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa
    22681 aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa
    22741 tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat
    22801 aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata
    22861 tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc
    22921 tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca
    22981 cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa
    23041 tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt
    23101 taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga
    23161 ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa
    23221 actaggtttt taaaggtgac tatggtaaga taatagagat attattttgt gattaggagt
    23281 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
    23341 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
    23401 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
    23461 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
    23521 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
    23581 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
    23641 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
    23701 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
    23761 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
    23821 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
    23881 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
    23941 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
    24001 attgaagcat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
    24061 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
    24121 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
    24181 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
    24241 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
    24301 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
    24361 gtaggaccac gtcctgaacg ttctttcttc gtcgatcagt tcaatgcaga aaacccttat
    24421 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
    24481 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
    24541 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
    24601 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
    24661 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
    24721 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
    24781 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
    24841 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
    24901 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
    24961 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
    25021 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
    25081 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
    25141 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
    25201 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
    25261 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
    25321 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
    25381 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
    25441 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
    25501 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
    25561 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
    25621 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
    25681 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
    25741 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
    25801 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
    25861 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
    25921 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
    25981 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
    26041 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
    26101 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
    26161 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
    26221 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
    26281 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
    26341 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
    26401 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
    26461 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
    26521 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
    26581 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
    26641 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
    26701 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
    26761 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat
//
LOCUS       DACDCV010000028        26248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDCV010000028 DACDCV010000000
VERSION     DACDCV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.028"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            615..1943
                     /locus_tag="IV041_001189"
     CDS             615..1943
                     /locus_tag="IV041_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8381998.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2145..2978
                     /locus_tag="IV041_001190"
     CDS             2145..2978
                     /locus_tag="IV041_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8381999.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2994..3731
                     /locus_tag="IV041_001191"
     CDS             2994..3731
                     /locus_tag="IV041_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8382000.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3886..4812)
                     /locus_tag="IV041_001192"
     CDS             complement(3886..4812)
                     /locus_tag="IV041_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8382001.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5087..5920
                     /locus_tag="IV041_001193"
     CDS             5087..5920
                     /locus_tag="IV041_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8382002.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5954..6793
                     /locus_tag="IV041_001194"
     CDS             5954..6793
                     /locus_tag="IV041_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8382003.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6819..7379
                     /locus_tag="IV041_001195"
     CDS             6819..7379
                     /locus_tag="IV041_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8382004.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7578..9299
                     /locus_tag="IV041_001196"
     CDS             7578..9299
                     /locus_tag="IV041_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8382005.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9464..10606
                     /locus_tag="IV041_001197"
     CDS             9464..10606
                     /locus_tag="IV041_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8382006.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10622..11833
                     /gene="thiI"
                     /locus_tag="IV041_001198"
     CDS             10622..11833
                     /gene="thiI"
                     /locus_tag="IV041_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8382007.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12406..13053
                     /locus_tag="IV041_001199"
     CDS             12406..13053
                     /locus_tag="IV041_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8382008.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13446..16091
                     /locus_tag="IV041_001200"
     CDS             13446..16091
                     /locus_tag="IV041_001200"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8382009.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16401..17714
                     /locus_tag="IV041_001201"
     CDS             16401..17714
                     /locus_tag="IV041_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8382010.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17704..18357
                     /locus_tag="IV041_001202"
     CDS             17704..18357
                     /locus_tag="IV041_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8382011.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18412..18963
                     /gene="radC"
                     /locus_tag="IV041_001203"
                     /pseudo
     CDS             18412..18963
                     /gene="radC"
                     /locus_tag="IV041_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19094..20179
                     /locus_tag="IV041_001204"
     CDS             19094..20179
                     /locus_tag="IV041_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8382012.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20176..22068
                     /locus_tag="IV041_001205"
     CDS             20176..22068
                     /locus_tag="IV041_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8382013.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22075..22452
                     /locus_tag="IV041_001206"
     CDS             22075..22452
                     /locus_tag="IV041_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8382014.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22606..23388
                     /locus_tag="IV041_001207"
     CDS             22606..23388
                     /locus_tag="IV041_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8382015.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23514..24245)
                     /gene="erm(A)"
                     /locus_tag="IV041_001208"
     CDS             complement(23514..24245)
                     /gene="erm(A)"
                     /locus_tag="IV041_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8382016.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24303..24390)
                     /locus_tag="IV041_001209"
                     /pseudo
     CDS             complement(24303..24390)
                     /locus_tag="IV041_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            24754..25416
                     /locus_tag="IV041_001210"
     CDS             24754..25416
                     /locus_tag="IV041_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8382017.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25651..25794
                     /locus_tag="IV041_001211"
                     /pseudo
     CDS             25651..25794
                     /locus_tag="IV041_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            25944..26144
                     /locus_tag="IV041_001212"
     CDS             25944..26144
                     /locus_tag="IV041_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8382018.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         8993 a         4204 c         5420 g         7631 t
ORIGIN      
        1 cgagtcctac tgccggagta aataaagctt atgcaaagaa aaagaaatcc aatcacttgg
       61 gtttcttttt cttttattat tatgatgaat caagctttct gtttttgtca cgtatgttgt
      121 aatctttacg aaagagtaga gttggctact cataatcaac catacttccc atcatcgcta
      181 aatacgaaat aaatcagcat tcctacacca atcaaagcac aagctgcacc aagaaaaggg
      241 atgacattcc caaagttatc tgtattcata agtatgccac cacccacaaa caaaaagccg
      301 ccagcaatca ggtttttcat tttattcatc tcctttctta cgttatagta tttccttctt
      361 tcaatgagta ttttgaaagc tatcttattg tatgatgttc ttctaggcct cttctttatt
      421 atacaggcca ttttcgttat ctcatagaaa tattcctttt tatctgaaaa ctattgtata
      481 ccgtatgaca tttttcttca tgaagttgta agtatattct cattcaaatg aaaatttcat
      541 catagaagag attgtatttc taaattctta agagtaaaat acgaagtata acttggattt
      601 ggaaggatga ttggatgaaa gctattacgc ctgatatgac aacaaaattt aacaaacagt
      661 ttcaagcaaa tatcaaacag caggcattgc agcgggcagt tgtgaagaac ggaattactg
      721 cttctgctga aaatgtttca gcacatgtgg agaatacacc ggtattttct atagatttgg
      781 ctacggggaa agtggctaat caaatgcaat cggggcgttg ctggatgttt gctgcactga
      841 acacattccg tcataagatg ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa
      901 attacacttt tttctgggat aaatatgaaa aggcaaatta tttctatgaa aacatcattg
      961 caacaggaaa agaggcaatc gatagccgga aagtagcttt cttactcgct actcctcaac
     1021 aagatggcgg ccaatgggat atgatcgttt cattattcca aaaatatggc gttgtgccta
     1081 aatcggtgat gccggaaagt gcgaatagtt caaattctcg agatttgaat aactacttga
     1141 acaaattact gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat
     1201 tagacgctat tgaagaaaag aaagaagaaa tgctagaaag catttataac ttcttagcta
     1261 tctcattagg aacacctcca aaagaattcg attttgagta ccgcgatgaa gaaaagaact
     1321 atcatcttga ccgaggactg acaccacaaa ttttttatga taaatacatc ggtgtaaaat
     1381 tagatgatta tgttagcgtg atcaatgcac caacaaaaga caaaccattc aatcgttctt
     1441 atacagtaga gatgctagga aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg
     1501 atatggaaac tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa tctgtttggt
     1561 ttggttgtga tgtaggtcaa tcttctacta gaactagtgg catcatggcc ttagatgctt
     1621 attcaatgga tgatctattt gatacggatt ttacaatgac taaagctgaa cgtttagatt
     1681 ttggagaaag tctgatgacc catgcgatgg tattgaccgg tgttgactta gtagacgggc
     1741 aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa agttggaaag aatggcttct
     1801 ttgttatgag tgatgcctgg atggacgaat atacgtacca aatcgttgta agaaaagatt
     1861 tattgacgaa agagcaatta aatgcgttca atgaagaacc aattgttcta tctccgtggg
     1921 atccaatggg tgctttagca tagacgagta tttcaatgaa gagtatgtag taaagataaa
     1981 aagagctggc ttaagccagc tctttttatc tttaaagtcc attccctgtt ttttatggtc
     2041 cctgtgctat aattctaaat aaaggataag gattggaaat aatcctttta tcattaaaaa
     2101 tatgatgacg aagtagaata aaattgggag taggggggaa agtcatggat atccaaactg
     2161 ttttatctga tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc
     2221 agacctatcg tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa
     2281 aaaaaggatt ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg
     2341 taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga
     2401 tcgagccggg aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc
     2461 aaacagcgcc acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa
     2521 attcttttga agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt
     2581 ctttggcaga agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta
     2641 aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag
     2701 acctgtggag tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag
     2761 ctgtttctta tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc
     2821 gaccggaatt tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc
     2881 gactgccgat ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt
     2941 acggttccca agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa
     3001 aaaaatatac caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga
     3061 catttccttc gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg
     3121 gaagaggcag tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca
     3181 tggagattaa tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg
     3241 agtacaataa gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat
     3301 tagtcaaaat agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg
     3361 ttaatgaaga gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg
     3421 aaaaaattga aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata
     3481 tcgatagtaa tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag
     3541 gatatgactt tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag
     3601 tagagtacgg gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca
     3661 aaacaaaaca tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga
     3721 aaaataaata aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt
     3781 tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt
     3841 ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa
     3901 cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc
     3961 tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc
     4021 gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac
     4081 agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc
     4141 cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa
     4201 gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat
     4261 gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa
     4321 gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt
     4381 cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc
     4441 ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag
     4501 ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt
     4561 atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga
     4621 tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat
     4681 gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat
     4741 tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat
     4801 gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt ttcattttac
     4861 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     4921 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     4981 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     5041 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     5101 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     5161 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     5221 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     5281 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     5341 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     5401 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     5461 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     5521 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     5581 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     5641 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     5701 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     5761 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     5821 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     5881 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     5941 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     6001 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     6061 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     6121 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     6181 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     6241 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     6301 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     6361 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     6421 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     6481 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     6541 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     6601 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     6661 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     6721 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     6781 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     6841 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     6901 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     6961 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     7021 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     7081 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     7141 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     7201 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     7261 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     7321 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     7381 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     7441 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     7501 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     7561 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     7621 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     7681 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     7741 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     7801 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     7861 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     7921 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     7981 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     8041 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     8101 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
     8161 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
     8221 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
     8281 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
     8341 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
     8401 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
     8461 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
     8521 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
     8581 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
     8641 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
     8701 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
     8761 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
     8821 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
     8881 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
     8941 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
     9001 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
     9061 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
     9121 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
     9181 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
     9241 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
     9301 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
     9361 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
     9421 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
     9481 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
     9541 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
     9601 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
     9661 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
     9721 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
     9781 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
     9841 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
     9901 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
     9961 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    10021 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    10081 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    10141 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    10201 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    10261 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    10321 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    10381 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    10441 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    10501 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    10561 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    10621 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    10681 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    10741 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    10801 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    10861 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    10921 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    10981 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    11041 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    11101 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    11161 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    11221 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    11281 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    11341 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    11401 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    11461 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    11521 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    11581 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    11641 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    11701 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    11761 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    11821 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    11881 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    11941 ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa
    12001 ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa
    12061 atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt
    12121 gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc
    12181 gtgtatcaga ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    12241 atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca
    12301 ccgttttatt tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa
    12361 acatgttaca ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca
    12421 gatacactgc caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg
    12481 ctggatgact caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt
    12541 ccttctgcaa cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat
    12601 ggctatgatg tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa
    12661 aaacgaatcg cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt
    12721 agaagaaatg aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat
    12781 cgagtaattg atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca
    12841 aaaaatgtga cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat
    12901 gaaattgtca aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc
    12961 ccagcttatg tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac
    13021 tttgatgaga attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt
    13081 gcgagaactc gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca
    13141 cgaccacttg ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga
    13201 ggagtaactg gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac
    13261 aaccagtgaa ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg
    13321 atcgttaact cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt
    13381 aaatcatacg tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa
    13441 acagcatgac ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg
    13501 gacgttatca gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa
    13561 aaccttattc aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg
    13621 cttgggatac gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata
    13681 ctttatggct gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga
    13741 agcttagaga gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag
    13801 tgtgggaatg gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct
    13861 tatctttaga ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac
    13921 gtaaagtttt tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca
    13981 actgggatcc tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg
    14041 aaggcgcctt ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg
    14101 cgacaacccg tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg
    14161 agcgttatca agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc
    14221 caatcattgc agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc
    14281 cagcacatga tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg
    14341 ttatggatga tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt
    14401 ttgctgctcg taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg
    14461 atacgatgat ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc
    14521 tgtctactca atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag
    14581 aaacagatca agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga
    14641 tggaaaatgt ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg
    14701 catggtatca caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg
    14761 aaaactgggt acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt
    14821 tctcaacaat gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta
    14881 gcactttagt aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa
    14941 gcttggaatt tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg
    15001 acgaacaagg gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa
    15061 tcgaaaagta tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac
    15121 aagatgtccg cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct
    15181 ggaatgcttc acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt
    15241 tatctggaga aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg
    15301 aacgagtaac tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata
    15361 actttatctg ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg
    15421 gagatgatgc tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga
    15481 tcctacgttt attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc
    15541 ctcatcaggg aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg
    15601 atgaaacagc ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata
    15661 ttcgttcaga agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg
    15721 atgctaaaat cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc
    15781 ccgaagagtt gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc
    15841 tgacaggtgc agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca
    15901 aacgattaga aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac
    15961 tttctaatga acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa
    16021 aagaaaaaga ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta
    16081 ctattcaata agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc
    16141 tgattgaaca tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga
    16201 gatgtgacat aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg
    16261 agggaggtgg atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct
    16321 gtttcttttc tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa
    16381 gagaaatggg ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat
    16441 taccatttgg ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc
    16501 atccagagag aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg
    16561 tagcttattt gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac
    16621 cttatatcga aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg
    16681 atttgatcca gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag
    16741 cagtagctgg tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga
    16801 tgaagaaagt ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca
    16861 atgtagctgt accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac
    16921 ttggagatac aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc
    16981 cagtagtaac aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag
    17041 caaaaaatgc accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg
    17101 atgagatgtg gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa
    17161 ccactccgct tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt
    17221 ttgtttactg tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga
    17281 agaaatctcg atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag
    17341 atggtgccca taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca
    17401 aaggatatca aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc
    17461 tagaacgatt gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa
    17521 aagccatcga attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat
    17581 tgtggcaatt cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg
    17641 tgaccggttc actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag
    17701 atcatgaagt ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta
    17761 gtgtattacg aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac
    17821 ctgtatttaa aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt
    17881 ttctcggaat ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg
    17941 cttgaacgtt tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct
    18001 tttttagaag aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt
    18061 gaacttttat tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa
    18121 aatgttcaac gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt
    18181 acagcgaaga aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct
    18241 gcggctgaga tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc
    18301 aaaaaaacag ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa
    18361 tccatctctt ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa
    18421 cgattgatca aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc
    18481 gcggaagctt tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct
    18541 tatagtgtga tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta
    18601 cgacaggcta ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc
    18661 gaaatcaaag ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct
    18721 ccaccggttc gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac
    18781 aaacaagaac atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa
    18841 acagtgttca tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat
    18901 gctgtacgtt attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt
    18961 ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga
    19021 tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa
    19081 gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta
    19141 tttattacta gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt
    19201 agataatact ggtaagtctc ctaataccat taaagcatac tgctatcatt taaagttgct
    19261 gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc
    19321 agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa
    19381 aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct
    19441 tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg
    19501 aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt
    19561 aaagttaagg gttaaaaaga aacagataag aacattgaga tcagaggaag ttaagcaaat
    19621 tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg
    19681 tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca
    19741 aatccattta acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga
    19801 aagaacaata catgtgagta aagaacttat gtcactttat acagattact tgatatatga
    19861 gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg
    19921 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
    19981 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
    20041 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
    20101 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
    20161 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
    20221 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
    20281 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
    20341 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
    20401 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
    20461 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
    20521 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
    20581 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
    20641 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
    20701 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
    20761 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
    20821 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacat ggatagcttt
    20881 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
    20941 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
    21001 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
    21061 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
    21121 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
    21181 ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg
    21241 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
    21301 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
    21361 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
    21421 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
    21481 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
    21541 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
    21601 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
    21661 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
    21721 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
    21781 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
    21841 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
    21901 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
    21961 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
    22021 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
    22081 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
    22141 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
    22201 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
    22261 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
    22321 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
    22381 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
    22441 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
    22501 gtcagcttta ttgaacagtc atttaggtat atgtccaatc tagggtaagt aaattgagta
    22561 tcaatataaa ctttatatga acataatcag ttacgaggtg aaatcatgag caatttgatt
    22621 aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga
    22681 agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac
    22741 agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa
    22801 ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc tgttagacca
    22861 cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga
    22921 gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca
    22981 agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta
    23041 tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca
    23101 attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt
    23161 ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc
    23221 gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact ggatatagct
    23281 agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa
    23341 gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt
    23401 gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt
    23461 gggaagaata aagcgtcctc ttgtgaaatt agagaacgct ttattacttt aatttagtga
    23521 aacaatttgt aactattgaa aatagaaaga aattgttcct tcgatagttt attaatatta
    23581 gtgacatttg catgcttcaa agcctgtcgg aattggtttt tagtgaaaag aacacgatat
    23641 tcacggttta cccacttata aacaaaagat cgatactttt tgtagtcctt ctttgaaatc
    23701 aatggttgat gtcgttcaag aacaatcaat acagagtcta cacttggctt aggatgaaaa
    23761 tatagtggtg gtactttttt gagcattttt atatccatct ccaccattaa tagtaaaccc
    23821 aaagctcgtt gcagattttg caatcttttc gcaaatccct tctcaacgat aagatagcta
    23881 tatttagcct gactttcaaa ggtaattctt ttgacaatat ccgtactgat gttataagga
    23941 atattaccat atatcttata gtttatatgt tttgggaagg aaaattttag aatatccgtt
    24001 tgaatcactt ttatattctc agaggggttt accgcttctt tagtcacttg acataagcct
    24061 ccatcaattt ctatagcagt aactgatcga ctcattttga ctagctcttt ggtaaaatgt
    24121 ccttttcctg atccgatttc tattacgttg tcttgtttac tgatattcgt gtgattcaat
    24181 atttctttta catgcttttt agaagtaata aaattttgcg tgtctttagg gtttttctgg
    24241 ttcattataa ccttctcctt actggttata atgaactgac attaagcgtt cattataacc
    24301 aattaatttt gatttggttg ataacgaact ttattaataa caaatataga aaaagtaccc
    24361 atacttttat ctcctttcca tttttttcat tatgaatgag ataaagtaat ttctactact
    24421 gcgatactgg tgcacataat tgaaacctcc ttatgtaact gaatatgatt gtatcatctt
    24481 ttcattttag aataaacctt tatatgtaat tttgcacttg acacttcaga tggacgtttt
    24541 cttcaaaata atatgttttg aaacttgata agagaagcca ctgtctttac taaaaagtaa
    24601 aggctgcgat aaaggacagt gactttaata aagttgcact gttgtattgt ctttggataa
    24661 aattgtctaa agggaagttt tatccccaac gaaaaaacat ctaaagtggt aagtaatgaa
    24721 agaatgtcga taaagtagga atgggggatc tttttgaata caatagaatc acagtttgat
    24781 aaggttgcag aagattacga tttcgtgaat gagcttttga atgattattc attctttgtg
    24841 tctaatatgt ctccaaagaa aggcagagca ttagatatcg gatgtggctc gggtttgtta
    24901 gtggagaaat tagcaagtta ttatgatgaa gtggtaggga ttgatatttc taatcaaatg
    24961 ctcgatcttg ccaaatctaa acgtcaacta acaaatacgg tctatctgaa tatgaatgca
    25021 gaacaactta attttaatga gaagtttgat tttattgtaa gccgaacaac ctttcatcat
    25081 ttggatgata tagccagcgt aatacagcaa atgaaggaac tgttgaatga ggaaggaaga
    25141 atagttattc ttgataatgt atctgaagtc gagacaccgc ctacctatgt ttataaatta
    25201 ggggcaatcc aagagttttt accgcattgt ttcaaatttg gaataaaaaa tgcgattaga
    25261 atttataatc acaacacatc taaatcatgg cttgagcatt tagcttcaga taagtaccta
    25321 tctgaacaaa attattacga tttatatgaa aagttgttgc ctggatgcca attccataaa
    25381 atgggttggg caatgggggt tgtctggaca aaataactta aagctgtggc tttgaaaagt
    25441 tgaatttgtg attgttttca gagctatcgg gtgctttagt ataaataaca atgaataaaa
    25501 aatcagcctg tatcttatgt tactattaag taatttaaga tacaggtttt actatttttg
    25561 aaataagtac cttccacttc aaatacatgt attcaaagtg aaagtattga tttaaatgat
    25621 tcttttgtaa tttactcttg ataatatgaa attccatcgc agcaggatat gaattttacc
    25681 aaaaggatac aaaagtgtgg agaaatgatg gggatcactg tactggacca cttgatcatc
    25741 ggacgcaaac gatattttag tttgagagaa gaaggaatga tggaagagaa gtaagttgct
    25801 ttcttgcaaa aaaaatgaag caagtgtaca attatattgt aattttgttt gggaatagaa
    25861 gttgaaacat ctgttgcacc tctatcgtac acgagcaatg ttacaattta aattagtgct
    25921 tttggcacga ggaggatatt attatggaaa acggaacagt aaaatggttc aacgctgaaa
    25981 aaggatttgg atttatctca cgtgaagatg gaagcgacgt attcgttcac ttctcagcta
    26041 tccaaggtga cggattcaaa acattagaag aaggtcaagc agtgactttc gacgttgaag
    26101 aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc ttaattttat ctgtaaatca
    26161 aattgatggt agacatcaat ttttacgaga gaacaaagcg agtaacaaaa atacctgcca
    26221 caagagacaa tttctcttgt ggcaggta
//
LOCUS       DACDCV010000029        25642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDCV010000029 DACDCV010000000
VERSION     DACDCV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.029"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..485
                     /locus_tag="IV041_001213"
     CDS             63..485
                     /locus_tag="IV041_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8382019.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(651..1844)
                     /locus_tag="IV041_001214"
     CDS             complement(651..1844)
                     /locus_tag="IV041_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8382020.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2068..2445
                     /locus_tag="IV041_001215"
     CDS             2068..2445
                     /locus_tag="IV041_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8382021.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2433..2771
                     /locus_tag="IV041_001216"
                     /pseudo
     CDS             2433..2771
                     /locus_tag="IV041_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2905..3348
                     /locus_tag="IV041_001217"
     CDS             2905..3348
                     /locus_tag="IV041_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8382022.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3609..4424
                     /locus_tag="IV041_001218"
     CDS             3609..4424
                     /locus_tag="IV041_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382023.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4434..5096
                     /locus_tag="IV041_001219"
     CDS             4434..5096
                     /locus_tag="IV041_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8382024.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5304..6968
                     /gene="alsS"
                     /locus_tag="IV041_001220"
     CDS             5304..6968
                     /gene="alsS"
                     /locus_tag="IV041_001220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8382025.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6981..7691
                     /gene="budA"
                     /locus_tag="IV041_001221"
     CDS             6981..7691
                     /gene="budA"
                     /locus_tag="IV041_001221"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8382026.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7821..8318
                     /locus_tag="IV041_001222"
     CDS             7821..8318
                     /locus_tag="IV041_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8382027.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8503..8763
                     /locus_tag="IV041_001223"
     CDS             8503..8763
                     /locus_tag="IV041_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382028.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9125..9364)
                     /locus_tag="IV041_001224"
     CDS             complement(9125..9364)
                     /locus_tag="IV041_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382029.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9650..9964
                     /locus_tag="IV041_001225"
     CDS             9650..9964
                     /locus_tag="IV041_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382030.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10398..10764)
                     /gene="ssrA"
                     /locus_tag="IV041_001226"
     tmRNA           complement(10398..10764)
                     /gene="ssrA"
                     /locus_tag="IV041_001226"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10935..11708)
                     /locus_tag="IV041_001227"
     CDS             complement(10935..11708)
                     /locus_tag="IV041_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8382031.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11852..12202
                     /locus_tag="IV041_001228"
     CDS             11852..12202
                     /locus_tag="IV041_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8382032.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12183..12899
                     /locus_tag="IV041_001229"
     CDS             12183..12899
                     /locus_tag="IV041_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382033.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12899..14347
                     /locus_tag="IV041_001230"
     CDS             12899..14347
                     /locus_tag="IV041_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8382034.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14417..14926)
                     /locus_tag="IV041_001231"
     CDS             complement(14417..14926)
                     /locus_tag="IV041_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382035.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(14916..15641)
                     /locus_tag="IV041_001232"
     CDS             complement(14916..15641)
                     /locus_tag="IV041_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382036.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15864..17984)
                     /locus_tag="IV041_001233"
     CDS             complement(15864..17984)
                     /locus_tag="IV041_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8382037.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18214..19392
                     /locus_tag="IV041_001234"
     CDS             18214..19392
                     /locus_tag="IV041_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8382038.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19408..20166
                     /gene="aroD"
                     /locus_tag="IV041_001235"
     CDS             19408..20166
                     /gene="aroD"
                     /locus_tag="IV041_001235"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8382039.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20189..20674
                     /gene="ybaK"
                     /locus_tag="IV041_001236"
     CDS             20189..20674
                     /gene="ybaK"
                     /locus_tag="IV041_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8382040.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20745..21998)
                     /locus_tag="IV041_001237"
     CDS             complement(20745..21998)
                     /locus_tag="IV041_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8382041.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22116..23465
                     /gene="gdhA"
                     /locus_tag="IV041_001238"
     CDS             22116..23465
                     /gene="gdhA"
                     /locus_tag="IV041_001238"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8382042.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23577..24923
                     /locus_tag="IV041_001239"
     CDS             23577..24923
                     /locus_tag="IV041_001239"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8382043.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25231..25617)
                     /locus_tag="IV041_001240"
     CDS             complement(25231..25617)
                     /locus_tag="IV041_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8382044.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         8480 a         4420 c         5122 g         7620 t
ORIGIN      
        1 gatatttgta tccgtttttt tcttgtatgt agtatagtaa agtagataca ggaggagaaa
       61 atatgcagac ttttttaaca aaagtttctc aaaagaaaat taaaatgatt catttactgc
      121 ttgaaacaga acgttggtgt tcaatagaag aactacaaaa cgaattgaag gtatcaagta
      181 agtctatctt gaattatcta acagatttag aagaattgtt ccaacaatat cctgataagg
      241 tcattttgaa gaatgagaat aatcggcgct tttctatgga aaagcaggag aacttcccaa
      301 tctacattat ttatcttcat ttctacagaa aatcatataa ttatcatttg attgaattca
      361 tgtatcaaca tcctgagaaa aatttggaag attatgcaga cgctcaatat acgagtgtat
      421 ccacagtttt tcgttatgct aagttattag taaagtactt tgaaatgacg gacgtcaaag
      481 aatagtcgcc gccagataga aggaaattag cagttgaaaa agctgccctg catcaagtaa
      541 tacgaccagc caaacggaaa cagtgtctca tgtttttgat ttggctggtc ttattaccgc
      601 agatgcaagc tttttccaca cctctttctt gtcatttaca gactcaacta tcaatgctgc
      661 aagctttccc ttaatgctgt aacaccagta agagttgtca agaccagcag actgactagg
      721 taaataccgc tcaaaaacaa gattacggca gttaaactat aatgatccat caagaaaccg
      781 atgacgatag aagaaaaacc accaacagct cgtccaaggt tcataacgaa attattcgcc
      841 gtcgcgcgga tttctgttgg atacaaacta ctaatcactg cgccatatcc accatacatg
      901 ccattgataa agtagccgac tatcacagcg gctgctacca aactccactg gctatgggct
      961 aacacaagta gatagacaga taaggatgaa caaattaaaa acaaagaaaa ggctagttta
     1021 ggccccaatt tatccatgat tatgccaaaa gtcatcatcc ccaaagacat gcctaaaata
     1081 gtactgacca tccacaatga agacccagaa accgaaagcc ctaatcgatt ttgcataatt
     1141 gaaggtagcc aattcattaa cccaaaatag ccggcaattt ggacagttac catgatacta
     1201 aggccacatg tttgccaagc aagatgtttt gattcaaaca gtctgctaag tttggttttc
     1261 ttttcggcag attgtacaga tttgaattcc tttggttcag tcaagccgcg acgaatgaac
     1321 aagaccatca acaccggcag tacacctacg acatacaaca tcttccaacc aaacgttgga
     1381 atgatcacag cagctaaaat cgcagccaaa gccgcaccga cctgcgcacc aataccagca
     1441 accgaagaag ctcgtccaat ttgttttttt gaaaaacttt cggaaatcaa ggacatacac
     1501 gccccatatt ctcctcccgc tcctacacct gcgataaagc gaaagagata aaccaagtag
     1561 atgtttgaag caaaatacat caataaagaa gcaatcgaaa acagaaggac cgtttgagaa
     1621 aatactttca cacgtccata tttatcagct atgatgccaa aaaaaatacc tccaatgagc
     1681 atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg taccactaat atgaaaagaa
     1741 gtaatcattg aagataatga aaaagctaaa aacataatat ccatattctc taaagcaatt
     1801 cccgccgatg tcgatactaa tacctttcgc tgatacgttg tcatttttat accccttttc
     1861 ctaaaatttc cctaaattgt taaaagtata tccaaaaaag aataaaaaaa caagactttt
     1921 ttatttgaaa taatacacaa acaaccgata tagataatcc agcttagttt taatagaaat
     1981 gagtattgac ttggagtaca cttaaagttg tagaataaat aaaatacata agtaaagtgg
     2041 caagaaatta agagaaagag tgatcagatg aatatcaaac aagtcagtga agaaaagggc
     2101 atttcggccg atacattgcg ctattatgaa cgaatcgggt tgattccacc agttaatcgg
     2161 acaaaaggcg gaatccgtga ttatacagaa gaagacctaa aatgggtcga ctttacgatc
     2221 tgtatgcgaa atgccggatt atcgatcgag tctttgattg aatatattcg cctatctagc
     2281 gaaggagaag caactgtatt tgagcggcgt aagcttttga ttgaagaaag tgaacttttg
     2341 acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt tacaaggaaa aatcaagaaa
     2401 tatgatcgtg tgttatctag aaataaaatg gaatgtatca agtaaaaaaa tcaaggagca
     2461 tcgctatgga gttacgctta ctaaaatatt tttggacaat tgcagaagaa ggaacaatct
     2521 ctcaagccgc agaagttcta catcttacac aaccaacctt aagccgacaa ctgaaggagt
     2581 tagaagagta actaggaaca gaactgtttg tacgcgaaaa ccgcaaaatg atcttgacag
     2641 aagcaggtta ttttctaaaa aagccgagca gaagaaattt tagatttaac gaatcaaacc
     2701 actcaagaat tcgagaacag aaaaaaacaa ttattcagcg gccgatttac gatcggctgt
     2761 gtggaagctg atagttcgaa tacattggca ctattactag aaaagttgat ccgtgattat
     2821 cccagcgttc aatttcatat ccacagcgga acaagtgatg atatcacgga gtgactagaa
     2881 aaaagcctgt tagatttagc gattttgcta gatccagtag atacagagaa atacgaaacg
     2941 attttgttac caagaaagga aaaatgggga ttgctcattt tagaaaattc tgttttagca
     3001 aaaaaagacg tgataacaaa agaagacttt gcgaatgtac ctcttttgat ctcaaagcgt
     3061 ccagaaatac agcgaatgtt tgctgactgg ttaggcataa aaaaggagca gttgctgatc
     3121 acaggaacgt acaatttgat tttcaatgta tttgcgctaa ttgagaatca ggtaggtgct
     3181 gctttagtca tagaaggtgc tgtaagtcat cattcaaagc gagtgacatt cgttccgctt
     3241 gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa agaatcggat tttatcacct
     3301 gtagtcaatg aattgattca ccgtttcaaa gaagcttttg aacagtaata agtatctcta
     3361 atcgaagaaa tttcaataca aaagcgagac gtatgacagt cttgcttttg tattttttca
     3421 tttttaaatc aaaaaaataa agagatttac ataacttgtt aaataagcgc ttacaaaaat
     3481 aagaagaacg gaaatgaatc agctataata accgttgttc tggattgtaa tcattacaaa
     3541 gaaatgaaga ttataacttt ggaggtgttc gaaatgaaag aaaaaaagca aaaattcgtt
     3601 caaaaaacat gccctttatt caatggttgg gtatgattct gctgattgta ttagctttat
     3661 tgacgatcat tcctttttta tggatggttt ctgcttcatt caaagcaaat aatgaagtgt
     3721 tcacgattcc gattcaatgg atccctaaaa cttggcattt agagaattat ctggttattt
     3781 ggtctcgtat tcctttagtg acatttttta agaatacagc ttttttaagc gttgtgatca
     3841 cgttgattca gttgtttact tctagttttg ctgcttacgg gttttctaaa ctaaatttta
     3901 aagggcgcga tattttattt ttagcatata tcggcacgat tgctgtccct tggcagtctt
     3961 atatgatccc gcagttcatt atgatgagaa aagtgggttt aacagatacg ttgtggtcgt
     4021 tggtgctatt acaagcgttc aatgcttttg gtgtgttctt gttgaagcaa tattacagta
     4081 gtatcccaga ttctttatgt gagagtgcgc ggatcgatgg cttatcagag tggggaatct
     4141 atcgcaagat cattttacct cttacaaaac cggcgattgc cagtttgacg atcatcacat
     4201 tcgttaatac atggaacgat tatatggggc cattcattta tttgtcttct acggaaaata
     4261 aaacaatcca gttagggttg aaaatgttta ttgggctatt tgatgcagag tacgcattaa
     4321 tcatggctgc ctcagttgtt tctgttcttc ctgttgcttt tatattttta gcgatgcaaa
     4381 aatattttgt agaagggatc gcttcatcag gaatgaagga ataggaggtg attatgaaag
     4441 agcagcgttt tgacaaaaat gtttatatgg aattctttta ttggttgtat caatcattgg
     4501 caagtagcct ttgtttttgg tgtgtcaata tcttgtatat agcagctgcg ttccttttgt
     4561 cgatagatgg aaggaatgtc ttttccttta ttttttcttt actttttatt ggtccaggtt
     4621 gcatcgtgat gataggtatg acagaagaat tagcaaaaga aaatagtttt tcaccagtac
     4681 gtttgttcat aaagatgttt cgccaatttt ggctaaaggg atttttttat tggctatttg
     4741 cttggatagt tagtgtgata atgatttttg attgtttctt ttttatacgt ttttcttatg
     4801 gaaaatggtt gattccgttg tttgttttac ttgcttgttt atcagtttcc tttagcacca
     4861 actgctggta ttttcaggtg agaaatccag cgtcgaaacc aaatcaagtt ctacggatag
     4921 cgttctacta tacgttgaaa aaatggtatg tgagtttact agatttttta ctattgacaa
     4981 gtttgtttct cttctttttc gtaaagcccc aatggtgtat tctactaggt ccttctatcg
     5041 tatttggttt gatttacttc aacaatcgta aattgatgag aactatggat ctgtagactt
     5101 tataaattga aactttcaat taagaatgac agtttttttt atgtaaaaac tgtcattctt
     5161 tttcattttt tgaaacatat ggctaaacaa atgaaataaa acgtgctata cttttgtatg
     5221 aaacaggttt agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata
     5281 aagcataaaa aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag
     5341 tagaaagttt gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg
     5401 atggtgtatt taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac
     5461 aaaatgctgc atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta
     5521 tcgcaacaag tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag
     5581 aaggtgaccc agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga
     5641 cccatcaaag catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa
     5701 tccaagatcc agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt
     5761 caaaaaaagg cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag
     5821 gtaatgtcat caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc
     5881 gttacctagc tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag
     5941 gatcaagtga aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg
     6001 ttgtagagac attccaagca gctggagtga tctcacgtga attagaagcg catttctttg
     6061 gtcgtgtggg tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag
     6121 ttattgcaat tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag
     6181 atgcgcggat cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg
     6241 aaagagaatt gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc
     6301 ctcaacaagt gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg
     6361 agcgggacat tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta
     6421 acacgcttca gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt
     6481 atatttggat ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg
     6541 gtatgcaaac gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa
     6601 atacacaaat cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag
     6661 aaacggcggt acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca
     6721 atatggtaga gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc
     6781 cagttgattt tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg
     6841 tagaggaatt agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg
     6901 atattccgat tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat
     6961 tctattaagg aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag
     7021 ctttgatggc cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa
     7081 ctctgggatt gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag
     7141 cttatcaagg aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct
     7201 atgcagcaat tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg
     7261 gagaacaatt gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag
     7321 tgaagataac tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac
     7381 cttacagaag actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatatcc
     7441 aaggaacatt agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat
     7501 ttcacctgca ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag
     7561 tggctgaagg aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg
     7621 atgatccgac ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac
     7681 aagcagaata gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt
     7741 tttttgagga ggaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc
     7801 aaaaaggagc ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt
     7861 tattattaca gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa
     7921 attcatctgc attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat
     7981 tcgatggtaa tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca
     8041 agatgggaaa aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg
     8101 atgtcaaaac ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag
     8161 tgccaatggt aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga
     8221 aagggtatga tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa
     8281 attttaatat aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa
     8341 gaagagaaat caagaataaa aagagccgga aagacagtgg tcatttctgt ttttcggttt
     8401 ttttattttt ttataaaatc acaagtgtta ttgattttca caatcatatt ttaatggtat
     8461 tatcattttg aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc
     8521 attttaggaa aaaaatatat ttgccaacct attggcttga aacaaaaagt ggttggagaa
     8581 gtaatcagta aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat
     8641 cgcaatgaaa tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag
     8701 ttggaaacaa cagaatatct atataactat agcaacaaat atgaacatac tttagtttca
     8761 taaagattta aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat
     8821 ttagttggca gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct
     8881 gtctctgtaa cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc
     8941 tattttcgga tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa
     9001 aatgcaaaac tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa
     9061 ggttgcattt agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa
     9121 gaagctaatt cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt
     9181 tttttgcaga ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg
     9241 tagaaattgt tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg
     9301 cagaagtccc acaagcagta agtaacaata aatttgataa actgataaac agaattcttt
     9361 tcattatgta cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca
     9421 aaatcaatgc aaaactatca agtcttttat agtatctatt agtcttagta atacatatat
     9481 ggagagggtg atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta
     9541 tgcctctaaa cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat
     9601 ctagaggtaa tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta
     9661 cagtaatttt atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa
     9721 gagtagaaat gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg
     9781 ggtatattca cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac
     9841 actttattct tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat
     9901 ccttatttct gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa
     9961 ttaaagcaat tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg
    10021 tgaagcctgc tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta
    10081 ggatgaatta atttctctag agtttataat taaaaaactt atttgaagga acatgcttaa
    10141 accataattg tgtttttcct tccatcagca taaaagttca cctcaaaaag agtgtacgaa
    10201 caaaagttct ttttgtaaac cttaaaatag attttcctga atacgtattg gggatttcta
    10261 aatgtttgaa tgtttttttg aatgcaaaat caatgcaaaa aattttgtat ttctctactt
    10321 ttctctcctt gaccaaaata aaaaaacgtt gatttaacaa cgtttttcat acatgtttat
    10381 ctgtctcaac catacatata tggagacggc gggagtcgaa cccgcgtcca aacacatcgc
    10441 cacttaaaaa tctacgttca tagtcaactc atttaaggat tcactttaca gcttgccgag
    10501 tgacaggcat tctgtatcgc tagtctgatg ttctcttttt caacttacag acggaaagtt
    10561 gaaacgtatc ccactaaagt taggaccctt actcgagcac atgggcgatg ccgagaggat
    10621 ctacgctaac tgtttttagg cagctaaagc gtaagagttg ttttcgtttt tagcagttat
    10681 atttaactgt aacgttttta cgtagacgta acctacgaaa cgcaattcaa gctcgactgt
    10741 gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt tacgtgtatt tttcatacaa
    10801 aattagtata gcatatattg ggataaattc aataaaaaag cgaatcatat cccattattt
    10861 atataataac tgcccctttc ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc
    10921 ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg cgtggacaat tccgccttct
    10981 gtatttttct ttgtgataaa ggtagaaagt tcttttaatc cgtcataggc gttgcccata
    11041 gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat tcggaccatc gccaaatcca
    11101 aaagtaggaa ctttttccag ctgaagttct ttttgcagac gtttgacacc tgcccctttt
    11161 gagattcctt tcttcactgt atcgatcgag taaggaccat tccgataaaa agttagctca
    11221 ggaaaatgtt cgtgatagta ttcgtcacct gattgagata agatcagcaa catatttact
    11281 gtgttttctt taaagcctaa aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa
    11341 gcattgatca ttgtttggct atgaccggta caccagattt tctgatcgtt atagtaagaa
    11401 agttcatgac cgttcaactg tacatgatca tacatttttt gacattcttc tgaagaaaat
    11461 gtttgagaat agatttcttc gccgtctact cgaatgaaag aaccattcat ggtgaccgca
    11521 gaagtaatgc ccgcatcttc catgatagag tggatttctg cttctgtacg tcctgtagcg
    11581 atgacgggga gaacattgtt ttcttttaat gcgttcatag cttttgccac ttcgggtgtg
    11641 atttttgatt tcgcatctag taatgttcca tcaagatcaa agaaagtgat tgcacgataa
    11701 tttgacacaa ataaaactcc ttttttattg gaataagttt atcttaacaa agaatgaaca
    11761 aaagacaaac ctttcgtgaa gaattcctga acatattggt tcagtcaaca atttagagta
    11821 tactataaga agagaattta ggagggaaag aatgattttt ttcagtattg gtatcttatt
    11881 cttaataatt ggcagtcttt ttcggattct gccctcaaaa ggaaatctgc ctttttatgg
    11941 gtatcattcg cctcttgccg caaaaacaga cgctcattgg cggctggctc aaaagacaag
    12001 cggaaactgg ttttttctta tgggcttact gatggcattg atcggctatt atttaaagac
    12061 atctgggcat acgaattatt ttctgattga aatgcttctg ctagtatttc caattatgcc
    12121 tatttttatt atgacagaaa agaaattaca gaaatatgac ctggaaactg gagggaacga
    12181 taatgaatat tttaatgatt gaagataatc aatctgtttc tgaaatgatg cagatgtttt
    12241 tcttaaatga aggttgggaa gcaactttca agtatgatgg aaaagaaggg ctcgatgcct
    12301 ttttagaaaa cccacaacat tgggatatga ttacactaga cctgaatttg cccacactgg
    12361 acggtatgct agtaggaaga gagatccgca aagtttcaag tactgtgccg attatcatgc
    12421 tgacagctag agattctgaa agcgatcagg tgatcggctt agagatgggg gcagatgatt
    12481 atgtcacaaa acctttcagt cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc
    12541 gtagtgagtt ggcagaagca agagaagaag cagcagtttc agatgatcat tttgatatcg
    12601 agactagtca tttcaagatg aatacgaaaa cacgagaagc gtatctggac aaccggccaa
    12661 ttgaaggact gacgcctaaa gagttcgatc tattgtttac tttagcaaaa aaaccacgtc
    12721 aagtattctc tcgggagcag ttgcttgaat tagtctggga ttatcagtac tttggtgatg
    12781 aacggacagt ggacgcccat atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac
    12841 aagtcatcca aactgtttgg ggagtcggct acaaattcga tgattctggt gttgcctaat
    12901 gcgttatttg tatcagcaac tcttagcttt ttgcggaatg gtcgcgttga tcttattgat
    12961 cgttggagtt tcttttacac aactgacaaa acaaacgatt gaagaaaaca attaccagca
    13021 actgtttgga tatgctgaat cagttgaaaa gacaacgcaa acctatgccg atacgctccc
    13081 gcagtttagt caagatcagg cgtttcaaaa tgcattgctg ctgacagagc aagtactgac
    13141 agaacaaaat gtgaacttta tatttatcga taaaaacgaa cgtgtcattt atccgactaa
    13201 tacagcttta ctgaatttta ccttaacagc tgatcaatgg aaaaatctga ggaatggacg
    13261 tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa gcacaggcga cctcttacgc
    13321 acttgtgcca tttaatttga atcatgaatt ctatggagca cttgtagtta gtcaacctgc
    13381 gcgaaatatc gataacagcg ttcgtccaat tacactgaac ctgttcaaag gatttatttt
    13441 ttccagtatc gttgcagtta ttgccagcta tgcttttgca gcattccaag ttaagcgtat
    13501 caatcggtta aggaatgcga cgaaagaagt gacgaatggt aattttgatg tacagctccc
    13561 cgtacatgac aaagatgaat ttgatgagtt agcagatgat ttcaataaaa tgacgaattc
    13621 attgaaagaa tctcaagcag aaattgaaga acaagaaaat cgtcgaagac aatttatggc
    13681 agatgcttct cacgagatga gaacacctct tacgacaatc aacggacttc tggaagggtt
    13741 agaatacaat gcaatccctg aaaatcagcg agaaaacgcg attaaattga tgaaaaatga
    13801 aacagagcgt ctgatccgtt tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa
    13861 tcaaatcagt atggtcatta aaaaattcaa tggaacagaa acattgaaaa atattgttgc
    13921 acagttagaa gctaaagcag aagcagcagg agatacattg acattgaaag ccgttgatga
    13981 tattgatgtt tacgctgatt atgaccgatt tgtccaaatc atggtcaata ttattcaaaa
    14041 cgcgatacag tttactgaaa acggccaaat cacgattact ttagaaaaag gctatcttga
    14101 aacgatcatc acgattgaag atacgggaat tggaatgtct gaacaacaaa tgaaaagtat
    14161 ctgggatcgt tattataaag tagatccttc aagaaaaaat acaaaatatg gtgaatcagg
    14221 attaggttta ccaatcgtgc agcaacttgt acgcttgcat aaaggaaaat tagaagtaga
    14281 aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa
    14341 agagtaaaaa acaagcggta taaaagatca tgaatcgatc ttctataccg cttgttttta
    14401 ctataaaatg attgatttag cccgcgggct ctaaacgatc aatataagga ctttgagcaa
    14461 tagtagcatc atgagtaaag agtaagatac cttgattttc ttcatgagct aaataagaaa
    14521 gcaagtggag tagctcttgg atctccaata cactaagttc atcaaatgta tcttcaataa
    14581 caatattgtt tcgcttggaa agcaaggcat gaagtaattg cagctttact ttttcaaaag
    14641 acgtcaactc agctgccgca caattaagca aggaaggaga gatatcaagt ttttcttgat
    14701 aggccaaacg atcttgtttg atttcttttt ccggtacatc caacaggaca tggtctttaa
    14761 gattcagata aggaaacatc ggccaatttt tttggatgat cgcaaaatta tccagttttg
    14821 caacaagaac ctcttttttc aagtgctcca atattttatg ctgtttttcg agaggacaca
    14881 aaataacggt caagtgttca gcatgaatca aatattcagg tttcattacg tccaggcctc
    14941 ctactttgca aatgatggat aaaaaaagtg aaagggaata gactaagaat gagacagcat
    15001 ccactgagcc caataagagt ttgggcaaat cccttaagta tatctgtgaa aaaaagatca
    15061 ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg gaattgtaag tccgcttacc
    15121 gaagtcaaga tatcacggat atctggcagt tcagtagtct ttaagacaag aaaattgatc
    15181 ctttgaagaa gcgcttcata aactgactgg aagcttacga tccacaataa aaccaagata
    15241 caacaaagaa agagaggaaa gaacagctgc cagaaaataa aaaggaataa tttggtttta
    15301 gaaagaccag tcaggcgcca attgatcaat tcctgcttgt tttgcctgac tgaatagtaa
    15361 aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac tgaatatgcc ttttatataa
    15421 aatcggctag ctaattctaa agccggaaga agctctgtag atgcagaagc gccaataaat
    15481 gaaagccgat cttggacttg attggctaaa gccgattgca aatggtaaag agtcaggcat
    15541 gctatcaata aaaaagcaga aaagcttgaa aataaacaat agagtattgt ctgctttttg
    15601 taataaatta atcgagcagt agcatagcgg attattttca tacttttcac ctctataaaa
    15661 agtatagaaa atgaatatga ataaagtatg aaattccttt agtgaaatca cgaattaaaa
    15721 ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt ctattaccgt
    15781 agatgcaagc ttttgaacac tgtttattcg aaaagaggag cagcgacaat aattttgtcg
    15841 ctgctccttt taaatttatt ttgtcattcg gaagaacttg tggtcgttcc gtgaagttct
    15901 tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc actaaagaca
    15961 ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa gccattcttg
    16021 tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa ccgaagaaga
    16081 tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc tttcagtgaa
    16141 tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt ttcgttacta
    16201 tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc acggaaagaa
    16261 acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata attttttgta
    16321 tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga
    16381 ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg ctgcatttgt
    16441 gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc tagattttta
    16501 tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga attatcatac
    16561 aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa ataattggct
    16621 gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg
    16681 tcattggtga attttgaata aggataatgg ttagatacag caatgaattt tgaataaaat
    16741 ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt atcatgcaac
    16801 ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa gaaataatca
    16861 tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt cccatggaat
    16921 accgcactag tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg gaaagtattt
    16981 tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga attttccatc
    17041 agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg
    17101 aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg ctggagacca
    17161 ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa atggatataa
    17221 atcacatttt ttcctttagc gagcccgaac aaatcgtcat ttggtgctgc ataatgttct
    17281 tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg cacttggttt
    17341 gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag atatttgacc
    17401 aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc cgccataaaa
    17461 agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat tcttgctcgg
    17521 atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa gataataaaa
    17581 tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc tcccagacca
    17641 ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga
    17701 taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac catcatcgtg
    17761 atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa caatgtagtt
    17821 gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc gagatggaaa
    17881 tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat
    17941 ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat cttcctttct
    18001 gcacttctta atcacagaag tgtcatgaaa ccatgttaca cagctcactt attctcattt
    18061 attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct gtttcattaa
    18121 ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt tttttgttat
    18181 acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca
    18241 agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc tcatccccaa
    18301 aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat cgcaaaagga
    18361 tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa catcccattt
    18421 gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac tttctttttt
    18481 tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg aatcggcggt
    18541 ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga aacgttatat
    18601 gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat cgtaggaggc
    18661 tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat ttacggaaaa
    18721 gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat
    18781 gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc attagcagaa
    18841 ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc cttttctgtt
    18901 gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa acgcagttta
    18961 aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt
    19021 gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt atatgacatg
    19081 attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa
    19141 gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg gatacttatt
    19201 gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat tgaagaggaa
    19261 ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc aaaagatttc
    19321 cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta tcaaattaaa
    19381 aataaccatt agaaggagcg atgcagaatg gtttgggtaa agggattgaa tataggaagc
    19441 ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt attacgtgaa
    19501 gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga tcattatcgt
    19561 gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga gatgcttgat
    19621 ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca agacatatcg
    19681 atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt agatatggtc
    19741 gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga aatcaagtct
    19801 ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc agaccctgtc
    19861 ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa actggcagta
    19921 actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg ggcaaatgct
    19981 tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg
    20041 gctggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga ttctacttct
    20101 gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt aaatgaaccg
    20161 ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc
    20221 aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg agtgggacga
    20281 aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat ctcgtatatt
    20341 taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga taccgggaga
    20401 agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag tagaaatgct
    20461 ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct cgcctatagg
    20521 tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg aacaaatcat
    20581 tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata tctgtgcatt
    20641 aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag aaagaaggag
    20701 gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc tctttgcccg
    20761 gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa gccgccagta
    20821 taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat tttactctcc
    20881 aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa tgatttctca
    20941 ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac aaaatcttga
    21001 atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag
    21061 aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt ctttttggtt
    21121 gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt agggaaacaa
    21181 tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac aaaacttgaa
    21241 caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc catcgtatgt
    21301 tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc
    21361 ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga ctctgtcgca
    21421 taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc tccagtagaa
    21481 ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc atggaactct
    21541 tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt ctctactcga
    21601 gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc ttcgataatc
    21661 gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt taaatgtacg
    21721 ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa cgtactatat
    21781 aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg attgttcgta
    21841 acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt gtttttttcg
    21901 atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat cccgcttgtt
    21961 ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca aggaaagtgt
    22021 agcatatctg aagaaagaaa acagaaaaaa aatgttttga gccaaaagag aaaatgagct
    22081 aaaatgaggg aagataagca aaggagcgtg gaacgatgac aaacgcaaca gaatatgttc
    22141 aagcaattca agaaaagcta catcaaaagg atcaagggca agaagaattt ttacaagcaa
    22201 tcgatgaatt catgcccacg gttatgtctt ttttggacac tcatcccgaa tatatcgaaa
    22261 agaatatctt agaactttta acggaaccag aaagagttat tcaatttcga gtgccttggc
    22321 aagatgatga aggaaactgg cgagtaaata gagggtaccg tatccaatac aactcagcta
    22381 tcggccctta taaaggtgga ttgcgcttcc atcctagtgt caatctaagt atcttgaaat
    22441 ttttggcttt tgaacagatt ttcaagaata gtttaaccgg tctgccaatc ggtggaggaa
    22501 aaggcggaag tgacttcgat cctaaaggaa aatctgataa tgagatcatg cgtttttgtc
    22561 aaagtttcat gctggaattg tcaaaacata tcggcccatc gattgatgtt cctgcgggag
    22621 atatcggtgt aggagctaga gaaatcggct atttgttcgg cgaatataaa cgattgaaaa
    22681 aatatgatac tggtgtgttg acaggtaaac ctttggattt ttggggaagt aagatacgta
    22741 cggaagctac tggttatgga ttagtgtatt atgtcaaaca tctattgaac gaagaaaaag
    22801 attcatttaa tcaaaaaaca gtttttgttt ccggaagcgg gaatgtagcc atttatgcaa
    22861 tcgaaaaagt gcaagagcta ggcgggaaag ttgttacatg ttcagattct agcgggtaca
    22921 tctatgatcc agaaggaata gatgtgaaac tattgaaaga agtcaaagaa atcaatagac
    22981 aacgtttgac ggaatacgca aaccaacgtc cttctgctat ttatcatgct ggagaatctg
    23041 tttggacatt gaaagaaaaa gcggatatcg ctttgccatg tgctactcaa aatgaaatag
    23101 acgaaagctt ggccgaaatc ttagtagaaa atgggatcaa aatcgtagca gaaggcgcta
    23161 atatgccatg tacagtaaaa gctgtttctg ttcttcatga agcaggcgtg tggtattgtc
    23221 caggaaaagc tgctaatgct ggaggagtag cagtatctgc tttagaaatg agtcagaacg
    23281 tccagcgatt gacttgggaa agagagcaag tggatgagca gctggatcaa atcatggaaa
    23341 cgatttacca tacgtgtcgc caaacagcag ccgagtacgg cgacgaaaaa aatctgcttc
    23401 ttggagcaaa tgttgctgga tttgaaaaag ttgccaaagc aatggcagca gaaggtttag
    23461 tttgaaaaat gaaactttct tggaaatatt gtcagcattt tgtttatttg cttgaaaaat
    23521 aataaaaaaa aacgtaaagt attacctgta gagtaaacct ataaggagga gaacaaatgg
    23581 cacacattca ttttgactat tcaaaagtag caccgtttgt caacgaacaa gagttagggt
    23641 acatgcaaag ccaagtaaca gcagcccaca acgaattacg ggaaggtact ggcgcaggaa
    23701 gcgacttccg cggctggatc gatttaccaa cgaactatga caaagaagaa tttgcacgta
    23761 tcaaagaagc agctaaaaaa atccaatctg attctgaagt attagttgtt atcggtatcg
    23821 gtggttctta tttaggcgct cgtgcagcga tcgacttctt gaatcacaca ttcttcaatt
    23881 tattagatag caacacaaga aaagcaccac aaatcttctt tgccggaaat tcaatcagct
    23941 caacatatat tgctgacttg atcgaagtaa tcggtgatcg cgacttttca gtaaacgtta
    24001 tttcaaaatc tggaacaact actgaaccag cgattgcgtt ccgtgtattc aaagagcttc
    24061 ttgttaagaa atatggacaa gaagaagcaa acaaacgtat ctacgctaca acagataaag
    24121 caaaaggtgc agttaaagta gaagcagatg cagaaggttg ggaaactttc gtgattcctg
    24181 atgacatcgg cggacgtttc tctgtattaa ctgctgttgg tttgttacca atcgctgtta
    24241 gtggtgcaga tatcgatgca ttgatgcaag gtgccgctga tgcaagcaaa gcatactcaa
    24301 gcgacaaact agaagaaaac gaagcttacc aatacgcagc aatgcgcaac attctttacc
    24361 gtaaaggcaa agtaactgag ttgttgatca actatgaacc aggaatgcaa tacttctcag
    24421 aatggtggaa acaattgtat ggtgaatctg aaggaaaaga ccaaaaaggc atctatcctt
    24481 caagcgcaaa cttctcaact gatttgcact ctttaggaca attcatccaa gaaggacgtc
    24541 gcaatatttt tgaaacagtt gtgaaggttg aaaaacctcg taaatcaatc acaattcctg
    24601 aacaagcaga agatttagac ggtctaggct acttgcaagg aaaagaagtg gacttcgtaa
    24661 atacaaaagc atttgaagga acattgcttg ctcatacaga cggtgacgtg cctaacttgc
    24721 tagtcaaaat tccagaaatg gatgcttata cattgggtta tacaatgtac ttctttgaaa
    24781 ttgctgtagg tatttcaggt tacttgaatg gaatcaaccc attcgaccaa ccaggtgtag
    24841 aagcttacaa gaaaaacatg ttcgcacttc taggcaaacc aggctttgaa gatcttgcta
    24901 aagaattgaa cgaacgcctg taagataagg gtttttcgct agatttaaat aatcagtgat
    24961 agaaacatgt tcgctttaaa atgaaaactg gtacccattt aggtactcaa acatactgca
    25021 tcgtttattc agaataaggg gctgtgacaa gacgtttgtc acagtccctt gtttgtatag
    25081 aaatcgattg attgccttgc tctttagttt gttttaggac tttaagaaca gtgaggctga
    25141 cttcgaaggt taattctgtt tttttgtttt ctagaatcag taagcaaaaa agcactcact
    25201 tcgagtgctt ttttgcgata tattcttagc ttaagatagg cctaattttt cttttacaga
    25261 tgagggtacc ttatcgctgg taattacttt tttctctatg atccgctttt cgcttgcttt
    25321 caactgcaag atcgttcctt cggggtaagc agaaccattg gcagagtcac cagaagtaat
    25381 gacatcgaaa tctaactgtc ttttttcacc gttttcagca taagcatcta cgttgtattt
    25441 aaaacctctt ccaattactt ccccattatc tgctttgata tcagtttctg caggcattgg
    25501 agctttgact accgcataat aatctttacc tacataagta gactgatagt agtcccaacc
    25561 tttccatcct ataaaagcaa tcaatatgat aacgatcaac cctaaaattt ttttcatgaa
    25621 agatctcctt ttttattttt at
//
LOCUS       DACDCV010000030        25638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDCV010000030 DACDCV010000000
VERSION     DACDCV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.030"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            361..783
                     /locus_tag="IV041_001241"
     CDS             361..783
                     /locus_tag="IV041_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8382045.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            780..1010
                     /locus_tag="IV041_001242"
     CDS             780..1010
                     /locus_tag="IV041_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382046.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1508..1708
                     /locus_tag="IV041_001243"
     CDS             1508..1708
                     /locus_tag="IV041_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8382047.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1735..2928
                     /locus_tag="IV041_001244"
     CDS             1735..2928
                     /locus_tag="IV041_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382048.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(2996..4561)
                     /gene="guaA"
                     /locus_tag="IV041_001245"
     CDS             complement(2996..4561)
                     /gene="guaA"
                     /locus_tag="IV041_001245"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8382049.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4620..5543)
                     /locus_tag="IV041_001246"
     CDS             complement(4620..5543)
                     /locus_tag="IV041_001246"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8382050.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5739..6344
                     /locus_tag="IV041_001247"
     CDS             5739..6344
                     /locus_tag="IV041_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8382051.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6652..7668
                     /locus_tag="IV041_001248"
     CDS             6652..7668
                     /locus_tag="IV041_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8382052.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7809..9110
                     /locus_tag="IV041_001249"
     CDS             7809..9110
                     /locus_tag="IV041_001249"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8382053.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9156..9818
                     /gene="deoC"
                     /locus_tag="IV041_001250"
     CDS             9156..9818
                     /gene="deoC"
                     /locus_tag="IV041_001250"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8382054.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9839..10237
                     /locus_tag="IV041_001251"
     CDS             9839..10237
                     /locus_tag="IV041_001251"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8382055.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10303..11385
                     /locus_tag="IV041_001252"
     CDS             10303..11385
                     /locus_tag="IV041_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8382056.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11597..13159
                     /locus_tag="IV041_001253"
     CDS             11597..13159
                     /locus_tag="IV041_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382057.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13152..14285
                     /locus_tag="IV041_001254"
     CDS             13152..14285
                     /locus_tag="IV041_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382058.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14285..15241
                     /locus_tag="IV041_001255"
     CDS             14285..15241
                     /locus_tag="IV041_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382059.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15340..16506
                     /gene="deoB"
                     /locus_tag="IV041_001256"
     CDS             15340..16506
                     /gene="deoB"
                     /locus_tag="IV041_001256"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8382060.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16530..17348
                     /locus_tag="IV041_001257"
     CDS             16530..17348
                     /locus_tag="IV041_001257"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8382061.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17365..18072
                     /gene="deoD"
                     /locus_tag="IV041_001258"
     CDS             17365..18072
                     /gene="deoD"
                     /locus_tag="IV041_001258"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8382062.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(18370..19056)
                     /gene="gpmA"
                     /locus_tag="IV041_001259"
     CDS             complement(18370..19056)
                     /gene="gpmA"
                     /locus_tag="IV041_001259"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8382063.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(19228..19809)
                     /locus_tag="IV041_001260"
     CDS             complement(19228..19809)
                     /locus_tag="IV041_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8382064.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(19942..20625)
                     /gene="rpiA"
                     /locus_tag="IV041_001261"
     CDS             complement(19942..20625)
                     /gene="rpiA"
                     /locus_tag="IV041_001261"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8382065.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            20944..21357
                     /gene="rpsL"
                     /locus_tag="IV041_001262"
     CDS             20944..21357
                     /gene="rpsL"
                     /locus_tag="IV041_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8382066.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            21441..21911
                     /gene="rpsG"
                     /locus_tag="IV041_001263"
     CDS             21441..21911
                     /gene="rpsG"
                     /locus_tag="IV041_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8382067.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22005..24092
                     /gene="fusA"
                     /locus_tag="IV041_001264"
     CDS             22005..24092
                     /gene="fusA"
                     /locus_tag="IV041_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8382068.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            24264..25451
                     /gene="tuf"
                     /locus_tag="IV041_001265"
     CDS             24264..25451
                     /gene="tuf"
                     /locus_tag="IV041_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8382069.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8070 a         4749 c         5492 g         7327 t
ORIGIN      
        1 aatttagtca tactcctaga aagtagataa aattggcgac ttaatggagg gaaaagtcgc
       61 catgagaaag ataatctgtc atctagttct cttaattaag ttcttatcaa aaaaacttat
      121 ccacctgtac tggatagata tgaccatcaa tgtggttttg cagattacat agatagaaaa
      181 atcaaagcat atgaacagag aaaacaatct gtttatgtgc ttttttggtc acttaataaa
      241 tatcttttag gatttctctt catttatgca acaaatcccc cgttcgtgtc ggggtttagt
      301 atgaagggag ataaagaagc acatgccact ccctttcacg acagaaaggg ggtgaaaagc
      361 atgaaaccat cttcttttca agaaaccata gaaaatcagt ttgactatat ctgcaaacaa
      421 gttattgaag atgaacgaaa agattacttc aaacatctag acagactatc agcaaaagaa
      481 gtctcgtttt ctgatataga taactacctt gtcaactctt tttctactgt tgatacgtac
      541 gtcactgatt tccaactatt taaattattt ggattaagaa tcaatgttga aagtgattta
      601 ttaagtgaag ccctgcgtca tctttcagag aaaaaacgga acattatttt acttcattac
      661 tttatggata tgagtgatgt ggagattgcg gagttactaa atttgaatcg ttcgactgtt
      721 tatcgtcatc gaataagtgg actagccatg attaaagaat tcatgaagga gtgtaaagaa
      781 tgaaaacagc ttatccaagg gttcccttcc ccctcattgt aaaggcgaca gatggcgacg
      841 tggaagcaat taatcagatt gttaaacatt atcgagggta tacctcaaaa cgttcattac
      901 gtcgcatgac agacgaatat ggaaattctc atatggttat tgatgaaacc ctacgtggca
      961 gaatggaaac gaaactcatc tctaagattt tatcgttcga aattaaatag tcccattttt
     1021 tcgtggaagc gtgtcgaatc gtatccacgc ttcccgattt gggaggtttg ttattccatc
     1081 aaagcatatt gtgtgttcag taggttttga taggctaaca agccaaacgt tctttgacaa
     1141 ccaaataaag gcaatcgaat acgtttcggt aagaaaagag ctgatgaact aacacgccat
     1201 gacctacaaa attagtgagc gattcatgtt agtaattcga aagtaatctt tagcaggatt
     1261 gctgccatga ctttcttatc gtgataatga tactcccgta tagccatagt ccgagcgtta
     1321 gaagcgtcac aggcaatggg tacggctaca tgagaaccat gcagaagtga aattcctgtg
     1381 agctttgcta gagctgttcg attgctaaga tattcttctc ttgtcagtgt attattataa
     1441 aactatttga tataattata ggtgaatatt ggagttttcc tagcctataa ttgggaaagg
     1501 agtaataatg ataacggtca caaaaaagga tttagttgag ctaggatatg gaacttcttt
     1561 ttcagcagac atcataaaaa aagcaaaaga attgatgatt acaaaagggc atatctacta
     1621 tcaatctcga aagctagata gagtccctcg tgaagctgtt gaagaaattc taggaattcg
     1681 gttttcaaat gaaaacaggt ctgagtagtt tttgcacagt aaggagtaaa aactatggtt
     1741 aaagacccaa taaaaaaagc aaagaatgga acctattatt ttcgtgcaaa tctaggttat
     1801 gattctaaag ggaaaaaaat tcaaaaatat cgtagtggat tcactactaa aaaagaagca
     1861 agagaagaat attcgaaact gctattgatg aaaccagaag aattaagtga gaataaggat
     1921 aaaatgacgt ttaaacatta tatctttgaa attttccttc catggtataa gacacaggta
     1981 aagctcagaa catatgaaaa tcgcttacca actattaaga agcatttctc ttattttgat
     2041 aaaatggctg tttccgatat tgagcctatt gatgtgcaaa attggcaact aaaattatca
     2101 aagaagtgta agtcttctta tgtaagagct gttcaaggac tattttcagt tgctatggat
     2161 cgagcaattg tccttgggat aaccaccaca aatccatcta agattattgg caatgtgaaa
     2221 aagcaaaagt ctaaaattga attttggaca aaagaagaat ttgaaaaagt aatctctcta
     2281 atttacaaag aagattacta tcaacacttt ttatttatct ctctgtggtt tttatttatg
     2341 actggaatgc gaattggtga agctactgcc attcaatggg aagatattga ttttgactca
     2401 ggagtactaa ccatcaacaa aacactctat tacaagaatc aatcgaatta tagatttgtt
     2461 gaaccaaaaa caaaggcgag cgttcgtcat attgtattag ataaatgcac attaacttat
     2521 ttatccgaat ggaaggaagt acaacaaaat ctaattcaga cagactttgt aatgagctat
     2581 aacggtattc ctacccaaaa acatacacta tcatatgcta ttgaacgcta tgctaagctg
     2641 gctggtatcc atcgaatcag aatccatggg ttacggcatt ctcatgcttc cttgttaatc
     2701 agtatgggag aaaatccact tatcattaaa gatcgtctag gacacgaaga tattgagacg
     2761 actttaggaa cgtatggaca tctttatcct aacagtaatt tcgaggtagc taacaaatta
     2821 aatggagcca tttcctttaa agaagcaaaa caaaattatg atacttcacc aaagaatcaa
     2881 tttactgtag actacttgcg aaacaaagaa atgaaaaaag tgcaataaaa gtgcagtaaa
     2941 aagataagaa gagccagaaa cccttggtat aagaggattt ctagctctcc tacaactatt
     3001 cccactccac tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg
     3061 ctacttcgtt tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa
     3121 agtcagcggt cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag
     3181 tacgtccgtc acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc
     3241 agatatcacg gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc
     3301 gtacgatttc tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac
     3361 cagggaatgg ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac
     3421 ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg
     3481 gcagtccacc tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga
     3541 taacgtcagt atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt
     3601 ttgttgcttc gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt
     3661 ctgggtcaga tacacctttc aacttgtcta agaagcgatc tcttgcatcg actttgatga
     3721 tgttcaagcc gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta
     3781 gcagaccgtg gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa
     3841 ccccaacgac actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa
     3901 cttgttcacg gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt
     3961 cacagtgaca aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac
     4021 gtacttctgc atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa
     4081 tcggacaatc tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt
     4141 caccatggct catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat
     4201 cgcttgttac ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac
     4261 cgccaagttt gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta
     4321 aattgaagat ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc
     4381 cagaaagaat gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc
     4441 tcaataattc ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact
     4501 gactaccgta gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca
     4561 aatttattac ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc
     4621 taatattttc gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata
     4681 tcggctcttc ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc
     4741 catacatctt ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa
     4801 taattctctg gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat
     4861 ttttcgatca gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg
     4921 tagatttgct gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca
     4981 ggctgctgga tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca
     5041 ggtactttta tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc
     5101 atatcataac tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga
     5161 tttggataaa gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga
     5221 gacaaaatcg tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg
     5281 atcccgataa taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag
     5341 aacaaagatt caaattcttt catataaaga taaatcagat gagcaagagg tacatagata
     5401 tctcgaacat cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct
     5461 tgtgtcaaag gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg
     5521 tagtagttca ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt
     5581 taacacaaaa taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat
     5641 ctttataata gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat
     5701 agactgttgt catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg
     5761 aacgcccaga tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct
     5821 ttcaatttgt aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg
     5881 tcctgattga tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg
     5941 gatgcgggta tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa
     6001 tggtcgacat taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa
     6061 tcgaacatgt tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg
     6121 cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga
     6181 caggcgcata tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac
     6241 aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca
     6301 caaaagataa aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt
     6361 ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg
     6421 ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga
     6481 gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt
     6541 cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct
     6601 tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg
     6661 accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg
     6721 aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat
     6781 gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc
     6841 atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta
     6901 gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa
     6961 gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg
     7021 gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg
     7081 ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag
     7141 gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc
     7201 ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt
     7261 catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg
     7321 actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct
     7381 attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc
     7441 aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac
     7501 atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca
     7561 cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa
     7621 acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata
     7681 ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt
     7741 ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg
     7801 atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga
     7861 aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag
     7921 tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact
     7981 taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa
     8041 aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc
     8101 tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag
     8161 gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag
     8221 aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg
     8281 caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc
     8341 cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt
     8401 tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg
     8461 caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag
     8521 atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg
     8581 aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc
     8641 aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag
     8701 aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg
     8761 gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc
     8821 ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg
     8881 caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat
     8941 tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct
     9001 atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg
     9061 gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag
     9121 cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc
     9181 atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa
     9241 aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac
     9301 aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca
     9361 cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg
     9421 atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata
     9481 tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat
     9541 cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg
     9601 attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt
     9661 tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg
     9721 aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag
     9781 tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat
     9841 ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt
     9901 tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca
     9961 aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt
    10021 tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac
    10081 ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc
    10141 tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc
    10201 actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga
    10261 ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta
    10321 tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac
    10381 ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc
    10441 acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa
    10501 aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct
    10561 tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg
    10621 atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca
    10681 ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca
    10741 actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa
    10801 acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct
    10861 ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg
    10921 aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg
    10981 tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc
    11041 caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac
    11101 cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg
    11161 acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac
    11221 ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta
    11281 acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc
    11341 atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc
    11401 cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat
    11461 ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca
    11521 aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt
    11581 aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa
    11641 agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat
    11701 tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg
    11761 attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag
    11821 tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga
    11881 tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct
    11941 tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt
    12001 caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt
    12061 aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac
    12121 ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc
    12181 gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt
    12241 tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt
    12301 agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc
    12361 tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc
    12421 cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga
    12481 tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg
    12541 acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca
    12601 tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt
    12661 atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct
    12721 gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac
    12781 aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat
    12841 cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact
    12901 ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa
    12961 agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc
    13021 tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga
    13081 attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg
    13141 ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt
    13201 cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca
    13261 tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa
    13321 cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag
    13381 ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct
    13441 gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct
    13501 taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg
    13561 gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat
    13621 ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga
    13681 tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg
    13741 taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga
    13801 aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg
    13861 caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag
    13921 ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt
    13981 cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg
    14041 gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga
    14101 caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg
    14161 tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag
    14221 aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac
    14281 tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg tttattcaac
    14341 accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt
    14401 agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat
    14461 gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg
    14521 tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat
    14581 cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg
    14641 gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg
    14701 attagcagat attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta
    14761 ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt
    14821 gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata
    14881 tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt
    14941 ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat
    15001 ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt
    15061 gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc
    15121 gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt
    15181 tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata
    15241 atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt
    15301 tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg
    15361 atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt
    15421 ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag
    15481 cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa
    15541 ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg
    15601 gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat
    15661 gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat
    15721 agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt
    15781 gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta
    15841 gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg
    15901 attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc
    15961 aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa
    16021 gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc
    16081 actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg
    16141 aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt
    16201 catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca
    16261 gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac
    16321 gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt
    16381 aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg
    16441 attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta
    16501 aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa
    16561 cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt
    16621 caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga
    16681 tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc
    16741 tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca
    16801 tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg
    16861 tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta
    16921 ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg
    16981 gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag
    17041 cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac
    17101 caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag
    17161 ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa
    17221 tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg
    17281 tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat
    17341 tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc
    17401 gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt
    17461 ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat
    17521 aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat
    17581 gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt
    17641 gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt
    17701 tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat
    17761 ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat
    17821 gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa
    17881 cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac
    17941 catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca
    18001 acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg
    18061 atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag
    18121 caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt
    18181 gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat
    18241 ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag
    18301 gagaaaaccg tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta
    18361 tagtgttttt tataggtagt attttttagc aactgttaag tcatcattta attcataaac
    18421 aagtggttga cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat
    18481 atgtttagct agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc
    18541 tagtaatgct ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt
    18601 caagttttct ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc
    18661 tgcagaacct tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca
    18721 gatgtgcact tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa
    18781 tttaccatag tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc
    18841 tgaatattct aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc
    18901 aacatcaaat tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat
    18961 tccttctggt gctagattta catcagccca accagtaaat tggttcaatg cattccattc
    19021 actaagaccg tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg
    19081 tttatatttc tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca
    19141 tcgaaaaatt agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt
    19201 gcccacgcaa ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg
    19261 taaattgata tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg
    19321 gatcgtttct tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat
    19381 ttttggtctt ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg
    19441 ttctttgatc aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt
    19501 tcccatttcg cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt
    19561 gaataattgc gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca
    19621 aatgactagt gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt
    19681 agaatcattt tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga
    19741 taattgatca acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat
    19801 aattttcatg gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg
    19861 tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt
    19921 ggatgatcaa gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt
    19981 ttctgagccg acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc
    20041 aacaagttca tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag
    20101 atcaatgatg taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa
    20161 gacgggttgg aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat
    20221 cacttcaact gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat
    20281 ccaaatcgtt tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc
    20341 gcctttgatc ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac
    20401 tacggggact tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt
    20461 cgcatttgaa gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat
    20521 ctcttctacc atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt
    20581 tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt
    20641 tttttagttt gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata
    20701 ccattctttt ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt
    20761 tttttcattc gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt
    20821 gctgactgtt tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca
    20881 caaaaaaaga accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa
    20941 ggaatgccta caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct
    21001 aattcacctg ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac
    21061 tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac
    21121 tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat
    21181 atcccaggga ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt
    21241 gtaaaagact taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt
    21301 gttaacgacc gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt
    21361 taattattaa acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat
    21421 ttcggaagga ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac
    21481 cagatcctat ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg
    21541 gaaaacgcgg gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta
    21601 caggtaacga tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg
    21661 aagttaaagc acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg
    21721 aacgccgcgt tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac
    21781 acacaatgga agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt
    21841 ctgttaaaaa acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact
    21901 atcgctggta agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc
    21961 ttatttttag caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt
    22021 ctttacaaaa cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg
    22081 ctactgaacg tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag
    22141 gagcttcaca aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg
    22201 ctgctactac agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg
    22261 tagacttcac agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc
    22321 ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt
    22381 atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat
    22441 attcagtatc aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa
    22501 ttggtgcgga agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt
    22561 atacaaacga tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat
    22621 tagctgaaga atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta
    22681 ctttgaaata cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc
    22741 gcgcaacagt taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag
    22801 gggttcaatt actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag
    22861 ctatcaaagg gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg
    22921 ctcctttctc agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt
    22981 tcttccgtgt gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag
    23041 gcaaacgtga acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct
    23101 ctgaagttta cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg
    23161 acacactatg tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag
    23221 ttatccaagt cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt
    23281 tacaaaaact atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg
    23341 aaacaatcat cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc
    23401 gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc
    23461 gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg
    23521 gtcacgtatg gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg
    23581 cgatcgtcgg tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag
    23641 atgctatgga aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt
    23701 acgatggttc ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta
    23761 tggcattacg tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg
    23821 tagaagttgt tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc
    23881 gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg
    23941 ttccacttgc tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg
    24001 gtacatttac aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa
    24061 tcattaagaa aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag
    24121 agattatcca aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata
    24181 tgggcatgtg gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg
    24241 tatttttagg aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg
    24301 ttaacattgg tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca
    24361 caactgtact atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg
    24421 ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag
    24481 atacacgcca ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga
    24541 tcactggtgc tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa
    24601 tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg
    24661 tattcttgaa caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg
    24721 aagttcgtga cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg
    24781 gatcagcttt gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa
    24841 tggctgcagt tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga
    24901 tgccagttga agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg
    24961 aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt
    25021 caaaaacaac agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg
    25081 gagacaacat tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag
    25141 ttttagctaa accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt
    25201 tgacaaaaga agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct
    25261 acttccgtac aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta
    25321 tgcctggcga caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg
    25381 gtactaaatt ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa
    25441 tcaaagctta attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc
    25501 ggaacgaagg tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa
    25561 aagacaggag tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg
    25621 aaactttatg aaaacaaa
//
LOCUS       DACDCV010000031        25480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDCV010000031 DACDCV010000000
VERSION     DACDCV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.031"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IV041_001266"
     CDS             <1..354
                     /locus_tag="IV041_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8382070.1"
                     /translation="KSLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERM
                     EEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQL
                     LEMPIFLMPANEKNN"
     gene            391..1401
                     /locus_tag="IV041_001267"
     CDS             391..1401
                     /locus_tag="IV041_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8382071.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1516..2229)
                     /locus_tag="IV041_001268"
     CDS             complement(1516..2229)
                     /locus_tag="IV041_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382072.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2419..3009)
                     /locus_tag="IV041_001269"
     CDS             complement(2419..3009)
                     /locus_tag="IV041_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8382073.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3493..3720
                     /locus_tag="IV041_001270"
     CDS             3493..3720
                     /locus_tag="IV041_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8382074.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3736..4902
                     /locus_tag="IV041_001271"
     CDS             3736..4902
                     /locus_tag="IV041_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8382075.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5136..6179
                     /gene="hrcA"
                     /locus_tag="IV041_001272"
     CDS             5136..6179
                     /gene="hrcA"
                     /locus_tag="IV041_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8382076.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6273..6836
                     /gene="grpE"
                     /locus_tag="IV041_001273"
     CDS             6273..6836
                     /gene="grpE"
                     /locus_tag="IV041_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8382077.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            6887..8716
                     /gene="dnaK"
                     /locus_tag="IV041_001274"
     CDS             6887..8716
                     /gene="dnaK"
                     /locus_tag="IV041_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8382078.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8867..10033
                     /gene="dnaJ"
                     /locus_tag="IV041_001275"
     CDS             8867..10033
                     /gene="dnaJ"
                     /locus_tag="IV041_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8382079.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10115..10780)
                     /locus_tag="IV041_001276"
     CDS             complement(10115..10780)
                     /locus_tag="IV041_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8382080.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10756..10929
                     /locus_tag="IV041_001277"
     CDS             10756..10929
                     /locus_tag="IV041_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8382081.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(10974..11711)
                     /locus_tag="IV041_001278"
     CDS             complement(10974..11711)
                     /locus_tag="IV041_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8382082.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11713..13215)
                     /locus_tag="IV041_001279"
     CDS             complement(11713..13215)
                     /locus_tag="IV041_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8382083.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13474..15468
                     /gene="uvrB"
                     /locus_tag="IV041_001280"
     CDS             13474..15468
                     /gene="uvrB"
                     /locus_tag="IV041_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8382084.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15478..18294
                     /gene="uvrA"
                     /locus_tag="IV041_001281"
     CDS             15478..18294
                     /gene="uvrA"
                     /locus_tag="IV041_001281"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8382085.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18557..19822
                     /locus_tag="IV041_001282"
     CDS             18557..19822
                     /locus_tag="IV041_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8382086.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19826..20557
                     /locus_tag="IV041_001283"
     CDS             19826..20557
                     /locus_tag="IV041_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8382087.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20692..21576
                     /gene="rapZ"
                     /locus_tag="IV041_001284"
     CDS             20692..21576
                     /gene="rapZ"
                     /locus_tag="IV041_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8382088.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21573..22571
                     /locus_tag="IV041_001285"
     CDS             21573..22571
                     /locus_tag="IV041_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8382089.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22605..23540
                     /gene="whiA"
                     /locus_tag="IV041_001286"
     CDS             22605..23540
                     /gene="whiA"
                     /locus_tag="IV041_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8382090.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24083..24733
                     /locus_tag="IV041_001287"
     CDS             24083..24733
                     /locus_tag="IV041_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382091.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            24920..>25480
                     /locus_tag="IV041_001288"
     CDS             24920..>25480
                     /locus_tag="IV041_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8382092.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8397 a         4321 c         5453 g         7309 t
ORIGIN      
        1 aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg
       61 aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca
      121 tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat
      181 gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt
      241 gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc
      301 cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt
      361 aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat
      421 gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc
      481 gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt
      541 tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa
      601 atgatgcaag atgaaaatat ccaaatgatc tttccgactt ggggaggaga attattgatt
      661 gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca
      721 gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga
      781 actaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt
      841 gtactgaaaa caagagaagg agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa
      901 gtgtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg
      961 caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg ttgtattgat
     1021 acgatcaggc atttaatcgg tactccttat ggagatgtcc aatcttttaa aagaaagtat
     1081 aatgatggaa agccgatctt gtggtttcta gaaaactgtg agctatcgtc agttgattta
     1141 agaagatcac ttgttcagat gaaactggca ggctggtttg atggcagctc ggggatcatg
     1201 ttcggtagaa gtgcggcaga ggatttgtta gattataaaa tgaaggatgt atacgaagat
     1261 ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca
     1321 caactgacat tgattaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact
     1381 gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtctatca aagaccaata
     1441 aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat
     1501 aacatattag aaagtctaat tatctataac tgtcacatcg attaactgtt tcacgtcatt
     1561 gggatttcct gtaggaatag ctactacaga tgcattatca atgatttctt gagttgtttc
     1621 tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg
     1681 ataactagtg attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta
     1741 tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat cataaggggc
     1801 gataggtgtt cctgcaggat aatgaataac atgtaattcg aaatcacccg atgtatctct
     1861 aaaagtatta cctctaatag ctagccaaac acgtgcgtac tcaatttgta gatcagaata
     1921 tcctgctaac ggatctattg actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga
     1981 agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt
     2041 actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc
     2101 agctgaactg gtttgtctga ttgaactttt gtctgtggtc gcagatggat cactattttc
     2161 tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa
     2221 ctttttcata taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt
     2281 ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg tatcttcttt gataaatgtc
     2341 agaaaaagtg tgacaaataa tcatcagtaa aattacttgt cacactattg ataaggagta
     2401 tttaattgaa gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa
     2461 tccaagtttg tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc
     2521 ttgaagaatc aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg
     2581 atctttcaca gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa
     2641 ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa
     2701 ataatcaact gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat
     2761 tatttgatat aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag
     2821 gtcaactttt tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat
     2881 ggaagtgacc tctgattgga tagaagcagt tttgttttgc catttctcat tttggtttag
     2941 tgtgctagta atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc
     3001 agttttcatt ttcaagctct cctttactat ctataacaag gacaacagtt ttaacctaca
     3061 gacattcatt aaaattgggt agcgacagcg aaaacaacaa ttcttaagtt ttagacagag
     3121 tatacagata gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt
     3181 gtttaccagc attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc
     3241 ttgagtacaa tattaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa
     3301 tcatatcggg cactcagaca tgaaaacaac cctagagata tataactagg taaccagttt
     3361 aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc
     3421 aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt
     3481 aaggaggaat ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag
     3541 gtcagctttg cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac
     3601 aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat
     3661 gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa
     3721 ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac
     3781 attcatatcc cattttgcga gcatatctgc tattactgtg attttaataa agttttttta
     3841 gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgaa aataatgcta
     3901 gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag ggggcgggac accaacttct
     3961 ttatctgcta aacaacttga tcgactgtta tcaggtgcta gagagatcct gccttttcaa
     4021 aatggtaatg agtttactgt agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa
     4081 gtaatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt tgataatcgc
     4141 ttattgaaaa aaattggacg taaacacacc gcagaagatg tgtatcaaac tatgcaattt
     4201 ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa
     4261 acactagaag gatatctgga tactttggaa aaagctttgg ctttggatct gcctcactat
     4321 tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga
     4381 agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca
     4441 atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa
     4501 tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct
     4561 agcggttatc ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc
     4621 ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa
     4681 atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc
     4741 tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag
     4801 caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggaccttt
     4861 ttggggaaca atgtgtttga gcgctttttg tttgaaaaat agtttaaaga atatttgttt
     4921 tttatctgaa tttaaataaa aaaagttcaa aaatacaata aaggactatt gaaacgttga
     4981 tataactgga tttacatcaa ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa
     5041 atggagtctt tctcttgaca ggagaagggc ttcttgctat atttataagt ggattagcac
     5101 ttagaatata tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga caaaacgaca
     5161 ttttacgtct gatcatccaa cactatacga gtagtggcgt gcccgttggt tccaaaacat
     5221 tgatgtctga gggagtcaag gccagctcag caacgattcg caatgatatg aaagcattag
     5281 aagatgaagg attgctgttg aagactcact cttcttctgg gcggattcca tctgctttag
     5341 gctatcgtta ttacgtggat catttgttaa aacctgctcg cgtagcagaa gatgatttgc
     5401 tgctgattcg tcagtctcta ggcagagaat ttcatgaaat caatgagatt attaagcaat
     5461 ctgctgagat attatctgag ctgacaagct atacgacttt ttcattggga cctgaaataa
     5521 aagaccgccg actgactggc tttcgtatcg tacccttgaa taatcgacag attattgcta
     5581 tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt cactcttccc gaatatatgg
     5641 gaagtcagga actagaaaaa atggtacaga ttgtaaacga ccgtctagtc ggagatcctc
     5701 tgatgacggt gtaccataaa cttcgtacgg agattccgat gattcttcat aaatatttcc
     5761 aaacgacaga agggatgtct cagttatttg acaccgttct tggtcatgtt tttgaagata
     5821 aggtttatgt cagtggacaa atgaatctct tggattatga gccccgtcaa gatcttgatc
     5881 agttcaaatc gatgttttct tttatgaaag actcagacgg acttacacaa atgatcactc
     5941 cgatggatag caatattcat atcaagattg gtgctgaatt aggcaatgat ttattgcaga
     6001 atatgagtat gattcaagca agttacgaga tagatggtca tggaagaggt acaatagcct
     6061 tgttagggcc tactagtatg ccttattctc ggatgttcgg tctagtcgat gtttatcgaa
     6121 gagaactagc agctaaactt gctgattact accgttcgct tgacttgtca aactcgtaat
     6181 gtgaataacg aagctatttg cagctgatcg ttctctcttt cttagtaaga gcaatcaaac
     6241 agctgactga ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa gaactagata
     6301 aagaaatgac agatgctcag ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg
     6361 gtatttcaga agctgaagcg gaagaattcg aaacagcgaa attaaaagct gagttagaag
     6421 aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa
     6481 aaaatgagcg tgagcaatta caaaaatacc gttcacaaga tttagccaaa aaattacttc
     6541 catcaattga caacctggaa cgtgcattag ccacagaagt gtcagatgat caaggggcag
     6601 gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg taacgcactg gaagaagaag
     6661 gaattgaaaa aatccctgct aaaggagaag cctttgatcc taatcttcat caagcagtac
     6721 aaactgttcc agcgactgaa gatacaccag ccgatacgat tgtagaagta ctacaagaag
     6781 gctacaaatt acatgatcgc gtgttaagac caaccatggt cattgttgca caataattgt
     6841 gcggatataa aacttacact ttcaattaaa gaaggagacg ttaattatga gtaaaattat
     6901 cggtattgac ttaggtacga ctaactctgc agtagcagta ttggaaggaa acgaagcaaa
     6961 aatcatcgct aacccagaag gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg
     7021 agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc
     7081 atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta gatgtagaag gtaaatcata
     7141 tacaccacaa gaaatctcag caatgatcct tcaatatatc aaaggattcg cagaagacta
     7201 tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca gcttacttta atgatgcaca
     7261 acgtcaagca acaaaagatg ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa
     7321 tgaaccaact gcagctgctc ttgcctatgg tttagataaa acagatcgcg acgaaaaaat
     7381 cttagtattc gaccttggtg gcggtacttt tgacgtatct atccttgaac taggagacgg
     7441 tgtctttgat gtattgtcaa cagctggcga caaccatcta ggtggggacg actttgataa
     7501 caaaatcatc gactacatgg tagcagaatt caaaaaagaa aacagcattg acttgtctca
     7561 agacaaaatg gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc
     7621 tggcgtatca agcacacaaa tcagcttgcc atttatcaca gcaggagaat ctggaccatt
     7681 gcatttggaa atgacattga cacgtgcaaa atttgatgaa ttaacagctg acttagtaga
     7741 acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca ggtttatcac aatcagaaat
     7801 cgacgaagtt attttagtcg gtggatctac tcgtatccct gcagttgtag aagcagtacg
     7861 taaagaaaca ggaaaagaac caaacaaatc agttaaccct gatgaagtag ttgctatggg
     7921 tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt
     7981 tacaccattg tcattaggta tcgaaacaat gggtggagtg ttcactaaat tgattgaccg
     8041 caatacaacg attccaacaa gtaaatcaca agtattctca actgcggcag ataatcaacc
     8101 agcagtagac atccatgtat tacaaggtga acgcccaatg gctgctgaca acaaaactct
     8161 aggaagattc caattgacag atattccagc agctcctcgt ggtattccac aaatcgaagt
     8221 aacattcgat attgataaaa acgggattgt aaatgtatct gcgaaagatc taggtacaca
     8281 aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg
     8341 tatggtaaaa gatgcagaag caaatgcaga agcagataaa gctcgtaaag aggaagttga
     8401 cctacgtaat gatgtagatg cattgctatt ctctgtggac aaaacattga aagaattaga
     8461 aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc
     8521 cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat
     8581 cgttcaaaac ttgactgtta aactatacga acaagcggca caacaacaag ctcaagaaaa
     8641 cccagaagca gctcaaggcg gagcagatga tgtcgttgat gctgattttg aagaagtcga
     8701 tggcgacgat aaataatcag ttcactgaat ttcgagagaa ggccaaagtt gctactttgg
     8761 ctttttcttt tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa atatgatatg
     8821 ataacaagcg acgtgaaaat gatgagaacc aataggagga aagcctatgg caacaaaacg
     8881 tgattattat gaagtcttag ggctctcaaa aggagcatca gaagatgaaa taaagaaagc
     8941 ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat aaagaacctg atgcggaaga
     9001 aaaattcaaa gaagtatcag aagcatatga gattttaagt gacccgcaaa aacgcgctgc
     9061 atatgaccaa tacgggcatg caggcacaga tccaaattac ggtgctggtg gagctggtgg
     9121 cggatttggt ggattcggcg gcttctctgg tggcggcttc ggtggttttg aagatatctt
     9181 cgactctttc tttggaggag gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc
     9241 tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg atttttggtg tagaaaaaga
     9301 aatcaagtat aatcgggaag aaatctgtca tacttgtggt gggaacggtg caaaaccagg
     9361 cacacaacca actacttgtc ataaatgtca tggtgcaggt acgattaatg tagaacgcca
     9421 aacaccactt ggacgagtga tgagccgtca aacttgtgat gtttgtcatg ggacagggaa
     9481 agaaatcaaa gaaccttgtc caacctgtca tggctcagga catgagaaga aagcacattc
     9541 agttaaagtg aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt tagctggtca
     9601 aggagaagct ggagaaaacg gcggaccatt tggtgatttg tatgtggtct tccgtgtaga
     9661 agaaagtgat atctttgatc gtgaaggttc tgaaatttac tatgaattgc ctttaagttt
     9721 tgtacaagca gctttggggg atgaagtcca agtaccaact gtccatggag atgtgaaatt
     9781 gaagatccct gctggaacac agacaggtac gaacttccgt ttacgtggaa aaggcgctcc
     9841 gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga tcacaccgaa
     9901 aaatctaaat gaacaacaaa gagaagcatt acgtgcattc gctgaagctg gcggcatcaa
     9961 agttgaagaa cagcaagaag acggtttctt cgataagttt aaagatgctt ttggcgggaa
    10021 aaagcggaag tagtttttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
    10081 acaatcatat gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
    10141 gctttagaat atgataatcg tcatagtttc taggggtata accagtgaga gaatggaact
    10201 tgttcaatcg atattgcaag gtgtttttat gaataaataa ttcttctgag caatttttga
    10261 tgcttccgtt atttttactg aagcatgcca aagtcttctt gaacaaatgt acttcttctt
    10321 ctgtcaggct tttcaaaata cgttttacat ataatgttcg ttgatctgac gggattgctg
    10381 gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca gattgatgga
    10441 aagctatcca ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg
    10501 tataacaaag cgacccaatt ccaaagttta gctgtaactg atacatttta ttaaactttg
    10561 tctggatttt atcaagcaat gatagcaatg aggctctgga aatccgattg actaggataa
    10621 tgatttgttg tctatgtaca gtgagcaaag cctgtccgta ataagagaag accctttcga
    10681 gcaactccaa taaaggattg atccgttctt gacacaattg ctgattgtca gaaagttgtc
    10741 ccacaactac ataatatgga gaagaaaaat caagctcaat taagtgaggg tggtatcgac
    10801 tatgtacttg atatcatcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag
    10861 cgattgcttg tttgtgcagg aaaacaagcg atgcggctga tacgattagg tgagaaaata
    10921 agaagataaa aatacacggt cccttcatgg cgaccgtgca tttttggttt ttattaaata
    10981 tttaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt
    11041 tgaggcgttc catcttctaa aaagtttccg tcgtcaatga acatgacgcg attggccact
    11101 tctttagcga accccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac
    11161 tttttcatga cgttcaagac atcaccgacc atttcaggat cgagtgcaga agtaggctca
    11221 tcaaaaagca tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgtttt
    11281 tgtccaccag aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc
    11341 agtaatttga ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt
    11401 gctaaaatga tgttttccaa aatagaaaga tgagggaata ggttaaagtg ttggaatacc
    11461 attccgatat gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg
    11521 tcaataataa tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caagaatgta
    11581 ctttttcctg aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggataca
    11641 ttgatgtcgt ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt
    11701 ttttctgcca tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt
    11761 gatcaaaatc aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg
    11821 tgcaacaatg atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt
    11881 tgtgtctttt aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc
    11941 ttgcggcaaa atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc
    12001 ttccatttgt ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc
    12061 acttgcgtta agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac
    12121 gataccaggc aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat
    12181 gatatcgaca taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa
    12241 tccaaaaatc acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt
    12301 tctccaaagt cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga
    12361 ttcatcagca gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat
    12421 aatcttatcg tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag
    12481 aagctcagga ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt
    12541 gatcggtgtt tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc
    12601 catcattgca tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa
    12661 atatttgata gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt
    12721 accaactttt gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc
    12781 tccttttttg acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct
    12841 tttctttcgt tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc
    12901 caaagcttgc aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag
    12961 tttggcgatg ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg
    13021 aaattcaaat ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc
    13081 tattttttgc attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc
    13141 aacatgacca aacgcaaatg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa
    13201 atgctttctt ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa
    13261 gtgctgaatc agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat
    13321 tttatgttat aaaacataac atgtataata agataaaaaa gtggcgaaag tatgttcggt
    13381 gtggtataat tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa
    13441 atgaaaagga atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac
    13501 tttgagcttg tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta
    13561 gtggatggcg tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg
    13621 aaaacatata cgatttccaa tcttattgag aaagtgaata aaccaacact gattattgcc
    13681 cataacaaaa cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat
    13741 gctgttgaat attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca
    13801 agcgatacgt atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat
    13861 tcggctacta gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt
    13921 atttttggat taggctcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga
    13981 gcagaactag atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat
    14041 gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg
    14101 gcttcaagag atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc
    14161 cgagaagtca atgcactgac aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc
    14221 ccggcgactc actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa
    14281 gcggaattag aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa
    14341 cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg
    14401 ggaattgaaa attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg
    14461 ctattggatt tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg
    14521 cctcaaatca gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac
    14581 ggtttccgtt tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa
    14641 catgtcaatc agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca
    14701 gataccgtta tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt
    14761 cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa
    14821 gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat
    14881 ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga
    14941 acagaaatca ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg
    15001 cttcgtgaag gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa
    15061 gaaggctttt tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat
    15121 gaagaaggta aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg
    15181 gatgaaacgt caagacgtcg tacgattcag cagaagtaca atgaagaaca tgggatcgtt
    15241 ccaaaaacga tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat
    15301 gatacaaaag aagcggtaca agtctcgtac gaagaaatga cgaaggaaga aaaagacacg
    15361 ttattgatga aattagaaaa agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca
    15421 gccgcgaatg tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg
    15481 gctaacgata aaattacgat tcatggtgca cgtgcccata acttgaaaaa tatagatgta
    15541 acgattccac gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc
    15601 ctagcatttg atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct
    15661 tatgctcgtc agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg
    15721 agtccggcga tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt
    15781 actgtgacgg aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt
    15841 tgtcccaacg atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta
    15901 atggctttgc ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa
    15961 ggacaacata aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc
    16021 gatggtgaga tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat
    16081 atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg cctatttgat
    16141 tctttagagg cggccctgcg attagcagac ggctatgctc tggttgacgt gatcggtgaa
    16201 gaagaaatgt tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa
    16261 ctagaaccaa gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt
    16321 ttaggtgtca aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt
    16381 agtcaaggtg ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta
    16441 gcgcaggctt gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa
    16501 gagcatcagg agatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat
    16561 gattttggtg gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag
    16621 cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca
    16681 gaattaactt gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa
    16741 atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc
    16801 ttctcaaact taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa
    16861 gtaaatgaca gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc
    16921 gcttccggaa cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga
    16981 tcgaatcttt ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc
    17041 gacaacgatc gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt
    17101 gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt atttgattga cgtcggacct
    17161 ggggccggag aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat
    17221 gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa
    17281 gaacgccgta aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg
    17341 aaaaatatta gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt
    17401 tctggaaaga gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat
    17461 aagaattctg ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag
    17521 atcatcgata tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat
    17581 acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg
    17641 ggttataaga aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc
    17701 ggggatggaa ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa
    17761 gtatgtcatg gtaaacgata taattctgaa acattagaag tgcattacaa aggaaaaagc
    17821 attgctgata ttttggaaat gacagtagag gatgctgtag aattcttcaa gcacattcca
    17881 aagattcatc gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg
    17941 caaccagcaa cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg
    18001 cacaaaatct ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat
    18061 agcgatgaca tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca
    18121 gttttagtga ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta
    18181 ggaccagaag gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc
    18241 attaacgtaa aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga
    18301 tagatagctg agatagttaa aaaaacataa aaaaatgaat ttttgcttgt tttattaata
    18361 tcagtgttct agaatcatta catggtagtt ctttctacac tgattggtat agataaaaac
    18421 ttcctataag ttcaatccgt tcaaaaaaga attgagcaat cggaggactt tcctttgatt
    18481 gcttttttct ttatggtatg atattccttg tgtagttgga aaagaaaaac aagagattat
    18541 gagtaagtga gagataatga tgaacagtat tgaaaatgaa gaatttatcc ctatattatt
    18601 aggaagcgac atgaacgttt atggtatggc acgttctttt aatgaagcat acggcaaaat
    18661 ctgccaagca tatgcttcgg atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt
    18721 agaagttatt ccaggatttg ataaagaccc tgtatttatc gagacgatgc ttcgtttggc
    18781 aaaagaaaga tattctgata agagtaaaaa atatcttttg attgcttgtg gtgatggata
    18841 tgcagaattg atctcccaac ataaacaaga gctgtcagaa tattttatct gtccatatat
    18901 tgattattct ttatttgaaa ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga
    18961 atatgatctt ccgtatccta aaacattgat cgtcagagaa gaaatgttag taaatggaca
    19021 tttggaacag gaattgcctt ttgaattccc agttgctttg aaacctgcaa acagtgtgga
    19081 gtatctttct gtccaatttg aaggaagaaa aaaagctttt attttagaga ctagagaaga
    19141 gttcgattta attttaggcc ggatatacga agccggttat aaaagtgaaa tgattgtaca
    19201 agattttatc ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga
    19261 tcatcaagtt cggatgatgt gccttggtca tccactatta gaagatccta caccagcatc
    19321 gattggtaat tacgttgtga tcatgccaga ttataacgaa aaaatctatc aaaccattaa
    19381 ggcatttttg gaaaaaatcg aatatacagg ctttgccaat ttcgatatga agtatgatcc
    19441 tagagatgga gaatataaat tatttgaaat caatttgaga caaggtcgta gcagtttctt
    19501 tgtgacattg aacggcttga atctggctcg ttttgtcaca gaagatcgag tgttcaacaa
    19561 accctttgtt gaaacgacct atggaacgaa tcaatcagat aaagcaagat tatggatggg
    19621 tgtgcccaaa aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa aactagctga
    19681 gcaaatgatc aaagaaaatc gttacggtac tactgtattt tatgaaaaag accgatcaat
    19741 caagcgctgg ttgttgatga aatatatgtt ccataactat attccaagat tcaaaaagta
    19801 ttttcatgtg aaagaaggct agttcatgga gaattttttc agtattttag gcggaatggg
    19861 cacgatggcg acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga
    19921 tcaagaatat ttgaattatg tcttattcaa tcatgcaaca gttcctgatc gtacagctta
    19981 tattttagat cgatcagaag aaaatccaat gccgtttctg ctggatgata ttgagaaaca
    20041 aaatttattg cgaccaaact tcattgtgtt gacttgtaac accgcacact acttttttga
    20101 agagttacaa gcggcaacgg atattcctat cttgcatatg ccaagagaag cagcaaatga
    20161 acttgtccgt cagcatacga cagggagagt agcgattcta ggaacagaag gaagtatgaa
    20221 agctggaatc tatgaacgcg aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac
    20281 tgcgcttcaa gaaaaaatca attacctgat ctatcatgag atcaaggaat cggattattt
    20341 gaatcaagag ctctattacg aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa
    20401 agtcatccta ggttgcacag aattatcttt gatgcatgaa tttgctgaag ataatcatta
    20461 cccagtgatc gatgctcaat ctattttagc ggatcgaacg atcgaacgtg cattagctga
    20521 aagaagcgag gcattggata cagcatcgga aaagtaaaaa aattggattt ggggcaaata
    20581 tcctaaaaga tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac
    20641 gcccgggatg atataatttt attatgcaca atgtgagagg ggttcgtaaa tatgacagat
    20701 aatttgcaat tagtcatcat aacaggtatg agtggtgcag ggaaaacagt agctatccaa
    20761 agttttgaag atatgggcta tttttgtatc gataatatgc cgcctcgatt gattccaaaa
    20821 ttctgggaat tgatcaaaga atcaggtaaa gtcacaaaaa tggctttagg agtggatttg
    20881 cgttctcgtt ccttctttga agaaatccaa aacatgctga tcgaaatcga aaatacgaac
    20941 ttcatcgaca cttctatctt atttttagat gcttcagacg aagaactagt ggctcgctac
    21001 aaagaaacaa gacgagcaca tccaatggcg atggacggtt tgatcactga agggatacgt
    21061 aaagaacggg cgatactaga cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca
    21121 acattgacac cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc aaagggagac
    21181 caaggatttc ggatcgaatt tgtatcattt ggttttaaat acgggttgcc gatcgattcg
    21241 gatattgtga tggatgtgcg ttttttgccc aaccctcatt atatcccgga actaagacca
    21301 ttgaccggaa aagatcagcc agtctatgat tatgtgatgt cctttgatga aacgcaatca
    21361 ttctaccata aatttttgga tcttctggaa actgtattgc ctggatacat taaagaagga
    21421 aaaagcagct tgacagttgc aatcggctgt acaggcggtc aacaccgttc tgtggcattg
    21481 actgaacgtt tggcaaatgc tttagggaaa aactataaag tcaatgtgac acatcgggac
    21541 aaggataaaa ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa
    21601 aaccaaagat cgtagtggtc ggcggtggca ctggtctgcc agttatcctg aaaagtttac
    21661 gaaatcaagg agcagatatc acagcagtag tgactgttgc agatgatggt ggaagcagcg
    21721 gtgcaatcag agaatcgatc gctatggctc ctcctggtga cttgagaaat gtcttagtgg
    21781 cgttatcgga tatgccacaa ttctatgaag atattttcca ataccgtttc aaaaaggaag
    21841 atcagttttt agccaatcat acgatcggta atctgatcat tgccgcagtt tccgagatgc
    21901 ggggaagcac ttatgaggcg attcaattat tagcaaaaat gatgcatgta gatggacatg
    21961 tttatccttc ttcagaaact cctttgacct tgcatgctgt ttttaaagac ggaacgactg
    22021 cagtaggaga atctaaaatc gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca
    22081 acacaaatga taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca attatggacg
    22141 ccgacatggt cgttttggga cccggaagtt tattcaccag tatccttcca aatttagtga
    22201 taccagaaat cggcgaagca atgctcaaga caactgctga aaccgtctat atctgtaata
    22261 tcatgaccca aaaaggggaa acggaacatt tcacagatgc cgatcatata cgtgttttac
    22321 acaagcattt gaatcagcct tttatcgata cagtattggt caatacggaa aaagtgcctg
    22381 aagattatat ggacccagag atctatgatg agtatctagt acaagtcgca cacgacttca
    22441 acggattgag aaatgaagga tgtcgagtga tttccaccga ctttttagaa ttgaaagatg
    22501 gtggtgtctt ccacgatgga gacaaagtag tagaagaatt gttccgttta gttttcggtt
    22561 caaaatatta agcaatttat tgttggagaa aggaggagaa taggatgtca tttgctgcag
    22621 atgtgaagaa agaactaact ggtttggaag ttcatcgaga acatgcgaaa gcggaattag
    22681 ctgctttgct gcgaatgaac ggctctttag gtattgtgaa ccagcagttt attttgaacg
    22741 tccaaacaga aaatgctgca attgccagac gtatctattc tcttttgaaa gatcattatc
    22801 aggtacgaag tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata
    22861 tcgtccggtt gaagcaaggc acaaaagatg tgttgatgga tcttgatatc atggatggta
    22921 tgatgttcaa tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt
    22981 acttacgtgg tgcttttatg gcctcaggtt cagtcaataa tccggaaacg agtcgttatc
    23041 atttggaaat attttctatt tatgaacagc ataaccaaga catttgcgat atgttaaatt
    23101 attacggtct acatgctcga acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag
    23161 gagcagaaaa aattgctgat ttcttaacat tgattggtgc aaccaactca atgctccgat
    23221 ttgaagatgt tcgaatcgta cgggatatga gaaattcggt caatcgtcta gtcaattgtg
    23281 agacagcaaa tctgaacaag acgattgatg cggcttctaa acaaatcgaa aacatcgaat
    23341 tgatcgaagc aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac
    23401 ttcggcttca acatccagag gtcagcttga aagaattagg ggaaatgatt ccctcaggtg
    23461 caatatcaaa atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac
    23521 tgcgtgaaga agcagtttaa tccaaagaaa aaactgatac atgaagcagg agatgcgaat
    23581 gtatcagttt tttttaaaag ctatacttgc ttttcttccg tcaaactata attccaactt
    23641 tactggagta acataatgtt gaaaggcttc tattgttttt taatacaatc gtataaaaag
    23701 cggggagaca actgaatgaa gattttagtt ttatacataa acttttgctt aatatagaag
    23761 tcctgatata gataggatca agtatttatc tataactgga gattaattaa aaactggaag
    23821 gaactgctcg atcaaccttg agaactgttt ttcttacttc tggtcaagat tctcgtggtt
    23881 ggtggataag gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa
    23941 ttaatgtaga agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca
    24001 taaatagtaa ttgggatgta tggttggtat acgttatacc tgaacttgaa ctatcaagat
    24061 ttggtttaga atttgagaat cgatgtttat aaagggaggg caaaaaatgt gcttttttac
    24121 aacttttact atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa
    24181 tacagcattt ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat
    24241 cgttggattt tttctattca cagttattgt atttatttgg agaactacag caccaccatt
    24301 gccagagatt acatatggag aatttccgtt tagtcttgaa tatgaaatag atgaggaaag
    24361 aattttgatt gaagatacgg tgattgcaga atttatagga agttttagag ggaatgtgac
    24421 aactcaacct agacgaagtt ggagtataaa acttttaaat aatagtggag agttgaaagc
    24481 atttcattat gaattcttaa taatagaaag tgaagatgta acaatcactt taagtagtgg
    24541 ctctgctgga tattttatga atgatggtgg aagcaagcca cagctcacag tccgtgatcg
    24601 tattgcaggt tttatgcatt tttctcacta tgacttagaa gaagctcgta aaatacttgc
    24661 agaatatggg atcacattaa tcaattggga atatgcgcca ccgataagaa attttttcaa
    24721 tgattattat tagtcttata aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt
    24781 ttccattttt tgtttgatag atatatagca ttgttgaagc atgggatagt ggtgccaatt
    24841 ttatcaatga ggttggtcag gcagttaaca taattggtga tgctataaat ccatttaatt
    24901 ggaattggag gaattaggtg tggaaacagt gagattatgg atattcatga aaaattttcg
    24961 ttcggctttg agatatgtta ttctggaatc aaaatcaggt tattatctga ttgacacaga
    25021 tcactatact ttttggggag gctacctttt tccgattttt aattggataa tcccccataa
    25081 ggttatgaaa attaacttgc ctgcagaaga aataaataga ttaatactca acaaagattt
    25141 acaaaggaaa agagagaaag aacattctaa tgttgcttgg ataggtccag catttgcagt
    25201 agtgacttct tcaattgtcg gtcctatgct agagagtatt tatagtgatt tttctattgg
    25261 aatacgttat ttatttgtta ctttaagtat agctgttctt ttgtgcataa agggttatgt
    25321 tagcaagaag aataaagata ttttaacact aatcggtgta aacaatttat ctagtactaa
    25381 aattatactg tttcctgaat ctataaaaga aggatcaagg ttgttgataa tttttataat
    25441 gcttatactg ttttctgttt tatttggggt gatgtttata
//
LOCUS       DACDCV010000032        25437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDCV010000032 DACDCV010000000
VERSION     DACDCV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.032"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..3596
                     /locus_tag="IV041_001289"
     CDS             69..3596
                     /locus_tag="IV041_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8382093.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3593..7315
                     /gene="addA"
                     /locus_tag="IV041_001290"
     CDS             3593..7315
                     /gene="addA"
                     /locus_tag="IV041_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8382094.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7384..7725
                     /locus_tag="IV041_001291"
     CDS             7384..7725
                     /locus_tag="IV041_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382095.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8034..9077
                     /gene="pheS"
                     /locus_tag="IV041_001292"
     CDS             8034..9077
                     /gene="pheS"
                     /locus_tag="IV041_001292"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8382096.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9082..11502
                     /locus_tag="IV041_001293"
     CDS             9082..11502
                     /locus_tag="IV041_001293"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8382097.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11672..12682)
                     /gene="trpS"
                     /locus_tag="IV041_001294"
     CDS             complement(11672..12682)
                     /gene="trpS"
                     /locus_tag="IV041_001294"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8382098.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12936..13673
                     /locus_tag="IV041_001295"
     CDS             12936..13673
                     /locus_tag="IV041_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8382099.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13687..14502
                     /locus_tag="IV041_001296"
     CDS             13687..14502
                     /locus_tag="IV041_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8382100.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14515..15162
                     /locus_tag="IV041_001297"
     CDS             14515..15162
                     /locus_tag="IV041_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8382101.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15178..15834
                     /locus_tag="IV041_001298"
     CDS             15178..15834
                     /locus_tag="IV041_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8382102.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15996..16817
                     /gene="racE"
                     /locus_tag="IV041_001299"
     CDS             15996..16817
                     /gene="racE"
                     /locus_tag="IV041_001299"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8382103.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16820..18175
                     /gene="rph"
                     /locus_tag="IV041_001300"
     CDS             16820..18175
                     /gene="rph"
                     /locus_tag="IV041_001300"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8382104.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18176..18694
                     /locus_tag="IV041_001301"
     CDS             18176..18694
                     /locus_tag="IV041_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8382105.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18801..19301
                     /locus_tag="IV041_001302"
     CDS             18801..19301
                     /locus_tag="IV041_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8382106.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19581..20501)
                     /locus_tag="IV041_001303"
     CDS             complement(19581..20501)
                     /locus_tag="IV041_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8382107.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20666..21679
                     /locus_tag="IV041_001304"
     CDS             20666..21679
                     /locus_tag="IV041_001304"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8382108.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21789..22544)
                     /gene="fabI"
                     /locus_tag="IV041_001305"
     CDS             complement(21789..22544)
                     /gene="fabI"
                     /locus_tag="IV041_001305"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8382109.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22764..23204
                     /gene="fabZ"
                     /locus_tag="IV041_001306"
     CDS             22764..23204
                     /gene="fabZ"
                     /locus_tag="IV041_001306"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8382110.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23319..24080)
                     /locus_tag="IV041_001307"
     CDS             complement(23319..24080)
                     /locus_tag="IV041_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8382111.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24269..25363
                     /locus_tag="IV041_001308"
     CDS             24269..25363
                     /locus_tag="IV041_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8382112.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8418 a         4419 c         5374 g         7226 t
ORIGIN      
        1 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
       61 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      121 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      181 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      241 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      301 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      361 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      421 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      481 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      541 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      601 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      661 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      721 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      781 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      841 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      901 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
      961 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1021 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1081 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1141 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1201 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1261 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1321 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1381 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1441 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1501 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1561 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1621 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1681 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1741 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1801 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1861 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1921 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     1981 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2041 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2101 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2161 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2221 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2281 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2341 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2401 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2461 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2521 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2581 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2641 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2701 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2761 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2821 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2881 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     2941 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3001 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3061 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3121 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3181 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3241 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3301 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3361 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3421 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3481 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3541 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3601 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3661 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3721 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3781 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3841 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3901 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     3961 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4021 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4081 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4141 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4201 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4261 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4321 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4381 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4441 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4501 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4561 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4621 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4681 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4741 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4801 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4861 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4921 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     4981 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5041 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5101 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5161 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5221 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5281 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5341 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5401 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5461 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5521 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5581 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5641 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5701 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5761 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5821 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5881 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     5941 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6001 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6061 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6121 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6181 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6241 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6301 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6361 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6421 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6481 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6541 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6601 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6661 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6721 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6781 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6841 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6901 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     6961 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7021 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7081 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7141 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7201 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7261 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7321 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7381 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7441 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7501 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7561 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7621 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7681 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7741 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7801 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7861 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7921 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     7981 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8041 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8101 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8161 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8221 caaatgagat ccgagatctt ttgacagaag agatcgagcg aaaaaaacaa gtattagaag
     8281 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8341 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8401 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8461 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8521 tttcagacga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8581 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8641 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8701 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8761 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8821 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8881 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     8941 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9001 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9061 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9121 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9181 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9241 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9301 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9361 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9421 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9481 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9541 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9601 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9661 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9721 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9781 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9841 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9901 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
     9961 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10021 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10081 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10141 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10201 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10261 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10321 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10381 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10441 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10501 gctcgtattt atggatacga ccagttgcca tctactcttc caagtggaga aacggttgct
    10561 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10621 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10681 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10741 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10801 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10861 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10921 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    10981 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11041 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11101 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11161 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11221 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11281 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11341 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11401 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11461 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11521 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11581 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11641 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11701 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11761 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11821 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11881 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    11941 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12001 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12061 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12121 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12181 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12241 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12301 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12361 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12421 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12481 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12541 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12601 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12661 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12721 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12781 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12841 agaaacttta caaattacta aactatcaga tataatgtat tttattatac atctttaaaa
    12901 gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg
    12961 tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag
    13021 gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta
    13081 tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg
    13141 ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca
    13201 atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga
    13261 agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt
    13321 tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa
    13381 tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt
    13441 tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta
    13501 tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta
    13561 tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa
    13621 atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg
    13681 aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt
    13741 gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa
    13801 tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat
    13861 attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag
    13921 atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca
    13981 ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga
    14041 aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag
    14101 aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc
    14161 aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa
    14221 ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact
    14281 acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt
    14341 ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg
    14401 aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat
    14461 caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc
    14521 gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg
    14581 agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta
    14641 tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt
    14701 ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta
    14761 gatggattcc aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa
    14821 acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct
    14881 ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt
    14941 atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc
    15001 gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc
    15061 ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac
    15121 ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg
    15181 gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt
    15241 actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt
    15301 gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt
    15361 atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc
    15421 ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct
    15481 atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca
    15541 ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca
    15601 tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca
    15661 ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag
    15721 aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt
    15781 tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg
    15841 attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa
    15901 aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta
    15961 cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc
    16021 ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac
    16081 aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta
    16141 gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag
    16201 gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca
    16261 aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa
    16321 aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag
    16381 cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc
    16441 ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg
    16501 tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca
    16561 cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga
    16621 tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga
    16681 gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac
    16741 ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg
    16801 aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact
    16861 ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga
    16921 tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac
    16981 aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa
    17041 tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat
    17101 tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt
    17161 agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt
    17221 tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc
    17281 aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac
    17341 agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga
    17401 atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca
    17461 gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa
    17521 acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat
    17581 catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc
    17641 gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg
    17701 cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca
    17761 gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat
    17821 ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt
    17881 atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt
    17941 attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc
    18001 ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa
    18061 gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca
    18121 agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg
    18181 gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg
    18241 ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga
    18301 cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac caggattcga
    18361 acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc
    18421 gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat
    18481 ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt
    18541 gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat
    18601 cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga
    18661 agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa
    18721 aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa
    18781 atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc
    18841 aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt
    18901 cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag
    18961 atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc
    19021 aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg
    19081 ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc
    19141 cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc
    19201 tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga
    19261 aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga
    19321 taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg
    19381 ctgggacaaa agcgtttaac tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa
    19441 atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgcc gtttatacgt
    19501 gtttagagcg tgggacaaaa gtgttcttta cttttgtccc acgctctttt tagtgagatt
    19561 cgttgcatcg tttgtccaaa ttattttccg acagcaattg gactgttcgg aatcgtgaaa
    19621 ccagaagctg tcaattcttt gacataggag ctcaagaact cttcttttaa ggcatattgt
    19681 tttccgttca atgcgtagca gaccgtacga atcgcaaaat tgctgtttcc aagatccact
    19741 aatccaaaga tagaaggttc agtttggagc tcttcttggt atttttcagc taatgtacga
    19801 tttactcgat caatgatctc atagattttg tcatatcctt cttcaggaac aatccgaata
    19861 tctaaaagta cttgcatatt tgcacgagag agattgctga tcgtggtgat gtttcgattc
    19921 gggatgaaat ggacagtacc atccgttgct ttcagctgaa gggtacggat accaacagat
    19981 acgacggttc cttcaatcgt taagtttgct agcttgatgt agtccccaac atccatctgt
    20041 tgttccatga taataaaaaa tccggtaata acatcactca tgaacccttg ggcaccaaga
    20101 ccgattgcca cacctgcaat tccggcacca gccaaaagtg atccgatcgg cacaccgata
    20161 gcagataaaa ttgcatagat aaaaaagaaa ccaagggtat aatgaaaaat agtatttaaa
    20221 agactatgta aagtctttaa gcggctctcg tttagaatgg cttttttaga atactgatta
    20281 aaagacttat caatcagata ttttcccaca cggtgaagaa tccaaaaaag gacgatcaaa
    20341 aaaatcagat ataaactttt ttggataaag gcagccaaaa tggcgtccca atcaatcccg
    20401 tcccaaaacc gctgaaacgc atttactttg ctgacagctt gatctgtcaa tgtctcgcca
    20461 gaagagtctg tcgtaccggc agttgctaat aatgaaaaca taaaatcctc ttttctaaaa
    20521 tttgattctt tctgtattgt accaagaagt tttcaaaaaa ggaaatagca aaaataacta
    20581 taatgaagga aaaaagctga aaataaaatg acaaataata tggataatga taaaatgtgg
    20641 agaaaataac gaaggtggta agtatatgca aatcgattgg aacaatctag gtttttctta
    20701 tatcaaaact ccttggcgtt ttattgccaa atggaaagac ggacaatggg aagaaggaga
    20761 attgacagaa gataattacc tgacgattca tgaaggctct ccagcgcttc attatggtca
    20821 gcaatgtttt gaaggtctaa aagcttatcg tcgaaaagac ggcaaggtca atctctttcg
    20881 acctgaacaa aatagtcgac ggatgaatca aagtgctaga cgattgctga tgccaatggt
    20941 ccctgaaaag atgttcgttg atgctgtcaa gcaagtggta aaagcaaatg aagattttgt
    21001 gccgccctat ggaacaggcg ctacattata cttgcgccct ttactgattg gggtcggtga
    21061 aaacattggt gtccatccag ctccagaata tttatttgtc gtcttctgta cgccagtagg
    21121 agcgtatttc aaaggtggac taaaaccaac gaattttatt gtatctgatt acgatcgtgc
    21181 tgctcctaag gggactggtg cagcaaaagt cggcggaaat tacgcagcga gtttacttcc
    21241 aggaacagaa gcaaaagaac tggattactc agattgtgtt tatcttgatc cagccacaca
    21301 tacgaaaatc gaagaagtag gtgcagcaaa tttctttgga attacaaaag acggaaaatt
    21361 catcactccg caatcgccct ctatcttacc aagtatcacg aaatattcct tgttgcagtt
    21421 agctgaagaa cgactaggac tgcaagcaat tgaaggagat attttcattg atgatctaga
    21481 tcaatttgca gaagctggtg catgtggtac agcggctgtt atttctccaa tcggtgggat
    21541 ttatcacaat aacaatcttc atgtatttta tagtgaaacg gaagtaggac cagtaacaaa
    21601 acggttgtat gatgaattga caggtattca atttggagac atagaagcac cagaaggctg
    21661 gattgaagtc gtggaataat ttcaaagtaa tgagttgaaa aaaacgtaaa aagaaaaaat
    21721 caggaagaga ctttaaaaag ttttttcctg atttttatat gttttacttt aaagaatgca
    21781 acaaaagttt atgaaagatg gacacctttg tcaacgtaga taatatcacc gatgattccg
    21841 ctggaaagtg ggctgattaa gaaagcacaa gcattaccga cttcttcgat cgtcacacct
    21901 tgctgatcag gcgtacgaga ttcagataac tggatcagtt tttgatagtc tttaacacct
    21961 gtgacagcta atgttttgat tgccccagct gagattccat tgacccgaat tcctttaggc
    22021 gagaactcag ctgccaaata acggatagca gcttcaagag aagctttcgc aatacccatc
    22081 atgttataat tcgggattgc gcgttctgaa cctagataag tcatgctaac gatcccgctg
    22141 tttggtgcaa gaaacggttg tgcgtatttt gatacagcaa ttaaagagta gctgctgata
    22201 tcttgagcta attgatagcc gtctctggaa atatcagaca catttccgga caattcttct
    22261 ttgttggcgt aagcaactgc atggacaagg ccgtggatct ccccagcata ttcaccgatg
    22321 gcattcattg ctttttcgat gctttcgtcg ctagaaacat cacattctac agtgaatgct
    22381 tcttcgccga ccaatttgac taatgatttt ttcattcgtt cattttggta agtatagata
    22441 atttctgccc cttgtttcaa cattgcttca gcacaacccc aagcaatgct ttttttgtta
    22501 gcgacgccca taatgacgat tttttttcct tctagaaatg acataatgac tctcctttgt
    22561 ttctgataaa aatttgatag tcgtaaaaaa atagagaata atagctaaaa taacttcttg
    22621 atttcttaat tattagtcaa aatgttttga ttgtcaaact attttttctt aaattaaaaa
    22681 aagattgcta aatatttagg aatatgatac tttgatagtc aaagtaatga aaatggcaat
    22741 tgcctgaaag aaggagttta attatgtctt acgtattatc tgctactgaa gtaatggaat
    22801 tgattcctaa tcgttatcct atttgttata ttgattatgt agatacttta gaaccaggaa
    22861 aaaagatcgt tgcgacaaaa aatgtaacaa tcaatgaaga ttttttccaa ggtcactttc
    22921 ctgacaatcc agtgatgccg ggtgcgttga ttctagaaac actggcacaa gcaggttcga
    22981 tacttatcct aaaatcagaa gagtttctag gaaaaagagc ttatatcggt gggatcaata
    23041 aggccaaatt ccgtcaaaaa gttgttccag gagatgtgct gaaatgtcat tttgaaatca
    23101 ccaaaatgaa gggatcagtc ggtacagcgt tatctgcagc atatgtagat gataaaaaag
    23161 tctgtgagtg cgagttcacg ttcatcgtca accaaaatgc ttgactataa aacctgatat
    23221 agcaaaacaa aaggaagctt acttgctttt tatgtaaaaa gaagacgcct gaatcctagc
    23281 atacgttgtc atctatgcag gattcagacg ttattttttt atttttgttt tgcttcttgc
    23341 agtatattga ttttatcgat ttttacttct tcttttggtt tgtctgaact atctgtttcg
    23401 atatttgcaa tagcatccac gacatccatt ccttcaatca cttggccgaa gacagtataa
    23461 tggccatcta gtgaaggata accgccgttt ttataagcgt cgatgattgc ttgaggatag
    23521 tcgtctttta aaagaccatc atgcatatca tctgtatttt gaacgataaa gaactggctg
    23581 ccgttactgt taggatcttg tgagcgagcc atagataacg cacctcttat attataaagg
    23641 tggttgttga tctctgtttc aaatccttct ccccaaatgc tttcccctcc agtgccatcg
    23701 cctttaggat cgccaccttg gatcatgaaa tctttgatga cgcgatggaa agtcagtccg
    23761 ttgtagtaac cgtcttttgc atgtgtcata aagttttcaa ccgcttttgg tgcgtgttcg
    23821 ggaaataatt tgattttgat tgtgccttta ttcgtcacca tttcgaccag atcttcattt
    23881 tcggcgacat ctttcgtcaa ttgtggtaat tccaatgcgt tcaaatcgac gctactggca
    23941 gtcgtgcttt ccgcagtaga tgtacttttt gaactttctg tagacgaact agaagaagtc
    24001 gtgttgtttg cagaacaacc agcagcaaac agcaatgctg ctgctaaact aatacttaaa
    24061 ataactttat ttttcttcat aactgttcct ttccaattta ctattttgtt atctttaatg
    24121 ataacaaaat agagggaaaa tacctactta aattattgga atttatcatt tattttaaaa
    24181 gaaaaagaaa aggtgtagac catttacttt gtgtttgcta taatgtaagt ggatacaaaa
    24241 acgaaaagat gcaggaggaa atggtaatat gggaaaatta ggaatctcga tttatccaga
    24301 acggtcaaca tttgaaaagg acaaagcgta cttggattta gctcataagt atgggtataa
    24361 acgggttttt accagtttgc tgcagatcaa tgatgataaa gaaaaagttc tttcagaatt
    24421 taaagaagta gttgattacg cgaatagcct aggcatggaa gtaatggttg atatcaatcc
    24481 agcgttattt gaacagttgg agatttctta cgatgactta tcttttttcc ataagatggg
    24541 tgcctacggg gtccgcttag atatcggatt cacaggagca gaagaagcaa aaatgacgcg
    24601 caatcctttt ggtatcaaaa tcgagatcaa tatgagttcg ggcacgaatt acgttgacaa
    24661 catcatgagt tattcgccaa acacggataa tttattaggt tctcataatt tttatcctca
    24721 tcgatactct ggtttaggat acgaacactt tgttttttgt tcagaaaaat tcagaaaata
    24781 taatttgaat acgatggcat tcgtcaattc gcaatctgct gagtttggcc cttggcctac
    24841 gcaagatggc ttgtgcacgc tggaagacca ccgagatctt gaaattgcga cacaagtgaa
    24901 gcatttaatc cttacaggat tgattgatga tatttctatt ggaaatgcgt acgcaagtga
    24961 agaagaattg aaagaaatgg cagaagcatt taacgcggat tatccaacat tgaaagtaga
    25021 tacggaagaa ggtattacag aaaacgaacg aatctgcttg tttgataacc ttcatagtta
    25081 tcgaggggat cgctcagaat atatccttcg ttcaacgatg actcgtgtat actacaaaga
    25141 taaagatttc ccgcctcata atacgcgaga tatgcatcat ggggatgtac tgattgacaa
    25201 tgaagggtac ggtcaataca aaggagaaac acaaatcgcc ttgaaggata tgaaaaacga
    25261 cggacgtgtc aatgttgtag gaagaatctc cgatgacgaa ttattcctcc ttgacttttt
    25321 gaaaccatgg tcaagcttta aattaattga aaataaaaaa tagataatta gttattgcag
    25381 aaataatccg ctagaaatag ccagttattt ctgctttttt attttgtttt tttatag
//
LOCUS       DACDCV010000033        25351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDCV010000033 DACDCV010000000
VERSION     DACDCV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.033"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..3175)
                     /locus_tag="IV041_001309"
     CDS             complement(26..3175)
                     /locus_tag="IV041_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8382113.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3189..4406)
                     /locus_tag="IV041_001310"
     CDS             complement(3189..4406)
                     /locus_tag="IV041_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8382114.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4396..5991)
                     /locus_tag="IV041_001311"
     CDS             complement(4396..5991)
                     /locus_tag="IV041_001311"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8382115.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6116..6901
                     /locus_tag="IV041_001312"
     CDS             6116..6901
                     /locus_tag="IV041_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8382116.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7610..7969
                     /locus_tag="IV041_001313"
                     /pseudo
     CDS             7610..7969
                     /locus_tag="IV041_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8264..9397)
                     /locus_tag="IV041_001314"
     CDS             complement(8264..9397)
                     /locus_tag="IV041_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8382117.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9388..10056)
                     /locus_tag="IV041_001315"
     CDS             complement(9388..10056)
                     /locus_tag="IV041_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382118.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(10512..11498)
                     /gene="lacD"
                     /locus_tag="IV041_001316"
     CDS             complement(10512..11498)
                     /gene="lacD"
                     /locus_tag="IV041_001316"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8382119.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(11511..12437)
                     /gene="lacC"
                     /locus_tag="IV041_001317"
     CDS             complement(11511..12437)
                     /gene="lacC"
                     /locus_tag="IV041_001317"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8382120.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(12450..12965)
                     /gene="lacB"
                     /locus_tag="IV041_001318"
     CDS             complement(12450..12965)
                     /gene="lacB"
                     /locus_tag="IV041_001318"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8382121.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(12984..13412)
                     /gene="lacA"
                     /locus_tag="IV041_001319"
     CDS             complement(12984..13412)
                     /gene="lacA"
                     /locus_tag="IV041_001319"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8382122.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(13432..14205)
                     /locus_tag="IV041_001320"
     CDS             complement(13432..14205)
                     /locus_tag="IV041_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8382123.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            14370..14849
                     /locus_tag="IV041_001321"
     CDS             14370..14849
                     /locus_tag="IV041_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8382124.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            14906..15208
                     /locus_tag="IV041_001322"
     CDS             14906..15208
                     /locus_tag="IV041_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8382125.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            15230..16687
                     /locus_tag="IV041_001323"
     CDS             15230..16687
                     /locus_tag="IV041_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8382126.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(17280..18032)
                     /locus_tag="IV041_001324"
     CDS             complement(17280..18032)
                     /locus_tag="IV041_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8382127.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(18247..18483)
                     /locus_tag="IV041_001325"
                     /pseudo
     CDS             complement(18247..18483)
                     /locus_tag="IV041_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(18493..19451)
                     /locus_tag="IV041_001326"
                     /pseudo
     CDS             complement(18493..19451)
                     /locus_tag="IV041_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(19657..21339)
                     /locus_tag="IV041_001327"
     CDS             complement(19657..21339)
                     /locus_tag="IV041_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8382128.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(21350..21673)
                     /locus_tag="IV041_001328"
     CDS             complement(21350..21673)
                     /locus_tag="IV041_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8382129.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(21696..23102)
                     /gene="lacG"
                     /locus_tag="IV041_001329"
     CDS             complement(21696..23102)
                     /gene="lacG"
                     /locus_tag="IV041_001329"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8382130.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            23569..24576
                     /locus_tag="IV041_001330"
     CDS             23569..24576
                     /locus_tag="IV041_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8382131.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(24970..>25351)
                     /locus_tag="IV041_001331"
     CDS             complement(24970..>25351)
                     /locus_tag="IV041_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8382132.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         7550 a         5284 c         4191 g         8326 t
ORIGIN      
        1 gtacaatttt cttaactctt ctcgtttacc tcaattcatt ttcaagtgga atcaactcat
       61 catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt
      121 ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag
      181 gaattggatc ttcacctggc atatattgca gattagaagc atgtaattct ttctcagaaa
      241 caaaccactc attggaaaag gcatgaatca aattatcact tgcttgtgta aaatagcgac
      301 gtttaatgac aaagatatct tcattactat ctaaagtccc cacatcaaat gcatttaaaa
      361 tttgtcggta aacttctttg actccttctg ttttattcaa tttagccaaa gctttttcaa
      421 tttcttctcg tgcatctgaa ctattcgcag cataagttcc tagtaattga tcaatataag
      481 tagaatcaat atcatacaac ttagtggtat tttcactata aaattcgatc gttgaaaaat
      541 cttctccctc tttttctggt tcttcctcca tcaatgaacc cttcacggtc tcgtacacgc
      601 caatttgttc ttccaaaagt tgaatttgat tgttcaaagt tgaagattgc accatgtcat
      661 cattgtaatc atcataagtc actaacgatt catacgcacg attcaattct tgaaatgctt
      721 tgacgtactc gatacggatt tcaagtggcg tatgttcatc aatggattcc atggatgaaa
      781 cctcttttaa cttaccatat gcttgcttga atcttttttt ggattcttgg taacttggat
      841 aaatcaaacc cgattcttct ttagcttctg aatacagttt tgtcgcatcc tcgacattct
      901 tctccattgt cgctggcttt cggaaagtaa caattagtcc ttttgttttt tcaggatacg
      961 tacggttcgt acgtgaaaag gcttggatca atcctgcata ctctagatta cgatccacaa
     1021 atagggtttg aatcgtaggg gcgtcaaaac ctgtcaatag acgatccact acgattacta
     1081 aatcgatttg tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa cgattattga
     1141 tgtctccgtt ataacgctca atgtctgcta gtgaccaggc agtatcataa tactgattat
     1201 attcctcaat aattttttgc atttcttctt gttgggcact tgaatctcgg ctattttctt
     1261 ctaatgaata ggtaatagcg attcgtggga aatcaggatc ctccatcgtt cgtccttcac
     1321 ggataggctg attgggaaac tcttttatta accaatcaga ttctttcgtc atctctttga
     1381 tggcccgata atactttttg gccatatcaa tggaactcgt ggttaagatc gcagacttcg
     1441 ttggacggcc atttcgaaaa tcaaattttg tataggcatt gtctggacgg aacattttat
     1501 gaatgacttt ttggatatgt tcctctgctt caaaaacagt agggtccaaa taactttctt
     1561 ttttgacaga ctccatttga tcgatcatac gattgatctg ttccggtgaa tactctgcat
     1621 atgtttctac ttcttttaat ttccgataga ttttattctc taatgaagtc ggctccacgg
     1681 tgttttcatg ctctacttga aatcctaaaa ccgagccatc ttctaatgca ttcttgatgg
     1741 tataggtgtg caagacctct ccatactgat catgggtcgt acgtgccaat tgccctttgg
     1801 cttgtttccg attctcaggg aaaatcggtg tacctgtaaa gccaaaccaa gtggatttcg
     1861 gaaacatttt tttgatttct tccatatttt cagcacttaa cgctcgatga cactcatcaa
     1921 ctacaaacac gatatgttgc cccattaatt tttggaagcg attggttcct tttttctctt
     1981 cctgtttctt tgcatatttc aacgccgcat ctagtttttg acgggttgta atgatcacaa
     2041 cattcgcatt ggcatctgcc aacagagttt cacttaactc tttggcactt cctgttccca
     2101 cgatcaagct attcgctttg gcattaccag aagaaacccc agtattaaac tccgaagcaa
     2161 atttcgtaaa ctctgtggtg gtctgattat ctaaatcttt gcggtccact aacatgatcg
     2221 ttcgatcgat ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta gtcaacgtct
     2281 ttcctgaacc agtcgcatgc cacacatacc ctgattgatg tttatttgcg gcaataaaca
     2341 aggcttgaat cgcatgcact tgataaggat gtaacaccat gagggtttta ttgtcttgat
     2401 cttcactgac aatcgtataa tccgcaatca agcgatgggc atctgggata tttagcactt
     2461 gtttgcaaaa ctcatataag ttttctacct ttttattatc tttcgttcgc caactaaata
     2521 aaaacttagg atgcaaatcc ttcggtaagg catttgcaaa ataccgagtc gtttgttcat
     2581 tggaaaccac aaacaactgt aaggtcgaaa agatattgtt ccgaaacatg ccttcttcag
     2641 catatttttt gatttgatta aacgcctgat aaaaaccatc ttttgcagtt acttgtttta
     2701 gctcgatttg gataatcggt aagccattga tcaataaggt gacgtcaaaa cgtcgatccc
     2761 gcgcttctat gttacttcca cgttttgcaa tttgatggac cacttcataa gtagagattc
     2821 ctccaccaat gtcttgattg gaataaagga taagtgagac agaacccaat tgcggatttt
     2881 cccgttcaat cgtgatccga gccatcccat tttcaccttt taaccatttc gcagcatcaa
     2941 atggggtttt cgtccgtagc aataattcgg ttttgatcgt ttcaaactct ttatcggtca
     3001 aaggagaatc attcagttct gcttgattat ttgagatgat tttttgacgt aaattgctcc
     3061 aaagatcttc ttctgatttt agatccggac gatacgtcca ctgattatgt ccttccccga
     3121 ggacacgaat caactgttct tcgacttccg cttcctcacg gtgactaatt ttcattcttc
     3181 ttccctccct aaacaaacat cttttgcaag aagccttttt tcgtttcctt cagcaaatct
     3241 aacttacgtt gatgaagagc gatcgtatca tctagctgct tgaagaaggc accaattttt
     3301 tgttgttcat ttatatccgg tggaaactga aatgtccttt gttctatacc acctttagtc
     3361 acatgtacca ttgtcgttcc gttagttgat tctttaattt tctctttatc agtcaatagc
     3421 caagtatata gatactgttt gtccaattcc ttagataaaa atttaagttt ccaaatatga
     3481 taatggtaga ttacacgttc ttcattccac atttcaggac cgaagcttgt tgcccataaa
     3541 tataagagat ctcctttatt agcatacttg tcttcctcta attctaagtt tgaataatac
     3601 catctatcat tagtattaaa attacctact cttaaaaccc tatattttcc agaatcaagt
     3661 aattcttgtt gtttaaaagc tctaccattc aaaaaaacta ccacttcccc caacttccgc
     3721 tcttcccaat ctcccgtaaa tcctggaaaa cgaacttctg gcacttttgc tccgttttta
     3781 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa ttcgtgctgg
     3841 tgaagcatga tggtgtgatc aagttgtttg aaaaatgaac cgatcttgat ctgttcgtca
     3901 taagttggaa gtgagatagg tgtcttttta ccactatcaa ttgtataggt tccgacggtt
     3961 ccaatgcctg caacgtcacg gatatacttc tgaattttgt tatttatgaa cgagtagtaa
     4021 aagaagtttc cgtcaatttt atgctcattc ttgaaccaaa tgatgtttcc atccttaaag
     4081 taaattgggt tatcatcagt gattaataag gggacaccta ttgatccgac accagttaca
     4141 agcaagtctc cttgagaaac tttacctgaa tttttcgaat attcattata tttttcttct
     4201 gaaatgtaaa gataatcgga tggttcctca ttttttgcag ctgagacaat atcacgagca
     4261 cgtaaaaatc taatacctga gttagtccaa tctgattgat gaattcgctt cacagaagta
     4321 acatccatca tatctcctaa cttacgctct tcccaatctt ccgtaaaacc aggaaaccgg
     4381 atttctggtc gtgtatcatt gctcatgttt gaacacctcc agcgcacctt gtaaccacgc
     4441 ttcattttca ggtgttgttt gcagggaaga gatagcttct aaaagttctt tttctagcac
     4501 ttgctgttct tgtcgtatct tcttgatgtc atttccgaca tgaaccatat caattggttc
     4561 ttcttcttcg aaagtatcga catagcgtgg gatatttaag ttatagtcat tttctttgat
     4621 ttcatcaaag gttgccaaat gagcgtattt ctcgacatct tttctttcag cgtaggtttc
     4681 gaggattttt tgaatgtttt cttccgataa tttattctga tttttccctt taacaaattc
     4741 acgactagca tcaatgaata atacatctcg agtttgacga ttcttcttca atacaatcac
     4801 cgtcgttgga atcgatgtac caaagaacaa gttcgcaggc ataccgatca cagcataaat
     4861 actgccgtct tctaataatt tttgacgaat gaccccttct gcagcaccac ggaataaaac
     4921 cccgtgtggc aagacgatag ccatagtccc tgtttccttc aaatgataga aaccatgaag
     4981 aagaaacgca aaatctgctt tggattttgg cgctaatttc ccgtagcgat taaaccgaga
     5041 atcatctaag aaggtcgtat ctgctgacca attagccgaa taaggtggat tcatcactac
     5101 tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta ttcaacgtat ccccattgcg
     5161 gagattcatt tcttcggcat ctaccccatg taaaatcaag ttcatttttg ctagattgta
     5221 tgtcgttgta tttagttctt gcccatgata tttaacgtta tctggatgag ttaagtagtt
     5281 acgtacattt aacatcaagg aacctgaacc catggttggg tcaaagacac taaagaatcg
     5341 tcgttctttt tgatccaacg tcacgatttg tgccatcata tcagaaacca tatgaggcgt
     5401 gtaaaattct ccggctttct tccctgcttc tgaagcaaat tgactgatca gaaattcata
     5461 cgcatccccg atcacatccc catcgtgacc taaaacttcc acgtcattca atttcttgat
     5521 aacttcggta atcgtcacat ttcgttgttg ctcatctgtt cctagttttt ttgattggag
     5581 atccacatca tcaaataacc cattaaattg attgtaggta gatgccaatt gcacaaaggc
     5641 tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct tgatccgcta agacgttgaa
     5701 aagatactct ggtgagatcg aataccccaa aatatcaacg attgtggcaa tcaggtcttc
     5761 ttttgattct tcatctgaaa gtaattcacg atacaacatt gtttgtttgc ttactgtgtc
     5821 atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac ggtcggataa
     5881 atatttatag aaaatcagtc ccaataaata atttttatat tctgacgcat ccattttact
     5941 acggagatta tctgccgcac taaataattt ttgatttaat tctgcaccca aaaagataca
     6001 ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact cccttttaga
     6061 aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga cgtatatgcc
     6121 taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag aaccggcatc
     6181 cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata aaaatttaaa
     6241 agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta tatcagtacc
     6301 tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt gtaataaata
     6361 catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg gcgaaaaaga
     6421 tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa aagtattgaa
     6481 tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg gaagtatttt
     6541 agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg ccggctctgt
     6601 atatcgagct gttgatttct gttatttagg aacctcacca attcttttac aagtgaaaaa
     6661 taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa caattagaaa
     6721 atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta tccccaattg
     6781 gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt taactgaaaa
     6841 tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac tcgatgacta
     6901 acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac aaacaaaaaa
     6961 actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt tggatttacg
     7021 aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca attactaaac
     7081 ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta gtttaaaagg
     7141 gaaaatatgc ttctatcatt acaagctcgt ttggtgtaag atgggtataa gtcatttatg
     7201 ttcactctcc ttgtatgctt tagcgggtat tacaatttga gtgtaacata aatggctttt
     7261 ttatttgtct cgcttaatta tacaaacggc gaattataaa taactcataa aaaatatttt
     7321 gactttcaaa atcacttttg ctaccagaag ttgaggcgaa gtgttttaag catagttgta
     7381 ccctagtttg accgttaaaa ataaataacg atcgctagag tgcaactatg tttgtttgcc
     7441 ctaccgtcag cgtcggcttg gcctctcaca tccattaccg tcgggattat ctccgttaca
     7501 tacccttaca aaatacgccc tgtaagcatt gcatgaaacc accgtgcatt tgcaccgtat
     7561 ttccgcccta gccgttcaaa tcggtcgcta ccaccattca gaaacacgtg cggtaatgga
     7621 aggtttggag gaagctatcc agaccactaa cgattgtccc tatcgtcgta cgtttcactc
     7681 agatcaaggt tggtcgtatc aaatgaaagc ttatagaaac aaattgaagg agtacaaaat
     7741 ctttcaaagc atgtctcgga aagggaactg cttagataat tcacttatgg agaatttttt
     7801 tggcttatta aaacaagaga tttttcacgg gaaagtttat aactgtttcg aggaattaaa
     7861 atcagccatc gatagctata tctattacta caataatgaa cggataaaac agaaattaaa
     7921 ctggcaaagt cccgtccaat ttcgtaaaac aaccgttacg gttgtgtaaa aatatcattt
     7981 tagagcatga aaaaaagagt ggatttctcc actccaaaaa agtctaactt ttgggggtca
     8041 ctacattttt gatccttttt atttgattaa ttatatccag ctggataata attttggggg
     8101 caacatttct atcttactat tatcttccta aagtagtaga ctttcttatt gggaaaaatt
     8161 acaacaaaaa ggagtggctt agttgacaac tcctttttgt ttacaaatca attggatatc
     8221 tataaattgt attttctaac gaattttata gacatctgat ttgtcaaatt aagctagaca
     8281 atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa tttaatgatt
     8341 ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca tccgtgtccc
     8401 taaagtccat tgttttaggt aagccatcct gcttgagccc atgcactcgg tactgatgag
     8461 aaaaaattga tgcctgtttc tcctgaggaa tgccaattcc ctgatctttt acttcaataa
     8521 tcacgctacc cttttggttt gaaatggtga caattaccag ttttccttca ccatatttga
     8581 ttgcattgtc aactaaattc gaaaccgccc gataaatcca acgatctcgt ccatgaatca
     8641 gggttggttg atcttcagta aacgcaaaca aaagattcgg ataattcatt ttgtaggcat
     8701 cacaaacttc cgcacagatc agtgaaacgt ctacgacttc cgtgctaaga tcctctgagt
     8761 tattggataa tgtcaaaata tcgtcaatac tgtcggtcat ccgatctaaa agcattaaat
     8821 ttccttgatc ggcatgttgc cgttctattt ccagattcgt acgcaaaata gcgatggcat
     8881 ttttctgctc gtgggtcaga taactattta atcgtcgata atcctccaaa tgaccatcga
     8941 acttatcctt tagcttatta aaagccgcat ttaatgcctc gtccttcaca gtatttttag
     9001 gaacttgatc aataaacgtt aaatcacgcg taatcgctaa gatttgatta ctgtaaattt
     9061 tgcgtaaaac caaccaaaga ataaacgtta ataacatgac ggcagaacaa ataaccagca
     9121 ccatcgctgg taaatattta aatagttcgt cctggtaagc tttatctatc atcgaaatag
     9181 tcataaattg attgttggaa gatttcgaag cttccatctg cgtctctgct tgaaccgtct
     9241 taccctcttc cataaaataa acatcttcac ctaaggtaga tgcgatactg attccctcca
     9301 aattatttct cagaaagaag aaactcacta aaatcacgcc tgtcaccaac agtgaaaaag
     9361 ccagcatcgt taacgtcatt ttaaaattta atagcatagc tggtacccct ttgctcgaat
     9421 ggtttttaag acctcttttt cagctgcttc agccagtttc ttttttagac gagccagatg
     9481 cactcgcaga accgacgaaa aaggatcaaa atcttcatcg tagacatgtt cagaaatttc
     9541 ctcagacgaa acaaccgctg gatgacgctg agcgatgcac agtaagatat caaattcctt
     9601 gggtttcagg accacctctg tcttttgata gctgaccctt cgacctaatg gatcaatctt
     9661 caaagcacca attgaaatct gcggcagcgc acgtccatga taacgacgaa taaccgcgcg
     9721 tattcgtgct tttaattcta gtaactcaaa gggtttgact aaataatcat ctgctccaaa
     9781 atccaagcct tttgctcgtt cttcaatctc atcacgtgca gtaataatga taactggact
     9841 ttcgacttct tcagcacgga gactttgtaa aacagataat ccatcttttt taggcagatt
     9901 taaatctagt agtatcccat cgtatgaatt gatgaaggcc ttctcttctc ctgactcgcc
     9961 atcataagct acatctacag aaatattctc ctgctctagg ccctttttta cggataaaac
    10021 cagctcacga ttgtcctcaa ccaccagtac acgcatgtat attcactcct catacggatt
    10081 tagtaattac acttatgctt tttagcatag tacacgctct tcttcaaagt ccacgttctt
    10141 tttgtgaaga tttgaagaaa ttcatacatt ctgttaaaaa acacataatt aagaatgatt
    10201 ttactgtttg aaaataccta gaatagaaaa agactggaac ctttcctcca gtctcactac
    10261 tttctatttg cttggttttc ttgtatatac ttttcaaagt tcgttggttt atgaccaata
    10321 agcctcgtta gaatactgaa aattgccatc aagtcattaa gtaccacatg taagaaaatc
    10381 tctctaaaga aaaacccctt cgttttaact cacttactat tttagaattc gttcccctaa
    10441 taatgcagca tgattttacg ctttatgtat aaaaaaagac tgcaagcaat tttgcttcac
    10501 agtctgattt cttatgatag tgagcttgtt aacacttcca ccggtgtagc agtttctttc
    10561 aatacggcat ttaattcttc aatattttta cgcccttgcg tattcatcca ctccatcgct
    10621 gcttcttttc catcaacgat atagctttct acaccatctg cccaagtcgc acggccgcaa
    10681 agtacaccat tgaaggttga gccggcattt ttcgcgaagc gcagtgtttc ttggaacaac
    10741 tctgctttta caccggcgct taggaaaata aatggcaggt tagtggcttc agcctgttcc
    10801 ttgaagaagt tagccgcttg tgcctttgtg tacacgactt cgtctgtccc atagccttcc
    10861 acataattca tattgacggg tacttccacc tttaacacat ctacgccata acgtggatca
    10921 gaaaattcct tcatcatatc gttgacttta tgaggtttct taatggcgta ttctttcgaa
    10981 gtcgcatcag agatgttcgc gtcataagaa acaagctcca agaagaaggg taagttttca
    11041 gcctgacatt ctgagccgat tcgttcaata aaggcatgtt tttgattatt gatttcttct
    11101 gcttcatcga catcatagta aagtaggaac ttacaagcat cggcgcctgc ttcttttaaa
    11161 cgtttaactg accagatcga taataaatct ggtaaacggc cgggagttga cgcatcgtaa
    11221 ccagtttttt cataagctaa cagcaagccg gcattttttg ctctaacgtc agctgctggc
    11281 aagccatatt cgggatctaa taaaatcgat gaggcatatt tcgttaattg tgaagaaaca
    11341 atttctttaa attcaacgat ttctgattct tctgcttcgc tgcccttgaa ttttgtgatc
    11401 atcttcttca aagccccgcg ttgatcaatt gctaaggccc caatcactcc atcttcattg
    11461 catagttgct tgatatattg acgtttttct tcgttcattt ccgtatcctc ctacactttc
    11521 tccactaaga tttgatcata tagtttttga taattggcta attggacata gccagtcgtc
    11581 gcttcctgag cattcagcat tcctaaaaca ttaccctgtt ttaacacggc ttcatccgaa
    11641 agaccttgtt gaatcgcagc ggtcaatccg gcaacggtcg catcaccaga gccaactgga
    11701 ttgacgacat caatctttgg aattgtcacg caataaaaag tctcgtgatg tttggcaaag
    11761 gctccgtcgc cccccattga aaccactatc cattcaattt catcaaacat cgaatctgac
    11821 aaggcggctt ttaaatctga taattctgtt gagatttctc gtcctagcag accagccagc
    11881 tcttcggtat tgggttttat tagcttaggc tttacactgc ctcgcaacac ttcccttaat
    11941 gctgtacctg aacaatctaa aacgacaggc tttggctgac aaacttgcag catttgtcga
    12001 taaaaatcag caggcaatcc ttctggcaag cttcctgaaa aagaaatgat ttcggcttta
    12061 acagctaatt tttcgaaatg ttgcaagaac gcccttgctt cttgttcagt aataacaggt
    12121 ccgttttcta ggatttccgt ctgttttccg tcatgtaaaa cagcgatgca attacgtgtc
    12181 tctccgctga taggtaaaaa gtcatgagca atctcatttt gagtcaggtc ctttttaatc
    12241 tcttcaccta aaaaaccgcc gattaaacca ctagcaatca cctccgtatc taattgcttc
    12301 aagatacgtg tcacatttag gcccttaccg ccagctgttt tgcgaacatt tcttactcga
    12361 ttgactgtgt cgagcttgaa ggtttctaat ggataagaaa tatcaatcga tggattcatc
    12421 gttattgtta aaatcatttg tccacccgct taatcgtggt attcgccacg atcccatttc
    12481 tcgataaatt catcgaagaa atgcggatca gactgcttgt cactggcttt ttcgacgtga
    12541 tcgattttgg cgatcaattt tttgttttct tcagtttctt gataatcggc attgatgaag
    12601 gcctctaata tgtcacagat caaaaattct cccgtaatcc gagcgccaaa gccgatcaca
    12661 ttggcattta gttgttcctt cgcatagata gcagtcgtca tatcgcgaac cagcgcactg
    12721 cggataccag gtactttatt gacagcgttg ttaatcccaa cacctgttcc acacaaacaa
    12781 actcctagat ctgcttctcc gctagtcacg gcctctccga ctttttttcc gtagatcgga
    12841 taatgggttc tggtaaagtc ataggtcccc atatccaaca cttcatgtcc ttttgatttt
    12901 aagaaatctg atactgccat ttttgtatct gtgacgatgt ggtcacagcc aattgcgatt
    12961 ttcatttcat tcatccttcc taattacgcc attttattta acatgtcgac acgtacttga
    13021 tgacgaccac catcataatt tgcttgtaga aattccttaa caatattcaa cgccaactca
    13081 tcgccaacaa tttttgatcc aagtgtgatc atccgagaat tattatgttt acgggtcata
    13141 tacgcactgc gctcatccga aacttctgct gctaccattc ctttaacctt tgttgccgtg
    13201 ataaagctgc cagcgccgta gccatcaacc gcgattccta aactaccttc gttttcattc
    13261 agatcatttg cgatcgccaa agttgattcc acaaaatctt ctgaagccgt ttcactctta
    13321 tctactactg taataccttc tttgatcaat tttttcttga ttaattcctt taattccatg
    13381 ccgtcactgt ctgaaccaat tactacacgc atttttgttt cctcctattt tttactgaat
    13441 gattttagtg aattgctgat aataactctt cagctcggct gaaagagtag aatccgtaaa
    13501 taatttgtcg atcttgcgca aatcatagaa attataaaaa tcgctatggt cgaatttgtt
    13561 tgcatctgaa accaaacatt tttctgccgc attatctaaa acaatttttt gcaaggtacc
    13621 ttcttcaata ctgaaattcg aaacctccga acccttcaat ccattcactc cgacaaaagc
    13681 cttcttaata ccgatttttt gaacagcttc attggcaatt gaaccgacaa aggctccagt
    13741 cttttcccga tatagcccac cgatcaaata cagttcaaat ctttttattt cacttaatag
    13801 attgaaaacc ggcagactat tggtaacgat tcggagagat gacggcttga tgtatcgtgt
    13861 cattaattcg atcgttgtac ccgatccaag aaaaatcgtg tcgtcttctt gattgatatg
    13921 agctgccgca tttttagcga tgtattcctt ctcttttgta tgcagcttct ttttttcagt
    13981 atgagaaagc tcatttgctt ctatagaagt cgacatttcc agataatcga ttttttgtgc
    14041 accgccatga acgcgtaaaa gctttttctt ctgcgcgagt tcctctagat ctcggcggat
    14101 cgtcatatct gaaacattca gtacttcctg aataaatttg actgttatcg tcccatagcg
    14161 atcacacatc gctaagatcg aacgctgtcg ttctgttttc agcatattct gtacctccac
    14221 gaccttataa taaaacattt acgaacattt ttcaacacat attgttcgtt tattttcgtt
    14281 tatgcgttga caatgatttt gtaagcggtt atactcaact tgtaaacata taaataaaca
    14341 taaacgaact aataaaggag ctgttttgtg tgaatgaatt ctttttcaaa gacactgtct
    14401 atgtttctga caagcagtcg caggaagaag tgttcgagga agtctatcaa gatctgttaa
    14461 agaaagaatt ggtaaccgaa gattttttgg agaacctttt agaacgggag cgtaattatc
    14521 cgacgggatt agatctctca cctgtctcta aaatattgcc agatatcgct atccctcata
    14581 ccgaaagtga gtttgtgcga accacccgta tcattccaat caaagtaaaa aatgaaatcc
    14641 ctttctacaa catgatctcg ccaagcgatc agatttccgc ttcatttctt ttcatgatct
    14701 tgaacgaaaa tggtgaagca caggctggca tgttggctaa tatcatggat ttcattaatt
    14761 ctgttgacga agaaaaactg ttttcgtttt tcaaattaga ggacacagaa gcgatttatc
    14821 attttttaga agaacatttt tatatataga agtctctgcc taaccgcagg ataaaaaata
    14881 aacttatttt aaggggagaa ttattatgat caaaatttta gcagcatgtg gagcaggcgt
    14941 taattcaagc catcaaatca aaagcgcttt ggaaactgaa ttgaaaaatc gtggctatca
    15001 agtcacctgt gacgccgtta tgatcaaaga tatcaatgaa gaaatgctgt ctcattatga
    15061 cattttcgca caaattgcca acaccgactt gggttttgac attaacatcc ctgtcgttga
    15121 tgcaggtgct attctttatc gtattcctgc tatggctgag cctgtttacc aaagtatgga
    15181 aagcgcaatc caatcattaa acaaatagga ctaaagaaag gaaaagcata tgaatacaat
    15241 tattgatatt gccaataaag tatttacccc cctaattaat ttaggtgcgg caccgttaat
    15301 gaccatcgta ttaactgtta ttgctttatt ttttaaagta aaaccgtcac gcgctcttga
    15361 aggcgggctc aaattaggga tcgccctgac tgggatcgga gctattattg gaattttaac
    15421 tactgctttt tcagacgcta tgacagcttt tgttgaacgc acaggtattt cattaaacat
    15481 tacagatgtc ggttgggccc cactagcaac catcacatgg gggtcgccat acacgcttta
    15541 ttttttacta attatggtga tcgtcaatat tgtcatgtta gcaatgaaaa aaaccaacac
    15601 attagatgtg gatattttcg atatttggca tttagcaatt gttggtttgt tcgcgatctt
    15661 ctgcggtgct aatctgttta ttgcttctgc attagttatt ttcattggtg tattaaaaat
    15721 ttggaactcc gacttaatga aaccaacctt caatgacttg ttaaatgcgc cagacagcaa
    15781 cccgatgacc acgactcata tgaactacat gatgaatccg attattatgg ttttcgataa
    15841 aattttcgac aaaatcttcc cttggttgga taaatatgat ttcgatgctg ccaaattgaa
    15901 tagtaaaatt ggcttctggg gatcaaaatt cgcgattgga atttatttgg ggatttttgt
    15961 tggattacta gcaggacaaa caccaacgca aattttctcg ctagccttta ccgctgccgt
    16021 ctgtttggaa ttgttctctt tgataggcgc ttggttcatc gcagccgtag aaccgttatc
    16081 acaagggatt accgattttg ccaataaacg actaaaagga cgtaccatca acataggttt
    16141 agactggcct ttcatagcag gccgcgctga aatctgggct gccgctaacg tattagcacc
    16201 aatcatgttg ctagaagcca tcatcttacc aggaaataaa ttattgccac taggcggaat
    16261 catcgcaatg ggtgttaccc cagcactatt ggtcgtaact cgtggaaaat taatccgtat
    16321 gatcgtaatc ggcgcgatcg aattaccatt attcttatgg tccggcacct tgatcgcccc
    16381 gttcgttact caaacagcta aagcagtcgg agcctttcca gccggcttga acgggtccgc
    16441 catgatctct catacaacga tggaaggccc gattgaaaaa ttcctaggtt atttagtcgg
    16501 taatgcttct caaggacaaa tcgaatttgt gatatacgct gccttagcat taggtgcata
    16561 ccttttaatc ttcatttggt acgctcgtca aatgaaaaaa cgcaacgctg catacgcagc
    16621 tgaaaaagaa gagaatgcat ctccggcaac ggcgcataat atgacttatg cagccgaagc
    16681 taaataagct aaaaacaccc cgaatgtctt tagacttcgg ggtattattt tacctataaa
    16741 aaataaatag atgtaaaatc catttcaaaa agcatagccc atctcttgcc acacccaata
    16801 aagaaattat caacgtttat gcttttttta actcctttaa gcttgccttt gttggtattc
    16861 ctcaacgatt ctttttgtgt tcttgtttgt tttgtgcttt ttgacttttt gttgtttaat
    16921 tgcgaattcc ttgatttctt ttttcctgaa tcgcttcgca tcccgcggtt atgcgtgcga
    16981 tttcatcatt gctttttctg atctaactcg tttcatgtca aagagcttca gctgacttag
    17041 tgtccatggt tgttttgata tcccttttcc tccttaataa agcggtgatt gatactgcta
    17101 ttttttcctt cacataaaca taacatccca aaaaaatcga ctatcttttt tcaaatggtg
    17161 tattcaagta ttcggaggaa ttagtaatga tgcaccctaa aaataacata attcgcttaa
    17221 attcaaggct accaaaaaac taccaagttc taattatcta gagtttggca gtttctcatt
    17281 tacacaacat atttttcttc tttttcgatt ttttcatagt gcttttgttc atcatcatcc
    17341 gttataacta ctgttacatc attcagatta taatatactg aaaaatctct tttcccaatt
    17401 ttcgaatgat caatcaataa aaagcgttca acagaattat tcagagcgat ctgttgagtt
    17461 tggccctctt caaaagtcga agtcataatt ttctgttcat ttaaagcatt acagctgaaa
    17521 aatgctttat gaaaattcat atcctgtagc gatttattcg tgatttcacc aacaaagcat
    17581 tgagtcgttt cccgcatctc gccacctaat agatagattt ttcgattccc ttttcgctta
    17641 tttaattctt caaaaacagg caaacagttt gtaacaatcc gcagtgaatc cccttgaatg
    17701 atttttgaca tgagctcgat tgtagttcct ggtccaagaa atatcgtatc accatcattg
    17761 atcatttcat tcgccttttc cacaatccgc tgcttttctt cttgcagcat aatctgtttt
    17821 tctctatgag aaagctccac atgccgataa aaatccttca atttcgctcc gccatggacg
    17881 cgttctaata atccttgttt ctccatttcg tccaaatcac gtcggactgt catatccgaa
    17941 accgaaaact tctccataat atcgctgact cgtactgtac cgttaacctc taataacgac
    18001 atgatcgctt ggaatcgatc cgtttttaac atttcatcgc ctcttttctt tttaagacta
    18061 tcaaattctt aagaaaagtg ctagaacaag ttttcttaag aagccttctg caggaaggaa
    18121 agcaaaattt ttcagaattt gcaagctgct attctaaaaa ataaaaccga acgagcatga
    18181 ctgtcatagt gacagccttg ttcattcgac ttttatcttt aataaatcat tttatttaat
    18241 ctgaagttat tgtgcaagtt caaaaacaaa gtagttaatg tctcctcgat atttcgaaat
    18301 cgaatactca acgggcagac gttctagcga aaaaccctgt gtatggaaat agaacattgg
    18361 atctccttct tccacatcta acaaatcgct gatcgtatca tccgctttga ttacttctag
    18421 ttttctggtg accgttttaa ttggataacc catttcttca aaaacttgat acattttttc
    18481 ttttctgatt tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac
    18541 ttccaatggt gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac
    18601 ggattgaata aaggtttcat ccatgtccct aaagtccatt gttttaggta agccatctcg
    18661 tctaagcaag ccgtttgagt gctcatttag ccctctttgc gaaggtgttc ctggatctgc
    18721 gaaaaagatg gcaatatcgt tcgagttact tatagatcgc cagttggaaa attcttttcc
    18781 acaatcaaat gtcatggatt taaacaagtg tttaggcatg gatttgaacc aatcatttaa
    18841 cctgttttcg atatcttcgg ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga
    18901 taaacactca accaatgtaa taacagcgct cttgtgttta actcccacaa tggtgtcgcc
    18961 ttcgagatga ccaaattctt tagtcaggtg tggatagaga ctgttccgtt catgaatcgt
    19021 tctgcgaaag gcctgttttc cacgcttttc ttgatgatta ttcggtttac gtttcccctt
    19081 cataggtaac gactgtgtgt caaaaaggcc ttttttaaac atccgataga gtgtacgaac
    19141 agaacaatca attggaaact cgccacggcc gataatcaca tcgggtgacc acccttgagc
    19201 taccttttct tgaacatatg ttgattgttc atcagataaa acggttgaac gtcgtccaca
    19261 acgagattta ttttgtttgt attgacggta ataatctagc acttttttcc ttgtttgaga
    19321 aacgaataaa cattataaat ggtttgtcta gagcgattta aactttgtgc gacaagagcg
    19381 acaggtttct cgatattgaa ataagactct atgattacta gttcgtctgt tgtaagatgg
    19441 gtgtaggtca tttgtactca ctccttatta attctttggt cggaactatt ttgagtgtag
    19501 cacaaatgat ctttttagtt gtctagctta attttacaat cgacgcctca aaaagaatac
    19561 ccgaaattta aacagcaaaa aaagatagcg cttaccattg tgtttaagat aaaacgctag
    19621 ctaagttggc tagcgcttta ttaatctaag caagctctaa aattgcttca atacaaaatc
    19681 aactgctcct ttaggatcac gtgttaaatt gatatattct gcacctttag ttgcaattac
    19741 ttttatccct aatttgtctg catctttacg gagatcatcc aaataggaat tcacttgagg
    19801 tgctagaacg attaaatcaa aatttttcat catatcataa tgagaaccat aagctcctgc
    19861 tttagcagaa agattaacac ctgtctcttt tgcgccctct tctaatgcat tagccaacat
    19921 tgcactcgtt ccggcaccag cacaaagtac taaaacgcgc tttccttcac caaccgatgc
    19981 ttttgcttca tcaactataa ctgccgcagt tgcaccaact tcttggattt cttcgaccaa
    20041 ttcttctttt acttcttctg cagcaatgtt ttttaactct tgttctacta attcagaatc
    20101 ataggcctta acaaaaggta aataaataat cgcatcgaca acaatcaata ctaccgctaa
    20161 tacaatcgcc aacacactaa acccggttcc taaaaccaag ccaatcggtg cgggcgtcgc
    20221 ccaaggcaat acggatataa agctattcat ccccaagaca tcgacaaaaa gtttgaatat
    20281 ccatacgtta gcgattggtg ctaataaaaa tggaattaga aagtacggat tcaaaataat
    20341 aggagtagca aatagtaacg gttcatttac cgcaaacgaa actggaataa atgaagcttt
    20401 accaactgct ttcaattgtt ttgatttagc gaacaacatg aacagaaaag gaacaactag
    20461 tgtcgctcca gtaccaccca tcgttccaac aaagtttcct agaccaactg ttaatacttt
    20521 tgaggcttgt gcaccgtttt taaataattc aaagttagtt tctacgttcg cataaataat
    20581 tgcagcaatc gctggttcaa caatcgatgg tccatgaaca ccaacaaacc agaacattgc
    20641 catggctccc caaatgattg caataccgac atatccatca gcagcactaa ataatggctg
    20701 caataattga attacacctg ctgcaaatga tgttccaaaa aattgacgaa ctcccgtatc
    20761 aatcaaaacg gcaataaata ctgcaattga aaaggggata atatctctaa acatctgtgt
    20821 gattgttccg ggaacttctt ttggcatacg gatcgtcaaa tctcttttaa cacagaattt
    20881 atacacattt acagtaacaa acgcagaaac gaaagctgct aataatccct ttgtccctaa
    20941 ataatcagta gctatcccac catcaatttg atcagctgcc atcattacaa aaccacaaat
    21001 tgacgctaac ataacagaaa cttcattaat ctgcttacct gaatcagata attgacggtt
    21061 cattgaatta gctaaacatc gagctgttgt agcaacaacc aaaagaccaa ctaatcccat
    21121 tgaataacca taaactttcc aaagccacgt cgagaatgcc tctggtaacg tcacgttaaa
    21181 tacttctgga acagccgcaa tcataataaa aatacttgaa aatagaatag ctggcatagc
    21241 agttaaaaag ccatcgcgaa ttgcccctaa ataaatatta cgtgagattt tttcaaaaaa
    21301 cggttttccc ttctcaattt gcttaataat cgtattcatt tttcttctcc tatgctttag
    21361 caaatttttc tttgtacaat tcaacaaaat cattgataag ttctcttaat agaatcgttg
    21421 tcattaaatg gtcttgccca tgaataaaaa ttacgcctac ttccatttcg ttccctgacg
    21481 cttcttcagc taaacatttc gtttgtgagt tatgcgctaa ggttaaattt tcatcagcat
    21541 ccgctaataa tcgttcaaca ttatcaaaat taccttctct cacttccttc aacactgtta
    21601 ataatgaact tctggcatct cctgcataag caacaatttc aaatcctaat aattgcaatt
    21661 ctttcctatc catttccttg ccctcctaac tatttttata ttatcgtttt tgtttcgcta
    21721 acaaatttat accaatatgc agactctttc ggatatcttt tttgtgtatc gaaatctaca
    21781 tagaatagtc cataacgctt agtgtatcca ttaacccaag agaaaacatc cattaatgac
    21841 caaatgaaat acccctttac attaactccc attttgatag catcggaaat cgcattaaaa
    21901 tgctggcgta aataatcgat tcgatcatca tctagaataa tcccatcttc aaaattatcc
    21961 ttatacccta gcccgttttc agtaatgtat attttattat aatttggata ttcgtcttgg
    22021 attcgtacaa gtaaatcata taaccctttt ggataaatga tccaatccca atcggttttc
    22081 ggaagatcct cacgcacgat tctctcgcca atccctttaa tgcaatagac ttggctacct
    22141 ttttctccgg tagtgttgta gtgaagtgtg ctttctcctt tgtactcttt aacccaatgg
    22201 ctaccatagt tattgatacc aagaaaatca tttcgatttg ctgctttttt catctctcta
    22261 aaatcattag aatcaatatc tgcagattgc ccatttgctg ctaaaatctc attaatatgt
    22321 gccattgttt catctgagta ataccctaaa taagtagcat ctaataagaa cttaattgat
    22381 aacgcatcat ctaatttcgc agcatgctta tcttcttctt tgtcacttgc tggatatttt
    22441 gtctctagcg agtgaacaac accaatttcc cctttatatc caccatcttt aaaaatgttt
    22501 actgtctttg cgtgcgcata catcatgttg tgtaaacaag ttacagtctt ctctatatca
    22561 aattgaactt ctggaggaaa cgatccgatc aaatattgat ttgaagcaac tggataaatc
    22621 tcattaaatg tactccaata atgaacctca gaaaattcat cgaaacagaa ctgtgcatag
    22681 tttacgaaat gttcaacatt ttctcgattt aaaaaatctc cattatcaaa tagttcttta
    22741 ggggtatcaa aatgatgaag agttacaaaa ggaataatac catgcttatt caattcagca
    22801 aataccttat gataatattc cactcctgtt tggttcactt ccccatagcc ttttggaaaa
    22861 atacggctcc atgcaataga catcctaata ctatcaattt taaatttttt gcataattca
    22921 gcatcaactg gatattgatt ataaaaatca ctagctggat cagcagtata gcgtcccttt
    22981 tctacaagga aagtatccca cgctacttgc ccttttccac cttcttttgt agagccttct
    23041 gcttgataag ctgccgtagc tcctcccata ataaagtctt ctggtaaagt tttataattc
    23101 attttaatct ctccttgaaa attagtttga tcaatatgcc aataatccat aaatcacctc
    23161 agcataattt taacatgtat aagtaaattt ttttacacaa cgaaaaaccg acctcgaata
    23221 tctattttat tgcgatatac aagcacttat atcccttgat gtaaaataaa tataccattc
    23281 aaaaaccgtt ttgtaaacgt ttttttgtaa aataaattta tctttttact aatttaattt
    23341 acacgaaaca agaaaacgga ctgattccca ctaaaatcaa tcccacctac taatcattct
    23401 ccaagtggct atccattatc gattcaatct tctctgttaa tggattaggc tctctgctga
    23461 tatcagagtt tcgtttcctg ctagaaaaag taatttaagt tactaaaaga gcagtttcga
    23521 tttactttaa caaagaaaag gattattata tccctaggag gatgaacaat ggttactatt
    23581 agagatattg caaaaaaatc tggttactca acggctacta tatcaagact ttttaaaggt
    23641 gatgagtcac tctctattac atctgatact aaaaacaaaa taatcgttac agccaaatct
    23701 ttaggatatg atctgacaaa aattaagact actctgtaca agattgccat tcttttttgg
    23761 atctcagagg aacagttaaa aaaggatgtc tactatcaac atgtcaaaga tgatttgaaa
    23821 aattatagta aaaaagctaa tctagatttg attttcatct ctaaaaacga tggtgtaaaa
    23881 agcattcctg acgatattac tggatttatc gcaataggac aattgacaaa aaaggaattg
    23941 tactacttaa atcaaaaagg gtataagggg gtaatccgag gaataaaccc attacctgaa
    24001 cacttcgata ctgtagaacc tgatttccac gaaatggcca gacaagcaat taactacttt
    24061 attaatgaag gtttcacaaa aattggattt attggaggaa atttctttaa cctagaaacg
    24121 ggtaaagaag agattgatag ccgtgaatat gccttccgat atcatttaga aaagagcgga
    24181 ctactcaatg aaaagtatat tttctcccaa ggatcattta ctgttgacga agggtatcaa
    24241 ttaactctta aaatgaaagc tgagttaaat gaagatatgc ccgaggcatg tttcatacca
    24301 tctgacacta tagctgccgg cgttcttcag ggctgccatg aattgggtat taacatccct
    24361 aaagatatgg cagtaataag tattaatgat gacgacatcg caaaattcgt tgcaccacca
    24421 cttacaactt ttaagataga tatcgaagag atagctaaaa cctgtgtaga tttattaagt
    24481 gatcaactgg tatattcgcg ggacatatca aagaaaacat taatcggcgc aaaattgacc
    24541 gttaggaaaa gttttatccc taaagaagag gtgtaaaata agtaattgaa acttatcttt
    24601 aaaactataa ttgtgtccac atagtagccg agcaatcggg tttatgccta ttatgttttc
    24661 cataaataat ttgatggtga tattttgacg taaatacaat atgatttttt catcgccaaa
    24721 ttgtgcgcgc taatttttgt ttatcattca gaacatttcc tcctttttat ctaagtatga
    24781 ggttggataa acgcttcatc ttagtatctt gggggatttt ttatctcgtt gggaatatat
    24841 caagcatcag agcgcgcaaa aagaactggt cgaaaaatca atctaccagt tcaatctcat
    24901 tcactgtgat actatgtttt atacagataa cgccatatta taaaacaagt tgtattccgc
    24961 aaatgggttc tattcaactc ttaaactatc tataaaagat tcagccaact ccattagcct
    25021 ctcgtaattt agttggtgaa cagcactttt ttcaaaaaat tttgaaccaa ttcctacata
    25081 agaggctcca ttgttcaaat agttttgtgc attagtctga tctacccctc ctacagccat
    25141 caaaggtaaa cttcctaaag gggcttgcac ttgattgaaa tattccggtc caagacagtt
    25201 ggcggggaac accttaacaa tatcagcacc catctcaaac attttgaata tctctgtggg
    25261 cgacatggcc ccaggaattg ttagaaggtt ctgttgcgaa gtttgaaaat caaacgcgcc
    25321 ctctctgaac tccaaatatc ttaggctgtt a
//
LOCUS       DACDCV010000034        25267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDCV010000034 DACDCV010000000
VERSION     DACDCV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.034"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..495
                     /locus_tag="IV041_001332"
     CDS             <1..495
                     /locus_tag="IV041_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8382133.1"
                     /translation="SEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRL
                     IAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQ
                     WLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDL
                     EEIG"
     gene            510..713
                     /locus_tag="IV041_001333"
     CDS             510..713
                     /locus_tag="IV041_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382134.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            729..1049
                     /locus_tag="IV041_001334"
     CDS             729..1049
                     /locus_tag="IV041_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8382135.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(1231..2013)
                     /locus_tag="IV041_001335"
     CDS             complement(1231..2013)
                     /locus_tag="IV041_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382136.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            2064..2771
                     /locus_tag="IV041_001336"
     CDS             2064..2771
                     /locus_tag="IV041_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8382137.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            2949..3290
                     /locus_tag="IV041_001337"
     CDS             2949..3290
                     /locus_tag="IV041_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382138.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            3283..3954
                     /locus_tag="IV041_001338"
     CDS             3283..3954
                     /locus_tag="IV041_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8382139.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3960..4646
                     /locus_tag="IV041_001339"
     CDS             3960..4646
                     /locus_tag="IV041_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382140.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            4653..5504
                     /locus_tag="IV041_001340"
     CDS             4653..5504
                     /locus_tag="IV041_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8382141.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            5516..5785
                     /locus_tag="IV041_001341"
     CDS             5516..5785
                     /locus_tag="IV041_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382142.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            5947..6249
                     /locus_tag="IV041_001342"
     CDS             5947..6249
                     /locus_tag="IV041_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382143.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            6246..6407
                     /locus_tag="IV041_001343"
     CDS             6246..6407
                     /locus_tag="IV041_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8382144.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            6404..6709
                     /locus_tag="IV041_001344"
     CDS             6404..6709
                     /locus_tag="IV041_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382145.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            6709..7065
                     /locus_tag="IV041_001345"
     CDS             6709..7065
                     /locus_tag="IV041_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8382146.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            7025..7270
                     /locus_tag="IV041_001346"
     CDS             7025..7270
                     /locus_tag="IV041_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382147.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7267..7686
                     /locus_tag="IV041_001347"
     CDS             7267..7686
                     /locus_tag="IV041_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8382148.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            7683..8240
                     /locus_tag="IV041_001348"
     CDS             7683..8240
                     /locus_tag="IV041_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8382149.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            8237..8533
                     /locus_tag="IV041_001349"
     CDS             8237..8533
                     /locus_tag="IV041_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382150.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            8610..9023
                     /locus_tag="IV041_001350"
     CDS             8610..9023
                     /locus_tag="IV041_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8382151.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9369..9743
                     /locus_tag="IV041_001351"
     CDS             9369..9743
                     /locus_tag="IV041_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382152.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            9973..10317
                     /locus_tag="IV041_001352"
     CDS             9973..10317
                     /locus_tag="IV041_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8382153.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10322..10603
                     /locus_tag="IV041_001353"
     CDS             10322..10603
                     /locus_tag="IV041_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382154.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10706..11020
                     /locus_tag="IV041_001354"
     CDS             10706..11020
                     /locus_tag="IV041_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8382155.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10998..12692
                     /locus_tag="IV041_001355"
     CDS             10998..12692
                     /locus_tag="IV041_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8382156.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12712..13890
                     /locus_tag="IV041_001356"
     CDS             12712..13890
                     /locus_tag="IV041_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8382157.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13853..14539
                     /locus_tag="IV041_001357"
     CDS             13853..14539
                     /locus_tag="IV041_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8382158.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14539..15699
                     /locus_tag="IV041_001358"
     CDS             14539..15699
                     /locus_tag="IV041_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8382159.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15709..16584
                     /locus_tag="IV041_001359"
     CDS             15709..16584
                     /locus_tag="IV041_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382160.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16581..16892
                     /locus_tag="IV041_001360"
     CDS             16581..16892
                     /locus_tag="IV041_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382161.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16882..17235
                     /locus_tag="IV041_001361"
     CDS             16882..17235
                     /locus_tag="IV041_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8382162.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17225..17626
                     /locus_tag="IV041_001362"
     CDS             17225..17626
                     /locus_tag="IV041_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382163.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17619..18023
                     /locus_tag="IV041_001363"
     CDS             17619..18023
                     /locus_tag="IV041_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382164.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            18035..18643
                     /locus_tag="IV041_001364"
     CDS             18035..18643
                     /locus_tag="IV041_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8382165.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18663..19025
                     /locus_tag="IV041_001365"
     CDS             18663..19025
                     /locus_tag="IV041_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382166.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            19028..19210
                     /locus_tag="IV041_001366"
     CDS             19028..19210
                     /locus_tag="IV041_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382167.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19227..22646
                     /locus_tag="IV041_001367"
     CDS             19227..22646
                     /locus_tag="IV041_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8382168.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            22697..23434
                     /locus_tag="IV041_001368"
     CDS             22697..23434
                     /locus_tag="IV041_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8382169.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            23444..>25267
                     /locus_tag="IV041_001369"
     CDS             23444..>25267
                     /locus_tag="IV041_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382170.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGML"
BASE COUNT         9167 a         3740 c         5338 g         7022 t
ORIGIN      
        1 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
       61 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
      121 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
      181 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
      241 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
      301 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
      361 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
      421 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
      481 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
      541 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgctcg
      601 ctgaaagatt cggcattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      661 ctgcagaagc aaatagaatc atagattcga ttattactat gtacgaattg taaagggagg
      721 aacagctaat gcaatatcta gaagcgaaaa tcccagttcc agaaggctat gtaattatct
      781 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      841 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      901 acccaagata tagaaatatt atcgatatcg aaaaaaacaa aaatggattt gtctattatc
      961 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
     1021 caaatttttc ggaaatctta aggaggtaag cggatgggta aatttaatag agcactagta
     1081 ttcagcgcac cactaattat ctacgcttta ggtctttggg gaagcagaca agcattaatc
     1141 ggcacaattg tatatatggt ctggatcttt atggggcttg atgaagctga atatagagcg
     1201 aaaaagccag tcgggaggga ctgacttaac ctaattaatt aaaaattgta acaaataacg
     1261 caaaaataaa gctaatgaaa ttgtattgtt aggatctact ccaatagtta tatttacaat
     1321 ttcggttact actagtccta tcaaagctgc cccagtttgc tctaaatacc acattttatc
     1381 tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt tcattagtta tatctttgtt
     1441 cttggctgta tttatttctt caaaaagctt tatagaatca tcaaggactt tcctttgctc
     1501 attaaggata tcaaaaaact tagcctcaaa ctcatcagat ggattagaag tgtctttaaa
     1561 aagtagattt tttgtactaa cttccggttc ggaagaaaat gctttttcta attcttgggt
     1621 agctacccct attctgaagt cgtacttaga catgtcaacg gcaaattttt ctgtgatttt
     1681 agagatatca gaaaaataag gttttggcat agtagctgcc atttttttag caatattaaa
     1741 acttggagat tctaattgtt tctgtattgc gaacaaagaa ggaggaatca tattagcaat
     1801 agatttgtta cgtttctcga caatttgaat gagagaatca ttgagtattg cttgcgaagc
     1861 tataaaagta tcttgaattg caattaattt tttagcttgt tctatcgcag gctgcatagc
     1921 gttaacgaca tctagagaag gagcaatggt ttcaccgatt aattttgaag aagctatcaa
     1981 tttttgataa taaggagtat ctgaaatatc caattatata caccaccttt ttacctaaat
     2041 tataccaaaa gggagagaaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg
     2101 tgactacgag cttacaagtc gcagaaacat tccgaaagga acatagagca gttttaaagg
     2161 cgattgacga tctaaaagat gggcttgcac aaaagtatgc agacctattt tgggaagatg
     2221 tgtacatcca tccgcaaaac aaacaggctt atcgaattgt ctatatgaat cgtgatggct
     2281 ttactcttgc tgtattagga ttcaacaata ctcaaaaagt tttagagttc aaattgaaat
     2341 acattgaagc gttcaacgaa atggaaaagg caattcgcaa aaatacagct tttgacacaa
     2401 ttgaagacat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     2461 tgcttaaaaa tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     2521 atacgaaagt tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     2581 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     2641 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag
     2701 aaactgcgat tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg
     2761 aaaatgaata gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga
     2821 cacaataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2881 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2941 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg
     3001 gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg
     3061 aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct
     3121 atcttatcca tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg
     3181 gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg
     3241 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg
     3301 acaacgactt ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga
     3361 aattttgatt atttatcttg ggctcacgca tgggagatta tgaaaaagaa tgatccacag
     3421 gcaacggtaa ctattaatga gtataaacac tacagagttg tttctggaac tcatcaagac
     3481 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     3541 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     3601 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     3661 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3721 ccacctagaa tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3781 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3841 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3901 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3961 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     4021 taggtgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     4081 acgatggtga tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc
     4141 aacgaaactt catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg
     4201 acttctggaa ggaattcttt tacttccgct ttgaaggtgt cacagggcgc aaaataagcc
     4261 tcaaagatga atcgaatacg actgtgagtg atgctaacgt cttagcaaat atcatcttag
     4321 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc
     4381 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag
     4441 gagctgatat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     4501 attcagagta cacatttgca gcactctgca gaatccatca cacggagaaa cacaagatag
     4561 gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac caggaaacaa
     4621 ttaagaaact tagaatagga ggataaaaaa tattggctga caacaaacga tactactatt
     4681 taaaactaaa agagaacttt ttcgatagcg acgagatggt tcttttagaa agtatgccag
     4741 atggatacat ttattctaat attcttctca aactttattt aagaagtctg aaacatgaag
     4801 gcaaactaat gtttaatgac aggattccat tcaattctac aatgcttgcg actattacaa
     4861 gacactctgt gggagttgta gaaaaagcag tacaaatatt tcgtgattta caacttattg
     4921 aggtattaga taacggagca atttatatgt ctgatataca aagttttatt gggaaatctt
     4981 caactgaagc tgataggaag agaaaataca gaaaagaaat tgaggaagca aaacagaatt
     5041 taataacaag tggacaaatg tcggacaaat gtccggacaa aactacacca gagttagaga
     5101 tagagttaga gaaagatata gagttagaga aagatataga gaatgtaacg ccttcgaaaa
     5161 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     5221 atgagcagat ggagaaactc aaaatagaat tccctaatga ctaccaagag cgaatagaac
     5281 ggctatctga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     5341 gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca
     5401 caggaagacg agagaagctt cctgagtggg caatcgacca agaagcctat ctcaagaaaa
     5461 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa
     5521 aatcgattat ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga
     5581 aatcctgaga gacgtatatg atcaactcga agaagctgga atcgaaggaa ttaagaatga
     5641 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     5701 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     5761 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     5821 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     5881 cgttccacgg cggacgtgag agcgcacttg atcaagtact caaattactg aaaggagaat
     5941 gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta ttcagaagat
     6001 tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc ttggaagagc gatgtgggca
     6061 gacaagagca acaagccaag accacgaaaa atcgcaattt ggcacggtga caaacttcta
     6121 gtgataggga cagctgaaca gttagcaagt ctcacaggct tgcacgagaa aatcgtgagg
     6181 aaaagagcaa ggtgtggcta cacagacgct aagaagagaa cgtttagata cgtggaggaa
     6241 tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     6301 acgatctgga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt
     6361 tgcacataga gaatttaaga agggaaatcc atcgattgga ggaatgagcg tggataagaa
     6421 agcagcaatg aaaagaattg ctgaattaac caagtcagaa tcttggcaag aagacaaaga
     6481 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ccaaacggag
     6541 aacgccgaga aaaattgcaa tctggcatga tgaccgaatt ttagtaacag gtactgctga
     6601 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     6661 atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt
     6721 acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa agataaaaaa
     6781 tcaaaagaac gagctgaatt agttaagtta gaaatggctg aaacaggagc aggagtgcga
     6841 agtagcaggc attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa
     6901 gggttcgatc agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa
     6961 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
     7021 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
     7081 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
     7141 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
     7201 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
     7261 cagcgaatga taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta
     7321 gccgtcttgc attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct
     7381 accgaattga aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa
     7441 gataagaatg gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc
     7501 ttcgattact tagataataa agtctgtatt gtcaaaaatt caatagatta ttccggatta
     7561 gtttgtgcca ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa
     7621 gaatacacgt acgaagtcac cggaaatata tacgagaata gcgagttatt ggaggaacag
     7681 cgatgaataa acaggaagca atcaaaaagt tagaaagtat taaagcgata ggaaatgatg
     7741 caatagctgc ttgctataac gagagtataa attcaggtat tacgttaatg aaaaaaatag
     7801 acgaaccgca gaaaccagtt attccacaac tcgtggccgg ttggcttgag aaatctacgg
     7861 acccttttac aaaagctgaa aaaatagcgt atttaatcaa atctaaagat ggtgattcat
     7921 attatttctg tgattggttt gtacgagatg gcatagtgac gcaagagcaa ggagaagaat
     7981 tactcgcttg ggcaacaaga caatcatatg aaacactatt gagcctatac aacggctacg
     8041 aggttgagaa agagccgtta tgggcaataa agaatgccga tggaaactat cttactaaat
     8101 gtgctttatg gggaaaagat ggagtaaatt atagttttga atgcaatcca tctcatcgat
     8161 tgcttttcac tgataaagca acagcggatg ctgcagcatt gttggtgaat ggaacagtgg
     8221 aagaggtggt agaaagatga aactaaaaga cggattttac gctagcagtc acggtatcgg
     8281 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgcaagaaat caaaattcaa
     8341 agtcggtgac atggtccgct gtgaagcaga agagttcgtt tatccgttca gaggatacgt
     8401 taaaaagata ctgtcaaact cagcaatcat tcgtattgaa aatacaatga agtgtgatcg
     8461 ttcgactgca aaacacaagc attatttagc tgtagcgaga ttagttgaca tggaagtaat
     8521 caagagcaaa taaaaaaagc cggatcgctc cgactgattc aataaatcca acacatttat
     8581 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
     8641 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag
     8701 gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccaaagc
     8761 acggcaataa ggcagaagac gcgatcattc agatgatgga tatagaagcg gagagagatg
     8821 cgattctagc ggctttgatg gctcttagtc taattagtcg ccagatactc tactacagct
     8881 tctgtgtgcc agatagcttc tcaaactaca ggattagccg tgaagtgggt tattcagaaa
     8941 gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca tataaacacg
     9001 gaagaataat tgcttataaa taatttggcg gttttttggc ggaatgatgg cggtttttag
     9061 ctatttacca gtgatattat ggtagtgtcg aaagattagt gataggtcta agacaaaata
     9121 aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc ttcgatagac agcagcaaat
     9181 aaactaaagg atgtgggggt cagctcctac agttagttca tgtgttgctg tctattaatt
     9241 gcaactcttt cggttttatt gagtatttac tcaacttaaa aaaactgttg tttgtagatg
     9301 taagctgttc ctatttgtac atagtaaaaa atgatataat aaaaatatta cgaaaggggt
     9361 gagagtgcat ggaagacaaa atgtatttaa gacaaaaagt tttcacaaaa aaagatattg
     9421 aaaatcaaat taatggattt atacagaaat tagaaaatgc aaattcttat ttgataaata
     9481 aggaactaaa taaagcttat gaccagtgga aagaagtata tgatgagttg aaattgattc
     9541 aaaatgaaac aaaattagta cgaacggaga aaaagaatga aaatagtttc ttttttgatg
     9601 gatatgctat attgatgttg gaaactgtag caaaacaaaa tataaaagct cccaaaaaag
     9661 aactatctga caatattgat aacgcattag ccgagttaag gtattacgtt atgcaaataa
     9721 aagatgttag gatcactcgt tgagtggtct ttttattttg cacaaaggag gtaacaacaa
     9781 tgtatagacc acaatactta gaacagaagt atgaagtaat cactgtgcaa aatggtaacg
     9841 gagagatagt acgaaagtat agaagaccaa taaagagcga tacatataaa cgaaaggaaa
     9901 gcaatgaagt tattccattg tatggcaaaa gaatagctaa gcattaaata agattgtgaa
     9961 aggagacggg acatgaacga ggaattctat agatggctat tacagttgat aagagaagat
    10021 cgtttggtta agttctatca gtctcctaaa tggcgcaggc ttagagagaa agcgatgaaa
    10081 cgagatcact atgaatgcca agagtgtaga agactaggta agtatcatag agtagagaac
    10141 gttcatcata taaaggaagt caaggataga cctgacttag ctttagattt agataatctt
    10201 atttgtttat gtgttgaaca tcataatgaa gttcatggca gatatcttac agcattggat
    10261 aaacaagaga agaagataga aagctttgct aacttcgatg caagtgaaag gtggtaagtg
    10321 catgatcatc aatgataatg gcagagagta tgatacagaa aagattgaag agtattcatc
    10381 ttatactcag ggattaatta aacgtttgat atacgttcgc tatgtaggta ttagggatct
    10441 gttatcagat aactgttgta gtaaatacaa agtgaatcaa gtaagagaag cgttgaataa
    10501 agataataac gtcgaaagaa taaaaaatgt ttttggatat agtattgaag agattaatta
    10561 ttacattgac ttcgctgaag ctttcattcc gatggtgaga taaccccccc ttaaaataaa
    10621 tcgcaaattt tttgggggtg atgaaacgga gggggctgtc aggaaaagag attttttcga
    10681 actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa ttgtctcaaa atcgctacag
    10741 agaaaaatta cgccgctctt taataagcca attggaaagt cagaaaacaa atattgagcc
    10801 attcttagat aatgttgatc gttatatcag tttatgggaa acggcgatat cactggaaga
    10861 agatatatcc gagaacggca ttagactgga gaatggtaaa aagaatgaat cagtagcgtt
    10921 gcttgtttct gtcaacaaac aaatgggatt gatgttggat aaacttgcca ttactcctga
    10981 attggtaggt gaagcaaatg aatcaattcc tgagttataa gcatattgaa aattggttca
    11041 aagctataga agaaggcact atcaaggtat gcaaagagca attattgcta aaaaattatc
    11101 tagaagaaag agtctttact agagaagata tttacttcga taagcagatg gtagaggatt
    11161 caatcaatat accagcacaa tactttccat tcgaattaat tccgtgggaa aaatttctac
    11221 aatgttttat ttatggtgtt cgatggaaaa aagataaaac actagtgttc aatagatatc
    11281 tttcattaat gggacgtggt aatggtaaaa ctggttttgc ttcttggaac aacttctttc
    11341 tactaaccgc taaacacggt attaaaaatt atgatattga tatctatgcc aataatgaaa
    11401 gccaagcaaa gactagtttt gatgatgtat ttaaagtaat taaagatcat cctgatttag
    11461 ataaaaaagt atttaaagct acgaaggaag ttattcaaaa tatcgctaca aacagcaaac
    11521 ttcgttataa cacggcaaat gctagaacaa aagatgggaa gcgaccaggt gcaaaccgct
    11581 ttgatgaaat tcacgaaaat gaagattatt caatgataaa tgtggctact tctggtggtg
    11641 gtaaaattcg agattataga gaattttatg atacaactaa tggtcatgtt cgtggtggtc
    11701 cgcttgatga cattatagaa gaatcaaaaa tgattctttc tggagaactt ggaattgaca
    11761 aggatggagc agaattttct agtttgtttc catttatttg tcgcttggat aacgataatg
    11821 aagttgatga tcccgacatg tgggaaaaag cttgtccaac tattaattac aatgcaaatt
    11881 taaaacggaa aatgtttcaa gaatactctc aaatgcaacg taatgctggt ttaagactta
    11941 cgttcatgac caaacgaatg aacagaccta tggaagatac acgatttgct gttgcttcat
    12001 atgatgatgt tctgcatacg aaagaaaaag aatttcctga aaaaatggat gaagtgatag
    12061 gaacagtcga ttttgctgat agacgagatt ttgccagcgt tgggttgcta ggaaaatacg
    12121 ataaagatgt ttattttaca caacatactt ttatccacga atcagccctt cgattacaaa
    12181 acatcaaacg agaggttata gatatttcta tagatcaagg aaaatcacag atcgttcatg
    12241 gaaaaaatat agaagctgat tatattgtag gttggtttct tgaaatgagt aataaatatt
    12301 atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat attgaagccc gctttagaag
    12361 aagcaggttt tactgtggaa attgttcgaa gcggatctgt tacacatggt atgttaaaag
    12421 atctggttga tgaccttttt attaatcaac gtttattttt tggtgacgat gcgattatgc
    12481 gttggtattg catgaatgta tatgaagagc atatttctaa tggaaatata cgctatgaaa
    12541 aaatagaacc tgaaactaga aaaacggatg gctttttttc attccttcat ggtttgaatt
    12601 ttttagatga tatttatgat tctgctcctg taacagtcac aaatagctca gtagaaaata
    12661 caggaactgg atttactcct ctagtattct aacttgaaag gaggtgagaa agtggggatt
    12721 tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg ttcctttaga agaatacttt
    12781 tgtaaattgc aagttgattt tgtgtatcga aaatttgcga ttgaaacttg tattgatttg
    12841 attgcaaatg cgatgagtaa agcggaattc aagtcatatg aagatggaaa aaataaaaag
    12901 aatgatcttt actataggct gaatgtagct cctaataaga aaaataatgc aacagaattt
    12961 agaaaaaaac tgatcaggag attaatattc tacaatgaag tattgatcgt ttctccgtct
    13021 aataattcta gcgaaatatt tattgcggat agttgggatg tcacagaata tgcattgaaa
    13081 gatgatgtgt tttctcaagt gcaaattaac aacatagtcc ttgatagaga atttctagaa
    13141 agtgatgtta tctatataaa atacgcagat caacaaatta ggcaactagt cgatgcgtat
    13201 tatcaagcgt atgggaaact catttctagt gccatgaatg tttacaagcg ctctaacgct
    13261 cgtagatacg tactgaaagg gaatttattc cgaccgcaag acaatacaac acaagatcaa
    13321 atcaataaaa tgatgacatc acaatttaag gcttttatgg aagctgataa tgcaggtgcg
    13381 gtatttcaat tacaaaatga gtacacatta gaagatttca gcggaaactt ccaaagcaat
    13441 tcaagagata taaaaaactt aatagacgac atctttgaga tgacagcagc agcgtttcac
    13501 gttccgaaaa acctactaaa gggagacatg agtgggttat cggatcaagt ggacgctttt
    13561 ttaatgttcg aaatcatacc gattgctgaa cttattcagg atgcgtttaa cgctagtctc
    13621 tatgaagtag aagaatactt gtcagggaat tttgtacgtg tggatacaac tatgatcaag
    13681 attactagct tcaaagattt ggttgacgct attgatgtag gcattagaaa tggagtattt
    13741 acaatcaacg aaggaagaga acgcgttgga aatgatcgct ctgataaggc gatggcagat
    13801 gaaatattta taactaaaaa caatcaacaa gtatcgaaag gaggtgaggc gaatgacgac
    13861 aatgaaaaca tttctagcag taaagaatga aggcgcagta ccgcaaattt ttattcaggg
    13921 atttattggt tctagttggt tctttgaagg gaatactgac aagggaatca aaaatatttt
    13981 ggatagtcta ggtgatcaag aagaaattga agtagtaatt aattcaaacg gtggagacgt
    14041 atttcaaggg attgctattg ggaacttact taagtcaaat aaagcaaaag ttaacgttgt
    14101 gattaacggc ttagccgcta gtgctgcttc aattatcgca atggctggcg atactataaa
    14161 aatctacaac aatgcacaat tgatgattca ccgcgcttcc acatacggag aaggaaatgt
    14221 cgatgacttc cgtacgattg ctgaccaact ggaatcaatt gataaatcgg taaaggcttc
    14281 atataaaaca cgattcaatg gcacagatga agcattgcaa gaacttcttg aaaaagaatc
    14341 gtttatggat gcagaaacag ctttgagtta tggattggtc gatgaaatta tcgatgcaga
    14401 aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt gaagaaattt tgaatgacgt
    14461 taaagaaaaa agagcagaaa aaattgctgc atttacagca gcattaaata aaacatttgg
    14521 acaaggagat gtaaaataat gacagttaaa aatttaaaag gtgtaacagc tgcaagcgac
    14581 caattgatga aagcttttaa agatggtaac gaagaatctt ttagcgcagc tatggtaagc
    14641 ttatctaagg aaattcagga taaaatttta gaagaagcaa cagcaaaaaa tcaagatcaa
    14701 ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac aagaaacaaa attctataac
    14761 gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat tagtgccagc tactgtattt
    14821 gaacgtgtat ttgaagattt agaacaatct catccactat tgcaaaaaat tacttttgtt
    14881 aacacaactg gtgtaacaga atggattgtg tcacgtggag tcaatccagc atggtggggt
    14941 aaactttgcg aagctgttaa aaaagtttta gataatggct ttgatgtaat taacatgaag
    15001 cagttcaagc tatcaggtta tattcctgta tgtaaggcaa tgcttgattt aggtccagta
    15061 tggttagatc gttatgtccg tactgtttta gtagaatcat tgagaattgc attagaacaa
    15121 gcaattgttg atggtactgg taaagatatg ccagtcggaa tgatgcgtga catgagcaaa
    15181 caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta ttacagcttt agatgctgta
    15241 actatgggcc gtttgatggc gcgactatca aaattcaata tcgaaggtgt gaatgatccg
    15301 atttatcgta atgtgaatcc ttctgatgtg gtcctaattg tgaatccaac tgattactgg
    15361 tctaaagtat tcccagctaa gactgtacta actgctaatg gagaatacgt acaagtattg
    15421 ccagtaccag tttcagattt gcagtcaacg gctgtgccag aaggaaaagc agttattggg
    15481 gtagcctcag attacttcat gggtgtagga tctacactaa aaattgaagc ttcagatgaa
    15541 taccattttg ttgaagacga acgcatttat ctagctaaac aatatgcaaa cggtcaacct
    15601 aaacgtaacg atagtttcat tgtattagat attagcgctt tgggaactac tactacaact
    15661 acaaaaccaa caaccacaac aactacaaca caagcgtagg tgatcagaat gaagtatatt
    15721 ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg aagtttgttt aactaatctg
    15781 aagaaactag gaatcaaaga tatcgtattg cttttcagca gacacgatga tcagattcct
    15841 attttttttg agaaggaata tggtgttgaa gttcatgtgt acgatgatct gcgggacgac
    15901 aaagagtata ttccttcgat taaaccatat ttatggtgga aatatttaga agaagatcat
    15961 tcgcgtgagg acgaccgata tttctatatc gattcggatg tcattttcaa taaaagaatt
    16021 aatttgcgca aattgccttc taaagatgat gtttggtatt gtagcgactg ctgtagttat
    16081 ctaagtcttg attatattag aagctgtgaa aacggagaaa atattctaaa agatatggca
    16141 aacattgtaa atgttacagt agaatctttg gaaactataa acactaattc aggaggcgca
    16201 cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa aggtttatct ggattctaat
    16261 cggctatatc gctaccttag agggcaaaaa acaaatatac aaatctggac agccgagatg
    16321 tgggcacggc tttggaacat gatgtatttc aatattggtc ctaaagttca cgaggaatta
    16381 gacttttgtt ttgctactga tccaatagaa aaagttaaag aagtaaaaat cttacacaat
    16441 gctggagtaa caacaaacga tgaagattta tttttcaaag ggagatacgt gacttctacg
    16501 ccttttgatg aagatttatc atttgtaaac aagaaaaaat gctcttacgc atatgttaaa
    16561 gcaattaagg cggtggttag atgacgcctg aacaagtgac tgaagaattg ctaatagctg
    16621 tgaaggataa tatttacgtt acctggaacg aagaagatga gtcaattaaa aagatgatag
    16681 ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac actttctttt tctcctgaaa
    16741 gcttagaata cggattgcta atcgaaagat gtagatacga ctggaatcgt gctttagatg
    16801 agtttgaaca aaatttcgct agtgagttat taggtttcat tcaacattat gcgctacaag
    16861 aatatattgc aggtgatgtg aatggcgaat aatcgtagac tcgaagaaac attcaacgat
    16921 ggttggttaa agattttgac gcaaactacc aaaagaaatg aactaggaaa aaagattggt
    16981 gtagaagata cagaaatcac ttctttaaaa tttagaaatc tttccatgag agatagtgat
    17041 ataacagcta tggatgcgat gggatcgaaa ttaactaaga aagtaaagac tccatttcat
    17101 ccaatcgcca agaaatttaa taaagatcaa tattttatcg taatcgatag tatgcgttac
    17161 aacgttatct atgccgatta cgataatttt tatatctatt tttatcttga aagtgtgggt
    17221 gaatatggtg attgataatt ctaaagaaaa agaacgttta aataagcaaa tttctgctat
    17281 caaaacttcc ttagaagagc attttggcct caaactcttt caagactccg taggcgagga
    17341 tgagctacct gatgatttta attacttcat tctcgaaaca ggagaaatag aaatgatcac
    17401 tgagccaaaa tatagcgtgg gtcaaaatct atatctaact ttctattcag aaaatagaga
    17461 agatttaaca ggagactcac tagatattat ttcattgatt caaaatcgtt cgattcgttt
    17521 tcagagaatg gatcccaatc atttaaaact agagaaccaa gatcgctata tcgatcaatt
    17581 ggtatttacg tttagacgat tattgaagag tgattgtcat ggctaaaaat agttgggagc
    17641 taaaaataaa tggacatgat gaacttcttg tgcggatgga acgctattca agcgagagcg
    17701 aacgactgat taacgaagca ttgaaatcaa aaggttcggc tattgcagtg gataggatta
    17761 cagaaaaaat tcctgtttct gaagcagatt taagaagagg gcaccaacac gcaaaaaata
    17821 gtcgtccact taagactcaa tacattaatt tgggtttcat cattagacct acaagaaaat
    17881 ttgagtattt aaaatatcct gatttgggga taggtacttc taaaagaaat cagccggacg
    17941 aatttatgag aagaggatta ggtcttgcac ttgatccaat tacagaactt ctgattcgtc
    18001 aattcgataa attaaataaa tagggggaac aacaatggct aaaacaaaaa ctgtagtaac
    18061 aacgttcgat aacgtaagta tcaaacgaat tgcttttaat tttaagaacg caggaaatgc
    18121 aatcgcaacg aattgtaatg gacaattaga tggcgaaaca gaaatgcaaa cggtggttaa
    18181 aaaatgtgga gcgacagaag taaaatcaaa atctaaacca atcaatatga cggtaacaat
    18241 tactgcacat gtaccgatgg aagtttatcg acgtttcaat gggttgaaac aagatggacg
    18301 tattaaacca ggcatttact cttacggtcc tgattccgta ggcgaagatt tctcacttgc
    18361 tgcagagatc gtggatgact tcgaagaaaa tagaaagtta gttggtatgt tagcatgcac
    18421 ttcgaataca ggattaacat tctctattga aaatggtgcg gatgaagtag ctgcgttaga
    18481 actagaaaca aaagttatgc aagatgaatt tggtaaattt tatcatgaag caattgttgc
    18541 agaacttgaa gaagacttaa cagatcaatg gatgacgaat ctatctgctg atgtgattaa
    18601 aaaaacttca gttgtgacaa ctacggccac tcaatcacag taaaaaaaac ggaggtagcg
    18661 aaatgaacga agattactca aaaattgaac taaacgatgg aacaattttg aatttagaac
    18721 ctaaactgaa tatcaagaaa ttattgatga tcaatagaga ttttaacaca gacgagtttg
    18781 caaaaatgac tgtgggaaaa ggatccatgg atatttctgt tattcaaggt gcaaaggctg
    18841 tgtatattgc ttaccgccaa gcgaacatga ctgattatat ttcattcgat gaatttatcg
    18901 ataaatggga ttttgatatg gctactgcca gctatattta tcaattgatg atgttcaaac
    18961 aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa taaggaaaaa aagcttcaaa
    19021 agtaaaaatg ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga
    19081 cgttttttct atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca
    19141 aatggcttac aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatctca
    19201 gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt
    19261 tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta
    19321 aaaatagagc agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag
    19381 acaagttagg aagtaaatta tcttctttag aaaagcagta tgaattacaa agccaaaaag
    19441 ttgaagtaac tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg
    19501 aagttcagaa acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa
    19561 acgaaattga taaaacgagt aatacactag ctcaagcaaa aggcgaaata cagacgtacg
    19621 agtctacaat gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga
    19681 ttgaatccga atacaaaaaa tggcaagtaa ctgctggtca atcagcttct gaatccgaga
    19741 aattagcgaa agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag
    19801 atatcctgag acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag
    19861 caatgcagat ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag
    19921 ctgctaaaaa tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata
    19981 atttaatgga agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga
    20041 aacaagcagt ggactctgct aacagtgtag gtagttccca gagtaaaata caagctaatt
    20101 ttggtttgac taaacaagag gctgaagaat taacgaatgt agccagagac atttattata
    20161 aaggttttgg agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt
    20221 taggcgattt aaataatcaa gatttacaaa atattacgga acaagctatg gtcctagaaa
    20281 acaccatggg cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaact
    20341 tcggattgag cgctcaagat gcaatggatt taatggtttc gggtactcaa aacggtttag
    20401 ataaaacgca cgaattaggc gacaatatgg cagaatatag ccaattatgg agtcaaatgg
    20461 gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg tttagatgcg ggtgcttata
    20521 accttgataa agtcaatgac ttagttaagg aaatgggaat atcgttaaca gatggtcgat
    20581 ttgagcaaaa catggatatg tttagtgaaa gtactagaaa agcttttgaa gagtggaaaa
    20641 atggtggagg aacacaaaaa gacgttatta attccatgat tcaagatttt agcaatatgg
    20701 atggtcaata cgaccaatta aataaagctt ctacaatttg gtctgcactt ggcgaagata
    20761 acgcgatgaa agttgtccaa tctttgactg atgttaacca tacatttgat gatgttagtg
    20821 gatctgcaca aaaaatgaat gaagattcta ctactccgtt acaagaatta aacggaaaaa
    20881 tagctgaatt aaaggattca ttagctccta taggcaacac aatcatagat gcactcgaac
    20941 cagtaattga ttttctagga aagatggctg atgcgtttaa taatcttcca caaccagtac
    21001 aggattatgc cgtagcgatt ggcggattga ctgctgcatt tactttatta atgccaataa
    21061 tagttggctt catggctcta ggtggtccta ctacattaat aataggagca gttattactg
    21121 ctattgctgg agttatagca attataaaaa actggggcgc aattactgac tggtttaagg
    21181 gaatatggag taaattcact gattggttgg gtggtacttg ggaaagcata aaagaaggtg
    21241 cctcatcagt ttgggatgga gtcaaagaaa cctggtctgg atttgtagat tgggttcaag
    21301 aaatttggca aggagtttct gattggtttg gagagttatg gagcggatta gttgaaggag
    21361 cttccaacat ctggcaagga gtccaagaga cttggcaagc attcgttgat tgggtttcaa
    21421 atatttggaa cggagtcaaa gaagtatggt cgattatttg ggcagacatt gtaggaattg
    21481 ttcaaatacc atggacatta ataacgtcat tgattcaagc tggtattaat attatcgtgg
    21541 gtatctttga tgtagctgga cagttattag gcgcagcttg gcaagctgtt tggacaccta
    21601 tttctgattt ccttaaaaat acttgggata ctatgacaca atggataagc atcgcttgga
    21661 atggaattgt aactacattc catactatat ttgatccagt agtggcatgg tggaatggta
    21721 tatggacatc tattagtact acggcttcaa atatttggaa ctcaattagt gcaacagctt
    21781 ctagtatttg gaatagtatc aagaatacaa tcactagctt ggtacaagca gctgctacag
    21841 taattcaaaa tatttggtca actgtatcta gttggttagg tggaatttgg aattcaatca
    21901 gctctacagc atcaaatatc tggagtagcg tgactagtag tataagcaat gctataaacg
    21961 cagctaaaag tgccattcaa agtgtttgga atagtatatc ttcgtggatc agcggaattt
    22021 ggaacggtat caaaaacact gctttgaatc tttggaatgg aattacaagc actattagct
    22081 ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta acaggtattg
    22141 tttccgacat attcaataat gttaaaagca cgatagctaa tatatgggaa ggtatcaaaa
    22201 agactgttag cgctccgatt gattggatta gagataaaat cagtagtgtc tttgataatt
    22261 tgaatatttc tataccacat attccgttac cacattttaa attgagcgga gaattcaatc
    22321 cattgaaggg gaaaatccca acattgggtg ttgattggta tgcgaaaggt agtgtgttta
    22381 attctccaaa tattatcggt gtaggtgaat caggacctga agcagtttta cctttgaaaa
    22441 gatctgtgct gcaagaaatt ggtgatcgta tcttgagtag cacctcagtt tcatctagga
    22501 cacaagcggt tcaacctgtg aacaattacg aattcaattt cacaattgat ggtaacgcag
    22561 atgaagtcac tatgaagcaa acaactcaac aaatcattga tagcattaca aaagttcaaa
    22621 atgataatgc ttcggcatgg cgttaaacag gagagtattt ctcctgtttt tttagtatta
    22681 aaaaggatgt gaaaaaatga ctgattgtat acattctata atcgatggat ttcctgatta
    22741 tttgcataaa ttggctttag ctgaaagacc aaccatacct tctccaaaaa gacagagagt
    22801 tgaaacttct gttttaggaa ggttaggtgg cttagtgcaa gattactcgt ttgaagacat
    22861 gtcgtttaca ttgcactata actatttaga ggatgtggaa gaccatcaag cgttcaagca
    22921 atcgttttat atcatgcgtc attggttaaa ctatgcaaag aaattagaat tctctgacga
    22981 tcccaacgtc tattacgtta tccagactat cgatattggg gatgcagaaa acgatattgt
    23041 tgaatgggga gagttcgatg taaatattac tgcgaaacca ttcgcaagag ttcaagaaga
    23101 tgtacctata accgtagata aaccacagtc atttaacttg ctgaataata gtttagaaga
    23161 aagttttcca aagattatca tcactccttc agctacttca tgccagttca tcttaaatga
    23221 ttatgtgttt agtttcgaag gcttagtagc aggaactgat gtagtcattg atagtgattt
    23281 gatgctttgc tacgaagagc aatcggacgg agatatttta gatcggtcca acaaaatgaa
    23341 gaccatgcaa tatccgacat tgcaagtgga tatcaatcat tttaattgca ctggtttgag
    23401 caaaatacaa atttatcgta acgggttaag gtaggtgaaa tagatgatcg ataatttaat
    23461 aactatttac gataaaaatg acgcgaataa tttagctgaa catttatatg atacgcaagg
    23521 tttaggcgct ttgtcagact ggttaacagc tactgttagc aataaactaa acggagccga
    23581 gatatttcag ggtacttatc caataagcgg aactaatgca gatttgattg tagaaggacg
    23641 tattattcag tgttatgtag atgaaaatcg agcaaaacag cgtctacgga tttattatgc
    23701 aaaaacttct gtaataggca atacgataga agtaaaagct gaacctattt tcaatgatat
    23761 aagaaaatcg gtgttgaata aatatgacag cggaacagaa aaaatcactg ctactcaggc
    23821 atggcaaaac gcaaaagctt tagcgaaacc agctatccct tcgcagtttt ctttctcgtc
    23881 attagtagat acgcttgcta atgtgaagat agaaaaagcg aattttttag aattctttgg
    23941 tggaaaagag ggatctattc tagatcgatt tcatggggaa tttcttaaag ataataacac
    24001 attacatcat gaaaaaaggc taggtacgga tcataaaatc aaagcgattt atactaaaaa
    24061 tttaactggt cttgacttag agatagatgc tcaaagcgtt ttagttggag tttatccatt
    24121 cattagcagt tcttcagaag gagaagacga gatcactcta ccagaagaag tcattttcac
    24181 ggattacgtg gatgattatc ctgctggata tgtttctttt gttgatttta aagacaaagc
    24241 gactgatgta gccacattaa gggaagctgc taaagactgg ttgaaaacaa acatagataa
    24301 acaaaaacca caagtgagtg gttcgattga attagtacca ttgaggcatc aaagaggcta
    24361 tgaaaaattt gttgatctag aaaaagtttc gatgggtgac ggagtagatg tgtatcatcc
    24421 acagttaaaa gtgaatatgt cagcgagaat tgtggaatat acgtttaatg ttctaaccaa
    24481 ttcatacgat aaattagttg taggaaacgt caaaacaaac ttcttagaaa acacagagaa
    24541 taatgtaagc aatttgatta atgatgccat tgatcaattg aaaaatggtg gcgaaatcag
    24601 tgatttaatc aatgatattg tagatcatca aactgatatg attactggtc aaaatggtgg
    24661 ttatgtttta ttagatccta aagaagcgcc tagtcgtatt ttgattatgg acacaccaga
    24721 taagaatacc gcaaggaatg ttttacaaat caacaatgct ggtattggtt tttctaaaac
    24781 tggcattaat ggaacatatg aaaccgcatg gacattagat ggcggattca atgcctcgtt
    24841 tattacggct ggtgaaatag taggaattac tattagaggt actacattaa ttagtgatgg
    24901 cactgattat agaacaagta ttgctaatgg caaaatgact tggtactcaa aaaaagttaa
    24961 caaagatatt atggagctag aagcacgtga ttatgtaagt gctgatgccg gtattgtatc
    25021 atacaccatg aaaactggtg gtggtttcat gattagaaat ccacagggta acttggtttt
    25081 tagtacgtgg gataatggta ataacagacc gtttctatct tttggtgcgc ccaatttcag
    25141 gtatagcaat gctagttatg taacttctgg cgacggtagt tctttaagca ttaatggtag
    25201 tgcgggtaac tcatgggaat ttaaggtagc tggtaggact atgaaattta ctagtgatgg
    25261 tatgcta
//
LOCUS       DACDCV010000035        24606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDCV010000035 DACDCV010000000
VERSION     DACDCV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.035"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV041_001370"
     tRNA            8..81
                     /locus_tag="IV041_001370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV041_001371"
     tRNA            101..173
                     /locus_tag="IV041_001371"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV041_001372"
     tRNA            201..274
                     /locus_tag="IV041_001372"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV041_001373"
     tRNA            288..361
                     /locus_tag="IV041_001373"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV041_001374"
     tRNA            385..474
                     /locus_tag="IV041_001374"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV041_001375"
     tRNA            487..560
                     /locus_tag="IV041_001375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV041_001376"
     tRNA            579..654
                     /locus_tag="IV041_001376"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV041_001377"
     tRNA            656..726
                     /locus_tag="IV041_001377"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV041_001378"
     tRNA            740..813
                     /locus_tag="IV041_001378"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV041_001379"
     tRNA            838..925
                     /locus_tag="IV041_001379"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV041_001380"
     tRNA            947..1018
                     /locus_tag="IV041_001380"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV041_001381"
     tRNA            1041..1130
                     /locus_tag="IV041_001381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV041_001382"
     tRNA            1143..1216
                     /locus_tag="IV041_001382"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV041_001383"
     tRNA            1235..1307
                     /locus_tag="IV041_001383"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV041_001384"
     tRNA            1311..1383
                     /locus_tag="IV041_001384"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV041_001385"
     tRNA            1388..1458
                     /locus_tag="IV041_001385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV041_001386"
     tRNA            1471..1544
                     /locus_tag="IV041_001386"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV041_001387"
     tRNA            1556..1627
                     /locus_tag="IV041_001387"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV041_001388"
     CDS             1893..3086
                     /locus_tag="IV041_001388"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8382171.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV041_001389"
     CDS             3216..4688
                     /locus_tag="IV041_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8382172.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV041_001390"
     CDS             4771..5559
                     /locus_tag="IV041_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8382173.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV041_001391"
     CDS             complement(5626..6294)
                     /locus_tag="IV041_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8382174.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV041_001392"
     CDS             6409..8121
                     /locus_tag="IV041_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382175.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IV041_001393"
     CDS             8124..9893
                     /locus_tag="IV041_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382176.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IV041_001394"
     CDS             complement(10098..10664)
                     /locus_tag="IV041_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8382177.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IV041_001395"
     CDS             complement(10664..11620)
                     /locus_tag="IV041_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8382178.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IV041_001396"
     CDS             complement(11620..12276)
                     /locus_tag="IV041_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8382179.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IV041_001397"
     CDS             12646..14664
                     /locus_tag="IV041_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8382180.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IV041_001398"
     CDS             15091..17505
                     /locus_tag="IV041_001398"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8382181.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IV041_001399"
     CDS             17853..19406
                     /locus_tag="IV041_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8382182.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IV041_001400"
     CDS             complement(19787..21571)
                     /locus_tag="IV041_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382183.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IV041_001401"
     CDS             complement(21586..23307)
                     /locus_tag="IV041_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382184.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IV041_001402"
     CDS             23407..24168
                     /locus_tag="IV041_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8382185.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7669 a         4488 c         5190 g         7259 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tcccta
//
LOCUS       DACDCV010000036        23076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDCV010000036 DACDCV010000000
VERSION     DACDCV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.036"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IV041_001403"
     CDS             102..2543
                     /locus_tag="IV041_001403"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8382186.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="IV041_001404"
     CDS             complement(2873..3577)
                     /locus_tag="IV041_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382187.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="IV041_001405"
     CDS             complement(3764..4009)
                     /locus_tag="IV041_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382188.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="IV041_001406"
     CDS             complement(4026..4877)
                     /locus_tag="IV041_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8382189.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="IV041_001407"
     CDS             5043..6047
                     /locus_tag="IV041_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8382190.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="IV041_001408"
     CDS             complement(6288..6644)
                     /locus_tag="IV041_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8382191.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="IV041_001409"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="IV041_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8382192.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IV041_001410"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IV041_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8382193.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11723)
                     /locus_tag="IV041_001411"
     CDS             complement(11004..11723)
                     /locus_tag="IV041_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8382194.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="IV041_001412"
     CDS             12043..12564
                     /locus_tag="IV041_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8382195.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="IV041_001413"
     CDS             12623..13483
                     /locus_tag="IV041_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8382196.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13590..15983
                     /locus_tag="IV041_001414"
     CDS             13590..15983
                     /locus_tag="IV041_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8382197.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="IV041_001415"
     CDS             16165..18006
                     /locus_tag="IV041_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8382198.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="IV041_001416"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="IV041_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8382199.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="IV041_001417"
     CDS             19359..21458
                     /locus_tag="IV041_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8382200.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="IV041_001418"
     CDS             21654..22517
                     /locus_tag="IV041_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8382201.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23076
                     /locus_tag="IV041_001419"
     CDS             22622..>23076
                     /locus_tag="IV041_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8382202.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaa
//
LOCUS       DACDCV010000037        22748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDCV010000037 DACDCV010000000
VERSION     DACDCV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.037"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..443
                     /locus_tag="IV041_001420"
     CDS             33..443
                     /locus_tag="IV041_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8382203.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            436..1137
                     /locus_tag="IV041_001421"
     CDS             436..1137
                     /locus_tag="IV041_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8382204.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1137..2723
                     /locus_tag="IV041_001422"
     CDS             1137..2723
                     /locus_tag="IV041_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8382205.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2742..2963
                     /locus_tag="IV041_001423"
     CDS             2742..2963
                     /locus_tag="IV041_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8382206.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2960..3721
                     /locus_tag="IV041_001424"
     CDS             2960..3721
                     /locus_tag="IV041_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8382207.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4101..4610
                     /locus_tag="IV041_001425"
     CDS             4101..4610
                     /locus_tag="IV041_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8382208.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4685..5224)
                     /locus_tag="IV041_001426"
     CDS             complement(4685..5224)
                     /locus_tag="IV041_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8382209.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5364..5975)
                     /gene="rpsD"
                     /locus_tag="IV041_001427"
     CDS             complement(5364..5975)
                     /gene="rpsD"
                     /locus_tag="IV041_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8382210.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6291..7088
                     /locus_tag="IV041_001428"
     CDS             6291..7088
                     /locus_tag="IV041_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8382211.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7116..7751
                     /locus_tag="IV041_001429"
     CDS             7116..7751
                     /locus_tag="IV041_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8382212.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7771..8544
                     /locus_tag="IV041_001430"
     CDS             7771..8544
                     /locus_tag="IV041_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8382213.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8952..9749
                     /locus_tag="IV041_001431"
     CDS             8952..9749
                     /locus_tag="IV041_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8382214.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9727..10140
                     /locus_tag="IV041_001432"
     CDS             9727..10140
                     /locus_tag="IV041_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382215.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10124..12769
                     /gene="mutS"
                     /locus_tag="IV041_001433"
     CDS             10124..12769
                     /gene="mutS"
                     /locus_tag="IV041_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8382216.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12787..14895
                     /gene="mutL"
                     /locus_tag="IV041_001434"
     CDS             12787..14895
                     /gene="mutL"
                     /locus_tag="IV041_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8382217.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14917..15477
                     /locus_tag="IV041_001435"
     CDS             14917..15477
                     /locus_tag="IV041_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8382218.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15562..17550)
                     /gene="treP"
                     /locus_tag="IV041_001436"
     CDS             complement(15562..17550)
                     /gene="treP"
                     /locus_tag="IV041_001436"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8382219.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17773..18486
                     /gene="treR"
                     /locus_tag="IV041_001437"
     CDS             17773..18486
                     /gene="treR"
                     /locus_tag="IV041_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8382220.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18563..19009
                     /gene="msrB"
                     /locus_tag="IV041_001438"
     CDS             18563..19009
                     /gene="msrB"
                     /locus_tag="IV041_001438"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8382221.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19179..19781
                     /gene="ruvA"
                     /locus_tag="IV041_001439"
     CDS             19179..19781
                     /gene="ruvA"
                     /locus_tag="IV041_001439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8382222.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19794..20795
                     /gene="ruvB"
                     /locus_tag="IV041_001440"
     CDS             19794..20795
                     /gene="ruvB"
                     /locus_tag="IV041_001440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8382223.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20824..21402
                     /locus_tag="IV041_001441"
     CDS             20824..21402
                     /locus_tag="IV041_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8382224.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21454..21936
                     /locus_tag="IV041_001442"
     CDS             21454..21936
                     /locus_tag="IV041_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8382225.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22053..22427
                     /locus_tag="IV041_001443"
     CDS             22053..22427
                     /locus_tag="IV041_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382226.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7401 a         4069 c         4921 g         6357 t
ORIGIN      
        1 aaatgaaagc tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta
       61 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
      121 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
      181 aaaaaatcgg agcaactggt gattttttat tgaataatct agccatttta tttgttcctg
      241 ctggtgtagg tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg
      301 gagcagtcgt cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg
      361 tagaagcttt gctgggctat gtccgtaaga aaaaagcaga aagggtatct gtaaacgaaa
      421 tggaggagaa tcaaattgac tgagtttttt gtgaatccat tatttggctt aagtttgact
      481 ttaggagtgt acgtattatc tgacaaattt ttgcggaaat ataaactgcc tgttttgaat
      541 cctctcgtat tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat
      601 aaagactata atatcggggg caattttttg actatgctga ttactcctgc gacagtggca
      661 cttgccatcc ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg
      721 gctgcgattt tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc
      781 ttctctcttc ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt
      841 tctgtcgatt tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc
      901 tctacaggta tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa
      961 agtccagttg ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa
     1021 gcaatcgaga ttggtgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga
     1081 atattgaccg ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc
     1141 gaaaaccact attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag
     1201 acgtgtattt gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc
     1261 tcctcctaga aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg
     1321 ttcagctgga tgcccgcagc gtgtcgaaat acctgaacga gctgtctaaa aactatcagc
     1381 agtttagcgg taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt
     1441 atgatacgct cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc
     1501 tgaaattcca gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg
     1561 cgcaaaagca ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc
     1621 aactgcaaac ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag
     1681 aaatcattcg actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc
     1741 catttgaaac gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt
     1801 ttaatgttcg tctgaacgag atcaaaaaaa gacgattgga atatatgcta ggtgcctttt
     1861 ttttaagaaa atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt
     1921 ctgacaatct gttgttccag cgattttgcc aggtcatgga gccggtattt cctaattatt
     1981 ttcaagtaga agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt
     2041 atagtgatcc taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc
     2101 cctttacagc cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc
     2161 tgccagcaga aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg
     2221 cctatctttt tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc
     2281 atttagttat tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac
     2341 acaaacatcc caatcatctg gcatttaaaa atcatgcgtt tttgatgggg cggtatctga
     2401 ctgtgtttaa aacattagga gcctttactc cacagcttcc aaagatcacg atccttttaa
     2461 tgacagattt cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc
     2521 gtaatgagta tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc
     2581 tgatttcgac aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga
     2641 ctgaagaact aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa
     2701 aacaaaaact attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt
     2761 taccagaatt gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt
     2821 atgttgttct cgtatccgat gatgaatttg atggctgtcc ggtgacgccg tacttattga
     2881 tcaaaacaag tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag
     2941 atgattgggc gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt
     3001 tatcacagga gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga
     3061 tgcttttgta tttggattag atcaaaagtt cggtaaaatg gaagaaattt ctttgcgatt
     3121 tcctgaaact tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc
     3181 ggtatcgaaa tgccaaaatc attttggtga agttacgatt ttactgaata cagcggggat
     3241 attggatgcc tacaaaccct tgatggaaac tgacgaatcg ctgtgggatc tgatttatga
     3301 gacgaatgtg aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgcca
     3361 aaaaagcgga acgatcatca atatggcttc gattgctggc ttgattgctg gtggaggtgg
     3421 gatcgcttat acaagcgcga aacatgcgat tgtcggattt accaaacaac tcgctctgga
     3481 ttacgcagga cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat
     3541 gaatgcggct gattttgaag ggaaaggtga gatggcagag tgggtggcaa atgaaacacc
     3601 agtcaagcga tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc
     3661 tcaagcaagc tacatacaag gaacgatcat cccaatcgac ggcggatggt tgttaaaata
     3721 accttgtgtt tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg
     3781 ataaaaaaag tcgatgactg ttcccgacat tcgatgccga acagccagtt caaaaaattc
     3841 tttttaataa tcgttaatat tcgaaactaa gcggcttttc acgacctctt gatataaacg
     3901 atattcaaaa gcttgcaact gcggtaacaa gtccagccaa atgaaaaata taaggagcta
     3961 tttttatttg accattctta ttacttgttg cagggcagct ttttcactac cttctgcgac
     4021 acttcagttg gaattttctt cccatccttt ggtcgcggtc attaccaaga taaaattatc
     4081 ttgcaaagga gaaatagaac atggaaaaaa ataatacccg ttcagccaaa cgctggacaa
     4141 cgacagatgt gactcggatg gcgctagtca ctggtttata tgtggcagtg acgctggttt
     4201 tgtctgtctt tagctttgga gtgattcaga ttcgtctctc ggagatgttc aactatctgt
     4261 ccttgtataa caaaagatac atcgtcgcgg taacagcggg agtagctttg gctaatattg
     4321 cttctcctct tggactgata gatgttgcag taggaagcgt cagcacgctg cttgtattgc
     4381 ttatcaacta taaaatcact agtcgcatca aaaacatgaa aatcaaaatg gccgtgacag
     4441 catgcgtatt tgctttttcc atgtttacag tagctggtca gctaacgatt ttatatcaac
     4501 tacctttttt cttgaattgg tgggtgattg ctttaggaga attggcatca atgattgtcg
     4561 gtgggataat tatttattgg atcggtaaaa aagtggattt gtcaaaataa agatctgttt
     4621 aatttaaaat agggcagcat ggaagagact gaatgttgag tcttcttcca tgctgctttt
     4681 ttgattaatt tgtatgaaga aatcgattta gccgtttttt catcaagata ccgatataag
     4741 ctgctccagt tgccttgatt gcttccggca gtaaaaacgg cagtagtcca ctggcaaatg
     4801 cagttgtaaa agtaagatca ccacttattt tcagccagat cgttccgcag atcaatgcag
     4861 caagagagcc cattagattg gcaaagattg catataagta atgagcatcc gttctttcga
     4921 tcatccaacc agttatccac gtttgtaaga gaaatccaat caaatatccg ccagttggcc
     4981 caaataaaac agctatacct ccagtcatcc ctgaaaatac aggaagacca atcaagccaa
     5041 gtaaaagata aactccaaca gatatcaatc ctagttttct tccgagaaaa gttgctgtta
     5101 atccaatagc aaatgtttgc cctgtcaaag gaaccaaacc aaaaggaatg gtcatctgag
     5161 aaaggacaga aataatcgcg gcaaattccg cagcaagtat caattctttc aatgttcggt
     5221 tcataacgcc ctcttttcgt taacttaata tttttatttg gttaacgaaa ttgtaaaaca
     5281 aagtgattac tttgtaaata gaaaatataa aaaaagacca gctatccaat agatagcggt
     5341 ctttttcaag aaagcttttg aaattataag ctttggttgt agtattcaac gacaagagct
     5401 tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac gagtcaatga accttccaat
     5461 ttttcagcgt caaagcttac gaatgctggg cgtccaacag ttgcttctac agcttctttt
     5521 actgttacga tgttttgtga tttttcacga actgagatca cttgacccac ttctacgtgg
     5581 tatgaaggga tatcaacgcg tttgccatca actaaaatgt gaccatggtt aactaattga
     5641 cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa caacattgtc taaacgttgt
     5701 tctagtaaga tcatgaagtt aacaccatgt ttaccttctt tgattttgct agcttttacg
     5761 aacaagttac ggaattgacg ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca
     5821 gttaattgca tgccgtattc tgattgtttg ccacggctat taggtccatg ttgacctggt
     5881 gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca atgagatgcc taagcgacga
     5941 gaaattttcc atgatggtcc tgtataacga gacattaaaa tttcctccaa tgaatataag
     6001 tttttggagt aaaataatcc gttgaaaaat tcataatcat atagtttgtt cttcaatctt
     6061 cacctttgca gccgcggtta cacaatcgaa cccgagacgg ggcaacaaac tagggatgag
     6121 ttattatttt tctgctgcat tattttacac aaattacagt atatgataga tcatccacga
     6181 atgtcaagat aattagtaca taaaaacgct ttacaaacct aggctgtctt tataaaatga
     6241 gagggtatgt gcaaatggaa aaaagcgaag gaagtttaag gagaaaatca atgaatccgg
     6301 tatcaccatt atttgagaaa atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa
     6361 acagcttttc acgttacgct gttcgagcgg ttctcgcctg tttgtttttg actttaggaa
     6421 cggctgtggc atttgcgatc gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga
     6481 ttttatacgc atttatgttc agctggggtc tggtcatgat tctatacatg aacgccgaat
     6541 taggaacatc gaatatgctt tatatgaccg tgggtgtttt tcggcagaga cttgctttaa
     6601 gtaaggcagc caagattcta tttgcctgta tttttttcaa tatgattggc ggtatattat
     6661 gtggatttct aatttctatg actacgccat ttcaagattt aacgttggac aactttatgt
     6721 tagaatcaat agcagtaaaa ctagcaaaac cttcagcaac gattttggtg gaagggatgt
     6781 ttgcgaatat cgttgtcaat accgctgtac tgatcagcat gcggatgaaa gaggatgtcg
     6841 gaaaagtgat cacagtcgta ttcatcattt tcatttttgc ttttttagga tatgaacacg
     6901 tgatagcgaa ttttcctgcc tttacgctta cttactttgc ttctcacgga cagatggatg
     6961 gaatgacagt cagcaatgtg ttacataatt tgttttttgc tttggcagga aattatattg
     7021 gtggcggttt agtaatggga ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg
     7081 tggactaaaa agtcgttcat taaaggggaa gtgccatgga agttttagtt tttccaaaaa
     7141 atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa tcttttttcc tttgctggag
     7201 tacctaagcc ggaacgcgct cttttacaac aaccgttact ggatgagagc aaaaatttat
     7261 ctatttctga agttttagct tttgttcatt attattatga acaaatcgaa agagagtatc
     7321 agtatgtagc gatcgattta gtccagaaaa acatcaaacg gcttacattt gaagacttag
     7381 tcgtattgtt gccactagtt cagaaaaaag aatggtggga cagcatcgac tcttggagaa
     7441 aagtatacag cacatggtgt aaagcctacc cagagcagcg tgaagaagtc tttcaattgt
     7501 ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat cacgttgcaa ttaggttata
     7561 aacagcaaac agacacagga ctattgatcc gagcaataga cgaagatcgt caaacaaagg
     7621 aattctttat ccaaaaagca attggttggg cattacgcga ttatagtaag accgatgccg
     7681 attgggtaaa aagacagctg acaacagatt tgagtaagtt agctgttcga gaggcaagta
     7741 agtatttata agaaagcgag acgacaagag atgagacgca aattatttgc ttttgatatt
     7801 gatggaacgt tgttagattc aaacaaacaa gctttagata gcacaagaga agcattggaa
     7861 aaattacgca aagccggtca tctggtcacg attgctacag ggcggagccg atttcatgcg
     7921 caagagatca ttcgcgatct tgattttacg aactacattt tatgtaatgg agcggccggt
     7981 tttttagacc atgaacaagt atataagaat ttactagatc gcgaccagtt ggatgcgttc
     8041 gttcaagaag cgcatcatga gcagatcgat acagcttttg tcagcttaga caatatcaag
     8101 cgttcttctt ccaatgatgt aacggtgatg gaagaagcga tgcattcttt cggtgctgtc
     8161 ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa tataccaagc gttagcattt
     8221 ttcgatcaaa catatgatgg gaagttctct tcatatgata aaattcgatt tgtccgctgg
     8281 catgagaaca gtgtagatgt cgtacctaaa aatggttcta aagcagcgac aattctgcac
     8341 ttagcagaac aagtaggcat ccctcatgaa gacatcatca gctttggcga tggacaaaac
     8401 gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta tggggaatgc tgtgccagaa
     8461 gtacaagcag aagctaagat ggtcactgat acaaacgatc aggatggaat ttggaaggca
     8521 ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat
     8581 aagcccagcc aaacaaaaat agctcctcct atttttcgtt tttctgggct tattaccgca
     8641 gatgcaagct ttcgaacacc gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag
     8701 aaataagaag ggatttacga aaattgcttc tcaaattttt gtaaaattac ggcttatttc
     8761 tgaagaagta gatgaaacaa cgtttattcg ggaaatggga ctgtgacaag atgtttgtca
     8821 tagtcccatt tttttatttt atttttcaaa actaagaaat accaggaaag agttcctttt
     8881 gtttgacctt ttctttctta ttgaaaacaa ctataatata gagtgatgtt aaaagagaag
     8941 aggaatttag tgtgcgggta ttgtttattg gtgatgtagt aggttcaatg ggacgagaga
     9001 tgattactga atatcttcct agattgaaga agaagtaccg tccgcaagta acgattgtaa
     9061 atggtgagaa tgccgcttct ggacgtggaa tcacagaaaa aatctataaa aaatttttgc
     9121 aagacggcgt agatgtcgtg acgatgggga atcatacgtg ggacaaccgc ggaatctttg
     9181 agtttgtaaa tgaagctaaa aagatggttc gtccggcaaa ttttccggaa gggacgcctg
     9241 gacaagggat ggtctttgtc aaagtgaacc aagtcgaact ggcagtgatc aacatgcagg
     9301 ctcgttcttt tatggtggac ttggatgatc cttttcgtaa aatgaatgag ctggtagaag
     9361 aagccagaaa acgtacgccg atcatttttg tagattttca tggagaaaca acaagtgaaa
     9421 aacaagcaat gggctggttt ttagatggga aagtttcagc agtggtcggt acacatacac
     9481 acgttcagac aaatgatgcg cgaattttgc caagaggaac agcgtatctg acagacgttg
     9541 ggatgacagg tccctatgat ggtattttgg gtatgcgcag agaacctgta atcgaaaagt
     9601 ttctgactgc attaccaaaa cgttttgaag ttgtggaaca aggccgatcg attttaagcg
     9661 gctgtatctt ggaattggat gatacgacag gtcatgcaaa agaaatccag ctgattcaga
     9721 tcaacgatga tcgaccgttc atggagtaga aagagaggca gttttttgca gcaggagccg
     9781 gaaattcaaa aagaagtaag gaaactgaca aaacttttaa gagagaatga aacgatcatt
     9841 cgctacaaag aacttgaaga aaagatccag caaaaccagt atttagctga gttgagagaa
     9901 aaaatcaaac aagcgcaaaa agatgctgtt cattttgccc actatgacaa accagcagca
     9961 gaaaaagaag caatcaaaca agcggatcaa ttcatgcaag aatttgatca gcatccgctt
    10021 gttgtagctt atagaaaaca gctgttggaa gcagatgatc tgcttcatca tttaacaact
    10081 atgatacaag aagaaatcaa tggacaaata gaggaggaaa aacatgcctc aaaaaactaa
    10141 aaatactcct atgatggaac aatatctaag catcaaggcc caataccaag atgctttttt
    10201 gttctatcgt ttaggagact tttatgaact attttacgaa gatgcgatca aagcgtcaca
    10261 gcttttggaa ttaacgttga cgagcagaaa tcgaaatgca gaagaaccta ttcctatgtg
    10321 cggtgtacct caccatgcag cacaaggata tatcgatgcc ttgattgaaa aaggctataa
    10381 agtagcgatt tgcgagcagg tagaagatcc aaagacgact aaagggatgg tcaagcgcga
    10441 agtgatccag ctagtcactc ctggtacagt catggacagc aaaggattgt cggcaaaaga
    10501 caacaatttt ttaacagctg tagttagtca aggaaatgaa tttggctttg cttatgccga
    10561 tttaagtacg ggagaattga agacggctcg gttgcatgat gaagaagctg ttttaaatga
    10621 agcctctgca ttgcagacaa aagaactcgt attaggcagt gagatcactg atacccttcg
    10681 tgaacagcta agcgggcgtt tagggctagt cttttctcaa caaaatgaga tggaagaaaa
    10741 cgcggaattt agctttttga caagcgaact agtcgatccg ctggaaaaag aagcaaccgg
    10801 caaactattg acttatttat cggtgacaca aaagcggagt ttagctcata tccaaaaagc
    10861 agtagaatac caaccagatc attttttgaa aatggattat tattcaaaat tcaacctaga
    10921 attaagccaa tctattcgga ctgggaaaaa gcacggcact cttctatggt tgttagatga
    10981 aacaaaaaca gcaatgggtg gccgtttact aaaacaatgg ctagaccgtc cgcttatcca
    11041 ggcaaaacaa attaaagcaa gacaagaaat ggtcgcttct ttgctagaca gctattttga
    11101 acgtatcgat ttgcagtcag cattaacgaa agtctatgat ctggaacgcc tagcaggtcg
    11161 agtagctttc ggtaatgtca atgggcgaga cttgatccag ctgaaaactt ctttagaaca
    11221 agtgccaatg atcagagggt tgattgaagg aatcgatcaa ggtcaatggc aaagtctgct
    11281 ttcagaaatg cagccgatgg accatctcgt ccaattgatc gaccaagcta tccaagatga
    11341 tccgccgtta caaataacgg aaggaaacat catcaaagat ggattcaatg agcagctcga
    11401 tatgtaccgt tctgctatga gaaacggaaa gaaatggttg gcagaacttg aagcaaaaga
    11461 gcgtcaagaa acaggaatca agaacttaaa aatcggattt aaccgcgttt tcggttatta
    11521 tatagaaatt acaaaagcaa atcttggaaa tgcagaactg gagaagtatg aacgaaaaca
    11581 aactttagca aatgcagagc gtttcatcac accagaacta aaagaattgg agacacaaat
    11641 cctcgaagca gaagaaaaat ctgttgattt agaatatcag ttgtttttag ctgtgagaga
    11701 agaagtcaaa aaggctatcc agccgctaca agtacttgca aaagcaatca gtgcagtaga
    11761 tgtgctacaa agttttgcaa cgatcagcga acgctatcag tatgttcggc cagaacttgt
    11821 cagtgacaag catcagcttc tgatcaaaga cggacgacat cctgtagtag aaaaagtatt
    11881 aggacatcag gaatatatcc ctaacagcgt agaaatggca gaagacgaaa tgatcttgct
    11941 gattacagga ccgaatatgt ctggtaaaag tacgtatatg cgacagttgg cattaactgt
    12001 tctgatggcg caaatgggct gtttcgttcc ggcaaaacaa gcagttttac ctatttttga
    12061 tcggattttt acccgaattg gtgcaagtga cgacttaatt gctggacaaa gtacttttat
    12121 ggtagagatg atggaagcca atcaagccct gcgttatgct acgccaaaca gtttgatttt
    12181 gtttgacgaa ctagggcgag gaacagcaac ctatgacgga atggcgttgg cccaagcaat
    12241 catcgaatat atccatcgtc atgtacaagc aaaaacactc ttttctactc attatcatga
    12301 actgacggta cttgagaaag aacttccgca gcttaaaaat gtccatgttg gagccgttga
    12361 aaaagacgga gaagttgtct tcttgcataa aatgatggat ggacctgcgg ataaaagcta
    12421 cggcatacat gtggcaaaga tcgcaggatt gccttccaat ctactagaac gagcagcaga
    12481 tatcctgcat acgctagaaa atggcgataa cggacaccat actgttccag ctgaagtaga
    12541 agagaaaact aaaacagcca aacaggagca aataaaacca cctgcagaaa aagtaaatca
    12601 agaccatata caagaccgtg taaaagaaga aaaccaacag ctttctttat tcagtgaatt
    12661 atccgaaaat gaaacagaag tcattgaatc actgaagcaa ataaatttac tagaaatgac
    12721 gccaatggat gcactaaaca cattatatga attacaaaaa caactgtagg aagaaggcgg
    12781 atatcgatgg ccaaaatcca agaattatct gaacgtttag ctaatcagat cgctgcggga
    12841 gaagtagtgg agcgaccagc ttccgttgtg aaagaattag tggaaaatgc aattgacgcc
    12901 ggcagtactc agattgatat cttagttgaa gaagcgggac tgcggacgat ccaagtgatt
    12961 gataacggag aaggcatttt ggcagacgac gtagagaatg ccttcaagcg gcatgcgaca
    13021 agtaagatcc ataatcgcga cgatcttttt cgcatacgaa cgctgggctt tcgaggggaa
    13081 gctttgccaa gtattgcctc tgtttctgaa atgactctgg aaacggcgaa tcaggaagaa
    13141 aaacaaggca cctatctgtc gctgaaaggc ggagaagtta ttgagcatcg accagcacca
    13201 ctgcgtcaag gaacgaagat taccgtcagc aatctgtttt tcaatacgcc agcccgcttg
    13261 aaatacgtga aaacactgca cacagaatta gcgaacatcg gggacatcgt caatcgactg
    13321 gcattgagtc atccatcgat tgcttttcgt ttagtacatg atggaaacaa aatgctggca
    13381 actgctggaa atggcgatct gaaacaaaca attgcgggaa tctacggatt atctaccgca
    13441 aaaaaaatgc tgaaaatcga aggaaaagat ctagattttg agttatttgg ctatgtttca
    13501 ttgcctgagg tgactcgtgc cagtaggaat tacttatcga cgatcatcaa tggacgtttt
    13561 atcaagaact tttccttgaa taaagcaatc gttgcaggtt atgggtcaaa attaatggtc
    13621 ggtcgtttcc ctattgctgt attggaaatc aaaatggacc cgcttttagt agatgtaaat
    13681 gttcatccga cgaagcagga agtccggctg tcgaaggaaa aagaactaac agagctaatt
    13741 agtaacgcca ttcaaaaagc attgtctcaa gaaaatctga ttccaagtgc agcggataat
    13801 ctacggttta agaaaaaagt acctgatcag cctaaaacag aacaaatcga aatcgatttg
    13861 tcctacgctt ttgaagaaga aaaacctaaa aagccaagta gtttgaattt tgatcgagag
    13921 acaggtacct ttttcgtaga agaactaaag aacacatttg ttgacagaat tgttgataag
    13981 tctgtggaaa atgttgaaaa ctattctggt ttttctgaag aaaacaccac ttatccagaa
    14041 aaaacagcga aaacggtgga taagtttggt gaaaatgttg ataactttgt agaaaaaaca
    14101 gtggaaaaat ctgaaaagaa agaatttccc attgtggata acatggagaa aacaaatgaa
    14161 gaacaagcag ataaatccgt agaagaggat gaaaagattt acgaggaaga attgacactc
    14221 catcctgaat ttgatttcac tcaaggaaac gacaaaacat tagaacaagc catcaagaaa
    14281 ttagaaacgg aaaaaccagt acaacgtttt cctcagttgg aatattttgg acagatgcac
    14341 ggaacgtatc tttttgccca aagtagcgat gggttgtaca tcattgatca gcatgctgcc
    14401 caagagcgca ttaagtatga atatttccgg gaaaaaattg gggaagtatc tgatgatctg
    14461 caagaattgc ttgttccatt tgtgttagac tatccaaaca gtgatgcttt gaaattaaaa
    14521 gagcaaaaac acttgctaga agatgcaggg atttatttag aagaatttgg tcaaaacagc
    14581 tttattgtac gggcccatcc tacttggtat cccaatggag aagaagaatc gattattcgg
    14641 gagatgatcg atatgctgct gacaactgga agtgtgagtg taaaaaaatt ccgtgaagca
    14701 acagcaatca tgatgagttg caaacgatcg attaaagcca accattattt gaatgaagca
    14761 caagcacgtg tgctgctgaa ggatctggca cgatgcgaaa atcctttcaa ttgtccccat
    14821 ggccgtccag tattgattca ctttaccaat tcggatatgg aaaggatgtt caaacgaatc
    14881 caagacccgc attaagtcaa agagaagagg gaaaacatga agattgtatt agcatcccaa
    14941 tcaccaagaa gaaaagagtt attggctcat atcgtttcag aattcgagat tgaacctgct
    15001 gatatcgatg aaacgccatt tccagacgaa cagcccgtcg attatgttcg tagaatggct
    15061 gaagaaaaag cacgaactgt ctgggaacaa gatgaaaaag aagaccattt agtgattgcc
    15121 agtgacacga ctgttgttct caatcaagag atcatgggta aaccagaaga tctggctgag
    15181 gctgaattca tgctgaaaaa actaagcggg gaaacacata ctgtatatac agctgttgtt
    15241 ttaaaaacaa aggaacgaga agaacggata ctagcagaag cacatgtcac tttttatcct
    15301 ttgacagacg aagagatcaa acgctacttg gagacggggg attatgcgga taaggcaggg
    15361 gcatacggca tccaaaatgc tgcgagtgta ttcgtcaaag aaatttctgg agattattac
    15421 agtatcgtag gatttccgat tggtgctgtc aatcaggcac tgaaaacatt catttaaaca
    15481 aaaaagcgga ctatctgatt ttcgattgaa aatccatgat agcccgtttt ttttattgaa
    15541 aagattactt atctactctg actaagattt tacttcagct ttaccgactg cttcgcccgg
    15601 gcgaacaagt cctgattggt caagagaaag tcctgcaact ttttcattat tggtcaaagc
    15661 aacaataatg tcagttttct tgccagcttg ttctatttct ggcaaatcca tttcagcaat
    15721 cagggtctct ggtgtgacaa ggtatccttc tttcacgaat acattgaaag gaacaccctt
    15781 tagttcaaca gtatctaatc ccatgtgaac aagtacttcc agcccctcat tagaaagaat
    15841 accaatggca tgttttgttt caaagacact cgtcacttta ccgtttactg gtgcatagac
    15901 tttgccgttt gacggttcga cagcataacc gtcacccatc attttttgtg agaaaactgg
    15961 gtcatttact tcagttattt ccttgatggt ccctgcagcg actgcgaata atgtttcttt
    16021 tgttgcaact gccgtaccag ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc
    16081 gccatttgct gtttgaagtt gtacatcagc agcattttca ggaacagcag gatcaatctt
    16141 attcaaaatt ccctgacgtc taaagatgat cgtcaagcca aatggaatga tgacagcaat
    16201 gatcattgcg atgatgaaag gtacccatgt ttctgcttgg atagctagta ttcctggcaa
    16261 accgcctact ccgatcgcgt tagctcgaac acccatcaag ttagcaaaca tgccagctaa
    16321 tccagagccg accattgcag cgacgaaagg atagacgtat ttcaaattga ttccgaacat
    16381 agcaggttca gttacgccta agtaacaaga aatcattgct ggaatcgaga tttgttcttc
    16441 ttttttattc ccacgatgta agaagatgat ggctaagact gcagagcctt gggcgatatt
    16501 tgataaagca atcatcggcc ataaattcgt tgatccgaaa tctgcaatca gctgcatatc
    16561 gatggcattg gtcatatggt ggagaccagt aatcactaaa ggagcataca ggaatccgaa
    16621 tacggcacta aacaaccagc tgatagaaga cgtcaatcca gtatttacga tatttgagat
    16681 ccagcttccg actgtccagc cgataggacc taaaataacg tgtgcagcta aaacagttgg
    16741 taacaatgaa aacaatggaa caaaaatcat cgagatcgac tgagggatat atttgcggaa
    16801 gaagatttct agatacgcta acataaaccc tgccagcatg gctggaataa cttgcgcttg
    16861 ataaccaatc atctggactt gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc
    16921 tgtggaagct acactataag catttaataa ttgcggagaa actaacgtaa ttcctaaaac
    16981 gattcctaat atttgtgtag tacccatctt ttttgcaata ctccaagtga tacctactgg
    17041 aagaaaatgg aaaattgctt cacccggtaa ccacaaaaag gcattgacac cattccaaaa
    17101 cttcgagtgt tcaacgattg ttccaccaag actttcaaac tggatacctt caagtacatt
    17161 tctgaatcca aggatcaatc caccgacaat gattgcagga atcagtggtg taaagatttc
    17221 tgctaaaacg gcaatagcac gttgaacggg atgaaggttt tgttttgcag ctgcttttcc
    17281 ttgttctttg gaaacccctt caactccaga tactgcaacg aagtcgttat aaaaagtaga
    17341 aacatcgttt cctatgatga cttggaactg tcctgcattc gtgaacatcc cttttacgct
    17401 aggtatatct tcgatcgctt tttcgtccgc ttttcctgga tcattcagga caaaacgcat
    17461 acgagtcgca caatgactaa ctgcagcaat attttctttt ccgccgatat catgtaacag
    17521 cttctcggca tctgcttgat attttgccat atcaataaac ccctttctct taacttgtct
    17581 atgcaagttg tttcttcatt tactatactt tttttcggag agaattgcaa gcgttttatt
    17641 aaaaagtacg agagaaaatg atatcgtttt aaaaagctta aaataagctt ttcttaaaaa
    17701 taggaacatt gaaatacaaa aagagctgta gtatagtgaa gaaatgatgg aagaacgagt
    17761 gagggaaaaa acatgaataa gttcaatgaa atttttttag atttggaaca gaaaatttta
    17821 gcgaaagaat atcctccgca ttcgctttta cccagtgaga accaattgat caaaatttat
    17881 ggtgtttcac gagaaacaat tagaaaagcg ctgaatcttt tgactacgga aggttatatt
    17941 caaaaaaaac aaggaaaagg ttcgatcgta ctggatcgga atcgcttcga ctttcctatt
    18001 tctggattga ctagctataa agagctacag gagacccaac gtattccaag tgaaacgatc
    18061 atccatacgt tagaagaaac agaagtgaca aaagcattga acgagatcac tggctgggag
    18121 atcggtgcgc ctgtttggca tttgatacgc gaaagaaaaa tcaatggcga agtagttata
    18181 ttagacaccg attatctatt aaaggaaatc gttcctattt taactccagc acaagcacaa
    18241 ggatcgatct atgaatattt tgaaaatgag ctttctttga cgattgctta cgcgcaaaag
    18301 gagatcacag tagaagaagt aacggaaatc atgaaaacag cgatggatct tcacgatgac
    18361 acttatgcag tagtagtcag aagcctcgtt catctggaag atacccgctg tttcgagtat
    18421 tctgagtcgg ttcaccgatt agataaattc cgtttcgttg agtttgcccg acgccgaaaa
    18481 gtgtaaatag ttcaaaagaa agcaaattgt tggcatctgt ccactttggc gctatgatag
    18541 gatcacaaag ggggcatttt taatggataa accaataaga ccagaaaaag aagaactgaa
    18601 agaaaagtta tcacccatgg cttatgcagt cacacaagaa aatgcaacag aacgaccatt
    18661 tagcgggaaa tatgatgatt tttacgaaaa ggggatctat gtagatatcg tcagcggtga
    18721 accgttattc agctctgcag aaaaatatga tgcaggttgt ggctggccat cattcagcaa
    18781 accaataacg aaaagacaag tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat
    18841 agaagttcga agcaaagaag cagattctca tctcggacat gtgtttaccg atggaccagt
    18901 agatcaaggt ggtctacgtt actgcatcaa ttctgcctca ctgaaattca ttccatatga
    18961 aaagatggat gaattaggtt atggggaatt caaatcactt gtagaataat ggaaatacac
    19021 aaagagcggt acaatacggg gatttactcg atcgtccgct ctttttttat tcaagcgatt
    19081 gctatttgaa agaaacgctc cctagactcc ttttttcaca aggtgaaatg tgctacaata
    19141 aagggaacgt atgtgtgcaa aagaaagagg actagaatat gtatgaatat ctgacaggtg
    19201 tggttacctt tatcaacccc tattacatag tagtagaaac caatggtatc ggctaccaga
    19261 tcgcactagg aaatccttac cgctacagta gcaaactaaa tcaaaaaata aaaatttatg
    19321 tccatcaggt aatacgtgaa gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa
    19381 agcaactgtt tttgcgatta gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa
    19441 tcatggcaag cgaagaccac tccggtttga ttcaagcagt agaatccagt gatgtcactt
    19501 atttgacgaa atttccagga gtagggaaaa agacagcaca acaaatgatc ttggatctaa
    19561 aaggaaaatt cggtgagctg agtatcgata caccttttag tctatttgat gaagcaaaca
    19621 caaaagacgc tacagctcta tctgaagcaa tagaagctct ctcggctctt ggttacagtg
    19681 atcgagaaat caaacgtgta gaaaaacaat taaaagaagt agataatcag acaacggatg
    19741 agtatctacg ccaagcactc aagctaatga tgaaaaaata aggaggagac caaatgacag
    19801 atgaatactt gttatcacca gaatctggcg aaaatgaact gagtttggaa aaatctcttc
    19861 gaccacagct gctttctcaa tatatcggac agagaaaagt caaacaagaa cttcagattt
    19921 acatcgaagc tgccagaaat cgggaagaac ctttagatca tgttctgttg tacgggcctc
    19981 ctggattagg aaaaacgacg atggccatgg tcattgctaa tgagatgacc gtaaatattc
    20041 gtacgacgag tggccctgct atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac
    20101 tggaagcagg ggatgtcctg tttatcgatg agatccatcg attacctaga gtggtagaag
    20161 agatgcttta ttcagctatg gaagatttct atgtagatat catggtagga caagggccaa
    20221 ccgcacatcc ggttcatttc cctcttcctc cctttacttt gattggcgcg acgacacggg
    20281 caggtatgct ttctgcacct ttacgagatc gatttggcat catttctcat atggaatatt
    20341 atgacgaaca ggatctgcag gagatcgttt tacgttcttc ggatattttt caaacagaga
    20401 tcattgaaga aggggcattg gaaattgctc gccgatcacg aggaacgcct agaatcgcta
    20461 atcgcttgct caaacgggtt cgtgattttg cgcaagtcca aggagacggg aaaatcgatc
    20521 gtcctatagc ggatcaggca cttactttgc tacaggtcga tcaggctgga ttggattatg
    20581 ttgaccagaa attgttgcga acaatgatcg agttatatgg cggcggacca gtagggttaa
    20641 gtacgctttc agtaaacata ggagaagagc gtgagacggt agaagatatg tatgaaccgt
    20701 atctgatcca aaaaggattt ttaaagcgta cacctagagg gcgaattgct actgcttatg
    20761 cctatgaaca ttttggttac gactatcata tatgattttt gcttttagat tggaggaaaa
    20821 tgaatgagtg atacaacacg tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc
    20881 caaacgcaat cttttcgaaa aatcagcgtc caaaaaatcg caaacgaagc aaaaatcaat
    20941 cgacaaacat tttattatca ttttacagat aaatacgatt tgcttcgttg ggtctattgt
    21001 gaggatgcat tgcattattt agatccacaa tgtctttcac ttgataattg ggaagagcaa
    21061 gcattgaaaa tgctgaaagc aattgctgag aagaaagaat tttatgcaaa tactgtgctt
    21121 tctgacccag aaattttaca aaaagagttt tctcaactga tccaaaaatc atttatcaag
    21181 atttttgaac aaatggatga agaaagacaa ctttctgcaa gagacaaact attttatgct
    21241 cgtttttttt cttacggttg cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa
    21301 gaaacaccga tggaaatcgc tatgcagctt ttccgtttag ccaaggatac ggaattatat
    21361 tcttatcgtc tttatgcaaa agaagagttg gaagaacagt aatagaaaaa ttttttgttg
    21421 gaaaagcaat aatcgctaaa aggagatggg gaaatgacaa aaattttatt tgtttgtttg
    21481 ggaaatattt gccgttcacc gatggcagaa ggcttgctga gaaaacgaat agcattagaa
    21541 ggaagggaag cagaattttt tgtggattct gctgctacaa gcacgtatga agttggaaag
    21601 acaccgcacc ctggaacgaa aaaaatatta aaccaagaaa aagtagatat gacgaatatg
    21661 gttgctcggc aaatcacacc ccacgatttt gaaacttttg attggatcat tggcatggat
    21721 caagaaaatg tagaagaact gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat
    21781 ttgtttttaa gtagcattcc tggaaaagaa aaagaaaatg ttcccgaccc atactatacc
    21841 aataactttg atgagacata tcacttgatc acagaaggcc tgaatcattg gtggaatatt
    21901 tggattgatg aaaataaaat aacaaataat ttgtaaagtt aacgctttct ttttaaggcg
    21961 atgttagagc atttccttgt ttttttgtaa aatgttaggt aaaataaaat taattaagta
    22021 acactttctt agctaaagga ggagtgcatt cgatgaacga agagacagta tcgccaatga
    22081 agaagcttac gaagttagtt actcatactt cgtatcaact ttttgattct tttgtaaatg
    22141 acactgaaga acatgctaag aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa
    22201 aagctgctac tgaacatagt ctagttatct tacaagtaag agcaaatcgt ttgagtagca
    22261 agtatgaaac gatctcaggc tgggtggcga caaaaacggt cgaaaaagac cgagtgatga
    22321 tccgcctaca aaacgaagag cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa
    22381 taacaatcct ttcaccagca ggggaacagg aacggttatc aaaataattt ataccagata
    22441 gaaaagcacg tatgagcttg taccatcaat cattactaat caaaaataac cgaatgcttt
    22501 aagctgatat cagtaaaacg gaacgtttta ggatgagtat catagcatga cagcattcgg
    22561 ttgattagtt gaatcgattg gatggtacaa gtttttttga taaaaaaagg tcctgctagc
    22621 cggggagcta gcaggataag gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg
    22681 tatgcttata taatagtcat tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt
    22741 taagtttt
//
LOCUS       DACDCV010000038        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDCV010000038 DACDCV010000000
VERSION     DACDCV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.038"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV041_001444"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV041_001444"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8382227.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IV041_001445"
     CDS             complement(1172..1867)
                     /locus_tag="IV041_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8382228.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IV041_001446"
     CDS             complement(1880..2596)
                     /locus_tag="IV041_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8382229.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IV041_001447"
     CDS             2926..3534
                     /locus_tag="IV041_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8382230.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IV041_001448"
     CDS             3859..4182
                     /locus_tag="IV041_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8382231.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IV041_001449"
     CDS             4364..5734
                     /locus_tag="IV041_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8382232.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IV041_001450"
     CDS             complement(5864..7165)
                     /locus_tag="IV041_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8382233.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IV041_001451"
     CDS             complement(7519..9510)
                     /locus_tag="IV041_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8382234.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IV041_001452"
     CDS             complement(9654..9920)
                     /locus_tag="IV041_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8382235.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV041_001453"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV041_001453"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8382236.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV041_001454"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV041_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8382237.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IV041_001455"
     CDS             13264..13827
                     /locus_tag="IV041_001455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8382238.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IV041_001456"
     CDS             complement(14080..14601)
                     /locus_tag="IV041_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8382239.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IV041_001457"
     CDS             complement(14604..15446)
                     /locus_tag="IV041_001457"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8382240.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IV041_001458"
     CDS             complement(15439..16164)
                     /locus_tag="IV041_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8382241.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IV041_001459"
     CDS             16340..16636
                     /locus_tag="IV041_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382242.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IV041_001460"
     CDS             17204..18796
                     /locus_tag="IV041_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8382243.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IV041_001461"
     CDS             18809..19549
                     /locus_tag="IV041_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8382244.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IV041_001462"
     CDS             19717..21066
                     /locus_tag="IV041_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8382245.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IV041_001463"
     CDS             complement(21194..21784)
                     /locus_tag="IV041_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8382246.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACDCV010000039        21797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDCV010000039 DACDCV010000000
VERSION     DACDCV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.039"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1406
                     /gene="rseP"
                     /locus_tag="IV041_001464"
     CDS             138..1406
                     /gene="rseP"
                     /locus_tag="IV041_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8382247.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1474..3183
                     /locus_tag="IV041_001465"
     CDS             1474..3183
                     /locus_tag="IV041_001465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8382248.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3395..7753
                     /locus_tag="IV041_001466"
     CDS             3395..7753
                     /locus_tag="IV041_001466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8382249.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7898..8371
                     /gene="rimP"
                     /locus_tag="IV041_001467"
     CDS             7898..8371
                     /gene="rimP"
                     /locus_tag="IV041_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8382250.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8394..9569
                     /gene="nusA"
                     /locus_tag="IV041_001468"
     CDS             8394..9569
                     /gene="nusA"
                     /locus_tag="IV041_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8382251.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9591..9884
                     /locus_tag="IV041_001469"
     CDS             9591..9884
                     /locus_tag="IV041_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8382252.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9881..10192
                     /locus_tag="IV041_001470"
     CDS             9881..10192
                     /locus_tag="IV041_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8382253.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10205..12511
                     /gene="infB"
                     /locus_tag="IV041_001471"
     CDS             10205..12511
                     /gene="infB"
                     /locus_tag="IV041_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8382254.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12538..12885
                     /gene="rbfA"
                     /locus_tag="IV041_001472"
     CDS             12538..12885
                     /gene="rbfA"
                     /locus_tag="IV041_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8382255.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13042..13215)
                     /locus_tag="IV041_001473"
     CDS             complement(13042..13215)
                     /locus_tag="IV041_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382256.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13663..16025
                     /locus_tag="IV041_001474"
                     /pseudo
     CDS             13663..16025
                     /locus_tag="IV041_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16393..17316
                     /gene="truB"
                     /locus_tag="IV041_001475"
     CDS             16393..17316
                     /gene="truB"
                     /locus_tag="IV041_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8382257.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17320..18261
                     /gene="ribF"
                     /locus_tag="IV041_001476"
     CDS             17320..18261
                     /gene="ribF"
                     /locus_tag="IV041_001476"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8382258.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18613..19398
                     /locus_tag="IV041_001477"
     CDS             18613..19398
                     /locus_tag="IV041_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8382259.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19411..20262
                     /locus_tag="IV041_001478"
     CDS             19411..20262
                     /locus_tag="IV041_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8382260.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20386..20838)
                     /locus_tag="IV041_001479"
     CDS             complement(20386..20838)
                     /locus_tag="IV041_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382261.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(21303..21671)
                     /locus_tag="IV041_001480"
     CDS             complement(21303..21671)
                     /locus_tag="IV041_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8382262.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         7455 a         3809 c         4702 g         5831 t
ORIGIN      
        1 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
       61 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
      121 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
      181 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg
      241 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
      301 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
      361 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
      421 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
      481 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
      541 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
      601 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
      661 actttgctgg acccatgaat aactttattt tggcaatcct gttattcatc attctggcat
      721 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
      781 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
      841 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag
      901 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
      961 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     1021 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     1081 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     1141 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     1201 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     1261 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     1321 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     1381 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     1441 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     1501 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     1561 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     1621 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     1681 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     1741 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     1801 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     1861 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     1921 cttcgcggaa gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt
     1981 ttggacgaga catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc
     2041 ttagaatttc gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa
     2101 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     2161 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     2221 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     2281 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     2341 gtccttgttt tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     2401 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     2461 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     2521 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     2581 gtgaatccag aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa
     2641 ggagatcctt caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga
     2701 catatcttca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     2761 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     2821 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg
     2881 ccatttgatt tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg
     2941 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     3001 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     3061 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     3121 gttgaagtac gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa
     3181 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     3241 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     3301 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     3361 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     3421 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     3481 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca
     3541 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     3601 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     3661 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     3721 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     3781 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     3841 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     3901 agcggaacga gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     3961 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     4021 atcgccagct cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga
     4081 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     4141 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     4201 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     4261 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     4321 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     4381 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     4441 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg
     4501 acaccgagct atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag
     4561 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     4621 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt
     4681 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     4741 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     4801 tcctttgtca cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc
     4861 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     4921 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     4981 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     5041 attcaaacgt ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca
     5101 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     5161 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     5221 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     5281 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     5341 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     5401 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     5461 agcaaaattc tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     5521 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     5581 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     5641 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     5701 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     5761 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     5821 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     5881 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     5941 tgagatcatt caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc
     6001 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     6061 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     6121 taatccgtta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     6181 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     6241 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     6301 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     6361 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     6421 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     6481 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     6541 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     6601 atatccagca gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat
     6661 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     6721 gcttcaagat ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat
     6781 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     6841 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac
     6901 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     6961 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     7021 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     7081 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     7141 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     7201 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     7261 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     7321 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     7381 aatgaaagaa atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac
     7441 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     7501 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     7561 agctgtccca agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg
     7621 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     7681 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     7741 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     7801 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     7861 ggaattacgc acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt
     7921 cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt
     7981 tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga
     8041 tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc
     8101 agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt
     8161 gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca
     8221 accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct
     8281 tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat
     8341 tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta
     8401 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
     8461 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
     8521 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
     8581 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
     8641 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
     8701 ggcggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
     8761 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
     8821 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
     8881 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
     8941 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
     9001 tattgcgccg cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg
     9061 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
     9121 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
     9181 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
     9241 ttgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
     9301 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
     9361 aaaacgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
     9421 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
     9481 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
     9541 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
     9601 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
     9661 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
     9721 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
     9781 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
     9841 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
     9901 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
     9961 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    10021 aagcgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    10081 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    10141 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    10201 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    10261 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    10321 tagcaatgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    10381 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    10441 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    10501 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    10561 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    10621 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    10681 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    10741 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    10801 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    10861 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    10921 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    10981 agaagaagtc gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca
    11041 cgtagaccat gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg
    11101 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    11161 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    11221 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    11281 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    11341 aattgataaa ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt
    11401 aattcctgaa gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca
    11461 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    11521 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    11581 tcctgtgtca accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt
    11641 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    11701 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    11761 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa
    11821 acgtgcatta ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga
    11881 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    11941 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    12001 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    12061 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    12121 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    12181 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    12241 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    12301 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    12361 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    12421 tgaatgtggt gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    12481 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    12541 gctaactacc gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    12601 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    12661 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    12721 aaaacagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    12781 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    12841 ggcaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca
    12901 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    12961 agttccatga ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    13021 aaaaattcga ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa
    13081 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
    13141 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
    13201 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
    13261 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
    13321 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
    13381 cgatccagcc ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat
    13441 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
    13501 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
    13561 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
    13621 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
    13681 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
    13741 ctttatctta aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa
    13801 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
    13861 cgagtcatca ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga
    13921 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
    13981 acggaagatg agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt
    14041 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
    14101 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
    14161 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
    14221 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
    14281 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
    14341 cttggctggg aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg
    14401 gctgaaaaaa tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg
    14461 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
    14521 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
    14581 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
    14641 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
    14701 agagaaatcg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
    14761 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
    14821 ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
    14881 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
    14941 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
    15001 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
    15061 gggttcttag aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat
    15121 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg
    15181 acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc
    15241 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    15301 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    15361 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    15421 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    15481 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    15541 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    15601 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    15661 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    15721 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    15781 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    15841 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    15901 gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata
    15961 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    16021 aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa
    16081 aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt
    16141 tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc
    16201 aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta
    16261 cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg
    16321 tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa
    16381 tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat
    16441 gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg
    16501 cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc
    16561 gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca
    16621 acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct
    16681 gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca
    16741 atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa
    16801 acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg
    16861 acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga
    16921 acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg
    16981 tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa
    17041 gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa
    17101 ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac
    17161 ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct
    17221 ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa
    17281 ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga
    17341 catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc
    17401 gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa
    17461 gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag
    17521 atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc
    17581 gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca
    17641 ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt
    17701 tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc
    17761 aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc
    17821 tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta
    17881 ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta
    17941 ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt
    18001 tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat
    18061 aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat
    18121 caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg
    18181 aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct
    18241 gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc
    18301 aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc
    18361 acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa
    18421 atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact
    18481 agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg
    18541 tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
    18601 ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
    18661 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
    18721 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
    18781 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
    18841 gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa
    18901 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat
    18961 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa
    19021 aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct
    19081 cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat
    19141 gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat
    19201 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
    19261 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
    19321 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
    19381 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
    19441 gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
    19501 atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct
    19561 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt
    19621 attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
    19681 atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa
    19741 tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt
    19801 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt
    19861 agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt
    19921 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
    19981 atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg
    20041 atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
    20101 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
    20161 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
    20221 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
    20281 ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
    20341 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga
    20401 aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt
    20461 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
    20521 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg
    20581 attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag
    20641 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
    20701 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
    20761 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
    20821 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
    20881 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
    20941 gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta
    21001 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
    21061 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactatgta
    21121 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
    21181 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
    21241 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
    21301 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
    21361 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
    21421 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
    21481 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
    21541 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
    21601 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
    21661 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
    21721 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
    21781 taagatgtct caaataa
//
LOCUS       DACDCV010000040        21670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDCV010000040 DACDCV010000000
VERSION     DACDCV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.040"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(401..1426)
                     /locus_tag="IV041_001481"
     CDS             complement(401..1426)
                     /locus_tag="IV041_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8382263.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1701..3386
                     /locus_tag="IV041_001482"
     CDS             1701..3386
                     /locus_tag="IV041_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8382264.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3400..4683
                     /locus_tag="IV041_001483"
     CDS             3400..4683
                     /locus_tag="IV041_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8382265.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4753..5622
                     /locus_tag="IV041_001484"
     CDS             4753..5622
                     /locus_tag="IV041_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382266.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5619..6455
                     /locus_tag="IV041_001485"
     CDS             5619..6455
                     /locus_tag="IV041_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382267.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6481..7548
                     /locus_tag="IV041_001486"
     CDS             6481..7548
                     /locus_tag="IV041_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8382268.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7545..8372
                     /locus_tag="IV041_001487"
     CDS             7545..8372
                     /locus_tag="IV041_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8382269.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8417..9442
                     /locus_tag="IV041_001488"
     CDS             8417..9442
                     /locus_tag="IV041_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8382270.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9439..11622
                     /locus_tag="IV041_001489"
     CDS             9439..11622
                     /locus_tag="IV041_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8382271.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11622..12752
                     /locus_tag="IV041_001490"
     CDS             11622..12752
                     /locus_tag="IV041_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8382272.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12749..13426
                     /gene="pgmB"
                     /locus_tag="IV041_001491"
     CDS             12749..13426
                     /gene="pgmB"
                     /locus_tag="IV041_001491"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8382273.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13510..14037
                     /gene="msrA"
                     /locus_tag="IV041_001492"
     CDS             13510..14037
                     /gene="msrA"
                     /locus_tag="IV041_001492"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8382274.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14238..14924)
                     /locus_tag="IV041_001493"
     CDS             complement(14238..14924)
                     /locus_tag="IV041_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8382275.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15004..16542
                     /locus_tag="IV041_001494"
     CDS             15004..16542
                     /locus_tag="IV041_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8382276.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16662..17129
                     /locus_tag="IV041_001495"
     CDS             16662..17129
                     /locus_tag="IV041_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8382277.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17648..18091
                     /gene="rplM"
                     /locus_tag="IV041_001496"
     CDS             17648..18091
                     /gene="rplM"
                     /locus_tag="IV041_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8382278.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18107..18499
                     /gene="rpsI"
                     /locus_tag="IV041_001497"
     CDS             18107..18499
                     /gene="rpsI"
                     /locus_tag="IV041_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8382279.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18587..19813)
                     /locus_tag="IV041_001498"
     CDS             complement(18587..19813)
                     /locus_tag="IV041_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382280.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19885..20043)
                     /locus_tag="IV041_001499"
     CDS             complement(19885..20043)
                     /locus_tag="IV041_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382281.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            20735..21454
                     /locus_tag="IV041_001500"
     CDS             20735..21454
                     /locus_tag="IV041_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8382282.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21456..>21670
                     /locus_tag="IV041_001501"
     CDS             21456..>21670
                     /locus_tag="IV041_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8382283.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIV"
BASE COUNT         7021 a         3825 c         4491 g         6333 t
ORIGIN      
        1 caaaataaat tgatgggaag atagagcggt tatttttaat attttgctag ctattattga
       61 ataattcctc gaataaaaca aaaccagtag acatgtctgt tattctattt atgttttaga
      121 gataagtatc cccaaataac atggattgaa ataagggtaa atgtgtttaa aaaaacgaac
      181 tataaaaact tatacacaac gtggttaaaa atagtttttt cttccctgtg gaaaaaatag
      241 tttaacacaa acattatagt tatccacagc tatctaaaac agatggaaaa taagaaaaat
      301 tcgctaaaat accgggaggg ggtaacgagg gatgagtgat tgaaaaaaga acagagtaac
      361 tgacttatcc tcagtgtttc tgttctttta taattctgtt ctacatttct tgttgttgga
      421 tcgaatctgt gagagtaatc tcggttgaaa gaatgattct tttttcttca gggtattggt
      481 ggtggattag ttggaataaa agttctgtgg ctttgattcc taactgattt gtcggaatgt
      541 ctatccgagt caactgtggc gaaaataggg aggacaataa gggatcactg aatcctgcga
      601 tcgaaagatc atcaggtatc tgctttccag attgttctgc cactttgtaa atactcaatg
      661 attttaatgc atcggtagaa aaaatcgcag ttggtctttc tcgtagcttc attaaatcat
      721 tcattgctgc catgctatct tctaagcgaa agccgccgtc tataatccat tcattacgga
      781 tatctaggtg gtgatcaaat aagcattgac ggtaaccatt gagacgatca ttggatacat
      841 aaacttctgt cggagcatgg agacagccga tatgacggtg tccattcttg atcagtgttt
      901 ccaccaaatc gtaactatcc tggaaattat ccgtgtccac tgagtagatg ttcgtgtagt
      961 cgtttccgac ttttccgatt accacaatag gaatattata ttgatccaat tcaaaaagaa
     1021 gtgtttcatc catcgtagag cttaaaataa taattcctcg gatcattttt tcttggatct
     1081 ttttcgcgca tttttctagc tcttcttctg gtgtttttgc cgtttgtaga atcaagtcga
     1141 agttattttg ttcagaaacg gcagaaatgg attgaataac atctgaaaaa aaaggattgc
     1201 cataggtcgt atgagaagta cgatgagata atgctagaat cgcgtcaaaa ccatttcctg
     1261 acagtcctct tgcaattttg ctcggttcat aattcaattc tttgattgct tgaagtacac
     1321 ttgcttttgc tttatcagag atatttggct ggttatttaa tactctggag acagttgatt
     1381 ttgagacatt agcttttttc gcaatgtcat aaatagtagg tttcaatttt atatgcacct
     1441 cctttttggg agcgttttag aataattcca tagtacaata gacattcttt tttgtctatg
     1501 attttgaaaa tttaggaata gcttgcatga tcatgagaga ccatattttt ttaagaagct
     1561 attaattatt gatacataag ctttcttatt tgtatttttg tgtatatcaa aattatttaa
     1621 aaaaatgcta ttgacagtaa gcgttttcca taatatgatc aaatcgttgg gaacgctccc
     1681 aaaaaagaaa ggggaatccg atgtatacaa aacttttaca agcgatttat cgcgactcag
     1741 atttgtctga gaaaaccgcc tctatgattg aacaaaaagt cagtgtttat cagcagaaga
     1801 aaggccaggc aggcaaactg cattgggacg aagaagatat tttcttgatt acttatgggg
     1861 atcaattttt tgaagagcag caaccaactt tgcgtacatt taagaaattt tatcaaaagt
     1921 atttggcaga tacatttaga gttgtacatt tcttgccatt tttcccatat acatctgatg
     1981 atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg tttgggcaca tgggaagata
     2041 tagaagaaat gagaagcgaa acacggttaa tgttcgactt tgtctgcaat catatgtctg
     2101 ccgaaagtac ttggtttcag gaatatttaa acttgaatga tacgtacaaa gattttttta
     2161 ttgatttgcc actggataca gatacaagca gcgtgactag accaaggacc agcccgttac
     2221 ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt atggacgact tttagcgaag
     2281 atcaagtaga cttgaatttt aaaaatccag aagttttatt gaaaatgatc gatattcttt
     2341 tgttttactt agaaaaaggc gcttcattta ttcgcttgga cgctgtggga tttttatgga
     2401 aagaactagg aacttcttgt atacacctgc cagaaacgca taaaatcatc caattattcc
     2461 gtgcaatcgc tgatgaagca gctccaggaa cggtattgat cactgagaca aatgtgccac
     2521 acaaagataa tatcagttat tttgggaatg gcgagaatga agcacagatg gtctatcagt
     2581 tttcgctacc acctttgacc cttcaagcga ttcgttcggg taatacaagt aagttgaaag
     2641 aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac tttttttaat tttttagcct
     2701 cccatgacgg tatcggattg aatccggtac gaggtattct gccggaaaaa acgatccttg
     2761 aattagtgga acagctgaag aaagagggtg cccgagtaaa ctacaagaaa aacccagatg
     2821 gaacgaccag tccatatgag ataaacgcta cgtatctaga tgcgttaagt tctccaacag
     2881 atacgacgga atggagaagc aaacgctttt tagctgctca ttcatttttg gtaacgttac
     2941 caggcgtgcc ggcaatttat attcaaagtg tgctgggaag tcgaaatgat tacaaaggcg
     3001 tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa atttttgtta gcagatattg
     3061 agaaagagtt gcagacagat ccattaagaa cagcagtata tggtgaattg acccagatgc
     3121 tatctattcg aaaaaaagaa aaagccttcc atccgaatag tttgatttac ttactggact
     3181 cagcagattc ttttatcagt tttattcgcg aagcgaagga agagaaagtc ttagttatcc
     3241 ataatttttc caatcaatca gcaggttatc ttttaccaaa aggagattac ctagacctgt
     3301 tttctggaca aattatccat tgcagtcaag aacgaaagtt gatattccag ccgtatgaat
     3361 atatgtggct gaaaataata aaataagagg tggaaaaaaa tgaaaaaaat tattgttact
     3421 acctgtctgc tagctactgg atttcttctt actgcttgtg gtgaaagtca aagctctaac
     3481 gaatcacaac agacaacgat tcaattcatg cactcttctg tcgaacagga acgattaagt
     3541 gtgatcaatg aactggttgc tgattttgaa aaagaaaatc cagacatcaa aatcaagcaa
     3601 gtgccggtag aagaatcagc gttcaataca aaaatcgtta cgttagctcg ttccggtaaa
     3661 ttaccagaag taatggaagt cagtcaggat tttgctaaag tgatggataa agatgaactg
     3721 attgatcaga cggcagtcca aaacgttatc gaggaagctg gagaagataa ttattatgac
     3781 ggagccaaaa accttgttcg ttcagaagat ggaaaaagct atattgctgc accaattagc
     3841 ggctgggttc aaggtatttg gtatgacaaa gaaaaattat cagaagctgg tttttcagaa
     3901 ccagaaaatt gggatgatat tttaaaaatt gctcaacatt tcacagataa agagaataaa
     3961 caatatggaa ttgctatgcc tactgctgaa ggaacaatga gtgagcaagc gttctctcaa
     4021 tttgctttgt caaatggagc aaatgttttg gatgatgaag gcgaggtaac gatcgatacg
     4081 gaaaagatga gagaagcatt gtcgttttat caaaacttat cccaatacac gatgccaggc
     4141 tctaatgatg tgacagaaat caaagatgct ttcatgaatg gaacagcacc aatggcaatc
     4201 tattcaactt acattttgcc ttctgtttat gaagcgggga actcagaaaa tatcggtttt
     4261 gctatcccaa cacaaaaaga tcaagctgtc tatggcacag tatctggatt gacgatttct
     4321 tctggtctag acgagaaaca aaaagaggct gctgaagcat tcacagcttt cttatctcaa
     4381 ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg gtgctcaacc agtgaataaa
     4441 caagtagtcg aactggatgg ttacaaagaa aatgaagtca tcaagtcttt tggcgagctg
     4501 ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct ttggactagt cggggaaaag
     4561 aatttcacga aaatgggaga tatcacaagc tcaggtgtga tcggaaaagc agtgaaccaa
     4621 gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg acgcacaaaa aaatacgaaa
     4681 taacaagaag ttctgccaat ggtgttcgaa aaaagtagat agctattggc agattatcag
     4741 ggggaggttt caatgaatcg tccaaggaaa cattctgact taaaattagc catgggactt
     4801 ttagcaccaa gcatgctttt attagctgtt ttagtgctga ttccaatgct ttccaatatt
     4861 cagatcagtt ttttcgatat gccgctgaat cctaactcag acgccgtttt tatcggatta
     4921 agcaattatc ttgaaatttt aagtgatagt gaattttatc gttctttagg tttaacagtt
     4981 gcttacacat tcttagttgt agcaggcagt acagctttag gattagccgt agctattttg
     5041 cttaaccgca agttcagatt taggaaaacg gtgcgttcac tgattttatt aagttatgtt
     5101 acgccatcta tttctttgat ttttgcttgg aaatatatgt tcaataatag ttatggaatc
     5161 gtcaatgatc tgttagtaga taggttgcag cttttcaacg aagcaccgct gtggttcgac
     5221 cagccagttg ctagcttctt tatggtcgtt ttgtttgcta tctggagata ctatccgtat
     5281 gcgtttattt catttttggc tattttgcaa acagtcgaca aaacgcttta tgaagcagct
     5341 gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg tgacactgcc ggcaatcaaa
     5401 ccagtactag caactgtgat tgttttacga tctatctgga tgttctatat gtttacagat
     5461 gtctatctac tgacgaataa agtcaatatc ttaggcgttt atttgtatca gacagccttt
     5521 gcgttcaatg atcttggaaa agcagcagct atatcgattc tattgttcat cattattgca
     5581 gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat gaaaaataaa aagagcagga
     5641 aaatcttatt ttacgcgacg attttgttgc tattagctgt caatttgttt ccatttttga
     5701 tcatgctgat gacatcattc aaggattcac aagaagcaat tgcaacgaca caaactattt
     5761 ttccgaaaga atggactttt gaacattatc aggatatttt caatgctgag atttttccat
     5821 tcatcatgta tttcaaaaat agtttagtga tctccttgat agcctccgta actgcagtag
     5881 tgattgggat cagcggggca tacgctttgt caaaactacg cttcaaagct cgctcgaaaa
     5941 tcaatgctag cttttacacc gtttatatgt tttccggtat tttgatcgtt gttccattat
     6001 ttaaaatcat ttccggtcta ggtttgtatg ataccaatac agcactgatt atcacgatgg
     6061 tcgttcaaac acttcctact gcgatattca tgctaaaaag ttattttgac acgattccga
     6121 ctgatattga agaagcagca atgatcgatg gtcttagccg gatccagatc attattcgga
     6181 tcattatacc attggctatt tctggaatca tttctgtctt cgtttattgc tttatggttt
     6241 cgtggaatga ttatttattt gcttcgattt tcctgtcgac ctccgaaaaa tttactttac
     6301 caatcggttt gaacacatta ttcagtacac cagactacat ttggggacga atgatggccg
     6361 cttcattggt gaccgcttta ccagtcatca ttatgtatgc gttatctgaa cgatttatta
     6421 aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga aaagaaagag gaaagaaaag
     6481 atgaaaaaat tagttgcatt agaaccaaga atagccggat tcaaagatta cgaagaccgt
     6541 gagatcgaaa agaacgaagt acgtatccga gtaattttcg ctactccaaa accaggaaca
     6601 gaaattgtcg actttagagg attaagtcca tttatcgatg aagaattttc agaagaatgg
     6661 cgcctattct tgccaagaga agaaggaagc caacgcggtg tggtatttgg cgaatttcaa
     6721 ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca gtttggtgac agagtatcaa
     6781 aaaggagagt tagtctgcac ttatggaccc attcgtgaaa cgctgattac aaacggtgtt
     6841 gataattata aactacgtaa acttgatcgg ttggatcaat ggaaaaatgc agtatgttac
     6901 gatcctgctc aattcgcttt aggtggtatc cgggaagcac atgttcgagc aggtgatgct
     6961 gtagcaatct tcggtttagg cgctatcggg caaattgcga ttcaattagc taaaaaagca
     7021 ggtgctacta ccatcgttgg gatcgatccc atagcgcagc gtagagaatt agcagaaaaa
     7081 cacggtgccg attacacgct agatcctatg aaagcagatg tcggttttcg tctaaaggaa
     7141 ttgacagata aacgtggagt agattcagtt attgaaacga gtggcagtgc tgccgcatta
     7201 caagcttctt taatagggat cggctatggc gggatcattt cttatgttgc ctttgctaaa
     7261 ccttttccag ctgggtttaa tcttggtcgc gaagcacatt tcaataatgc aaaaatcgtc
     7321 tttgctcgag catctagtga accaaaccca gattacccaa gatggaaccg taaacgaatc
     7381 gaggacacat gttgggaatt attgatgaac ggttacttgg attgtgaaga aatcattgat
     7441 ccagttgtgc catttgcaga aagcgcagaa gcttacatgc gttatgtaga ccagcaccca
     7501 gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag aacaatgaaa ttcggtacgc
     7561 aagatcaagc atttttccca agagacattt tagaaaaatt tagatttatc aaagactgtg
     7621 gatttgaaga atacgaaatc gacggtaagt tgttaataga aaatatcgac acagtcaaac
     7681 aggcaatcaa agaaacaggg atcccagtag tgacagcttg taacggctac gatgggtgga
     7741 tcggtgactt tatcgaagaa cggcgtctta atggtgtacg tcaaattgct gagattttgc
     7801 gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc agcagcttgg ggaatgttca
     7861 cttatcgatt accgccgatg gtttcacccc gaagcaaaga aggagactat aaagcagtca
     7921 gcgaatcact tcgttattta gataaagtag ccgaagaaac aggtacggtt atttatttgg
     7981 agcctttgaa ccgataccaa gaccatatga tcaatttgtt agcggatgcg cgtcattaca
     8041 ttgactctaa tcaattgaaa catgtgcaga tcatcgctga tttttatcat atgaatattg
     8101 aagaagatga tatttcaaaa gcattgacta cgcaaaaaga gtatgtgggt catatccatt
     8161 tagcagataa tcatcgctac caaccaggat ctggcgcaat tgattttact cgccattttc
     8221 aaacgctact tcaaaatgag tatgaaggtt atatggtata tgaatgtcgt ttgcgaggag
     8281 aaaatgaagt gaaaatttat caggaatcat tgcaatactt gaaaaactgt ctggaaacag
     8341 cgcgtcattt aaatgattta gagaaagaat agagaaagaa taacaataag taaataagca
     8401 aaggagagag aaataaatga agaaagtagc aatcataggc gctggacaag tagcagaaaa
     8461 agtacatgct gcttattatc aaaaaagatc agatgtctta gaattggccg ctgtggttga
     8521 tccagtcagc cagcgagcag aagacttttg ccgacgtaca ggattttctc atgcgtatac
     8581 atcagttaca caaatgctga atgagatttc tcccgatatc gtgagtgtct gtacacccaa
     8641 tcgctttcac ttcgaacatg tattggctgc cctcgaagct aatgcttcag tgatgtgcga
     8701 gaaaccacca gctatgacag ctgatcaagc aagaaagatg gcagagacag ccgataaaaa
     8761 cgggcagatt ttggcttatg gattccagca tcgtttttct gacgaagcaa caatgatcaa
     8821 gaatcatctt gatgttctcg gggatattta ttacgtagaa gcgaatgcgc ttcgtcggtc
     8881 tggtgttcca ggctggggaa cgtttatcga caaagaactt caaggaggcg ggcctttgat
     8941 cgattatggc attcacatgc ttgatacagc gatgtatatt actggatttc ctaaagtaaa
     9001 aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa aaaaattcag ggaaattcgg
     9061 gaaatgggat ccaagcaaat tcacagtaga agatgcaatt tttggcacac tggaattaga
     9121 aaacggcaag atcatccaac tgaatacttc gtttgctttg aatatcagag aagacaaact
     9181 gaatgtggct ttttgtggcg atcaggcagg tgctaatctg tatccaggcg aactattcga
     9241 agatgtagat ggaaagctgg tttccttaca aaagaccacg ccagttaatg acccacatga
     9301 actaagtatg gctcattttg tgaatcgggc gctcggtgac cagactcaga tgattgcaga
     9361 tggctggcaa ggatatgtca tccagaatct ggtggaaaat ttatatcgtt cggcagaaac
     9421 aggagaagtt attaccttat gaaagtagaa gaaaaacagt ttgatttaga gtatatcaat
     9481 aagtatgcga gtcagatgac cattggaaat ggattttttg gtatcagagg aagccaggag
     9541 gaagactatc ggaatcaagt aaggggaatg tttgcagctg gtgtatataa ccgaccttta
     9601 ggagcagaat cagccgagtt agtgaatctc ccagatgtca tcagatggga aataaaaatc
     9661 gacggtgaac atttttctct tgagacgatg aaagttcttg catacgagcg ggcactagat
     9721 ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa ctgctcaagg aaaaaaagtg
     9781 gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc atcaggcagc cctgaaagtg
     9841 gtcatcaagc cgttgaccga agactgccaa gtagatgtga aaacgggtat cgatggacag
     9901 caaaccaatt ttggtacaca gcagttggtt gaaaaagaac tgcgagtatt tggtgacgat
     9961 ttgatcagtg catcctacca gacaacagaa agtaaaattg atattgggat tgcagctaaa
    10021 ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga tcaccgcttc cttttcagaa
    10081 aagatcagaa aaaatacgtc ctttacgctg gagaagatca tcgtgctctc tacctctctt
    10141 cagcaaacag acccgtttaa gaaaagtatt gcccaagccc agaaaagcgt tagttataca
    10201 gaaattttag agcagtcgat gtcttattgg acagattttt ggcatcagca tcggataacg
    10261 atccaaggcg attcttcttt cgatcagtta gcgatggact tcgctgctta ccatttacag
    10321 attatgactc cccgcaatga tccaagtgtt agtgtagggg cgaagggact aactggcgaa
    10381 ggttataaag ggcatgtttt ctgggatacg gaaattttta tcctgccttt ctttttatat
    10441 cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt atgatcgatt agaaggcgca
    10501 cggcaaaaag caagagatta tcactatcaa ggtgcacttt tcccatggga aagcgcacac
    10561 actggtcagg aagagacacc caaatttgct gcattgaata tcaaaaccgg aacaagacaa
    10621 gaagttgctt cagctgccgc agaacatcat atcagtgcag accttgctta tgccgttcaa
    10681 gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa caaagggaaa aacgctcatt
    10741 catgaaacag cagctttttg gctgagcaga gctgtaaaag tcgatgatca tttagagata
    10801 cacagagtca ttggtccaga tgaatacaca gaatatatcg acaataatgc atatacgaac
    10861 tatctggcgc attataatgt ggccttagct gttaaatatc aagctggcga tccgcttttt
    10921 ttgaaagaat gtcaaaattt tttagataag ttatatttgc cagtgcctaa caaagaacag
    10981 ctgattccgc aagatgacac gtatctcagt aagccagtta ttgatttgac cacttacaaa
    11041 gaacgtcctg gcagccagtc tattttactt gattatgccc gtagcgaagt aaatgaactc
    11101 caagtcagca agcaagcaga tcttgtcatg ctgttatact tattacctga gtatttttct
    11161 caaccaatcg tttctaagaa tttgcattac tatgaaaaca aaacaatcca cgactcatcg
    11221 ttgagtaaag cgattcacgc catagaggct tgccgtatcc aagaaatgga atgggcgtat
    11281 catttattcc aagaagcgtg cctgattgat ttaggaccag atccgcattc atcagatgac
    11341 ggaattcatg ctgcttcatt aggagcacta tggctagcag ttatatttgg tttttcaggt
    11401 attacagtaa cgaatggaac tctttctatt tctccaagac ttccttcagc atgggaaaaa
    11461 atttcttttc cttttatttg gaaaaatcac aggttgctat ttgttttgac aaaaaaacaa
    11521 atagtaataa aaaaagagac gaaagaaaca ttacctattt ttgtcaatgg agaccaatac
    11581 cagctggaaa aagaactgat cttggaacgg aggggaaaat aatgcgtatt ctgattgcgc
    11641 cagattcttt caaagaaagt ctgacagcct tagaggcagc acaagccatt caatcaggtt
    11701 ttcaagaagt ttttgttcag gctgaatatg atctgctacc tataggcgat ggaggagaag
    11761 gaacggtttc atcattagca gacagtttaa ggttgaagcg gcaaacagca gtcgttacta
    11821 atgcatttgg caaaaacaaa gaaatccagt acgcttcaga cggtcaaact gcgatttttg
    11881 aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa ccaacaacag ccacttaggt
    11941 tatccacgcg aggagtagga gaactgatac gacaattgtt gcaacaaggt gttcgacaca
    12001 tcatcatcgg cgttggcgga agtagtacca acgatggcgg catcggcatg gcagaaggtc
    12061 taggttatgc tttttacgat caagataatc aaaaagtaga agcactggga gaaaatctag
    12121 gtaaaattgc tacagttgat aaaacgaacg tgcctattga gctgattaaa gccaaaatca
    12181 ctgtcattac tgatgttact aatcttttat gcggaaaaga aggcgcttct tttgttttcg
    12241 gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc tgaccaagcc atgcatgatt
    12301 tttatatgag atttgcccct caagtaatcg aggcatcggg cagtagtgct ggtggtggaa
    12361 tgggtgcagg actgttagca tttgcttcag ggaaaatagt ctctggcatc gactacgtat
    12421 tggatttact ggagttcgat aaacgggtac agcaagcaga cctcgtaatc gtaggcgaag
    12481 gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt tgggattgct agaagagtac
    12541 cagagggcgt tcctgtgata gctatttgcg gaagtgttgg taaaggaagc gaaaaagcag
    12601 cagatgttgg catttcggcc gtttttccga tccttcctgc agctgtgcct ttacaagaag
    12661 ctttacagca agccactgat aatcttcatc gcacagctaa aaacatcgca tctttacttc
    12721 aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa gcagcactat tcgatttaga
    12781 tggcgttctt gtagacacag ctcgttatca ttacgaagcg tggctggttt tagctaacca
    12841 gctgtccatt ccattcactg agaaagaaaa cgagcaactg aaaggaatat ccagaaccga
    12901 gtcactagag cgattgctgt catttggaaa aatggaacag aaattctcag aaaaagagaa
    12961 atcagccttt gctgagcaaa aaaacaatct atatcttcaa gcgattcaaa aaatggatga
    13021 aacatccgtt ttgcctggtg caatagctgt tcttgagtac ttgaaaaaaa caaacatcaa
    13081 gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt ttagaaaaaa cgaatttgac
    13141 aagttatttt gatgtcctta ttgacggcac gcaagtatca aaagcaaaac ctgatccaga
    13201 agtttttctc aaaggagcac agcaattgaa tgtaccgcca aatgcatgct tggtcattga
    13261 agattcagaa gctggttgtc aagcagcttt ggctggtaac atgcatgtat tgggaatagg
    13321 tgaaaacata aatcttcctt ctgctgaata tgtgattcct gatttaaccg tttttgatca
    13381 agtacgttcg ttttggcatt tatcagaagc cgtatcacgt atataatagt ttccgttttt
    13441 tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa ggtatgatga gaaaaaaggg
    13501 ggattttata tgacaaaaaa agcagttttc gcaggcggat gcttttggtg tatggtcaaa
    13561 ccatttgaag agcagccagg gatcatttct gtgacatccg gttatactgg cgggcatgta
    13621 ccaaatccaa cttatgaaca agtaacaaca ggcacaacag ggcatacaga agcagtagag
    13681 atcgaatatg atccagaagt tatttcttat gatcagttgg tggaaatcta ttggcagcaa
    13741 accgatccga ctgacgcatt aggacagttc gctgatcgcg gtgattctta tcgtccagtg
    13801 atctactata atgatgaaga agaacgaaat attgcagagc gttcaaaaca agcattgcaa
    13861 gacagcgggc gattcgataa gccaatcgtg acaaaaatcg aaccaagaag cgaattttat
    13921 cctgcagaag attaccatca ggattattac aagaaaaaca agctccatta caatctttat
    13981 cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt ggggcgtcaa gaaataagaa
    14041 gccgaaaaag ctacttatgg aacaccgtat atcagaggtt gtaaaaccag cattttgagc
    14101 taatactgag ggtcagctga ttgaacacca tttagtggag aggttgtgaa cctgtcgcct
    14161 agctccaagt cacaaggatt tggtaagaga acactatata tcagaggttg tgacaagtcg
    14221 tttgtcacaa ccttttttca atacctaaga tgaaggacaa tcagcaacgc actgctcaaa
    14281 aaaggaatga aaggcagctt ttccaacttt tttttatgac agataaagta aacagaaaaa
    14341 acggatattc caaacagact agcaagaaac aagatctgaa gcaactcttc tagagacaag
    14401 ccaccactcc aagaaagcat caacagagta tcgccagctc cgagatagga ttgaccatag
    14461 cgaaggcaca caccgcctac gataaacgtt aacagcacac tttgccattg gaagacgccg
    14521 gcctgcaacc aaaaaatcca taaaattgcc caagaacaga atagcgtgtg agaatggacc
    14581 atgaagtaaa ataaatccat caacgaaaat agcaaagccg aaagcatcca tatgaacgtc
    14641 gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa aaagaccacc acataaggct
    14701 tctgccaaca aatagtagaa gggcaaccga cacctgcaat agcggcagcg aaatcgttgg
    14761 acgatgatcg agagaatcgg aatcaattca taccaagaaa gcgaacgaca gcatttgaca
    14821 caatgagatc taggataaat aatagagtga cctaacggaa tccgctgggc ggttaaacat
    14881 aaaaatgatc cgatacagca accaatgata aaataaagta acacgccatc tccccctttt
    14941 caaaaccaag atacgcaagc ttaaactaaa tttttgtaca tacataaaat ttgaggtgaa
    15001 aaaatgaacg tttcaatcgg attagatatc ggaaccacac aaaccaaagc agttgctttc
    15061 acagatgaag cccaagaagc agcatccgct tattttcgct accctttgat ccaagaaaca
    15121 caagggatgg cagaacaaga tcaggaattg atctttcaag gtgtctgcac cgttataaaa
    15181 gaagtaatta aacagttgcc ttcagacaca atcattcaat ttgtctcctt ctcctctgcg
    15241 atgcatggcc tgatattgtt ggatgaagag aaaacaccgt tatctcgaat gatcacttgg
    15301 gcagataacc gagcagcgga cgaagcagaa aaactgaaat ataccgtgct tggcaaagaa
    15361 ttttatcggc agactgggac gccgattcat ccaatgacac cattgaacaa aatcaagtgg
    15421 ctgcaaaaca gccaacctga attattcaaa aaaactgctt attttgtcgg tatcaaagac
    15481 tatatctttt atagattgtt tggcgaactg atcagcgact acggaacagc ttcaggaact
    15541 ggatattttg atatccatga attccaatgg gcagaagaag tgcttgacta cttgaacatt
    15601 cgaatagagc aattgccgca agtctttcct tccacctatc aagtaacagg tctaccatca
    15661 aaaacagcag cagaactagg tttgccaaat gatatatcat ttgttttggg aggggcagac
    15721 gggccgcttt ctaatctagg gttaggtgct tttggtgata cgatcgccgc tttgaccgta
    15781 ggaacgagcg gagcacttcg ttttattact actcagcctc agttccatcc agaaatggag
    15841 acgttttgtt atgttctgga tcagaaacat tgggtagtgg gaggagcgac tagcaatggt
    15901 gctggcatct ttgattgggc agggcataca tttttacagg aagtcgttca atctgctaaa
    15961 gaaaaaggag aaaatccata tgatgcactg ctttcggcta tcgcttttgt gccagcgggt
    16021 gccaatggtc tgatttttca tccctattta cttggtgagc gagcaccgct ttgggacgca
    16081 gatgcgtttg gcagttttat cggtttacag cggcaacata cagaaaaaga aatgatgaag
    16141 gcgattctag aagggatctg tctcaacctt tatcggattt taaaaggagt ttgccaggaa
    16201 aatcagctca cagagatccg tgctacaggc gggtttgctc aatctccgat ttttcgtcag
    16261 atgatggctg atgtactggg ttacccgctt gttttcccta ctgtcacaga agcatccgca
    16321 ttaggagctg tgattttagg ctggagaagc atcggaaaga tccaaagtat cagtgaagca
    16381 caggaattga tagaattgaa agagcaagtt ggagtacaag aaactgatca taaaaaatat
    16441 cagaaaatct atccattatt tgtttcgaca caaaagcatt tagcacagac ttatcagcaa
    16501 tttgctcaac tgcgagaaga attagctgat tctgaaaaat gattggcttt acggataaaa
    16561 aagcgaactg aaaatcgttc tcaggtaaat gattttgttg tacttttcat tgaaagtcta
    16621 ctattatttt aagtgtaaga aaatgttagg aggcaagaaa gatgaaattc caacaaacca
    16681 aagaagtatt aaatcaatta gttgcagatc taagccaaat gtctgttgtt gttcaccaaa
    16741 cacattggta catgagagga ccaggtttcc ttacattgca cccaatgatg gatgagttca
    16801 tggatgattt gaacgctcag ttagatgaag tatccgaacg cttgatcaca ttagacggtt
    16861 caccttattc tacattgcgt gaatttgcag ataacacaaa aatccctgat gaaattggcc
    16921 gctgggatcg tactattcca gaacgtttgg aaacacttgt tgcaggctat cgttatctag
    16981 cagatcttta ccaaaaagga atcgaagttt ctggagaaga aggcgacgat ccaacacaag
    17041 atatctttat cgaattcaaa acagccacag aaaaacgtat ctggatgatc caagctcatt
    17101 tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt gaacttgacc gaaaaagagg
    17161 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acgtcaaaat agttcctcat
    17221 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag
    17281 gttgtaagcc tgccgttcag ctccgagtca caagaaacaa ttttaaaaaa cagcttctta
    17341 tgttttatgg atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcaa
    17401 aataaggggt tgtgacaaga cttttgtcac agcctctttt tgtttttcat tttgacagga
    17461 aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt tcatataaat ttttcaatca
    17521 agcagaaaaa cgttgacatt gactagatta gcgggtaata tgttaaatgg tattgtttgc
    17581 cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca tcagaactta aattggagga
    17641 aaataacgtg cgtacaacat atatggccaa accaggcgaa gtagaacgta aatggtatgt
    17701 agtagacgca actgatgttc cattgggacg tctttcaaca gttgttgcat ctgtactacg
    17761 tggtaaaaat aaaccaactt tcacacctca tttggatact ggcgatttcg tcatcgtcat
    17821 caatgcagac aaagtgaaat taacaggtaa aaaagcgact gacaaaattt attaccgtca
    17881 cagcaactac ccaggaggat tgaaatcaat cactgctggc gaattgcgtg ctaaaaattc
    17941 tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct aaaaacactt taggccgcaa
    18001 acaatttaca aaattgaacg tatacggtgg agcagaacat ccacatgcag cacaacaacc
    18061 agaagtttta gacatcacaa acttaatcta ataggaggga atttcattgg cacaagttca
    18121 atatatcggc acaggccgtc gtaaaaacgc agttgctcgc gtacgtctag taccaggaac
    18181 tggcaaaatt actgtaaata aaaaagatgt tgaagaatac attccacatg ctgacttgcg
    18241 tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa ggcgcatatg acgtatttgt
    18301 taacgtaaac ggcggaggat acgctggaca atcaggagct atccgtcatg gtatcgctcg
    18361 tgcattgcta gaagttgatc ctgacttccg tttagctctt aaacgtgctg gcttacttac
    18421 tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt aagaaagcac gtaaagcttc
    18481 acaattctca aaacgttaat atcctttata tatcaacgtt tctggcactt ttcaattatt
    18541 tggaaagtgc ttcttttgtt tttaagaaca catttaagaa cacatatcaa tcacacttat
    18601 tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac tatatctggt gtgttcacat
    18661 atatctttgt tgtagaaata tctgagtgtg ttaaagcatt tgaaatttga ttcatatctg
    18721 ctccaccttg tctagctata gttgcataag tatgacggag tttgtgtgga gataaatgaa
    18781 ctaagtcagg atgccttctt ttaattgaat tgattcgata gtttaggtaa tcggcatgaa
    18841 caggacaatt tacttctcca gatggtttag tataagtaaa gagatactga tctaacgttt
    18901 gcttaatttt taatttcaac aattgatcag cttgtaccga tttccattca cttagcaatg
    18961 tcattacaac ttctggtact tgaatgacgg tatcttttcg accttttgta aacttttttc
    19021 tttgatattt atctaaagta tgtttaagac ggaccgtttg tttttcaaaa tcaatatatt
    19081 tccattgcaa agcgtaagtc tcactttttc tatcaccaag atacagggta atcataaaaa
    19141 gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc atcatttact gcttctatcc
    19201 aattagtaag ttcttttgat gatagtgctt gcttaccttc ttgtattctt ttttcttcta
    19261 aagcattttt tttaggtgca gtgatacttt gaataatttt agtagttcga ttaaattcga
    19321 tataatttag tatttctgca atatcgaaca tggaacgaac atatcctttt atgattttta
    19381 tatttgcata ttctttcgat tttttggtta attcatctga tattatctca gtattgatat
    19441 ttagataatt cattgcgtat tcaccaaaca taggtaaaat atgcaaccga aatatatctt
    19501 ttgtattact aatggttata tcactaggag gcctgtttga tctaattgtt tgacctaatt
    19561 catatctagg aagccattta gattcgtaaa attttttaaa cgttattttt ccttttaatt
    19621 ctaatgagtt tgcattctcc tctctcaaaa ctttttctat tttcttcttc atatcatttt
    19681 cggctttaat cgccaattgc ctatttggaa atgttttttg atacttttct gatgcaattc
    19741 ctgttatttc tttaaacctt tttggatatc tcagattagt tttatatttc ccatttgaaa
    19801 gctttgtagg caaaatcctc actccttaaa aaaagtaagg cagtatattt cactaatttt
    19861 agtatatgcg gcgagaagaa aatttcaagt tattttaaat gtattcataa agtctttaat
    19921 atcttctacg tcaaagcgaa ctacaccacc aactcttatt actgggaaat tatatatacg
    19981 aatccactta tctagagtat tattatcgat tcctagaaat gcacaggttt gttttttatt
    20041 caggtagact ctggattcta taaaatcatc tcctaaaata gtagttatga aatttttgaa
    20101 ttgctttggg taagcacata ggaatttcac ctctccccag tttacggcga gccttacgga
    20161 agtatcatta tttgctgcta ttgtcatggc attttgtttt cccagaacaa aaagaaatct
    20221 ctaagtattt gtcgctaatc aggtcatggc gtacttgagt ttctagctcg aatagcaggg
    20281 atcgttattc aaaaattgta tttaattgtc aatgatcaaa aatagttttt ttcctattca
    20341 ctattaataa cgataaacat gtataaaaag acacactacg aaaaaagtta aatggagttt
    20401 actgattttc atatttgtat atggaacaga catagtcttg aataattttt ataaatgttt
    20461 tgatgcttat caacttattc aacccgctat ccacttcaag tataattttt ccatacgcca
    20521 ataaagtagt tttaggagca tgttctcatt tgagaacgct ccttttttat atattttgaa
    20581 agctacttac tatacgcgta gtttcaaata gttttgtcat tctaatcttc agcaacagaa
    20641 aaaaatctaa actataaaat ttagggttga ctaaaatata atttttgtat accatatgat
    20701 cgtgataatc attatcattt agaaggagaa agttatgtta gaagtgaaaa agcttaatgt
    20761 ttgttataac gattttttag cattgaacga gatttcttta aaaatacaag aaggtgcttt
    20821 aacaggtatt attggtccaa atggtgctgg aaaatccaca ttgcttaaag caatgttaaa
    20881 tattatccct catcagggag aggtctccat taataatgaa gatatcaata aaaaactggt
    20941 gaagattgca tatgtagagc aaaaagctga tattgatttt acttttccaa taaaagtaaa
    21001 agaatgtgta tctatgggaa catatgccgg aatgaaagtt tttcaaagaa ttaaaaatgc
    21061 agaatggcaa agagtttcaa aggctttgga aaaggtcgat atgggaaaat attctaatca
    21121 tcaaatagga gaattatctg gaggacaatt tcaacgtgta cttttagcaa gatgtcttgc
    21181 tcaaaatgcg gatttcatat ttttagatga accatttgta gggatagact tagttagtga
    21241 acgaataatt atggatacgt taaaaaaatt gaaacatcag ggaaaaacag tgctaattgt
    21301 gcatcatgat ttaagcaagg taaagaagta ttttgataat attattcttt tgaatcgaaa
    21361 tctaattgct cacggaagtg tagaatctgt atttaatgaa gaaaatttaa aaaaagccta
    21421 tggagatact atatttattg gagaggggga ataatatgct tcaaaatttt attaatggtc
    21481 tgtatgactt tcacttttta cagaatgctc taattacatc tgtagttata gggattgttt
    21541 ctggaacagt aggttgtttt attattttaa gaggaatgtc tcttatgggt gatgctattt
    21601 ctcatgctgt tttgccagga gtagcaattt cttatatttt aggtattaat ttttttattg
    21661 gtgcgattgt
//
LOCUS       DACDCV010000041        21664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDCV010000041 DACDCV010000000
VERSION     DACDCV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.041"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..334)
                     /locus_tag="IV041_001502"
     CDS             complement(<1..334)
                     /locus_tag="IV041_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8382284.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLF"
     gene            418..1359
                     /locus_tag="IV041_001503"
     CDS             418..1359
                     /locus_tag="IV041_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382285.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            1360..2403
                     /locus_tag="IV041_001504"
     CDS             1360..2403
                     /locus_tag="IV041_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382286.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            2409..3479
                     /locus_tag="IV041_001505"
     CDS             2409..3479
                     /locus_tag="IV041_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382287.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            3469..4422
                     /locus_tag="IV041_001506"
     CDS             3469..4422
                     /locus_tag="IV041_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8382288.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            4434..5444
                     /locus_tag="IV041_001507"
     CDS             4434..5444
                     /locus_tag="IV041_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8382289.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5729..6550)
                     /locus_tag="IV041_001508"
     CDS             complement(5729..6550)
                     /locus_tag="IV041_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8382290.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(6647..6925)
                     /locus_tag="IV041_001509"
     CDS             complement(6647..6925)
                     /locus_tag="IV041_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382291.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7193..9202
                     /gene="metG"
                     /locus_tag="IV041_001510"
     CDS             7193..9202
                     /gene="metG"
                     /locus_tag="IV041_001510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8382292.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(9681..10268)
                     /locus_tag="IV041_001511"
     CDS             complement(9681..10268)
                     /locus_tag="IV041_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382293.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            10443..11765
                     /locus_tag="IV041_001512"
     CDS             10443..11765
                     /locus_tag="IV041_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8382294.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            11920..12696
                     /locus_tag="IV041_001513"
     CDS             11920..12696
                     /locus_tag="IV041_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8382295.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            12689..13258
                     /gene="rnmV"
                     /locus_tag="IV041_001514"
     CDS             12689..13258
                     /gene="rnmV"
                     /locus_tag="IV041_001514"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8382296.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            13251..14135
                     /gene="rsmA"
                     /locus_tag="IV041_001515"
     CDS             13251..14135
                     /gene="rsmA"
                     /locus_tag="IV041_001515"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8382297.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            14586..15215
                     /locus_tag="IV041_001516"
     CDS             14586..15215
                     /locus_tag="IV041_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382298.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(15339..16448)
                     /gene="ugpC"
                     /locus_tag="IV041_001517"
     CDS             complement(15339..16448)
                     /gene="ugpC"
                     /locus_tag="IV041_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8382299.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            16657..17079
                     /gene="mgsA"
                     /locus_tag="IV041_001518"
     CDS             16657..17079
                     /gene="mgsA"
                     /locus_tag="IV041_001518"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8382300.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            17402..17938
                     /locus_tag="IV041_001519"
     CDS             17402..17938
                     /locus_tag="IV041_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8382301.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(17978..19741)
                     /locus_tag="IV041_001520"
     CDS             complement(17978..19741)
                     /locus_tag="IV041_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382302.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(19744..21459)
                     /locus_tag="IV041_001521"
     CDS             complement(19744..21459)
                     /locus_tag="IV041_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382303.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         6933 a         3968 c         4388 g         6375 t
ORIGIN      
        1 aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc
       61 tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc tttcggttcc
      121 ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc
      181 ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt
      241 gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa
      301 aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat ttttggttca
      361 ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg
      421 aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta
      481 attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag
      541 gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca
      601 gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc
      661 ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg
      721 cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc
      781 tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt
      841 cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta
      901 caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg
      961 ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta
     1021 ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc
     1081 tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct
     1141 gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg
     1201 gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc
     1261 ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt
     1321 gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt
     1381 tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa
     1441 gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt
     1501 ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc
     1561 tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc
     1621 aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt
     1681 ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt
     1741 ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc
     1801 tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg
     1861 ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc
     1921 ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga
     1981 attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt
     2041 cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt
     2101 gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt
     2161 attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc
     2221 ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag
     2281 acattcctgt atctgcctta tctattatgg attccggcag taactttatc ggttatcatg
     2341 attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag
     2401 tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg
     2461 ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg
     2521 cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc
     2581 tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg
     2641 gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag
     2701 atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta
     2761 gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat
     2821 tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag
     2881 gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg
     2941 ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga
     3001 aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag
     3061 tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa
     3121 catcggaaga aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa
     3181 tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt
     3241 tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg
     3301 atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc
     3361 tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta
     3421 tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa
     3481 ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat
     3541 gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa
     3601 tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat
     3661 ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg
     3721 aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt
     3781 atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct
     3841 gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac
     3901 ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga
     3961 gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg
     4021 tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc
     4081 tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta
     4141 atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt
     4201 catccttata cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga
     4261 aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt
     4321 gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa
     4381 aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa
     4441 aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt
     4501 ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac
     4561 aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct
     4621 taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc
     4681 gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc
     4741 aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt
     4801 taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc
     4861 gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg
     4921 tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga
     4981 cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag
     5041 aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa
     5101 agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt
     5161 atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca
     5221 aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt
     5281 ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata
     5341 aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc
     5401 aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg
     5461 ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa
     5521 tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa
     5581 gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc
     5641 aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg
     5701 attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga
     5761 tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca
     5821 tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa
     5881 atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct
     5941 cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa
     6001 aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt
     6061 ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct
     6121 tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga
     6181 cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt
     6241 cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg
     6301 actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt
     6361 ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga
     6421 tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa
     6481 gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa
     6541 aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga
     6601 ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa
     6661 taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc
     6721 gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca
     6781 aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca
     6841 tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg
     6901 taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt
     6961 tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa
     7021 caaaaacccg catttattca acctattcat ccttttaaag gatagattga acgtagatag
     7081 aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg
     7141 tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga
     7201 aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg
     7261 caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt
     7321 tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga
     7381 agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt
     7441 atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg attaccataa
     7501 agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga
     7561 atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga
     7621 agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt
     7681 agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt
     7741 ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat
     7801 caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg
     7861 gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc
     7921 caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg
     7981 gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc
     8041 aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct
     8101 attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct
     8161 tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg
     8221 gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa
     8281 tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact
     8341 cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc
     8401 taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc
     8461 tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt
     8521 attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga
     8581 atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat
     8641 cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga
     8701 atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat
     8761 ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga
     8821 tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa
     8881 atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt
     8941 agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca
     9001 aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt
     9061 cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat
     9121 cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc
     9181 aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc
     9241 cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat
     9301 catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt
     9361 cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca
     9421 taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct
     9481 tacattcata aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag
     9541 cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat
     9601 gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc
     9661 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
     9721 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
     9781 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
     9841 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
     9901 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
     9961 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
    10021 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
    10081 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
    10141 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
    10201 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
    10261 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
    10321 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
    10381 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
    10441 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
    10501 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
    10561 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
    10621 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
    10681 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
    10741 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
    10801 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
    10861 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
    10921 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
    10981 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
    11041 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
    11101 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
    11161 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
    11221 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
    11281 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
    11341 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
    11401 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
    11461 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
    11521 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
    11581 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
    11641 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
    11701 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
    11761 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
    11821 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
    11881 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
    11941 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
    12001 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
    12061 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
    12121 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
    12181 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
    12241 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
    12301 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
    12361 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
    12421 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
    12481 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
    12541 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
    12601 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
    12661 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
    12721 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
    12781 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
    12841 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
    12901 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
    12961 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
    13021 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
    13081 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
    13141 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
    13201 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
    13261 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
    13321 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
    13381 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
    13441 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
    13501 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
    13561 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
    13621 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
    13681 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
    13741 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
    13801 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
    13861 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
    13921 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
    13981 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
    14041 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
    14101 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
    14161 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
    14221 ttcgtttgtt tgggcttgtt accgcagatg caagcttttg aacaccgttt aagaaagagg
    14281 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
    14341 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
    14401 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
    14461 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
    14521 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
    14581 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
    14641 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
    14701 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
    14761 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
    14821 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
    14881 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
    14941 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
    15001 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
    15061 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
    15121 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
    15181 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
    15241 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
    15301 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
    15361 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
    15421 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
    15481 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
    15541 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
    15601 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
    15661 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
    15721 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
    15781 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
    15841 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
    15901 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
    15961 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
    16021 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
    16081 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
    16141 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
    16201 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
    16261 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
    16321 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
    16381 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
    16441 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
    16501 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
    16561 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
    16621 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
    16681 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
    16741 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
    16801 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
    16861 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
    16921 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
    16981 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
    17041 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
    17101 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
    17161 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
    17221 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
    17281 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
    17341 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
    17401 aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
    17461 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
    17521 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
    17581 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
    17641 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
    17701 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
    17761 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
    17821 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
    17881 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
    17941 aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt
    18001 gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga
    18061 caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat
    18121 gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt
    18181 tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac
    18241 tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt
    18301 gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag
    18361 aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat
    18421 tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac
    18481 gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg
    18541 ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc
    18601 ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct
    18661 gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg
    18721 gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa
    18781 atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga
    18841 taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga
    18901 ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa
    18961 ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc
    19021 ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct
    19081 gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc
    19141 caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca
    19201 caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca
    19261 tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg
    19321 cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt
    19381 gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc
    19441 ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg agccattgac
    19501 tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga
    19561 agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca
    19621 caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat
    19681 aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca
    19741 taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa
    19801 ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc
    19861 ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg
    19921 tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa
    19981 aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc
    20041 gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg
    20101 gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc
    20161 tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg
    20221 aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc
    20281 cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt
    20341 tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt
    20401 aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc
    20461 gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt
    20521 ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac
    20581 gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc
    20641 aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa
    20701 acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt
    20761 ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc
    20821 tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg
    20881 ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat
    20941 gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc
    21001 ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa
    21061 taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt
    21121 gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt
    21181 gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg
    21241 gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc
    21301 aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa
    21361 aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg
    21421 cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat
    21481 ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa
    21541 agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata
    21601 gactattttt tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca ttcataggaa
    21661 cgtc
//
LOCUS       DACDCV010000042        21443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDCV010000042 DACDCV010000000
VERSION     DACDCV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.042"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            124..825
                     /locus_tag="IV041_001522"
     CDS             124..825
                     /locus_tag="IV041_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8382304.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1434..2450)
                     /gene="tsaD"
                     /locus_tag="IV041_001523"
     CDS             complement(1434..2450)
                     /gene="tsaD"
                     /locus_tag="IV041_001523"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8382305.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2447..2911)
                     /gene="rimI"
                     /locus_tag="IV041_001524"
     CDS             complement(2447..2911)
                     /gene="rimI"
                     /locus_tag="IV041_001524"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8382306.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2918..3460)
                     /gene="rimI"
                     /locus_tag="IV041_001525"
     CDS             complement(2918..3460)
                     /gene="rimI"
                     /locus_tag="IV041_001525"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8382307.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3444..4268)
                     /gene="tsaB"
                     /locus_tag="IV041_001526"
     CDS             complement(3444..4268)
                     /gene="tsaB"
                     /locus_tag="IV041_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8382308.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4357..5337)
                     /locus_tag="IV041_001527"
     CDS             complement(4357..5337)
                     /locus_tag="IV041_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8382309.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5361..6845)
                     /gene="galT"
                     /locus_tag="IV041_001528"
     CDS             complement(5361..6845)
                     /gene="galT"
                     /locus_tag="IV041_001528"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8382310.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6857..7846)
                     /gene="galE"
                     /locus_tag="IV041_001529"
     CDS             complement(6857..7846)
                     /gene="galE"
                     /locus_tag="IV041_001529"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8382311.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8094..8261
                     /locus_tag="IV041_001530"
     CDS             8094..8261
                     /locus_tag="IV041_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8382312.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8323..10134)
                     /locus_tag="IV041_001531"
     CDS             complement(8323..10134)
                     /locus_tag="IV041_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8382313.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10131..10496)
                     /locus_tag="IV041_001532"
     CDS             complement(10131..10496)
                     /locus_tag="IV041_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8382314.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10659..11054)
                     /locus_tag="IV041_001533"
     CDS             complement(10659..11054)
                     /locus_tag="IV041_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8382315.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11072..12034)
                     /locus_tag="IV041_001534"
     CDS             complement(11072..12034)
                     /locus_tag="IV041_001534"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8382316.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12034..12246)
                     /locus_tag="IV041_001535"
     CDS             complement(12034..12246)
                     /locus_tag="IV041_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8382317.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12267..12965)
                     /locus_tag="IV041_001536"
     CDS             complement(12267..12965)
                     /locus_tag="IV041_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8382318.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12985..13527)
                     /locus_tag="IV041_001537"
     CDS             complement(12985..13527)
                     /locus_tag="IV041_001537"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8382319.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13659..14663)
                     /locus_tag="IV041_001538"
     CDS             complement(13659..14663)
                     /locus_tag="IV041_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8382320.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14660..15649)
                     /locus_tag="IV041_001539"
     CDS             complement(14660..15649)
                     /locus_tag="IV041_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8382321.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15646..16452)
                     /locus_tag="IV041_001540"
     CDS             complement(15646..16452)
                     /locus_tag="IV041_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382322.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16618..17574
                     /locus_tag="IV041_001541"
     CDS             16618..17574
                     /locus_tag="IV041_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8382323.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17651..18169)
                     /locus_tag="IV041_001542"
     CDS             complement(17651..18169)
                     /locus_tag="IV041_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8382324.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18257..18406)
                     /gene="rpmG"
                     /locus_tag="IV041_001543"
     CDS             complement(18257..18406)
                     /gene="rpmG"
                     /locus_tag="IV041_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8382325.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18634..19080
                     /locus_tag="IV041_001544"
     CDS             18634..19080
                     /locus_tag="IV041_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8382326.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19275..21170)
                     /gene="asnB"
                     /locus_tag="IV041_001545"
     CDS             complement(19275..21170)
                     /gene="asnB"
                     /locus_tag="IV041_001545"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8382327.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6453 a         4550 c         3699 g         6741 t
ORIGIN      
        1 ataataacaa attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa
       61 ctttacaaat tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt
      121 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
      181 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
      241 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
      301 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
      361 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
      421 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
      481 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
      541 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
      601 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
      661 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
      721 gctggacaag taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt
      781 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
      841 gcagtgaccc tgataaatat cagggtcact gcttattttc ctccctaaaa cttctgaaag
      901 atccggcagt tttagatcac tggacaagac tcaaagattt tctatgtgtg tttcagttat
      961 gtaatattca ctgtttgcaa aaaatactgt tagattaact agatgaagcg tcttgtcctc
     1021 atccgatcta gattattcga tattcctcga caataaacga ttactcagtg agggcaatca
     1081 gaggaacgga taatgataaa taagctgtaa aaaatagcaa aataacaata atgattaaaa
     1141 gtatgtaaaa aatagggctt tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg
     1201 tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt
     1261 tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg
     1321 cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt
     1381 ttttcctggg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctttattca
     1441 gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt
     1501 tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga
     1561 atcactagtt ctacctcagg gagtttagct gccaaagcat cctgcaatcc ttcacgcaac
     1621 ccttgattgg ccgctacacc tccagctata atcagttgct tgactggata attttgacaa
     1681 gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa
     1741 tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca
     1801 ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa
     1861 tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa
     1921 agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc
     1981 ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta
     2041 acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc
     2101 cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct
     2161 gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc
     2221 gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt
     2281 tgttctacgt gatggcggct agctacttca ggtactacgc caccaaaacg tttatgactg
     2341 tttatttgag aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca
     2401 ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct
     2461 ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt
     2521 tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct gcttcattag
     2581 atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt
     2641 gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga
     2701 tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcag
     2761 ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc
     2821 aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg
     2881 cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta
     2941 tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt
     3001 gaaaccccaa tttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg
     3061 acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac
     3121 cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac
     3181 cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata
     3241 gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc
     3301 cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc
     3361 ttagtacgta acgacgatcg atttgttcga ctgttttttc tgtataaatc attttatctt
     3421 gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg
     3481 tgcgtctcca accatttttc ttctgcttct actcgtttta aatagcgagg aagaaaagct
     3541 tgtttctctg caactggttc aactaaagca cctaattgag ccacagttac accacttgga
     3601 atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt tatttcagag
     3661 aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt
     3721 gtgaacaaat cagaagcaga aatatgtaca tcttcaagta cagtttctaa tctattttct
     3781 ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata
     3841 atattatcca caccgatgca attagccgcg atggttttca gactagatac tcctactagc
     3901 tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta
     3961 taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga
     4021 tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt
     4081 gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt
     4141 gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct
     4201 tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa
     4261 taatacatga ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg
     4321 cggtatcact ccattttccc acagcttttc catcttttat ttcgttgatt ctcgaataat
     4381 caactctgtt tccacaataa tctttcttga aaccggtgct ttggaattac ttaattgaag
     4441 aatcgtctcc actgctagct ctcccatcca ttcggtataa acttttacag tagaaagcga
     4501 gggcgttaca tatttcgcta cactgacatc attgaagccc atcacagaaa tatcttctgg
     4561 taccactaaa cctttttctt gaatcgcttt cagtgcgcca atcgctaatg catcattagc
     4621 tgcaaaaagc gcatcaggaa catgcggatt cgtttttaaa tattgcttca ctgcttgata
     4681 tcctgactcg acagaaaaat ctgcttcgat aattggacct tcgtcgtagc ctttttctgc
     4741 caaaacttgt tgaaatgcca taattctggg atcttttagc cgctgatggt ttttcttcgt
     4801 atattctgtt ccagcaagca tagcgatatg ttgcgcttta tgggagacaa agtgttcaat
     4861 gactttttga acacttgact taaagtcgac aactagagag tcgattcctt gagaagtgcc
     4921 gtcagaatct accaaaagca aggcattttg ggatcctttc atttctaata actgagcttc
     4981 gtcgaatttt cctagtgcga tcgttccatc tgccggaaga tcactaatgg aatcccacgt
     5041 ttctttcaat aaagtaacgt ttgcttcttc tgcctttttt tcgattccta ggcgaataga
     5101 taaataataa agatcttcaa gctcttccgt atcgtcgtac cactggatca ggcgaagcgt
     5161 ttgcttagta tttgctgcat ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc
     5221 aaaaattttt tgctttgtgg ctaagccaac ggaaagatca ggatcatagt tgagaactct
     5281 tgaaacagta gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg
     5341 tctctcctcc ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct
     5401 ttttcatctc gcttgaatac tccagcgtct tccagtacac gtgcaaaaac ctgacctaca
     5461 gatttttgta cgacatcggt gaccgtatct ttcgtcaaat caggatgttc tttttttaat
     5521 tgttctgccc acggacgatg atattcagcc atctggtttt cttctcctaa taagaaatgc
     5581 tctacttcta ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc cattacttcg
     5641 atcaacccaa tgttctcctt tttgatatgt tggacatctt tatgtggatg aaaaatccca
     5701 tcaggatgtt cttctgaaac attgttgtct cgaaggacaa ggtccaactc aaagtaattg
     5761 tcccttcttc gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct
     5821 ctgatagata ctgtttcatc cgaatatgct ttccattttt ccaatactga gttagctgct
     5881 tcaataagca agtttttctc tgttgattgc aaacggatca cagacatcgg ccatttcacg
     5941 actcctgcga tgacttgcgg ataatcttct agtttgataa gtttttctat cggtgctttt
     6001 gccataggaa attcatgacg tcctgcctga taatgatcat gagaaagaat cgatcctcca
     6061 acaattggta agtcagcatt cgaaccaaca aagtaatgag gcaagacttc agtaatccga
     6121 agcagtctag caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg
     6181 atcgaatgtt cattgtaata agcataaggg gaatattgga atccccagct ttcaccgtct
     6241 agattcattc gaataatccg gtgattggtg cgggctggat agttcaagcg acctctatat
     6301 ccttcgtttt ccatgcaaag catgcaggta ggatagtcta cttttaccgc ctcgcgttct
     6361 gctgcgattt gttttggatc tttttctggc ttagataaat tgattgtgat ttccagttca
     6421 ccatactccg actctgccgg aaatttgatg ttttttgcga tggctcttgt tttgatgtag
     6481 tcatttttct tacttaaatt aaagaaatac tcagttgctt cctctgggct tttcgaatag
     6541 tgctgtgcaa agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc cattaattgc
     6601 gcttcaaaaa tctcttgttc tgctggcaat tctgaaagaa caccattttc tcttgcctta
     6661 gcgacaagct gatccaataa gtctacagat tcttccttaa ctggtcttac ttcaatgctt
     6721 tctagagcat cttctcctac catccctaaa agacgattct gtaaatataa gcggtccatt
     6781 tccatccatc cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt
     6841 gacatatctc attcccctat tcttcataac catttggatg gctgacatgc caatcccatg
     6901 ctgttttgat aatcgctttg atatctgtgt attcaggttt ccagcccaga atctctctcg
     6961 ctttttcact tgaagcgact aatcggctag gatctccagc tcttcttggt gctatttctg
     7021 ctggaatttc ttttcctgtc acttctctag ctgcttctag catttccttg actgagtatc
     7081 ctttgctgct gcctaaattg aagaagttgc tttcatttcc ttctttcaag tattccaaag
     7141 ctaaaatatg tgcagcaatc aaatcttcta cttgaacata atctctgata catgttccat
     7201 ctggtgtatc ataatcgtct ccataaacag ccaaggcttt tctttgacca agagctactt
     7261 gtaaaatgat tgggacaaga tgcgtttctg gtgtatgatc ttctccgata gatgcatctg
     7321 cttttgctcc ggctacattg aagtagcgca aagcgacata gcgcatgcca tatgcttggt
     7381 cacaccactt catcattttt tccatcatca atttactttc accatacgga tttttaggat
     7441 tagctggtgt atcttctgtg attggagact ctttcggctc tccataagtt gctgcagtag
     7501 aagagaatac gattttattc acgttgaact cgtgcatgac ttcaagtaag atttgcatac
     7561 cgtatacgtt gttgttgaaa tacatcaatg gtttttcgac agattctcct actaaagagc
     7621 ttgcagcaaa atggattact ccttcaatcg gttccttttc aaatacggaa cgtagaaatt
     7681 ctttatcccg gatgtctcct tcataaaaat gagcatctgg atgtactgct tgtttgtgac
     7741 ctgtgagcaa attatctacc acagctactt gatacccctt ttgaactagc tggtctacag
     7801 cgtgcgaacc gatataaccg gctccgccta gtactaaaat tgtcataaaa aacactcctt
     7861 tgattagtaa aattgtgttt gttgattcta tataatttaa aaaacgaagt ttacttttat
     7921 ggtgaaaaca aacaaagtaa aatttcgtaa attcatatgg taaatttata ataaaacatt
     7981 tagtaaacat tttcaagggt tttttgcaaa ggtttacaaa caaacgtaaa actagccttt
     8041 ttttctcttt tgttccataa tagatataac aaataataaa tgaggtggat tcaatgaaaa
     8101 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
     8161 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
     8221 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
     8281 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
     8341 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
     8401 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
     8461 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
     8521 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
     8581 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
     8641 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
     8701 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
     8761 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
     8821 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
     8881 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
     8941 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
     9001 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
     9061 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     9121 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     9181 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     9241 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     9301 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     9361 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     9421 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     9481 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     9541 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     9601 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     9661 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     9721 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     9781 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     9841 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
     9901 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
     9961 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
    10021 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
    10081 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
    10141 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
    10201 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
    10261 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
    10321 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
    10381 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
    10441 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
    10501 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
    10561 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
    10621 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
    10681 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
    10741 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
    10801 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
    10861 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
    10921 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
    10981 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
    11041 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
    11101 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
    11161 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
    11221 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
    11281 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
    11341 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
    11401 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
    11461 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
    11521 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
    11581 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
    11641 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
    11701 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
    11761 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
    11821 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
    11881 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
    11941 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
    12001 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
    12061 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
    12121 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
    12181 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
    12241 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
    12301 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
    12361 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
    12421 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
    12481 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
    12541 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
    12601 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
    12661 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
    12721 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
    12781 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
    12841 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
    12901 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
    12961 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
    13021 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
    13081 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
    13141 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
    13201 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
    13261 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
    13321 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
    13381 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
    13441 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
    13501 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
    13561 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
    13621 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
    13681 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
    13741 gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact aagcagaaaa
    13801 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
    13861 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
    13921 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
    13981 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
    14041 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
    14101 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
    14161 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
    14221 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
    14281 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
    14341 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
    14401 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
    14461 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
    14521 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
    14581 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
    14641 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
    14701 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
    14761 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
    14821 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
    14881 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
    14941 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
    15001 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
    15061 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
    15121 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
    15181 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
    15241 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
    15301 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
    15361 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcacccct tgcatcaatg
    15421 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
    15481 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
    15541 aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
    15601 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
    15661 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
    15721 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
    15781 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
    15841 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
    15901 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
    15961 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
    16021 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
    16081 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
    16141 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
    16201 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
    16261 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
    16321 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
    16381 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
    16441 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
    16501 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
    16561 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
    16621 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
    16681 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
    16741 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
    16801 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
    16861 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
    16921 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
    16981 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
    17041 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
    17101 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
    17161 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
    17221 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
    17281 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
    17341 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
    17401 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
    17461 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
    17521 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
    17581 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
    17641 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
    17701 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
    17761 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
    17821 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
    17881 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
    17941 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
    18001 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
    18061 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    18121 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    18181 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    18241 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    18301 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    18361 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    18421 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    18481 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    18541 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    18601 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    18661 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    18721 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    18781 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    18841 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    18901 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    18961 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    19021 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    19081 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    19141 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    19201 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    19261 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    19321 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    19381 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    19441 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    19501 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    19561 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    19621 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    19681 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    19741 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    19801 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    19861 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    19921 ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt tccgatttta
    19981 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    20041 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    20101 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    20161 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    20221 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    20281 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    20341 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    20401 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    20461 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    20521 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    20581 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    20641 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    20701 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    20761 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    20821 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    20881 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    20941 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    21001 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    21061 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    21121 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    21181 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    21241 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    21301 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    21361 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    21421 aagacacgac ctaaagatcg tgt
//
LOCUS       DACDCV010000043        20991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDCV010000043 DACDCV010000000
VERSION     DACDCV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.043"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..496
                     /gene="rpsJ"
                     /locus_tag="IV041_001546"
     CDS             188..496
                     /gene="rpsJ"
                     /locus_tag="IV041_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8382328.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            527..1156
                     /gene="rplC"
                     /locus_tag="IV041_001547"
     CDS             527..1156
                     /gene="rplC"
                     /locus_tag="IV041_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8382329.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1184..1807
                     /gene="rplD"
                     /locus_tag="IV041_001548"
     CDS             1184..1807
                     /gene="rplD"
                     /locus_tag="IV041_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8382330.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1807..2097
                     /gene="rplW"
                     /locus_tag="IV041_001549"
     CDS             1807..2097
                     /gene="rplW"
                     /locus_tag="IV041_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8382331.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2136..2969
                     /gene="rplB"
                     /locus_tag="IV041_001550"
     CDS             2136..2969
                     /gene="rplB"
                     /locus_tag="IV041_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8382332.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3012..3290
                     /gene="rpsS"
                     /locus_tag="IV041_001551"
     CDS             3012..3290
                     /gene="rpsS"
                     /locus_tag="IV041_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8382333.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3313..3660
                     /gene="rplV"
                     /locus_tag="IV041_001552"
     CDS             3313..3660
                     /gene="rplV"
                     /locus_tag="IV041_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8382334.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3674..4330
                     /gene="rpsC"
                     /locus_tag="IV041_001553"
     CDS             3674..4330
                     /gene="rpsC"
                     /locus_tag="IV041_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8382335.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4333..4767
                     /gene="rplP"
                     /locus_tag="IV041_001554"
     CDS             4333..4767
                     /gene="rplP"
                     /locus_tag="IV041_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8382336.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4757..4945
                     /gene="rpmC"
                     /locus_tag="IV041_001555"
     CDS             4757..4945
                     /gene="rpmC"
                     /locus_tag="IV041_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8382337.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4970..5236
                     /gene="rpsQ"
                     /locus_tag="IV041_001556"
     CDS             4970..5236
                     /gene="rpsQ"
                     /locus_tag="IV041_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8382338.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5294..5662
                     /gene="rplN"
                     /locus_tag="IV041_001557"
     CDS             5294..5662
                     /gene="rplN"
                     /locus_tag="IV041_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8382339.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5699..6007
                     /gene="rplX"
                     /locus_tag="IV041_001558"
     CDS             5699..6007
                     /gene="rplX"
                     /locus_tag="IV041_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8382340.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6035..6574
                     /gene="rplE"
                     /locus_tag="IV041_001559"
     CDS             6035..6574
                     /gene="rplE"
                     /locus_tag="IV041_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8382341.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6593..6778
                     /locus_tag="IV041_001560"
     CDS             6593..6778
                     /locus_tag="IV041_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8382342.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6814..7212
                     /gene="rpsH"
                     /locus_tag="IV041_001561"
     CDS             6814..7212
                     /gene="rpsH"
                     /locus_tag="IV041_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8382343.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7244..7780
                     /gene="rplF"
                     /locus_tag="IV041_001562"
     CDS             7244..7780
                     /gene="rplF"
                     /locus_tag="IV041_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8382344.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7941..8297
                     /gene="rplR"
                     /locus_tag="IV041_001563"
     CDS             7941..8297
                     /gene="rplR"
                     /locus_tag="IV041_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8382345.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8318..8818
                     /gene="rpsE"
                     /locus_tag="IV041_001564"
     CDS             8318..8818
                     /gene="rpsE"
                     /locus_tag="IV041_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8382346.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8833..9012
                     /gene="rpmD"
                     /locus_tag="IV041_001565"
     CDS             8833..9012
                     /gene="rpmD"
                     /locus_tag="IV041_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8382347.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9056..9496
                     /gene="rplO"
                     /locus_tag="IV041_001566"
     CDS             9056..9496
                     /gene="rplO"
                     /locus_tag="IV041_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8382348.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9496..10791
                     /gene="secY"
                     /locus_tag="IV041_001567"
     CDS             9496..10791
                     /gene="secY"
                     /locus_tag="IV041_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8382349.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10853..11500
                     /locus_tag="IV041_001568"
     CDS             10853..11500
                     /locus_tag="IV041_001568"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8382350.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11691..11909
                     /gene="infA"
                     /locus_tag="IV041_001569"
     CDS             11691..11909
                     /gene="infA"
                     /locus_tag="IV041_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8382351.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11942..12058
                     /gene="rpmJ"
                     /locus_tag="IV041_001570"
     CDS             11942..12058
                     /gene="rpmJ"
                     /locus_tag="IV041_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8382352.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12076..12441
                     /gene="rpsM"
                     /locus_tag="IV041_001571"
     CDS             12076..12441
                     /gene="rpsM"
                     /locus_tag="IV041_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8382353.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12469..12858
                     /gene="rpsK"
                     /locus_tag="IV041_001572"
     CDS             12469..12858
                     /gene="rpsK"
                     /locus_tag="IV041_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8382354.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12938..13876
                     /locus_tag="IV041_001573"
     CDS             12938..13876
                     /locus_tag="IV041_001573"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8382355.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13904..14284
                     /gene="rplQ"
                     /locus_tag="IV041_001574"
     CDS             13904..14284
                     /gene="rplQ"
                     /locus_tag="IV041_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8382356.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14556..15395
                     /locus_tag="IV041_001575"
     CDS             14556..15395
                     /locus_tag="IV041_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8382357.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15371..16240
                     /locus_tag="IV041_001576"
     CDS             15371..16240
                     /locus_tag="IV041_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8382358.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16230..17027
                     /locus_tag="IV041_001577"
     CDS             16230..17027
                     /locus_tag="IV041_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8382359.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17230..17976
                     /gene="truA"
                     /locus_tag="IV041_001578"
     CDS             17230..17976
                     /gene="truA"
                     /locus_tag="IV041_001578"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8382360.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18309..20978)
                     /locus_tag="IV041_001579"
     CDS             complement(18309..20978)
                     /locus_tag="IV041_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8382361.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         6967 a         3845 c         4537 g         5642 t
ORIGIN      
        1 agtttatata tattataatg acaaaggtgc gtttacttta gtgaattgca cagacgtaat
       61 cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg ctttgccacg
      121 aacgagcgtg acggaaattt tcgtggagct atgtctactt ttcaaaatag gcgaaggagg
      181 gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt
      241 agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg
      301 tccaattcca ttaccaactg aacgcagtct ttacacaatc atccgtgcga ctcataaata
      361 caaagattca cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa
      421 cccaacacca aaaactgttg atgctttgat gaagctagac ttaccatctg gtgtaaacat
      481 cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt gtactcatga ccaaaggaat
      541 cttagggaaa aaagtgggaa tgacacaaat cttcactgaa tctggtgaat taattccagt
      601 aactgttgta gaagctactc caaacgtagt actacaagtg aaaacaatgg aaacggatgg
      661 ctacgaagct attcaagttg gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc
      721 gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa
      781 tgttgagcta ggagaatatg aagtaggaaa agaaattaca gtagatgttt tccaagcagg
      841 agacattatt gatgtaacag gtactacgaa aggtaaagga ttccaagggg ttatcaaacg
      901 ccacggacaa agccgcggac caatggctca cggttctcgt taccatcgtc gtcctgggtc
      961 aatgggtcca gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg
     1021 tggcaaccgc gtaactatcc aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt
     1081 tatcttgatc aaaggtaatg ttcctggagc taaaaaatct ttaattacta tcaaatcagc
     1141 tgtgaaagct aaataattgt aggaagaaag gaggaactaa ggaatgccga atgtagcatt
     1201 attcaaacaa gatggaagcc aaaacggtga aatcacactt aacgaagaaa tcttcggaat
     1261 tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt cattaagaca
     1321 aggaacacac gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta aaccatggcg
     1381 tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc tcaccacaat ggcgtggagg
     1441 tggcgtagtc tttggaccaa caccacgttc ttacagctac aaacttccta aaaaagttcg
     1501 tcgtttagca atgaaatctg tattgtcaga aaaagttgct gaaaacaact tggtagcaat
     1561 cgaaggcttg aacttcgacg caccaaaaac aaaagaattc aaacaagttc ttgctaactt
     1621 atctatcgac tctaaagtat tggttgttct agaaaaaggt aacgactttg ctgctttatc
     1681 agcacgtaac ttaccaaacg tttctgtagt aacttctgat aatgtgagtg tgctagacgt
     1741 tgtatcaaat acaaaagtgt tagcaacaca aactgctctt actcaaattg aggaggtgct
     1801 tgcataatga acttactaga cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt
     1861 gccatggatg acaagaaata cacttttgaa gtggacactc gcgcaaacaa aactttagta
     1921 aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta
     1981 cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg
     2041 atcgttacat tgactgaaga ttcaaaagaa atccaattat tcgaagctgc tgaataagca
     2101 ctaatagata actcattaaa ttaggaggga aaaacgtggc gattaaaaag tacaaaccta
     2161 cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca acatcaacac
     2221 ctgaaaaatc attgttacaa ccattgaaaa accatgctgg acgtaacaac aacggccgca
     2281 tcactgtacg tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc gacttcaaac
     2341 gtaacaaaga taacgttgtt gcaactgttc aaacgatcga atatgatcca aaccgttcag
     2401 ctaacatcgc gttagtacac tatgaagatg gagtgaaagc ttacatctta gcacctaaag
     2461 gactagaagt gggtatgaaa cttgtttcag gtccagaagc agatatcaaa gtcggaaatg
     2521 ctcttccatt agaaaacatt ccagttggta ctgttatcca caacattgaa atgaaacctg
     2581 gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag
     2641 gcaaatacgt attgatccgc ttgaactctg gtgaagttcg tatgatctta gctgcatgcc
     2701 gtgctacaat cggttctgta ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg
     2761 gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg
     2821 atcacccaca cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc
     2881 cttggggtca accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac
     2941 ttattgttcg tcgtcgcaaa aataaataat tactttgacc atgtgtcgca cattttgaga
     3001 ggaggttcac catgggtcgt agtttaaaga aaggaccttt tgtcgatgag catttaatga
     3061 aaaaagttga agctcaagca ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc
     3121 gttcaacagt tttcccaagt tttgttggat ataccatcgc agtttatgat ggacgtaaac
     3181 atgtaccagt ttacatccaa gaagacatgg taggacacaa attaggtgaa tttgcaccta
     3241 caagaactta tcgtggacat gctgctgacg ataagaaaac gaaacgctaa tttgagggga
     3301 ggataaatcc aaatggcaga acaaatcaca tcagctaaag caactgcaaa aacagttcgc
     3361 acttcacctc gaaaagcccg tttagtaatt gaccttatca gaggtaaaag tgtcgcagat
     3421 gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg gaatcattga aaaagtattg
     3481 atgtcagcag ttgcgaatgc agaaaataac tttgacttag atgttgaaaa cttggtagta
     3541 agcgaagctt ttgttaacga aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga
     3601 tcagcttctc caatcaacaa acgtacaagt catatcacgg tagtcgtatc agaaaaataa
     3661 ggagggataa tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag gcatcatccg
     3721 cgactgggat gctaaatggt atgctgaaaa agagtatgct gagttcttac acgaagattt
     3781 aagaatccgt aaatttattt caactaaact tgctgatgct gctgtatcaa cagttgaaat
     3841 cgaacgcgca acaggtcgcg taaacatttc aattcacaca gctaaaccag gtatggttat
     3901 tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa
     3961 aagaatccat atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa aattagtagg
     4021 tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc
     4081 catccaacgc tcaatgcgtg ctggcgctca agggatcaaa actcaagtat ctggtcgtct
     4141 aaacggagca gatatcgctc gttcagaagg atactcagaa ggaactgttc ctcttcatac
     4201 tttacgtgcg gacattgatt acgcatggga agaagcagat acaacatacg gaaaactagg
     4261 agttaaagtg tggatctatc gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg
     4321 agggaaataa tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa
     4381 atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct
     4441 gttgattcac attggatcac aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt
     4501 tacatgaaac gtggtgggaa agtatggatt aaaattttcc ctcacaaatc ttatactgcc
     4561 aaagcaattg gggtacgtat gggttctggt aaaggggcac ctgaaggatg ggttgcacca
     4621 gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa
     4681 gcgttacgtc tagcttctca caaattacca atgaaaacta agatcgtaaa acgtgaggaa
     4741 atgggtggtg aatcgaatga aggttaaaga aatcagagaa ttaaccactg ccgaaatgct
     4801 tgatcaagaa aaacaattaa aagaagaatt gtttaatctt agattccaat tagcaacagg
     4861 tcaattagaa aacactgcac gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac
     4921 tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc attaagcgta tgactgaagg
     4981 aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat
     5041 taccgttgct gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca tgaactattc
     5101 taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat
     5161 tatggaaact cgtccattat cagctacaaa acgtttccgt ttgttggaag tagtcgaaga
     5221 agcagttatt atctaataac acgagatttt cctatataga ttgaatgcaa aatctgaaag
     5281 gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc
     5341 acgtgaaatt ttgacgatca aagtccttgg tggatctggt cgtaaaactg ctaacatcgg
     5401 tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga
     5461 cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag acggttcata
     5521 tattaaattt gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac
     5581 acgtatcttt gggccagttg cacgtgaact acgtgaaaac aacttcatga agatcgtttc
     5641 actagcacca gaagttttat aatcttgaga cacttatcaa aggaggtgcg aaacagttat
     5701 gtttgttaaa aaaggcgata aagttaaagt tatcactgga aaagacaaaa acaaagaagg
     5761 cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt
     5821 taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg agatggaagc
     5881 gccaattcat gtttctaacg tgatggtggt tgactctact ggtgtagctg gccgagttgg
     5941 ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga
     6001 taaataaaag tttaaggaag gagggtctta ctgaatgaac cgcctaaaag aaaaatatct
     6061 taaagaagta actccatcat tgatggaaaa atttaactat agctctgtta tgcaaacacc
     6121 taaagttgaa aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa
     6181 cttagataaa gcagttgaag aattaacttt gatcactggt caaaaaccaa tgattacaaa
     6241 agctaaaaaa tcaatcgctg gattccgttt acgtgaagga atgccaattg gtgcaaaagt
     6301 tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg tttcactacc
     6361 acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac
     6421 tttaggtatt aaagaacaat tgatcttccc agaagtagat tatgatttag tagataaagt
     6481 acgtggtatg gacatcgtta ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt
     6541 gttgacacaa ttaggcatgc cattccaaaa ataattaaag gaggcgaact acgtggctaa
     6601 aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag cttatactcg
     6661 ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag ttccatcttt gccgtatttg
     6721 cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa
     6781 agtaaattcg cataagaagg aggtaaaaga tcaatggtca tgacagatcc aattgcagat
     6841 tttctaactc gtatccgtaa cgcaaacatg gttaaacatg agtctttaga agtacctgca
     6901 tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt
     6961 gaatacatcg aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac
     7021 ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac caggtttgcg tgcttatgtc
     7081 aaagctgacg aagtaccaaa agtattgaac ggtttaggta tcgcaatcat ttcgacttct
     7141 gaaggtgttg tcactgataa agaagctcgt gaaaaaaata tcggcggcga agttatcgct
     7201 tacgtatggt aatcaagtag aatctacaag gaggtgtctt taagtgagcc gtatcggaaa
     7261 taaagtcgtt gttatccctg aaggagtaac agttactcaa gacggaaaca acgttacagt
     7321 taaaggacca aaaggtgaat tgactcgtac tttttcagca gatatcaaaa tgaatatcga
     7381 aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg
     7441 aacaactcgt gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc
     7501 gttagaacta atcggggttg ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa
     7561 cgttggttac tcacatccag tagaaatcac accaccagct ggagtaactg ttgaagtacc
     7621 agctaacaca caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc
     7681 aaacattcgt ggaactcgtc ctccagaacc ttataaaggt aaaggtattc gttatgttgg
     7741 cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa aaattgcata
     7801 gaagccagca gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa atacattttt
     7861 acttgtgccg cagcttctat ttttcgaaac gtatttactg cttgaaatag tagtacaata
     7921 aaaataaaga ggtgacaatt gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga
     7981 gacatcgtcg tgtacgtaac aatatctctg gtactgctga gcgcccacgc ttgaacgttt
     8041 ttcgttctaa caaaaacatc tacgctcaaa ttattgatga cgtagcgggt gtaacgctag
     8101 caagtgcctc taccttggat aaagaaattt caggtggaac aaaaacagaa gcagcacaag
     8161 ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg
     8221 accgtggtgg atacctttac catggccgtg tgcaagcttt agctgaagct gctcgcgaaa
     8281 atggactaga attttagaag aaggaggaat accattcatg gtttatatcg atccaaaaca
     8341 tttggaatta gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg
     8401 acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac aaaaacggac acgtagggtt
     8461 tggtacaggt aaagctcaag aagttcctga agcaatccgt aaagcaattg aagatgcgaa
     8521 gaaaaactta gttgaagtac ctatggttgg ttcaacaatc ccacacgaag taatcggtgc
     8581 ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg
     8641 tggaccagtt cgtgccgtat tagaattagc tggggtagca gatatcactt ctaaatcttt
     8701 aggctcaaat acaccaatca acgttgttcg cgcaacagtt gaaggtttga aacaattgaa
     8761 acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt gaagaattaa tcggataagg
     8821 aggacaacaa taatggctga attaaaagtt actttaaaac gcagtattat cggacgtcct
     8881 caaaaccaac gcgatacagt taaagcgtta ggtcttacaa agattaacag ttcagtggtt
     8941 aagccttcta atgaagcaat taaaggcatg atcaatactg tttctcacct agtggacgtt
     9001 gaagaaattt aatttagaaa aatcataact agaacattaa ggaggtgcca aacctatgaa
     9061 acttcatgaa ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg
     9121 tacttcatct ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa aagctcgttc
     9181 aggcggcggt gtgcgtttag gatttgaagg gggacaaaca ccattgttcc gtcgtttacc
     9241 aaaacgagga ttcacaaacg tgaaccgtaa agactatgca gtcattaatc ttgatgtatt
     9301 gaaccgcttt gaagacggag ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt
     9361 gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg gaactaaaca agaaattaac
     9421 tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca attgaagcgg ctggtggatc
     9481 aatcgaggtg atctaatgtt tagactatta aaagatgctt ttaaagtaaa agacattaga
     9541 tcgaaaatcc tatttactgt atttgtgttg tttgtatttc gcctaggtgc acatattact
     9601 gtaccaggcg taaatgctgc ttcattgcaa aacctttctt ctttgccatt tttcaacatg
     9661 ttgaacttgg taagtggtag tgcgatgcaa aacttttcca ttttctcaat gggggtttca
     9721 ccatacatta ctgcttctat cgtcattcag cttcttcaaa tggacatcgt gcctcgattt
     9781 gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat
     9841 ctgacattag tattggcatt tatccaatct gtcggtatca ctgctggatt caatcaatac
     9901 gctcaattag gtttcgtgaa taatcctaac gtagcaactt acgtaacgat cggtttgatt
     9961 ctaacagccg gtacgatgtt tgttacttgg ctaggagaac agatcacaga aaaaggtatc
    10021 ggaaatggcg tatcaatgat catttttgca ggtatcattt ctagattgcc tgaaagtatc
    10081 agagaaattt atgaagatta ctttataaat attgatcctt cagacatatg gaaatctgct
    10141 atttttgtgg ccattttact agtagcggtc ttgttgatcg tgacattcgt aacatttttc
    10201 caacaagctg aacgcaagat cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca
    10261 agcagctatc tgccgttgaa agtaaatgca gctggagtta ttccagttat ctttgctagt
    10321 tcgtttattg ctacgccaaa tgctgtttta caagcattga gcggtagatt cggttctgaa
    10381 aactggtaca aagtgatgat ggagatcttt aactataata cagcacctgg tgctacgatt
    10441 tatacgattc tgatcgttgc cttcactttc ttctacgcct ttgttcaagt aaaccctgag
    10501 aaattagcgg aaaacttgca aaagcaagga agttatatac caagcgtacg ccctggtaag
    10561 gggactgaag agtatgtctc aagattactg atgcgtttaa gtacagttgg tgcagtcttc
    10621 cttggtttag tggcattgct tccaatcatt gctcaaatgg tttgggatct tccacaatca
    10681 atcggattag gcggtacaag cttactgatc gttatcggtg tagctttaga aactgcgaaa
    10741 cagttagaag gcttaatgct gaaacgtaaa tatgttggat tcattgagta agaaagcgtg
    10801 ttgaggtttc ccctcaacac acgttctcac acatttatta ggagggaaaa caatgaacct
    10861 cattttaatg gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc
    10921 ttatggtatt ccgcacatct caacaggaga tatgttccgc gcagcgatgc aaaacgaaac
    10981 agctcttggc ctggaagcaa agtcttatat ggataaaggt gcattagttc ctgatgaagt
    11041 aacaaatgga atcgtgaaag agcgactagc tgaaccagat acagagaaag gcttcttatt
    11101 agatggcttt ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc taaaagattt
    11161 gaataaaaaa attgatgctg tcatcgatat ccatgttggt gaagaaatct tggtcgaacg
    11221 tctagccggc cgatttatct gcagcaattg tggagcaact taccataaaa ttttcaaccc
    11281 tacaaaagtt gaagacacat gtgatcgttg cggtgggcat gaattctatc aaagagaaga
    11341 tgataaacct gagacggtta aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat
    11401 cttggcttat tataaagaac aaggtttgct aaacactatt gatggtgctc gtgaaatcga
    11461 tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac
    11521 ttgcattatg cagttactct tgtcaagttc aataaattat gatagaatat gtcagtccga
    11581 cttctagaga atcctctaga tgatccattt tgagaatttt gaggtgggaa ctgcagtttc
    11641 cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag
    11701 aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag
    11761 ttgaactaga aaacggacac caagttcttg ctactgtttc aggtaaaatt cgtatgcatt
    11821 acatccgtat cttacctggt gacaaagtaa cggttgaatt gtctccatac gatcttaacc
    11881 gtggccgtat tacttatcgc tttaaataat tgtactccgt aaaactacag gaggtattat
    11941 catgaaagta agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa
    12001 aggacgcgtt atggtgattt gcccagcaaa tccaaaacat aaacaacgtc aaggataatt
    12061 ggaggtgcta taaatatggc tcgtattgca ggagtagata ttcctcgtga taaacgtgta
    12121 gtagtttcac ttacttatat ctatggtatt ggtaacacta ctgctaaaaa gatcttagct
    12181 gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct
    12241 atccgtgcag aagtagataa attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg
    12301 aacatcaaac gtttgatgga aatcggttct taccgtggaa tccgtcatcg ccgtggattg
    12361 cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct
    12421 atcgctggca agaaaaaata attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa
    12481 aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag caggtattgc acatatccat
    12541 tctacattca ataacactat catcatgatc actgatactc acggaaatgc attagcgtgg
    12601 tcatcagctg gttcattagg tttcaaagga agcaaaaaat caactccttt tgccgctcaa
    12661 atggcagcag aaactgctac aaaagcagca atggaacacg gattaaagac tgttgaagta
    12721 acagttaaag gacctggttc tggacgtgaa gcagcaattc gttcattaca agctacaggt
    12781 ttagaagtga ctgcaattcg tgacgtgact ccagttcctc ataatggtgc ccgccctcca
    12841 aaacgccgtc gtgtttaatg agtgccgctc attttgagtt ttatcagtaa cgtcattgaa
    12901 caagacactt ttcgttttga aaggggtaaa gataagaatg attgagttcg aaaaaccaag
    12961 aatcgcaaaa attgatgaag aaaaagatta tggcaagttc atcgttgaac ctcttgaaag
    13021 agggtatggg actactttag ggaattccct acgtcgtatt ttattatctt ctttgccagg
    13081 tgctgccatc actaatattc aaatcgatgg tgtactgcat gaattctcta ccatcaaagg
    13141 tgttagagaa gacgtcactc aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta
    13201 tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga cctgctacag taactgctgg
    13261 cgatattatc gttgatagtg atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt
    13321 ctctgaagga gctacgttcc atgctcgctt aacagtgaaa cctggacgtg gttatgttca
    13381 agcagatgaa aataaaaaag aagatatgcc aattggcgtt cttccagtcg attcaatcta
    13441 cacacctgtt cgtcgtgtga actatcaagt tgagaacaca cgtgttggac accgtgaaga
    13501 ttttgataaa ttgacgatgg agatttggac agatggttca attgaaccgt tggaagcaat
    13561 gagtttgtct gcgaaaatca tgactgaaca tttggatatt tttgtgaact taactgatga
    13621 agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga
    13681 aatgacgatc gaagagttag acttgtctgt tcgctcttac aattgtttga aacgtgcagg
    13741 aatcaatact gtacaagaac ttacaaataa atctgagcct gaaatgatca aagtccgtaa
    13801 cttaggacgt aaatcattag aagaagttaa agcaaaattg cacgacttag gtttaggttt
    13861 acgtaaagaa gattaaaacc ttttacgaag gagggaaacc aacgtgagtt atcgtaaact
    13921 aggacgcaca tctagccaac gtaaagcgat gttgcgtgat ttaacaactg atttattaat
    13981 caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa
    14041 aatgatcact ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg cggctttcgt
    14101 acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt
    14161 acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg
    14221 cattttgaag acagaaccaa gacgcggtga tggtgcacca atggttgtct tagaacttgt
    14281 ctaaaatcac actaataaca tcactttata gatgattctt ttagagtgtt atgatgttgg
    14341 aagatatttt tccgagtcta gctcagcgct gctcctcaag cgaatatcgc ttggggagta
    14401 agcactcatc ataaagtgtt tttttatacc ttcaattacc tgtcatgaca gatatgtttg
    14461 aaggcataaa aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt
    14521 gttagtagga actttaggat gaagaaggag taatcatgga gccaattatt gaattagaaa
    14581 agataaatta taaataccag ccagatgatc ttcgccccgc attgaaagat gtctccttta
    14641 caattgataa aggtgagtgg atcgctatta ttggacacaa tggttcagga aaatcgacac
    14701 ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc
    14761 aaatattgga tgaagaaaat atttggacga tccgacagat ggttggaatg atttttcaaa
    14821 atccagacaa tcagtttgtt ggttcgacag tagaagatga cgttgctttt ggtcttgaaa
    14881 atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta
    14941 gaatggctga gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg
    15001 ttgccattgc cggcgtggtt gccttacgac ctgatatcat tattttagat gaagcaacca
    15061 gtatgctaga tccagaaggt cgtgaagaag tgatctctac gatcaagaag atcaaagaag
    15121 aaagtcaatt gaccgttata tctatcactc atgatattga tgaagcagct aatgccaatc
    15181 ggatactagt gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt
    15241 ctgctggtcc tgaactgatc gacttaggtc tagatctacc atttcctgaa aaactgaaga
    15301 gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg
    15361 tggattggtt atggacatcc gttttgaaca agtagacttc acttatcagc cgaatacacc
    15421 atttgaacaa agagccttat ttgacatcaa tatgacgatc aaagaaaaca gttatactgc
    15481 attagtaggg cataccggaa gcgggaaatc aaccttactt cagcatttga atgcgcttgt
    15541 taagccaaca agcggtacag tccatattgg agaacgagat attcagccgg atacggataa
    15601 taagaattta aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca
    15661 gctattcgaa gaaacggtag caaaagacat tgctttcggt cctaaaaact ttggtgtcag
    15721 cgaagaagaa gcactagtcc tagcaaaaga aacattagaa caagttgggc tggatgaaag
    15781 ctatttggaa cgttcgccat ttgaactttc aggagggcaa atgcgtagag tagcaatcgc
    15841 tggtgtgctt gccatgaggc cggaagtact tgtattggat gaaccaacgg caggacttga
    15901 tccccagggc agaaaagaaa tgatggagat gttttggcgt cttcataagg aacagcagat
    15961 cactattgta ttagttacgc atttaatgga tgatgtagcc aattttgcag attatgtcta
    16021 tgtgttggaa aagggacgaa tcgttaatag cggggagccg caagaggtct ttcaaaacat
    16081 tgaatggcta aaagaaaaac aactaggcgt acctactgcc accgagtttg cagaagaatt
    16141 aatggctaaa gggatgaact ttgctacctt gcctttgaca gctgaagaat tagcagacgc
    16201 tatcgtccat tatgctggag gaaatgccca tgatgaataa attgatattt ggtcgttata
    16261 tgcctggaga ttccttgatc catcgattgg atccccgagc taaattatta gccagttttt
    16321 attttatcgg aatcattttt ttagcaaata attgggaaac gtaccttttg ttggcatttt
    16381 tcacattact ttctgtcttc ttgtcaaaag tagatttgaa attttttatc cgaggcattc
    16441 gcccattgat ctggctaatc ctcttcactg tagctttgca ggtcttgttt acaagtggcg
    16501 gagaaactta ttggagttgg ggtgttttcc atatcactga atttggtgtg atgaacggcc
    16561 tctttatttt ctgtcgattc gtgttgatca tttttatgtc gactctactc acgcttacta
    16621 caccgccttt ggaattatcg gatgcgattg agtacttgct tcgtccattg aaagccgtac
    16681 ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat
    16741 tgatggacga aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg
    16801 aaggaaacct agttcagaaa atgaaagcag tcattccttt gctgattcct ttatttgtca
    16861 gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc tacaaaggtg
    16921 gggaaggaag aacgaaatat cgtgtattgc attggcataa tagagacacg atggttatca
    16981 ttgcttttgt attattaact gtgctactga tctttttaag aggataaacg ttcagtgggc
    17041 cgctatctat tcttttcttg ctgatctgtt tgcaagaaag gatgcaagca gtgtatgaca
    17101 agaaatatta gggtctgtgg cagactaagg tcacaggcct ctttctttta tctgaaaaga
    17161 aagaacgaaa aaattttata gaaaaatcat tattccagat aaccttttag gaacaaagga
    17221 gagaaaaaca tggtacgtta caaagcaatc attgcgtacg acggaacaaa ttttaatgga
    17281 ttccaaaaac agccaaatgg gcgtactgtt caagaagaag tagaaaaaac attacaaaaa
    17341 atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa ggacagatgc cggagtacat
    17401 gcgatcgggc aagtaatcca ttttgattat ccagaggagc gtccgttaga acgaatgcgt
    17461 ttcgcgctag atacccagtc accagaggat attgctgtaa gacaagtaga aattgtttca
    17521 gaagacttcc acgcaagata tttggtcaaa gaaaagacgt atcaatttcg agtggatata
    17581 gggaaaccac gcagtccttt tcgacgccat tatgcaagtt attttcctta cccacttgat
    17641 ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga ctcacgattt cacctctttt
    17701 tgtgcatcag gaagctcgat agaggataaa atacgtacga tctacgaggc taagatggag
    17761 gtaaatgaaa caaaagacga attgttgttt acatttaggg gaaatggctt cttgtataag
    17821 atgatacgga tcttagtagg aacgcttttg aaaattggca atggccgatt gcctgaagat
    17881 agtatcccag atattattgc gaaaaaagat cgtaatgcag caggacctac agcacaccct
    17941 gaaggacttt atttgtatga agtgaaatat gaataagagg ttatgataga agtggtcagc
    18001 ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa aattttgcaa aatttcggct
    18061 tattttcgaa gaagctactt ctggaacacc gtttaagaaa gaggtcgtga gcctgtcatt
    18121 taactctgag tcacaaagag caatttcaaa aaacagcttc tcatctttga tgaatatcgt
    18181 gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt tttctgtaac
    18241 caaaaaacga tggaacgttg ttttgattgc ccttggtgcg gacagtctga acaggttcca
    18301 tcgtttcttt attttctacg tcttcttctg tgtgtgtttc gtggttgttc ttctattgct
    18361 ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt accagacata aagaataaac
    18421 aatgcaatac ataggacaaa aaggattgca cctatcgtaa atccttcagg caagtaactg
    18481 aaagtgacag tatgtttacc tgctggaact tttatcatca agaatgcgtc tttgaaattg
    18541 ctgaccttga ctggtttccc atcaatttta gctttccatc ctttgtcata aggaatagtt
    18601 gtcaccaaca tggaatcttc agcggtattt acagtcccag aagcttttct gccagtggct
    18661 tccatttcga cacctttttc tttgatctga ttcattgcgg cttgataggc tacaacatct
    18721 agaccaacga ctttaggctc catgaaactt atttctttcg tcccataaaa acttgcagta
    18781 aatgtcactg ttgtctcttg aggataatag ccaagatcat agtattgacc agaaatgttg
    18841 atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg cactagaact ttctaattgc
    18901 gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat ttgcaggcac atggacttcc
    18961 caagtgagct cttttggctg gttatctgct gtttctgagt aagtgacgcc agtcgttgtc
    19021 tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg tatagaatgt aaaatactgt
    19081 tcatttagac cagacaatgt attgatcaag tttgtctgac tggtcaaatt atctgtcact
    19141 ggctgctcaa catcattaac ggacaacggt gcagtaaatg ctagtggcaa tgcattgaca
    19201 ttctgataca gtgtatagtc gccgctttga ccagcttggc ggaagccata tttactgaac
    19261 gtgctttcgt ttttagaaat gttatatttg attcctgtaa acccatccat cagcaaagta
    19321 ttattgttat aacggatatt caaatttgtc cctcttgaac ggaaacctaa tttatccagg
    19381 taagaggaag aattacgatt tcttatagaa gaaaacaaac tgataccact gtaaccatag
    19441 ttgatcgaat cgtttgcaga tactggatcc agattttcca agcggtaaaa cgagtcggaa
    19501 tctttttttg tttcatttac aagagctttc aagtcaggat aaggatcagt atataaactg
    19561 cgggaagcat aattccaatc atcaagaatg ccatggatca tactgttcgt attgaccagt
    19621 gcttcagcag acatgatcaa taataacaat atactcagat gattgaatga aagtttttta
    19681 tgctgataaa atcccacagc aaaagcataa atccctaaga aaaccactgt caatacaaag
    19741 gaagtcatcg tcacataggt gtagcttcct tcaggtttga tgccccatgc caccgcaaag
    19801 agagcagcta cgaccaaaat gattcctaac aacagcccac catcgtcttg ttctatcttt
    19861 tcccatcctt ttcctgcaag caggagcact aagaaagaga acagataggc ataacggaat
    19921 aaaaacatat tcggtgcatg cataccatgc cagaacaaat tcaatggaac aagataaaaa
    19981 cttgcgataa gtatggcgaa aagactgccg tacaaaaatt tatttttcaa cggtactttc
    20041 cgactggcaa aataaaacag acagaggact aatggtagca gacctgcgta aacgaacgga
    20101 atcgatccgt attttgtcgt gtcataaaca ccaatcatat ttttcatgat tagatccaga
    20161 taagaagtcg cttctgtttt caacgtagtg atctgactca gtgcctcgcc atttgaccga
    20221 agatccagca ctgctggcaa tacaatgatc attgaagcgc cgcctgccag taaagaagtg
    20281 ataccataag gaacaatcgt ttttttatat tctgaccagt ttgtcaatag acgggcgata
    20341 aaatataaga aagaaaatac gccaatcata aaacccatat aaaaactgga taaaaacaag
    20401 agtaaataac tgataaataa aagtttcggc cgtttaagat ccataacacg atgaatcccc
    20461 aaaatcacta atggaagata aacaaatgca tccagccaca tgatgatctc cgaatgtgca
    20521 gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa catgccccca gcgaggaatc
    20581 ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac agccgacctt aagcaaagtc
    20641 agaacataca gtgcatctgg catcatctgg tttggaaaaa agaacaccag tggcgtgaag
    20701 atcccgccta gatagtaaga aattaaagat aaataattca atcctagaga agcattccat
    20761 gtataaaaaa tactttgctt tccaagaaga acatttcgga agctggcatg aaaattagaa
    20821 aactgtgaaa aggcatcact tgccaaaata cttctgctgc ttccaggata gattccgata
    20881 cttagataaa caagtgctaa aatcaaaaac ggaatcagga aactagctgc tgcgtagcgt
    20941 ccgttttctc taaagaatga ctttaaattt tttttcatat ttattttcca c
//
LOCUS       DACDCV010000044        20556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDCV010000044 DACDCV010000000
VERSION     DACDCV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.044"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IV041_001580"
     CDS             297..1406
                     /locus_tag="IV041_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8382362.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IV041_001581"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IV041_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IV041_001582"
     CDS             2401..3225
                     /locus_tag="IV041_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382363.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IV041_001583"
     CDS             3257..4567
                     /locus_tag="IV041_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8382364.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IV041_001584"
     CDS             4715..5974
                     /locus_tag="IV041_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8382365.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IV041_001585"
     CDS             5984..6856
                     /locus_tag="IV041_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382366.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IV041_001586"
     CDS             6868..7698
                     /locus_tag="IV041_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382367.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IV041_001587"
     CDS             7738..9921
                     /locus_tag="IV041_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8382368.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IV041_001588"
     CDS             9982..11202
                     /locus_tag="IV041_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8382369.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IV041_001589"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IV041_001589"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8382370.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="IV041_001590"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="IV041_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="IV041_001591"
     CDS             14164..14373
                     /locus_tag="IV041_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8382371.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="IV041_001592"
     CDS             14366..14515
                     /locus_tag="IV041_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8382372.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="IV041_001593"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="IV041_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IV041_001594"
     CDS             complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IV041_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8382373.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17037..17270
                     /locus_tag="IV041_001595"
     CDS             17037..17270
                     /locus_tag="IV041_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8382374.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17267..17674
                     /locus_tag="IV041_001596"
     CDS             17267..17674
                     /locus_tag="IV041_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8382375.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18811..19329)
                     /locus_tag="IV041_001597"
     CDS             complement(18811..19329)
                     /locus_tag="IV041_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8382376.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            20447..>20556
                     /locus_tag="IV041_001598"
     CDS             20447..>20556
                     /locus_tag="IV041_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8382377.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         6979 a         3170 c         3825 g         6582 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
    16621 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
    16681 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
    16741 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
    16801 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
    16861 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
    16921 ttaattatat agaaagaaca atctttctat agagttagac atgagaggga ctatgcttga
    16981 tttaaaatca acaagcgtat atactgtata tacaaagaaa aaggagggag aagactatgg
    17041 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
    17101 agtttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
    17161 ttcgagcggc aattacaaaa aaacaagcct tatctgatga aattgattta gcaatatctc
    17221 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
    17281 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
    17341 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
    17401 ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta tcctacttat
    17461 acaattgata aagaaacatc cttttcacaa tgcaaataaa cgtactgctt tcttagctac
    17521 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
    17581 tttagcggta tatattgcta cgtatgataa ggatttcgat caactaaaat atgaagtgat
    17641 caacactata aagacacata cgcagcccat ttaaggagtt actgtatgtg tctttatagg
    17701 gttattagct gctatagcga aataattgaa gtaaattaca aatagctata attcaaatcc
    17761 aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
    17821 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
    17881 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
    17941 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
    18001 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
    18061 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt
    18121 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
    18181 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
    18241 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
    18301 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
    18361 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
    18421 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
    18481 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
    18541 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
    18601 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
    18661 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
    18721 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
    18781 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
    18841 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
    18901 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
    18961 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
    19021 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
    19081 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
    19141 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
    19201 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
    19261 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
    19321 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
    19381 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
    19441 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
    19501 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
    19561 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
    19621 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
    19681 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
    19741 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
    19801 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
    19861 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
    19921 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
    19981 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
    20041 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
    20101 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
    20161 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
    20221 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
    20281 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
    20341 atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac tagacagaaa
    20401 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
    20461 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tagctggaaa
    20521 gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DACDCV010000045        20428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDCV010000045 DACDCV010000000
VERSION     DACDCV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.045"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IV041_001599"
     CDS             24..1103
                     /locus_tag="IV041_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8382378.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1300..2001)
                     /locus_tag="IV041_001600"
                     /pseudo
     CDS             complement(1300..2001)
                     /locus_tag="IV041_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2015..2939)
                     /locus_tag="IV041_001601"
                     /pseudo
     CDS             complement(2015..2939)
                     /locus_tag="IV041_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3056..4135
                     /locus_tag="IV041_001602"
     CDS             3056..4135
                     /locus_tag="IV041_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8382379.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4167..4760
                     /locus_tag="IV041_001603"
     CDS             4167..4760
                     /locus_tag="IV041_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382380.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4870..5097)
                     /locus_tag="IV041_001604"
     CDS             complement(4870..5097)
                     /locus_tag="IV041_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8382381.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5200..7788)
                     /locus_tag="IV041_001605"
     CDS             complement(5200..7788)
                     /locus_tag="IV041_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8382382.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7808..8467)
                     /locus_tag="IV041_001606"
     CDS             complement(7808..8467)
                     /locus_tag="IV041_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8382383.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8516..9019)
                     /gene="trmL"
                     /locus_tag="IV041_001607"
     CDS             complement(8516..9019)
                     /gene="trmL"
                     /locus_tag="IV041_001607"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8382384.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9240..10106)
                     /locus_tag="IV041_001608"
     CDS             complement(9240..10106)
                     /locus_tag="IV041_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8382385.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10215..10856
                     /locus_tag="IV041_001609"
     CDS             10215..10856
                     /locus_tag="IV041_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8382386.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10918..12279)
                     /gene="mgtE"
                     /locus_tag="IV041_001610"
     CDS             complement(10918..12279)
                     /gene="mgtE"
                     /locus_tag="IV041_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8382387.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12297..13196)
                     /locus_tag="IV041_001611"
     CDS             complement(12297..13196)
                     /locus_tag="IV041_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8382388.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13198..13995)
                     /locus_tag="IV041_001612"
     CDS             complement(13198..13995)
                     /locus_tag="IV041_001612"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8382389.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13973..14677)
                     /locus_tag="IV041_001613"
     CDS             complement(13973..14677)
                     /locus_tag="IV041_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8382390.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14808..15383
                     /locus_tag="IV041_001614"
     CDS             14808..15383
                     /locus_tag="IV041_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8382391.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15501..16178
                     /locus_tag="IV041_001615"
     CDS             15501..16178
                     /locus_tag="IV041_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8382392.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16257..18065)
                     /gene="pepF"
                     /locus_tag="IV041_001616"
     CDS             complement(16257..18065)
                     /gene="pepF"
                     /locus_tag="IV041_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8382393.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18241..19239)
                     /locus_tag="IV041_001617"
     CDS             complement(18241..19239)
                     /locus_tag="IV041_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8382394.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19465..20118)
                     /locus_tag="IV041_001618"
     CDS             complement(19465..20118)
                     /locus_tag="IV041_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8382395.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5913 a         4443 c         3640 g         6432 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgta gtggctaact taaacttgaa atttacggac gtaaagcttg ctgccaaatt
     1321 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
     1381 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
     1441 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
     1501 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
     1561 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
     1621 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
     1681 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
     1741 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
     1801 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
     1861 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
     1921 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
     1981 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
     2041 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
     2101 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
     2161 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
     2221 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
     2281 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
     2341 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
     2401 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
     2461 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
     2521 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
     2581 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
     2641 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
     2701 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
     2761 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
     2821 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
     2881 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
     2941 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
     3001 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
     3061 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
     3121 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
     3181 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
     3241 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
     3301 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
     3361 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
     3421 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
     3481 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
     3541 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
     3601 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
     3661 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
     3721 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
     3781 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
     3841 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
     3901 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
     3961 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
     4021 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
     4081 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
     4141 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
     4201 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
     4261 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
     4321 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
     4381 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
     4441 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
     4501 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
     4561 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
     4621 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
     4681 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
     4741 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
     4801 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
     4861 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
     4921 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
     4981 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
     5041 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
     5101 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
     5161 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
     5221 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
     5281 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
     5341 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
     5401 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
     5461 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
     5521 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
     5581 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
     5641 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
     5701 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
     5761 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
     5821 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
     5881 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
     5941 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
     6001 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
     6061 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
     6121 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
     6181 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
     6241 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
     6301 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
     6361 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
     6421 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
     6481 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
     6541 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
     6601 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
     6661 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
     6721 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
     6781 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
     6841 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
     6901 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
     6961 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
     7021 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
     7081 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
     7141 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
     7201 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
     7261 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
     7321 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
     7381 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
     7441 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
     7501 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
     7561 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
     7621 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
     7681 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
     7741 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
     7801 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
     7861 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
     7921 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
     7981 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
     8041 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
     8101 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
     8161 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
     8221 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
     8281 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
     8341 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
     8401 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
     8461 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
     8521 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
     8581 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
     8641 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
     8701 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
     8761 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
     8821 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
     8881 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
     8941 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
     9001 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
     9061 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
     9121 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
     9181 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
     9241 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
     9301 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
     9361 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
     9421 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
     9481 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
     9541 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
     9601 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
     9661 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
     9721 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
     9781 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
     9841 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
     9901 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
     9961 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    10021 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    10081 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    10141 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    10201 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    10261 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    10321 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    10381 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    10441 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    10501 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    10561 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    10621 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    10681 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    10741 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    10801 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    10861 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    10921 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    10981 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    11041 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    11101 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    11161 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    11221 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    11281 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    11341 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ccaaaaacaa
    11401 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    11461 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    11521 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    11581 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    11641 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    11701 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    11761 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    11821 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    11881 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    11941 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    12001 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    12061 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    12121 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    12181 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    12241 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    12301 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    12361 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    12421 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    12481 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    12541 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    12601 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    12661 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    12721 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    12781 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    12841 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    12901 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    12961 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    13021 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    13081 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    13141 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    13201 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    13261 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    13321 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    13381 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    13441 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    13501 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    13561 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    13621 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    13681 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    13741 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    13801 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    13861 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    13921 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    13981 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    14041 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    14101 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    14161 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    14221 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    14281 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    14341 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    14401 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    14461 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    14521 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    14581 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    14641 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    14701 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    14761 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    14821 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    14881 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    14941 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    15001 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    15061 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    15121 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    15181 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    15241 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    15301 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    15361 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    15421 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    15481 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    15541 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    15601 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    15661 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    15721 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    15781 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    15841 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    15901 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    15961 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    16021 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    16081 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    16141 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    16201 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    16261 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    16321 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    16381 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    16441 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    16501 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    16561 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    16621 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    16681 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    16741 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    16801 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    16861 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    16921 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    16981 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    17041 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    17101 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    17161 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    17221 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    17281 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    17341 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    17401 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    17461 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    17521 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    17581 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    17641 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    17701 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    17761 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    17821 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    17881 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    17941 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    18001 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    18061 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    18121 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    18181 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    18241 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    18301 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    18361 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    18421 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    18481 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    18541 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    18601 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    18661 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    18721 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    18781 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    18841 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    18901 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    18961 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    19021 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    19081 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    19141 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    19201 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    19261 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    19321 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    19381 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    19441 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    19501 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    19561 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    19621 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    19681 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    19741 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    19801 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    19861 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    19921 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    19981 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    20041 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    20101 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    20161 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    20221 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    20281 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    20341 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    20401 tggaaaaact atctttgtaa tagtcttt
//
LOCUS       DACDCV010000046        20410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDCV010000046 DACDCV010000000
VERSION     DACDCV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.046"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..542
                     /locus_tag="IV041_001619"
     CDS             105..542
                     /locus_tag="IV041_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382396.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            637..1521
                     /locus_tag="IV041_001620"
     CDS             637..1521
                     /locus_tag="IV041_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8382397.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1745..4912
                     /locus_tag="IV041_001621"
     CDS             1745..4912
                     /locus_tag="IV041_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382398.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4932..5477
                     /locus_tag="IV041_001622"
     CDS             4932..5477
                     /locus_tag="IV041_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8382399.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5505..5960
                     /locus_tag="IV041_001623"
     CDS             5505..5960
                     /locus_tag="IV041_001623"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8382400.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5957..6715
                     /locus_tag="IV041_001624"
     CDS             5957..6715
                     /locus_tag="IV041_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8382401.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6801..7541)
                     /locus_tag="IV041_001625"
     CDS             complement(6801..7541)
                     /locus_tag="IV041_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382402.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7602..8855
                     /locus_tag="IV041_001626"
     CDS             7602..8855
                     /locus_tag="IV041_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8382403.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9177..9404
                     /locus_tag="IV041_001627"
     CDS             9177..9404
                     /locus_tag="IV041_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8382404.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9527..9853
                     /locus_tag="IV041_001628"
     CDS             9527..9853
                     /locus_tag="IV041_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8382405.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9921..10634
                     /locus_tag="IV041_001629"
     CDS             9921..10634
                     /locus_tag="IV041_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8382406.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10944..11795
                     /locus_tag="IV041_001630"
     CDS             10944..11795
                     /locus_tag="IV041_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8382407.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11916..13781
                     /locus_tag="IV041_001631"
     CDS             11916..13781
                     /locus_tag="IV041_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8382408.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13829..15238
                     /locus_tag="IV041_001632"
     CDS             13829..15238
                     /locus_tag="IV041_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8382409.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15346..16047
                     /locus_tag="IV041_001633"
     CDS             15346..16047
                     /locus_tag="IV041_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8382410.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16206..16685
                     /locus_tag="IV041_001634"
                     /pseudo
     CDS             16206..16685
                     /locus_tag="IV041_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17533..18108
                     /locus_tag="IV041_001635"
     CDS             17533..18108
                     /locus_tag="IV041_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8382411.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18108..18548
                     /locus_tag="IV041_001636"
     CDS             18108..18548
                     /locus_tag="IV041_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8382412.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18564..19265
                     /locus_tag="IV041_001637"
     CDS             18564..19265
                     /locus_tag="IV041_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8382413.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19753..20178)
                     /locus_tag="IV041_001638"
     CDS             complement(19753..20178)
                     /locus_tag="IV041_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382414.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6830 a         3397 c         4315 g         5868 t
ORIGIN      
        1 attttaaaaa ttttttttac ttagaatcca gcgaccctag atatttttct gtataatctt
       61 aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag tggttttgca
      121 aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc acaaataaaa
      181 aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt tccgttttgg
      241 tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc cgtgacatat
      301 taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc gctataaggt
      361 ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg tgtttttatc
      421 tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct tttatttcct
      481 atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt gtcaaaaaat
      541 aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt atgctatcgt
      601 tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt attagtggta
      661 aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat agcaaaattg
      721 gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga tgcaaaaaac
      781 tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg cggagatgga
      841 acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa tttcggattt
      901 ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct agaacctgag
      961 gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg aaaaataaat
     1021 gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat caatgatgta
     1081 gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg aatcaaacag
     1141 ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa ggaagaaatt
     1201 gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga aacattactt
     1261 ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga ttcttctttg
     1321 atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc tacgaaaaat
     1381 ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga agaattgacg
     1441 acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt attgccctca
     1501 catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg gagaacaccg
     1561 cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt tgtacaaaaa
     1621 agaaatttac acctaataga aaaatttttc cataaaaata catattttaa atatgaaagt
     1681 acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga gaggatggtg
     1741 gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct tccctctctt
     1801 gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc aatcagagaa
     1861 tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa ctacagaatc
     1921 tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg agacctcaga
     1981 agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg tttcagcgga
     2041 tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag acgaatcagt
     2101 aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga tcaaacgaga
     2161 tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata tttatatcaa
     2221 tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac atccaggtgg
     2281 attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg atttttgggg
     2341 aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt tgtatgatgg
     2401 gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg gtgtttccgg
     2461 tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta aagcctattt
     2521 ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac cgtatctagg
     2581 gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa cagcgccagg
     2641 aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag atggcgttag
     2701 cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag tcagtggttc
     2761 gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca atcgcggaat
     2821 catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga aaatcaccat
     2881 caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa tccatgtatc
     2941 aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg ggaacaatgg
     3001 gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat cagttatcga
     3061 catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta cgcaaaagct
     3121 gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca aatcttacgt
     3181 gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg gttttctaga
     3241 aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg atatacgcgc
     3301 agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta cggcaggtga
     3361 aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca tcatcagtgg
     3421 ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa acaaacggat
     3481 tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag attcttcttt
     3541 aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat tcgataactt
     3601 tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg ggaccgactt
     3661 aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc agacaggtgc
     3721 cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga ttgaatcctt
     3781 aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc ccgaaggtgt
     3841 cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac gtattactta
     3901 cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac aaacagatga
     3961 aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct tgttcgaagt
     4021 tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg ttgctgctaa
     4081 aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga cagtcgatgt
     4141 gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt ctcgcacgat
     4201 cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag ctgttttgtc
     4261 agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa atcaactttt
     4321 tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta ctgctaaggg
     4381 tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa gtgatacgcc
     4441 agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag tagaaggtgg
     4501 tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa ctggtttgac
     4561 acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg atcagcgaga
     4621 aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa atgatgcagg
     4681 attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat tagtgaatga
     4741 agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag attattcaga
     4801 atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc agatagctgg
     4861 taattactca gcagagataa catggacatt agcagatgcg ccgacagaat aaagatcaag
     4921 gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc tctttactta
     4981 tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc gaattcaatt
     5041 ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca tatataaatg
     5101 taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac cgaacagata
     5161 aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac ggtgtgatcg
     5221 aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg aaaaacttag
     5281 caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag acactttcac
     5341 tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg tttcaagaaa
     5401 aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt tctcaagtga
     5461 aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa ttaaaaaaag
     5521 cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa aatccaaatg
     5581 cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa acaaaagagc
     5641 cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat tttgatttac
     5701 ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt gtgaagggtg
     5761 cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat gatgcagcgg
     5821 accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt gactggcgat
     5881 ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt attttaaaag
     5941 aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag ctggaagtct
     6001 attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta cagaaggaac
     6061 gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag ttcctcctgt
     6121 tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg gaacatccgg
     6181 attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt taggtacagc
     6241 aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac aaacacaagt
     6301 acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga aattatcagt
     6361 caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg gttcaaaggt
     6421 gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa tgccatctac
     6481 cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag tagatctagt
     6541 aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag agaatgtgga
     6601 acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg tagacaacta
     6661 cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag cgtaaaatca
     6721 tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat ctgctatgct
     6781 tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg gaaacgttcc
     6841 tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa aaattctaac
     6901 acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta agattttaaa
     6961 agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga agtattcact
     7021 aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt gatactttca
     7081 atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc aataaagatt
     7141 ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac ttgacgaatt
     7201 tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt ccgattgatg
     7261 atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg tgcatttttt
     7321 ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc gatatcgata
     7381 aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat ttgagagata
     7441 gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat gatccattcg
     7501 ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct cctattaaaa
     7561 aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca acagtgacac
     7621 tttatttgac ctttactttg attttagatt taggtatcta tttattcttt tcgaaacaac
     7681 tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt ttacttccta
     7741 tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt ttttatttta
     7801 ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct atcactggac
     7861 tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat cttggaacag
     7921 caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa agtatagaca
     7981 aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat tttggaggta
     8041 tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt cacttccgct
     8101 catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt tctttgtttt
     8161 tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa tcgcttcaac
     8221 tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat gattataaaa
     8281 gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg ttggaatttt
     8341 tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata caacagctaa
     8401 caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa aaagcagaat
     8461 cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc ccgattgatc
     8521 tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat catcgtgtag
     8581 ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca cgttttagta
     8641 tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa agaggaaaaa
     8701 cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg gatgtttacg
     8761 aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt gtagaaatgt
     8821 atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga taagtttgag
     8881 gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac agaagcgtga
     8941 tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag ttgacacttc
     9001 cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa gaagtgaaaa
     9061 gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa agggaagagc
     9121 aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca aaaaaaatgc
     9181 tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa gcctataacc
     9241 aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta agagagattc
     9301 tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc tcaagggaag
     9361 aagacacgct actgcataca catggtggct acctaaatta ttgattttct atgacattta
     9421 ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt atgtaaaaaa
     9481 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
     9541 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
     9601 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
     9661 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
     9721 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
     9781 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
     9841 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
     9901 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
     9961 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
    10021 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
    10081 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
    10141 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
    10201 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
    10261 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
    10321 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
    10381 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
    10441 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
    10501 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
    10561 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
    10621 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
    10681 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
    10741 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
    10801 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
    10861 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
    10921 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
    10981 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
    11041 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt ttaagagatt
    11101 cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat
    11161 gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata
    11221 tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc
    11281 aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag
    11341 cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag
    11401 ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga
    11461 cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca
    11521 tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg
    11581 cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag
    11641 ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac
    11701 aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa
    11761 gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa
    11821 acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt
    11881 tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca gttggtcgtc
    11941 gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc
    12001 ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta aaagaagacc
    12061 cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt ggcagtaatg
    12121 tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg gaggatggaa
    12181 aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt atatttacac
    12241 cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca ttggcaacgg
    12301 tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct gcagcagatg
    12361 gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag ttcaaagcca
    12421 accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc acagcagttg
    12481 ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca ccatctggtt
    12541 atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa ttagagccat
    12601 tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt gtcgttttag
    12661 tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt gccggtaatg
    12721 ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca ggtctaatca
    12781 tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc gtaccgatca
    12841 tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt cttccagcca
    12901 tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat acgaaacttc
    12961 gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa ccaactgtgt
    13021 atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct ggaggtattg
    13081 gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg gtcagcttat
    13141 tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg acagtagcag
    13201 taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt ttaggatttg
    13261 acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga aaaccgatta
    13321 cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca ttgagtgaag
    13381 taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt gatccagaag
    13441 taggagaact agttgcacct gcagacggag agatcacgac tatttttcca actggacatg
    13501 ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg atggatacag
    13561 tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc gtcaaagcag
    13621 gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat agcgtaatca
    13681 ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta gaccaaaaag
    13741 agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat caggatgcaa
    13801 tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc aattagaagg
    13861 ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg gaggtaagca
    13921 gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag aaaaatatat
    13981 ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt
    14041 tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa tttttccaaa
    14101 tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc tgattgatga
    14161 gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa tgcctcttca
    14221 tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct atgaacgatt
    14281 tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga ctttcaatga
    14341 aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc
    14401 aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc
    14461 cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga ttatctatgc
    14521 aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga tccaaaacca
    14581 agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg catacacagc
    14641 gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt
    14701 ccagttgcta aaagaatatc cagtggacta catcggattt agttattata tgtctacggc
    14761 aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa
    14821 aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag ggctgagaat
    14881 cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg aaaacggtct
    14941 tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc ggatcgatta
    15001 tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag atttgatggg
    15061 ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg
    15121 ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga atcgatcaaa
    15181 gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa acttgtaatt
    15241 aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct ttttttattt
    15301 tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa aaggacacgt
    15361 gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg aaaatatcca
    15421 gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc
    15481 actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa gaaaagtggt
    15541 cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc ggcaagcttt
    15601 acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga tttgcaattg
    15661 gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt
    15721 aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta tcttccacaa
    15781 gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg tttgctgacg
    15841 aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt tttaacagaa
    15901 gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat acggaaatcg
    15961 tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac ggagctgcat
    16021 ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc
    16081 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct
    16141 tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa gggggtgtgg
    16201 cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct tcgatggtaa
    16261 ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa aatatactca
    16321 atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt cagatcacag
    16381 agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca acgggagtcg
    16441 gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt attccggcat
    16501 cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt gcattgttag
    16561 ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta gatccgaatg
    16621 cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat ttgacaatta
    16681 gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct tgagacagga
    16741 atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt ttaggagcga
    16801 tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac
    16861 ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt
    16921 tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga aattattctt
    16981 catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca ggcaggcgca
    17041 acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga tatttttaca
    17101 aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa caaaaatgat
    17161 caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat ttatcagtga
    17221 aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta tttgttgttt
    17281 aaaataatac acgttattgg catataggac gaaatcccag tatcttctat aagtaagtca
    17341 tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg gctgaaaaat
    17401 gatttttttc taaatactat atatagtggc gtctatccta tataccacta aaaaactaca
    17461 aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt ttgagttagg
    17521 gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa taaagaaagt
    17581 tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat cgaactggtt
    17641 tacggtggtt catactccgg gattatggga gtgattagta aaacagtact ggaaaataac
    17701 ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc tggaaaagat
    17761 gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt atttgaaaag
    17821 ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt ctcacaactt
    17881 ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt gaatattggc
    17941 ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga ttttatggat
    18001 aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt caatgaatta
    18061 cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg agtcaaatat
    18121 atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt atcgaaaaag
    18181 cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac caagaaagca
    18241 attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat atggaagaac
    18301 tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca atcgatccag
    18361 gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc attgtacaag
    18421 aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat ttaaaatcag
    18481 accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac gtaggcgagt
    18541 acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg attggtgcag
    18601 gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca ttttatgaaa
    18661 atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag tacgcttttt
    18721 tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct ttagtgaacg
    18781 atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat ctaaacgctg
    18841 atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta gatacgatgc
    18901 taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc catatcaaag
    18961 tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat gaacagctag
    19021 aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat aatcaatggt
    19081 atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc gattttgtag
    19141 aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta gctgaattgg
    19201 aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt caagaattag
    19261 gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt agaataagcc
    19321 tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct ttactctttg
    19381 atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc aaaggaagaa
    19441 gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt tgtttatctt
    19501 cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa tgaatctttg
    19561 actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa ttctgtatgg
    19621 atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca tttcgtctcc
    19681 tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca aaagaagatc
    19741 caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac ggtaattacc
    19801 gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa taatatattc
    19861 gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag atgattcaga
    19921 agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat ctgtaaatgt
    19981 caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata cactgttggt
    20041 ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg aggattggtc
    20101 agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca aaataaatag
    20161 tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat tataccattg
    20221 gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac aaatagagta
    20281 aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa gcgttttaaa
    20341 aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc aaatacttgt
    20401 ttaagtataa
//
LOCUS       DACDCV010000047        19996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDCV010000047 DACDCV010000000
VERSION     DACDCV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.047"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IV041_001639"
     CDS             complement(<1..626)
                     /locus_tag="IV041_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8382415.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IV041_001640"
     CDS             899..3370
                     /locus_tag="IV041_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8382416.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IV041_001641"
     CDS             complement(3473..5209)
                     /locus_tag="IV041_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8382417.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IV041_001642"
     CDS             complement(5206..5910)
                     /locus_tag="IV041_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382418.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6543
                     /locus_tag="IV041_001643"
                     /pseudo
     CDS             6041..6543
                     /locus_tag="IV041_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            6503..6841
                     /locus_tag="IV041_001644"
     CDS             6503..6841
                     /locus_tag="IV041_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8382419.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6862..8280
                     /gene="ffh"
                     /locus_tag="IV041_001645"
     CDS             6862..8280
                     /gene="ffh"
                     /locus_tag="IV041_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8382420.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8392..8970
                     /locus_tag="IV041_001646"
     CDS             8392..8970
                     /locus_tag="IV041_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8382421.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8974..9495
                     /locus_tag="IV041_001647"
     CDS             8974..9495
                     /locus_tag="IV041_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8382422.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9574..10128)
                     /locus_tag="IV041_001648"
     CDS             complement(9574..10128)
                     /locus_tag="IV041_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8382423.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10381..10656
                     /gene="rpsP"
                     /locus_tag="IV041_001649"
     CDS             10381..10656
                     /gene="rpsP"
                     /locus_tag="IV041_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8382424.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10668..10919
                     /locus_tag="IV041_001650"
     CDS             10668..10919
                     /locus_tag="IV041_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8382425.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11044..11835
                     /locus_tag="IV041_001651"
     CDS             11044..11835
                     /locus_tag="IV041_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382426.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12005..12526
                     /gene="rimM"
                     /locus_tag="IV041_001652"
     CDS             12005..12526
                     /gene="rimM"
                     /locus_tag="IV041_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8382427.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12516..13277
                     /gene="trmD"
                     /locus_tag="IV041_001653"
     CDS             12516..13277
                     /gene="trmD"
                     /locus_tag="IV041_001653"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8382428.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13413..13760
                     /gene="rplS"
                     /locus_tag="IV041_001654"
     CDS             13413..13760
                     /gene="rplS"
                     /locus_tag="IV041_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8382429.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13892..15028)
                     /locus_tag="IV041_001655"
     CDS             complement(13892..15028)
                     /locus_tag="IV041_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382430.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15144..16058)
                     /locus_tag="IV041_001656"
     CDS             complement(15144..16058)
                     /locus_tag="IV041_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8382431.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16149..16577)
                     /locus_tag="IV041_001657"
     CDS             complement(16149..16577)
                     /locus_tag="IV041_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8382432.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16582..16956)
                     /locus_tag="IV041_001658"
     CDS             complement(16582..16956)
                     /locus_tag="IV041_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382433.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17242..17427
                     /locus_tag="IV041_001659"
     CDS             17242..17427
                     /locus_tag="IV041_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382434.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17439..17687
                     /locus_tag="IV041_001660"
     CDS             17439..17687
                     /locus_tag="IV041_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382435.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17718..17897
                     /locus_tag="IV041_001661"
     CDS             17718..17897
                     /locus_tag="IV041_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382436.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17940..18410)
                     /locus_tag="IV041_001662"
     CDS             complement(17940..18410)
                     /locus_tag="IV041_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382437.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18497..19195
                     /locus_tag="IV041_001663"
     CDS             18497..19195
                     /locus_tag="IV041_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8382438.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19373..19714
                     /locus_tag="IV041_001664"
     CDS             19373..19714
                     /locus_tag="IV041_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382439.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19707..>19996
                     /locus_tag="IV041_001665"
     CDS             19707..>19996
                     /locus_tag="IV041_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8382440.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6560 a         3509 c         3933 g         5994 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaatggtgcc
     6421 ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg
     6481 ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt
     6541 tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc
     6601 agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata
     6661 tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt
     6721 ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa
     6781 gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata
     6841 aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg
     6901 atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg
     6961 gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact
     7021 aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa
     7081 caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag
     7141 ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt
     7201 aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg
     7261 ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt
     7321 caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc
     7381 cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca
     7441 ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg
     7501 caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta
     7561 gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg
     7621 gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc
     7681 atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc
     7741 cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac
     7801 gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac
     7861 tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg
     7921 atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt
     7981 gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg
     8041 ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg
     8101 aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg
     8161 gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc
     8221 aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa
     8281 gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag
     8341 caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt
     8401 ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct
     8461 gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg
     8521 agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat
     8581 atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga
     8641 attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa
     8701 attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc
     8761 gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa
     8821 ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct
     8881 tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag
     8941 gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct
     9001 ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact
     9061 ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg
     9121 ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag
     9181 aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta
     9241 aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg
     9301 gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg
     9361 tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca
     9421 tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat
     9481 tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt
     9541 acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc
     9601 tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt
     9661 ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac
     9721 attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc
     9781 tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct
     9841 tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt
     9901 aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg
     9961 agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa
    10021 tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta
    10081 agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct
    10141 ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact
    10201 tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa
    10261 tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag
    10321 ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata
    10381 atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc
    10441 gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac
    10501 aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta
    10561 tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg
    10621 caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa
    10681 gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag
    10741 acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg
    10801 gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg
    10861 ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa
    10921 taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga
    10981 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    11041 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    11101 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    11161 ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat
    11221 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    11281 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    11341 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    11401 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    11461 atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    11521 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    11581 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    11641 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    11701 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    11761 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    11821 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    11881 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    11941 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    12001 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    12061 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct
    12121 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    12181 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    12241 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    12301 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    12361 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    12421 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    12481 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    12541 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    12601 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    12661 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    12721 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    12781 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    12841 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    12901 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    12961 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    13021 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    13081 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    13141 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    13201 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    13261 cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag
    13321 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc
    13381 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    13441 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    13501 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    13561 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    13621 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    13681 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    13741 tcaaagaaat ccgtcgttaa tcatcgttat cagactgaga aagaaccctt ggtattcaag
    13801 ggttcttttt ttgttctctg tttttttata ttaacgattt ctatttggga catttttgat
    13861 taataaaaag gggcaaaaaa ggggcaacaa gttacagttt ttctatatct tctttcaatt
    13921 tttcttttac tccctttgtc acatgcaaat aaatcttttc agtgatatca ctattttcgt
    13981 gcccaacgcg atcctgaata gcatacagag gagttcctag ttccgctaat tttgaaatat
    14041 gggtatgtct aaaaatatgt gaactaagtt ttttatcaat cttcatgtca gctttatgat
    14101 ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt tcctttcgtt gtttgaaata
    14161 gaaattgacc attcggattt aattctagaa gctcattgta aatagctatc gctttttttg
    14221 gtaaatcaat ttctcgcatt ccagcagcag tttttgtaga atcagatttt ttcatatcag
    14281 ctattgaacg ttctctatac atcatcgtcc catttataac tactgatgca ttattattgg
    14341 taatgtgtac atcgtcttta cttaatgcga ttgcttctcc aggcctcatt ccagtcaaat
    14401 atagccactg gaaaaggaga gaatatcttt tattgtgtga agttgtgaaa tttactagtc
    14461 ttttatattc atcatcttct aaaaacttat ctttaatttt tatcgttttt gactctcgtt
    14521 tataatcaat aactacttcg ttgataggat ttttttctac atagcctttt ttcatagcgt
    14581 atgaaaaaag aagattcaat ttggatttaa ttacactgac gtacttattt gacaagtcat
    14641 ctttatatat catatcttca aaaatattat ttaaatctac tgtattaata ccagaaacga
    14701 tgtaagtctc aggtatcttt ttctttatag tgtttaagat attattcgta ggataatatg
    14761 tagactcctt aacttgtctt ttgtaaataa ctaaccattc ttcgacaagc tcatgaaatg
    14821 ttagatcagg cttctgaagt gttttttcgt ttaattttat atcaattttt ttatttaact
    14881 ctagcaatgc cactttctga gtttctcgag atttattttt ataagtaatg ctcacttttt
    14941 ttctttttct agtttttgga tcaatatatc gctcattata tttatatact ttttttccgt
    15001 ttttatcagt tttggtttca atccacattg ttttcatctc ctatttgttg ctataatagg
    15061 aatggataag taagccaatt atagcaggtt tatttttcat cacgtccaca aactttggcg
    15121 agggaggggg cgtgtttttt tgtttagtag ctacctttcc atgaagtatc tgcgaagaac
    15181 acttcaaatg ttgttccatc atttttcact tcaaaatatg ctgttccttg tgcggattta
    15241 cccggttgga tggtttccga aaagtaatct ctactagcta aatcagattt aatatctgat
    15301 gaatcataaa attctaaata acttgaactt gcatcaaaag gtgtagtccc tgtgttttct
    15361 acaagaaaat ctacctttgc gtaaaatagg ccttctggtt tatagaaatc atcaccgtaa
    15421 tcttttgata cagactggac agttacgtta atagagttac cattttcaga agtaaaagga
    15481 actgcttctc ctatactatg tgcaactgat gccgtctggg gttctgaaga cgaagttgta
    15541 gttgtagttg tagtagagct tgaatctttg gattttccat ctttatctaa gaaagttcgt
    15601 tgattataat atacaacctg ttcagttttt ggactaaccc aaattaacat tgtttcatca
    15661 tcagaatcag ttgaataagt atattggagc atctctaatt tttttatatc ttttgcacta
    15721 tttccatgtt caattaattc agagtgctct ttataataat ctccttgtgc atcctttgaa
    15781 atagatacaa ttaattcatc ccgtgtgata gcattgtcta attctttgta aacaaaatca
    15841 tttgtccatt tacgtaacgg tgaaccaagt gtctgataaa cttgagttaa atcatcacca
    15901 acttttattt tatgaacgtc tgatgaagtt agtttttttg tttcatattc agatgttgat
    15961 gaaacagtat tttctttagt gctagcagaa ttaggactag tggagcatcc gcttaatacc
    16021 aagcctaaaa aaagcaaccc aaaaacaatt tttttcattt ttaattcctc acttctatga
    16081 tatgattttt ttgtaggatc ttagaaataa ggtttttagt ccgtgttgca gcacggactt
    16141 tttttacatt atataagaaa gcgataagcg ctttctggaa gtccgtaaag attctttaat
    16201 tcctcaattc gtttaggata ttgatcattg tcttctttgt atagagaaac aatgagatta
    16261 gcagcaaagc aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacatag
    16321 taactggata agccacgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat
    16381 ctttcctcag agtctctcag ttcatcattt aagaagatta ttgcacgacc tctaatttct
    16441 tgaaactgtc ctttgggatt ctcgataaaa ggaacgtatt gaattttaat gcccatcttt
    16501 tcacaaatat aaaaaggatt agcggactgg tatttccgct tcaactcctc gactaaatta
    16561 atcgtatcca tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat
    16621 atcccagaag gttccaataa ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt
    16681 ttctccgcca taagacatat tgacatttga atctagtaat ttatcaagtt caactacttc
    16741 atcttttgtt gcccagtcag gaacttggtt tctaccgagt aaataatcag tagttacatt
    16801 aaaataatct gctacaagtt gtagtctttt agtactagga gttctatttt tccattgata
    16861 aatcgtgttt ttaggtatat ttaattcttc ttccaattga gttatactta tgcctctttt
    16921 atgagctaat tcctttattc tatctagtaa attcattttc gcacctcaca agctacgaaa
    16981 acactaataa aaaaattagt taaaggtgtt gacaactaat aaaagtgtta gtatactgtt
    17041 ttcgtaagct aaaatattag ctaaaaagac ttgataacta ataaacactt cacggtcggc
    17101 aaactctgaa atgtaaatta ctaggcattt ctgtgtctta tttagctatg tctatatact
    17161 aataaaaata ttagttattg tcaacgaata ttagctaatt ttttagctta cagattattt
    17221 ttttagaaag gagctatttt tatgtctgag aatttagact taaaaattcg agcggagatg
    17281 agaaaaagaa gaatgacttt caaagaacta gctgcgctta tcggtatttc aggagcttat
    17341 ttatcagata ttctaaacgg caatcgtgat ggaaagaaag cacaacagca tatcgaaaca
    17401 gtgaaaaaaa tattggacat ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag
    17461 attttgtaaa aaaatacaat tattctccat ctacttatca acgtcgaatg tcggaactaa
    17521 aaaatacagc aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca
    17581 atacagaatt atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa
    17641 aggtaacacc taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt
    17701 ttgagaggtg aaggttaatg ggtaaattca acagagcatt ggtattcagc gcaccgctaa
    17761 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
    17821 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
    17881 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
    17941 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
    18001 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
    18061 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
    18121 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
    18181 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
    18241 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
    18301 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
    18361 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
    18421 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
    18481 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
    18541 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
    18601 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
    18661 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
    18721 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
    18781 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
    18841 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
    18901 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
    18961 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
    19021 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
    19081 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
    19141 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
    19201 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
    19261 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
    19321 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
    19381 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
    19441 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
    19501 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
    19561 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
    19621 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
    19681 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
    19741 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
    19801 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
    19861 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
    19921 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
    19981 gacacacgga aaccga
//
LOCUS       DACDCV010000048        19587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDCV010000048 DACDCV010000000
VERSION     DACDCV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.048"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IV041_001666"
     CDS             <1..152
                     /locus_tag="IV041_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382441.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IV041_001667"
     CDS             complement(230..763)
                     /locus_tag="IV041_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8382442.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV041_001668"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV041_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8382443.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV041_001669"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV041_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8382444.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IV041_001670"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IV041_001670"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8382445.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IV041_001671"
     CDS             complement(4488..5066)
                     /locus_tag="IV041_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8382446.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IV041_001672"
     CDS             5388..5858
                     /locus_tag="IV041_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8382447.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IV041_001673"
     CDS             complement(5966..7192)
                     /locus_tag="IV041_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8382448.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IV041_001674"
     CDS             complement(7946..8146)
                     /locus_tag="IV041_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8382449.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IV041_001675"
     CDS             complement(8280..8888)
                     /locus_tag="IV041_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8382450.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IV041_001676"
     CDS             complement(8973..9443)
                     /locus_tag="IV041_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8382451.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IV041_001677"
     CDS             complement(9491..10405)
                     /locus_tag="IV041_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8382452.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IV041_001678"
     CDS             10551..11354
                     /locus_tag="IV041_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8382453.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IV041_001679"
     CDS             11532..12479
                     /locus_tag="IV041_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8382454.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IV041_001680"
     CDS             complement(12547..12840)
                     /locus_tag="IV041_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8382455.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IV041_001681"
     CDS             complement(12868..13533)
                     /locus_tag="IV041_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8382456.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IV041_001682"
     CDS             complement(13673..14305)
                     /locus_tag="IV041_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382457.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IV041_001683"
     CDS             complement(14312..15379)
                     /locus_tag="IV041_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8382458.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IV041_001684"
     CDS             complement(15376..16107)
                     /locus_tag="IV041_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8382459.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IV041_001685"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IV041_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8382460.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IV041_001686"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IV041_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8382461.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IV041_001687"
     CDS             18270..19439
                     /locus_tag="IV041_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8382462.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         5911 a         3937 c         3137 g         6602 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggt
//
LOCUS       DACDCV010000049        19471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDCV010000049 DACDCV010000000
VERSION     DACDCV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.049"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(19..993)
                     /locus_tag="IV041_001688"
     CDS             complement(19..993)
                     /locus_tag="IV041_001688"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8382463.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1073..1948)
                     /locus_tag="IV041_001689"
     CDS             complement(1073..1948)
                     /locus_tag="IV041_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8382464.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2078..3985
                     /locus_tag="IV041_001690"
     CDS             2078..3985
                     /locus_tag="IV041_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8382465.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4526..7804)
                     /locus_tag="IV041_001691"
     CDS             complement(4526..7804)
                     /locus_tag="IV041_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8382466.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8075..8620)
                     /locus_tag="IV041_001692"
     CDS             complement(8075..8620)
                     /locus_tag="IV041_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8382467.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8632..9324)
                     /locus_tag="IV041_001693"
     CDS             complement(8632..9324)
                     /locus_tag="IV041_001693"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8382468.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9343..9627)
                     /locus_tag="IV041_001694"
     CDS             complement(9343..9627)
                     /locus_tag="IV041_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382469.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9640..10203)
                     /locus_tag="IV041_001695"
     CDS             complement(9640..10203)
                     /locus_tag="IV041_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8382470.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10431..11093
                     /locus_tag="IV041_001696"
     CDS             10431..11093
                     /locus_tag="IV041_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8382471.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11217..12413
                     /locus_tag="IV041_001697"
     CDS             11217..12413
                     /locus_tag="IV041_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8382472.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12514..13890)
                     /locus_tag="IV041_001698"
     CDS             complement(12514..13890)
                     /locus_tag="IV041_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8382473.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13927..14793)
                     /locus_tag="IV041_001699"
                     /pseudo
     CDS             complement(13927..14793)
                     /locus_tag="IV041_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14882..15907)
                     /locus_tag="IV041_001700"
     CDS             complement(14882..15907)
                     /locus_tag="IV041_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8382474.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15925..16599)
                     /locus_tag="IV041_001701"
     CDS             complement(15925..16599)
                     /locus_tag="IV041_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382475.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16783..17676)
                     /locus_tag="IV041_001702"
     CDS             complement(16783..17676)
                     /locus_tag="IV041_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8382476.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17750..19447)
                     /locus_tag="IV041_001703"
     CDS             complement(17750..19447)
                     /locus_tag="IV041_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8382477.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3444 g         6154 t
ORIGIN      
        1 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
       61 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
      121 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
      181 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
      241 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
      301 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
      361 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
      421 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
      481 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
      541 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
      601 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
      661 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
      721 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
      781 gtcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
      841 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
      901 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
      961 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
     1021 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
     1081 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
     1141 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
     1201 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
     1261 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
     1321 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
     1381 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
     1441 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
     1501 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
     1561 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
     1621 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
     1681 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
     1741 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
     1801 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
     1861 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
     1921 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
     1981 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
     2041 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
     2101 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
     2161 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
     2221 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
     2281 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
     2341 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
     2401 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
     2461 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
     2521 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
     2581 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
     2641 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
     2701 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
     2761 actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata aagtgatcgt
     2821 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
     2881 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
     2941 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
     3001 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
     3061 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
     3121 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
     3181 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
     3241 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
     3301 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
     3361 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
     3421 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
     3481 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
     3541 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
     3601 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
     3661 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
     3721 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
     3781 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
     3841 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
     3901 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
     3961 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
     4021 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
     4081 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
     4141 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
     4201 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
     4261 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
     4321 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
     4381 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
     4441 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
     4501 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
     4561 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
     4621 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
     4681 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
     4741 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
     4801 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
     4861 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
     4921 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
     4981 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
     5041 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
     5101 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
     5161 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
     5221 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
     5281 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
     5341 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
     5401 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
     5461 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
     5521 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
     5581 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
     5641 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
     5701 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
     5761 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
     5821 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
     5881 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
     5941 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
     6001 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
     6061 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
     6121 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
     6181 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
     6241 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
     6301 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
     6361 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
     6421 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
     6481 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
     6541 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
     6601 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
     6661 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
     6721 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
     6781 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
     6841 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
     6901 gatcgtaata atatgactct aaaccagtat tcgttttttt catctctacg tccagtttag
     6961 taatcgagtt cgtggccgga aacaaattcc catgaccgtt tccgaatata ttataagaac
     7021 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
     7081 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
     7141 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
     7201 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
     7261 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
     7321 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
     7381 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
     7441 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
     7501 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
     7561 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
     7621 tatcttcact agttcttggt acttcctcca ttgttgacat cccttcaatg ttcacgtgtg
     7681 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
     7741 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttta
     7801 acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt acctaattta
     7861 tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa gaaaaaatat
     7921 aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa
     7981 taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt
     8041 tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg
     8101 actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggcatctt
     8161 tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga
     8221 tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt
     8281 ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct
     8341 cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc
     8401 ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat
     8461 gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag
     8521 tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa
     8581 caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag
     8641 atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc
     8701 gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa
     8761 tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa
     8821 tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc
     8881 acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat
     8941 ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg
     9001 tttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat
     9061 gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt
     9121 cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc
     9181 ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc
     9241 gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat
     9301 tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc
     9361 taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt
     9421 ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt
     9481 gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc
     9541 ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct
     9601 tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag
     9661 ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc
     9721 ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc
     9781 taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc
     9841 aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt
     9901 ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat
     9961 ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag
    10021 aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccaccaggat gaaataccag
    10081 ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat
    10141 ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag
    10201 catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc
    10261 tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa
    10321 tccttcttta gtccgatact ctttctagta gtccgtatgc taaaataagt atgtaatcga
    10381 ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac
    10441 gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt
    10501 tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg
    10561 gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa
    10621 ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga
    10681 atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta
    10741 aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg
    10801 tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc
    10861 tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga
    10921 cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac
    10981 aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg
    11041 cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt
    11101 gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct
    11161 aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg
    11221 atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat
    11281 tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg
    11341 atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc
    11401 aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca
    11461 gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg
    11521 tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc
    11581 aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg
    11641 ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa
    11701 ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga
    11761 tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg
    11821 ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa
    11881 gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc
    11941 tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc
    12001 aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag
    12061 catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc
    12121 ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat
    12181 tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac
    12241 gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc
    12301 aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc
    12361 ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct
    12421 ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt
    12481 ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc
    12541 atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca
    12601 tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aagatggaga cgattggcaa
    12661 tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa
    12721 cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc
    12781 cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata
    12841 agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa
    12901 gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac
    12961 cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac
    13021 atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc
    13081 tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat
    13141 tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc
    13201 aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa
    13261 aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa
    13321 cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc
    13381 ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta
    13441 tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact
    13501 tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa
    13561 aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata
    13621 cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag
    13681 tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc
    13741 attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat
    13801 tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag
    13861 attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa
    13921 cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca
    13981 accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc
    14041 acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag
    14101 tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat
    14161 gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa
    14221 gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac
    14281 gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc
    14341 aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa
    14401 atacgcaccg cttacaagaa gagctaaagc aataataccc tatacataat ctttcgtttg
    14461 tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt
    14521 agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg
    14581 tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac
    14641 aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag
    14701 tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc
    14761 caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat
    14821 aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt
    14881 tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt
    14941 ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta
    15001 aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc
    15061 ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg
    15121 aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg
    15181 tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg
    15241 tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat
    15301 ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt
    15361 agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga
    15421 tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg
    15481 aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa
    15541 tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac
    15601 ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca
    15661 aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct
    15721 tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat
    15781 aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca
    15841 atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa
    15901 aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc
    15961 gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt
    16021 caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg
    16081 acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct
    16141 caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc
    16201 attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc
    16261 attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc
    16321 catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg
    16381 tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt
    16441 aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa
    16501 atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg
    16561 aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct
    16621 tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt
    16681 cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc
    16741 aagcagctta aatttattat gttattctta ataattggtc tattatctgt tatttttctt
    16801 tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag
    16861 caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat
    16921 ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt
    16981 gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc
    17041 taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat
    17101 ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa
    17161 gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc
    17221 agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt
    17281 ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg
    17341 ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc
    17401 tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc
    17461 atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata
    17521 attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt
    17581 aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat
    17641 ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa
    17701 aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag
    17761 acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt attccgatta
    17821 gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg
    17881 gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc
    17941 cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg
    18001 gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc
    18061 catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg
    18121 ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac
    18181 ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat
    18241 atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat
    18301 aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg
    18361 cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat
    18421 ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca
    18481 cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct
    18541 cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg
    18601 tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag
    18661 gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa
    18721 tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta
    18781 cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat
    18841 tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca
    18901 ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt
    18961 ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag
    19021 agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt
    19081 ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa
    19141 gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg
    19201 ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa
    19261 ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa
    19321 tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga
    19381 aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt
    19441 tattcatttt tttctccccg ttcttttttt a
//
LOCUS       DACDCV010000050        19168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDCV010000050 DACDCV010000000
VERSION     DACDCV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.050"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(183..1751)
                     /locus_tag="IV041_001704"
     CDS             complement(183..1751)
                     /locus_tag="IV041_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8382478.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1755..2036)
                     /locus_tag="IV041_001705"
     CDS             complement(1755..2036)
                     /locus_tag="IV041_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8382479.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(2033..2956)
                     /locus_tag="IV041_001706"
     CDS             complement(2033..2956)
                     /locus_tag="IV041_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8382480.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2953..3831)
                     /locus_tag="IV041_001707"
     CDS             complement(2953..3831)
                     /locus_tag="IV041_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8382481.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3818..5155)
                     /locus_tag="IV041_001708"
     CDS             complement(3818..5155)
                     /locus_tag="IV041_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8382482.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5158..6261)
                     /locus_tag="IV041_001709"
     CDS             complement(5158..6261)
                     /locus_tag="IV041_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8382483.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6335..7843)
                     /locus_tag="IV041_001710"
     CDS             complement(6335..7843)
                     /locus_tag="IV041_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8382484.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7809..8501)
                     /locus_tag="IV041_001711"
     CDS             complement(7809..8501)
                     /locus_tag="IV041_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8382485.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8523..9497)
                     /locus_tag="IV041_001712"
     CDS             complement(8523..9497)
                     /locus_tag="IV041_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382486.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9797..10669
                     /locus_tag="IV041_001713"
     CDS             9797..10669
                     /locus_tag="IV041_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382487.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11251..13449)
                     /locus_tag="IV041_001714"
     CDS             complement(11251..13449)
                     /locus_tag="IV041_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382488.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13681..14673
                     /locus_tag="IV041_001715"
     CDS             13681..14673
                     /locus_tag="IV041_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8382489.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14733..16154
                     /locus_tag="IV041_001716"
     CDS             14733..16154
                     /locus_tag="IV041_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8382490.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16147..17646
                     /locus_tag="IV041_001717"
     CDS             16147..17646
                     /locus_tag="IV041_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8382491.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17685..19079
                     /gene="uxaC"
                     /locus_tag="IV041_001718"
     CDS             17685..19079
                     /gene="uxaC"
                     /locus_tag="IV041_001718"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8382492.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5774 a         3895 c         3377 g         6122 t
ORIGIN      
        1 tgtttttata ttcacaatgt tggcttttta ttgcattatc ttgacagata aaaaacaaaa
       61 aataatctaa aaacattgat gcataagatt gataactttt tttacataac aatatataca
      121 aacaaaaaaa ttcactaact agtgaaaata agttagtgaa cctcatttat taaagaatta
      181 aattacagat gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt
      241 tttttattta gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt
      301 atacacatgt ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa
      361 tgaaactgtg ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt
      421 ggaatcgtcc aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat
      481 tttcccttgt cctcattcca agtaacataa caataaatat aattggattc atttaagtac
      541 agtaccagtc cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca
      601 tcaaaagtaa aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga
      661 gattcaccgg aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca
      721 tttggcaaaa tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata
      781 aactgatgat ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc
      841 ggagttgtct ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga
      901 atagcggttt ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc
      961 atgtaccatc ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta
     1021 gaagcaggtt tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca
     1081 gtaatattct ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc
     1141 gtcatcaaat aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat
     1201 tctgtacctg caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat
     1261 tcttgtaata cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca
     1321 ttgacaagcc atttttttcc cgtttcaaga tcatggaaca aagaaggatc aaagccgtta
     1381 ctatttaaat aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa
     1441 ttgtggacat ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac
     1501 aatccatcag cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc
     1561 aaagatactt gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac
     1621 tcatatactc ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct
     1681 ttcactctta atatattagg atctggattg aatcctctta aaataggatt tttcactttg
     1741 atagtctgca tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca
     1801 gctgctaaaa aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt
     1861 aataatccaa atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa
     1921 ctttttttaa actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa
     1981 atcaaagata gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct
     2041 gaaccccctg cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt
     2101 tattacgttc agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac
     2161 cagttgggtg aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac
     2221 gccacggtct tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt
     2281 caaaacgata ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat
     2341 ttttactggc taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata
     2401 tgaaatccac cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat
     2461 tggcagaagc cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg
     2521 tgcataaggt atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg
     2581 aattgaataa agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt
     2641 gaagattttc taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc
     2701 ctaactggct accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg
     2761 ttcctgctcc cacaagttta aaattggata atctgctttc tatatattca tcatatcttc
     2821 cttcttttac tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc
     2881 cttcttggat agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt
     2941 tgtctttcat cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg
     3001 tgtttccata tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga
     3061 ttgatacttt ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt
     3121 atcgtttttt ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa
     3181 cgttgtataa gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg
     3241 gcgatgtagc aaaagtgttt cgatgtccac ctgtgccgct agatcaatac aaactttgac
     3301 acgagaatcc aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt
     3361 tgcgagccgt tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac
     3421 atcataaata cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc
     3481 gctttcagaa atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata
     3541 tcccattcct gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat
     3601 caattcactt ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg
     3661 aaacggtcct tctttttctg gataagcata gagggctggt actgtttcca attcatttaa
     3721 gtacaattga tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc
     3781 actaggggta tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt
     3841 ttttacgaac aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac
     3901 tgatttcttt agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct
     3961 ggttaaagga aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta
     4021 ttcctgtcat catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat
     4081 tgtcaaatgt gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag
     4141 catggacatt ccttgccgtg atattcgaaa aatggatcct tctgaatgct gatgtccgtt
     4201 catctatcgg ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga
     4261 aataataaag attcagtata aatggacaca tcacattatc catgactata ttggatactc
     4321 gaatatcttc aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag
     4381 tttcttggaa gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc
     4441 ctaatacgac accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc
     4501 aagcgatcct ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca
     4561 cgtcaatatg gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg
     4621 tatttggtga atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa
     4681 gaatcgggtt gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc
     4741 gatgacagtt atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat
     4801 ttctaaaagt atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag
     4861 agatgttctc tacattttga gcatagatac aggaagcata aacttttcga tggactcctt
     4921 cccaacgtga atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc
     4981 ctgcgcttaa atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac
     5041 ctgctggaac aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta
     5101 ttgcttcagt attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta
     5161 taatgcctcc acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca
     5221 aataattggt tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa
     5281 ccctgctact tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa
     5341 ttcatcaagt aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag
     5401 cagtcccaaa cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc
     5461 cagataattt cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc
     5521 ttcttgatat ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca
     5581 atctgtttct tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat
     5641 cgatcggctc caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt
     5701 cttttcatcc catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact
     5761 gatttcaaac tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa
     5821 ttcagcataa aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat
     5881 ttctttatgc caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag
     5941 gagttctgct gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag
     6001 ctttcctgta tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc
     6061 atcttcttgc acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg
     6121 ggttcgttcc caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt
     6181 tcctttatac cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa
     6241 tttctttgac cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac
     6301 aaataccaag aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt
     6361 cctcgtactc ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt
     6421 tgaatccatc ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt
     6481 tttcaatttc tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca
     6541 ttacagcaaa ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg
     6601 tatctttgaa agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt
     6661 gttgccaaag atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg
     6721 ttccttcgat accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa
     6781 attgaagtaa tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca
     6841 agccgccaat accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga
     6901 tcttgtttac aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat
     6961 tcttgatatc caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct
     7021 gatcagtctt ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc
     7081 ttgaaaaatt caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat
     7141 tatcgtctac agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg
     7201 tataacgaat atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc
     7261 catcaggatc gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg
     7321 gaacatctag tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt
     7381 agtctttttg tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat
     7441 cttcagaaat ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata
     7501 cgcctgattt taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa
     7561 cgatatctcc tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat
     7621 caggaatcca gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg
     7681 tgtctgtagg cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat
     7741 cttctgtctc tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc
     7801 ctgcaaaact aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact
     7861 gttttaataa tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa
     7921 tggcaaaata tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata
     7981 aaggttaaaa cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat
     8041 atcaacacta atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg
     8101 aaaaaatatc ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc
     8161 acatatatga acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa
     8221 agatccacaa gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca
     8281 cgatagtttt ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag
     8341 cgcagccatt ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact
     8401 cccaaacctg cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata
     8461 cgatttagcc ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta
     8521 tcttagtaaa cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata
     8581 aacaatccta cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc
     8641 ttcaatccag ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa
     8701 ttcaataaca agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc
     8761 agcaaggtaa tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag
     8821 cgtcctgctc catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct
     8881 gaaaggtaga tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact
     8941 gtgcgtgtcc attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg
     9001 aattcattca atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt
     9061 acccaagaca tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg
     9121 ttcgttactt catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata
     9181 tttaatccga acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac
     9241 aaacgaatga aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga
     9301 taatcttgaa aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa
     9361 aggactcccg gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt
     9421 ttttttcgtc ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt
     9481 ttctttacac tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc
     9541 atgatagata actatctgaa ttacgtatat aatcattttt atcgtaagta aaaacgctta
     9601 catagcaatg atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta
     9661 cgcatagcaa accttctatt tttttctatc tgacacttag cataggaaat gattatatac
     9721 gtaatctttc accgatgata aattgaacat atagaaacag aaacatttaa tttattttga
     9781 ggaggagcct atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt
     9841 caacaatacg tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat
     9901 cgctacatct tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac
     9961 taccttttct ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact
    10021 tgctgtttcg attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact
    10081 gatggcctac ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt
    10141 tgtattttct atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct
    10201 agggctggtc aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat
    10261 gatcattatg agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat
    10321 ggatggttgc acagattttg gtgtattttt caaaattatg ctgccattag ctttaccgtc
    10381 tattgctact atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat
    10441 tttgtatatc aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat
    10501 tgtatcaagt gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac
    10561 agttaaaatg gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt
    10621 tcagaaatat tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga
    10681 acgcaaaatg gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga
    10741 aaattgcttc ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga
    10801 acaccgtgta tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc
    10861 acaaacattg cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc
    10921 ccatatttca caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc
    10981 gtaaaaaaga actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa
    11041 tttaactatc cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca
    11101 gtccacaaga aaaacagcct cttgattaaa cggcgtttat cttgttgatt ctgcggcaat
    11161 aaaatcagcc agcagcaaaa catgaagtgc tgttttttgc tgtcctcttt tattgcttga
    11221 atcagatcac aagaaaaaca gcctcttgat tcacaaatat ttttttcgat attctcccgg
    11281 cgtaatcgat tccttctttt taaagaaacg aataaaattt tgcggatttc gatattgcaa
    11341 tctttctgca atctctttta cggagaggtt agtctctttc aaccattttt ttgctacctc
    11401 taaacgatga ttttgaacaa aatcaccaaa tttttctcct gtttcctttt taaatacatt
    11461 acttaaatag ttagggttat aatgcaacct atcagcgatc acatctaaag acagatcacg
    11521 atcatattct gtgtagacga tatgcataat attctccgac aaatttttaa attcatgatc
    11581 agtgattgct tgagagcatt ctgtgattgg cagaactaat tgttctaaaa caagtttttc
    11641 tagttctttg ggatgataat tgttcagtgc ttttacataa atttgtttca tgttatcaaa
    11701 gttttttgaa tctactccca ataactgtcc taattgaacg agttcattga tcaagcggac
    11761 cgaaactact tcccgactca aaggattttt attttgtgca aacaatgcat caatcaattc
    11821 atgtactaaa ttcttcactt cctgatttcc agaacgaatg gcttcaaaca atcgattttg
    11881 ttcttctatc ggatatctaa taagtgtttt ttcatgagca gcaggaacga tttcctgata
    11941 aaaaataatt gaacttggac cagtatttac acgataatac agggcttctt ttgcccgatc
    12001 aaccgattgc ttgctttcat tcagcgtttc gaaccggtta ctgatgccta tactgatagt
    12061 taaattcaag tattctttta cacgtttttg aatgagtcgg caatactcca tgactttttt
    12121 gcttgcttct gcatcatctt tatccattcg ataaatagtg gcttgcattt cttcattcaa
    12181 aacgatcggc agcattcgat tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc
    12241 taaaagtagc aaatcacgct ccccaccgtg actatcttct aacagatcga tttgaaccaa
    12301 agctgtataa tagcagtcat tttgagaact ataaccaaat tgctgcaacc gctgatctag
    12361 ttctcgcctt tctactcgat ttctaaataa actaagtaca aatagcgttt ccagctgtgg
    12421 cttttgggta actaagtgtg cacttaacgc ttctttttct cctatgatcc gactgactga
    12481 ttgagcgacc aaatctagtt ctttcccact gaatcctttt gatttaagat tcagacgttc
    12541 ctgtatttga gaaatcggac gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat
    12601 gagtcctacc agtgttaaag acactgcaac tagcccgatt ctcagattgc ggatcattgc
    12661 gccaattgca ctagagtcaa cttctaatct gtaaatccaa ttattataat ctgatttcag
    12721 tactatatac gttttatctt tttctcttat cactttcagc tgattgtaat ctagatccac
    12781 gtccgttaat acagttggtt gctctttatt ttttactgtc tgagtagtaa aaaggttgcc
    12841 aacttcgttt tcaatagaaa gtatgtcttc tccactttca ccaacaactt gttctattgt
    12901 atgtttctct atcgtagcaa ttcccaatgc atatttttct ttttcaaaca ttggcaaaga
    12961 aatcaccatt tcgattcctt tgtttttctt ttgccaaaat aaattatttt tgttatttat
    13021 gtacttctgt tgatactttt caacttcctc ttcggtcagt tgactcaacg atccattgat
    13081 tactccccat ttaccagcca aactgactaa atcataatta ttatcatcca taacaatcgt
    13141 ttctataaaa tttagttctt tcctgatttc agaataagtg gagtaatctg tatatgtcag
    13201 cggatgcttg aatatgtccg agaaattttt ggtcgaacca tacgtagaaa acgaatattc
    13261 aatcgttcta attttccatt caacgttagt tttaacttgc ctgagcaaat tctttttgtc
    13321 tgttaaataa ctcgattcga tcgattcata ggtattctgg taaatgaata aactaaatcc
    13381 tatgacaagt acggctccta ataaaaacat cggtaaaaat attttataaa atactgtact
    13441 tttcttcatt ttcttcctct cctcctttta aaataaaagg ctttcatttt tctgtaaaat
    13501 tgatactcat ctaattaaat gagcattccc cgctttcaag aatagcataa ttaaaaataa
    13561 aaatccaagt aatttttaac gtgtgcaatc ttttcatatc aaagatctat tgacttatcg
    13621 tagttatcgt aatatgatga atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga
    13681 atggtaacga tcaaagatat cgctaaagca gctggcgttt ctcatacgac agtttctcgt
    13741 gctttgaata acagcagttt aataaaaaat gaaacaaaac aaaaaatacg acaactagct
    13801 aaggaaatga attattcgcc aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat
    13861 ctaatcggac tatttttctc aagtttgcat aaaggaactt catcaaattt cctagcagat
    13921 tccattgcag gcatccattc agtattagac acaaattaca gtttatctgt tgaaagtatc
    13981 gatgttgttg atttaacaga aatcaacctg caacgctacg atggactgat cgttatgagt
    14041 caaagtgatg atgatcagcc tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt
    14101 gttgtcaatc gtcacatcaa tgatccagcg gttatcaacg tcactgccga tgatgcggcc
    14161 ggtgttatgc aggcagtaga ttacggtatt ggatgcggac atacgaagat cgcttatatt
    14221 ggcggaaaga aaaattttcg ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta
    14281 aaaaaagcga gcatccctgt caatctagcc tattttttaa caggagatta cagcttagaa
    14341 agcggcctcc aaaacatgga atacctatta tcactttctg acctgcctac tctggttttt
    14401 tgcggcaacg acgacatagc gattggtgca ttgagagctg tacatttagc tggcctccgt
    14461 gttccccaga acatttctct gatcgggttc gatgattctc cagtcgtctc ctatctcaat
    14521 ccgccgctta ccaccgtacg taagcctttg aaagaaatgt gtcaagaagg aacaaagctt
    14581 ttactagatt tgatcgaagg caggacgatc gaacaatcta aaattgagct ctccaccttg
    14641 ttgatccttc gtgagtcgat caattacttg taaactacaa gtaattttat ataaaaatgt
    14701 taacgtgtgc ataacacaga aaggaagttt atatgaaaaa actgacaaaa gaattagtga
    14761 aaaaaaacga agcacctgta aaagttcttc agtttggtga aggcaatttt atgcgtggat
    14821 ttattgattg gcagattcag cagctgaata accaacaatt gtttaaaggt aatgttgcaa
    14881 tcattcaacc tttagaacaa ggtcttggaa atatgatgaa ccagcaagat aatttatata
    14941 cggtcatttt acaaggactc agcaatggcg aaatcgttga tacttccgag attatcaccg
    15001 tagtagaaca aatgatcaat ccttatgatg aatgggatgc ttatctatct ctagcagaaa
    15061 atgacgactt ggcctatatc gtctccaata caacagaggc aggaatccaa ttcgtggaga
    15121 ctgataattt aacagatact cccccacaga gcttcccggg caaactgaca gcctttttat
    15181 accgacgttt caaattaaac aaagctggct ttaccatcat tccttgcgaa ctgattgaca
    15241 gaaacggcga acgtctaaaa gaaatcattt tacgctacgc agaaaaatgg gatttagaac
    15301 ctgcttttgt tgagtggtta gaaaaagaaa atgttttctg ttgtagttta gttgatcgca
    15361 tcgtcccagg ttatcctcgc gaagatgcag gtgttttcaa tcaacggcat aactatgaag
    15421 atcaattgat ggtcaaagca gaaccattca tgctttgggt gatcgaaggg cctaaagaat
    15481 tggagaatca actaccattc aaacaggctg gcttgaatgt catcatcaca gatgatatga
    15541 ctccttatcg tgaacgtaag gtgcatttgc ttaatggacc gcatacagcg atggtccctc
    15601 ttgctttgtt ggcggaattg gaaactgtag aagaagtaat gaaagatcct ttattctccg
    15661 catatgttga tcagttattc aatctagaat tgattcctat gctttcttta cctaaagatg
    15721 aattagccat ttatgctgac caaatcaaag aacgtttctt gaatcctttt gcccatcaca
    15781 aattagaagc gatttcatta aatagtgttt caaagttcag cacccgtctg cttccagtgt
    15841 tcaagaagta tatcgaagaa caaaatcagg taccaccgtt aatcactgtt tcactagctg
    15901 cgctacttct tatgtatcga ggcgatcaag taaagccaca tgatgatgaa aagacgataa
    15961 gcgaatttac tgatgcttgg tctgataatg gaacagctat tcctcgatta ttgcaaaacg
    16021 ctgcgctttg gggagaagat ttgtctcaaa taccgaatgt gacagataca gttcaagaaa
    16081 tagctgcaaa gatcgatcaa aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga
    16141 tggtccatgc ttgaaaccct gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc
    16201 gtcgcattaa cccctattcc aaaacaaaca caactgacga ttgatcagca aacgatcacc
    16261 actcttgaag atatcccgca aggtcataag attgcattgg gtgatttgaa agctggagac
    16321 aacgtgatca aatatggcta tccaatcggt catgtgacga cagacgtaac tgccggtcaa
    16381 tggctgcaca ctcataatgt aaaaaccaat ctttctggtg aattggatta tcgatatgaa
    16441 aaagatgtcc atccaagtca ttaccttttt gagaaccgaa catttcaagg ttacttgcga
    16501 aaaaatggaa aagtagggat tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg
    16561 aatggcattg ctgaactgat cgtcaaacag ttcaaggaaa aacatccaga tctgggacag
    16621 tttgaccata ttacgatttt aaagcatcct tatggctgtt cgcagcttgg aaaagatcat
    16681 caaaatactc gagaaatttt agcggacgca gtaaatcatc ccaatgccgg aggagtttta
    16741 gtatttggat taggttgtga gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa
    16801 tatgatgaag aacgagtgaa atttctagtg gctcaagatg tttacgatga gatcgaacaa
    16861 ggtgtggctc tattagaaga gcttttaaca gcggcggaaa atgaccagcg catatcagtc
    16921 ccgctctcta agttgaatgt cggtttgaaa tgcggcggtt ctgatggtct atcgggaata
    16981 actgctaatc ctttacttgg tgcattttct gactacttga ttgctcaagg aggcagcacg
    17041 atcttaacag aagtgccaga aatgtttggc gcagaacaag tattgatggc acgagcggaa
    17101 aatgaagaag tttttgactc aatcgttgac ttgatcaatg acttcaaaca gtatttcttg
    17161 tcttacgggg aaccagttta cgaaaaccca tctcctggta ataaagcagg tgggatcact
    17221 acattagaag ataagtcttt aggctgcacg caaaaatcag gaagctctcc tgtcgttgat
    17281 gtgctacaat atggcgaaaa aatcagaaaa ccagggctta gtttattgca agctcccggg
    17341 aatgatcttg tagctgcctc tgctcttgct tcatccgatt gccagttggt cttgtttacc
    17401 acaggaagag ggactccgtt tggctcatat gtcccaacgg tcaaggtatc tacgaataca
    17461 actctttttg aacgcaaagg tcattggatg gattttaatg caggcgtctt attagaacaa
    17521 ccaatggaag taatcttaga atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa
    17581 gaaacgaaaa atgaacaaaa tgacgttcga gaaattgcca tttttaaaaa cggagtaaca
    17641 ctttgatttt cataaaacag tataaaaaga aagaaggaag atttatgttt ttatctgatg
    17701 attttttatt aaaagacgat tgggcgaaaa agctttacca ttcctacgca aaaaacatgc
    17761 ccattattga ttatcactgt cacttagatc caaaagaaat ttacgaaaac aacaatttca
    17821 gcaatctgac agaagcttgg ttaggaggcg atcattacaa atggcgcttg atgcgagctt
    17881 gcggcgtgcc tgaagaaaaa atcacaggaa atgcttctga ttttgaaaaa tttttagctt
    17941 ggtgtcaaac cgtaccaaaa attatcggaa atccgcttta cagttggtca catcttgaat
    18001 tgaagcgttt cttcggtatt gacttgttga taaatgaaga aaatgcagag aaaatttgga
    18061 cacaagcaaa tgaaattttg gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat
    18121 cgaatgtaga agtgatttgt acaacagatg atcccgcaga cgacttgcac tatcatcagt
    18181 tgcttgctca tgaagaacca gatttgaagg ttctaccttc attccgtcca gataaagcac
    18241 tcaatattga aaaagaagga tttaatgcat gggtcacact actggaagaa gcagctgacc
    18301 aaacgatcac cacctatcaa gaactgatag aagtcttagg gcagcggata gaatacttcc
    18361 atcaacatgg atgtcgtgtt tccgaccacg ctttagacat cttacgctat aaagaagcgg
    18421 atgaaacagt attagaagag atttttcaaa aagcacgagc taacaaacta ttatcttcag
    18481 atgaaatcga tgcttatctt acagaaacac tgatacattt gacccacttc tatcattctc
    18541 ataattggac gatgcagctg cacatccatg cttatcgtaa ctgtaataca caaatgttgg
    18601 aaaaattagg gcccgatacc ggctatgatg gaatgaatga tctttctttg actattcctc
    18661 tgcaaaaatt attgaaccgt gcagaagaaa cggatcagtt gccaaagacg atcctttatt
    18721 cattgaatcc gaatgactat cctgcgattt tagctttgat gggctgtttc caaaaagaac
    18781 agaatggaaa actgcagtta ggttctggat ggtggtacaa cgatactcga gctggtatgc
    18841 gcgatcaatt gacacaattt gctgatggta gtgctttggg gaattttgtc ggcatgctga
    18901 cagattcaag aagtttcctg tcctacacta gacatgaata tttccgtcgt gttctttgtg
    18961 aatttttagg cgaaatggtc atgcgaggag aagcacctga agatgaacaa cttttaggaa
    19021 gtctagttca agcgattagt tatacaaatg ctaaaaatta ctttggcttt tttgcctaat
    19081 tgattaatag taacagtaac gaatcaaaaa caggatagtg aaataacgga atccgtttat
    19141 ccacgtttta gagaagaaga cggaccaa
//
LOCUS       DACDCV010000051        19144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDCV010000051 DACDCV010000000
VERSION     DACDCV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.051"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(361..864)
                     /locus_tag="IV041_001719"
     CDS             complement(361..864)
                     /locus_tag="IV041_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8382493.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1223..2248
                     /locus_tag="IV041_001720"
     CDS             1223..2248
                     /locus_tag="IV041_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8382494.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2571..3449
                     /locus_tag="IV041_001721"
     CDS             2571..3449
                     /locus_tag="IV041_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382495.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3925..4041
                     /locus_tag="IV041_001722"
     CDS             3925..4041
                     /locus_tag="IV041_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8382496.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4969..5307
                     /locus_tag="IV041_001723"
     CDS             4969..5307
                     /locus_tag="IV041_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382497.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5401..5631
                     /locus_tag="IV041_001724"
     CDS             5401..5631
                     /locus_tag="IV041_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382498.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6031..6402
                     /locus_tag="IV041_001725"
     CDS             6031..6402
                     /locus_tag="IV041_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8382499.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6676..7368)
                     /locus_tag="IV041_001726"
     CDS             complement(6676..7368)
                     /locus_tag="IV041_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8382500.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7371..8156)
                     /locus_tag="IV041_001727"
     CDS             complement(7371..8156)
                     /locus_tag="IV041_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8382501.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8393..9793
                     /gene="lacG"
                     /locus_tag="IV041_001728"
     CDS             8393..9793
                     /gene="lacG"
                     /locus_tag="IV041_001728"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8382502.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9818..10135
                     /locus_tag="IV041_001729"
     CDS             9818..10135
                     /locus_tag="IV041_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8382503.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10148..11821
                     /locus_tag="IV041_001730"
     CDS             10148..11821
                     /locus_tag="IV041_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8382504.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11835..13466
                     /locus_tag="IV041_001731"
     CDS             11835..13466
                     /locus_tag="IV041_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8382505.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14338..15528
                     /locus_tag="IV041_001732"
     CDS             14338..15528
                     /locus_tag="IV041_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP8382506.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15662..17851
                     /locus_tag="IV041_001733"
     CDS             15662..17851
                     /locus_tag="IV041_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8382507.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            17848..19101
                     /locus_tag="IV041_001734"
     CDS             17848..19101
                     /locus_tag="IV041_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8382508.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILIVKLS"
BASE COUNT         6297 a         3057 c         3788 g         6002 t
ORIGIN      
        1 gagaagggag cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa
       61 caagcttttt atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt
      121 tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa
      181 aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg
      241 ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa
      301 aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt
      361 ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa
      421 aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag
      481 aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat
      541 gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa
      601 aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc
      661 aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa
      721 agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg
      781 aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa
      841 taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa
      901 accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa
      961 attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg
     1021 cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct
     1081 aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt
     1141 tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca
     1201 attactagga aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga
     1261 taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta
     1321 cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta
     1381 ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact
     1441 gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc
     1501 cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc
     1561 aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc
     1621 tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc
     1681 agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa
     1741 agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt
     1801 atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt
     1861 gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca
     1921 atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt
     1981 tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt
     2041 tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac
     2101 acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat
     2161 aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt
     2221 aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg
     2281 acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc tcagcattaa
     2341 gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat ttattctatc
     2401 aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca atgctttcct
     2461 tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt tagtgtacaa
     2521 taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca atgaggttca
     2581 aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa attctggcag
     2641 aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca aaaaacatcc
     2701 ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa gcaaaggcga
     2761 ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta gctatttatc
     2821 tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt ccaagaacta
     2881 tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc caagtaacag
     2941 gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc ttgatctttc
     3001 ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg gatctaagat
     3061 tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta tctcagaata
     3121 agataagaga tagttacgaa agtgccattc gtttttttga actttatttt cctgattacc
     3181 attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact aaatggttga
     3241 aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca gttgatgaac
     3301 aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata catgattatc
     3361 cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc gagcacattg
     3421 gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt taaaagagcg
     3481 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
     3541 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
     3601 aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tagtgttcta
     3661 tcgaacacac agtacgttca ataggtaaac tagctataac aatagatatt aaagttaacg
     3721 gacaagaggg ccttttttag gtagctaaaa cgtaataatg aaaatttatt tccagagata
     3781 ggagtcaatc agctagtctg atcgactgtt ttaacaagtt gactttcatt ttaacttttg
     3841 ataccattta aaacacaaat cgtaattgtt tcgatttgtg ttttttagca tgactacaat
     3901 caaagaagaa agtgggtcgt ccatatggaa aaaacagata tctcaagtgc ataccgccgc
     3961 ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa
     4021 cggaatagtg ggaaaaggta agaagaagga aacttaatta aaaaaatagc cgaaaaacgt
     4081 ttttcggcta ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat
     4141 tttccatttt taacatatga taaattggta gaatctttct tttcaaattg ataccattcc
     4201 ctaattccag ttatatagtt aactacagaa ccacaaagca cgtacgttta taaaagtatt
     4261 atctgtagtt atttttcaat tccctttcaa cctcttattt tgaccagtag tattgatcac
     4321 tttacctttt aacacctttg ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa
     4381 ctacataatc aaaggttttg cgaaatattt catgaaatat ttcatgtaga agcgagcaat
     4441 acggttgcaa ataaccatta tcgtttaata agcagttacg aaagctccca atatctctaa
     4501 cgttcagctc actccatttc ttttcttcaa aaaaatcagt gattcgtatc tctttctatt
     4561 tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc
     4621 agttactatt gctagaataa catcaaggaa taataagttt tggttttcag ataatagtcg
     4681 ataagttgtg tattggaatt ttagatacag taataaagta tttttcatga aattttgtga
     4741 gaatcactat tacaagtatc tttaataaaa ttaatggtta aggaggaact taaagtagaa
     4801 tgtaaatgaa tagttgtagt cagtcgattg tgatactaga ctcaaagttg caggaataag
     4861 tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa gggctttttt ttatactttg
     4921 ttgattgtag tgtttatttt ccctctgttt agccaagtaa cattttctat gactcaacca
     4981 gtttcaagtg aggaacatga taaccttttt cgtgctacag aagagtttaa gaaatatcag
     5041 gaaaatattg ttccggagac tccattagct agtgagccaa ttactgatat tgatggaaac
     5101 caaataggat ggtatataca gtacgaagtt atggtggtaa ataatgactc aatagtaaaa
     5161 aatacagtag gcttagagct aacaccgatt gaaaaggtga gttcattgtt aacgtatatt
     5221 tatactgatg aaagtgacga gactgagatc attttaacgg attactcaaa aattgctact
     5281 gataaaaagt atatattgtg gatgtagaca ctagtgaaat actttatgat atagaaattg
     5341 atgaaaattt actagatgta gcacaagaaa tcgatcatca aaagcaggaa gctattcgat
     5401 atgctggtag aaaagggaaa agaaaatatt tattacgtga atgtagcaaa agttagagaa
     5461 gatgaaaatg agtggaaaga attcaagagt cgctactcaa ttaactcgac tccgacattt
     5521 acagtttaca gagaaggatc gatagaaaaa actgtttttt ggactaaaga aagtggaatg
     5581 tctttagctg aagtagaaga gtttttggat tatgttagta tgcaacagta agggagttct
     5641 tattatgaaa aattttgaca catattatgt tattgttttt tcgatatctt tgctgttaat
     5701 attactaact ttaatgcagt ataaagagta tccagagtat tttattttaa cttttgtata
     5761 tttagtaagt agctacacac ttctatataa attcagaagt tcttctagag tttctaaagt
     5821 ttgtttctta attagcttat tgattctata atatttttga tttatatagg aaattaaaaa
     5881 tattatagaa tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat
     5941 aatgaaattt ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa
     6001 gattactttg cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa
     6061 gaagtcatca atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca
     6121 gttgccatta tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat
     6181 ggcgataaag acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag
     6241 gcagatctta agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca
     6301 gagaaagata cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt
     6361 tcaactcagc ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta
     6421 ataaaagtat atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt
     6481 gaaaacacta tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga
     6541 atgaaaaagt caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat
     6601 gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt
     6661 agttagttga atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc
     6721 agtaaaatct ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt
     6781 tttaccgaat ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa
     6841 tcctgagtaa gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc
     6901 cgctttaaga aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat
     6961 cttgtcccag tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc
     7021 tttgacagta gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt
     7081 cgcttcaagc ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata
     7141 atagctttct ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat
     7201 gaaatgtttc aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc
     7261 tgataaacca tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg
     7321 agagtccaaa aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct
     7381 atagtggtat tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct
     7441 tgaacctccc gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa
     7501 aacacataaa aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg
     7561 gaattattta gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct
     7621 tcgttgtgaa cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg
     7681 tcgttggtga tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt
     7741 agaatttctg gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg
     7801 atccgaattt tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct
     7861 aagaaaatcg tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt
     7921 tcttgaatct gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa
     7981 agactttgag cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa
     8041 tctctgcgaa cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg
     8101 attccttttt tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc
     8161 ttcacctcta atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa
     8221 caaaagtaaa catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc
     8281 tggtttttat gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat
     8341 gcaatcagtt agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa
     8401 attaccagaa gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac
     8461 taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt
     8521 cagtcccgat ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga
     8581 acgttttggt gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg
     8641 agctgggaaa ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa
     8701 gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt
     8761 tgaccatgga gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt
     8821 ttgttttcat gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt
     8881 tgcaaccaat caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa
     8941 gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa
     9001 agaaaacgaa cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc
     9061 aacagacgcg ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt
     9121 cttattagat gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga
     9181 gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa
     9241 aaaagcatcc acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa
     9301 agcttacgat ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt
     9361 ttataaagtg aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg
     9421 ggactggctg atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta
     9481 cccacattac aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga
     9541 agatggcatt attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt
     9601 aagcaaggcg ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga
     9661 tcttttctca tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga
     9721 aacccagaag cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa
     9781 aacgattatt taataagtga taatggagga agaaaagatg aataaagagg aattacaaat
     9841 gcttggtttt gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt
     9901 gaaagaagta agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga
     9961 aaatttaacc cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa
    10021 agagatggag atgggattca tttttatcca tgggcaggat catttaatga ccactttatt
    10081 gctgagagat ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa
    10141 gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat
    10201 ctcacgtaat agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat
    10261 tttattttca agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg
    10321 gcctgaatct gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg
    10381 gttactcgtt tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc
    10441 gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt
    10501 gttaagcgtc tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact
    10561 actggcttcc tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa
    10621 agatattacc attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga
    10681 tatttttcct ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca
    10741 aatcttccaa atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc
    10801 agctgacggg tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt
    10861 cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt
    10921 ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg
    10981 tttagggaat tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat
    11041 cttcctactc tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc
    11101 agtaagtttc gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta
    11161 tttttttatt ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt
    11221 cgatgtattg ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat
    11281 cggtttggtt ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat
    11341 cgtcgttgac ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga
    11401 gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg
    11461 tgaggaagta cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt
    11521 gttggtactt tgtgctggcg cagggacaag tgcgatgtta gctaatgctt taaaaaaagg
    11581 tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga
    11641 aatgctgcag gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga
    11701 tatcaaagaa gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata
    11761 tatcggatta acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata
    11821 aaggaggagc cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg
    11881 tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc
    11941 gcggagcaga tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag
    12001 acgatatttc aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac
    12061 tgcgtttgtg gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg
    12121 atctggaaac gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc
    12181 tggattttca ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat
    12241 ggcaaggtct gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc
    12301 tgtcggaaat gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga
    12361 atagcggaat gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc
    12421 gattggctgc ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa
    12481 ctccagtaat gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg
    12541 atgaaatcac gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt
    12601 ggcacggttc aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta
    12661 aagaagtgat cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc
    12721 caaatgcttt tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac
    12781 aagcagagta tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg
    12841 cgggcttctt ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag
    12901 caggtatgaa ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga
    12961 tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc
    13021 aaataaatct agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca
    13081 attggcaaac ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca
    13141 ctttttttgg ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt
    13201 atcaaaatgt tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg
    13261 ggggagagtt tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata
    13321 ctgcggttgt taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa
    13381 caggtgtttg tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg
    13441 attttgtttc attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc
    13501 ccttcagcca catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa
    13561 aaataaccag aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat
    13621 ctacactttg tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt
    13681 gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt
    13741 cgtgaatatg atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt
    13801 ttcgagaact tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt
    13861 ggtagggaac catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga
    13921 tttataggga ttccccctcc atattatgtt ggctctttat tcccactctg ttttcaacgt
    13981 aaagtgtata aaacagaatg cgatactatc aagggagtta aaacaatata cagaacagta
    14041 aaacactata aataaaaata aaagtgcaat aaaagtgcaa tttttgcacc taatcatact
    14101 ctgtttatat atgaaaacta gattctgaat ccaatatttg gttaacgaga ggcgttcgaa
    14161 tttgaacgtc ttttatatat tactttcaaa taaatatgag gtagaatact atctattttc
    14221 tttttaaccc aattgttata atggagagtg taaaatattt tgtgtaaata gaaaaaagga
    14281 agtcccttct gtagaataga gttaccacaa cacattcaca gaagagagga cttccctatg
    14341 aacgatttta ctacagaaat tctaaagact ctagcgaaca aaggcgattt gaatgaatta
    14401 ttccgtgtcc atttggagaa agcagtcaat acgcttctca aaacggagtt aacggctttc
    14461 ctagattacg aaaagtatga tcgcattggt tttaacacgg gtaattctcg taacggctcc
    14521 tatgaccgta cggtcaagac cgagtatggg gaacttcatc tccagattcc gcgcgaccgc
    14581 aatggtgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga cacgttagag
    14641 gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt
    14701 gagaaaatgt atgggcatta ctacacgccc caaaccatgt ccaatataac aaaatcattt
    14761 acagaagagg taacggcgtt taatgggcgg gagcttcatg accgttatgc tgctatttat
    14821 atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg ccaaggaagc tattcatatc
    14881 gcagttggca ttcgcccgga cggatcaaag gaagtattga gctatgcgat tgcaccgact
    14941 gaatccatca cgatttggga ggaaatttta ttggaccttc aagagcgcgg tttgaaaaat
    15001 gtcctcctgt tcatcacgga tggcttaaag gggatggtag gagcgattag tcggttctat
    15061 cccaaagctc gttttcaaca ttgttgtgta cacgtttccc gtaatatcag tcataaagtg
    15121 cgtgtcgatg atcgtaagga agtctgtgat gattttaaaa tggtgtatca agcctcatct
    15181 aaagaggtgg cgttggaagc acgtggtact tttgcggaaa aatggaaaac cagctatcca
    15241 aaagtggttg aatcgattct ttcgaacgat cacttgctca ctttctatga tttccccttg
    15301 gccatacgca agagtattta ttctacgaac ttgattgaat cctttaataa acaaatcaag
    15361 aaatacagcc accgcaagga acagttccaa aatgaagagt cgatggaacg tttcttagtc
    15421 tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca gtcataaagg tttccaacag
    15481 gccgaaggcg agctcgaaca aatgctaagc caactgattg agaattagaa acaatctgca
    15541 gtagggaaga atcatttaca caaaattatt gacgctccct tataatgaga acttttacta
    15601 acataattta tgataaactt actatataat catattaatt aaatagttag gagatctgct
    15661 tatgtttata tctactcttc aaattcgaaa ttttagaaat tatgaatccg aaatctttcg
    15721 attcgataat gagacaaata ctattatagg tgagaatgat tcagggaaaa ccaatgctct
    15781 tactgcattg agaatcttat tagatgatag ttattactat agtagtaaaa cactaaaaga
    15841 atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg cattggatta ttatctctgc
    15901 gacatttgaa ggaatcagcg aagaagaatt tgacaatgag atttgtgctt ccatttcttt
    15961 aaattctgaa agtcaaacaa tcttagaaga attgatttca aatgcagata aaggtgtagg
    16021 tagtatttct ctatttatta gacctaacaa agctattaga aagcaattat ttgatatttc
    16081 cactgaatta gactctcatc aatttaacga atttagaaat tctattcgtc tatccgacta
    16141 tgaattttac tacacatcta aatcaaatct aaatttctgt atcaatgaaa attatgataa
    16201 attggtagga agactcaatg acaatatagc tagtaatcct gaagaagacg atgaagctct
    16261 gttgggctca agaattgaca tgtcagatat tttcaagcat atttcggttg tttatgttga
    16321 cgctcttcga gatgttctta gagaaatgaa aaacaatcga aacccagtca aaagaatcat
    16381 ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag tcattaaaaa caattataca
    16441 gcaattaaat gagactatta ccagtgttcc tgaaattagg aaaattggcg aaaatattaa
    16501 tcggcaacta aatagcataa ttggttcagt gtattctcct aatcttcttc tttcctcaac
    16561 tatgtcagat gatatgggtt ctttagcaaa atttctttca atgaagccag aacaaaatat
    16621 cgacttagac ttactagggt taggacatct aaatatgatt tatttagctt tgaaaatagt
    16681 cgaatttgaa gcgtgtcgtt caagagaact acttaatatt atgttgattg aagaaccaga
    16741 agctcatatt catcatcata ttcaaaaaac tctgttcgaa ggattaaatc ttcaaaaaaa
    16801 ctatactcag attctcatga ccacacattc tgtacattta gctgaagtgt ctgaaatatc
    16861 tcggatgaat gtattaaaat cgtttaatgg caaatctatc gttatgaatc cagcaaataa
    16921 tctgaactca tttgctaaag aaaagttaaa aaagaacaat ctaaatctaa taaacgcagt
    16981 agagagatat ttagatgtta aaagaaatgg attgcttttt tctaaaggtg ttatcttagt
    17041 tgaaggagat gctgaagaaa tacttattcc tcaaatagta aaaaaattat ttggtataaa
    17101 cttagacgaa cttggaatag gtttgattaa tgttggtagt acttcgtttg aatatatcgc
    17161 tagcttgttt gacgatgata gaatacagag aaagtgtgct attgtaactg atttagatga
    17221 acaggcaatc ccatcagaac atcgattgta taaaagcact gcacaagaga aaggtcaagc
    17281 aagaaaagaa aaattatcct ccctttttaa tgaaaacaat tgggttaatt gtttttatgc
    17341 taatacaaca tttgaaattg agtttttaga tgtgaataaa gaaaatattc taacttatgt
    17401 cagtccaatt atagatgacg cattcaaaga taaaaaaaca ataactgctt ataaagaaga
    17461 aatacaaagt gaacaatatt tagaaagaaa tttagcgatg ttaaaactag ccaatcatgt
    17521 tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt gactttttaa tcaaaattcc
    17581 taagtatatt cttaatgcta ttgcttttgc atgtcaggaa atagtagatc tctcaattta
    17641 taaaaaaatt ctactccata caatcgactt gtacgaggaa actacagaat tgtcagcttt
    17701 atcagctagt ttaaaaacaa gtgatacagc tgatttgaat atagcaataa tttctttatt
    17761 agagtacttt ggtaaagatg aggatgttgt gtatctaatc aatcagatag aaaattacac
    17821 aaaaatactt tatgctgagg tttcaaaatg acaaatatct tcgaaaaaat cgtaaatgat
    17881 ttaaatttag aacaaaaaga tgttgtggaa agtgaagata acctttatgt aaatgcatgt
    17941 cctgggagtg ggaaaacaag ggtacttaca aggaaaattg cttaccaagc aattaaacac
    18001 gatggatcac taaaaagaat aattgctatc acatacacta atagagctgc tgaagaaatt
    18061 aaggaacgcc taagtttact tagtatagat gatgatatta atatttgggt ggggacaata
    18121 catcagtttt gtcttgaatt tattatttat ccttttaaaa tgaatctctc aagagtatca
    18181 aaaggattta caattattga tgattatact caaagagctt acgtcagcca aattaataat
    18241 atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa ttgatttgtc actttctcat
    18301 gattttaaaa ttaatgaagt tcagtatact caagtttctg aaatgtatca cactatgttg
    18361 gaaaaaaata aagaaattga ttttgatatg attttactag tagcgtataa aattttaact
    18421 gtaaacagta acgttgcacg taatattgct agtaatataa gggcgattta tgtggatgaa
    18481 tttcaagata ctagagaatt acaatacaat gtaatagcta aattacttca aaacaaccct
    18541 caaattcagt caatgtttgt tggagatatt gaccaagcaa tttatggatc attagatggt
    18601 atagctaaat cagttgaaga actagaaatg ctaactagtc acacttttca atcaaagacg
    18661 ctacatggct gttacaggtc taatcagaga ttagtagact tctactctaa ttttcagagt
    18721 tgtccttacg agattgaaag cagagggaat aataaaaacg accgtggttt tataagttat
    18781 gattgcaaag ttactaagga ggatttaccc aacataataa aaaatataat aaaggaaaag
    18841 attgaaagcg gcattcatga aaaagatatt tgtgtacttg ctcctcaaca ctatcctctg
    18901 ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt gcaattttga tgctataaat
    18961 atatcaccaa ttaaggtaaa taatcatagt ttatttttca agttagcaat tttgtacttt
    19021 actgaatcag gtgaaaaggt tcgaagaaga aagctaattg caaacgatat tttaattatc
    19081 ttaattgtca aattaagtta gacagatcat caatacttac gtaactcaaa aatacttcca
    19141 aagg
//
LOCUS       DACDCV010000052        18915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDCV010000052 DACDCV010000000
VERSION     DACDCV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.052"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..562
                     /locus_tag="IV041_001735"
     CDS             <1..562
                     /locus_tag="IV041_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8382509.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="IV041_001736"
     CDS             762..1076
                     /locus_tag="IV041_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8382510.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="IV041_001737"
     CDS             1097..1474
                     /locus_tag="IV041_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8382511.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="IV041_001738"
     CDS             1484..2257
                     /locus_tag="IV041_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382512.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="IV041_001739"
     CDS             2279..3682
                     /locus_tag="IV041_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8382513.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="IV041_001740"
     CDS             3864..5048
                     /locus_tag="IV041_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8382514.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="IV041_001741"
     CDS             5045..5335
                     /locus_tag="IV041_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382515.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="IV041_001742"
     CDS             5332..5553
                     /locus_tag="IV041_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382516.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="IV041_001743"
     CDS             5595..6374
                     /locus_tag="IV041_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8382517.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="IV041_001744"
     CDS             6441..6935
                     /locus_tag="IV041_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8382518.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="IV041_001745"
     CDS             6991..7629
                     /locus_tag="IV041_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382519.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="IV041_001746"
     CDS             7701..8096
                     /locus_tag="IV041_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8382520.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="IV041_001747"
     CDS             8080..10533
                     /locus_tag="IV041_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8382521.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="IV041_001748"
     CDS             10530..12557
                     /locus_tag="IV041_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382522.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="IV041_001749"
     CDS             12554..13576
                     /locus_tag="IV041_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8382523.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="IV041_001750"
     CDS             13593..14522
                     /locus_tag="IV041_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8382524.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="IV041_001751"
     CDS             14767..14883
                     /locus_tag="IV041_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8382525.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="IV041_001752"
     CDS             14899..15702
                     /locus_tag="IV041_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8382526.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15877..16173
                     /locus_tag="IV041_001753"
     CDS             15877..16173
                     /locus_tag="IV041_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382527.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="IV041_001754"
     CDS             16180..18132
                     /locus_tag="IV041_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8382528.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV041_001755"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV041_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8382529.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         6488 a         3130 c         3664 g         5633 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatga
//
LOCUS       DACDCV010000053        18805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDCV010000053 DACDCV010000000
VERSION     DACDCV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.053"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IV041_001756"
     CDS             256..531
                     /locus_tag="IV041_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382530.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IV041_001757"
     CDS             544..2616
                     /locus_tag="IV041_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8382531.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IV041_001758"
     CDS             2781..4667
                     /locus_tag="IV041_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8382532.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IV041_001759"
     CDS             4679..5626
                     /locus_tag="IV041_001759"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8382533.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IV041_001760"
     CDS             5646..6173
                     /locus_tag="IV041_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8382534.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IV041_001761"
     CDS             complement(6236..6889)
                     /locus_tag="IV041_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8382535.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IV041_001762"
     CDS             7021..7863
                     /locus_tag="IV041_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8382536.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IV041_001763"
     CDS             8021..8917
                     /locus_tag="IV041_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8382537.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IV041_001764"
     CDS             8920..9633
                     /locus_tag="IV041_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8382538.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IV041_001765"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IV041_001765"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8382539.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IV041_001766"
     CDS             10168..10395
                     /locus_tag="IV041_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8382540.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IV041_001767"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IV041_001767"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8382541.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IV041_001768"
     CDS             complement(11181..11723)
                     /locus_tag="IV041_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8382542.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IV041_001769"
     CDS             complement(11960..12358)
                     /locus_tag="IV041_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8382543.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IV041_001770"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IV041_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8382544.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IV041_001771"
     CDS             13471..14238
                     /locus_tag="IV041_001771"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8382545.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IV041_001772"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IV041_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8382546.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IV041_001773"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IV041_001773"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8382547.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IV041_001774"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IV041_001774"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8382548.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6540 a         3243 c         3913 g         5109 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt ctggctcttt gtcaataagg actgatgagt tgtgcaaaat
    18781 caaatctgag tcagaatgaa ccaca
//
LOCUS       DACDCV010000054        18366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDCV010000054 DACDCV010000000
VERSION     DACDCV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.054"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(217..651)
                     /locus_tag="IV041_001775"
     CDS             complement(217..651)
                     /locus_tag="IV041_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382549.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(883..1593)
                     /locus_tag="IV041_001776"
     CDS             complement(883..1593)
                     /locus_tag="IV041_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8382550.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1583..2443)
                     /locus_tag="IV041_001777"
     CDS             complement(1583..2443)
                     /locus_tag="IV041_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8382551.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2448..3086)
                     /locus_tag="IV041_001778"
     CDS             complement(2448..3086)
                     /locus_tag="IV041_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8382552.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3101..3400)
                     /locus_tag="IV041_001779"
     CDS             complement(3101..3400)
                     /locus_tag="IV041_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8382553.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3443..4927)
                     /locus_tag="IV041_001780"
     CDS             complement(3443..4927)
                     /locus_tag="IV041_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8382554.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4947..5408)
                     /locus_tag="IV041_001781"
     CDS             complement(4947..5408)
                     /locus_tag="IV041_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8382555.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5426..6493)
                     /gene="ulaG"
                     /locus_tag="IV041_001782"
     CDS             complement(5426..6493)
                     /gene="ulaG"
                     /locus_tag="IV041_001782"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8382556.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6711..7481
                     /locus_tag="IV041_001783"
     CDS             6711..7481
                     /locus_tag="IV041_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8382557.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7754..8581)
                     /locus_tag="IV041_001784"
     CDS             complement(7754..8581)
                     /locus_tag="IV041_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382558.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9149..10384
                     /locus_tag="IV041_001785"
     CDS             9149..10384
                     /locus_tag="IV041_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8382559.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11383..11643)
                     /locus_tag="IV041_001786"
     CDS             complement(11383..11643)
                     /locus_tag="IV041_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8382560.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11670..12971)
                     /locus_tag="IV041_001787"
     CDS             complement(11670..12971)
                     /locus_tag="IV041_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382561.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13263..13721)
                     /locus_tag="IV041_001788"
     CDS             complement(13263..13721)
                     /locus_tag="IV041_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8382562.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(13734..15059)
                     /locus_tag="IV041_001789"
     CDS             complement(13734..15059)
                     /locus_tag="IV041_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8382563.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15076..16488)
                     /locus_tag="IV041_001790"
     CDS             complement(15076..16488)
                     /locus_tag="IV041_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8382564.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(16505..17269)
                     /locus_tag="IV041_001791"
     CDS             complement(16505..17269)
                     /locus_tag="IV041_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8382565.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(17373..18248)
                     /locus_tag="IV041_001792"
     CDS             complement(17373..18248)
                     /locus_tag="IV041_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8382566.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         5763 a         3842 c         2812 g         5949 t
ORIGIN      
        1 aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa atcatgtgtt cattttttat
       61 attagttcga atttgcaaaa aattgtattt gataaagagg tgagtaaaaa aataaaatag
      121 ctgagcattg gaggatagat tgagtgatac taagtctatc ctcttgtttt tctaaagaac
      181 taagctagaa ttttttcttt tgtacgttat tatatgttat ttgtcattac caaaaagcac
      241 cttttttctt ctagtatcta tgacataaga aataccaccc atttccttta cttgaacaaa
      301 tcctactgat tcataaaatt tttttgtttt ctcagtatca tctgtcaata attcgatttg
      361 gactggtcca taactagtga tcttctccaa agcttcattc aaaagcactt tgcctattcc
      421 ttggcgctga tacctcggat gaatgaggat atcttgaata aatgcgatcg tcgcaaaatc
      481 agtgatccaa cgaacgattc cgataatctt cccttcccca ttacgcttta tcagagattc
      541 agattgttca aatgctctag ctaaccttgc agtgtctttt gtgtaattcg tccaaccaac
      601 gctcttatat aattcagaaa tctcatcgat cataagggaa tcaatattca tttttttctt
      661 cctttcttat acaattctcc ctctaataat acagttcttt gactatttaa tctatcacat
      721 ataactctcc tgtaaagatt ctctcgattc cttaaaattc ctctttcctt ttagaatgca
      781 tactttttga taaataaaaa ggttgtgata aattttcttc acaacctctg tttctctcta
      841 tcaagtgtta tcttttaatt ttctaccctt accctagcat ctctattgcc cgtagtatgc
      901 atactttcca tgtttgcgaa aataatgttt atctagtaaa tattgcggga tttcacatat
      961 attggggttg atcagctcgt tttctttggc catcagacaa atttcatcta agatcaaact
     1021 gttttctact gctttcatag gtgaatcccc ccatgtgaac ggtccatgtc catagacaag
     1081 tactccaggg acttcgtttg gatctaattt tctttcctta aacgtctcga caatcacatt
     1141 ccctgtatga acttcgtaag cttctcttac ttcttctttc gttaattggc gtgtacaagg
     1201 aaccttacca taaaaagcat cagcatgagt cgtgccataa gctggcaaat cgcgtcctgc
     1261 ttgcgcccac atgacggagt gcgtggaatg agtatgagta atagcagtta tatcttcaaa
     1321 agtttgatac aaaacaacat gtgtggggag atctgaagat ggttttaagc tatcttcttc
     1381 tattatattt cccgacaaat ctgttaccac catttgatct gcttgcatgc attcatatct
     1441 gacaccgctt ggcttaatga cgatgactcc tagctcacgg tccacttcac tcacattgcc
     1501 ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga tttgcttcaa ggacacgttg
     1561 cttcattgct gcaatttttt cttcacaaga catcatagcc agcctccttc aatttagaac
     1621 ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc ttggcttttt tcactccaca
     1681 tttcaatcag aaatgttcca tcataatcta gacgtttcaa atgtcttaat aatccaagaa
     1741 aatcaacaca accgctacca aaaggaacat cacgaaactg gcctgaatat tcttttgtta
     1801 cagggtaagt atcttttaag tggatagcag tgatataatc gatccctttc tcaagttctc
     1861 gagccggatt attttccggc catgcggaca aattgcctag gtctggatat acttgtagaa
     1921 aaggtgaatg aatctgttct ttgatttcta aaaattttga aatggagttc ataaatggat
     1981 catccatgat ttccacggaa agaatgatgc catatttaga agcttccttt acgctttctt
     2041 ttattccttc gataaagtct tctctggaat tcattgattt ctcttcataa taaacatcgt
     2101 atccagctat ttggattact ttgacgctta gattatgcgc caaatggatt gcttttttca
     2161 ttagttgttt cgctgctttt ttcttttctg gatcagcaga accaaacgga aatcgccgat
     2221 gcgcacttaa acaaatcgaa tggatacgta caccggagct gaacatctct tctctcagct
     2281 cagctatctt ttcttcagac caatctaatc gtgctaaccg ctcatccgtc tcatcaatcg
     2341 acatctcgat aaaatcgaag tccatttctt ttacaaggga aaaacgttct ttccatgaaa
     2401 tatctttggg taacgctttt tcataaatcc caatttgagc catttcctta cccccagata
     2461 cgaatgatct catctttgaa gttttgtgcg gccttcatcg gatcatttgc tgctgtaata
     2521 ccacgaccag tgataaaggt atacacatcg atcccttcaa ataagtgtaa tgtgtgtgga
     2581 tttaatccac cagttacaga aacattaaag cctagttcaa tcagtttttt gatcttcgat
     2641 aaatcttttt caccccaagt ttcaccagat aacagcgcat cacggctttg atggtagatt
     2701 acctgccgga taccgatgtt atgccaatcc atggcttgtt caaaagtcca attgccgtag
     2761 agttctactt gcaattcacc tatttctttt tgtgctgcct ccatagttgg aatcgttgca
     2821 caacaaatga ctgtcatcca gtctgctcct gcttttgcaa cattactcgc cactgttcct
     2881 ccagcgtctg cacatttcgt atcagcgact agttttttat ctgggaacat actgcgaata
     2941 cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg tgcccacttc aataatatct
     3001 actacatcac caactttcat aacatctcct aatgcatgtt ctaagtcatt gtgatctaac
     3061 gcaatttgca aattcggcct gctcatctta gttccttcct ttacttgttt ttttggtacc
     3121 actttgtttc agcaaatttt tcactgattt ctttgactga catcacattt ttgataccga
     3181 taactgtaac tccttttttc tctgcctgag taaacatatt caaaaaattg actggcgtaa
     3241 aaactacatc gtattggcca gctgaacttt ttccctctga aatggcacaa tgatggattt
     3301 tggtaattgg tacgtttagt tctttcattg ctttttccac acttctcatc atcatcaagc
     3361 ttgttcctga tccatttgca cacgatacta aaattctcat cttaatccct ccattatgag
     3421 tgttgtctaa tcagattttc ttttatactc tctgcttttc tgcatatgtg tcatagtctt
     3481 ctacaatcaa aaaataacca tcaggatttt tccgatattg taactgcgga atagccaata
     3541 aaccaatgac aactacggca attccagcat aacctaggac tttcatgatt actgtaaaca
     3601 atggccaagc tgttgcccag tcgaacatcc cgatataacc accgtatgtt gacatcccaa
     3661 tccatgtagc gaataatgct gaaccgccta cttgtatgat tcctgaaaga aacgggaaca
     3721 ggcaagcggc tttgaatcca cctcggttat tggcataaac tgctataacg gcattatcaa
     3781 agaacaacgg aatgaaaccg gctatgacga tcgttggtga tttcaataaa atcagtaaac
     3841 caatcatcaa aaactgtccc aaagcaccaa acaagaaccc aatcgttacc gcatttggcg
     3901 aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac agctcctggt aaaatcgtgt
     3961 ttgagattcc ttggaaagat tgagttagtt catcgacgaa tgtccgaaca cctaattgca
     4021 agatcgccag atataccgca aaatttaaag aagtcgtcat aatgtagaat aagaaactct
     4081 gcccttcttg catgaattgc gcttgaatca aataatcttt gcctagtaca atcagaatga
     4141 tcccaaagaa aaatagcatg agtaacgaag tagcaaccat attctcattg aaaatcgaca
     4201 agaatcctgg taatttgata tcttcaattt ttttgtcttg cttgattgat ctatttttct
     4261 tttcatcccg ctttttcatc cactcagcta attttgcaaa tataaagatt ccgaacattt
     4321 gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc ctgcgtgatt cctactgtta
     4381 agttagaagc tactgtccaa taacaaccta aaatcagtcc cataaaaatc aatgcttgta
     4441 tcctacctag atcaggaaag caaaacagta aaatccaaaa agcggtagct gcttgttgaa
     4501 cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt atatttctga aaacggacta
     4561 ataagatatt taaaacaaat gcaatcagta ataaaatcat cacatctcca aaggtacggc
     4621 caaatgtctc cattaatcca gcatccacag cattttgtcc aaaataagga tctgtaacca
     4681 tggcatccaa attgaaccgt tcctttagtc caaccaagat tggccgaaaa ttattgacca
     4741 aaccaccaga gccaacagtc aaaataaagt aaccgaccgt tgcctttaag aatccagcga
     4801 tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa taacacgata aaaccaatca
     4861 ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt ccaaatgctt aacaaaaatt
     4921 cccccatctt ttttctcctc tatcaattaa ttatattctg ccatcacaac ttggtaatca
     4981 tcgatggagt tcgtagagat gagacgctca accaatccat ctgtcattaa taattcggat
     5041 aatttttgga tattttctag atgttcttct ggatcttttg ctgcgagtgt aaaaaacaaa
     5101 ctagccgttt tctcctcatt ctccccttca ctttcaaaac ggattggctg tttcaacttg
     5161 gtaaatgaaa tagctgttcc aaaaacacca tcactttttt ctgaagaatg cggcattgct
     5221 acttgaggtg cgatcacaat ataaggaccg tatttttcta cacaatggat gatctcctcc
     5281 acatacgttt catcaataat ttccttatcg atcaatacaa gacagctttc ggcgatagct
     5341 tcacgccaat ccgtgatttc tttatctggg taatgaatca attgatggtc aaaaaagtat
     5401 ttcagcaaga aactcccctt ctttcttata aaaacgacgg aaacggtgta tcattttcaa
     5461 tcgagaagac atcttcaaat cctcgataaa aattaaactc aagatcatct ttgtttgtcg
     5521 gatagatgaa tttcccgcct acttgccaga tataaggttt aaatccatac ttcaggcgat
     5581 cctttttgaa tttccaaatt tcagtgatct cttttggatc agccataaaa ttagaccaaa
     5641 tatcgtagtg gacaggaatc actacttttg cattcaatga ctccgccatg cgcagcatat
     5701 caacagaagt aactttgtct gttatccctc gtgggttttc accataggcc cctaaacaga
     5761 catcgatttt gtgttcatta ccatgtttgg caaataaatt ggagtaatgt gaatctcctg
     5821 catgataaag attccctcct gatgtttcga ataaatagtt tactgcaatt tgatccatct
     5881 cctgaggcat ttttcctttt aatacctctt ctggatcttc acaagtgacc aacgctgtcc
     5941 gatcaaacgc ttctaaagct actacattga tgtctttgat tttgactgca tcacctggct
     6001 ttactgtaat cgttttttct tcaggaattc cccatttttg ccacgtgcga accacttctt
     6061 ttggcccaat gaatgttgct tcaggacaat tttgatgaac tgctgcggct gtatggatat
     6121 ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc tacatttttt atcgcaaaag
     6181 gatcgattac gaaaggctga gtacgtaaat taggctgcat attttgaacg ccactcatac
     6241 gcatcatctg atggcctttt ttcatcatgc cattcccatg actacgtttt cccgtcccac
     6301 accatagatc acataaaata ttcgttcctt catgactttt cagccaaatt cccgtacagc
     6361 ccagccacca catagcaaaa gttccaggat ccactacttc tgtttcgatt tcttcattca
     6421 agtatgtgcc ccactcagga aatgtttcta ctaaccactt ttcttttgtt acttcattga
     6481 cagttttact catttgcttg ctccccccat atcgttgttc caacttacaa gtaaatatta
     6541 atgtgtatca gcatccatga aaaccctttt atatgaccgt ttgtttttta tatgattttc
     6601 tttcagtaat caatgattgt ttcagtataa taaagacaga tatgaatgtt taaatgaaca
     6661 actagtcaaa taaatccatg gatcaagatg tccctcagga ggcaaaagtc atgaaaaact
     6721 cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga actgcttcag cagactgaac
     6781 aactatcaac tttcgaatta gctgaatccc ttgatgtatc tattagtacg atacgaagag
     6841 atttaaattt attagaagaa aaaaacgaga tcatacgcaa ttacggttat tgttcttata
     6901 attacaaaaa ccagactgat tttgatcaat ctggtcctga aaggattaaa caagcgattg
     6961 ccagagaagc tagtaaatac attaacgatt atgatacgct gtttatcaat tctagctcga
     7021 ctgcgttgaa aacactggat tttcttcaat ctaaccattt gacagtcatt acaaacaacg
     7081 tgaaaatcaa ttactctccg cacaaagata attgtagtta tattttaact ggtggagaga
     7141 tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt aaatacgatt tcttcagtca
     7201 atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt agtacatggg gtcacaacaa
     7261 agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa caagacaatc aaatgtaaga
     7321 ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa gttcaagatt gctgatattt
     7381 cagtctttga ttatttgatc actgataaat tttgttctga aaaagtatta gaggagatca
     7441 gaaatacggg gataaaaacc attcaagtag attcatttta agaaccagga cgtaaatatg
     7501 taaataggat tatcaaaaaa atgaataaca aatttatcga taaatgaaaa acacgctacg
     7561 ctcaaattga taaaggaatc ccaagatgta tccatccaat cgataccatc ataatctatt
     7621 gcttctttta taaccgttat atgttgccta aataggaatc aaacaatatc gaaaaaagga
     7681 ggtatgacaa aagtcacacc tccttttttc gaataaacgg tattcaaatc agaacactga
     7741 tttcacaacc actttatttc aggtcgtttt caatcacctt taccgcgtaa tcatttccgt
     7801 tgaaacgcgc tatttttttt gcgtccatat atctttctaa ataatcatct tctcctaatt
     7861 gcttatggca agaagcaatt tcataaagca agtattccaa tgcataagtc gatttatgtt
     7921 tcttattata ctcaataccc ttctcacata attggattgc tttatcataa tcctcgattt
     7981 tacggtaata ggtggcgata tcataatata caatgcagac catcagatta tcaaaatcag
     8041 tctcactcaa tattttaagt gtacgctcca cataaacttt cgcaaaatct aattctcctt
     8101 ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa gtgatggata tctgtttcgg
     8161 tcgtttgttc caacacttga ttaaaataaa ataatgctgc atctggtttt ttcgcataaa
     8221 gatgatcaag cagtccttga taaaataaaa tttgatttaa aagatcagaa ttacttactt
     8281 tttcctgatc gatcgtttgt aaaattttat aagattcttg gtattttgat acagaattca
     8341 atgattgaat ctcatttatt tttttcctga ttttgtcttc ttctgtttct actaatatat
     8401 cggagatctc gacattcagc tttttgcaaa gcgcacttaa gatattgatg cctggcgaca
     8461 tattcttgtt ttcgatattg ctgacagttg cttgttcaca aatgccttct gctaattgta
     8521 cttgtgttaa cccctttttc tttcggtatt cttttagtaa tgctccatct actttaatca
     8581 aaacaatttc cttcccttta aaaaattatt tctcctttat atcatacaca aaaaaaagtt
     8641 ctaaacaacc aaaaatgttt agaacagaat aaaaatttta attttaaaat aatattcaaa
     8701 ttaaacaact ggcgtatctg ctccttgatt tctttttgtg ttatctgaat aattatcgtt
     8761 tacagttata acacatagtg aaaattgtca tggcgagaaa gtctaagaat atttttaaac
     8821 ataactattt ctttcttacg tgtgtataat gacgacagca cacctatcaa ttaatataat
     8881 tattttattt ttttatgttc tcatgtttat ttttaatgac acaacttctc gtatttatct
     8941 ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt ttttgaaaat tggagtttca
     9001 acttgattac aaaatttaaa tttagaagtt gtgaaaacag cgagagacag tttaatattc
     9061 aaaatataca taggctactc gaacgctgtg ttttttagta tactagtaag aaacgaaggg
     9121 atagactcat tccaataagg aggatttcat gaagacaatt ctttttgctc ctgcagtatt
     9181 caatttagct gagacaacac gaatgattga ggttgccaaa gctatgaaag accgttatta
     9241 ctgtgtattc tttggctttt ctaaaacttt tgctcctttg atcacagaaa gtggctttga
     9301 gtatcattta ctagcgccga tccttacgaa aaaacaagaa gaacaaatca taaaatttga
     9361 ccagttgcgt acacttaaaa acccttttac caaagaaatg gttgcctctc gtatcactag
     9421 tgaacagagt ctgattgcta agtttgagcc tcgactgatt gtgacaggtt cgaatgtgac
     9481 aattttttta tcggcccgca ataaaaagat tcctttagta tatgttaaac cctatgcttt
     9541 gagtgaacct ttttttcaat cagagcatgc gctgatgccc cacgagttac gtttcttcgg
     9601 ccctttaagc aatccccttt ggcactggac aaaaaagcgt atagtaaata cccgatggat
     9661 ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt cctttaccaa aaacaagttt
     9721 acaaatgatg gatgctgatc taaatctcat cacaacgcct aaactatttt tggaagaaga
     9781 acagctgccg gaaaattatc gaatcgttgg tccaattttt gctaagctca gtacttcctt
     9841 gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa gaaagaaaac gtattatcta
     9901 tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc aaaattttgt tcttcttaca
     9961 aaaacaaccg gatctagcgg ttattgcacc tattgactat ctgcttgatg acattcaatc
    10021 tgactcagaa gatcttttaa taacagaata tctgtctagt catcttttgc atgaattcat
    10081 tgatttttct tttatccacg gcggggaagg gaccgtccaa actgcctgtg aatccggcaa
    10141 accgtttatc ggtgtgggga tgcaatttga gcaaagctgt aatattgact attgtgtaaa
    10201 atacggaaac gcaatcgccc tcacaaaacc agtgactgaa agaaaactgc agattgctct
    10261 gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc acgctgcaag ccgtttttcc
    10321 aataaatggt ccacaacaag caattcaaga aatcgaaacc ttcctgagca accacccaat
    10381 atagtttcaa gtagaaaaag cttttttaat agagactcat cagtaatttc gctattcgag
    10441 acccagacag tgaaaataca aaaaaagtaa atagtggatt gaaaagttag agttacttaa
    10501 attacggtgt ctaaaaaaca caaaatcatc cttaaaataa aagatctgac gctatccaaa
    10561 tgagcatagg tagcgtctgc catttattct tagtcaaatt ctttgacacc tttctctggt
    10621 tggatcatct caacgtttct tcaaaagaaa gaccataata attaggcaaa tacattttct
    10681 taaaatgtgt catatagatc tagaagcatc gctttgtaga gatttgtgcc gctagtcccc
    10741 tcaacaatta ctaaatcaaa tgtaggattt accactttat ctttgataat caacctccct
    10801 cttctgttga gacaatgatc tcgcaatccg atgcatactc ctatataaac tgccagcaat
    10861 ccccacatat tgagtagatc atgtcagaat gttctatgtc gtcagcaacg atacactaaa
    10921 agtcacgttc accatgactg attgctattc ccagcacaat tatttctata cacgtttcat
    10981 gatttgaata aacactcaca tcatacatat taccattcat acaccatact gcagccccta
    11041 ctatatggtt gtaagctccc aaatctatat ttgacttgat cactttggta gtagatctac
    11101 taattctaaa tccattgctg ataaaatttc cattttttcc tccttctttc tgccaaaaca
    11161 gttaaaacaa taataatctt tatgtaaaaa agggctgtga caaaagcctt gtcacagtcc
    11221 catattccga ataaacggtg gtcaaaagct tgcatctgcg gtaataagtt caaccaaacg
    11281 aaaaataagc caacatttca caaaaattta agaagcaatt ttcgtaaatt tcttcttatt
    11341 tcttgaagct aaccacttct gttacaacca cttttagaac atttaaagct tcgatgtgac
    11401 aaaccatcta tgctgctcga tcataaaatt gatagcaaaa attccacaaa gtacatatgt
    11461 cccccatgaa aacaaaacat tcatatgcgg tacaatgatc tttggaagaa tcacgatcaa
    11521 aaatgatgcc agtgcccaaa tccatcgatt gatttgaata tgatatgtag ctagcatatt
    11581 caatataagg tgactagccg ttaataaaaa gataaaaaca aaaaataaaa tcagccaata
    11641 cattgtttcc caccttctaa aaaccaaact tagacagtct ggacttcgtt ttctccacga
    11701 ctttgttcat ctgacagcat ctttttatca taagctttta caaatggata atagataata
    11761 aatgctatca atgtgttgat catgatcaag ataacaacac gccagtcatt tcctgctgaa
    11821 agataaccgc cgattggagc tggtaatgtc catgttggca aaacagtaaa tctattgacc
    11881 atttctaatt tgactgcaaa ataactgata atagtcaaaa ctaaaggaac taagttgaac
    11941 gggatgaaca tgtaaggatt caacatcata ggagctccaa aaattatggg ctcattgata
    12001 ttgaagatac caggaataat agaaaaacga ccaacctctt tcaagaattt tgatttacag
    12061 aagaatataa acaaaacaga taccacgatc gttgctcctg caccaccaat tgttaccgtc
    12121 cattgataaa actgttcagg agcgatatag ggtaattggg tggcagctgt accatctgcc
    12181 aatgcctttg cattctcatc caacatgatc agccacattg gacgaacaac agcccctaca
    12241 atactcatgc catggatacc aaatgaccac aacaaatgga tcaaaaacat tggtagtaaa
    12301 acaccaagca aattattccc taaaatattg gttaaaggct cgaataacga taataaggca
    12361 gcgtttacat caaagcccgc gacgcttcga atcagccaag taattgttaa aatgactgca
    12421 cctggaatca atgcctcaaa actacgggca acagcaggtg gcacttgttc tggcattttg
    12481 atagtaatat ttcgattttt aaagaaacga tatacttcca ctgcaaaaat catagataag
    12541 atccccgcaa acatcccaga tgctcccaaa gaactcatag gtaagaccca gccaagaggt
    12601 gcctcttcag gagttaaaac gtttacagga acttgtaaca taaagaacgt tcctaaagat
    12661 aatataccac ctgtcacact atctaatcca tatgatttag ctagatccga acccataaca
    12721 aaagttgcat aaatagccat caatcccatc gtcatcctaa agggtaaaac aatcgttgcc
    12781 gaataagggg caataaattc attccatcca ggaaacggta aattgataaa gaccataaaa
    12841 aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc cattacgaat tgctaataaa
    12901 tgacgctgtg taccaatttt tgccattggt gggacaagtt tctcttcgac ccatttcatc
    12961 agcttttcca ctaaaaccac tccttttcaa taaaatatga accattcatt gcatgacaac
    13021 gcaaagaatg ttcataacta attatataat taagaaattt tatttcaata tattctctta
    13081 aataatcaaa tttattttta agaatcctga aaataagttt tctaaatcat aggagcaaca
    13141 aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc tagtgattga aacatatcat
    13201 ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt tttcatcgta
    13261 cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt
    13321 ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac
    13381 ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc
    13441 cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt
    13501 tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt
    13561 attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct
    13621 atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta
    13681 gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt
    13741 aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact
    13801 ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct
    13861 tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat caatccttta
    13921 tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc accaacatat
    13981 gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg
    14041 attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat
    14101 tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta
    14161 tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac
    14221 tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc
    14281 gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa
    14341 gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata
    14401 atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag
    14461 tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca
    14521 atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac attcggatat
    14581 ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct
    14641 ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc
    14701 ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga
    14761 atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa
    14821 tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa
    14881 ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca
    14941 atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga
    15001 acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc
    15061 atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac
    15121 ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc
    15181 taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg
    15241 tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt
    15301 tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac
    15361 ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat
    15421 caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa
    15481 aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt
    15541 aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc
    15601 atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat
    15661 atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt
    15721 ttctttataa gctgctttat agatagctag acatatagct ggtataccaa acatcattac
    15781 tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat
    15841 ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata
    15901 cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat
    15961 ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg
    16021 gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat
    16081 agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt
    16141 aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat
    16201 attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc
    16261 agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc
    16321 aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt
    16381 cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg
    16441 aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc
    16501 tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc actttttgag
    16561 caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac
    16621 catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt
    16681 ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt
    16741 ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa
    16801 cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa
    16861 caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg
    16921 gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat
    16981 ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga
    17041 taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta
    17101 cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg
    17161 ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca
    17221 caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct
    17281 tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt
    17341 tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc
    17401 aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta
    17461 atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata
    17521 ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc
    17581 attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga
    17641 tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct
    17701 cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg
    17761 gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga
    17821 taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt
    17881 tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag
    17941 gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga
    18001 atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat
    18061 ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc
    18121 cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt
    18181 ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac
    18241 tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag
    18301 aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag
    18361 tttaac
//
LOCUS       DACDCV010000055        18126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDCV010000055 DACDCV010000000
VERSION     DACDCV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.055"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IV041_001793"
     CDS             complement(27..725)
                     /locus_tag="IV041_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8382567.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IV041_001794"
     CDS             complement(718..2070)
                     /locus_tag="IV041_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8382568.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IV041_001795"
     CDS             complement(2293..3693)
                     /locus_tag="IV041_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8382569.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IV041_001796"
     CDS             3854..4591
                     /locus_tag="IV041_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8382570.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IV041_001797"
     CDS             4716..5195
                     /locus_tag="IV041_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8382571.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IV041_001798"
     CDS             5192..5959
                     /locus_tag="IV041_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382572.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IV041_001799"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IV041_001799"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8382573.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IV041_001800"
     CDS             complement(6862..7464)
                     /locus_tag="IV041_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8382574.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IV041_001801"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IV041_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IV041_001802"
     CDS             8145..8729
                     /locus_tag="IV041_001802"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8382575.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IV041_001803"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IV041_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8382576.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IV041_001804"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IV041_001804"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8382577.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IV041_001805"
     CDS             10630..10809
                     /locus_tag="IV041_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382578.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IV041_001806"
     CDS             10831..11856
                     /locus_tag="IV041_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8382579.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IV041_001807"
     CDS             12031..13275
                     /locus_tag="IV041_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8382580.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IV041_001808"
     CDS             13428..14375
                     /locus_tag="IV041_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8382581.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IV041_001809"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IV041_001809"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8382582.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IV041_001810"
     CDS             complement(15277..15519)
                     /locus_tag="IV041_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382583.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IV041_001811"
     tRNA            15640..15712
                     /locus_tag="IV041_001811"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IV041_001812"
     CDS             complement(15831..16469)
                     /locus_tag="IV041_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8382584.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IV041_001813"
     CDS             complement(16592..17224)
                     /locus_tag="IV041_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8382585.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IV041_001814"
     CDS             17575..17874
                     /locus_tag="IV041_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8382586.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6214 a         3162 c         3571 g         5179 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatat
//
LOCUS       DACDCV010000056        17954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDCV010000056 DACDCV010000000
VERSION     DACDCV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.056"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..206)
                     /locus_tag="IV041_001815"
     CDS             complement(<1..206)
                     /locus_tag="IV041_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382587.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEG"
     gene            282..354
                     /locus_tag="IV041_001816"
     tRNA            282..354
                     /locus_tag="IV041_001816"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:315..317,aa:Lys,seq:ctt)
     gene            516..737
                     /locus_tag="IV041_001817"
     CDS             516..737
                     /locus_tag="IV041_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382588.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1061..1306)
                     /locus_tag="IV041_001818"
     CDS             complement(1061..1306)
                     /locus_tag="IV041_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382589.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1300..1701)
                     /locus_tag="IV041_001819"
     CDS             complement(1300..1701)
                     /locus_tag="IV041_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8382590.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1873..3681)
                     /gene="uidA"
                     /locus_tag="IV041_001820"
     CDS             complement(1873..3681)
                     /gene="uidA"
                     /locus_tag="IV041_001820"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8382591.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3709..5475)
                     /locus_tag="IV041_001821"
     CDS             complement(3709..5475)
                     /locus_tag="IV041_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8382592.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(5488..6282)
                     /locus_tag="IV041_001822"
     CDS             complement(5488..6282)
                     /locus_tag="IV041_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8382593.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(6293..7669)
                     /locus_tag="IV041_001823"
     CDS             complement(6293..7669)
                     /locus_tag="IV041_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8382594.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYVKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(7798..8445)
                     /locus_tag="IV041_001824"
     CDS             complement(7798..8445)
                     /locus_tag="IV041_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8382595.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(8529..9956)
                     /gene="uxaC"
                     /locus_tag="IV041_001825"
     CDS             complement(8529..9956)
                     /gene="uxaC"
                     /locus_tag="IV041_001825"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8382596.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(10030..11109)
                     /gene="uxuA"
                     /locus_tag="IV041_001826"
     CDS             complement(10030..11109)
                     /gene="uxuA"
                     /locus_tag="IV041_001826"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8382597.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11112..12743)
                     /locus_tag="IV041_001827"
     CDS             complement(11112..12743)
                     /locus_tag="IV041_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8382598.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            13060..13731
                     /locus_tag="IV041_001828"
     CDS             13060..13731
                     /locus_tag="IV041_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8382599.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(14567..15523)
                     /locus_tag="IV041_001829"
     CDS             complement(14567..15523)
                     /locus_tag="IV041_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382600.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(15632..15877)
                     /locus_tag="IV041_001830"
                     /pseudo
     CDS             complement(15632..15877)
                     /locus_tag="IV041_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16022..16444)
                     /locus_tag="IV041_001831"
     CDS             complement(16022..16444)
                     /locus_tag="IV041_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382601.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(16583..17013)
                     /locus_tag="IV041_001832"
                     /pseudo
     CDS             complement(16583..17013)
                     /locus_tag="IV041_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(17329..17592)
                     /locus_tag="IV041_001833"
     CDS             complement(17329..17592)
                     /locus_tag="IV041_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382602.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         5667 a         3543 c         2466 g         6278 t
ORIGIN      
        1 ccttcatctt gtattttcaa tatatactca atgacagctt ttttaggtat tttgtacctt
       61 gctccaattt ttatattttc tattgatcta tctttcagca aattataagc tttatttttt
      121 ccaatattta acattttagt taaatcagta attttcaata tcgatggata actttcaaat
      181 attttttcat aatcttgaaa atccataaaa ttcactctca attctgataa taagtattgc
      241 acttttttat tttaggtgtt agaataacaa agtattaatt tgggctgtta gctcagttgg
      301 tagagcagat gactcttaat catcgggtcg cgggttcgag cccctcacag cccattgggt
      361 gccaaaccct taaatacagt gtaaacgttg gtgcttagca tttagaaaac gtatatactg
      421 tatttttttg ttttcaaaag agactgtagt accccttttt ttcactaaaa gcccactgta
      481 gtcattgata tttttgataa gaaaggaaca taattatgcg taatgaaatt gctaatataa
      541 caataatccg agaaacttta caaaaccata ctgcaaatga gatctctaaa catacaggat
      601 taaatttgag tactataaaa aaactaaaaa gtggagaacg ttcaatagaa aaactaaatt
      661 tacatgatgc tatttgcctc actgaatttg gtcttaaaaa taatagaaaa aatgttgaaa
      721 tcaacatatg gaaataattc ttaatcagct gcttttgtca aaagcacata ggaatttcac
      781 ctctctccaa gtttacggcg agccttacgg aagtatcatt atcccaactt caaatcatcg
      841 ctttttattg actataaaaa ttattttaga atgtttatcg ctccttcttt acagtaagaa
      901 atcatggcgc aactaaaata tgctccttga agtgttaacg tactgataga atgtatttaa
      961 atttcaatga tcaataggtt ttctgtttaa aagaatctac taatccttta actattagaa
     1021 gattctttta aacagaataa gtggaaaggg gactttttag ttagttaatt ttaaatttta
     1081 agatcgcttc tatcaattta atttctagct gtcttagcat atactcatct acataggtaa
     1141 taaattgccc cgcctcatta aatccatttg tcaaacataa cttagctata taactatgat
     1201 aatgtttgag tatcatcttg attgaaattg gatcttcttc tagagcagat tggataatgg
     1261 atagaggaac taatggatac tgccgttttt tggggttgtc taccataagt cataccccat
     1321 ttttaattgt tcaactgcct tctttcgttg ataccagata ccacttgtac tcatattgag
     1381 gattttaccg atttcagtat cattaaaacc tgcaaaataa taaagtagga taacctttct
     1441 tcttatttcc gataaatgat caatggctgc cgataacttt tgatctgaaa ttaatatttc
     1501 cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggagt acttaccttc
     1561 atttgaattc gtcaatggtt cctcattatc tgttatgtta atgtttcgtt ttcgaataga
     1621 cttagtttcg tttcgtaata cttttttaca gtaggcatca aattgatgtt gtatagattt
     1681 ctcattgtaa ttttgttgca tgataaatat gctccttttt tcccctttcc actaatacaa
     1741 caatcagaaa gcgccttttt ttcgaaaaaa ctagaaaaaa atatccttct ttaaacagaa
     1801 aatcttttta taaaaaaaag gagttggcaa ttaagcagcc actccttttt tatattcctt
     1861 ataaaaggta aattattttt tataactgaa atttggtatt gatgaccatc gtttctttag
     1921 atagtgagca atcattttag gctttctatc tctagtaaag attcctttct tatttccttg
     1981 cactcttaaa atcccttgac ttgtttcaaa atctgcaaaa ttccacactt gttcaccaac
     2041 aaaattgtca catttatcta ttacttcgtg atttgcttcg taataaccca cttgatactc
     2101 ttctgtaaac attgttggta ctgtatcatg aagtccagct actgtatctg ctccatattc
     2161 tgtaaacatt attggcgtat cgggacatct ttccatccat ccatctagct cttttttcaa
     2221 cagatatttt gcatgtttta ggtctccacc tgaaacatac caaccataat agcgatttaa
     2281 acataaaaca tcgacaatat ctcctacttt acaaatatct ggtgttgact ttaagtaagt
     2341 aacgattgtt acaggtctct tctgagggtc aagttcttta gtcaattcaa ctaaaggttc
     2401 aaaatattct ttagcgcctt cagaatccga atctggttca tttgcaatag accacattac
     2461 gacactcgga tggttcttgt cgcgttctat caattcctta atcgccattt tgtgagcatt
     2521 ttctgtccgt atttctttcc aagtatcatg tttttctacg ttgtcatcta ataaagtagc
     2581 catgaaattc aaatgtaatc ctactgcagg tacttcatca ataactacaa taccttctcg
     2641 atctgctaag cgcataattt cttctgaata aggataatgt gaagtcctaa atgaattagc
     2701 accgatccat ttcattaaat taaaatcttt gatatttact gcttcattca aaccacgacc
     2761 attaatatac gtgtcctcat gcttcccaaa tcccttaaag taaaaaggtt tattattaat
     2821 caaaaacttc ccattcgaaa cctgaatact acgaatgcca aattcttctg tgtacgtatc
     2881 aatcacttta ttttcgttaa ttaaactaac tttcatacta tataagtaag catttagagg
     2941 ctcccaaagt gttgggttct taagttgaat cactccttct ttccccttag cttctgctat
     3001 aagttgagct tcttcgtcat agattgctat tagaatttct ccggtccatt ctgtatcgat
     3061 attaaattct acatttgcat ctgaatcaat tatttttgta ttaatagtga catcctttat
     3121 atagtctttc ggagttgtat acaatttaac gggtctatta atgcctgcat aattgaaaaa
     3181 gtcaaaatta accaaatttt ctttttttat gtctccatct tcctgtatcg tttctttcaa
     3241 aatccctaca ggtaaagtgc tatcgtcaat aacattgttt acagccactg taattctgtt
     3301 acttccatct tttataaact gattaatttc tgattcaaat ggcgtaaacc ctcctttgtg
     3361 ttgattaatc aattttccat tcaaaaatac ttttgcttca tgtgttgcag atccaaaacg
     3421 aagaactaat ctttcttcta ataatttttt aggaagaatc acttcttttt cataccacac
     3481 ccatccaaca tgatttctaa tgttctctgt ttcaaataaa tcattataag aagaaggaac
     3541 tgaaattgga taactctctt tttcaaccat tggttctaaa gcaagttctt cttgtaagtc
     3601 atcatttgta tctaacttaa atctccaaag accgcttaaa tcaattactt gcctcgtctc
     3661 tgtcataatg ggatataaca tgtaaatcac tcctatataa tttaaatatt aaataaatgt
     3721 atcctttaac ccacaataag catctattgg acttattccc ttaaattcag aaaggcccat
     3781 tagtttgtcc actaatattt ctaaagtttc ttgttttcca tcataagtat tgatataagt
     3841 ttttacttga ggcacatcag ctaaatgaaa cggacactgt acagatacaa aaattgttgg
     3901 aatttcatga atataaaatg gaatatccgg agtaccttta cttgctggcc aaacaattct
     3961 ttgaactgta cctgggttaa cattagctat attaataatc aagtcatatt tttcagttaa
     4021 atctttgatt ggtctcttag cagcatatat tccagctata tttttaattt tttcttcttg
     4081 cgataatcct ttcaattctt cttcaggtga aatgtatttt tcaacttcaa atccttttgc
     4141 tgacaaaatc tgtttcaata aatcaatggc ctgaggtcta tttccagcaa ctacttcacc
     4201 aaacccacct tttaccccat gtacatctac cagtaaaatc cttttatatt tttctggtac
     4261 aataggccaa atatcttgtt tatctttaac tagtgtaatt gcttgatcag ctacctcttt
     4321 aaaaattgat ttattttcaa ttaaaccaat tttttccata gcttcttgtt tcggtaacaa
     4381 aattttattc ttttcttttt tatgcaaacc tagtttagcc tttaagccta aaattcttct
     4441 taatgcatca cgtaaacgct cttctgaaat aattcctttg taataacctt ccatcatata
     4501 attaaagtct tcatctggat cattaaaaaa taggaataga tcacatccag ccgcaataga
     4561 agtgggcaat aattctgacc gtttcattgc cccagtcatt cctaacatat gactagcatc
     4621 agtaactact aatccattaa acccgagttt tcctctaagt aagtcagtga ttaattcttt
     4681 tgataaagtt gcaggaagta aatcttcatc tttcagatca agattaaaat gttttgaata
     4741 agctggcata tggatatgac ctgccataat tgaaggtaat ccagcttcta ttaagcctga
     4801 gtatactttt ccaaaagaat tatcccattc ttctgtactt aaactattaa tactaaaaga
     4861 tagatgttga tctcgttcgt caataccgtc accagggaaa tgtttagctg caggcaaaat
     4921 tccactctct tgaatacctt tcatataagc taaactaagt tctaaaactt tatcagggct
     4981 ttgtccaaaa gatcgactag aaatgatagg atttctccaa tttctattaa tatctacaat
     5041 aggagcaaaa ctccagttac atccaatagc cgatgcttca attccagaaa ctctccctaa
     5101 ttcataagca tatttcgcat catttgttgc accaattttt acttctacac caacttcggt
     5161 accatctgta caagctccgt taccacctgc ttctgtatta gcagcaatta ataaaggaat
     5221 tttgctttct gtttgtagga tatagttttg atcatatacc tcatcagcag ttcctggatt
     5281 atatcgtaca gctccaatat ggtactgatt gaccatacta cttaagtaat ttttatcacg
     5341 actagatccc atattaacaa ataattgacc tattttttct tctaaagtca tagcatcaat
     5401 cgtctcttca atccaagaaa tagcatcttc atctaaatga tatggttttt ttcgtaagtc
     5461 tacttttata gtcattttct ttctcctcta cttattagta agatttttcc aaaaagtatc
     5521 tatatatttt tgttcaatat ctatatactc tcccgataaa catttaaata aaatatcagt
     5581 tcttaagttt tcccatgatt ctttttcttt attaaccaaa ataggataaa agaaaacttt
     5641 attcttattt gcagcttcta gatcaccagg agaatcacca atcattaata tcttatccaa
     5701 atcacaacca ttttcaatta cacttcgaat aatgtcttct ttttttcctt tatcttgaca
     5761 acacagttca tcaactaagg ataataattc atgtctttcc cattcttctt gaatagcttc
     5821 cttattagct gagcttacaa taattatctt cccatatttt ttcagagctt ttatagtatc
     5881 tctagcactt aaaaagggtt tatctaatcc tttagcttga ctaatctgtc gattgacttc
     5941 ttccgaccat tttaaagctt ttaataaatc ctctgactga gagtattcta tttccctctt
     6001 caaagatgga ttagacaatt cttttgttac agatacccag tgctttaatc gattaatcga
     6061 tggtacatca tttttttcta atgttttaac taaacctaaa aatctgttta ttccacgagt
     6121 ctctgaatat aaattaatat tattccaatc ctgtaggaat ttttccctat gatgaacatc
     6181 aaaaatctct gcagcaattg gcccgaaaaa gagattgtgt ttataagtca ttgtatccat
     6241 gacacaacca tctgaatcaa tacaaaatat ccaactgttc atgttttttc tcctagttat
     6301 gtttttgatt tagcaaatta taattttcta atactttttc tttagataat ggatatacga
     6361 aaaataaaat caatcctaca acaaagaaac aaactgctgg aatcaacgtt gaagagttat
     6421 acagcttttg taaaacttct ccactttgta cttttgctaa cgaatcatac cctattatag
     6481 aaagagtcca acctccgaca ccgccagcta aagcttgtcc aatttttcgg gcaaaagaat
     6541 atactgcata aatagttcca tcttctcttt ggccagattt cacttcttga tcatcgatta
     6601 catcagtaat attcgcccaa atgactgtat taaaaatacc taaccccata tatccaacag
     6661 aagtaagaac taagaataca tacatattac taggctttat caaaaataaa agtaaatata
     6721 caatagcaga gaagaacatc cctactgacg ccaattcttt tttaacgtat tttttactga
     6781 aaattaaact tacaggtgaa acaagtacca acatcaaaat aggattgata aagttcatta
     6841 aaactattcc ctgtgcatta ttataataat ctggaaatac ataattgttc atactcattg
     6901 ttaaaagctg agcaagcaac aaaaaaatag ctgcagctat tatagaaagc aacgctctat
     6961 ttttagctaa tccttttaaa gatttcaaca atgaaacttt taactgcgat tcatcacttt
     7021 taacccgttc tacagttaat ttataacata ttacgtaaca aataactgca cacactgaaa
     7081 atactcctgc cacaattgta aaagttgatc caccacgtac aatttgagct ccagaactat
     7141 ctcttgtata aataaaaatt ggtgttaaca cgccaatgaa catacctgct aaggtagctc
     7201 ctatactacg aaatgttgat aacgctgtcc tttcttctgc aacagaagtg atagctgatg
     7261 ccattgaacc gtaaggaata ttaatagatg tataaaaaat acttccccat agtatatatg
     7321 ttacatacat atatataact ttaatactca tagaagctga tgccattgat gtttgataca
     7381 ttaaaaaact tgctaaagcg actggaccac tcatccgtaa aatccacggt ttaaatttgc
     7441 cagttttaga tggttttgat ccatccacaa tacgccccat tgttatgtct gtaactgcat
     7501 caataaatct tgctactaaa aataatgttc ctacggtagc tccgcttatc cccaatactt
     7561 ttgtataaaa aatcattaaa aaagaactag caaaaatgaa agtgaaatca ttcccaaaat
     7621 ctccaaacat atatcctatt ttgtctctta gaccaaattt tctttccata taatttgcgc
     7681 tccttcctat tgttaagcgt tttcattctg ttgaactgcc aacttacgta ctatactctg
     7741 aagtataaag agttagtatt tattttttac attttatagt attatttatt ttttctccta
     7801 ataattcttt atctcataaa acttatctat atatttacta gctattcgag ttacttcttc
     7861 aaaatcacct ttttcaacag gagccaacaa attcccacct acaccaactg caacaacgcc
     7921 tgcttcaaac cactcttcta agttatctag acttactccg ccagtaggca ttatatttac
     7981 atatggtaat ggagcttgat acgtttttac aacagatgga ccaaaagcag aacctggaaa
     8041 tagtttaatg atttctactc cagcggtaag tgcctccttt atctcagtaa ttgtcataca
     8101 acctggtaaa taaggaattt gatataaatt acaaatctct gctgtttgtc gatcaaaaga
     8161 tggacttacg atatattctg ctccagccat aattgcaata cgagcagtaa acgcatctaa
     8221 aactgtaccg gctccaatta ttacatcttt ttgatcagca tattttttag caatttcttt
     8281 gattatttct tcagcattcg gaactgtaaa agttagttca atccctttaa ttccacccgc
     8341 agtaattgca tttattgctt taatggcctc atctttattt tttccacgta caaccgcaat
     8401 gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta tctccttctt
     8461 ttatggatac atactctagc tagttgtact aacttacaat aaataaattt aagcttttat
     8521 tttttgtatt attttctact ttcgatttca aaaaaattaa aataattata cgcattacga
     8581 taacaaatat tctccactat ctgactaaga tacatttcat catctggaat tcgacctgct
     8641 ttaacccact cgcttagtaa ttgacataat actcgacgga aatactcatg gcgaggatag
     8701 cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa actttgttgc
     8761 gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca ccaagcacat
     8821 cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt tgctagtgga
     8881 agccaatcgg ttggatttaa tgaatataaa attgtttttg gtaagcaccc actcttttca
     8941 gcagtgtcga acaatgctac aacattttgt gtcaaactag cttgatcccc catcgaatca
     9001 ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct taaagcattt
     9061 aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg taattgaata
     9121 cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc taaagctttt
     9181 attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt attcaaaccg
     9241 tgatctgcta atcgacagcc aatttgatga aaataatcca ctcgttggct caatgcctgc
     9301 ttcaacccat taaagttttt gattggtata cccgctgctt gactaagttt taaaatatag
     9361 ttcgaaaatc ctgatgattt gatactccag accttatctg gacggaaagt aggtaatacc
     9421 ttgaaagaat tatcctgttt agaaagctca gtatgatatc gcaagtcatc tgtaggatca
     9481 tccgttgtac aaatggcttt cacatccatc atcttcagca attcttttgg gcgataggaa
     9541 tccgtttgga ttttttcgtt ggcacgtcgc caaatttctg gtgcattctt ctcagaaatt
     9601 gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa aataggatta
     9661 cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc attacctgca
     9721 ccactgatca actcttcttc tatcccatta gctcgcatca atcgccattt ataatgatct
     9781 ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt attttctagc
     9841 aaacatgctg ggtctaaatg acaatgataa tcgatgattg gttggtgttc tgcaaattta
     9901 tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa aaacatattt
     9961 aagcactcct ttccttccct tagtttttag aagagaaaat ttttcttcta aatagactcc
    10021 attatataat tatttactcg cccccttttc aagcgcttcc caaataccat ttaaataaac
    10081 tgcacctaga gctcggtcat ataacccata accaggtcta ccggtttctc cccaaatcat
    10141 ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg tttttaatat
    10201 cttatacata tctagagaac catattctga tttatgcggc gcttcataaa aatcgagtcc
    10261 attatcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat cccgttgaca
    10321 aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac ttccagtaca
    10381 taacgtaata ccattagaag gagaatccac tacattacac aaccattcta aatcgtcacg
    10441 atttttaaca atacgaggta atccgaaaat agaatatggt ggatcatctg gatgaactgc
    10501 cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat atgcaaagtt
    10561 ctctcgcaat ttcgcttcat caacttcttt ataggcttct aataattgct tgatttgtga
    10621 taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt ctacttttct
    10681 aataatttcc tctggatcag taggaatatt ttttgaaata taggctaacg tagaagaccc
    10741 atcatctaaa tgataatcca aatctgattt tacccaatca aagactggca taaaattata
    10801 acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat aattctcgat
    10861 gtattgatct cgtgttggca aaccaatttt gatatcatca tgaacattaa cactctcaat
    10921 aactttcaag gtaaggccat tggcttcgac ttcatctttt aaagctttaa ttcgttctct
    10981 cggccagacc tctccggctg gtatatccat taatgttcca acaattcctt tcattgctgg
    11041 gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc atctaaaagt
    11101 catttccata gttactttcc tccaacttct gttatatatt tattgattgt tttacgaact
    11161 gcttgaggac ctactagcat ttcgcaaaag attttttcaa ttttctttcc tatctcaact
    11221 tgatataaat ctaaaccaaa tatttcctta ttttctaaga tttcctgtac cgcacaatga
    11281 atctttgcag gatcttgttc acctaatttc aatactgaga atttttcctg caactctacc
    11341 aataaaggat caggacttgg cacaaaattt tttccttgat catcgactgc caataaataa
    11401 cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt tccgtctttt
    11461 tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga agtatctgtt
    11521 gcaattcgtt gcggtgtatc tggaatatag ggattaggca aacgtttatt gattacttca
    11581 tcaataaatt gttttggatc aataacgcct ggactttcaa ctactggtaa gccttctaca
    11641 taacctatcc ctttaattaa gccaaccaaa tcatcatctt gcatttctgc ctgaattgat
    11701 ttatacccca acaaacaacc tgttattgct aatgccgtat ggagcggatt taaacatgtc
    11761 gtaaccttca tgatatctgc tttatcaact gtttctctat ctgtcataat cacaccagcc
    11821 gcttccagtt taggtcgacc atttggaaaa ctatcttcaa tgactagata atggactttt
    11881 tcagtattgg taaaagcagc gatattggta tttttcgcag tatgaattat atctaagtct
    11941 tcgaaacccg ccttcaataa atccattttg acactttccg aaggatttgg cgtaattcta
    12001 tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc aataaatttt
    12061 tcagaaacgt atcctttttt ttgccaagct tctgcaatca taaaaatgct atcttgaaaa
    12121 cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggtaa ctctccagca
    12181 ttaaacctgc tatacaacaa ggatgtcaca atactcatcg tatgttgagg attggtaggt
    12241 ccagatttta tatctgcttc cactaccgaa aaaaattgtc ctttagaatc ttttaaactg
    12301 tatccttttt cagtaatagt aaacgttgta aactgtaatg taggatcttc aaaatattta
    12361 accactcttt gatagtccgt tatattcttt ggattgcaat ataaactatc agctgttact
    12421 gctaaaagtc tttttttgag ttcaccatct tcacccataa caatctccaa aatatcattg
    12481 ttgtagtcat gataagcttt ttctactact tcttcatcaa atgtctcaca cacaactact
    12541 ccggattcta attcattttg atctgctaag cattgagcaa tttctgcatg aaatcctcga
    12601 taaagatttc ctccaccaaa atgaagccac actggatgtt tctgaccttc ttgtttgacc
    12661 gcttcgacat taaaatacgg tagttgaact tgcattttgt tcaatccttc gatagaatct
    12721 tgccagtctt taatcaaaac catatttacc cctccatact gatatgttag tttccgttac
    12781 aattgtagta taaaatcgaa tgtaatcgct gtcaataaga aaaaactaaa aatattcttt
    12841 tttctaccat gtcagaatta ttgtgtcttt ttttagtaaa agcacaccaa tatatatttt
    12901 ttagaactaa cttaaattaa agcttaattt ctctttgatc ttatttgagt attttttgtt
    12961 tttttaatag ctagtatgtt actttgaaat ttgatattat ataacgagca tagaactaaa
    13021 atatcaaatt caaaaatgaa attttgagag ggattaaata tgacaattaa aactagtctc
    13081 caaaatcaag cttatcaatc tattcgtcag caaattatct acgctgactt agaacctgga
    13141 aaaaaagtat cagaaaaaaa attggaagaa acgctttcta tcggtcgtac tccaatacgt
    13201 gaagcattga ttcaactccg tcatcaagga cttgttgata caattcctca aagtggaaat
    13261 tatatctcat taatcgactt agatttagca aaaaatgctc gttttgtcag agaacattta
    13321 gaacgacaaa ttatgctaga gtgttgtgcc aagatgaata atcaaaagaa acaagtttta
    13381 gaaactataa ttgcagaaca agaaaaagct gttagtcaaa agaatgctcg tagttttttt
    13441 cataatgata atttatttca taaagcttgt tttgaaattg cagaacggga agaagtatgg
    13501 caatggttgg aagataataa tacgcacttg gaacgatttc gttggttacg agtaaaaacg
    13561 gaaggattaa aatgggaaaa aattatggaa caacatcatc aactatttaa agccttgacc
    13621 atgcaagacc ctgaagaagc taacttctta acatccttgc atcttcattt aatgcttgat
    13681 gaacaaaatg ttgtattgac tactcaccca aaatatttta aaaatatata actgctaaat
    13741 ttcttttttt taatttattt acatttctga ttactacaga aaactaacta ctttttttat
    13801 gtaaagttct gtcaaatata gtcactttat attactttct cttgagtaaa aaaacatgtg
    13861 aggtcgtaaa caaagattga actatctaca ttcatactct ataatctgtt tatttgcaga
    13921 ctgataatag caaaagaaaa cttataatca aataatatca acatttgatc acataatttg
    13981 agattgaatg ctgatttacc tatgttttac gtaatggtct ttagttattt ttgctcataa
    14041 aatgcataaa acacgcgttt ttatgtgtgc ttgaaatttc ttggctctat aatttctaag
    14101 actgataaaa atattctatc ggttcttttt gcaaataaaa aaacatatag aagctccatt
    14161 agattgaatc accacaacac aatcaaagga gcattccgta tgcttgattt tcattctata
    14221 aaactacttt taggaatcaa agaaaatcac aacggttcaa actaaaagca agaatatccg
    14281 aagtaccatt gtttcatcga tcatgagaaa agcattcacc tctgtctgca gatgttctta
    14341 tatcgctctg tcagtcctat taataaagta ctgttttgtt ttcaaaaaaa ctattacgag
    14401 tactttttaa gagaatgcct tgcaaatatt catcacacac cgttatataa gtcaaatagt
    14461 ttctttcagt cgtcaagaaa agattctcct gtccttggca ttatcagtta agtagcaact
    14521 taattctcgg acttgttcta ctctagcaaa atttattttc atcaacttac gtccaaatgt
    14581 ttttttgaat aattacttca tcgcgggtct ggaagacttg cctcattcga taagtcacta
    14641 gcttttggcg tccttcagga tttctagtat acaccaactg acatttgggc agcgacgatt
    14701 gatttaattc gtttagaaaa aggttagcga tttgcttatt tcggccaaag cgttctggta
    14761 ccgtcagaac tatctttttc ccttcgatta aataccgcgg attctccagc ggtgctaaaa
    14821 gctccctcat agcttcagta aaaagctgtc cttctcgata gccagcattc tcaagatagc
    14881 atctaaattc ctgctgatca gccgctacga ttacttttct atcactaact tcaccaaggc
    14941 gctgcaacgt cgtttgaacg catatggcca agcttttttt gcgggcacat tccttttccc
    15001 atagcttttg tcgcctacga atttggaaat attccagcaa gttaccttta aatatagctg
    15061 accagaagct tagaggctct atatttttta ctactatcgg caatgaagtt tcttgttttt
    15121 gctttacctg atcgactttc ctaacgacga ttggcataga taggtgccag ccatcagcta
    15181 aagcagtact ccattgttct ttcaaccgac ttcgttcaca agaaaaatag aacatcgact
    15241 gagtataagt ttccatcgtg gtggtcgtat attccagtgg taaagcaaag cgatcgccac
    15301 cactctcaat ttttggtgaa gattcaaagg tcagacctga aatattgccc aaacgccgta
    15361 gcattctttt caaatcaggc tgttcagaca gaggaactcc cggtaatcca atcgctaaat
    15421 gccaaatgtt cgttacttta ttttgtgcac ctaggcgtaa gccccgccct cgtagttgtt
    15481 gtgtttgcac aaaagtggaa gcttgattag ccaagatcaa cagattaatt gcaggacaat
    15541 cccagccttc tcctagcaaa ctaacggttc ctaccaaaat attcaacttc ttttcggtta
    15601 ataggcgagt aactgcttgc atcaatgctt ggcggttttt gtcatccacc gtcaaataaa
    15661 aataacccga catataagga tgttcattca tacccgtcgc tagttctgcc agctcaacag
    15721 ttactagcat tttcggtaac agtaagaact ctccacagat ggcaccgatt tgctgagtgt
    15781 tacctaatag acgaacaact tcatcaaaca aaggaaccac gccataagct aaggtaccat
    15841 cactgcctgc taatacctcc ttgcaaatac gatccatcaa aatgaccgct gtcagttctt
    15901 gcttcagctg aacttcttct tttaacaaaa tttgacaaac tgcttgcttt ttctcaggaa
    15961 tcgcctgact accaacagtg tctggatact ctggaaacaa atgcagcgac tcatcaaaca
    16021 atcatccagc ttttttcaat tgataaaaaa taggcaattc cggttgatct tcaaacatct
    16081 gctgataaat agtgatcagc tcttttttac ctaaagatgg aactgaagct tttttacctt
    16141 gctgcttaga actgcctaat agctgccaat gttcgggtat caattcatag ttatgatgat
    16201 tcaagtaaat taatgcacta gtgaacagct caaaattttc ataaatttcc ttcgaatgct
    16261 caagcggatg ctgaacaaat tctaaagaaa gcaaataatc caccgtctct tggctttcca
    16321 ttaacgcttc caccagctca ttttgtttct ctaaaaaggt aaaataatgc tgagtagttt
    16381 cctgattagc cggaatcaaa tatagaaaat cctgatgagg acatagaacc tcctccttta
    16441 ccaactcggg aatgctaatt tcttcgtcaa tcaggccatt ttaactcaat atattgcttc
    16501 cactccgtct gagagctatc aatcggaggc gtggctgtca aggaaatcgt caacagattc
    16561 ggttgttttt tcttcaagtc aatcaacaat tcaccccagc ttttttttaa atgatgagct
    16621 tcatcaaaaa actaacagct ccagctgatc accataagtt ggctcctcct tcagtcggtt
    16681 gtaaatggtc tgatacgttt ccactgtaat atgactagga tgttcaatcg ccccagaaag
    16741 atagcaacta tctgctggat ttactaagaa atattcctcg attgttgtta accactgttt
    16801 cttcaatatt agggaaggaa ccaaaattaa ggttttacaa tccaaacgtc ggatcatctc
    16861 aattcctaaa aaggtcttgc cagcccctgg tggagccact agatggactg aattgttatt
    16921 taaataagta gcaaagttag tcaatatacg cttctgatag ctacgccact gaattttttc
    16981 atcagatagc cacatcccag gtccccctct cacgtatttt ctctatcata atctaaatga
    17041 atacagcaaa aaaaagattt cttcaagaat atgtgttttt atagtaatat tttacttact
    17101 ctaaactcaa atgaaaaggt aaaaaaactg gtagtacaat cttttaaatt ctcattgttt
    17161 tgtacgttct ttactatcaa aaaaacaaaa aaaacgcgtt tctacgcgtg accaaaattt
    17221 ttagctctat aatttctaag actgatagaa aatcattctg tttttgaagt cttttttcat
    17281 aaatactact tgatttattg tgtatattgt ataaaagatt gttcataatt attttttata
    17341 attaaaattt ggtattgatg accagcgatt ccgtatataa tgcgcaatca aattaagctt
    17401 tctgtctctg gtaaatattc ctgttctgtt ttcttgtact cgtaagattt cttcacttat
    17461 cgcaaaattc ataaaattcc acactttttc ataaacaaaa tgtacacatt tatctattac
    17521 ttcataatag tctacttgat attcttctgt aaacatggtt gctactgtat ctgcaccata
    17581 tttgataaac atttgggcta ctcccttaaa ttgagaaaag cccattagtt tatctactaa
    17641 tacttctaaa atttcttgct ttcttcttta acatcgtccc gtaaggaatc atgtagtatg
    17701 ctggaggaag ttcattttct aaataattgt atcgcttcaa ttgctcaaag gctattctct
    17761 acaattaatt acaccaatta ctttatcttc acttatataa gatttctaaa catttttcgt
    17821 caaatgaaga gaaaatattt ttttaaatga tcttgctcaa gatttgtcaa cgaatctttg
    17881 aattcgtttt gaagttctga ggaaggtatc atttaaaaac tcaatctgac tttcatcatt
    17941 tcgtttactc ccat
//
LOCUS       DACDCV010000057        17917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDCV010000057 DACDCV010000000
VERSION     DACDCV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.057"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IV041_001834"
     CDS             202..1077
                     /locus_tag="IV041_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382603.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IV041_001835"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IV041_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IV041_001836"
     CDS             complement(1642..2973)
                     /locus_tag="IV041_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382604.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IV041_001837"
     CDS             complement(3122..3835)
                     /locus_tag="IV041_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8382605.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IV041_001838"
     CDS             4010..5434
                     /locus_tag="IV041_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8382606.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IV041_001839"
     CDS             5733..6440
                     /locus_tag="IV041_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8382607.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IV041_001840"
     CDS             6427..6750
                     /locus_tag="IV041_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8382608.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IV041_001841"
     CDS             complement(6839..7876)
                     /locus_tag="IV041_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8382609.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IV041_001842"
     CDS             complement(8197..9180)
                     /locus_tag="IV041_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8382610.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IV041_001843"
     CDS             complement(9595..10365)
                     /locus_tag="IV041_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382611.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IV041_001844"
     CDS             complement(10358..11293)
                     /locus_tag="IV041_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382612.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IV041_001845"
     CDS             complement(11609..13258)
                     /locus_tag="IV041_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8382613.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IV041_001846"
     CDS             complement(13375..15978)
                     /locus_tag="IV041_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8382614.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IV041_001847"
     CDS             complement(15990..17039)
                     /locus_tag="IV041_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8382615.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IV041_001848"
     CDS             complement(17065..17379)
                     /locus_tag="IV041_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8382616.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IV041_001849"
     CDS             complement(17395..17841)
                     /locus_tag="IV041_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8382617.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         5492 a         3627 c         2962 g         5836 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatga
//
LOCUS       DACDCV010000058        17889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDCV010000058 DACDCV010000000
VERSION     DACDCV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.058"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IV041_001850"
     CDS             complement(45..1511)
                     /locus_tag="IV041_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8382618.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IV041_001851"
     CDS             1898..2494
                     /locus_tag="IV041_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8382619.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IV041_001852"
     CDS             complement(2552..3034)
                     /locus_tag="IV041_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382620.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IV041_001853"
     CDS             complement(3140..3337)
                     /locus_tag="IV041_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382621.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IV041_001854"
     CDS             3632..4390
                     /locus_tag="IV041_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8382622.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IV041_001855"
     CDS             4450..4878
                     /locus_tag="IV041_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382623.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IV041_001856"
     tRNA            4924..4996
                     /locus_tag="IV041_001856"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IV041_001857"
     CDS             complement(5146..5517)
                     /locus_tag="IV041_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8382624.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IV041_001858"
     CDS             5732..5917
                     /locus_tag="IV041_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382625.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IV041_001859"
     CDS             complement(6000..6188)
                     /locus_tag="IV041_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382626.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IV041_001860"
     CDS             complement(6212..6475)
                     /locus_tag="IV041_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382627.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IV041_001861"
     CDS             6633..6890
                     /locus_tag="IV041_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8382628.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IV041_001862"
     CDS             complement(6961..7152)
                     /locus_tag="IV041_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382629.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IV041_001863"
     CDS             complement(7293..7613)
                     /locus_tag="IV041_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382630.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IV041_001864"
     CDS             complement(7636..8070)
                     /locus_tag="IV041_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8382631.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..9043
                     /locus_tag="IV041_001865"
     CDS             8405..9043
                     /locus_tag="IV041_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8382632.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9367..9450)
                     /locus_tag="IV041_001866"
     tRNA            complement(9367..9450)
                     /locus_tag="IV041_001866"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9414..9416),aa:Leu,seq:cag)
     gene            9978..10478
                     /locus_tag="IV041_001867"
     CDS             9978..10478
                     /locus_tag="IV041_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382633.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10668..11012)
                     /locus_tag="IV041_001868"
     CDS             complement(10668..11012)
                     /locus_tag="IV041_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8382634.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11166..12086)
                     /locus_tag="IV041_001869"
     CDS             complement(11166..12086)
                     /locus_tag="IV041_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8382635.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12273..12764
                     /locus_tag="IV041_001870"
     CDS             12273..12764
                     /locus_tag="IV041_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8382636.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12788..15163)
                     /locus_tag="IV041_001871"
     CDS             complement(12788..15163)
                     /locus_tag="IV041_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8382637.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15489..16115
                     /locus_tag="IV041_001872"
     CDS             15489..16115
                     /locus_tag="IV041_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8382638.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16138..16842
                     /locus_tag="IV041_001873"
     CDS             16138..16842
                     /locus_tag="IV041_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8382639.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17034..17867
                     /locus_tag="IV041_001874"
     CDS             17034..17867
                     /locus_tag="IV041_001874"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8382640.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5553 a         3489 c         3015 g         5832 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     8821 aagtacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     8881 aacgaacgga cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta
     8941 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     9001 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg
     9061 tttaaacaca ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt
     9121 tttgagttta agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag
     9181 aatctataaa tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac
     9241 aaattttttt tgatttattt gaatctggtt ccgttgttta aaatttttcc tatattcttt
     9301 tagataatat gttattttct ttctcatcgc tccaggtgta ccttctcttt ataaaaaaac
     9361 aaattatgcg gctaagaaga ctcgaacttc cacgaggtca ccctcactag cgcctgaagc
     9421 tagcgcgtct gccaattccg ccatagccgc atcacatctt ttagtttagg attacatgtg
     9481 ttttttgtca agaacatatt tttgttcttt ttcatttctg tacgccctcc cttatgccat
     9541 ctacacaaat aattcatccg agcaactcca tcatctattg agggttggtg aaaaaatctg
     9601 ttttatccat atgatcgatt tcaatgttaa acgaacgact ctgggattag taactatttt
     9661 atttttttct tcatttcttt tttcctcttt ttctcgattc agctatcttc tttcatttgg
     9721 atattgatta tttcttctgt tgattactat ccttctggaa ttctagacgc tgaagtaatt
     9781 cacgttttct ttcttctaga gatcataagt aacgaatgaa cgaacttcaa ataatttttt
     9841 tattgtaatt catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa
     9901 aaataggcat ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac
     9961 ttaaagaggt gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt
    10021 atcagctaca aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct
    10081 atacatttgc tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg
    10141 atcttcctga gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt
    10201 atgtaggaat ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc
    10261 atattggtta cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag
    10321 cattagtact acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata
    10381 aaaaaaatct tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag
    10441 atgagaaaga atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa
    10501 agggatttga atgtccttta tcaatagaac acatttattt aattaactta taatatagca
    10561 gacaaaattt tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc
    10621 ttttttgttt ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc
    10681 aacgcaatcc gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt
    10741 ttgacttctg ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt
    10801 aaaatatctg ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca
    10861 ttttcaggat tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat
    10921 tcaggatcaa tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct
    10981 tgtccagacc gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta
    11041 tttatcatac tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt
    11101 aaatttataa aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg
    11161 attcctcatt ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac
    11221 attttttgtt cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg
    11281 ataaatgcat cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg
    11341 gtgatgacca tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt
    11401 aatgtcattc cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg
    11461 gtaacgatca ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc
    11521 attgcataaa tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg
    11581 atgactacaa tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt
    11641 tgtgaaagta caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat
    11701 aatcctacgc caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca
    11761 gtgatctgtc cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa
    11821 ctaaggatcg ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc
    11881 aaaagaaaaa taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag
    11941 gaaatgattt gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa
    12001 taatttaaag cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat
    12061 ctttcatttt tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg
    12121 tattccaaat gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga
    12181 gaagaatttt agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg
    12241 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
    12301 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
    12361 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
    12421 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
    12481 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
    12541 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
    12601 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
    12661 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
    12721 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
    12781 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
    12841 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
    12901 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
    12961 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
    13021 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
    13081 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
    13141 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
    13201 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
    13261 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
    13321 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
    13381 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
    13441 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
    13501 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
    13561 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
    13621 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
    13681 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
    13741 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
    13801 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
    13861 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
    13921 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
    13981 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
    14041 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
    14101 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
    14161 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
    14221 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
    14281 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
    14341 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
    14401 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
    14461 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
    14521 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
    14581 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
    14641 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
    14701 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
    14761 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
    14821 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
    14881 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
    14941 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
    15001 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
    15061 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
    15121 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
    15181 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
    15241 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
    15301 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
    15361 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
    15421 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
    15481 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
    15541 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
    15601 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
    15661 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
    15721 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
    15781 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
    15841 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
    15901 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
    15961 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
    16021 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
    16081 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
    16141 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
    16201 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
    16261 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
    16321 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
    16381 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
    16441 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
    16501 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
    16561 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
    16621 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
    16681 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
    16741 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
    16801 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
    16861 taccagagaa tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg
    16921 tgtacttatc tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct
    16981 caaaaaataa cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt
    17041 ttgcaaatat tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc
    17101 ccatcagtag tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt
    17161 cacaatttat gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg
    17221 ttctttattt tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag
    17281 atacgtggac actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt
    17341 tgcctttgga tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt
    17401 tgatcgttta cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc
    17461 gttggacttc tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag
    17521 tcttggcact tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga
    17581 ttggtgcttc gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt
    17641 ttggcgcaag cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg
    17701 aagaaactgt tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta
    17761 tcaaattctt actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta
    17821 ttatccttgg tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac
    17881 acttctctt
//
LOCUS       DACDCV010000059        17509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDCV010000059 DACDCV010000000
VERSION     DACDCV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.059"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1028
                     /locus_tag="IV041_001875"
     CDS             <1..1028
                     /locus_tag="IV041_001875"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382641.1"
                     /translation="LTVFLPQITEYLNYRIFTTKVDDSVTSLSRLEFIKICASVFGPI
                     LTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREFYFLLNMFIEIQ
                     KDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSGPLKGTVEYIKF
                     ETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFKMFHRIVKTLNE
                     YYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDD
                     FDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYK
                     LFNETKL"
     gene            1072..2244
                     /locus_tag="IV041_001876"
     CDS             1072..2244
                     /locus_tag="IV041_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8382642.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            2231..2935
                     /locus_tag="IV041_001877"
     CDS             2231..2935
                     /locus_tag="IV041_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8382643.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2940..4100
                     /locus_tag="IV041_001878"
     CDS             2940..4100
                     /locus_tag="IV041_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8382644.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            4176..4454
                     /locus_tag="IV041_001879"
     CDS             4176..4454
                     /locus_tag="IV041_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8382645.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4435..4806
                     /locus_tag="IV041_001880"
     CDS             4435..4806
                     /locus_tag="IV041_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8382646.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4822..5226
                     /locus_tag="IV041_001881"
     CDS             4822..5226
                     /locus_tag="IV041_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382647.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            5223..5633
                     /locus_tag="IV041_001882"
     CDS             5223..5633
                     /locus_tag="IV041_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382648.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5694..6281
                     /locus_tag="IV041_001883"
     CDS             5694..6281
                     /locus_tag="IV041_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8382649.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            6354..6821
                     /locus_tag="IV041_001884"
     CDS             6354..6821
                     /locus_tag="IV041_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8382650.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6867..7175
                     /locus_tag="IV041_001885"
     CDS             6867..7175
                     /locus_tag="IV041_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382651.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            7370..14104
                     /locus_tag="IV041_001886"
     CDS             7370..14104
                     /locus_tag="IV041_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8382652.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            14108..14980
                     /locus_tag="IV041_001887"
     CDS             14108..14980
                     /locus_tag="IV041_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382653.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14977..15604
                     /locus_tag="IV041_001888"
                     /pseudo
     CDS             14977..15604
                     /locus_tag="IV041_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15565..16026
                     /locus_tag="IV041_001889"
     CDS             15565..16026
                     /locus_tag="IV041_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382654.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            16030..16854
                     /locus_tag="IV041_001890"
     CDS             16030..16854
                     /locus_tag="IV041_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382655.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16872..17417
                     /locus_tag="IV041_001891"
                     /pseudo
     CDS             16872..17417
                     /locus_tag="IV041_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         6289 a         2660 c         3706 g         4854 t
ORIGIN      
        1 ttttgacagt atttttacca caaattactg agtatttaaa ttatcggata tttaccacaa
       61 aagtagatga tagcgttact agtttaagta ggctagaatt tataaaaata tgtgcatctg
      121 tgtttggtcc tattttgact gtaatggtat ttgtgaatac tttaaatatg cagaaaaaaa
      181 cagagcaaaa gcaaaatgag cttgaagtaa agcaagctaa ggctgagaac gagagagcaa
      241 aaaaagagct gttaacacag atagatcgtg agttctattt tttattaaat atgtttattg
      301 aaatacaaaa agatcaaaat gccaaacaag ctgttattgc tttacagaag ttggcaattt
      361 ttaacgaaaa gggttttaat caagactatg catttacttc taaagattta tcagatagtg
      421 aaattgggag tacaattgaa caatcaggac cgctgaaggg cacagttgaa tatataaaat
      481 ttgaaacatt tgatgtaagc aagtcgatgg ggaattatca aacaaaaaca aaagacgttg
      541 ctacaagttt acacgatcag aaagtagcta tattgaatga acaatatgaa aagatgcaaa
      601 tatatcttgg acggtacttc aaaatgtttc accgaatagt taaaacatta aatgagtatt
      661 atgacgatta taatgatttt gatgtgaaga ggtatacaaa atatataggg acattaagaa
      721 cacaaatttc tccagctgaa ttccaagtga ttttattcaa ttctctatat ataaaacgag
      781 gttttggctt aggaattcaa cttattggaa gtggtttttt tggagatgat tttgattttg
      841 aaactaatca acattttgaa acaagtatca atgaacagtg gtttctatca ctatcaactg
      901 ttgacagtga taactctata aagcgacaaa aattatctga atatattaaa gaattaggat
      961 ccgtagaata taaaaaaata gctaattttg aaagtttata taaactcttt aatgaaacta
     1021 agctctagaa atagggcttt tttatttttc tttgaaaggt ggtgaaaaca tgtgagctta
     1081 tttgatatct ttaagcagtc aataagaaat gaagagcctt ctgagtggat accggatttt
     1141 gtatatggag aggaagtatc tgctcgggca tacctcaaaa ttatggcaaa aaatactgtt
     1201 ttagattttg ttgcaagaac tatgtcaacg ttagaagtta agtttaaaaa taaagatggg
     1261 acatcagact gggattatat tctaaatgtt cgacctaatt ctgatatgtc agcagctaca
     1321 ttttggcaaa aattttttta tcggctctta gatgacaacg aagtgttagt aatatttaca
     1381 gatgataatc aattgctgat tgctgatgat tttagtcgaa cggagtatgc cgtgtatgat
     1441 gatgttttta caaatgtgat ggtaaaaaat tttgtctttg aacgttcatg gaacatgtct
     1501 gacgtcattt atatggaata taataacgaa gaattagaaa ggtttacacg aggtttattt
     1561 gaagattacg ccgcgttgtt cggacggatt ctagaagttg caatgcgtaa caaccaaatt
     1621 cgtgggtctg tatccattga ttcaacggga acggcgaatg atgaaaaagg aaaagatggt
     1681 aagactagat ctcaaagatt acaagaatat attgatagga tttggaactc ttttaaaact
     1741 aactcagttg caattgttcc caaagtgaaa ggctttgagt atgaggaata caccaataaa
     1801 caaggcgtat cgaatcaatc gctagatgaa ctgaataaga tgaagtcgtc tttaattgat
     1861 gatgttgcca acgccatagg agtacctacg gcgcttattt atggtgaaaa agctgaactg
     1921 gattcaaata ttaaagcttt tcgtaaattg tgtattgctc cactagttaa aaaacttcaa
     1981 gacgaattgg tggcaaaaat aattagtcgt caagagtaca aaaacgggga acgtatagaa
     2041 gttaaaaaag ttctccaaaa agatctatta gagtatgcta ctcaaattga taaaataaca
     2101 tctggtggtg cgttttatat tgacgaagtt cgtgatgaag ttgattatga tgagttgcca
     2161 aataatgaag gaaaaattcg tattcgtacg aaaaactatg aagaaactgt gaagggaggt
     2221 gagaacgaca atgccgaaag ctaaaaaagt accatttcag tttactaacg aaattcagaa
     2281 tggtaagcac attctcacct tgagtggaaa tgtacagaaa aaatattggc gtgatgatga
     2341 tgtcataaac gctaaagata ttcgtgactc tctagatact gtaaccgatg atattgtaat
     2401 caagttgaac agcccaggcg gtgatgtgtt tgaagggatt gagatttaca attatttaaa
     2461 agatcaccca tcaaatatta ctgtagaagt tacagggctt gccgcaagcg cagcaacgtt
     2521 tattgtcgct ggtgccaatg atgtgattat gaatgttggg acttctttga tgattcatga
     2581 agcttctaca tttgcttggg ggaataaaca ggacatccaa aaaacgttaa acgcattgga
     2641 aacgatcgat gattctatct tagctattta ttcagataag actggccaat ctgttgataa
     2701 gttgcgtgaa tggatgaatg aagaaaaatg gtttacagca gatgaagctg tcaaatatgg
     2761 atttgccaat tctgtgaagc gtgaagaacc acaaaatgaa cctcaaaata ttgcagcgat
     2821 gattcaagat gctgttgccg ttgcaatagc gaatttaaag gaacctgtta ctaataaaat
     2881 cgaacaagaa tcaaaacaaa aatcattaat cgcacgattg cgaaaaggag aataaagata
     2941 tgttaaaaat tacagataaa actgcagatg caaaaaaggc atttaatgct atttcttcga
     3001 aagaagatgc aacacccgaa caaattaatg ctgctctaga agaatatgta accgccattg
     3061 cagaagatgc cggcaaacag gtgcgtgctg aatatgaaga attgaaaaat gtgacggaca
     3121 accgtgtatt agaggctcgt ggaattccta cactaacagc agaagaaaca aagttttata
     3181 atgaagccgt aaaatctgga ggtttcgatt ctgacgtagt ttggccagaa acgattttgg
     3241 aacgtgtttt tgaaaatctt caaaacgatc atcctatttt aggcctcatt aactttacac
     3301 caactgtagg tcgtgtcaaa gtaattagag cacgtcgtaa aggtgttgct gtttttggac
     3361 cattgcataa agaccttgaa ggccaactag atgctgaatt tggtgcaact gaatttgttc
     3421 aacttgcgtt gaccgcattt ttcttaattt ctaatgatac cttagattta ggaccacgat
     3481 ggattgaccg ttttatcaat ctttctttaa gtgaagcagt acgtgatgtt tgggctaaaa
     3541 aaattattac aggttctggt aaagatgagc caattggatt gcttaaagat ctagatggtg
     3601 cggtcacaag tggtgtatat ccagataaag ctagcgcagg gacattaact tttgcgaaag
     3661 agaagattgt tcttgaactt gcaggggtga tgaaaaaatt atctaaatac acatacaaag
     3721 tagatggtga cgatgaaggt gaagtgaaat accgttctgt tgctggacga gtgtatctaa
     3781 ttgttaatcc tgttgattac tgggatatcg tagctagagt tacatttgca aatttaaaca
     3841 atgtgtatgg gactacgatg ccatttatcg ctgttgatca tatcatcgaa tctgtagacg
     3901 taccagaaag taaatgtatt gcatacgttg ataaagagta tgaagctact caatcaagag
     3961 ctgaaaaagt ctatgtatat aaagaaactt ttgcaatgaa acgcgcaact ttgtatgctg
     4021 tcgacatgct tggtaatggc tatccaacaa ataatgatgc agcacaagta tatgacttgg
     4081 actttacagc gggggagtaa tgtcccctgc aattggtgag ataacaccga cgactgatgg
     4141 ggcagtcatt aatctttcgt aaatggggtt agagtatgaa cactgaagaa tttataaaag
     4201 aatataaggc ccgatttcgt attttccata cttccgaaga tgaggatatc gggaaacagt
     4261 tagagagcgg gtttgccgat ataaaatctc taatcggcag ttttgatcct tccacttatg
     4321 agaaaggaaa agaattagtt tatgaacgta ctcggtatct acggaacgaa tcactagagt
     4381 acttttacga caattttcaa acaatgatta tggatgcatc aattgatctg gcaggtgctg
     4441 gatatgccaa ttaaatcaaa ttataagaag cctaaaattg tagctggtga tctaaatacg
     4501 ccagttactt tttttgagtt tcaacctaat aagggtccag aacctggtga tgaagaaaaa
     4561 aaggaactgt atttttgtac agcactagtt tacaacccat caatgaaaga tcgtgacatt
     4621 cttggcacaa aagaaggcgt tacgataaaa atacgggatc cccatcaatc ttatattcct
     4681 actaacaaac ataaagtaag gatattagat tatcgatatg gagataaagt ttgggaaata
     4741 atcgatgtct caccggattt ggaagaaaat tcaattgtaa agatagtatt agaattaatt
     4801 acttaaattt ggtgataaat gatgagtaac gtaaagtttg aaggcgttca agaaacaatc
     4861 caagctttag aaaaaaagta tggagaaaag aaaactaaga cattaacaaa aaaagcaatc
     4921 aatgttggcg ctgaaaaagt agaaaaacag ttgcaaacgg acatgaccgt ttttaaagac
     4981 aaaggttata cgatcgctga ggttgttcgc aagaacgcta cgtatagaga ttacaaggca
     5041 gaagctgaaa tcggttggaa tggtccacat caacgatata gaatcattca tttgaatgaa
     5101 tggggatata cacgaaaggg caaacaaatt agaccgagag gatttggtgt aataacaaaa
     5161 tctcttaaga attccgagct gttgtacttt gatgctgtag gagaggaggt taaaaagagt
     5221 ttatgaaaga tatgcttaat atcatttacg aggctctttg tttgaatgag tatattcatt
     5281 caatgaccta caacgagaat accgagcaat atcgtattaa attttatgag caacctgaaa
     5341 ccgccgataa aacaggtcca atgatcacaa ttcgtcctgt agatacacct aacgaagcgt
     5401 accacggaag taacaaagag ctttctgtgg agcatatgat acagattgac gttgaatcga
     5461 aatatagagc aacatgcaag cagattcaat atgagataaa aaaagagatg aaaaaactag
     5521 gctttggcca aatttccggt caagggttag atgaatactt tactgaaaca aaccgctttg
     5581 tagacgcgcg tcgttacgaa ggaaatacaa aaatttacga taccgaatat tagaaattaa
     5641 gacacgaaaa taacgtgtct ttttttatta cccaaaatta ggaggaaaat tttatgactt
     5701 tagtaggatt caaaaaaatg acaatcgggg tctttgacag cacagggaag attccagcag
     5761 caaatttata tgtaattgaa ggtgaacaaa acaaaggggc gactgtatct gctgaaatca
     5821 gtgggctatc aaaagaacca tcaaaagttt acggatcgaa catcgcttac tatgtctctc
     5881 aaaaaggaac aggcgatgtt tcagctactt ttggattgtt agatttacca acagaagtaa
     5941 atgacaaaat tcttggttac aaaaccgata caaacaaaat cagcttcttg ggagaagata
     6001 ctgagccacc atattgtgca attttgatgg aatcggagga tttgaacggc gatacagcaa
     6061 tgcttgcgat gttcaaagga aaattcagtc gtgaatctat taatcttaac actacgacaa
     6121 atgaagcttt cgaaccagaa gcagaagaat atgtattttc agcgattgcc aatgatgttg
     6181 aaggtgatgc taaaggccaa acagtcgcaa aatatgttgg agacgaagaa gcttctatta
     6241 ctggtttgaa agagatggta tttcctgcag cgggggagta actagccccg ttgttggagc
     6301 aatcactccc actattaatg gggcaacagt tgaattaagt taggaggata actatgccag
     6361 atacatttaa aatctataaa aaaaatggta ctgaatttac aaaagtcgca gagggtgaaa
     6421 gtccactttc tattactgga attgcagcaa atactcaagt tgctaaagga gattatcaag
     6481 ctacgcgtgt ggtcggaggt caagaatcag taaaagtaga tattcctgca ttcaccactc
     6541 ttccaatttc agttactggt gtgacgttgg ataaaacgac agctgaagtt gaacaaggcg
     6601 gaaccttaaa attaactcct actgtaacac cagcaaatgc aacagacaaa acaggaagct
     6661 ggagcagttc gaatacagct gtagctacgg ttactggagg caacgttaca gtaaaaacag
     6721 acgctgaagt tggcggaact acagaagttt catttactac tacggatggc ggtaaagtag
     6781 caaaatgtac tattacagtt attgaaaaag cagcgggcta gtcttcggac tagctttttt
     6841 atttggaaaa taatggagga atctaaatgg caaaagtaag aattgaattg aaagaagaat
     6901 caggaaagaa agtctatgaa aacatagaca caacagggaa agattatcga aaagctttag
     6961 aaacaatcaa aaagctaaat gaagacggag taatgatttg gaatcgttta gacatttatt
     7021 tggattttgc tgtaggtatc ttcagggata gcaaattgac agctgaacaa attcttgatg
     7081 gcttaccatc tgagaaggta atgagcacat tggacgacat cctaggagaa gtaatgggga
     7141 ttgaaagtaa tccagaccca gacgcaaaaa agtaacaccc gaagaagcag aagaaatgta
     7201 cttaaaccta tgcagagagt tagtgaaagc gggatggtca ctatctgaca tagaagataa
     7261 ttcctttgaa atgatgatga aaattgcgtg cacttcgcct aagaaagaga aacaaaaaga
     7321 agttgatctt aaagacttct tgaaatccat gtagagagga ggtaaacata tggcaaatgg
     7381 aaaaccttta ggcaatatga ttatcaaact agacttagac agttcagctt tttcaaaagg
     7441 tctagccgga gctaaaaacg ctgttaacca tcaaatgaaa gcaatgaaat cacagatgca
     7501 agtaatgaac tcttcaggaa attctctcgg tgctttacaa gcaaaataca atggtttagg
     7561 cggagttcta caagcaaatg aaaagcaagt agaattgctc actaaggctt ataaggatag
     7621 ttttgactct aacggaaacg ctactgcttc gactgctaaa tacgctaatc aattgaatca
     7681 agcaacagct agatcagcaa gttacgaatc tcagatgaaa acaactgttg gacagattgc
     7741 tcggatgaaa gtggaaacgg aagggtttac ggggaaacta aaggctaatt ctgatgtatt
     7801 gattaattct gggaaaaaaa ttgaagcctt tggtaaagga gtttctggga taggatctgc
     7861 cctaactgtt ggagtaacag ctccaatagt ggcaggcgca actgctgtta ctagtgcagc
     7921 tatttcatgg gaaagtgctt ttgcaggtgt aaaaaagaca aatgatgaag ttgtagactc
     7981 gaatggaaac gtagtttatt cctatagcga cttagagaaa ggtctgcgta atcttgccac
     8041 ccaattacct ggtagccatc aagaaatagc aaatgtagcc gaagcagccg ggcaattagg
     8101 tataaagact gaaaacgtag ttagcttcac taaaacgatg atagacatgg gtgaatcgac
     8161 taatatgtcg gctgaaactg cggcaacttc tttagcacga tttgctaata ttacccaaat
     8221 gtctcaaaaa gactttgata aattaggatc agtcattgtt gatctaggta acaattttgc
     8281 tacaaccgaa tcagaaatca ctgaaatggg attgcggtta gcgggagctg gtaaacagat
     8341 tggaatgagc caaggcgaaa ttctaggatt tgctactgct ctatcttctg ttggtgtcga
     8401 agctgaagcg ggtgggtctg ccttttcgaa ggtcatgatc cagatgcaac tagctgtaga
     8461 aaaaggattt ggcgcttttg atcagctgaa acagatggca gaagaacaag gtgttccgtg
     8521 ggttaattta gtaaatgctg tgagagacgg tggaaaatcg ttaaaagcag tatcagagca
     8581 aatgggattc acttcatctg atttaaagaa aatgtataaa gaagcggaca attcgaaaag
     8641 ttcgcttgaa aattttgcga atgtggcagg tgttacaagt gaacaattcc agaagttgtt
     8701 caagagtgat ccttcagaag caatcatcaa gttcattcaa gggctgaaag attcagaaaa
     8761 acatggtact tcagcaatca aagttctgga tgacatggat ataaaagaag ttcgactacg
     8821 cgacagttta ttgagagctg caaatgctag tgacatattc aaaggagcaa ttgaaaaagg
     8881 aaacggcgcg tggaaacaaa atacagcttt aacaaatgaa gctaataaaa gatacgaaac
     8941 aactgaatca aagctaaaga tattgaaaaa cgaagtgatt gatacagcaa tcgatcttgg
     9001 aggtccattt gtagacgcgt tgcgtgatgg tctaaatgta tcgaaaccat tgataaaaaa
     9061 cttagggaat atggcgaaag ctttctcagc tatgagtgat gatcaacaaa gaaacattat
     9121 caaatgggtt ggattagcag cagcagctgg tccagcgctg aagcttttag gaactggtac
     9181 ttcagtaata ggtaaagtta cttcaactac tggaagattg acgaaaagcc ttgttgattt
     9241 agcagcgaaa gcagcagaaa agaaggctat ggatgccttt gcatcgtcgg taacaacagc
     9301 tggtgctgca gctgcaaaga cagcaggagc tggaggtatt ggcgggttag tccctatgat
     9361 gggaaaaaca gctagtgctg caagtactgc tgcgggcgcc agcggaatag gcgcaatgac
     9421 aacgtcacta ggtttgttag gtcctgctct acttggtatt gttggcgttg gtggcgcact
     9481 ggcagttggc tatggagcat ggaaattatt tggtgaagaa gcttggaatt cctcgcaaag
     9541 agtaaagcaa tggggaacag atgtcggtga ggagatagat agtactcttg acggagtaca
     9601 gaagaatatt gaaggtgcaa atggtcaatt cggtcttctc aaagagggtt ttactgttga
     9661 tgatgcaaat aatatggcgg ctaatttcgc aaccgctgga acatctttgg aaacatcttt
     9721 aacgggtaga ataaaggcat tagacgatgc actaaaaggg ctacctcaat cagttcagga
     9781 tgccatgaaa gaagtagctg aatcagaaga agaaacctta gggcaatctc tttctaaaat
     9841 aaaagacaac aatgaagaaa taaaaaaaat cagagaaaga gccgcaaatc agaacagaga
     9901 cttgactgtt tcagaactac aacagataca ggatctaatg aaaggaactt ctcaagcgta
     9961 tgtggatacg ctcgatgttt ctgctaaaga cagaaaaaag atactatcag ccatgaatgg
    10021 tgatgttgca caagctacag aagaagaagc gaagacgtgg ttacaatctt tagcgaaaca
    10081 aaaagctgca actaccgaaa attatagaca aatgcttgaa gaaaaaaagg cggctttaaa
    10141 ggaaaaggga gtaagcaaag aattcattga tgcattagaa gatgaaatga atgattacat
    10201 caaaactaca aatcaaggat tcgatagcca aatagctgct ataacaaata aatatccaca
    10261 attgattaac gaagtgagtt tatcaaatgg tcaattattt agcaaaacgg cagctgctat
    10321 ggacaatact ggtcagtata caaaagtttt tctcgaaagt aatgaaaaga ttttagccca
    10381 agcgaagaaa acatcggatg aaatagcaga taatgccgaa aaaaatgcag aacaacttgg
    10441 tatggtagct gatgaggcta cgcatgctgg atcaacttgg aatggcttag ttttagacga
    10501 aaaaacagga aaagtaaaaa ctaatgtaca agaagtagtg aatgaagcaa cgaaggattt
    10561 cactaaatgg aatgaattga aacctgttgt tcatgatgcg aaactaacaa cgaatgcaaa
    10621 agatgtcatt tccatagcgg cagtacaaaa tggctattgg gatgatatgg aatggaaaga
    10681 aaaagaagca ttattagaag atgagtgttc taaaaatgta tacaaggctt tagaaaatgc
    10741 tggtaaatgg aatgagttag atattccttc taaagaagca atcctaactt caaatactcc
    10801 tgaagttatg ggtgaaactc tcatgaattt gggattatgg gatacttaca aacttcaagt
    10861 gaaagattta gatttgaatg cgtataaagt atacgatgct atcgatggat cagaagaaaa
    10921 attaaatagt tgggctcaat tagataaccc cacaaaagaa ttgttattag ataactcaga
    10981 tttttctaca aaacttttta attccgaaga aatgtttaaa cgttggaatc agttatcatc
    11041 taatgcaaag tttctttcgg ctaataatac taaatttaca gaggttcttt tatcatctga
    11101 cagaatgtgg aatcaatggg taaaccttcc tgataatgag aaaagattac ttgcagataa
    11161 cgaagattta gctaccactg ttttatcttc agaagaaata tacggaagat ggactcaatt
    11221 accagataat caaaaatata tgttagctga caatactgac ttagcaaaca aaatattcgc
    11281 ttcagaagaa tcatggaatg catggaaagc aattccagac acagttaaaa acttgcttgg
    11341 taataatatt gatttactgg ctaaagtgaa agacgggact gtaagtgtag aagaatataa
    11401 caaagttatg ccacttctta aaactctata tggagataac gttaatttaa aagaggcaat
    11461 cagtggggct aaaagctcta tagagaatta caacaaaaat atttttccta gtgataaaac
    11521 tgcagtagga cacgatgagg cttcacaagc tgctaaagat gcatttgaag cgttcaacat
    11581 cttccaaaca aaaataccag ataaaattac taaaactgta tcagctgact ttataggtcc
    11641 tatgccgaaa aatgcgaaag gaacaaactt ccatcctggc ggagcagcta tggtaaacga
    11701 tcaaaaaggg cctacttatg aagagcttat aaccttgcca agcggagaaa cttttatacc
    11761 taaaggacgt aatgtcgttt tagacttacc aagaggttcg aaagtactga acgccactaa
    11821 aactaaacgt ctagtgccta aatatgctga tggtatagga aacataacga cggtttcatc
    11881 tacgctaaac ttagatgctt taattttagc tattaacgag ctaacgacgg tattgagaac
    11941 tcaactgatg gcaggaacga ctgatgctaa agatactaaa ctatcagatg aaacaaaagt
    12001 tgccgatccg attattccgg acagtctttc tgaaaaatca gatcagtatc taggaattgg
    12061 agctcaatgg ttaactaatc taatgaatgg ttggaactca gcggttcctc aatatatgaa
    12121 tagtgaaatg gtttttatta caaactatct caatgcgcta aaacttcaaa acaatcctaa
    12181 ttatacgcaa ggagcaacat ggaataagaa cttactgaat ggatggaata gtttaactgg
    12241 gacctttatt gctacaatca attcattttg taatcaagca atggtcacgc ttagaaacta
    12301 caacactcct atgtacaaca atggccgaac gtggcagcaa aataatctta atggttggaa
    12361 ttcattgtat ggatcattca tagcccgtgt aaatcagctt ggtaacgatt cgattaacaa
    12421 ccttcgttct aaaagtggtg gtttttacaa tgcagggaca ttcttgttgc aatctttaat
    12481 aaacggaatg aattcgttag gtaattctct ttctactaca atgaacaatg tcgctaatac
    12541 aatggttggt ggtataggca aaggagttaa tggcgtcata tcgggagtta actatgtgct
    12601 taaggaagtc gaatcaagta agaatatcgg aaactggcca attccacaat acgctaaggg
    12661 aactgatgga catcctggtg gacttgctat gattaatgat caaaaaggcc tagttcacga
    12721 agaatatgtt cagatgccag atggacgtag ttttattgcc aaaggtagag atcttcttgt
    12781 taatttgcct aaaggtgctc aggtcttaaa tgcttcttta acaaagaaat taaaggaacg
    12841 tttaaatatt ccacactacg aaaaaggtat tggcaattta gatattgttg atttgttaga
    12901 tgatgaaaaa agaatgttag aattcttaac tagcaaagtt gatttttcag gcattaatga
    12961 accatggctt gatatgacta aatcaggaac gtcattaatg tccaaagctg caaatacaat
    13021 gcttcaatca aaattaagtg aattcttcac tcatggcaat tttgatggtg cagtaaatgc
    13081 caatggtgtt tatcaatatt tagttgatgt ggcacagaaa gtaatgggta agttccccgg
    13141 acttacagta acttctggat atcgagcagg agatgcctac tatcacggaa aacggcaagc
    13201 tatcgacttg gcttatccag gtatttctgg agatccgaga tatacagcag cagcaaatta
    13261 cgcttttgaa aaattccctt caaaaattgc gtatgtcatt acgaatggac gtgtacgtga
    13321 ccgcatggga ttatctggaa caaggtcaag cggacaatgg acgaattggc cagatggcga
    13381 tcacttcgat catattcatt taaacggttc gatggggtct ggcgatatat tcaatggagg
    13441 agttggagga agtggtgttg cacgctggcg ttcttatgtg tctaaagcat tgaaaatgaa
    13501 tggattacca gctacagcag cgtatataaa tgcctggatg tcccaaatcc aaacggaatc
    13561 tggtggtaat gagaaagcaa tcggcggcaa tgatgggctc gccgaaggca atgcaacagg
    13621 attacttcaa acgaaacccg gaacttttgc agctaacgct tttcctggtc acggaaatat
    13681 tatgaacgga tttgataaca tgttagcagc aatcaattat gctaagaaac gttacggagt
    13741 tgcaggtatg ttgcaagtta tcgggaaagg acatggatat gccaatggcg gcttgattac
    13801 caaagacgga ctatatcgag caggagaagg aaataaacca gaaatggtta ttccattgac
    13861 aagaaaaaca agagctatcg aacttatggg tcaagcatta gccttccttt ctggaagcaa
    13921 taaaaaagaa acgaaaagtg agattaattc tgtagataat agacatatcc tacaagcgat
    13981 agaaaaacag tctcgtatga caaatgatct gctatcatta ttactctcgt tttttaaaag
    14041 taacaatcga tcagataaag aactagcttt agatattcag aaaattttaa tcaggaggac
    14101 atagtatttg aggacagtaa tattaagaaa taaattgaat gaagaaattg atttgtccac
    14161 agaagattat tttgctactg aaatgtcgaa tctaggtttt gaagttacaa aagagcatat
    14221 tggacaatgg gggagtttca gagaaatagg agagagtatt gaattgtctg aatttcaatc
    14281 atcagttatg atttctgttc acggatttag agaacaacaa ttatatggtt cattagttga
    14341 atttctggct aatggacctt ttgaactaga attttctttt gattcagaaa caatgatcag
    14401 gaaatgtagt ttgaaatcat tatctaaaac agaaattgat tcccaaagct cattgctaac
    14461 agatactttg aatctatatt ttacttcaag ctggtattct attaagcgtg aaaaattaat
    14521 acagaattca acttctctca atacacgtgg aaaagtttac tcatttgttc gaccatatgt
    14581 ctacaccaaa aatgtatggg aaaagaaagg tgtatttaag ttcagtaata attctgtata
    14641 tttgacaaat agtacagagc gaatgtctcc gttaaaaata aggattattg gagagtgctc
    14701 aaatccatat tgggaagtta tccaaaactc taaagtagta gcttcagatg gctattttat
    14761 taatatgaaa gacacacaaa tattagaagt gtctagtttg ctagaagacc aaacggcaat
    14821 tttaaaagat gtaggtggag ttgaatcttc tgtttatcaa caacaagatt ttactaaatc
    14881 aaacttcgtc caagcgcctc taggagaatt cagcgttgtt tttcatgttg gacatgcaaa
    14941 tgtagagatt gaactttatg aggagcgtga tctattttga ttttagccat taccctcttt
    15001 caccgagata tgaatttata taacgaacgt ttaatttcga ctgagttcga atttggaacg
    15061 gatgaaattg gagaagaagt aagtaatttt attacagata aatatgtcca tgtaaaatca
    15121 ggagattttc tactagcaaa atttattcct agcgggaaga ttgcttattt tggagtgatt
    15181 agctcgcaag aagacaaaaa aataaattgc aaaggattac tcagtttggc cgatagcgag
    15241 ataccgactg taaaaatatc aggtgctaat tatgaagagc atattcgacg actgattgaa
    15301 tactacttat tgaacgatcc aacaaaacaa ctaaaaaata ttttggaggt aaaaacagaa
    15361 agcgttactt ctcattctta tcaagctgtt gacacaaata agagacgatt gaatgcatat
    15421 ataataaatg ggttcaaaaa atataatgtt aaatggtatg tcaaagagat taaaggtaaa
    15481 aaaatatata ctggtattcg agcagttgat gattcaattt atattaaaga taattcatct
    15541 gagtttaatg actgggatgt ttttgtgcaa tctcctggac cagggaacga gaacaaatta
    15601 ttgatcgtag ataaagcaat gaaagatata gaaaatccct tggtattgtc tacgtggtat
    15661 ttagatgacg aaaataatct tacgcaagat tcaacgaata agaacattgc attgcctacc
    15721 gttaatttag taaatatata tgatcaaact caagaagata gaccttctta tgaagaggta
    15781 gctaaatcgg agcttaaagg caacgcctat tcacatgaaa ttaaagttaa tgttgtgcta
    15841 aatgctagaa atatcaatgt agaaaatatc gaaacaggaa tgttagcgac tattgtttac
    15901 aaagagcaga cttacaaatc tgttctaact gcctggcgaa tatccagcag taaaaagaat
    15961 atagaattga catttggtaa tgtccgaagt cgttttatgg attattttga agataatggg
    16021 ggataaaaaa tggttagcaa tgtggatgga tatcaatttg aaaacgtgaa agtaagcgca
    16081 gaaaatgatg ctagacttta tcacgtttta tataatcgga aaaatcaggt tattgatggt
    16141 tacgatcagt ctatgaattt atcttcaagc ggattaacag taaaagttgc tgcaggagca
    16201 gcgattattc aaggtcgtat ggtcgttgtt cgacaagaag aaagtataac agttccagca
    16261 aactcaagtg gttatatagc attaacagtg gatttgacac aagaagttat acctggatct
    16321 attcttccag aatcggaaga atatgaatgg actaataatc aagtcaagct agaatttata
    16381 acaaaagtta taaaaggtaa tttaaacaat ggtgataagg tctataattt accactatgc
    16441 tcagttaatt ctactggatc aactgtttca atctcaaaga tatcggatag ttacgagctg
    16501 actctctcta aaggagaaat tttgtggagg gggactgcgt taatgcatga tactcaaact
    16561 gtccaacctt caaaaaaaat ttggcagaca gtttcaggtt ttttgttagt atggctccca
    16621 tacgaaaacg ggcaagcaat tgaggataga tatgtaacta cgccttttta taaggagcgt
    16681 ataactttta ctaatgtttt aggagaaatt gtttcaggat ttgatgacta tcacaaaaag
    16741 tggtttagta aacgaataaa ctataattcg aatactaata tatttacagg tgcagcaagc
    16801 aatgcaagtg gagataacgc aaatatggtg cttaggtata tagtttcttt ttagttagga
    16861 ggtatggaag agtggcaaat ttagaaatta aattatctgc aaataagaga cagccttatc
    16921 tacgtcaccg tgttgttggt agagttggtg atggggggct cacaacaatc aatgtacaac
    16981 ttcttgagga agatgaaatt acaccttttg taattaatct aaacggtact ttgaaatttg
    17041 tgggcgaagt ttcaaacggt aactataccg agggagaacc agaaataatc gattcgacta
    17101 atgggttaat tagttacacg ttcactaagt caaatttcag cacgagtcat caattcaaac
    17161 aagcatattt tgaatatgta gatcctaacg gcaaaaaagt aacttttcag aacttcatca
    17221 tagacgtgtt agaacgagta gatattaatt cggagcaagc gaaatactat atttcttcat
    17281 tggaaaaatt acagagtgaa atgcaaacca ctttcaatca gttcattagt gataaacagg
    17341 tccaatacga tcaaatctac tcgaaatata acgaattagt aagattgata aatgaatcag
    17401 ataagcaagt aaatgatcgt attgataagg caaataatcg tattgatcaa accaatcagc
    17461 aaatcggcga tctcggcaag ctgaaaaaga tgtacagtaa cagcattga
//
LOCUS       DACDCV010000060        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDCV010000060 DACDCV010000000
VERSION     DACDCV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.060"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IV041_001892"
                     /pseudo
     CDS             1..368
                     /locus_tag="IV041_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IV041_001893"
     CDS             548..1489
                     /locus_tag="IV041_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8382656.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IV041_001894"
     CDS             1558..2289
                     /locus_tag="IV041_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8382657.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IV041_001895"
     CDS             2433..4613
                     /locus_tag="IV041_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8382658.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IV041_001896"
     CDS             4633..5115
                     /locus_tag="IV041_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8382659.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IV041_001897"
     CDS             5131..5892
                     /locus_tag="IV041_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8382660.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IV041_001898"
     CDS             6023..7357
                     /locus_tag="IV041_001898"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8382661.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IV041_001899"
     CDS             7785..8270
                     /locus_tag="IV041_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8382662.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IV041_001900"
     CDS             8263..8946
                     /locus_tag="IV041_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8382663.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IV041_001901"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IV041_001901"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8382664.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IV041_001902"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IV041_001902"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8382665.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IV041_001903"
     CDS             12562..13191
                     /locus_tag="IV041_001903"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8382666.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IV041_001904"
     CDS             13243..13575
                     /locus_tag="IV041_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8382667.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IV041_001905"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IV041_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8382668.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IV041_001906"
     CDS             14401..15774
                     /locus_tag="IV041_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8382669.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IV041_001907"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IV041_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8382670.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IV041_001908"
     CDS             16829..17269
                     /locus_tag="IV041_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8382671.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDCV010000061        16911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDCV010000061 DACDCV010000000
VERSION     DACDCV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.061"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV041_001909"
     CDS             complement(<1..258)
                     /locus_tag="IV041_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382672.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IV041_001910"
     CDS             complement(274..924)
                     /locus_tag="IV041_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382673.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IV041_001911"
     CDS             complement(935..2107)
                     /locus_tag="IV041_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8382674.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IV041_001912"
     CDS             complement(2126..2998)
                     /locus_tag="IV041_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8382675.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IV041_001913"
     CDS             complement(3020..3940)
                     /locus_tag="IV041_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382676.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IV041_001914"
     CDS             complement(3940..4209)
                     /locus_tag="IV041_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382677.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IV041_001915"
     CDS             complement(4206..4586)
                     /locus_tag="IV041_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8382678.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="IV041_001916"
     CDS             complement(5053..6516)
                     /locus_tag="IV041_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8382679.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="IV041_001917"
     CDS             complement(6580..9597)
                     /locus_tag="IV041_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8382680.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="IV041_001918"
     CDS             complement(9748..10587)
                     /locus_tag="IV041_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8382681.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="IV041_001919"
     CDS             10732..10977
                     /locus_tag="IV041_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382682.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="IV041_001920"
     CDS             11040..11462
                     /locus_tag="IV041_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382683.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="IV041_001921"
     CDS             complement(11556..11873)
                     /locus_tag="IV041_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382684.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="IV041_001922"
     CDS             complement(11900..12106)
                     /locus_tag="IV041_001922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382685.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="IV041_001923"
     CDS             complement(12087..12269)
                     /locus_tag="IV041_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382686.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="IV041_001924"
     CDS             complement(12283..12633)
                     /locus_tag="IV041_001924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382687.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="IV041_001925"
     CDS             complement(12659..12961)
                     /locus_tag="IV041_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382688.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="IV041_001926"
     CDS             complement(12964..13197)
                     /locus_tag="IV041_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382689.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="IV041_001927"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="IV041_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="IV041_001928"
     CDS             complement(14628..15743)
                     /locus_tag="IV041_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382690.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="IV041_001929"
     CDS             complement(15749..16147)
                     /locus_tag="IV041_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382691.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4516 a         3511 c         2639 g         6245 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc t
//
LOCUS       DACDCV010000062        16375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDCV010000062 DACDCV010000000
VERSION     DACDCV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.062"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IV041_001930"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IV041_001930"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8382692.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IV041_001931"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IV041_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8382693.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IV041_001932"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IV041_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8382694.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IV041_001933"
     CDS             2855..3730
                     /locus_tag="IV041_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8382695.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IV041_001934"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IV041_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8382696.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IV041_001935"
     CDS             4627..5061
                     /locus_tag="IV041_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8382697.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IV041_001936"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IV041_001936"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8382698.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IV041_001937"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IV041_001937"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8382699.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IV041_001938"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IV041_001938"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8382700.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IV041_001939"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IV041_001939"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8382701.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IV041_001940"
     CDS             13332..14258
                     /locus_tag="IV041_001940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8382702.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IV041_001941"
     CDS             14440..15333
                     /locus_tag="IV041_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8382703.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IV041_001942"
     CDS             15467..15592
                     /locus_tag="IV041_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382704.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IV041_001943"
     CDS             complement(15726..16358)
                     /locus_tag="IV041_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382705.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5816 a         2791 c         3492 g         4276 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaa
//
LOCUS       DACDCV010000063        16152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDCV010000063 DACDCV010000000
VERSION     DACDCV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.063"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1250
                     /locus_tag="IV041_001944"
     CDS             <1..1250
                     /locus_tag="IV041_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8382706.1"
                     /translation="GLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSV
                     RIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELK
                     QAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMK
                     SVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQ
                     LLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLL
                     LKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1633..2361)
                     /locus_tag="IV041_001945"
     CDS             complement(1633..2361)
                     /locus_tag="IV041_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382707.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2339..3913)
                     /locus_tag="IV041_001946"
     CDS             complement(2339..3913)
                     /locus_tag="IV041_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8382708.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4142..5080
                     /locus_tag="IV041_001947"
     CDS             4142..5080
                     /locus_tag="IV041_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382709.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5092..6003
                     /locus_tag="IV041_001948"
     CDS             5092..6003
                     /locus_tag="IV041_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382710.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6027..7499
                     /locus_tag="IV041_001949"
     CDS             6027..7499
                     /locus_tag="IV041_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8382711.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7550..8161
                     /locus_tag="IV041_001950"
     CDS             7550..8161
                     /locus_tag="IV041_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8382712.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8191..10104
                     /locus_tag="IV041_001951"
     CDS             8191..10104
                     /locus_tag="IV041_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8382713.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10232..13213
                     /locus_tag="IV041_001952"
     CDS             10232..13213
                     /locus_tag="IV041_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8382714.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13210..14871
                     /locus_tag="IV041_001953"
     CDS             13210..14871
                     /locus_tag="IV041_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8382715.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14911..15843
                     /locus_tag="IV041_001954"
     CDS             14911..15843
                     /locus_tag="IV041_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8382716.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5237 a         2612 c         3235 g         5068 t
ORIGIN      
        1 tcggattaac agataaacat ttattttttg atgaagaatg gctgattgaa aaagaatata
       61 aaggcgttca agcgcagttt attaaaggaa aattggatga ctctccttcc tattgtgaac
      121 actgtcgatc ggttcgtatt atccgcaacg gttcctatac gacacgcact cagatattga
      181 aagtcaaaga aaagttaact attttagaat taaaacgtac ccgttttctc tgccatgatt
      241 gtggaaggac cttttctgcg aaaacggact tagttgatga acatcatcaa ctaacaaagg
      301 aactgaagca ggcaattctc atggaactgt acgaaaatca atcacgtaaa ctaatcgcta
      361 agaagtactt cgtctcggat gggacggtga cacggatctt gcgtgaagca acgaagcatt
      421 accagtcacg tatgaacttc cttccgacgg tgttgtgtat ggatgagttc aaatcaatga
      481 agtctgtttc tggttccatg agttttattt gtgttgatgg gacaacccat cagctattta
      541 cgatccttga agatcgcaga ctttacaaac taactcaata cttcctgcac tttcctagaa
      601 aagcccgttt aaaggtcaag tacctagtga tggacatgaa tgccagctat tgtcagttgc
      661 ttaaaacagt ctttccaaat gcggagatcg taactgaccg ctttcatatt gtccaacaca
      721 tcaatcgcag cttcaatcaa ttacgtgtcc agatcatgaa tcgttttcga acctcacact
      781 ctgaaaatca gaagaagtac cgtcggctca agcgatattg gaaactctta ctcaaagatt
      841 ccacgacatg ggagccactc aaacggcatt atcatcgact gtttaaacga ccaattagtc
      901 aaacagaaat cgttgatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt
      961 tttgtcaatt attacgctat tatttcgtta aacgaggtac ggaaggcttt caagaaaccc
     1021 ttaaaacaat ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatt
     1081 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
     1141 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
     1201 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
     1261 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
     1321 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
     1381 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
     1441 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
     1501 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
     1561 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
     1621 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
     1681 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
     1741 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
     1801 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
     1861 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
     1921 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
     1981 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
     2041 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     2101 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     2161 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     2221 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     2281 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     2341 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     2401 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     2461 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     2521 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     2581 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     2641 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     2701 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     2761 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     2821 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     2881 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     2941 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     3001 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     3061 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     3121 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     3181 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     3241 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     3301 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     3361 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     3421 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     3481 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     3541 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     3601 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     3661 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     3721 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     3781 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     3841 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     3901 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     3961 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     4021 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     4081 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     4141 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     4201 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     4261 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     4321 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     4381 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     4441 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     4501 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     4561 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     4621 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     4681 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     4741 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     4801 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     4861 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     4921 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     4981 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     5041 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     5101 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     5161 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     5221 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     5281 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     5341 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     5401 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     5461 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     5521 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     5581 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     5641 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     5701 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     5761 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     5821 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     5881 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     5941 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     6001 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     6061 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     6121 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     6181 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     6241 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     6301 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     6361 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     6421 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     6481 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     6541 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     6601 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     6661 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     6721 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     6781 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     6841 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     6901 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     6961 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     7021 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     7081 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     7141 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     7201 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     7261 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     7321 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     7381 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     7441 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     7501 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     7561 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     7621 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     7681 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     7741 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     7801 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     7861 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     7921 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     7981 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     8041 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     8101 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     8161 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     8221 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     8281 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     8341 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     8401 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     8461 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     8521 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     8581 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     8641 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     8701 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     8761 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     8821 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     8881 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     8941 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     9001 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     9061 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     9121 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     9181 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     9241 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     9301 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     9361 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     9421 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     9481 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     9541 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     9601 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     9661 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     9721 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     9781 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     9841 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     9901 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     9961 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
    10021 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
    10081 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
    10141 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
    10201 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
    10261 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
    10321 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
    10381 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
    10441 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
    10501 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
    10561 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
    10621 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
    10681 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
    10741 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
    10801 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
    10861 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
    10921 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
    10981 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
    11041 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    11101 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    11161 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    11221 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    11281 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    11341 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    11401 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    11461 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    11521 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    11581 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    11641 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    11701 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    11761 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    11821 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    11881 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    11941 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    12001 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    12061 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    12121 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    12181 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    12241 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    12301 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    12361 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    12421 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    12481 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    12541 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    12601 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    12661 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    12721 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    12781 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    12841 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    12901 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    12961 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    13021 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    13081 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    13141 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    13201 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    13261 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    13321 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    13381 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    13441 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    13501 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    13561 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    13621 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    13681 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    13741 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    13801 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    13861 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    13921 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    13981 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    14041 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    14101 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    14161 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    14221 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    14281 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    14341 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    14401 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    14461 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    14521 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    14581 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    14641 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    14701 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    14761 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    14821 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    14881 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    14941 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    15001 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    15061 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    15121 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    15181 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    15241 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    15301 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    15361 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    15421 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    15481 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    15541 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    15601 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    15661 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    15721 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    15781 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    15841 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    15901 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    15961 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    16021 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    16081 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    16141 catttcaaaa gt
//
LOCUS       DACDCV010000064        15949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDCV010000064 DACDCV010000000
VERSION     DACDCV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.064"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(388..810)
                     /locus_tag="IV041_001955"
     CDS             complement(388..810)
                     /locus_tag="IV041_001955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8382717.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(824..2230)
                     /gene="atpD"
                     /locus_tag="IV041_001956"
     CDS             complement(824..2230)
                     /gene="atpD"
                     /locus_tag="IV041_001956"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8382718.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2253..3155)
                     /locus_tag="IV041_001957"
     CDS             complement(2253..3155)
                     /locus_tag="IV041_001957"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8382719.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3169..4725)
                     /locus_tag="IV041_001958"
     CDS             complement(3169..4725)
                     /locus_tag="IV041_001958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8382720.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4748..5290)
                     /locus_tag="IV041_001959"
     CDS             complement(4748..5290)
                     /locus_tag="IV041_001959"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8382721.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="IV041_001960"
     CDS             complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="IV041_001960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8382722.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="IV041_001961"
     CDS             complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="IV041_001961"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8382723.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="IV041_001962"
     CDS             complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="IV041_001962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8382724.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="IV041_001963"
                     /pseudo
     CDS             complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="IV041_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10027..10773)
                     /locus_tag="IV041_001964"
     CDS             complement(10027..10773)
                     /locus_tag="IV041_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8382725.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10885..11121)
                     /gene="secG"
                     /locus_tag="IV041_001965"
     CDS             complement(10885..11121)
                     /gene="secG"
                     /locus_tag="IV041_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8382726.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11239..11517)
                     /locus_tag="IV041_001966"
     CDS             complement(11239..11517)
                     /locus_tag="IV041_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8382727.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11630..12598)
                     /locus_tag="IV041_001967"
     CDS             complement(11630..12598)
                     /locus_tag="IV041_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8382728.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12813..12857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12881..13855
                     /locus_tag="IV041_001968"
     CDS             12881..13855
                     /locus_tag="IV041_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8382729.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13860..14435
                     /locus_tag="IV041_001969"
     CDS             13860..14435
                     /locus_tag="IV041_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8382730.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14502..15641)
                     /locus_tag="IV041_001970"
     CDS             complement(14502..15641)
                     /locus_tag="IV041_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8382731.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3443 c         2856 g         5294 t
ORIGIN      
        1 tctgagccag aatgtggttc attctgactc agatttgatt ttgcacaact catcagtcct
       61 tattgacaaa gagcccaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa
      121 tcatctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt
      181 gtttctctaa tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag
      241 aagtagcttc ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta
      301 aattccttct tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta
      361 aacacggata aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat
      421 tgctcggtgc aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc
      481 ttcttcgatt tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc
      541 acgttcacgt tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat
      601 tccgccatta acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac
      661 ttcatcaatc gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc
      721 agctgtcgta cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt
      781 taccacattt actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt
      841 tcgctttttc tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat
      901 catcgtattt gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat
      961 atgaacctgg aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt
     1021 ggactctgcg ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta
     1081 agatagcaat gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg
     1141 ctactttata atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca
     1201 atggatctac cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg
     1261 catccaaatg ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat
     1321 agattgcttg gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt
     1381 gacccatttc tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca
     1441 aggctgatac ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta
     1501 catcttgacc ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac
     1561 gcatacgtgc acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa
     1621 ctcctgaatc tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac
     1681 cggtaaatac agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca
     1741 gtacggtttt accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg
     1801 gggcaagcaa atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt
     1861 cgtcaaaggc tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg
     1921 tttctaaatc gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta
     1981 caggaacaga gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg
     2041 ttgattccat cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa
     2101 caacttttga tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta
     2161 aggattggtc taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc
     2221 ctgaactcat cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca
     2281 atttcggtaa tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc
     2341 tcatcaatga tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca
     2401 tgttctgctg ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc
     2461 aataactgag acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta
     2521 gttgcttctt ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta
     2581 gttagcgaat tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg
     2641 tacatagttg ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat
     2701 agattgcgca gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt
     2761 ccgccaattg cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac
     2821 atcgtttgtt tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg
     2881 acgatataac ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc
     2941 cctctaggat tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt
     3001 tctcttactt ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac
     3061 ttagaagcag acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg
     3121 atacgtgtct tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg
     3181 aatggatttc aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc
     3241 tttatattcc tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg
     3301 gattgtttca aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa
     3361 gtctaaaata ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat
     3421 cactacttgt ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt
     3481 acggcgtcca cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt
     3541 aaatgcttct aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt
     3601 cattgctttg atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc
     3661 tggacgagta cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga
     3721 aataacgttt gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa
     3781 ggcagtcatt gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg
     3841 tgagtgtaag tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag
     3901 caaggaaagt tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac
     3961 atgtttcccg ttgtacatga actcttcacc cattgcagta ccagcatatg gtgcgatata
     4021 aagcaacggc gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc
     4081 atattttttc aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac
     4141 atagatacag atcatatctt gacctttttg gttgatgatc gtatcgatcg caatggaagt
     4201 tttccctgtt ttacggtcac cgataactaa ttcacgttgg ccgcgtccga ttggtacaag
     4261 agcgtcgatt gcttttagac ctgtttgcat tggttcgtta acagatttac gttgcataac
     4321 gcctggtgcc attgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc
     4381 aattggctga cctagcggat taactacccg tccaatcaag gcctctccaa ctggaacttc
     4441 catgatcttt cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag
     4501 gataatgata cctacatcat ttgattctaa gttttgcgcc attccgtatg agccatttga
     4561 aaattcaagc aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc
     4621 acctacataa gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat
     4681 ttgttcttta atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct
     4741 ctttgtacta tctatttaat tgattacgca tattttctaa ttgcgtgcga atgcttccgt
     4801 cgattactcg atggttagct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt
     4861 gttttaattc gactgattga tagttcattg tttttgcaac attcttttct aattgtattc
     4921 gttgttcgtc tgaaagtgga actgctgttg tgacacttcc caacagcaaa ccttgatgtt
     4981 catcatatcg acgttcatat tcttcgatca tgaacaaaag atcatccatt cggttatacg
     5041 cataaacaac ttctaaaaag tttttaacga ttccatcata gccactaaca agcttgtcca
     5101 tgatttctcg tttttcatga ggttctagac gaacatcact taacatatta cctaatccag
     5161 gtacttgttc atagatttga cgtaagctta cgagatcttg ataaatttct tctgctttat
     5221 ttgaatcgat cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat
     5281 ctagtttcat ttgaagaacc tagaccttcg atatattgat tgattaatga ttcgtgcatt
     5341 tctggagata attctttgtt caagattttt tccgcgattt gaagagatag ttctgcaaca
     5401 tcgtctttta cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggcttta
     5461 ctttttagac gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct
     5521 ccactttctt tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt
     5581 tgttcgcgtt cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatccaaa
     5641 tcattggcga tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa
     5701 tgcttcaaga gagccagtag tatcaaaaat gagccgctga caacgatgac atttcctaac
     5761 atcgcattgc caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt
     5821 tgaagtactt ctagttttca tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa
     5881 tactgattca attaaacacc aaaaactaga atcaaggcga caactacccc taagatagga
     5941 acagcttcga ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca
     6001 ggttggcgag ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct
     6061 ccgattgctg ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc
     6121 ctaaattaga taaatagtag tttgttttta aaacgaatat tattcttcca cttcaatctt
     6181 atggctcata taaaccatag ataaagtaac gaagacaaat gcttggatgc ttccgataaa
     6241 caaggagaat gcgatccaga ccatctctaa tggaattgct aacggcaatg agaaccagcc
     6301 taagcttatt accatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc
     6361 ataaagacgt aaagcaagtg ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa
     6421 cataaaacta tatggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt
     6481 caccccaaat aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg
     6541 gtctgctgtc gggcttttcc aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa
     6601 accgatgttg tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt
     6661 gctcaattct tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca
     6721 ttctatcgca ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc
     6781 taagaagaaa acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca
     6841 aattggtcca atgtggaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc
     6901 gaaattcaca taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca
     6961 cacggtatca taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact
     7021 tttttatttc agtcaaaaac gaagcgaatt tttctttata cacggtgctc ctgatctaag
     7081 aaaaaaccta tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt
     7141 aaaagcaaaa gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta
     7201 atgttcgatc aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcataag
     7261 attttttccc tttcgccaga ccaatcaata ccttggcata gccgttacga atatagactt
     7321 ttaaaggaac aacagtgatt ccagtatttt tcaattcatt ttcaagccgg atgatttgtt
     7381 ttttgtgtaa taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt
     7441 cataaggact gatatgaaca ttatgcaaga aagcttctcc gttgcggacg cgaataaatc
     7501 catcttttag attgatccgg ctgtttcgta tcgacttgat ctcggttcct tgcaagacca
     7561 tccctgcttc catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa
     7621 ttaattttcc ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt
     7681 tcttcttttt aacacctttg taaaatggct tcttcccttt tttcttaccg aacgtttgct
     7741 tactatcgct atatcgtttg ttcttcttgc catttctttc tgggcgtttt cctgcttgtt
     7801 ttccttcgcg ttttcctgat tttccatttc gtgtttttgg ccgttccaac gctgtaacct
     7861 cttctgcgga aacaagttca aaatctactg ctcgttcatc tggatcagct ttgactactt
     7921 ttactcgcac tttctgtccg attttaaaag tcagaccagt tctttcacct acaagagcta
     7981 aatgattttc aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatccct
     8041 ctactgtatt cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat
     8101 cgaattcttc ccccactttg tccatcatga attctgcttt cttcatggaa tctacttcac
     8161 gttctgtttc tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt
     8221 ccgtagccca cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac
     8281 gatggacgat tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg
     8341 ccgctaagcc ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta
     8401 gcaacatagt attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct
     8461 gtaaatcttt tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag
     8521 caaaatcaaa gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa
     8581 acggcagatt taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag
     8641 attcgatcaa gcgttcacca attcctctgc ttcttagctg gatatcattt gggtgtcctt
     8701 cagcatcaac aaggattttt gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc
     8761 gcatcgtttc aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag
     8821 catagcgttg catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg
     8881 tcatccgttc tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat
     8941 ttggatcaat ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac
     9001 agataccatt cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac
     9061 ttgtccctct ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa
     9121 catctgcaat gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg
     9181 catcatctag atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat
     9241 ctctacgtcc agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca
     9301 atacatcatc aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca
     9361 tgccagggtc attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat
     9421 accctttttc agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa
     9481 ctccctctgc tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa
     9541 cgccgtaaag atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta
     9601 actgagtagt cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat
     9661 ctgtatttgc tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag
     9721 tcgcttcttt tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc
     9781 ctcgttcatt tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt
     9841 ttcctttttt attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta
     9901 gtaatttaaa atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa
     9961 aactgcgttt gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg
    10021 tcatatttat tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa
    10081 ttcgcgtctt gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt
    10141 cactcgctga cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc
    10201 atctttccca gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac
    10261 gattgctgcc tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac
    10321 tgctttttca ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta
    10381 tttttcaaag ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc
    10441 gccgccaatc actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa
    10501 tcctaatacg gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt
    10561 gtctgcccac cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg
    10621 tcctttaaaa agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat
    10681 tcgaacatcg tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg
    10741 ttttgcataa aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta
    10801 ccatagtttc tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg
    10861 ccgttttgcg agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa
    10921 gatcatccag atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg
    10981 agctttttgt ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt
    11041 ttgcttgctt ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat
    11101 tcccaaaatt aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact
    11161 ttatcataga taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa
    11221 aactttcagc tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc
    11281 aaactctttt tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa
    11341 ttctactcca gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac
    11401 aaatgtatgg tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat
    11461 ctcttgttgt tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg
    11521 ccgcactcct caaataactg atattttttt attcctacct tattttagca gtctcctgat
    11581 ctataaacaa ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg
    11641 ttattctttg ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg
    11701 atgcttccaa gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat
    11761 tttgaatcgt gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca
    11821 ataaaggcag atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac
    11881 cggcgtattt ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag
    11941 tgattttact taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa
    12001 taaatccttt gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc
    12061 ccattgatcc gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga
    12121 tccaggcaat taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt
    12181 ctccgtgcgc acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac
    12241 ctatatgaga catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt
    12301 tattctgctt atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt
    12361 tgattgtttt gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc
    12421 gttttttttc tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat
    12481 accagtcata tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa
    12541 gaaaatggta actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg
    12601 tacacccgcc gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag
    12661 gattctttat tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa
    12721 gttctcctca atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata
    12781 ctaatttcgt gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa
    12841 taaaaaacta agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga
    12901 ttgcgatcca gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat
    12961 cgaagtagtt gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa
    13021 tgtgctgaaa tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga
    13081 aaaacctttg aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg
    13141 ggaaacaaat ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt
    13201 tttggctgat tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac
    13261 caatatcgct tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt
    13321 ctcgatgggt ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta
    13381 ctggtgtgat cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat
    13441 ggttgggtta gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg
    13501 tcaaatgaat cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt
    13561 ccactggaaa caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta
    13621 ttttatcgag ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg
    13681 aataagtcgt ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag
    13741 taaaattatg acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt
    13801 aaatgcacaa gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa
    13861 tgaataaaaa caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca
    13921 attatattgg aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg
    13981 gaacgatttt tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca
    14041 gcggtatttt aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa
    14101 ttctcacagg cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca
    14161 gcttgaaact gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga
    14221 tcattaccgt tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc
    14281 tattacatgg gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag
    14341 actaccttga tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac
    14401 aacgcttgat atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat
    14461 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
    14521 ataattcctt gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct
    14581 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
    14641 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
    14701 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
    14761 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
    14821 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
    14881 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
    14941 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
    15001 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
    15061 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
    15121 tgcgtataca tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct
    15181 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
    15241 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
    15301 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
    15361 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
    15421 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
    15481 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
    15541 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
    15601 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
    15661 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
    15721 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
    15781 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
    15841 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
    15901 aacatcatat acagaagcta gttgcaaaaa aaactctcta cctgctgat
//
LOCUS       DACDCV010000065        15725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDCV010000065 DACDCV010000000
VERSION     DACDCV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.065"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IV041_001971"
     CDS             complement(26..466)
                     /locus_tag="IV041_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8382732.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IV041_001972"
     CDS             626..1132
                     /locus_tag="IV041_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8382733.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IV041_001973"
     CDS             1300..3213
                     /locus_tag="IV041_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8382734.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IV041_001974"
     CDS             3218..4219
                     /locus_tag="IV041_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8382735.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IV041_001975"
     CDS             complement(4421..4627)
                     /locus_tag="IV041_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8382736.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5273..6121)
                     /locus_tag="IV041_001976"
     CDS             complement(5273..6121)
                     /locus_tag="IV041_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8382737.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6285..7187
                     /gene="gnd"
                     /locus_tag="IV041_001977"
     CDS             6285..7187
                     /gene="gnd"
                     /locus_tag="IV041_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8382738.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7189..8190
                     /locus_tag="IV041_001978"
     CDS             7189..8190
                     /locus_tag="IV041_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8382739.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8187..8471
                     /locus_tag="IV041_001979"
     CDS             8187..8471
                     /locus_tag="IV041_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8382740.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8504..9301
                     /locus_tag="IV041_001980"
     CDS             8504..9301
                     /locus_tag="IV041_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8382741.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9315..10148
                     /locus_tag="IV041_001981"
     CDS             9315..10148
                     /locus_tag="IV041_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382742.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10166..11023
                     /locus_tag="IV041_001982"
     CDS             10166..11023
                     /locus_tag="IV041_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8382743.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11045..11554
                     /locus_tag="IV041_001983"
     CDS             11045..11554
                     /locus_tag="IV041_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8382744.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11591..11992
                     /locus_tag="IV041_001984"
     CDS             11591..11992
                     /locus_tag="IV041_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382745.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12104..12457
                     /locus_tag="IV041_001985"
     CDS             12104..12457
                     /locus_tag="IV041_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382746.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12491..12934
                     /locus_tag="IV041_001986"
     CDS             12491..12934
                     /locus_tag="IV041_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8382747.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12951..14036
                     /gene="uxuA"
                     /locus_tag="IV041_001987"
     CDS             12951..14036
                     /gene="uxuA"
                     /locus_tag="IV041_001987"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8382748.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14029..14742
                     /locus_tag="IV041_001988"
     CDS             14029..14742
                     /locus_tag="IV041_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8382749.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15494..15694
                     /locus_tag="IV041_001989"
     CDS             15494..15694
                     /locus_tag="IV041_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8382750.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         5442 a         2460 c         3321 g         4502 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
     4981 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
     5041 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
     5101 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
     5161 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
     5221 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
     5281 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
     5341 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
     5401 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
     5461 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
     5521 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
     5581 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
     5641 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
     5701 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
     5761 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
     5821 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
     5881 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
     5941 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
     6001 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
     6061 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
     6121 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
     6181 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
     6241 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
     6301 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
     6361 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
     6421 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
     6481 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
     6541 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
     6601 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
     6661 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
     6721 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
     6781 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
     6841 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
     6901 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
     6961 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
     7021 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
     7081 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
     7141 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
     7201 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
     7261 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
     7321 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
     7381 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
     7441 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
     7501 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
     7561 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
     7621 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
     7681 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
     7741 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
     7801 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
     7861 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
     7921 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
     7981 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
     8041 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
     8101 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
     8161 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
     8221 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
     8281 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
     8341 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
     8401 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
     8461 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
     8521 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
     8581 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
     8641 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
     8701 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
     8761 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
     8821 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
     8881 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
     8941 attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa
     9001 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
     9061 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
     9121 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
     9181 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
     9241 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
     9301 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
     9361 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
     9421 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
     9481 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
     9541 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
     9601 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
     9661 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
     9721 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
     9781 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
     9841 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
     9901 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
     9961 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    10021 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    10081 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    10141 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    10201 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    10261 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    10321 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    10381 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    10441 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    10501 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    10561 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    10621 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    10681 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac
    10741 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    10801 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    10861 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    10921 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    10981 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    11041 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    11101 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    11161 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    11221 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    11281 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    11341 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    11401 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    11461 acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    11521 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    11581 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    11641 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    11701 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    11761 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    11821 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    11881 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    11941 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    12001 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    12061 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    12121 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    12181 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    12241 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    12301 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    12361 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    12421 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    12481 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    12541 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    12601 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    12661 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    12721 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    12781 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    12841 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    12901 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    12961 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    13021 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    13081 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    13141 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    13201 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    13261 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    13321 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    13381 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    13441 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    13501 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    13561 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    13621 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    13681 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    13741 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    13801 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    13861 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    13921 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    13981 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    14041 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    14101 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    14161 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    14221 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    14281 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    14341 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    14401 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    14461 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    14521 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    14581 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    14641 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    14701 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    14761 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    14821 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    14881 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    14941 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    15001 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    15061 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    15121 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    15181 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    15241 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    15301 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    15361 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    15421 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    15481 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    15541 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    15601 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    15661 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    15721 cgaaa
//
LOCUS       DACDCV010000066        15614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDCV010000066 DACDCV010000000
VERSION     DACDCV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.066"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IV041_001990"
     CDS             138..1475
                     /locus_tag="IV041_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8382751.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IV041_001991"
     CDS             1523..3652
                     /locus_tag="IV041_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8382752.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IV041_001992"
     CDS             3627..4316
                     /locus_tag="IV041_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382753.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IV041_001993"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IV041_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IV041_001994"
     CDS             complement(5546..5740)
                     /locus_tag="IV041_001994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382754.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IV041_001995"
     CDS             complement(5790..6230)
                     /locus_tag="IV041_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8382755.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IV041_001996"
     CDS             complement(6234..7079)
                     /locus_tag="IV041_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8382756.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IV041_001997"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IV041_001997"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8382757.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IV041_001998"
     CDS             complement(8703..9650)
                     /locus_tag="IV041_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8382758.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IV041_001999"
     CDS             complement(9873..10223)
                     /locus_tag="IV041_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8382759.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IV041_002000"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IV041_002000"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8382760.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IV041_002001"
     CDS             11646..11966
                     /locus_tag="IV041_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8382761.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IV041_002002"
     CDS             11988..12452
                     /locus_tag="IV041_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8382762.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IV041_002003"
     CDS             12657..13262
                     /locus_tag="IV041_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8382763.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IV041_002004"
     CDS             complement(13301..14590)
                     /locus_tag="IV041_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8382764.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IV041_002005"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IV041_002005"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8382765.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4880 a         2908 c         3200 g         4626 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaag
//
LOCUS       DACDCV010000067        15352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDCV010000067 DACDCV010000000
VERSION     DACDCV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.067"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1145
                     /locus_tag="IV041_002006"
     CDS             <1..1145
                     /locus_tag="IV041_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382766.1"
                     /translation="KRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKRAEKIRDEFL
                     LELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHNRIYKFFYPK
                     KIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVR
                     KPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLN
                     KKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILL
                     GDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDS
                     GQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1719..2288
                     /locus_tag="IV041_002007"
     CDS             1719..2288
                     /locus_tag="IV041_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8382767.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2275..3135
                     /locus_tag="IV041_002008"
     CDS             2275..3135
                     /locus_tag="IV041_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8382768.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3325..4029
                     /locus_tag="IV041_002009"
     CDS             3325..4029
                     /locus_tag="IV041_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8382769.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4034..5869
                     /gene="walK"
                     /locus_tag="IV041_002010"
     CDS             4034..5869
                     /gene="walK"
                     /locus_tag="IV041_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8382770.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5866..7182
                     /locus_tag="IV041_002011"
     CDS             5866..7182
                     /locus_tag="IV041_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382771.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7183..8052
                     /locus_tag="IV041_002012"
     CDS             7183..8052
                     /locus_tag="IV041_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382772.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8107..8916
                     /locus_tag="IV041_002013"
     CDS             8107..8916
                     /locus_tag="IV041_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8382773.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9019..9648)
                     /locus_tag="IV041_002014"
     CDS             complement(9019..9648)
                     /locus_tag="IV041_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8382774.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9818..11110
                     /locus_tag="IV041_002015"
     CDS             9818..11110
                     /locus_tag="IV041_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382775.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11186..11848)
                     /locus_tag="IV041_002016"
     CDS             complement(11186..11848)
                     /locus_tag="IV041_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8382776.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12073..12357
                     /locus_tag="IV041_002017"
     CDS             12073..12357
                     /locus_tag="IV041_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8382777.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12408..14033
                     /gene="groL"
                     /locus_tag="IV041_002018"
     CDS             12408..14033
                     /gene="groL"
                     /locus_tag="IV041_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8382778.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14122..15270)
                     /locus_tag="IV041_002019"
     CDS             complement(14122..15270)
                     /locus_tag="IV041_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382779.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
BASE COUNT         5109 a         2586 c         3198 g         4459 t
ORIGIN      
        1 aaaaaagagg aacattacaa gtagttgtat cttataaaga ctctaacgga aaaattagac
       61 aaaagtggaa agatacaaaa ttaaaaaaga aaggaaataa aaaacgagca gaaaaaatta
      121 gagatgaatt tttactagaa ttggaaagtc aattagctat ggaatgtgaa tgtgctaatc
      181 caaatttatt gttttatgac tatttgctta attatttgga agtatcaaaa aaacaagtag
      241 catttaacac atatgttggg tataagcatt acgtccataa tcgaatttac aagttttttt
      301 atcctaaaaa aattaaacta aataggttaa agccttatca tttacaagag ttttatcaat
      361 atatgttaaa tgataactgt acagcaaata cggttattca ttatcacgca tttatacgca
      421 aagctcttca agaggctgtt attacagaat tgataagtac aaatgtagca gataaagtaa
      481 gaaagccaaa aaagaaacaa tttgtttctc aagtttataa tcaacaagag atcttgaaat
      541 tacttgacat tttgtcagaa gaaaaactct atttggtagt tcttttaaca gcattttatg
      601 gattgcgaag aagtgaagtt ttaggattaa aatggtctgc tattgatttt ttaaacaaaa
      661 aaatcatgat aaaccatgta atcatagaaa atccagagaa tatgtcacag ttaataaaga
      721 aagatcaaac aaagaactct tccagctatc gtacattacc tttggttgaa acgattgaac
      781 gagcattagt aaatcaagct aaatggcaac aagataatcg tattctttta ggagatgatt
      841 atattttaga agataatgag tacgtattta ctatggaaga tggacggttg atgaagcctc
      901 aatatgtaac gcatcgtttg agtaagttga ttaagaaaaa tggattgaaa aaaattcgct
      961 ttcatgactt gcgtcattct aatgcttcaa taatgttaga tagtggccag aatatgaaat
     1021 ccattcaaga atggctggga catgcatcgt attcgacaac tgctaattta tacacgcatt
     1081 taaccgcagg agtaaaagat cgtgcagcag aagcattaga aaacgcattt gggtttaaag
     1141 aataaaaaaa caaaaaacaa tatctttggg gtatcgcttc ttgtttttag aatttttttg
     1201 ttggcaattt gttggcagta tgttggcaat gaaaaaattc acaaactaaa aacctattta
     1261 taaagcttta taaaactttt ttcacaaata gagcatttgg ttaagagtca gctgctctac
     1321 caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa gaattttaaa
     1381 atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag gaaatcattc
     1441 atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa gttcttgcaa
     1501 gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct ccccggcggg
     1561 tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt tgttttcatg
     1621 gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta tcataagtct
     1681 gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat
     1741 taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa gaactccctg
     1801 acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt tcacctgtga
     1861 tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac gttaaactag
     1921 gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca tttttacttg
     1981 caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg atattgttcc
     2041 aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa caagaagcag
     2101 ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg
     2161 caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca atgaaacttc
     2221 ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa
     2281 aacaataaaa tcgcaatttt agtagattca ggtacggatg ttccagaaga attgataaaa
     2341 aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt atatacagat
     2401 aaaattgaca ttacccctga agagatctat caacgtttgc cagaggaaat ccctggtaca
     2461 tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa agacggttat
     2521 gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa tgtggttcgt
     2581 ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa aaatattggt
     2641 attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg agtagaatgg
     2701 gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt
     2761 gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc tattgtagga
     2821 accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta ttacacagta
     2881 ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa
     2941 atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa agaagaagca
     3001 attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat ctactttggc
     3061 cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt cggtgtacaa
     3121 gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt
     3181 ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta attttctctt
     3241 ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga aggtatgaca
     3301 ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga tgagaagccg
     3361 atttcggata tcgtcaaatt caatttagcc aaagaaggct atgacgttta tactgcatat
     3421 gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat tttacttgat
     3481 ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa aacttatgat
     3541 atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct aggcttagag
     3601 ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt tgcacgtgtc
     3661 aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc agcacctgcg
     3721 gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac gaaacgagga
     3781 gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc gaagcacctt
     3841 ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga ttattttgga
     3901 gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga agataatcca
     3961 agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg taacccagaa
     4021 caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc
     4081 tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct actttatacg
     4141 tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa cggtggaatc
     4201 attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg atgaagaggt
     4261 aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca tcgaaatacg
     4321 ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt
     4381 gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt atgttgcttt
     4441 agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga
     4501 tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa
     4561 taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt caatgatcgt
     4621 atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag aacaagccat
     4681 tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag atgaacttgg
     4741 acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc aagatacaat
     4801 ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg gcgtcattgc
     4861 gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct tgctagatat
     4921 caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg aagaagaata
     4981 tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt caaccagtac
     5041 attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc gcgaatcagg
     5101 ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg
     5161 ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat
     5221 gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa
     5281 ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca atgacctctt
     5341 aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg tcaatttcaa
     5401 cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa
     5461 ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag atacagataa
     5521 aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac cagatggggg
     5581 aacgattact tgtcgcttat tagaaacgca taataacgtg attttaagta tcaccgatca
     5641 aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct atcgagtaga
     5701 taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt ctagagaagt
     5761 gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag ggtcgacttt
     5821 ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag aatctctgaa
     5881 aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt ctcttatgca
     5941 atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa cactcaaatg
     6001 atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca tgttacatgg
     6061 caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg
     6121 atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga caaatcacag
     6181 tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc gccttttttg
     6241 ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt ggcaaaagaa
     6301 agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa aatccgattt
     6361 ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg gacagatatc
     6421 aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga accatcgacg
     6481 atcgacacgc aatatgctac gaaacattcg atcaaactga agaagtatag ttatatcctg
     6541 tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa
     6601 aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat gtctatccat
     6661 tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga aaatgctagt
     6721 gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg gaacctcaga
     6781 tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg cttcccggtt
     6841 tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac gaatggttct
     6901 gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc ttcagacgaa
     6961 gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa gggagcagat
     7021 agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa agaaacgaat
     7081 cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg gtattcagct
     7141 aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt caaaaaaatc
     7201 gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag cagttatcat
     7261 gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc aatccaacaa
     7321 cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa caagcaaggc
     7381 tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta tcgcaaggct
     7441 ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt gacgaaatct
     7501 ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag tagttccttg
     7561 gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt gtacacgaaa
     7621 aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga gtatgaaggc
     7681 atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa cgataatgat
     7741 tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct gcgcgaaaag
     7801 acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa caaaatacca
     7861 agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa agtccgggaa
     7921 aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa agtggtccaa
     7981 attgagagcg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt accaaaagtg
     8041 gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa gggaagtagg
     8101 agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag tggaagttcg
     8161 ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc
     8221 ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca attagatgga
     8281 atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt ggctcgtaag
     8341 tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc actgattgga
     8401 aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac attcggcgat
     8461 atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc
     8521 cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag tgattatatg
     8581 cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga tcttgagatg
     8641 ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga tagaggacat
     8701 ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg gaccaaacga
     8761 atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca tttaacaatg
     8821 gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat ctatgataca
     8881 gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat aaaaatataa
     8941 aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt tttttagcat
     9001 attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt catgtttctc
     9061 atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct
     9121 agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac gtttgccatg
     9181 tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag attgtttcaa
     9241 ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat ttttaggtac
     9301 ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg cacccccaac
     9361 gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca aagaagtttg
     9421 atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat caaaagatgc
     9481 aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat ctgatggcaa
     9541 tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa
     9601 aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa aaaacctcct
     9661 tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc cgatttttga
     9721 aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta aattaagaaa
     9781 actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt tagatcgtgc
     9841 ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg
     9901 aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat ttgctcattt
     9961 tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt attttacaca
    10021 agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca ttgcatacgc
    10081 ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct tgtctgctta
    10141 tctactgctt attcagccac taaatactca gatagatact gtctggacag gtgctgaagg
    10201 gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac gtttagtaag
    10261 acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc agtcttttac
    10321 ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag cggtttttca
    10381 acatttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc ttcccattca
    10441 atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc cgtttttatg
    10501 gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg tcttgcaagc
    10561 gaattcatta gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg
    10621 cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg gtgctggatt
    10681 gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt
    10741 aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta ttttttccac
    10801 accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg
    10861 attgattacc tattccgctt tatattttgg attcgttcct ttatttacag cagtccaagt
    10921 cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc ctgcagcgat
    10981 tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt tgaaaaaaat
    11041 agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag cagaaaagat
    11101 gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca ccaatctagg
    11161 atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct aattgaacaa
    11221 gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc cgaccagttg
    11281 ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga tgaccatcat
    11341 tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa tttgaatatc
    11401 gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg ggtccagcaa
    11461 cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata ttttgtgtat
    11521 tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg atgctttgca
    11581 gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga gcagaagctt
    11641 tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa atagctagga
    11701 caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac gtagatttta
    11761 caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag caaaaaatgc
    11821 tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat aacaagcatg
    11881 caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag cagctgtcag
    11941 aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat gagtattatt
    12001 aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa agagttatcg
    12061 gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa
    12121 gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa accccaaact
    12181 gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc
    12241 gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga agtaaaatac
    12301 gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt tgagtaaatt
    12361 tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg gcaaaagaat
    12421 tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa ttagcagata
    12481 cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct
    12541 ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt
    12601 ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat gatattgcag
    12661 gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa
    12721 acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag
    12781 cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa gccattgcac
    12841 aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct gatgcaatgg
    12901 aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac
    12961 tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat atggtaacag
    13021 ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca gacaaaaaaa
    13081 tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa tcacgtccat
    13141 tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta ttgaacaaaa
    13201 tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag
    13261 caatgcttga agatatcgct attttgacag gtggtacagt aattactgac gatctaggtt
    13321 tagaattgaa agatgcaaca atcgaaaact taggaaatgc ttctaaagtt gttgtagata
    13381 aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc
    13441 aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa aaattacaag
    13501 aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca gctactgaaa
    13561 cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca cgtgccgcag
    13621 tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc agcaaagttt
    13681 ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag
    13741 aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg attgttgata
    13801 aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa tgggtaaaca
    13861 tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg caaaatgccg
    13921 cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa ccagaaccag
    13981 cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg tagtaattaa
    14041 gaaagaaccc tattttaaca gggttctttc ttcttttttt gttttatttt aagcaaaagg
    14101 ggcaaagaag gggcaaaaat atcaaaggct atttagttta tcaattactt gttgtttggc
    14161 tctttttgtt acatggttat aaatagacag agttgtgttt gcatcagaat gccctacacg
    14221 ctccataatg gctttaagag gtacgcctaa ttcagataat agtgaaacgt ggctatgtct
    14281 gaatatatgt gaggatagac ttttttctaa ttccaactct tcctctactt tatggagtat
    14341 agcgttaaat gagtgcagcg caagcggagt gccacttgta gatataaata tatattgatc
    14401 gggatctgtg ggtctacctg caagaatatt gtcagctatc acactttcaa tcaattcttt
    14461 tgcacgattg ggtaattgca cttcacgttg cgaataagta ttttttggag ttgtttttat
    14521 agcattatcc attttcacag atgtgtaatc taaggtccca ttaatggaaa ttttcccatc
    14581 ctcatagtcc ttcatttgca aagctagcaa ttccccatat ctcaaaccag ttaaatatag
    14641 aaactcagct attatgccgt gtagttttct gcgaggattg gagtatagct gtttcagtat
    14701 ttgatcaatt tcttctttct ccagatattt tttattcata gaaagccttc ttttttcttc
    14761 ttccactttt ttaggatgga ttttaactgc tagcgcaggg tttctttgaa tgtattttct
    14821 atcgattgca tagtttagca taacagatag agttgttttt gtttgttttg tgtagttcag
    14881 tgagaggtca ccaaacgtat acatatcttc aattatctta ttaatgagtg tctcatcaat
    14941 gtttctaacg atcgtatcat cgcttatgtg cttagaaaca tgtttcatca tcattggaac
    15001 cttcaaatag ctagtacgtt taacatgctg cttataatat tcataccatt ctttatacag
    15061 ctcaccaaaa gtgatatctg atttattgta atcttcgagt gcttctttaa tttttttatc
    15121 tagaattttc tgagcttttt tccacgcttg tggtgaatta cttgtaagtg ttgtagattt
    15181 ttttcgtgtt ttttctgtat aaggatctac atatctttca ataaacttga attggccatc
    15241 tttggtctgt tcaacccaca cttttaacat ctcctatcat ttgctataat aggcataaca
    15301 aatagaccta tataggtttg ttttctaaaa gcacgctctt actctggacg gt
//
LOCUS       DACDCV010000068        14245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDCV010000068 DACDCV010000000
VERSION     DACDCV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.068"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(197..445)
                     /locus_tag="IV041_002020"
     CDS             complement(197..445)
                     /locus_tag="IV041_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382780.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            444..572
                     /locus_tag="IV041_002021"
     CDS             444..572
                     /locus_tag="IV041_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8382781.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            629..2323
                     /locus_tag="IV041_002022"
     CDS             629..2323
                     /locus_tag="IV041_002022"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8382782.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            2423..2596
                     /locus_tag="IV041_002023"
     CDS             2423..2596
                     /locus_tag="IV041_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382783.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            2974..4107
                     /locus_tag="IV041_002024"
     CDS             2974..4107
                     /locus_tag="IV041_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8382784.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            4138..4458
                     /locus_tag="IV041_002025"
     CDS             4138..4458
                     /locus_tag="IV041_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382785.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(4487..4951)
                     /locus_tag="IV041_002026"
     CDS             complement(4487..4951)
                     /locus_tag="IV041_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382786.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(5083..6336)
                     /locus_tag="IV041_002027"
     CDS             complement(5083..6336)
                     /locus_tag="IV041_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382787.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(6365..6682)
                     /locus_tag="IV041_002028"
     CDS             complement(6365..6682)
                     /locus_tag="IV041_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8382788.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(6708..7028)
                     /locus_tag="IV041_002029"
     CDS             complement(6708..7028)
                     /locus_tag="IV041_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8382789.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(7492..8907)
                     /locus_tag="IV041_002030"
     CDS             complement(7492..8907)
                     /locus_tag="IV041_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8382790.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(8924..10240)
                     /locus_tag="IV041_002031"
     CDS             complement(8924..10240)
                     /locus_tag="IV041_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382791.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(10390..11088)
                     /locus_tag="IV041_002032"
     CDS             complement(10390..11088)
                     /locus_tag="IV041_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8382792.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(11377..12366)
                     /locus_tag="IV041_002033"
     CDS             complement(11377..12366)
                     /locus_tag="IV041_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8382793.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(12446..13279)
                     /gene="msrA"
                     /locus_tag="IV041_002034"
     CDS             complement(12446..13279)
                     /gene="msrA"
                     /locus_tag="IV041_002034"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8382794.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(13454..>14245)
                     /locus_tag="IV041_002035"
     CDS             complement(13454..>14245)
                     /locus_tag="IV041_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382795.1"
                     /translation="SMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPW
                     YLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITL
                     TVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEA
                     KIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGL
                     VQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         4402 a         2885 c         2424 g         4534 t
ORIGIN      
        1 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
       61 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
      121 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
      181 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
      241 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
      301 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
      361 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
      421 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
      481 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
      541 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
      601 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
      661 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
      721 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
      781 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
      841 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
      901 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
      961 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     1021 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     1081 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     1141 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     1201 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     1261 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     1321 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     1381 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     1441 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     1501 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     1561 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     1621 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     1681 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     1741 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     1801 tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     1861 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     1921 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     1981 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     2041 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     2101 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     2161 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     2221 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     2281 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     2341 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     2401 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     2461 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     2521 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     2581 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     2641 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     2701 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
     2761 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
     2821 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     2881 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     2941 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     3001 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     3061 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     3121 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     3181 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     3241 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     3301 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     3361 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     3421 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     3481 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     3541 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     3601 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     3661 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     3721 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     3781 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     3841 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     3901 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     3961 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     4021 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     4081 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     4141 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     4201 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     4261 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     4321 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     4381 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     4441 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     4501 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     4561 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     4621 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     4681 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     4741 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     4801 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     4861 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     4921 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     4981 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     5041 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     5101 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     5161 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     5221 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     5281 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     5341 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc
     5401 tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata
     5461 aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa
     5521 gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat taacgtttgc
     5581 ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa
     5641 accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt
     5701 tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac
     5761 accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg
     5821 tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat
     5881 caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat
     5941 agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc
     6001 taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc
     6061 aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc
     6121 gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat
     6181 cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat
     6241 aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat
     6301 aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt ttctttactg
     6361 tagattacgc gttcaatcga cgatacagat taatgatctc ttttgctaaa tctgtaaaag
     6421 ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat
     6481 ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt
     6541 cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa
     6601 tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt
     6661 ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt
     6721 agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg
     6781 attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag
     6841 cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca
     6901 tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc
     6961 atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc
     7021 ttttccattt taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc
     7081 caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc
     7141 atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt
     7201 cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa
     7261 cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt
     7321 tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat
     7381 aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct
     7441 tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata
     7501 gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga
     7561 ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt
     7621 cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca
     7681 atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat
     7741 acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc
     7801 cataccgttt tctgttatca gccaagggat attatgataa ttttctttta gatacatggc
     7861 tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc
     7921 aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg
     7981 ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt
     8041 atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg
     8101 acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg
     8161 attataaaac aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc
     8221 atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta
     8281 aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt
     8341 tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat
     8401 tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg
     8461 agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa
     8521 aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat
     8581 caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc
     8641 ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga
     8701 tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc
     8761 atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg
     8821 tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat
     8881 aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt
     8941 ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa
     9001 caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg
     9061 gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa
     9121 aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat
     9181 tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa
     9241 gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca
     9301 gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag
     9361 taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg
     9421 ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc
     9481 aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt
     9541 ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt
     9601 tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag
     9661 aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct
     9721 gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata
     9781 accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt
     9841 cattcactaa ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac
     9901 cagggaatac accatccact ttattcagct tggctaattc gatccctatc agaaaaacag
     9961 catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt
    10021 cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg
    10081 ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt
    10141 tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat
    10201 tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac
    10261 gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc
    10321 ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca
    10381 gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat
    10441 tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg
    10501 aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt
    10561 attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc
    10621 gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc
    10681 ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt
    10741 cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa
    10801 taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc
    10861 aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct
    10921 ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga
    10981 caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct
    11041 aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc
    11101 cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt
    11161 tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat
    11221 aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt gaacgacaaa
    11281 aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt
    11341 tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct
    11401 caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg
    11461 acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca aattccatgc
    11521 aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat
    11581 tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc
    11641 tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg
    11701 actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag
    11761 tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct
    11821 ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata taccaatata
    11881 taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt ccaaaggtgt
    11941 atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca ttctttggag
    12001 actttggtca caacttgaaa tgatggattt acgagagccc cattccccat atggcccttc
    12061 ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg cttgaaaatc
    12121 ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc cataattatt
    12181 cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt
    12241 atggatagga tcataatcac caaaattttg ccataatccg agagataaaa tcggtaattt
    12301 caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat
    12361 atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt ctatttttag
    12421 tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata tttttttgcc
    12481 atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg atttttttat
    12541 aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc caaaacgtac
    12601 cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg tctagtttct
    12661 gttttgattg ttccgctaat tctttttgtc gttcgacttg ataaaaaatc actggacgat
    12721 attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta atttgccaat
    12781 agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc tccacagctt
    12841 ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca acatgtccgc
    12901 ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc tcgaccatac
    12961 accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct tgcatggcat
    13021 gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat aaataaaagt
    13081 ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt gcttctagtt
    13141 ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc tgaatcaata
    13201 cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt gtttgaatga
    13261 atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct agtgacttta
    13321 gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta cagatttttc
    13381 gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt tattttataa
    13441 atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat aaatatgcag
    13501 ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt actttaatat
    13561 cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta
    13621 caggttgatg atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta
    13681 cttggctctt ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa
    13741 ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca
    13801 atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct
    13861 ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat
    13921 tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata
    13981 gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag
    14041 agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat
    14101 tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat
    14161 aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag
    14221 ataacagacc aactagtccc attga
//
LOCUS       DACDCV010000069        14237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDCV010000069 DACDCV010000000
VERSION     DACDCV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.069"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(149..3802)
                     /gene="rpoC"
                     /locus_tag="IV041_002036"
     CDS             complement(149..3802)
                     /gene="rpoC"
                     /locus_tag="IV041_002036"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8382796.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3881..7498)
                     /gene="rpoB"
                     /locus_tag="IV041_002037"
     CDS             complement(3881..7498)
                     /gene="rpoB"
                     /locus_tag="IV041_002037"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8382797.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8226..9194
                     /locus_tag="IV041_002038"
     CDS             8226..9194
                     /locus_tag="IV041_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8382798.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9212..10117
                     /locus_tag="IV041_002039"
     CDS             9212..10117
                     /locus_tag="IV041_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382799.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10114..10920
                     /locus_tag="IV041_002040"
     CDS             10114..10920
                     /locus_tag="IV041_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8382800.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11077..12045
                     /locus_tag="IV041_002041"
     CDS             11077..12045
                     /locus_tag="IV041_002041"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8382801.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12362..12886)
                     /locus_tag="IV041_002042"
     CDS             complement(12362..12886)
                     /locus_tag="IV041_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8382802.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13173..>14237
                     /gene="scm"
                     /locus_tag="IV041_002043"
     CDS             13173..>14237
                     /gene="scm"
                     /locus_tag="IV041_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8382803.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4035 a         3068 c         2806 g         4328 t
ORIGIN      
        1 ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc
       61 cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
      121 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
      181 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
      241 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
      301 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
      361 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
      421 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
      481 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
      541 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
      601 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
      661 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
      721 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
      781 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
      841 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
      901 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
      961 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
     1021 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
     1081 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
     1141 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
     1201 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
     1261 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
     1321 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
     1381 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
     1441 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
     1501 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
     1561 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
     1621 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
     1681 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
     1741 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
     1801 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
     1861 accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga
     1921 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
     1981 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
     2041 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
     2101 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
     2161 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
     2221 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
     2281 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa ggagtaacaa
     2341 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
     2401 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
     2461 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
     2521 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
     2581 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
     2641 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
     2701 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
     2761 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
     2821 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
     2881 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
     2941 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
     3001 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
     3061 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
     3121 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
     3181 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
     3241 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
     3301 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
     3361 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
     3421 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
     3481 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
     3541 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
     3601 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
     3661 agaacaaccc ttcacgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
     3721 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
     3781 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
     3841 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
     3901 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
     3961 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
     4021 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
     4081 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
     4141 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
     4201 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
     4261 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
     4321 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
     4381 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
     4441 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
     4501 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac
     4561 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
     4621 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
     4681 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
     4741 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
     4801 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
     4861 cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga
     4921 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
     4981 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
     5041 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
     5101 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
     5161 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
     5221 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
     5281 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
     5341 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
     5401 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac
     5461 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
     5521 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
     5581 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
     5641 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
     5701 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
     5761 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
     5821 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
     5881 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
     5941 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
     6001 gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag
     6061 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
     6121 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
     6181 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
     6241 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
     6301 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
     6361 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
     6421 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
     6481 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
     6541 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
     6601 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
     6661 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
     6721 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
     6781 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
     6841 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
     6901 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
     6961 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
     7021 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
     7081 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
     7141 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
     7201 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
     7261 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
     7321 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
     7381 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
     7441 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
     7501 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
     7561 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
     7621 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
     7681 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
     7741 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
     7801 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
     7861 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
     7921 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
     7981 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
     8041 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
     8101 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
     8161 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
     8221 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
     8281 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
     8341 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
     8401 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
     8461 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
     8521 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
     8581 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
     8641 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
     8701 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
     8761 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
     8821 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
     8881 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
     8941 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
     9001 gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca gggatcatgg
     9061 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
     9121 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
     9181 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
     9241 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
     9301 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
     9361 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
     9421 gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
     9481 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
     9541 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
     9601 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
     9661 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
     9721 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
     9781 ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg gctttgctga
     9841 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
     9901 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
     9961 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
    10021 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
    10081 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    10141 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    10201 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    10261 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    10321 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    10381 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    10441 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    10501 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    10561 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    10621 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    10681 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    10741 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    10801 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    10861 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    10921 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    10981 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    11041 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    11101 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    11161 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    11221 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    11281 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa
    11341 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    11401 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    11461 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    11521 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    11581 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    11641 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    11701 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    11761 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    11821 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    11881 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    11941 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    12001 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    12061 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    12121 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    12181 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    12241 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    12301 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    12361 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    12421 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    12481 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    12541 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    12601 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    12661 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    12721 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    12781 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    12841 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    12901 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    12961 cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa
    13021 gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct
    13081 ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata
    13141 ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc
    13201 tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca
    13261 ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa
    13321 ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg
    13381 gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag
    13441 gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag
    13501 gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag
    13561 cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg
    13621 atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat
    13681 tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag
    13741 attttccaga catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt
    13801 tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc
    13861 caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt
    13921 caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata
    13981 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    14041 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    14101 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    14161 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    14221 atagctcaga ttcatca
//
LOCUS       DACDCV010000070        14106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDCV010000070 DACDCV010000000
VERSION     DACDCV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.070"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..766
                     /locus_tag="IV041_002044"
     CDS             65..766
                     /locus_tag="IV041_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8382804.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="IV041_002045"
     CDS             763..1884
                     /locus_tag="IV041_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8382805.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="IV041_002046"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="IV041_002046"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8382806.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="IV041_002047"
     CDS             3263..4171
                     /locus_tag="IV041_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8382807.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="IV041_002048"
     CDS             4206..4478
                     /locus_tag="IV041_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8382808.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="IV041_002049"
     CDS             4489..5535
                     /locus_tag="IV041_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8382809.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5786..8395
                     /gene="clpB"
                     /locus_tag="IV041_002050"
     CDS             5786..8395
                     /gene="clpB"
                     /locus_tag="IV041_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8382810.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8546..9217)
                     /locus_tag="IV041_002051"
     CDS             complement(8546..9217)
                     /locus_tag="IV041_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382811.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9210..9704)
                     /locus_tag="IV041_002052"
     CDS             complement(9210..9704)
                     /locus_tag="IV041_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8382812.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9724..10944)
                     /locus_tag="IV041_002053"
     CDS             complement(9724..10944)
                     /locus_tag="IV041_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382813.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11091..12926
                     /gene="lepA"
                     /locus_tag="IV041_002054"
     CDS             11091..12926
                     /gene="lepA"
                     /locus_tag="IV041_002054"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8382814.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13020..14102)
                     /locus_tag="IV041_002055"
     CDS             complement(13020..14102)
                     /locus_tag="IV041_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382815.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4040 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac aatcaatttt
     5641 gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg tatattttga
     5701 ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata agtgatctta
     5761 cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga
     5821 agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc
     5881 ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct atacggatgc
     5941 cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt
     6001 tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt
     6061 gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt
     6121 tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg
     6181 attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac
     6241 ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca
     6301 gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt
     6361 catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt
     6421 agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga
     6481 aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa
     6541 attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga
     6601 tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga
     6661 aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt
     6721 gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga
     6781 acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct
     6841 gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt
     6901 agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc
     6961 aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac
     7021 ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa
     7081 aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga
     7141 acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa
     7201 ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga
     7261 aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga
     7321 aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga
     7381 gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac
     7441 gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg
     7501 agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc
     7561 tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt
     7621 cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat
     7681 ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc
     7741 tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa
     7801 tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat
     7861 cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt
     7921 caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt
     7981 gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg
     8041 aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag
     8101 cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga
     8161 acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc
     8221 ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac
     8281 accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac
     8341 tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc
     8401 cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc
     8461 aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg
     8521 cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt
     8581 caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac
     8641 ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca
     8701 gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga
     8761 attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga
     8821 acaaccaagg actggttttt gatatctcag catctttcat caagtaacca atcaatccat
     8881 agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga
     8941 tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt
     9001 aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca
     9061 tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg
     9121 aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa
     9181 gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat
     9241 ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc
     9301 aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca
     9361 gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact
     9421 gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc
     9481 ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca
     9541 atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga
     9601 tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac
     9661 aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt
     9721 ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt
     9781 cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata
     9841 attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc
     9901 atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca
     9961 aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa
    10021 ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc
    10081 tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg
    10141 tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc
    10201 ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc aatagaacag
    10261 aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt
    10321 ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg
    10381 ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac
    10441 cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt
    10501 atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata
    10561 agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat
    10621 caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata
    10681 acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt
    10741 ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa
    10801 agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata
    10861 ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt
    10921 ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga taacagtata
    10981 gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc
    11041 ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa
    11101 agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc
    11161 atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg
    11221 aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta
    11281 aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact
    11341 tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct
    11401 gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca
    11461 atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc
    11521 tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg
    11581 ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc
    11641 agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga
    11701 tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg
    11761 gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta
    11821 cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg
    11881 tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa
    11941 cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg
    12001 tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag
    12061 aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag
    12121 gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct
    12181 tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta
    12241 ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa
    12301 cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg
    12361 tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt
    12421 tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact
    12481 ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg
    12541 gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg
    12601 cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa
    12661 aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac
    12721 aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt
    12781 atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc
    12841 ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa
    12901 tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa
    12961 ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg tggcaaaaat
    13021 caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat cttatctgtt
    13081 acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg ttcagagatt
    13141 tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct gagagaatgg
    13201 aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt cactacactt
    13261 ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat gtagagcttt
    13321 ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat tgctagcaag
    13381 gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc atatacccat
    13441 gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga aaaatcgaca
    13501 tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa tccaatattc
    13561 aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc aaagtcctca
    13621 agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt tggtattttt
    13681 acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat attcagcgca
    13741 ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt tttaaattca
    13801 gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt ctttcctaaa
    13861 taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt gtattttaga
    13921 tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg agtatccgta
    13981 cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa agcagcttcg
    14041 gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc atcatcattg
    14101 atatct
//
LOCUS       DACDCV010000071        13973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDCV010000071 DACDCV010000000
VERSION     DACDCV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.071"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IV041_002056"
     CDS             950..1423
                     /locus_tag="IV041_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8382816.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IV041_002057"
     CDS             1432..1659
                     /locus_tag="IV041_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382817.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="IV041_002058"
     CDS             2295..2666
                     /locus_tag="IV041_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382818.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="IV041_002059"
     CDS             complement(2922..3164)
                     /locus_tag="IV041_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8382819.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="IV041_002060"
     CDS             complement(3196..4098)
                     /locus_tag="IV041_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8382820.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="IV041_002061"
     CDS             complement(4111..4299)
                     /locus_tag="IV041_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8382821.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IV041_002062"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IV041_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8382822.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="IV041_002063"
     CDS             complement(4914..5807)
                     /locus_tag="IV041_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8382823.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6823)
                     /locus_tag="IV041_002064"
     CDS             complement(5885..6823)
                     /locus_tag="IV041_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8382824.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="IV041_002065"
     CDS             complement(6857..7207)
                     /locus_tag="IV041_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8382825.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="IV041_002066"
     CDS             complement(7240..8142)
                     /locus_tag="IV041_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8382826.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="IV041_002067"
     CDS             complement(8135..8992)
                     /locus_tag="IV041_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8382827.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="IV041_002068"
     CDS             9326..10135
                     /locus_tag="IV041_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8382828.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="IV041_002069"
     CDS             10175..10672
                     /locus_tag="IV041_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8382829.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="IV041_002070"
     tRNA            complement(11057..11130)
                     /locus_tag="IV041_002070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="IV041_002071"
     CDS             11317..11640
                     /locus_tag="IV041_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382830.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="IV041_002072"
     CDS             11804..12058
                     /locus_tag="IV041_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382831.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="IV041_002073"
     CDS             12128..12373
                     /locus_tag="IV041_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8382832.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="IV041_002074"
     CDS             12449..12814
                     /locus_tag="IV041_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382833.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="IV041_002075"
     CDS             complement(12875..13438)
                     /locus_tag="IV041_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382834.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4098 a         2582 c         2306 g         4987 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc aactttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatgaatttt ttcgtacaaa aaaaccgtct ttt
//
LOCUS       DACDCV010000072        13938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDCV010000072 DACDCV010000000
VERSION     DACDCV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.072"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            293..1930
                     /locus_tag="IV041_002076"
     CDS             293..1930
                     /locus_tag="IV041_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8382835.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2075..2815)
                     /gene="truA"
                     /locus_tag="IV041_002077"
     CDS             complement(2075..2815)
                     /gene="truA"
                     /locus_tag="IV041_002077"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8382836.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3006..3641)
                     /locus_tag="IV041_002078"
     CDS             complement(3006..3641)
                     /locus_tag="IV041_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8382837.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3788..5029)
                     /gene="sstT"
                     /locus_tag="IV041_002079"
     CDS             complement(3788..5029)
                     /gene="sstT"
                     /locus_tag="IV041_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8382838.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5227..6225
                     /locus_tag="IV041_002080"
     CDS             5227..6225
                     /locus_tag="IV041_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382839.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6238..8256
                     /locus_tag="IV041_002081"
     CDS             6238..8256
                     /locus_tag="IV041_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8382840.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8316..9032)
                     /locus_tag="IV041_002082"
     CDS             complement(8316..9032)
                     /locus_tag="IV041_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8382841.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9259..9801)
                     /locus_tag="IV041_002083"
     CDS             complement(9259..9801)
                     /locus_tag="IV041_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8382842.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9806..10033)
                     /locus_tag="IV041_002084"
     CDS             complement(9806..10033)
                     /locus_tag="IV041_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8382843.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10112..11992)
                     /locus_tag="IV041_002085"
     CDS             complement(10112..11992)
                     /locus_tag="IV041_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8382844.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12181..12795)
                     /locus_tag="IV041_002086"
     CDS             complement(12181..12795)
                     /locus_tag="IV041_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8382845.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12937..13497
                     /locus_tag="IV041_002087"
     CDS             12937..13497
                     /locus_tag="IV041_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8382846.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13802..>13938)
                     /locus_tag="IV041_002088"
     CDS             complement(13802..>13938)
                     /locus_tag="IV041_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8382847.1"
                     /translation="NSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR
                     "
BASE COUNT         4362 a         2689 c         2191 g         4696 t
ORIGIN      
        1 agaagataac cctttgattc gtgaaagatg aaaagataaa tcacctgttg tataaatttc
       61 ttgagcattt ctcataaaat ttactgaata taaagtacta agtaagataa gaactactaa
      121 aagaaatgct tttttactta ttttcataga tttgttgttc tcctttttta attctatttt
      181 cagtatacta gaattgtctt tttttaataa gttttgcgtt aaatttttca tagcactagt
      241 atgatatact agaaaagaat caatttttat tacttttaaa taggagaaaa aaatgaaaaa
      301 aaacaaaaac caaataatag atatcatttt tatgttattt ctaagttttg cttacgtcat
      361 ccctctattc atgtctaagg gaatctatca tagtgtcaat caggatactt actttcattt
      421 atctcgaatc ataggactag ataatgtatg gagttctcca gtaaacttca ataacttcga
      481 tcatcatgga acaatgatga atatttttta tccatggctt actctttatc cagctttttt
      541 attttataaa atgatgggaa atcttgtttt aagttataat atctactatt tttttatcac
      601 ttttttaaca atggtcgttt cttatttttc tatgaaacaa ataaaaaaca atcgttatat
      661 ttcgctgtta ttttcgatta tttatacatt ttccgcctat cgtgccattg acatattcca
      721 tcgtgcttca ttgggagaag ctgtcgcttt aacatttttg cctttgatac taatggggtg
      781 ttacgaaatt tatattagag attaccaaaa atggtactgg ttgtctattg gcatgacgct
      841 agtagtgtat actcatttac tttctgtcgc tatggtttct gtatttattg gaggaacgtt
      901 gtttctttct ttttattttt gggaccaaaa aatcgctaga ctcttaagtc ttttaaaagc
      961 taccgtcctc accttttttt taagtgcggg attcttaatt ccttttattc aacaatctag
     1021 agcacaagaa ttgaaagtcc cactgggaaa agaattaagt ggtatggctc ctagtgatat
     1081 gctgacacat attttaaata ataactataa caactatact attggattat tcttatttct
     1141 cggattgatt ggtgctttta tttttataaa aaaattaact gctgatgatt tatttatttt
     1201 ctttcttggt gtatttgttc ttttttgttc aaccaattta tttccttggc aactttttaa
     1261 ccatacacca gttaagagtc tacaatttgt ctggcggtta aatggttttt cttctttatt
     1321 tatagcctat acgatgagta tagtgatcta ttatattttt tctactaaaa ataattcatg
     1381 gaaaataatg gtctttacgt ttcttgcact aatattgcat ctctcaggag taagcaattc
     1441 tatccatgct aacaaagatt cattaacact tatagcacct gaagacgctg ttgctatagc
     1501 tgaaaattac aatcatactg attatgcaaa caaagagtca atctatcatc ctgatatgat
     1561 taataatgat caatatttac taggtaacca ggaaataaat ccaacaactc attttatgag
     1621 taacaaactt acaattgaat tagataattc gaataataaa aatactgtct tgacgacacc
     1681 tatttatcgt tataaagggc aagtggcttc tattaatggc aaactagtac aaacaaaatt
     1741 atctaagttt ggcacaactg aattaactat tcctccaggt ataaataaag tcgttattac
     1801 ttatcagtac acaaaactag caattgcttc aagatattta tctatcgtta ctttgatttt
     1861 atttttactt tatcgattta ctttttctaa acaaaaacaa ccgagaagag aagtacaaca
     1921 ttctcactag atttttattt aagaaagact ttttagctgt acacataaac aggctaaaaa
     1981 gtcttttttt atatttcttt aatttgcttc atacattatt taacatcgat atttatactg
     2041 tgaaacatgc gtttcatcca ttatcaaagt atttttatcg ataataaact tcatccaaaa
     2101 acagaccttg tgcagggacg gtttctcctg catgttgccg cactttgctt tcaaatattt
     2161 catcgattgt ttctatcggc atggcacctg tgccgatttc tagtaatgtc cccatgataa
     2221 tccgcaccat tttatataag aatccgtctc cgacaaaagt aaaatgaagc atttctccct
     2281 ttcgttcgat agataattca tcaatcgtac gcactgtgga tttttttgat tttttcaatg
     2341 cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc gcaagctgca ttcagcttgt
     2401 ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct gaaggaatca
     2461 cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt gaatggaagc
     2521 gctcaggcat ttcttcgatt cttttgatga caatatcccg cggaaggtaa cgattcataa
     2581 agtcgatcat ttctgccagt ccaatttctg tgtttgtttt aaaatttgct acttgtcctc
     2641 gcgcatgggt gccagcatct gttcttcctg atccaataat ttctatctct tcatttgtca
     2701 tctgatgcaa tactttttcg atttttcctt gaatggtttt atcgtcatct ccaagtcttt
     2761 gccatcctga atatcttttt ccgtcatatt cgattgtcaa cttgatgttt ctcatatttt
     2821 agtggctcct ctgtcttcca tttggtcttc tctctacctt tttaagattg tagcacaaaa
     2881 tatagctcct tgcttctcta agacagaagc tatacaagaa aactgtggaa atatatattt
     2941 cagaaaaaca aaaaacagcc accggatgaa gtatccgctg actgttcaat ttgacggctc
     3001 aagttttact gtttgagatg caagatactc aacctggcaa tgggcacata attaaagaaa
     3061 aaactgattc tttggcaaaa acctatttta ttcagtaatg gccaagaatt tatagaagga
     3121 attcttgcca gtccataaat cgccgcaaaa agcaaactga tcacagacag tccaaggtat
     3181 aagtggctta cgtccatttg cccatttttt cgatagatca agaaaagaag aactggaaac
     3241 aataaaaaac cactgtaacc taaacctgat ccgatattat ggacccatgt cgagacattc
     3301 cacgaagatt catgggtatt gatactgaac aaacctgtaa agatacaatc acctatacca
     3361 tagaacgcga tggctgctgt aactagggta gctaacacac gagatgtact aacaaatgca
     3421 gcatacaatg caggcaatga caggatgaaa aagatccctg aaaccacaga ccaaataaga
     3481 aacgcttttc ttacaggact ggaaacctct ccaaatacac tgatcacgct cttcatttga
     3541 tctaattcag gataatacaa tcctaatacg tacggagtca aaaaatcact cagcacgcct
     3601 aataataaaa agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct ccaataacaa
     3661 ttacctatgc ttttaattgt tattataaac gactttatct tgatttaaca gaaacctcta
     3721 tttacacgaa catctatata actcattaaa aactcgaatc attttaaaaa tactaaatat
     3781 ctaacgttca acgttcactt aatccatcta aacaatcttt caatttgatt ttttccccac
     3841 ttcttttacg cgcgcctagc tctcctattg cagtaaacaa cagatcactt gacgaattta
     3901 aggcagtttc cattgaatcc tggataacac cgataataaa tccaataccg acgatttgca
     3961 tcgcgacatc attagagata ccaaacaaac tacatgctaa tggaatcaat aacagtgaac
     4021 cgcccgcaac acctgaagct ccacaagcag ctaaagcaga caaaatacat aaaaggactt
     4081 tcaaaacaaa aggtgcttca atccctaaag aatgtacagc agtcaacgtc atcaaggaaa
     4141 tggtaatcgc ggcaccgccc atattgatcg tagcacctaa cgggatcgat atggaataag
     4201 actcttttgt caatttcaat ctttcagcca acatcatgtt tatcgggata tttgcggcag
     4261 aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa atcaatggat
     4321 agggattttc tttaataaac aaaaacgtga ggaatgggta cacaactaat gcgacaaaca
     4381 gcatcgtacc gatcaaaaca agtaaaagct gcagatactc tgccaatcca gcgattccag
     4441 tcgtagcaat tgattgataa accaatccta aaatccctat cggagcaaat gcaataatca
     4501 cttgtacgac ttgtgacaac gctatagata tctgatccac caactctttt gtcgattctg
     4561 aacgtgctcg aaaagctagt cccaacaata cagcccagaa taagacgcca aggtaattcg
     4621 catcaataag agattgaaca ggattttgtg tgacattcgt caacatagta gataaaacac
     4681 tttccaaact ttgcggtgct ttttcagata ctgtttcttg taaagtaata ggaacagtaa
     4741 aaattgaact tacaataact gctacaagtg cagaaagcaa tgttcccact aaatataaga
     4801 tcaaaatagg cttcacgaat gtcttattcc caattttatg cttggcaatt gacgccatcg
     4861 tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca actgacaaac aacgttccca
     4921 atattgaaat aaatgtccat gtggggaaaa agataccaaa caatattccg actaaaatac
     4981 caacaacaat acgttgaact aacgacatat ccaaaaactt ctctaacaaa tgaccaccct
     5041 ttcacaattt taacaatcat tgtatcattt tatgcaaaga aactaaaggt ataattttct
     5101 aaaaattcta acaatacaaa caaccatttc ttgctaattt tgtacttttt ctgtctacac
     5161 gttgtttttt gagatattct aagttaggat gttattaatg ggcaacttat caggaaacgt
     5221 tttcttatgt ttttgtccaa tttttttgtg aggtgtttat tcttgaaaaa aataaagaag
     5281 gagagttatg gcttcctgac aatctttgtc gcttgtataa tgatggtttt acagcgtata
     5341 ttgatttcag cagtcactat tcctacatat gcaacaaacc ctggtcgatt ctacatctat
     5401 acatttttac aaacttccgt tgttattggt gcaaatttga tcccacttta catgggttac
     5461 caatatcaaa aaatcgcccg tttaaaagtt ttacattatt taagtcgttt tgcctttata
     5521 ttcctaatag ccacactagt ttgcaatatc tttttttacg tacaagctgg cagcttgaat
     5581 attcgtgatt attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt
     5641 tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg
     5701 aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact
     5761 ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat
     5821 ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag
     5881 cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa
     5941 atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct
     6001 ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa
     6061 ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata
     6121 tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac
     6181 tcagggaagg catggctgat aaatatcggg cagtttttta tttgatattt attagcagtg
     6241 tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc
     6301 gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat
     6361 tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc
     6421 gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta
     6481 aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt
     6541 ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc
     6601 attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc
     6661 tttccttcag atttaaaaat gctgaccgga ttgtcagaac tattatcgat ggttagccca
     6721 gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta
     6781 caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt
     6841 atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct
     6901 tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc
     6961 gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa
     7021 gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag
     7081 gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat
     7141 ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct
     7201 attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt
     7261 tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac
     7321 ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca
     7381 aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat
     7441 cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg
     7501 gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca
     7561 tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt catccaatta
     7621 tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct
     7681 gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt
     7741 cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg
     7801 attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa
     7861 aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt
     7921 gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct
     7981 cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat
     8041 catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa
     8101 caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa
     8161 aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac
     8221 agtgcaacat gggcgacaca aaaaataaaa gactaacaaa taaactgagc aaggggttgt
     8281 gataaaagac atgtcacaac cccttgttta tctcattaat ttgcttgtcc ataaacgatt
     8341 tgattaaagc catttttcaa ttcattattc gctgtctcgt tgaagtaaag ataattatcg
     8401 ggattcccct gattttgttg tgtcaccaat acttttggct gattttgctg aaacccgaat
     8461 agatagacgt gctttttcag atatggttct gtctctgcat cggaatagac ggctgtgatt
     8521 tgatagtctg cttgacctgt cccactttct gaccattctg cagaaacagg cactccacca
     8581 atagccatct tccactcccc gttcaaaata gcttgaggga ctttaagacc atataagtcc
     8641 acactcattt gattcgtata acttttgtat tgttgtccca ttgtttggcc ccatttggtc
     8701 ataaatgcct ctaattgtgc ggatttgtct gcattccaca gtgtttgttc aacttgttcg
     8761 ctgctttctg aagaggaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt
     8821 gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct
     8881 gcagttgact tgctctctgt gctagttgtt gtagtagttg caactgttgc tttcgctgtt
     8941 tcttttgttg attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct
     9001 agaccaattg ttactaaaat cctttttttc aattaaaaaa ctcctttcga ttactcccac
     9061 ttctttattc tatcaaataa gcaatgtgat tttattgatt tttcgtattt tctcatcaat
     9121 ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat acatcagcgg
     9181 gtgcttcttt tattttaatc ttcgtgattt tccaatttac ctcacgcttt atatttgtat
     9241 ttgaagaaac atagactttt agtcatcatc gtcttcctca tccaaatctt gtcggacagt
     9301 ttttccttgg agtctttgta cttggtagat gatttcttta ttgaaactta aaagttcaac
     9361 aagcaattgt tgcaagccat atttttcttc tttttcagct aatttgattg cccgtgtgat
     9421 aaagacattt tggaaatcaa aatcttttgc aaagttttcc aacattgttg aggctggtaa
     9481 atatttgttt tgaccatgtt cgtaaatttt tccataacgc atgacttctt ctaaagtaga
     9541 cggaacgatt tcattttcaa tcactaactc atgagagatt ttattgaacc atctcgcttc
     9601 ttgatcagca tattctgaaa aaagggtact taattcttgc gaagatgtac ccaaaacata
     9661 caattgtgct tggatcaatt tcatttcttc taatttaaaa tttgctaata catgccccac
     9721 catggcgcca gcagttggtt tatgatggtc gatatctgct tgttttgttt cttgaacaaa
     9781 tttttcttcc actatattca ttgatttacg cctcttttac ttttgctgat tggacttcat
     9841 aacctaattt ttccacaact gcagctaatt gttcagggtg gacttcatct ggatcaaagt
     9901 ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag
     9961 aaaccgcatg ttcgattttt tgcatacagc ttggacaagc tagtgtatct aattgcataa
    10021 taattttttt catcgtagtt cttccttcct ttttcttgat agtcttattc tagattacag
    10081 cttccataaa aaaattgatt cttatcaagt tttacgattt ttcttctttg ctttcgtgtc
    10141 caaaatacaa taaacgcatc gcgttaaaga taacgatcaa gatactagct tcgtgaacga
    10201 acatcccact agccatgtaa ataaacccgg caaataagcc gttgagtaaa aataagacga
    10261 caccaatggc aatcactaca ttttcacggg tatttaaaac agttttctta gccaagcggt
    10321 aggcatgaac caacgattca aaattagatt gcatcaagac aacgtcactc gtttcgatag
    10381 caacatccgt tcctgatccc atagcaatcc cgatatcagc cgcagccaaa gagggagaat
    10441 cattgattcc atctccaata aaagcaacgc gcaatccttg ttcttggtat tttttcacaa
    10501 attgaacctt ttcgtctggt aataattcag catggacttc atcaatgcct aacatatcag
    10561 ctacataatc agcagtcatc tgattatcac ctgtcagcat cactaactgt tttgctcctg
    10621 cttgtttcaa cttagctaat tgttcagcga cttctggtcg aatcacatct gacacaccta
    10681 tgatttgagt gatttttttg tcgattgcta caataatgac cgagctgcct gtttgttgta
    10741 attgatgtaa atcttgtact tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt
    10801 tggtgttgcc cgcacaaact gtatgaccat caatctcagc tgtgattcct tgtcccttga
    10861 ttgtctgatt atctttgact gctaattgtt gaaaatcaat gtctttctcc tgagcataat
    10921 ggatcacggc gcgccccaga ggatgatcgg ataacgtttc taatcgagcc actaaagcta
    10981 agctgtcccc ttcatcctct gtatagttct tgatagtgga tacagcagta gttcctttgg
    11041 tcaatgtacc agttttatca aagacaaaag tatcgatttt ggaaaaagta tccattgctt
    11101 caccgccttt gactaagatg ccccgtttcg ctccattacc gattcctgct acattcgaaa
    11161 caggcgcacc aatgactaaa gcgccaggac aaccaagaac caaaacagtg attgctaaac
    11221 gaaaatcacg actaatcaga ccaacaacca acgcaataag taaaacggtt ggagtataat
    11281 actgagaaaa tcgatcaatg aatttttccg catgggattt ggtatcttgt gcttcttcta
    11341 ctaattcgat aattttggca aaggtcgtat catctccgac cttagttgct tcaatcgtca
    11401 cgtagccatc tgctaaaatc gttccagaga agacttcatc tccactgatt ttgacgactt
    11461 gtcgtgattc accagtcact gcggcttcat ccgcataccc tttcccgtca atgactcgtc
    11521 cgtccacagg aatagctcca ccagttttta ctaagacgat atcgccttct tccacgtcat
    11581 cgatttccac ttcttcagtc gttccatctt cttggacaac actagcagta gatggggcca
    11641 tctcggtcaa agccttgatt gactcccgcg ttttggtcaa tgttttttgt tccagatagc
    11701 taccaaacaa aaacaagaat gtaacgatag ctgactcctt gtattctccg atcataaagg
    11761 caccaagcac agcaatagta acgagtaaat caattgagat aactttgact ttcactgcct
    11821 gatacgcatg gatcataata ggtatgaccg caataataga agctatggct agagcaatgt
    11881 tatagaacaa gacaaaacca gtaaattttc cgataaaacc aaaaataatc aacacagctg
    11941 ataaggcggt gatatgattt cgatgctctt ggatccactt ttgtaatttc atctttctcc
    12001 ctcttttcct ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga
    12061 ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt agaatacact acaaaaaata
    12121 gttagcaaga agactcacta aaagtgatct tgccaactat tgtcgtttct atcgaaattt
    12181 tcattgaatg attttcctgt acagcataca tcggagatct atttctttat ctaagatttt
    12241 tatcttgcta attttttcct ttagtctttc aaatccacgt cgttcataga attgatacgt
    12301 acagccacta tctgtaaaaa ggaatatttc ttttcctttc tctctttttt caaattctgt
    12361 tagcaacttg gtaccgatgc cttttgtttt caaatcagga tttgcagcta aaaaaatgat
    12421 ctctccatct ggcgaatgct tctttaaata tgctgcaagc atttgttcat ttgctgtctc
    12481 gtaagctcct ttactttctt tagcaaatat attttgtaaa aaatcgaata tcttaacata
    12541 tatttgctgt ccaaaagaac gatgtttctt tttttctcct tttatctccg ccagcaaaac
    12601 acctgccaac tcgtcctctg catacaaagc aagaatttga gttgctcgat tcaactccat
    12661 atacaaaaaa tatcttccgt aaagattcag cagaagttca ttttccatat atctctcaaa
    12721 gttcattcct tcaacagcaa aatagatagc ttttttgtaa tctttctttt ctaagggttt
    12781 aaaagttagt ttcatcattt cgcctcctca acttcaatga ttgtgttata cttacattat
    12841 aatagacaca gtgtctacta tctataatca atcatctata agtttgtcga ttaagcgaca
    12901 gctttgcttt gatatgtcgg ataaggagat aggtaaatga aaaagacaga tagacgcgtc
    12961 aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat tattagtaaa taaaaagatt
    13021 caagcaatca ctatacgcga actaacagaa acggcagatg tacatcgttc gactttttat
    13081 acacattata aggatatcta tgatctatat gatcaattag aaagcaactt ttttcgggat
    13141 ctaaatgaaa ttctctctta tgatccagcc catagctatg aggaactata cactcgcttg
    13201 attgactatg tttacgcaaa tactttttta tatcgcttat ttatcctcaa ccatggtgat
    13261 atttcgtttc agaagaaagt taaacaagtt attgaagaaa actatctgaa catatggcta
    13321 tatgaagaca agaaaacgat tatcacagaa gaaatgagat tctttactac gtaccacgtc
    13381 caaggttgtc tatccgttct caatcggtgg gtagaaagca attacaagta ctccaaaaaa
    13441 gaattattag aactcttaca gaaactgaat gatcacatag agaaaatcat gccttgaata
    13501 aaaacatact aagcttagtt aagaagaacc gtaaccttct cgtataacaa ctgaatctta
    13561 tttagccaaa ttgtttactg aagatttttt gagtaaaatt tctataattc ctacttattg
    13621 aaaagtagtt tgtagtgctg cttctccctc tctaagtgat atatcttgtt gtaccaatca
    13681 aattcacctc atcattaata aattactttt aggctaattt attacaactt ataggttgaa
    13741 gctgtaacca atttatagtc atattatatt tttatcatcc agtttattga attcttctcg
    13801 tttacctcaa ttcattttca aggggaatca gctcatcatc taacatttta cgccatgctc
    13861 gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct ttatatcctt
    13921 cgtaatcctt actgttga
//
LOCUS       DACDCV010000073        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDCV010000073 DACDCV010000000
VERSION     DACDCV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.073"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IV041_002089"
     CDS             complement(221..1423)
                     /locus_tag="IV041_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382848.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IV041_002090"
     CDS             complement(1426..1620)
                     /locus_tag="IV041_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8382849.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IV041_002091"
     CDS             complement(1667..2515)
                     /locus_tag="IV041_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8382850.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IV041_002092"
     CDS             complement(2720..3070)
                     /locus_tag="IV041_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382851.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IV041_002093"
     CDS             3665..4111
                     /locus_tag="IV041_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8382852.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IV041_002094"
     CDS             4735..5727
                     /locus_tag="IV041_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8382853.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IV041_002095"
     CDS             5743..6627
                     /locus_tag="IV041_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8382854.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IV041_002096"
     CDS             6676..8283
                     /locus_tag="IV041_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8382855.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IV041_002097"
     CDS             8284..9768
                     /locus_tag="IV041_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8382856.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IV041_002098"
     CDS             9945..10937
                     /locus_tag="IV041_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8382857.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IV041_002099"
     CDS             10989..11858
                     /locus_tag="IV041_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8382858.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IV041_002100"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IV041_002100"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8382859.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4258 a         2358 c         2875 g         4428 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacct
//
LOCUS       DACDCV010000074        13686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDCV010000074 DACDCV010000000
VERSION     DACDCV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.074"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(219..704)
                     /locus_tag="IV041_002101"
     CDS             complement(219..704)
                     /locus_tag="IV041_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8382860.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="IV041_002102"
     CDS             1184..1825
                     /locus_tag="IV041_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8382861.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="IV041_002103"
     CDS             1844..2479
                     /locus_tag="IV041_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8382862.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="IV041_002104"
     CDS             2497..3312
                     /locus_tag="IV041_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8382863.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="IV041_002105"
     CDS             3631..5067
                     /locus_tag="IV041_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8382864.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="IV041_002106"
     CDS             5256..7703
                     /locus_tag="IV041_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8382865.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="IV041_002107"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="IV041_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8382866.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="IV041_002108"
     CDS             complement(9193..10296)
                     /locus_tag="IV041_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8382867.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10849..11397
                     /gene="aac(6')"
                     /locus_tag="IV041_002109"
     CDS             10849..11397
                     /gene="aac(6')"
                     /locus_tag="IV041_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8382868.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11943..13604
                     /locus_tag="IV041_002110"
     CDS             11943..13604
                     /locus_tag="IV041_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8382869.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4578 a         2326 c         2929 g         3853 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10501 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
    10561 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
    10621 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
    10681 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
    10741 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
    10801 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
    10861 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
    10921 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
    10981 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
    11041 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
    11101 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
    11161 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
    11221 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
    11281 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
    11341 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
    11401 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
    11461 ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg
    11521 taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
    11581 gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
    11641 ttattcagat aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt
    11701 ttatctttgt atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga
    11761 ccatggaaaa ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa
    11821 aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa
    11881 actcttaaac aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta
    11941 aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat
    12001 gttatggcgg cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag
    12061 gaggcgtatt taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa
    12121 cagatacaat cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg
    12181 aagacagcaa accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat
    12241 taacttacaa aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag
    12301 cagcggatta cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg
    12361 cttacctgta tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat
    12421 cagaactagg gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa
    12481 caccatattt ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag
    12541 tggaagaaaa tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat
    12601 tcacattaga agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg
    12661 atcattactg ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag
    12721 aatcttctac tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg
    12781 gtgagttagc acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga
    12841 caagctatat cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa
    12901 gaaaagctat ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg
    12961 gatcagttgt ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg
    13021 aagactttgc aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat
    13081 attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt
    13141 ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact
    13201 tggatggcgt aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa
    13261 cttcgggtga ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca
    13321 gctttactga tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag
    13381 aatatgacga agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt
    13441 gggatgattt ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt
    13501 accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg
    13561 caggcgcttc atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag
    13621 catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct
    13681 aacaaa
//
LOCUS       DACDCV010000075        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDCV010000075 DACDCV010000000
VERSION     DACDCV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.075"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV041_002111"
     CDS             complement(<1..914)
                     /locus_tag="IV041_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8382870.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV041_002112"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV041_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8382871.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IV041_002113"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IV041_002113"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8382872.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IV041_002114"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IV041_002114"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8382873.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IV041_002115"
     CDS             4440..5321
                     /locus_tag="IV041_002115"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8382874.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IV041_002116"
     CDS             5318..6034
                     /locus_tag="IV041_002116"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8382875.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IV041_002117"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IV041_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8382876.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IV041_002118"
     CDS             6751..7752
                     /locus_tag="IV041_002118"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8382877.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IV041_002119"
     CDS             7749..9110
                     /locus_tag="IV041_002119"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8382878.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IV041_002120"
     CDS             9070..10221
                     /locus_tag="IV041_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8382879.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IV041_002121"
     CDS             10196..11365
                     /locus_tag="IV041_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8382880.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IV041_002122"
     CDS             11540..12919
                     /locus_tag="IV041_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8382881.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IV041_002123"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IV041_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDCV010000076        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDCV010000076 DACDCV010000000
VERSION     DACDCV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.076"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV041_002124"
     CDS             complement(14..706)
                     /locus_tag="IV041_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8382882.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV041_002125"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV041_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV041_002126"
     CDS             complement(1143..1712)
                     /locus_tag="IV041_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8382883.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV041_002127"
     CDS             complement(1786..2109)
                     /locus_tag="IV041_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382884.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV041_002128"
     CDS             complement(2263..3075)
                     /locus_tag="IV041_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382885.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV041_002129"
     CDS             3429..4277
                     /locus_tag="IV041_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8382886.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV041_002130"
     CDS             4422..5363
                     /locus_tag="IV041_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8382887.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV041_002131"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV041_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV041_002132"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV041_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8382888.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVGALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IV041_002133"
     CDS             complement(9827..12013)
                     /locus_tag="IV041_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8382889.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV041_002134"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV041_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8382890.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IV041_002135"
     CDS             complement(12235..12675)
                     /locus_tag="IV041_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8382891.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IV041_002136"
     CDS             complement(12749..13288)
                     /locus_tag="IV041_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8382892.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3764 a         2808 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagctc caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDCV010000077        13193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDCV010000077 DACDCV010000000
VERSION     DACDCV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.077"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..1821
                     /gene="yfcC"
                     /locus_tag="IV041_002137"
     CDS             82..1821
                     /gene="yfcC"
                     /locus_tag="IV041_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8382893.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1978..3162
                     /gene="iadA"
                     /locus_tag="IV041_002138"
     CDS             1978..3162
                     /gene="iadA"
                     /locus_tag="IV041_002138"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8382894.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3273..3476
                     /locus_tag="IV041_002139"
     CDS             3273..3476
                     /locus_tag="IV041_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8382895.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3577..4239)
                     /locus_tag="IV041_002140"
     CDS             complement(3577..4239)
                     /locus_tag="IV041_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382896.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4252..5754)
                     /locus_tag="IV041_002141"
     CDS             complement(4252..5754)
                     /locus_tag="IV041_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8382897.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6302..7981
                     /locus_tag="IV041_002142"
     CDS             6302..7981
                     /locus_tag="IV041_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8382898.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8108..8587
                     /locus_tag="IV041_002143"
     CDS             8108..8587
                     /locus_tag="IV041_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8382899.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8806..9486
                     /locus_tag="IV041_002144"
     CDS             8806..9486
                     /locus_tag="IV041_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8382900.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9494..10825
                     /locus_tag="IV041_002145"
     CDS             9494..10825
                     /locus_tag="IV041_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8382901.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10840..11802
                     /locus_tag="IV041_002146"
     CDS             10840..11802
                     /locus_tag="IV041_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8382902.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11913..12251
                     /locus_tag="IV041_002147"
     CDS             11913..12251
                     /locus_tag="IV041_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8382903.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12330..13037
                     /locus_tag="IV041_002148"
     CDS             12330..13037
                     /locus_tag="IV041_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8382904.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4186 a         2333 c         2898 g         3776 t
ORIGIN      
        1 tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa
       61 aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac
      121 ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca
      181 tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa
      241 gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct
      301 tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca
      361 gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa
      421 aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat
      481 cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg
      541 ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct
      601 ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct
      661 tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa
      721 actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc
      781 gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc
      841 attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc
      901 ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga
      961 atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta
     1021 gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg
     1081 acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa
     1141 aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc
     1201 gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg
     1261 atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat
     1321 gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta
     1381 ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct
     1441 ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt
     1501 gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga
     1561 caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga
     1621 caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta
     1681 tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga
     1741 ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc
     1801 attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta
     1861 gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt
     1921 ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg
     1981 aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg
     2041 attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg
     2101 gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat
     2161 ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca
     2221 ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga
     2281 gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc
     2341 tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc
     2401 atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat
     2461 caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta
     2521 ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga
     2581 aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg
     2641 cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt
     2701 ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt
     2761 gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat
     2821 ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc
     2881 ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa
     2941 aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata
     3001 ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg
     3061 gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa
     3121 aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc
     3181 ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta
     3241 gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag
     3301 atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca
     3361 agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc
     3421 gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac
     3481 cacttattac taattgaatc aagcaaataa aaaatccgaa ccatatctgc gagacagata
     3541 tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt tttgaaattt
     3601 tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct catgagtcac
     3661 gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga cgatatcttg
     3721 tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt gatgttcaca
     3781 gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt tggtcacctg
     3841 tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag caacttgtcg
     3901 ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt ctagagccga
     3961 taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt tacgataact
     4021 tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt cttttggtgc
     4081 atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat
     4141 agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg catcttgcgg
     4201 atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc tttagtttaa
     4261 taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga cagaaaacag
     4321 actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga cattgatttc
     4381 caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt gttgaccatc
     4441 gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag gagcttgctg
     4501 gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg cattcgcgac
     4561 gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag ccacttcggt
     4621 aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga cgcctaactc
     4681 atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa taccggctac
     4741 agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag gttccaacat
     4801 tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc
     4861 agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa tagcagaatc
     4921 tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag aagtgtaaag
     4981 tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg tttcgctgga
     5041 ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag tactcgtgat
     5101 tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat caatgactac
     5161 attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt ttgctgttcc
     5221 atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa agtctgcttg
     5281 agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat cagtagtttc
     5341 tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt ttgccatagc
     5401 tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta ttttttccgc
     5461 gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt cttcagtggt
     5521 agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat tggcacttaa
     5581 agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg
     5641 aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa acaataacaa
     5701 tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat tcatattttg
     5761 catccctcct agtagatgca tttgataata gacaacctat cttaaagcta gcttaaatct
     5821 tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca ctatagttat
     5881 tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag
     5941 aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat agttgttcta
     6001 acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga acagctgaat
     6061 acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt aatgaagtat
     6121 atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa ttttcctgct
     6181 gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat ggctcatata
     6241 tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag aggtgaagtt
     6301 tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa aaaacatgta
     6361 tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat atcctgaaaa
     6421 tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag aaaacattga
     6481 tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg cacttcctta
     6541 cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg agttagcaaa
     6601 attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg tggtagatga
     6661 gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca ctcatacgat
     6721 accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt atacgggaga
     6781 ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac gattggcgga
     6841 aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag aaaatcctca
     6901 gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca aatattggga
     6961 agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac aagtattgga
     7021 tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg aacgaattat
     7081 tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc ttgttacgtt
     7141 gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg gccgaatggg
     7201 tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg tcaagattga
     7261 agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa ccatcgtggc
     7321 gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct ctgagaatat
     7381 gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt tgatgaaacc
     7441 aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg cagatttggc
     7501 tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag acgtgctgga
     7561 atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg taatgattga
     7621 tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga
     7681 agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa
     7741 acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc tgatgaaaga
     7801 aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg attttgagtg
     7861 gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg aacaaacaaa
     7921 acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa ataaaaaata
     7981 aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt ttttgcgaaa
     8041 aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag
     8101 tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta
     8161 tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag
     8221 agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt
     8281 gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac
     8341 gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc
     8401 ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc
     8461 agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag
     8521 ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat
     8581 tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc
     8641 tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta
     8701 tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa
     8761 tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat
     8821 catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg
     8881 tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg
     8941 ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat
     9001 caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat
     9061 tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta
     9121 catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct
     9181 agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat
     9241 ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt
     9301 ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc
     9361 aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct
     9421 tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa
     9481 gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc
     9541 tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac
     9601 agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt
     9661 agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga
     9721 ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt
     9781 gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg
     9841 atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc
     9901 cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga
     9961 tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa
    10021 ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat
    10081 gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt
    10141 gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg
    10201 gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat
    10261 gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat
    10321 gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt
    10381 gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc
    10441 agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg
    10501 aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt
    10561 tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat
    10621 ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct
    10681 gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc
    10741 cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt
    10801 ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata
    10861 gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct
    10921 gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa
    10981 gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa
    11041 gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc
    11101 agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc
    11161 aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag
    11221 atgattgcag aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca
    11281 cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg
    11341 tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg
    11401 aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa
    11461 aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat
    11521 aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac
    11581 gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta
    11641 cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca
    11701 ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc
    11761 caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt
    11821 gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata
    11881 gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg
    11941 gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat
    12001 ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg
    12061 gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg
    12121 cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc
    12181 ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga
    12241 aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa
    12301 taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag
    12361 atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg
    12421 tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac
    12481 aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg
    12541 ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa
    12601 ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg
    12661 tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta
    12721 actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact
    12781 ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc caattggtga
    12841 cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac
    12901 tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt
    12961 atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg
    13021 gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac
    13081 cgagagttag ctgattgaat accatttatt tggaataagg gctctttgtc aataaggact
    13141 gatgggttgt acaaaatcaa atctgggtca gaatgaacct cattctggct cag
//
LOCUS       DACDCV010000078        13158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDCV010000078 DACDCV010000000
VERSION     DACDCV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.078"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1515
                     /locus_tag="IV041_002149"
     CDS             <1..1515
                     /locus_tag="IV041_002149"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8382905.1"
                     /translation="YLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELL
                     SDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQF
                     NGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFAS
                     EAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDN
                     VKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPP
                     YSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFR
                     GAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREF
                     VKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEE
                     EPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1505..2722
                     /locus_tag="IV041_002150"
     CDS             1505..2722
                     /locus_tag="IV041_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8382906.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2735..5884
                     /locus_tag="IV041_002151"
     CDS             2735..5884
                     /locus_tag="IV041_002151"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8382907.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7819..8484)
                     /locus_tag="IV041_002152"
     CDS             complement(7819..8484)
                     /locus_tag="IV041_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382908.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8610..10646
                     /locus_tag="IV041_002153"
     CDS             8610..10646
                     /locus_tag="IV041_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8382909.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10650..11288
                     /locus_tag="IV041_002154"
     CDS             10650..11288
                     /locus_tag="IV041_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8382910.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11316..11744
                     /locus_tag="IV041_002155"
     CDS             11316..11744
                     /locus_tag="IV041_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382911.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12201..13112
                     /locus_tag="IV041_002156"
     CDS             12201..13112
                     /locus_tag="IV041_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8382912.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         4567 a         2079 c         2560 g         3952 t
ORIGIN      
        1 tatttattgg gactgatttt ctataaatat ttatccgatc gattgttgga acaagtggtt
       61 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac aatgttgtat
      121 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgccacaat cgttgatatt
      181 ttgggatatg caattgcacc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
      241 gcaacgttcc aattgaacga tctaaacaaa gcctttgtgc aattggcatc tacctacaat
      301 caatttaatg ggttatttga tgatgtggat ctccaatcaa aaaaactagg aacagatgag
      361 caacaacgaa atgtgacgat taccgaagtc atcaagaaat tgaatgatgt agatgtttta
      421 gaacacgatg gggacgtgat cggggatgcg tatgagtttc tgatcagtca atttgcttca
      481 gaagcaggga agaaagccgg agaattttac acacctcata tggtttctga tatgatggcg
      541 caaatcgtaa cgctcgatca aaaggaacga cgattcttta gtgtttttga tccaacgatg
      601 ggttcaggtt cattgatgtt aaacgtacgt aactacttaa cacacccaga taacgttaaa
      661 tatcatgggc aagaactaaa tacaacgaca tacaatctag caaaaatgaa cttgatttta
      721 catggtgtcg acgccgaaga aatgaatctc cgcaatgggg atacgttgaa taaggactgg
      781 ccaacagatg aaccttacac atttgatgca gtagtaatga acccacctta ttcggctaat
      841 tggtcagcag atacgacttt cttagatgat tctcggttta atcgttatgg gaaattagcg
      901 ccaaaatcca aagcagattt tgcgtttctt cttcatgggt tctatcattt gaaggaaaca
      961 ggtaccatgg ccatcgtctt accacacgga gttttattcc gtggcgctgc agaaggggtc
     1021 atccgtcaaa aattactaga agatggcagt atttatgctg tgatcggtat gcctgcgaac
     1081 ttgttctttg ggacatcgat tccaacgacg gtgattgtgt tgaagaagaa tcgtcaaact
     1141 cgagatgtat tattcattga tgctagtcgt gaatttgtta aagggaaaaa tcaaaataaa
     1201 ctatcggaag aaaacattca aaaaatcctc gaaacctacg ctgaaagaaa agatgtcgag
     1261 aaatacgctc atttggcaac ctttgatgaa atcaaagaaa atgactataa cttaaatatc
     1321 ccacgctatg tagatacttt cgaagaagaa gaaccaattg atatggttca tgtcggaaat
     1381 gacatcaaga agatacgaca agaacagcaa gtgctagaaa aaaaactttt agaagctatc
     1441 tcttccctgc aaacaacacc tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc
     1501 aaacatgagc aatgatacac aaccagaaat ccgtttccca ggatttacag atgattggga
     1561 acagaggaag ttgggggact tcgttgtaga ttatgttgaa aagacaagtg tacaaaatca
     1621 atttcctatg ctaacttcat ctcaacaaaa agggatagtt ttgcaagaag actattttgc
     1681 aaatcggcaa gtaactacag aaaataatat tggttacttt gtactaccta gaggatattt
     1741 cacgttccgt agtcgaagtg ataatgatgt gtttgtgttt aaccgtaatg acattattga
     1801 tagagggatt ataagctatt tttatcctgt atttacttta aaatctgctg attccgactt
     1861 cttcttaaga cgtattaaca atggaatcca acgacaatta tctattcaag cagagggaac
     1921 aggtcaacat gttctgtctc taaaaaagtt taaaaatata gtagcaatgt ttcctagtga
     1981 ggaagaacaa caaaaaatcg gcaccttctt caaacaactt gacgacacga tcgctcttca
     2041 tcagcgtaag ttagatttgc tgaaggaaac gaaaaaagga ttcttacaaa aaatgttccc
     2101 tgaaaacgga gcaaaagtgc cagaaatccg gtttcctggt tttacggaag attgggaaga
     2161 gcgtaagttg ggagaattag gtaaaactca atcgggaatt ggatttcctg atgccgagca
     2221 aggtggttct gaaggtactc cattttttaa agtttccgac atgaataaca ttggtaatga
     2281 gtatgaaatg agaaatgcaa atcattatgt atctaatgag caaattgagc gtaaaaaatg
     2341 gaagccaata aaagatgtgc cagcgattat atttgctaaa gttggcgcag caataatgct
     2401 taatagaaaa cgtttggtca caagcccttt cttaatagat aacaatacca tggcttattt
     2461 atttaataat acttgggata tctattttgg taaaatattg tttgaaacta taaatttgcc
     2521 aagatactcc caagtagggg cattgccttc ttataacagt tctgatattg agaatatcag
     2581 tgttaaggtt cctgtaaagg atgaacaaca aaaaatcggt actttcttca aacagctaga
     2641 tgatacgatc gctcttcatc aacgtaagtt agatttacta aaagaaacga aaaaaagctt
     2701 cttacaaaag atgtttgtct aggagggaag aagaatgaaa attagtcacc gtgaggaagc
     2761 agaagtcgaa gaacagttga ttcgtgtact tggagaaggg cataatcagt ggacgtatcg
     2821 tccggatcta aaatcagaag aagacctttg ggtcaattta cgccaaaaaa ttatctcaaa
     2881 taatcaagca gaactgaatg attttccttt gaccgataaa gaatttgaaa cgatcaaaac
     2941 ggaattactg ctgcgaacga aaaccccatt tgatgctgcg aaatggttaa aaggtgaaaa
     3001 tgggatggct cggatcacga ttgaacggga agattcgcaa ttgggttctg tctcacttat
     3061 cctttattcc aatcaagaca ttggtggagg aatctctact tatgaagtgg tccatcaaat
     3121 tgcaaaacgt ggaagtaaca tagaagcacg ggatcgacgt tttgacgtca ccttattaat
     3181 caatggatta ccgattgtcc aaatcgagct gaaacaagta actgcaaaag atggtgttta
     3241 tcaggcattt aaccaaatca aaaaatatgc agaagaaggt atgtttcgga acaatatctt
     3301 ttcgacctta cagttgtttg tggtttccaa tgaacaaacc actcggtatt ttgcaaatgc
     3361 cttaccgaaa gacttgcacc ctaagttttt atttagttgg cgaacgaaag acaatgaaaa
     3421 agtagaaaac ttatatgagt tttgtaaaca agtgttaaat atcccggatg ctcatcgttt
     3481 gattgcggat tatacaatcg tcagtgaaga tcaagacaat aaaaccctca tggtgttaca
     3541 tccttatcaa gtacatgcga ttcaagcctt gtttattgcc gcaaataaac atcaatcagg
     3601 gtatgtgtgg catgcgactg gttcaggaaa gacgttgact agttttgtat ctactaagct
     3661 tttagcacga aaatcgggga tcgatcgaac gatcatgtta gtggaccgca aagatttaga
     3721 taatcaaacc accacagagt ttacaaaatt tgcttcggaa tttaatactg ggatttcttc
     3781 cgggaacgcc aaagcgaata gcttgatcgt tggaacggga agtgccaaag aattaagtga
     3841 aaccctgttg gcagatgcca atgcaaatgt tgtaatcatt acaacccgtc aaaaactaga
     3901 tgcggcgttg aaatatgcaa agaaacaaga agagaaaaaa ggaaccaatc gcttccaaaa
     3961 attaatgggc caacatatcg tgtttgtagt agatgagtgt catcgagcgt taagtgctga
     4021 aaatatggaa gaaatcaaaa aaatgtttcc gaaatccact tggtttggct ttacaggtac
     4081 accgattttt cctgagaatc ggaaacaagc caaagggcaa ttggcacgta caacccatga
     4141 tcagtatgga gaggtcttgc atacctatac cattaagaat gcgctagaag atggctctgt
     4201 cttaggattt caagtagagc atgaaaacac cgtggagccg acttcactag agaataaaat
     4261 ctatcggaaa ttaaaagaag tagaaacata tgcagagtat tcatcagaac agatcaatcg
     4321 tatgattgat caaatggaac ctgtcaaaaa agaaagttat ttggacccag ctgtttttga
     4381 agcagatgaa catatccaaa aagtcattca taaaatgttc cgtccagata atgcctatac
     4441 aaaatttgat tttcgaaatg gcaggccaac gaaatctgca atcttaacca cgagttccat
     4501 tgacatggcc aaaaagtatt atcgggccat caaagagatg acgaaagagc ctgattggtt
     4561 aacaaaagag tttcccaatc agctcattcg tgaaggacga acgatggagg atcctgattt
     4621 tccacgaatc gctattacct attcattaga agaaaatagc cgagattcaa gtgcccaaca
     4681 agaagaaatg caaaaaatta ttgaggaata taatcagtat tatgatactt cctggtcatt
     4741 agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa aaagagcgga
     4801 attcaaacaa tttgggaaac aaatcgattt agcgattgta gtggatcgtc tattaacagg
     4861 ttttgacgcc cctacgattc aaaccctatt tgtggatcgt aatctagagt atgcaggatt
     4921 gatccaagct ttttcacgta cgaatcgtac gtatcctgat aagacaaaag gattgattgt
     4981 gacgtttcgt aagccagcga caatggagaa gaatgtcgag gatgcgacaa aactgtattc
     5041 aaaagcaaaa gaagaatcag gtttgattta tccaagttat caggaatcca aaaaaagatt
     5101 caaacaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg atgaacatac
     5161 gccacttgaa atccgtatcg agtacgtcaa agcatttcaa gaattgaatc gtgcttatga
     5221 atcgttagtg acttatgacg attacaatga tgacatggtg aaatctccaa ctttgaacaa
     5281 tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg gttcattaat
     5341 agaggaagaa ccagaaaaag aaggagaaga tttttcaacg atcgaatttt atagtgaaaa
     5401 taccactaag ttgtatgata ttgattcaac ttatattgat caattactag gaacttatgc
     5461 tgcgaatagt tcagatgcac gagaagaaat tgaaaaagct ttggctaaat tgaataaaac
     5521 agaaggagtc aaagaagttt accgacaaat tttaaatgca tttgatgtgg ggactttaga
     5581 tagtaatgaa gatatctttg tcattaaacg tcgctatttt acacaagcga gtgatgattt
     5641 gattcatgtc ttttccaatg agtggtttgt ttctgagaaa gaattacatg cttctaatct
     5701 gcaatatcta ccaggtgaag atccaattcc taatatgaaa gcaattatca acagtaagga
     5761 ttacgaagga tataaagcca aacatccaga agcgaagcca tttaaatacc cacaagaaat
     5821 gaagcgagca tggcgtaaaa tgttagatga tgagttgatt ccacttgaga atgaattgag
     5881 gtaaacgaga agagttaaga aaattgtacg attcaaagat ggtagtcaag tggaagtgta
     5941 gaaaataggg atagatgtct tttatttgtt cacattttat aacgaagtat ttaagacagc
     6001 tattgaataa tgtttcctcc acctttttca ttcatttacc ccaccccgtt gttatatgac
     6061 cgtgttttga gaggaaccaa taaaaagcat aggctaacta ggggttcctt tttcttttgt
     6121 ttttttggaa tccgcttaag tgaaatgtat ctgaaatgaa acggagataa agtgattttt
     6181 tgctataatg acaaggtttt agctgtttca gataccatgt aacccaaaat agtttattga
     6241 gaaaatagga gagtatttga tactaactag taggaaaaaa tgagataaaa attaaatgga
     6301 atcatttcag gtaatctcaa ataaaaaaat atacagtcct gtggcacttt acctatggga
     6361 ctgtatattt tttactctca gcgggcttgt tttgactccg tgtctgactc cgtcgtattg
     6421 attttaaatg atattcgatg accatttaac aggtcatttt tgaaattttt gtctctatat
     6481 gactcagttt gataattatt gatagatcaa tttgggtcta tatcgaacaa gggaataatc
     6541 caataaaaaa tgatttagaa tatcaaaata acaaaaaaga ggactacctg tattcgtcct
     6601 cttttttgac cagattttga ccagtctaat ttacattcta atatattcat atgagctaat
     6661 tttttgatta aatggtaagt ttttccttgt ttgttttagt ttaaactagt gtgtaattta
     6721 aaaatctata tggaagggtc tgtggggtaa tacttaaata aaggctagga acctgataac
     6781 atcaaggttc ctagcctttt tataataaaa tgaagcgaaa ataatttctt ttaaagagcc
     6841 gagattatca gctatataag agtgaatttg atgcaaaaaa cagagccgaa acagtcttgg
     6901 tgttcgtagc tctgcttata tgtttcgaat ttaagacaaa gagttggcaa aagatgttga
     6961 taaggaagat aagataactg tgttatataa actaggtaac taactgtaac aagctaattt
     7021 taagagattc ttattagaag acgtttagga atataactaa gaaaatgatt atacaggtaa
     7081 ttgtcatttc aataatgttt tctaattttc gcgtctttgt tttaagaaaa aggccagaat
     7141 cagttggaaa aaagttcgta cgatagtgaa gaggggcaaa atttcctact aatattccca
     7201 ctaacaagag ataaaacggc caggttggtg tgattcctct ataaaaaaat gcccatgcta
     7261 ccgaaatccc atagattaac aactgaacaa tttttacaat gaaaaaattg ttctgccatt
     7321 actcaaagtc tcttcctcct tgtatcgttt atttgtttca ttttatcatt atgataggcg
     7381 cttgaacaat ccattttgta ttttattgag tactcttgga taaactacca gcgattatca
     7441 tatttattta tgttactgta agccatcata taaaagtaaa tgagcagatc atagaaatat
     7501 aaaaaagaac taaaattgat ctaaattatg tgcagcttga ctacgtacca atctatatta
     7561 cttactagat tggtcgttaa tctattcgga tcgaatgagc aaaatatttt tgtgaatgtc
     7621 catccttgat tatgattcca aaaagaatca ttgggaggct gtggcgtaaa tattatacta
     7681 taagccagaa atacctatca ttctgaactt ttttatttag gaactttcat ctcaaggctt
     7741 tttcaattaa tttgtacccc tgttttttgg ataagatcat gtgcattgat tttttctatc
     7801 tctacgttca tctcctactt aatgcctatc tggctcctga gggcttcttt ttaaggcgtc
     7861 gtcatagtct tggctgagga tttcttggta atctgaggga atggcttccc aagtgagcag
     7921 gcgaacgttc atgacgtgtt ttcctttgtg tttgattaca gcatggtttt tctctttttc
     7981 tacctctcca gcgtgaaaag acagccatgt accatggcca aagatatcat tacataccca
     8041 acgttcgatg gaattgtcca cgtatcctac agggagtgtg gtatagccga tttgtggttt
     8101 caaaaaagga ttgaatcgat cgatcgtttc atttttgtat tgataattga taagaaaaag
     8161 atagaccaaa caaaagacga gcagaaagaa agatagtgtc tttttgagtg agaagggttg
     8221 ttttgatgaa taagatttct tgttaacaag aggttcatct ggcaatgttg gttctattga
     8281 ttcggtagct gattcaatag aaggaaaatc aatcggcaga cctaaagtat gatgtaaaag
     8341 tgacagcatt tcaaggtctg gcattgtacg agaattttcc caactagaaa tcgttttatc
     8401 ggaaacacca attttttcgc ctaattcttt ttgcgtaagc ttacaatttc tgcggtaagt
     8461 tcgtattttt tctccggtgt tcatcttctt ctcctttcga ttctacagta tatagattta
     8521 ttctacatga cctatacact tgatagatac gtttttttga tgaaaaacgg tatgatttta
     8581 tcaaagaatc atttaagtaa gggagagtgg tgataatcaa aaaaaagttc acagttttac
     8641 tcacaatcat agtgaccggt atcatgagtg gttttgcctt ggttagtata aataaacagg
     8701 tactctatcg gacaaatgaa tttttccatc aaacagatac ttatactccg attcgattgc
     8761 cacaacattt tattgggcaa gaagaatatg agcagctaat catggtattt gaaaatgtct
     8821 catcagaaac tgagctttct tatatgaaaa aaacagtaaa tgaatccaat gaagtagata
     8881 catcttggaa cttcaagata cagccaaagc ttgaaatgac attgtattat caaggggatg
     8941 tcaaatcttc tcacgagaag gtaccctatt ctaatacctc ctttcgcgta acgtccattg
     9001 aagaatcgat caaaataaaa gattttgaag gtgaattttt cttgatgacg agagaaaaag
     9061 aagcctatga acggttcatt tcactattca ctgatcgatt caatcaagca tttcacactg
     9121 attatgccac taatattttt gaagactttg aaaatagcta ttcgtattcg gttatttcaa
     9181 atacagacgt atataattat cgagtgtatt taactattgg attgattctt ttattccttt
     9241 tgttatcatc ctttttctta ttgatgaata aagaaatcag tttattgttg aataatgact
     9301 tttcattaca tcaagtattg tggtgtttat tggggaaaaa gaaaatcatt attacattca
     9361 taattgcagg aatcattggt ggttttattt ttatgtatta taaggagcaa tttctttatt
     9421 tgttccagat gtacgttttc ttgttcagcg gaatatatgg tatctctttg atttctcttt
     9481 tattagttga aaaaatcgct tcgttaagag aagcgttcaa ctatatccaa tacattcttt
     9541 ttacatttgg attgttgttt tctcttcttt tgaccgttgc caatgtcgaa ttagcaacta
     9601 tcgttttttc ttcttcacag ttagctgttt cccatgaacc accaaaagaa atcgctggtg
     9661 aggactatca tgtattttat ccagtaacca tcgggaaaaa ccacgtggat tttatttatt
     9721 ccaatcgttt tgcatcagca gcagatcagg aattatatga aactttgaat aaaaacggaa
     9781 gtatattagt caatgtatca gattacttga atgaagaaaa tgaacagttt ggcagaggga
     9841 taaaaataaa tctgaattat cttaagaaat ttcctttgat tgatgtctct ggaaggttga
     9901 tccaaataac cgaaaaagaa acgcaccctg ttatattgat ccctgaacga tatagacata
     9961 cagatttaaa aaatgacctg gcgcaaataa cagaatatta tcaagaaatc agtgaaaaag
    10021 aaccgaaatt tctatttata aaaaacaatc aacccattta tacatttatt ccttctatcc
    10081 cctggatcga acattatccg gttgttgaaa tactgacatt gaaaaacagt atgtatttgg
    10141 aaagaaatat tctcagcgga gaaatctatc caccgttaaa gatcaagata ggctctttgt
    10201 cgaaagaaag gttatttgag ctgattgaag aaagtcagtt acgtgataac ttgcagttat
    10261 catttccgta tactcagact gaagagatag ctgtcaaagt gttaagcaga tcatttcatt
    10321 acttgataca gatattttta ctcactttct tttctttctt cttgttgagc tatgtcatgt
    10381 tatgcatata ttttgtctac aattgtcgga aaatcgctat ctttcgttca agtggttatt
    10441 cgctctttga aacgtataaa gatttcttca tgatgaacct catcaaatgg ggaacgacaa
    10501 gtgtaatctt cctttttctt atagaaagag agcctaaata tctttttaat atttttttct
    10561 ttagcttcat tgattttatt ctttcagttg tttttatttt gtctacagaa aagaaaagtc
    10621 agttattgct gatgaatgga ggttaacaat tggagccgtt gatagtgata aaaggtgcga
    10681 aaaaaatcat tcgaaacaaa acgattcttc atttggatcg tttcactgcg gatgctgggg
    10741 atcttgtgac tgttgttgga gaaagtggag caggtaaaag tacgttattg aatatattag
    10801 gattgaatga tcagttcagt tcaggacaat tctatttgtt cgatcaagag acgaaacaat
    10861 taactgggag agagaagcgc ttgataaaaa gaaataccat atcttttctt tttcaagatt
    10921 ttgggttgat tgaatatgaa acaatcgatt ttaatttaga tattggattg aagttttcga
    10981 atttaaatgt ttcagataag cgaacagcaa agtatgaggc attgaaaaaa gtaaatataa
    11041 acaagaagct aagtacgcct atctacacat tatctggtgg tgagaagcaa agaatcgcct
    11101 gtgcgagagt cttattgaag aatagcaaaa tcattttagc cgatgaaccg actgggtcat
    11161 tggataaaag aaataaggat aaagtagttg aaatcttatc gcaacaagct tcccaaggta
    11221 cgttagttat cgtagtcacg catgatccat atctgaccaa aataggaaac aagaagatca
    11281 tgctttaaaa atcaaaaagg aaaggatgtc gttttatgaa aaaagcagtt attatttgtg
    11341 cactagggat aggttttgtt ggaggaaacg ctatgtcatc tgaagtggaa gcaaattcag
    11401 ttcacggtga aatctattac aaatctttaa agccaggaga gattcatttt gatgctaaag
    11461 gtaatattat taagtcaaat ttaccagtag accgggaaat ttctagaaca gcaattaatc
    11521 atagctctgg aagatgggtt tattatagtg atacaacttg tacgagaaaa acaggtcatt
    11581 ctaaccacta tagtcgtgtc tataatcgtc acggtgcaaa agcgaaagtt ggaaactcta
    11641 ttcgatcagc aaatgcaaca aaaggagtat gggcctatgc aagtgcgaca ggaaatagaa
    11701 ctgacacatt ctcatgttgg tacaatccaa ccggttggta ttaagtatcc aaaagtttat
    11761 ataaaataga aaaaacatat aatgaaacag cgttaaaagt tatttttggt agttttaata
    11821 tattcatttt tagttgtata aaaatatatc ataataaaaa caacaaaaaa atgtctggaa
    11881 aatcgaacat aaaagtgtct gatttgaata tcccagcagc ggaagaagaa aaacaaaatc
    11941 gataacattt gttctaagaa ttagttacgt taaatacgga acgcaagata gacttgaaaa
    12001 acggtaacaa aagaagtagt atattaaggt gtgatgtgct acttgacatt tattctgctg
    12061 agcgaacttt ctggattgtg tagccttata tctattcagg acgtatcgag cagattagta
    12121 aaggtgttct tgatgatttt catcttgaac acctttgttt ttagcaattc gcaaaaaacg
    12181 aaaagtagga gaattgataa atgaaaaaaa gatttttagc acttgccatc gtactaggca
    12241 ctggcttatt gtcaggctgt acgaatgccg gtgaaaaaac agccgttagt tataaaggcg
    12301 gtacaatcag cgaacaagaa gtaatggata gcttgaaaaa aatgcaagga gcagattccg
    12361 ctgtgcaaca gttgatcgtt tatcaagtat ttgaagataa atatggagat gacgtatcaa
    12421 ccaaagaaat cgattctcaa tatgatcaaa caaagaaaca attgggtgat tcatttgatt
    12481 cacaattgaa atctgcaggt tacacagaac aaacattcaa agacagtatc aaacaaagtc
    12541 tagcctttca agaaggtcta aagaaacata tcaaattgac cgatgaagat ttaaaaacgg
    12601 cttgggaatc tttccatcca gaagtggaag ctcaaattat ccaagtagca agtgaagatg
    12661 atgcaaaaga tgtgaagaaa gctgctgata agggagacga tttctctaaa ttagccaaag
    12721 acaagtcaac tgatactacg acaaaagaag atggcggaaa agtgaaattt gattccacta
    12781 ctacaacggt tccggcagaa gtcaaagaag cagcctttaa gttgaaagat ggtcaagttt
    12841 ctgatgtgat cacttctacc aatgcttcaa cttatactac ggaatattat gtggttaaaa
    12901 tggtgaaaaa ccaaaacaaa ggcaacgaca tggataaata caaaaaagaa ttgaaagaaa
    12961 ttgcaacaga cacaaaatta agtgacagta ccttccagaa taaagtaatt ggcgaagtat
    13021 tgaaagacgc taatgtaaaa atcaaagaca aagactttga aaatgttttg agtacattca
    13081 cttcagattc tagtacagca agtagtaaat aagcggtata aataaaaaat atgaactatt
    13141 taaatcaata aaactgtt
//
LOCUS       DACDCV010000079        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDCV010000079 DACDCV010000000
VERSION     DACDCV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.079"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV041_002157"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV041_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8382913.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV041_002158"
     CDS             complement(322..891)
                     /locus_tag="IV041_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8382914.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV041_002159"
     CDS             1077..1979
                     /locus_tag="IV041_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8382915.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV041_002160"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV041_002160"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8382916.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV041_002161"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV041_002161"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8382917.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV041_002162"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV041_002162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8382918.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV041_002163"
     CDS             8547..8732
                     /locus_tag="IV041_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8382919.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV041_002164"
     CDS             complement(8794..9990)
                     /locus_tag="IV041_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8382920.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV041_002165"
     CDS             complement(10160..11596)
                     /locus_tag="IV041_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8382921.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV041_002166"
     CDS             11889..12698
                     /locus_tag="IV041_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8382922.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDCV010000080        12287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDCV010000080 DACDCV010000000
VERSION     DACDCV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.080"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(104..667)
                     /locus_tag="IV041_002167"
     CDS             complement(104..667)
                     /locus_tag="IV041_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8382923.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(682..1725)
                     /locus_tag="IV041_002168"
     CDS             complement(682..1725)
                     /locus_tag="IV041_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382924.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1858..2262)
                     /locus_tag="IV041_002169"
     CDS             complement(1858..2262)
                     /locus_tag="IV041_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8382925.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2495..3130)
                     /locus_tag="IV041_002170"
     CDS             complement(2495..3130)
                     /locus_tag="IV041_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8382926.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3396..4448)
                     /locus_tag="IV041_002171"
     CDS             complement(3396..4448)
                     /locus_tag="IV041_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382927.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4461..5399)
                     /locus_tag="IV041_002172"
     CDS             complement(4461..5399)
                     /locus_tag="IV041_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8382928.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5850..6500)
                     /locus_tag="IV041_002173"
     CDS             complement(5850..6500)
                     /locus_tag="IV041_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382929.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6600..7109)
                     /locus_tag="IV041_002174"
     CDS             complement(6600..7109)
                     /locus_tag="IV041_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8382930.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(7310..8179)
                     /locus_tag="IV041_002175"
     CDS             complement(7310..8179)
                     /locus_tag="IV041_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8382931.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8199..9320)
                     /locus_tag="IV041_002176"
     CDS             complement(8199..9320)
                     /locus_tag="IV041_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8382932.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9322..9717)
                     /locus_tag="IV041_002177"
     CDS             complement(9322..9717)
                     /locus_tag="IV041_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8382933.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(9736..10533)
                     /locus_tag="IV041_002178"
     CDS             complement(9736..10533)
                     /locus_tag="IV041_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8382934.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10535..11323)
                     /locus_tag="IV041_002179"
     CDS             complement(10535..11323)
                     /locus_tag="IV041_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8382935.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11337..11813)
                     /locus_tag="IV041_002180"
     CDS             complement(11337..11813)
                     /locus_tag="IV041_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8382936.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(11885..>12287)
                     /locus_tag="IV041_002181"
     CDS             complement(11885..>12287)
                     /locus_tag="IV041_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8382937.1"
                     /translation="QLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVS
                     DAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTH
                     FKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         3844 a         2614 c         1846 g         3983 t
ORIGIN      
        1 cgtaataata ttaaaacact agaacaaatc aattaaattc cataaaatat aaataataga
       61 cctagcatca tctggtataa aatggatgct aggtccgtta tatttactca aaattgattc
      121 tacgaataga tcttcttatc atttctattt tagtagtcat gcgttgttgt tcttctaaag
      181 gtggtaatgg tataagtagt ttagataaat ttgtaccatt tacatttggt tgcccagttc
      241 cgtaagaaat tttttctaat aacttccaat ataatggcga gtctaagaag cagtctacgt
      301 attctgataa taatttctca actaacctaa ttcgaatcaa gtaagaagca aaaacagatt
      361 cctcacttat ttccttaacc aagaaacttt ttcccatagt tcctcctgtc cttgctatca
      421 aaatgtcatt ttcttccaat cttaaatcaa ctagtttaga attactaata tcacagtaag
      481 gcacactgga ccaatttaca cgtccctctt gtatatctgt gattctcaaa aacttaacat
      541 tgccttgttt tttcgccgaa tctgtatagc catattgaat cttagtagaa atacttccta
      601 aataaaccca tagtcgaaca tcgctagatg atttttcttg tcgttcttta tgaacaacag
      661 tgatcatctt gagttgtccg actatttttt agttgcagca atctttttgg gacgcttatt
      721 tgcatgaagc aacgttgcta agtctagtaa taaaacattg gctaacacag gattggcttt
      781 ctcgcgatac gctgcggcgc cactttcaaa caatactgct agacgatctt ctacaacaac
      841 gatttgttcc aaatacggtg gaaatttgat tcgttttagg atccggctag gagagtcccg
      901 ctcggacact aagagttccg aggtataagg accataagaa cgactggcta agagataatg
      961 gccataaaaa gccacacctt gaatcttatc agatgttttt ctgctcattg tcacggttgg
     1021 ttggattttg tctttgcggc gaattgctgt tttcatttca gctatcgtcg gcaatagata
     1081 gaaagtcaac tgtccttcac cttttagtgt gaagttgcca gataccagtt gatcattgtt
     1141 ttgagtcaaa tacgatgctt gtggaatctc tgagagatca gttgtcagcc aataagcgat
     1201 tggctgtccc gtctcttcag atgtatctgc taagatatcg atcaaacgta aagcggctac
     1261 tcgtccagta gcagatcctg tgattgtcaa ccataaaagt tcccgttttg tatcatatac
     1321 taagccccct acatgtggcc gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc
     1381 tctatccagt acatgaataa tagaatgatg ctgatggtca tagcagtagg cagaaataaa
     1441 cacaaaatct tgagaaaaag ccaacccttg cggcgtcatc cattgacaaa cctcattttc
     1501 gccggtttcc ggcaccacca ttcttgtttc tgttaatcca ggaatgttat attgttttgc
     1561 ttcagccttt tcccaagctg cttgaacaat aggataccgt tttaactttt tttcaatatc
     1621 ctgatgtatc cgttgctgct tccaatgctt aaactttagg acatagtaaa caagaaggcc
     1681 aatgaccagc aacactaaaa tcattattgc tacccgctgt gtcatttttt tgcctccttg
     1741 ttctattcga tcaatcgtcc atataccgct attaattacc gtaactacat gtttctttta
     1801 ctagcgtttc tttataggat ctttcataat ctcatttata gtaacattaa gaatccctta
     1861 ttctactata gtcacttcca catcaatatt gcctcttgtt gctttagagt atgggcaaaa
     1921 tgcgtgggct tcttctgcga ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac
     1981 ttctaaaacg accccgattt ggtagccccc atcttctaat tgaaataaag atacccgtgc
     2041 tttgacttca gaatcagact caagattatt ttcttccagc atgtgttgaa ccgcgccgtt
     2101 gaagcaagat gcgtatccag ccgcaaaaag ttgttccgga tttgtccctt cactatggat
     2161 tcctgggcgg tcgatcttca tgtgcatact tccgtttggt gcctcaactt cgccatcacg
     2221 gcctccacga ttgatcattg ctgtttcgta catttttttc attgagatca tcctttcttt
     2281 cgtcgttcta tattaagtgt aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt
     2341 cggcgacatc agttagacaa catcgatgat cagtgataga agcagtttat ctatacaaaa
     2401 aaagaagggt tctatgatat ttggtgtcct tactcgcctt gagtaacaac accaaatatc
     2461 atagaaccac ttaatcagct tcacactgct ttttctactt tcgtagttga aaaaaactta
     2521 aacaaatcat cggcaaatag ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct
     2581 taaagaaggc aaactgacta atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta
     2641 gtaaactaat aactgttaaa acaagataaa aatggctaag cgtaccttga ccactttgac
     2701 gataaagcag aacaagtaac aatgggaaaa gtagaaagcc tgcatagcct agcccagaac
     2761 ctatattatg gatccaagtc gatagattac aagagctttc attcgtattg atactgaata
     2821 agccagtaaa aatacaatca cctatcccat ataaaccaac tgttgcaaca acaagtattg
     2881 caagtgtttt tgatgttcca acaaataggt gatacaacgc aggcaacgaa agaacaaaaa
     2941 aaagtccaga tacaactgac cagaccaaga aagcacgtct gactggacta tctacgtcac
     3001 caaacacact aattacggta gtcatttgat tcagtttggg ataaaaaagg ccaaggatat
     3061 acggtgtcga taaatcactc atcacaccta ataataaaaa ataaaaacca tatttttgca
     3121 aaaaaatcat tttcttcccc tactttttag tgattattcg atacatccaa caatcaaatt
     3181 ttttctcatt ttaacacata tttttattct agttgtcttt tgaacaatca ataacataaa
     3241 ggtgagtttc ttctacattt taaaaaatcg aatggcactg aagagttcag ttgtcagcat
     3301 caatctccta ctcaaaaagg caaaaaaacg taagtcagat gttgttgaca tctcacttac
     3361 gcatccattg attgaaaatt tcttagtaaa atctactagc ctaaaaattt gaagacttct
     3421 tctcgatcat acagtttaaa tactttctcg cataaaaatt gagtaattag tgccgcaaaa
     3481 acagggatga ctaaccaaca aagaacaaca agaaagccat ttagccctgc atctaaagaa
     3541 gccaacggac caacaagtcc gaccacgccg aaaccagcag aagcaggcgt acctgaaata
     3601 gaaaatagag cgacaggaat agctgtaatc actgcactaa ctaaacaagg aataagaatc
     3661 acaggttttc taaatagatt aggcatcatc atcttcatag cgcctaaagc aatcgcaatt
     3721 gtcacacctg acttattaac cttccaagag ttcactacta aaacaaaagt ggttgcagct
     3781 acgcccatcg cagccgcacc agcagaaaca ccatctagtt ggattgccat tccgattgct
     3841 actgttgaaa taggagagat aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt
     3901 aacagcggtt gtaaggtagt gaagttatta atcaatgatc caattgcaga agtaattgct
     3961 gttacaaaag ggaataaata aaaaccaagt aaacctgcac ctacacctac aacaatcggt
     4021 gttaacacaa tcgacactga cccaaaacta tttccaatca gtaaaatcag cccgacagca
     4081 atcgatgctg tcagcatcgt attaataata tcgcctgtac cagcaccaac gtaaacaccc
     4141 gtttcaccca aatcaggtgt gaatttgact acaccagaac caacaaaaga agctccggct
     4201 acaaccatca ttctcatcgg atcaaatcca aattgtaagg caatcaaacc accaatgatc
     4261 aaaggtgttg ctagttgaaa aataactgct aactgtgcga tcgttaccgc aaagggatac
     4321 gcactaaaat attttaaaat ggcacctaat actgcattag gaatcaatcc aataatgatt
     4381 cctgttgctg taccagcgag gactttattc agaaaaattt tcggtgatat tttttgtgaa
     4441 gtttccattc aatctctcct ttattttacg ttcaaactat cttcttttgc atggacaagc
     4501 tcagtcaaga aatcacaata aggcgtcgca acaccatatt tcttcccttt tctagaaatt
     4561 gcgccattga tataatcgat ttctgttaat cgatggttct taatcaagtc ttgatacatt
     4621 gaaggataat gtaacccaat tgtttccggg tcaaaacaag attcacaatg tgcaatgact
     4681 tctgggacat ctagttcaat cttctctact gctgctactt tggcaaactc attgacaatc
     4741 gtcgccacca ttttatgagc agtcgatgtt tttcctagct cagccatatt aacgtctaag
     4801 attgtgcata gtccgttcat tgttccgttc acacatgctt tgcgataaat agaatagtga
     4861 atattgtcag aaaaatgagc atttaatcct gattcagaca atttgtctgc taattttttt
     4921 gcagctgctt cttttccatc acctaaattt tgtaattcta ctgaaccact tccaaataat
     4981 ttgacctgac caggaccttc aagacctgcc gtccacatgg tatttccaat atagatattt
     5041 tccattggta caaatttctc aataatatct tcatgcccga taccatttaa tagacataag
     5101 acctctgtat cttttttgat taatgattgg atatcctgca gcattttttc cagctgcatc
     5161 gctttggtaa atagaataat cagatcaact tgatcttctt tttctacttc ggattgaaga
     5221 acgattggta gttttgcttc tacctcttta ccattaaaat ttgcttgtaa tccatgttct
     5281 ttgatttgtt ggacatgttc tgcccagcca tcgattaata atacttcgtt cccactttgg
     5341 tgaagcatca acccaaagcg acttcccatt gcgccagcgc ctgcaattgc tattttcatt
     5401 gattcttcct cctttttctt tctattatac tcatttgaga atcttttcac aacctcgttt
     5461 tgttattctt ctcttattta tcatgtttat atccaccatt caagttattt tatcagcaaa
     5521 tcaataaaaa ctggaataga atcccaattt catcgtaaga gaaactagac tagcaataag
     5581 aaaatagaaa cattcataga aaaaatctca ctcctctgat tataaaagag ggggcattaa
     5641 cctcgtgtat actaaaacct tccattcatt cgatggatga ttttcttttt gtgaaaaaat
     5701 attcattgta gtacgatgtt gcgcaacaca gacggatggt attgcttatt atttattaaa
     5761 atcgaacaat acacttattt aaaaacttct ttcccagatt ttcttccatt tttacagtta
     5821 taactagtat tatcatgaaa aatagccttt tattgtctat gggaataata atttaaaatt
     5881 ggtacttttt tattaattag ttcatcgccc aataatctct ccagtttctt agcatctcct
     5941 gactcaaccg gaattatcca agcccagtct aaataatttt ttaaaatctc atcatatttt
     6001 gcaaatattt cattgccata ttccgtcctt aaaattatat tttcagtgcc agacaagtga
     6061 tttatattga gttgataaaa aagatctcca tctaaaaaaa tatcattaga agaagatttc
     6121 cccacagtaa tgaccattcc atcagctcta tgtgttacat acaaataatt gtatgacctc
     6181 atttcaccta atgaatcaat tatttcttgc cctttagctc taatatattg atctctatta
     6241 cctatctcac tcttatttaa caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt
     6301 tttgtaaatt cgcttctact actaatccaa tccgagatta atttttcaac atgtcccaga
     6361 gtaaactctg atttattcgc taaatacttt ttttgttgta ttaaagacat ctttccccga
     6421 agctcaaaat tgagcctaca tcgattctct atctcttcaa aaagcatctt atcttcatca
     6481 ttcttatatt catcattcat aaaaccaatc cagctcctcg attaagataa tattttaatc
     6541 tcatacaaaa actagctctc atcaaaacaa ccattgtttt gatttctcat gaaatttctt
     6601 tatacttttc tccgattata aattcctaac cccttgagaa aattacgcat atatttatct
     6661 ccgcaaggct taaaatttcg ttggccttct tttctcaaaa atgccccgat ttcgcctttt
     6721 gatggataga tttctgcctc agcaaaaacg tctaaaatat catcagtggt catagacaag
     6781 gcgattttta atttttttag caacacattg ttgatatatt tgatttcctt gcttttttgc
     6841 ggcgtgtcta ctatcggctt atctttttgc ggaccacgcg ccaagagaat caggccattt
     6901 aggaatgcgt caaaaacttg gttgttgatg gttttttcat acacattatt ttctgcatta
     6961 tctttgttct tatcctgtac tttggtcagt accgcctgag cttcttcttt agtcagtggt
     7021 acaccgccta gctcaaaagc tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc
     7081 aatcgcaata acctatcatt gttattcatt tgttcctccg aagtatttag tctttacttt
     7141 cttacgtact ttttgtcttg ctaagatttt taggatcaat gataaaaacc gcatcactta
     7201 tgataaggcg ctgggtatca tgagtgaatg atattgctga atacaaatag tccgaccaca
     7261 ttaagttgat cggacttcat aagcatttta ttgactaata cttttttgct taagaattga
     7321 ttctaacatc ttttgctaaa gttaagcatc ctatagttcc tgcattatct gctaacttag
     7381 gtgtgacaat gtattcttcg atatttggcg tatcgacata gccgttaatt agttctaaaa
     7441 aacgtctttg tactttcttt ttcagatgtt cttgttgcat gacaccacca ccaaaaatta
     7501 taatatctgg tgaaagcatc agggtcgtat tacaggcaca ttgtgcaata taatccgcct
     7561 cgatttccca gaacggatct tctatagaaa gatcttgtcc cttggttccc gtacgcatct
     7621 cgatagcggg tccagatgcc aacccttcga gacaatcatg atgaaaagga caagtaccag
     7681 agaaagtatc tcctgaatgt ctcttaacta acgtatgccc catttctgga tggctaaacc
     7741 cttcaacaaa aatcccatct tgtatagctc ctccaccaat tccagttcca acagtgtaat
     7801 aaacaacact ggataaacct tttccatgac ctgaaacata ctccccgtaa caagcagcat
     7861 tcacatcggt tgtccaggca attggaattt cgaaatgctt attcaacgtt ccaacaaaat
     7921 caaaattttt ccaggcagtt tttggagtag aggtgatatg tccataggtc tttgattcac
     7981 gatggatatc aatcggtcca aatgacccta ctccaatgct ttctaattgt tctttatagt
     8041 gattaaagaa atcaattacc aaactcattg tttcattcgg tgtagttgta ggaaaactca
     8101 ctctttctaa aacctttaaa tcatcattgc caatggcaca aacaaattta gtcccacctg
     8161 cttctattga gccatacatt tcttactcca ccgtcctttt atctaggaat tcgttcttcc
     8221 ctgtctaata gtttttcgaa aaactcatgt ggttcctttt ggtaccaata ggcaacagaa
     8281 gaaatatcgt cttgacgctc ataaagcttg atgtcatcat ttcctagggt ttggaaagta
     8341 acacgtaaat tttctttgaa actaatgggg tcgggtaaat gccagcggta caacccatgc
     8401 atcggtaaag catcatcgcc aaaaccatga actggatttg gattaggttt tccagcacta
     8461 aaataatccc gggtgtgatc tcttttagat tgaaaaggat agcccataaa tagcgtatta
     8521 aaattttgag catatgccct ttctccaaag ttctttttat ggaaagccca agcgccgcca
     8581 aaataatctt ctgctcctgt agatgttatt gtagggaaat cttcatctcc atctatataa
     8641 aacttaaatt ctccttcccc ccaccagtat ctttccaatg cgcacaaagc aaagtaggtt
     8701 cctacataat acccagtgcc tttgattcca tcaataaggg tgtaatcttt tccttttgtc
     8761 gtgatgggtt cacggttcca tttagcatga aaatacatcg catcatccgg tacttcagtc
     8821 aatgcataat taaatgtata aaaaatgtgc ttaataaact caggatgttc attcgttaaa
     8881 gtaatcttag ctttttttcg aaatggcatc tcaaaatagc aattcatccc tgatgttgga
     8941 ttcactacga ttggtaagga attcacttcg catcttgtcc caaacccatt acaaaaaaaa
     9001 tctcctaatg gactttctac agaaggagtg gattcatcat cccaataaat acgcaaaatt
     9061 aaatcacgta agacgaaact cccagcgatt gttttgtctg tcactgtcat ccaaaaatga
     9121 cgtaaaattc cagggccttc catttctgct aaagtaattg tctctccagc atttatcgga
     9181 acagaaggtg acccttttct cccaattcct agatgactcg ctgtcattgc cgctttccct
     9241 ttttcaccat ttttattttc tggtgaaata gttctacttt cctctccctt gtggagccat
     9301 atattattta aacaattcat tttattcgtc ctctatcctt tcttcacttt tttgattatt
     9361 catatagatg atttgttgtt gtgcctccgc aataattgct ccaatatcta aattatcatt
     9421 tcctaactgc aaagcgattg acattgccaa gggaagattc attcctgcaa ttacgtgcac
     9481 tctgtcagaa atataggtaa aaaacttttg atttacactt cctccagcca tgtcagttaa
     9541 aacaattatt tcgtctgtag tatccactaa ctctttaatt tgttcatcta tatcatcaat
     9601 ttcttcgaca tatgcacata agctactaat ttgattatgc atacctgtta aaaactctag
     9661 tgtctcttta aaaccctcag ctagtattga atggctagca atgatcaatc ttctcataat
     9721 tcaaatcctt ttcctttacg ctaatattcc aaatgcagcc ataaccatcg caaaaacaat
     9781 aacgagtaga atcaaaatgg tcatactgat ttttttctta ctgagtagat aatatgctgc
     9841 agcagtaata attactggaa ttaatgttgg taaaattttg tccaacattg gttggatttt
     9901 catactaact tcaccaaaag tgaatttaag tggcgttgaa acactgataa ctgaagcaat
     9961 taatgatcca actactgtta gccccaatac tgaagcagct tcagttaaat aattaatctt
    10021 ttcaccgtat tcagtaacaa tcttcattcc ctgcttataa ccaaattcaa agcctctcat
    10081 tcggaaaaag aagattacca tgattaaaat gaaccataaa aagattccta caggattatt
    10141 ttctaaagcc atataagccg caattgatcc catgatggtt gggatcatcg tcatcaaaag
    10201 agagtcccca ataccagcaa ttggacccat cgtaccaact ttaaagtcat taatcgcatc
    10261 ttttgcttct aatccttgct tttcctccat tgcaacacaa gcacctaaaa taatggctgc
    10321 cagccaaggc tgtgtattgt aatactgaaa gtgattattt aaagcttcga tgtattcatc
    10381 atcatctttg tataattttc ttaaaatagg tgacaatgcg taagtcacag atgcaccttg
    10441 ctgtgtttga taattaaagg tacaaactga catcaaccaa cgatagttcg ctttcctaag
    10501 atcctttttc gttacttgat atttttcgct cattttattc atcctcccca ataaacccaa
    10561 catcagaagc tcccgcagaa gcagtgattt gtgcatcttt ttcagctcta tgatacacat
    10621 taaatgctaa agcgaatcct atgatcgccg ctccaataat aggtaccttc aaaaaagcgg
    10681 ctaagaaaaa tccaacaagt aaataactaa tgtatcgacc tgtaggcata aagcgtaaca
    10741 acatcgcaat accaacagca ggcaacattt ttccggctaa tgttagccca tccataaacc
    10801 aattaggtaa aacatccaaa acgtaattaa cagcagactg tcctaaagta acggcaataa
    10861 atactggaat tgctgcagat aaaccaaaga aaattggccc caaccacaaa acactattca
    10921 ttttcttaaa ttctcgttta ttcgcatatt cctgtgattt gcgaacaaca aaaccattta
    10981 agattttgac aattacatct agttgcaccc ctaacatacc tacaggtagt cctattgcta
    11041 atccggcttc tactccaagc tggctaccaa atgccgttgc aataattgcg cacgttccat
    11101 agtccggtac agatgagcca ccaaaaccgg caactcctaa tgacatcaat tgaactgtac
    11161 cgccaactat caaacctgtt tttatatcac ccatcaaaat tcctgtgaat aatccccaaa
    11221 aaactgcgta atttacaaag atcatcggaa tcccataata atcaacatgt ttaataaatg
    11281 ctaatatagt cagcataata atttgaaaaa tagtaaaatg cattatcctt actcctttat
    11341 cctaattttg attgaatttc ctgtgcctta tccgccggaa caaattgtgt aacaatttct
    11401 acaccagaat ccaacaattt tttatacgtg ctgatatttt catctgatgc ataggctcta
    11461 gaagatagct gtaatgcatt atctttaaca acagtaccgc caacaattag tcggtcaatt
    11521 ctaataccgt tatctactaa atgaaggatt gtttccggct ctttcacgat gacaaatact
    11581 ttttcctgat cgtatttccc ctttttaaaa ttcgcaatcg ctgtttcttc agtgataatt
    11641 gatacagcca ttccagcagg tctagctaaa cgcatacttg atttttggat gtcgttattt
    11701 gcgacagaat catcgatcac catcactctg tttgctttag acattggtgc ccattgcgtg
    11761 acaataatcc catgcaataa acggttatca actctcgaca taacaatact cataaatact
    11821 tcctccttta tctttcattt tcttacaagc aattaccatg ccaaaacaaa aagaacttga
    11881 taaatcaagc tctttttaca taatcataaa cgtatttaac ctcatgaatc gggatgtcga
    11941 cactataata atgttcaacg acactgaaag attgcttaaa atgtgtctga aatttttgaa
    12001 attcctcact cgtttcattg aaataaattt catcttccaa agtattttgt tttactaatc
    12061 gttcaattaa gcaactaatg tgaacatata atccgaaaca cgtattatta tcaaatgaaa
    12121 ttcctagatc attttgtaag atgtatatag catctgaaac ccgttctaat aatttagtcg
    12181 gatttaatat ggtcaattgc tccattaaat tcgttagact aaaattcttc agaatattat
    12241 tgttcaaaac tttcagttca ttatcattaa agtgtctatt caattga
//
LOCUS       DACDCV010000081        12002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDCV010000081 DACDCV010000000
VERSION     DACDCV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.081"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..512)
                     /gene="frr"
                     /locus_tag="IV041_002182"
     CDS             complement(<1..512)
                     /gene="frr"
                     /locus_tag="IV041_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8382938.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEV"
     gene            complement(515..1237)
                     /locus_tag="IV041_002183"
     CDS             complement(515..1237)
                     /locus_tag="IV041_002183"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8382939.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1373..2254)
                     /locus_tag="IV041_002184"
     CDS             complement(1373..2254)
                     /locus_tag="IV041_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8382940.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2353..3135)
                     /gene="rpsB"
                     /locus_tag="IV041_002185"
     CDS             complement(2353..3135)
                     /gene="rpsB"
                     /locus_tag="IV041_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8382941.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3494..3973
                     /locus_tag="IV041_002186"
     CDS             3494..3973
                     /locus_tag="IV041_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8382942.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4190..5281
                     /gene="phnW"
                     /locus_tag="IV041_002187"
     CDS             4190..5281
                     /gene="phnW"
                     /locus_tag="IV041_002187"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8382943.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5274..5402
                     /locus_tag="IV041_002188"
     CDS             5274..5402
                     /locus_tag="IV041_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8382944.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5406..5951
                     /locus_tag="IV041_002189"
     CDS             5406..5951
                     /locus_tag="IV041_002189"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8382945.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6405..8096)
                     /locus_tag="IV041_002190"
     CDS             complement(6405..8096)
                     /locus_tag="IV041_002190"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8382946.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8516..9463)
                     /gene="arcC"
                     /locus_tag="IV041_002191"
     CDS             complement(8516..9463)
                     /gene="arcC"
                     /locus_tag="IV041_002191"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8382947.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9578..10597)
                     /gene="argF"
                     /locus_tag="IV041_002192"
     CDS             complement(9578..10597)
                     /gene="argF"
                     /locus_tag="IV041_002192"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8382948.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10688..11917)
                     /gene="arcA"
                     /locus_tag="IV041_002193"
     CDS             complement(10688..11917)
                     /gene="arcA"
                     /locus_tag="IV041_002193"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8382949.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3273 a         2499 c         2184 g         4046 t
ORIGIN      
        1 acttctttga tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca
       61 tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg
      121 atgttacgaa tcgcgatttt tgcattttct gcttcttttt taacatcttt agctaattct
      181 tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt
      241 ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg
      301 tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc
      361 aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca
      421 cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt
      481 tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga
      541 tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca
      601 gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt
      661 tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg
      721 gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga
      781 tttctgccgc gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc
      841 ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg
      901 gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt
      961 ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag
     1021 cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccgc
     1081 ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt
     1141 ctttgattac tggtggtttg attccaaatc ctgcgtcccc agctaaagct tctccactca
     1201 attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta
     1261 ttgccatttt accatactta gatgatttcg acactctaag actggttttt cttgttttgc
     1321 ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt
     1381 tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa
     1441 cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag
     1501 tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct
     1561 tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt
     1621 gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta
     1681 cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag
     1741 ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa
     1801 atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga
     1861 taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca
     1921 ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta
     1981 aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg
     2041 ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt
     2101 tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc
     2161 cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta
     2221 gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct
     2281 ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc
     2341 catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc
     2401 gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg
     2461 gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc
     2521 atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat
     2581 agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg
     2641 gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt
     2701 ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa
     2761 agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt
     2821 atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg
     2881 agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac
     2941 gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt
     3001 tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt
     3061 tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat
     3121 agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc
     3181 taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc
     3241 tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat
     3301 atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac
     3361 agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa
     3421 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
     3481 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
     3541 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
     3601 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
     3661 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
     3721 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
     3781 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
     3841 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
     3901 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
     3961 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
     4021 tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
     4081 atttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
     4141 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
     4201 attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
     4261 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
     4321 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt
     4381 cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     4441 aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     4501 cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga
     4561 agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     4621 gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga
     4681 cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt
     4741 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     4801 taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga
     4861 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     4921 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     4981 atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt
     5041 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     5101 ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta
     5161 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     5221 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     5281 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     5341 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct
     5401 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     5461 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     5521 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     5581 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg
     5641 gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac
     5701 cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     5761 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     5821 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     5881 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     5941 agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt
     6001 aaacaattta aaagagttaa cagaatacat tttgtacgct gacatgaatc cagaatgact
     6061 tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa
     6121 gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     6181 tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     6241 ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     6301 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     6361 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     6421 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     6481 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     6541 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     6601 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     6661 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     6721 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     6781 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     6841 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     6901 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     6961 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     7021 tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     7081 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     7141 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     7201 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     7261 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     7321 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     7381 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct
     7441 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     7501 tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     7561 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     7621 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     7681 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     7741 atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct
     7801 ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     7861 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     7921 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     7981 acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt
     8041 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     8101 attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     8161 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     8221 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     8281 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     8341 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     8401 aaaaaaaaac gaaaggctag tcgtttcata aaaaaacaat ccagatggcc taaaggagaa
     8461 gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat
     8521 cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag
     8581 tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg
     8641 gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag
     8701 taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc
     8761 ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat
     8821 ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc
     8881 caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg
     8941 cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat
     9001 cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt
     9061 agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa
     9121 cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca
     9181 tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag
     9241 gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt
     9301 gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat
     9361 actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga
     9421 tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt
     9481 cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat
     9541 gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt
     9601 tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa
     9661 attgacgagc atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa
     9721 agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt
     9781 gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt
     9841 aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata
     9901 catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca
     9961 attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt
    10021 gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata
    10081 aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg
    10141 ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat
    10201 cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct accatttttt
    10261 gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt
    10321 ctgtggattc ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac
    10381 ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata
    10441 aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt
    10501 tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg
    10561 ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg
    10621 tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg
    10681 aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac
    10741 gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca
    10801 attcgttcga tacataattt cggttataag tgactacaac acctggagcg attgccaatg
    10861 tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg
    10921 ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg
    10981 aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt
    11041 ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg
    11101 tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc
    11161 ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt
    11221 gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat
    11281 gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat
    11341 gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat
    11401 tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca
    11461 ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac
    11521 gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt
    11581 ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt
    11641 catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg
    11701 ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt
    11761 catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa
    11821 tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt
    11881 tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg
    11941 attacatgta taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa
    12001 tg
//
LOCUS       DACDCV010000082        11670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDCV010000082 DACDCV010000000
VERSION     DACDCV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.082"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            478..972
                     /locus_tag="IV041_002194"
     CDS             478..972
                     /locus_tag="IV041_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382950.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1134..2306)
                     /locus_tag="IV041_002195"
     CDS             complement(1134..2306)
                     /locus_tag="IV041_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8382951.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2512..3327)
                     /locus_tag="IV041_002196"
     CDS             complement(2512..3327)
                     /locus_tag="IV041_002196"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8382952.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3477..3923)
                     /locus_tag="IV041_002197"
     CDS             complement(3477..3923)
                     /locus_tag="IV041_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8382953.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3920..4924)
                     /locus_tag="IV041_002198"
     CDS             complement(3920..4924)
                     /locus_tag="IV041_002198"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8382954.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4964..5293)
                     /locus_tag="IV041_002199"
     CDS             complement(4964..5293)
                     /locus_tag="IV041_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8382955.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5419..7746)
                     /locus_tag="IV041_002200"
     CDS             complement(5419..7746)
                     /locus_tag="IV041_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8382956.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(8589..8882)
                     /locus_tag="IV041_002201"
     CDS             complement(8589..8882)
                     /locus_tag="IV041_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8382957.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(9285..10463)
                     /locus_tag="IV041_002202"
     CDS             complement(9285..10463)
                     /locus_tag="IV041_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8382958.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10567..10881)
                     /locus_tag="IV041_002203"
     CDS             complement(10567..10881)
                     /locus_tag="IV041_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8382959.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(11201..11596)
                     /locus_tag="IV041_002204"
                     /pseudo
     CDS             complement(11201..11596)
                     /locus_tag="IV041_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3387 a         2274 c         2025 g         3984 t
ORIGIN      
        1 tctttttttc ctaagtattc tattgatatg aatcatttaa tcgtttatta tataaatgtc
       61 aataatctaa taataaaata acgtacatat tttattattg catacttaat aacagaacta
      121 tagcaaaaaa tcccgtgtat caacacggga ttagattaag tttcctcaat cactattcat
      181 aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg aaaacggttg
      241 ttttttactt aaggaggaaa tttagtgaaa aaattagttg cgttgttttt ggtcgcagga
      301 agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag ttcagctcag
      361 gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa agaacaaatt
      421 gaaaagaaaa aagaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaaagtg
      481 acagaagcgg aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca
      541 actgcttcag cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt
      601 gctgcaattg attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa
      661 gaaattgcac gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca
      721 gaacaggcga ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaagca
      781 gcagctgaac aggcttctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa
      841 actgtctatg tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg
      901 tatacccctg ccactttaag cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt
      961 tatggaaact aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa
     1021 tagaagctgg cgttccagat agtaaaccaa aaatagcagt ttacacgtcc atttcgtcag
     1081 cttctctctt ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct
     1141 ttctcaatca aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat
     1201 ttcattgaaa actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca
     1261 tgttgcaagc gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca
     1321 gaagctttca caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt
     1381 acagaaaaga ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa
     1441 ggatagtttc gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag
     1501 ccgattgatc caagtagtat accgataaac caaataaaaa atttccattt tttccattga
     1561 tcaattcctt ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc
     1621 acaagcagtc gatgtgtatt gaccatataa ggagtgcagg taagcaaagt cacgagatct
     1681 ctgccttttt ggatacccaa agaatctact tcgtcaggta agaccacttg gattttttct
     1741 acttgatacg ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac
     1801 ttaggcaaat ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca
     1861 tgggtgtttt tcccgccagt cggataagaa cttccttcca ataatgacgt tcctttttgc
     1921 aaaaatattt ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg
     1981 atgactccaa gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt
     2041 gcatcaactg cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt
     2101 tggtttttct ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat
     2161 gcttgctttt gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct
     2221 acaaaaggat aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc
     2281 agtattctta ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga
     2341 ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc
     2401 ctattttgcc gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc
     2461 ccatttgaat cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc
     2521 tgtttcttga tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac
     2581 caagatgaat acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt
     2641 atcgatcgtt gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc
     2701 agcttcattc ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc
     2761 cccttgtttc agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc
     2821 ttcccagtca gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag
     2881 gtttgctatt tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc
     2941 tgcatcggct aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta
     3001 gttttggaca ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac
     3061 accgtttgga ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg
     3121 caattttccg ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc
     3181 atctggtgct tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat
     3241 ggatccttca gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac
     3301 accagtgttt tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg
     3361 cgcccctgct aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct
     3421 ttctttgatg acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag
     3481 ggtaagctta acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag
     3541 acgtatacag catctttccc atcagattta cctggaagag tcaggttagc taatccttca
     3601 ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt
     3661 ggatagtacg tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta
     3721 ttcggaaatg tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc
     3781 aatttatgca aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct
     3841 gcagttccta ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat
     3901 atttttgttt tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt
     3961 tataaaacca agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc
     4021 caagcatctc ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat
     4081 ttgtctgcat ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga
     4141 tgatttccaa tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa
     4201 tggttgattg actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc
     4261 caaaagacaa tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc
     4321 ttgaatcatc tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat
     4381 tttttttgac tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga
     4441 caaattgttt gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg
     4501 tcaaacgtac atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt
     4561 aacggatcgt taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga
     4621 caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa
     4681 aatcgatcgt ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg
     4741 ttcctatctc gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc
     4801 catctgcaaa atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta
     4861 ttttttgttc gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg
     4921 tcacaggaag cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta
     4981 tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag
     5041 aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac
     5101 tatcactttt ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca
     5161 gcttgtaaaa ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta
     5221 ctccttgaaa gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat
     5281 cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag
     5341 tcggagaacc tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac
     5401 gtacattttt tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata
     5461 aaagaagcca gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc
     5521 acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat
     5581 aatcgtataa ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc
     5641 aatatctgtc cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc
     5701 ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc
     5761 gtcatcgaca gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc
     5821 tatatcagta aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt
     5881 tagtttaatt gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt
     5941 gtaatccata ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc
     6001 tagcttgaca tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag
     6061 ctctaccgca ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa
     6121 taaatagttt gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt
     6181 ataggttcca atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa
     6241 atcagttgga ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga
     6301 ttctgtttta tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta
     6361 cttactgcca tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc
     6421 agaaatctgt tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc
     6481 taaaacactc attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata
     6541 tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt
     6601 cattatttta gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg
     6661 tgtttgtttg cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc
     6721 gtttgtttgt gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc
     6781 cacatggatt ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac
     6841 tgtataagaa aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt
     6901 tggaaaattt gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc
     6961 cgtgaatgga atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc
     7021 ggcaggaagc cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt
     7081 aactgaatcg atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt
     7141 gatagtagta ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc
     7201 aataaaagcg attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc
     7261 gttaaccgca acaatgactc catctttggc atatatcact gtatatccat ctggtatggt
     7321 atgtccgttg aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc
     7381 tgaataagct ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat
     7441 cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc
     7501 tatggactgt atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa
     7561 atttgacgtt tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa
     7621 aatcaagatg atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag
     7681 tctagaaaca aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat
     7741 tttcatccct cctctcaata aaaaatcatc aaataatcct ttacacgtta ttctaaattt
     7801 aatttttatt attttattga tgtatagaaa gtttggggct ttttttctct ttttattcta
     7861 aggttatttt agtcttgtcc tttaatacaa agattttatt tttaaataaa aaatactctt
     7921 tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt gcttaaattt
     7981 ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt tttctttgtt
     8041 ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt gttgtgtccc
     8101 tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa aatggtaaag
     8161 tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata ctgttttcac
     8221 aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt tatcttttta
     8281 aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat tcgtacaaat
     8341 aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg tacgctcctg
     8401 gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct tttatcatag
     8461 ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg acaaaaaaag
     8521 ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat gagtataaat
     8581 taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga acttcttttg
     8641 aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact agaaaatacg
     8701 ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc aaagcgtttc
     8761 aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg ttttactttt
     8821 ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata agtataaacc
     8881 atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat tctggatttt
     8941 ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt ttactactac
     9001 ttataggtag atttattcta atccattatt ctcccctacc aataattaaa gaaaaaactg
     9061 gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca agggttcttt
     9121 ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa agtcgttaaa
     9181 gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt tgttaaaaaa
     9241 tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact ctctaaagaa
     9301 ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata aattttcgaa
     9361 gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca gtgttccgct
     9421 actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt aattcctgag
     9481 cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg aacaccacgt
     9541 ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct gtaatgttgc
     9601 ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag aggagttact
     9661 agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc cttactctta
     9721 cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc tatatctcca
     9781 ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac atggaaaagt
     9841 acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct caattcatcg
     9901 acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt taccgttaac
     9961 tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt ctttctttga
    10021 caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt tttggcatag
    10081 ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc ttgaaggcac
    10141 tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat ttttaattgt
    10201 tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta atattggaac
    10261 cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt cttaagcctt
    10321 tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt aaagccacgt
    10381 cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact gatttgcttc
    10441 ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa cccaacaagc
    10501 agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt agaaagaaaa
    10561 tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg aatcactctt
    10621 tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt accaacaggg
    10681 aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct gaaatagtcg
    10741 ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca atttttccgt
    10801 gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag aattaacatt
    10861 tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc accacggtac
    10921 cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga aaagaggaat
    10981 ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc
    11041 tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa aaaaacttaa
    11101 aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa tcacagaatt
    11161 tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tcctagctct tccaaagcca
    11221 tttgtcgttg gtaccaaaca ctgccgagac ttttcccaat aatatcgcca atttcacgat
    11281 cattaagacc gatgaagaag gacataaaaa tgatttcttg tctttgacta ggtaatgctg
    11341 caatcgcatt tgccaactcc tcattggaaa taaagatttt cataccaagg acaataaaag
    11401 taccactact attcaaattt ggttccaaat aatagaactc tgtacctaac cctttttgtt
    11461 ccaatatttc cattgatatt tgccgttggc gaaatctagc gtacttcttt tgaatattta
    11521 acgcctcatt gcagactact ttcttacaaa aactgtcaaa cgtatgttga atgtgggttt
    11581 cctctgagtc gcccataata ttctccccct caaaaaattc attcaactcg ttttacttct
    11641 atccctttcc attattacta caatctagga
//
LOCUS       DACDCV010000083        11669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDCV010000083 DACDCV010000000
VERSION     DACDCV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.083"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..634)
                     /locus_tag="IV041_002205"
     CDS             complement(53..634)
                     /locus_tag="IV041_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8382960.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(640..1899)
                     /locus_tag="IV041_002206"
     CDS             complement(640..1899)
                     /locus_tag="IV041_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8382961.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1901..2947)
                     /locus_tag="IV041_002207"
     CDS             complement(1901..2947)
                     /locus_tag="IV041_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382962.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3182..3457)
                     /locus_tag="IV041_002208"
     CDS             complement(3182..3457)
                     /locus_tag="IV041_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8382963.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3700..5010)
                     /locus_tag="IV041_002209"
     CDS             complement(3700..5010)
                     /locus_tag="IV041_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8382964.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5192..6421)
                     /gene="rpsA"
                     /locus_tag="IV041_002210"
     CDS             complement(5192..6421)
                     /gene="rpsA"
                     /locus_tag="IV041_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8382965.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6556..7236)
                     /locus_tag="IV041_002211"
     CDS             complement(6556..7236)
                     /locus_tag="IV041_002211"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8382966.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7285..7920)
                     /locus_tag="IV041_002212"
     CDS             complement(7285..7920)
                     /locus_tag="IV041_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8382967.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7986..9389)
                     /locus_tag="IV041_002213"
     CDS             complement(7986..9389)
                     /locus_tag="IV041_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8382968.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(9376..10407)
                     /locus_tag="IV041_002214"
     CDS             complement(9376..10407)
                     /locus_tag="IV041_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382969.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10438..10659
                     /locus_tag="IV041_002215"
     CDS             10438..10659
                     /locus_tag="IV041_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8382970.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10824..11561)
                     /locus_tag="IV041_002216"
     CDS             complement(10824..11561)
                     /locus_tag="IV041_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8382971.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT         3253 a         2487 c         1953 g         3976 t
ORIGIN      
        1 agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt
       61 tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt
      121 catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag
      181 cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt
      241 cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag
      301 atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt
      361 tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca
      421 aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg
      481 caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat
      541 aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta
      601 aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc
      661 taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc
      721 ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc
      781 tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc
      841 ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc
      901 catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact
      961 taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc
     1021 ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc
     1081 gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat
     1141 ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat gcggattaac
     1201 ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt
     1261 aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg
     1321 tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat
     1381 cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc
     1441 ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc
     1501 atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat
     1561 aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc
     1621 cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc
     1681 taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg
     1741 ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc
     1801 tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc
     1861 tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac
     1921 tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt
     1981 atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa
     2041 aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc
     2101 caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga
     2161 gattccacac gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat
     2221 ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt
     2281 ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg
     2341 ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa
     2401 tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc
     2461 tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat
     2521 ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata
     2581 cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat
     2641 ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt
     2701 tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa
     2761 tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat
     2821 cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct
     2881 gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa
     2941 gttccatacg catccctcct cttcttattt ttattttacc attagtaagc aaattctaga
     3001 aaagataaga gcaaagctca cctcataact tggaaataaa taaaaattaa ataaaataca
     3061 tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct
     3121 gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt
     3181 cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg
     3241 gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa
     3301 cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa
     3361 tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg
     3421 aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa
     3481 gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag
     3541 aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa
     3601 ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc
     3661 tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat
     3721 aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg
     3781 ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt
     3841 gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg
     3901 attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt
     3961 ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac
     4021 tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg
     4081 ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat
     4141 aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat
     4201 cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc
     4261 acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc
     4321 tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc
     4381 tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat
     4441 caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc
     4501 ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc
     4561 tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata
     4621 gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt
     4681 acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct
     4741 gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg
     4801 atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg
     4861 acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac
     4921 aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg
     4981 gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc
     5041 acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat
     5101 ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct
     5161 gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt
     5221 cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt
     5281 cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt
     5341 ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca
     5401 ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa
     5461 tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc
     5521 ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt
     5581 ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat
     5641 tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca cttggttttg
     5701 ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga
     5761 caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga
     5821 tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg
     5881 ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc
     5941 ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac
     6001 cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga
     6061 tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac
     6121 ggcgttttga aagtaggtag ctgccgtttt ctttatcttt gccgatcgta ctgataacga
     6181 ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg
     6241 ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt
     6301 gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta
     6361 cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca
     6421 taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt
     6481 gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc
     6541 attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc
     6601 tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg
     6661 cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt
     6721 cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc
     6781 acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt
     6841 cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc
     6901 aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc
     6961 tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc
     7021 ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga
     7081 aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc
     7141 tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga
     7201 agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa
     7261 aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat
     7321 aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg
     7381 ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc
     7441 tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc
     7501 ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt
     7561 atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt
     7621 tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga
     7681 agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg
     7741 aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt
     7801 cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg
     7861 ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac
     7921 tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta
     7981 aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgtttc ccaatgaagt
     8041 tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata
     8101 tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt
     8161 ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct
     8221 ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc
     8281 tccatccatt ttttttgcag ctcatcaaag ggagcttgct ttgtggcata ttccaaataa
     8341 agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca
     8401 tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg
     8461 atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata
     8521 tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt
     8581 tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat
     8641 ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca
     8701 caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt
     8761 tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct
     8821 tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc
     8881 gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga
     8941 tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat
     9001 aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga
     9061 ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc
     9121 gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact
     9181 tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac
     9241 cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct
     9301 tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca
     9361 aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat
     9421 ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg
     9481 ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat
     9541 ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt
     9601 tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat
     9661 ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc
     9721 gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct
     9781 ttgttgcaga taaacctctg actcatcatt gttttttgcc agctgataag gaagcagccc
     9841 tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc
     9901 cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg
     9961 gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag
    10021 atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg
    10081 ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg
    10141 atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc
    10201 gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc
    10261 tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt
    10321 cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga
    10381 aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg
    10441 tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa
    10501 gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag
    10561 gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa
    10621 tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa
    10681 cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa
    10741 atcccgactt attttgaaag attcatttgc tatctatagt taattgacta gttttttcac
    10801 ggaccctata ggacccattt atattatttc aactttttca gccagttttc caatgtattg
    10861 acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca
    10921 ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc
    10981 ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc
    11041 gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt
    11101 gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt
    11161 gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct
    11221 ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata
    11281 gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct
    11341 acgagagcac caggataaaa aataagtaat ggagactttc catgattctt tttctctcct
    11401 tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac
    11461 gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa
    11521 atgatgacta agccaattag tattcgtcta aatattttca ctttctccca ccttttctat
    11581 ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat
    11641 gcaaactttc cagcatcaaa taaaaaaag
//
LOCUS       DACDCV010000084        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDCV010000084 DACDCV010000000
VERSION     DACDCV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.084"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IV041_002217"
     CDS             complement(37..216)
                     /locus_tag="IV041_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382972.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IV041_002218"
     CDS             complement(597..1304)
                     /locus_tag="IV041_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382973.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IV041_002219"
     CDS             complement(1297..1734)
                     /locus_tag="IV041_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382974.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IV041_002220"
     CDS             complement(1749..2000)
                     /locus_tag="IV041_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382975.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IV041_002221"
     CDS             complement(2000..2206)
                     /locus_tag="IV041_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8382976.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IV041_002222"
     CDS             complement(2643..2903)
                     /locus_tag="IV041_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382977.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IV041_002223"
     CDS             2978..3181
                     /locus_tag="IV041_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8382978.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV041_002224"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV041_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8382979.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IV041_002225"
     CDS             complement(3810..4709)
                     /locus_tag="IV041_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8382980.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IV041_002226"
     CDS             complement(4722..5315)
                     /locus_tag="IV041_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8382981.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV041_002227"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV041_002227"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8382982.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IV041_002228"
     CDS             complement(7771..9231)
                     /locus_tag="IV041_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8382983.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IV041_002229"
     CDS             complement(9292..11664)
                     /locus_tag="IV041_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382984.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DACDCV010000085        11624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDCV010000085 DACDCV010000000
VERSION     DACDCV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.085"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(119..1249)
                     /locus_tag="IV041_002230"
     CDS             complement(119..1249)
                     /locus_tag="IV041_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8382985.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1790..1996)
                     /locus_tag="IV041_002231"
     CDS             complement(1790..1996)
                     /locus_tag="IV041_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382986.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="IV041_002232"
     CDS             complement(2012..2626)
                     /locus_tag="IV041_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8382987.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="IV041_002233"
     CDS             complement(2773..3456)
                     /locus_tag="IV041_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8382988.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="IV041_002234"
     CDS             complement(3565..4023)
                     /locus_tag="IV041_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8382989.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="IV041_002235"
     CDS             complement(4107..5870)
                     /locus_tag="IV041_002235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8382990.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="IV041_002236"
     CDS             complement(6091..7551)
                     /locus_tag="IV041_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8382991.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IV041_002237"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IV041_002237"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8382992.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="IV041_002238"
     CDS             complement(8708..10135)
                     /locus_tag="IV041_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8382993.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="IV041_002239"
     CDS             complement(10166..11380)
                     /locus_tag="IV041_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8382994.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3522 a         2404 c         1916 g         3782 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11461 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11521 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    11581 tcttttttta tttttaaaga tattttttca taaggaataa agaa
//
LOCUS       DACDCV010000086        11357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDCV010000086 DACDCV010000000
VERSION     DACDCV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.086"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..325)
                     /locus_tag="IV041_002240"
     CDS             complement(<1..325)
                     /locus_tag="IV041_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8382995.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSE"
     gene            502..1230
                     /gene="sfsA"
                     /locus_tag="IV041_002241"
     CDS             502..1230
                     /gene="sfsA"
                     /locus_tag="IV041_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8382996.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1246..2259
                     /locus_tag="IV041_002242"
     CDS             1246..2259
                     /locus_tag="IV041_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8382997.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2259..3536
                     /locus_tag="IV041_002243"
     CDS             2259..3536
                     /locus_tag="IV041_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8382998.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3599..6301
                     /locus_tag="IV041_002244"
     CDS             3599..6301
                     /locus_tag="IV041_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8382999.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6453..6770
                     /locus_tag="IV041_002245"
     CDS             6453..6770
                     /locus_tag="IV041_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383000.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6800..7120
                     /locus_tag="IV041_002246"
     CDS             6800..7120
                     /locus_tag="IV041_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8383001.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7228..8688
                     /locus_tag="IV041_002247"
     CDS             7228..8688
                     /locus_tag="IV041_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8383002.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8756..8977
                     /locus_tag="IV041_002248"
     CDS             8756..8977
                     /locus_tag="IV041_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383003.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9008..9190
                     /locus_tag="IV041_002249"
     CDS             9008..9190
                     /locus_tag="IV041_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8383004.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9190..9603
                     /locus_tag="IV041_002250"
     CDS             9190..9603
                     /locus_tag="IV041_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8383005.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9726..10907
                     /locus_tag="IV041_002251"
     CDS             9726..10907
                     /locus_tag="IV041_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8383006.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT         3736 a         1920 c         2405 g         3296 t
ORIGIN      
        1 tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt tgttcgggat
       61 aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat tcatctattt
      121 ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca gatgtaatct
      181 gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac tttgagagtc
      241 cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag agaacggtaa
      301 tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt tatgagaaaa
      361 taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa gctaaaattt
      421 ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata ggatacatag
      481 taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt ttttattgac
      541 cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat tattgcgcat
      601 gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt ggctattgta
      661 tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa aaagaagggt
      721 gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg tattttagac
      781 ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg tgaagtaact
      841 tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa agcatttgtt
      901 gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga tgcgcccaca
      961 ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg ttatgccgct
     1021 tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca tgaagaaatg
     1081 caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca aatcctcgcc
     1141 tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc atttgatgta
     1201 gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat caacttaggt
     1261 attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag cacaaaaaaa
     1321 tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt tggttctcat
     1381 tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat tactcatatg
     1441 gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa gcaagcaatt
     1501 ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa agaaatggat
     1561 gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc aagaaatatc
     1621 catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga gataatcggt
     1681 gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga tggagaagaa
     1741 ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg tgtttacctt
     1801 gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt cccaagaaag
     1861 atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa ttttgatgta
     1921 tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta ttttgactca
     1981 ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca tgatcgcgcg
     2041 cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc agatgaagca
     2101 gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt gttgtatgaa
     2161 gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag aaaaaatggc
     2221 ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt gtacaagtat
     2281 ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct tctcttattc
     2341 aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt tctcctacag
     2401 gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa ccaggtgaag
     2461 caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta gcagatgttg
     2521 cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc ggagccaacg
     2581 tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt acaccaggaa
     2641 gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa acaatggtat
     2701 ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat caaattcttc
     2761 atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat cgttcccttg
     2821 aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca gcagaggacg
     2881 attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag aaagatgact
     2941 acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt aatcaactga
     3001 ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta ggaagtctgg
     3061 cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa aagaaaatgg
     3121 tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca ggtcttcctt
     3181 atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt tcgggaagaa
     3241 ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat aatctaagag
     3301 atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt ttatacggag
     3361 gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta aataaaaagt
     3421 atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa aaaataagaa
     3481 aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc aaataagcgc
     3541 ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga ggggttatgt
     3601 gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag gattgacagc
     3661 aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat atttaagtga
     3721 actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt actctttgcc
     3781 aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag tgacttttga
     3841 aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa aagctgcgat
     3901 tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta cagggaagtc
     3961 gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt ctgcagatgc
     4021 gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc tctttggaca
     4081 catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc ccggtctaat
     4141 agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac cgccggaagg
     4201 gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg gagaaagtgc
     4261 acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt cagaaagttt
     4321 cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc ttgcttcacg
     4381 ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag cgaatcgttt
     4441 gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt atgatgcgga
     4501 aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat cttttttaca
     4561 ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt cccttcaagt
     4621 acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat tcatggacag
     4681 caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt cacaagatcc
     4741 agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc tttccgaaac
     4801 cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg cttatttcca
     4861 gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc cagatgaaat
     4921 ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc gtatttataa
     4981 tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc gtgtgagaga
     5041 aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta agagggaatt
     5101 ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg agattccttt
     5161 tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa gtgagcctgt
     5221 taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg cttcaagcat
     5281 gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta atatgccact
     5341 ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa actatcaaga
     5401 gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca ccttcagtga
     5461 tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa gtaccatgat
     5521 tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata tttatcaaaa
     5581 tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa agtctgaaac
     5641 tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag tagctgccaa
     5701 attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga tccagatgca
     5761 gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag aatatgagat
     5821 caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt tttcagccta
     5881 tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg atgagatccc
     5941 gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt ctattgatga
     6001 actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt cgattatcgt
     6061 ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag acggattgat
     6121 caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa tttatcgttt
     6181 ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc aggtaaaaat
     6241 tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa taaaaaaatg
     6301 aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata aaaaagagct
     6361 aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct tgcgttatat
     6421 aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc atgttagttt
     6481 gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca gcagaagaaa
     6541 aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat aatttagaat
     6601 ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa gctcaatttg
     6661 aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa gactatggca
     6721 tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa atcaagtatg
     6781 cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca tggggttaat
     6841 catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg ctaaaatcgg
     6901 tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta tacaagcgca
     6961 tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc aagtgacatt
     7021 attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg atttagcaaa
     7081 agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc ttttttgaac
     7141 ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac tctctttata
     7201 taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat ggagaaatac
     7261 atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt gcgtgatgct
     7321 tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat caatgcaatt
     7381 attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc agcagacaaa
     7441 ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg gaacggtaca
     7501 ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc ccgagcttat
     7561 ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca aggtatttcc
     7621 tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt cgcagatgca
     7681 atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt tggattatgg
     7741 ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat gatcatggga
     7801 gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat caaaatgcct
     7861 gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc gacgatcgct
     7921 ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg gcaactgatc
     7981 attgatttgg tgcaaaaata tattgcagag ccattcctag gattatctca agggttggga
     8041 gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat tcatggacca
     8101 aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat caatattgat
     8161 attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga tgcaatcgaa
     8221 gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg gtttggcgga
     8281 tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg tgcagattat
     8341 ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga accagtgatg
     8401 tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat tgctccagta
     8461 gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc ggtatcgcag
     8521 caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg agcggattgg
     8581 cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg ggcaccattt
     8641 gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga tgaacgtgag
     8701 gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa ctgaaatgat
     8761 tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag gatacaatga
     8821 agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct ttgaaacaag
     8881 tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag cacagccttg
     8941 gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa aagataggat
     9001 ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga tcgtgttgta
     9061 tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag gtctttttct
     9121 aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag caaaggagaa
     9181 aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt ttacgataaa
     9241 gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt aactagaaaa
     9301 cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc tcgcataaaa
     9361 atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac gaagaatgaa
     9421 ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt tcattacaca
     9481 gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt aggaacaatc
     9541 ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga agcaacgtat
     9601 taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct agaaggaatt
     9661 tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta ggaaggggca
     9721 gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa atgtttgatt
     9781 tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt tttcgtacac
     9841 ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta acaagtcaag
     9901 tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg gccggcattg
     9961 gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag atcatggaaa
    10021 agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg ccattctcct
    10081 atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt tatcaactaa
    10141 acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt atggattttg
    10201 aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac caacgagctg
    10261 gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc aattaccagt
    10321 ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat cagcttgcaa
    10381 cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt tgcagccttg
    10441 ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca tgcggctacg
    10501 gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg atcacacctt
    10561 atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca tttaaaaaga
    10621 gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg aatgaaggaa
    10681 tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa caaacaaaac
    10741 tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg tatcgtaaag
    10801 tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa gaaacgttaa
    10861 gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa aaaacgggtg
    10921 atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag gaaaatgtta
    10981 aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt tagggttttc
    11041 ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt tagatgaatc
    11101 taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg acctccctac
    11161 attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac gttttgccta
    11221 acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac aagctacaat
    11281 tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc ctaagcgata
    11341 ggtcgctggt tcaattc
//
LOCUS       DACDCV010000087        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDCV010000087 DACDCV010000000
VERSION     DACDCV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.087"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV041_002252"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV041_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8383007.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV041_002253"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV041_002253"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8383008.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IV041_002254"
     CDS             complement(2169..2906)
                     /locus_tag="IV041_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8383009.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IV041_002255"
     CDS             complement(2997..3524)
                     /locus_tag="IV041_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8383010.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IV041_002256"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IV041_002256"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8383011.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IV041_002257"
     CDS             complement(4401..4886)
                     /locus_tag="IV041_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8383012.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IV041_002258"
     CDS             complement(5039..5236)
                     /locus_tag="IV041_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383013.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV041_002259"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV041_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8383014.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV041_002260"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV041_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8383015.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IV041_002261"
     CDS             9211..9423
                     /locus_tag="IV041_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8383016.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IV041_002262"
     CDS             9425..11104
                     /locus_tag="IV041_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8383017.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACDCV010000088        11174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDCV010000088 DACDCV010000000
VERSION     DACDCV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.088"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV041_002263"
     CDS             6..305
                     /locus_tag="IV041_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383018.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV041_002264"
     CDS             378..1739
                     /locus_tag="IV041_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8383019.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV041_002265"
     CDS             1761..2021
                     /locus_tag="IV041_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383020.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV041_002266"
     CDS             2035..2787
                     /locus_tag="IV041_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8383021.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV041_002267"
     CDS             complement(2881..3486)
                     /locus_tag="IV041_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8383022.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV041_002268"
     CDS             complement(3539..4513)
                     /locus_tag="IV041_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8383023.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV041_002269"
     CDS             complement(4891..5670)
                     /locus_tag="IV041_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8383024.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV041_002270"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV041_002270"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8383025.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV041_002271"
     CDS             7161..8558
                     /locus_tag="IV041_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8383026.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV041_002272"
     CDS             8639..9079
                     /locus_tag="IV041_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8383027.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV041_002273"
     CDS             9227..9553
                     /locus_tag="IV041_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8383028.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV041_002274"
     CDS             9554..10303
                     /locus_tag="IV041_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383029.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV041_002275"
     CDS             complement(10383..10838)
                     /locus_tag="IV041_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8383030.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3549 a         1956 c         2294 g         3375 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atag
//
LOCUS       DACDCV010000089        10591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDCV010000089 DACDCV010000000
VERSION     DACDCV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.089"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            216..485
                     /gene="rpsN"
                     /locus_tag="IV041_002276"
     CDS             216..485
                     /gene="rpsN"
                     /locus_tag="IV041_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8383031.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            500..679
                     /gene="rpmF"
                     /locus_tag="IV041_002277"
     CDS             500..679
                     /gene="rpmF"
                     /locus_tag="IV041_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8383032.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            711..1088
                     /locus_tag="IV041_002278"
     CDS             711..1088
                     /locus_tag="IV041_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383033.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1109..1258
                     /gene="rpmG"
                     /locus_tag="IV041_002279"
     CDS             1109..1258
                     /gene="rpmG"
                     /locus_tag="IV041_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8383034.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1282..2265
                     /locus_tag="IV041_002280"
     CDS             1282..2265
                     /locus_tag="IV041_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8383035.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2295..2417
                     /locus_tag="IV041_002281"
     CDS             2295..2417
                     /locus_tag="IV041_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8383036.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2435..3958
                     /locus_tag="IV041_002282"
     CDS             2435..3958
                     /locus_tag="IV041_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8383037.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3982..4221
                     /locus_tag="IV041_002283"
     CDS             3982..4221
                     /locus_tag="IV041_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383038.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4414..4875)
                     /locus_tag="IV041_002284"
     CDS             complement(4414..4875)
                     /locus_tag="IV041_002284"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383039.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5070..5288)
                     /locus_tag="IV041_002285"
     CDS             complement(5070..5288)
                     /locus_tag="IV041_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383040.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5406..7022
                     /locus_tag="IV041_002286"
     CDS             5406..7022
                     /locus_tag="IV041_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8383041.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7263..8603)
                     /locus_tag="IV041_002287"
                     /pseudo
     CDS             complement(7263..8603)
                     /locus_tag="IV041_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8879..9034)
                     /locus_tag="IV041_002288"
     CDS             complement(8879..9034)
                     /locus_tag="IV041_002288"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8383042.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9330..9662)
                     /locus_tag="IV041_002289"
     CDS             complement(9330..9662)
                     /locus_tag="IV041_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383043.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10176..10367)
                     /locus_tag="IV041_002290"
     CDS             complement(10176..10367)
                     /locus_tag="IV041_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383044.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10461..>10591)
                     /locus_tag="IV041_002291"
     CDS             complement(10461..>10591)
                     /locus_tag="IV041_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8383045.1"
                     /translation="FLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3515 a         1688 c         2060 g         3328 t
ORIGIN      
        1 tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga
       61 taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt
      121 gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag
      181 gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta
      241 aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag
      301 ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga
      361 ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc
      421 gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt
      481 ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg
      541 gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg
      601 tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat
      661 taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta
      721 tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc
      781 tagataaatg cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt
      841 taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct
      901 tatcatttga tgatttttta agaatctatc caatttttga agagtatgac gaggagttat
      961 ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac
     1021 aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag
     1081 atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga
     1141 aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga
     1201 attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct
     1261 aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg
     1321 gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa
     1381 attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat
     1441 agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat
     1501 ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca
     1561 caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca aacactttta
     1621 acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt
     1681 gacagttttg aacttattga aagagaacca caagccctta agcaacttgt catggcagat
     1741 cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga
     1801 gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt
     1861 caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat
     1921 tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt
     1981 aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt
     2041 gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta
     2101 caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac
     2161 accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa
     2221 aggatcaaac agagttttga taaattactg gcggaaagca aataatgata agtagtgatt
     2281 gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc
     2341 cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa
     2401 tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat
     2461 tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc
     2521 gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac
     2581 caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc
     2641 tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt
     2701 atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc
     2761 tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga
     2821 agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc
     2881 acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc
     2941 gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt
     3001 ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca
     3061 cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact
     3121 aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa
     3181 aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt
     3241 ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga
     3301 acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga
     3361 aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa
     3421 gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta
     3481 tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt
     3541 cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac
     3601 tactggctat aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt
     3661 agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta
     3721 tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg
     3781 ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca
     3841 tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac
     3901 tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa
     3961 ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat
     4021 attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta
     4081 gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg
     4141 gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg
     4201 cataatagct tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga
     4261 tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga
     4321 agacttcata aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa
     4381 tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt
     4441 ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg
     4501 aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt
     4561 tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat
     4621 cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa
     4681 gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat
     4741 atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat
     4801 cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt
     4861 tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc
     4921 gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg
     4981 ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg
     5041 acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct
     5101 tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc
     5161 agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa
     5221 aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac
     5281 tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc
     5341 gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt
     5401 tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga
     5461 tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg
     5521 acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg
     5581 gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga
     5641 ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt
     5701 caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag
     5761 acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac
     5821 gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg
     5881 tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca
     5941 tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc
     6001 taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact
     6061 tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct
     6121 ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta
     6181 tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta
     6241 cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac
     6301 atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa
     6361 attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg
     6421 aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg
     6481 gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc
     6541 ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta
     6601 gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa
     6661 ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg
     6721 agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa
     6781 tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg
     6841 gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc
     6901 ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt
     6961 taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact
     7021 aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca
     7081 ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc
     7141 acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt
     7201 tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac
     7261 ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta
     7321 ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
     7381 aagtttctat agccataggc aattcgtttt agtactttga tatggttatt caaacattct
     7441 atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg
     7501 aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc
     7561 gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg
     7621 tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc
     7681 agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc
     7741 ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
     7801 gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga
     7861 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
     7921 gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag
     7981 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
     8041 ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca
     8101 tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg
     8161 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata
     8221 gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag
     8281 cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     8341 cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa taagatggat
     8401 acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat
     8461 tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc
     8521 tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct
     8581 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     8641 ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     8701 cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc
     8761 aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat
     8821 cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg attaattctc
     8881 atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc
     8941 tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct
     9001 ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac
     9061 ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg
     9121 ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat
     9181 ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac
     9241 catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt
     9301 gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc
     9361 atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac
     9421 tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca
     9481 ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc
     9541 catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt ggtagttact
     9601 tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc
     9661 aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca
     9721 acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa
     9781 ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat
     9841 aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct
     9901 cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa
     9961 agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa
    10021 acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa cgctgtaaaa
    10081 tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa tttgaaacta
    10141 tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc gaatttcgag
    10201 atagctagat atattgcaag catacattct tgataacaat cttgatcata ttttccagtt
    10261 ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc aacagcttct
    10321 tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa tctaccttct
    10381 tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag aagcatacaa
    10441 aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta aagtcgatgt
    10501 tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc tttatcagtt
    10561 ttatcaaaga taaatttttc gataagaaaa a
//
LOCUS       DACDCV010000090        10536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDCV010000090 DACDCV010000000
VERSION     DACDCV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.090"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IV041_002292"
     CDS             23..646
                     /locus_tag="IV041_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383046.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IV041_002293"
     CDS             1096..2145
                     /locus_tag="IV041_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8383047.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IV041_002294"
     CDS             2142..3023
                     /locus_tag="IV041_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8383048.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IV041_002295"
     CDS             3026..3796
                     /locus_tag="IV041_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8383049.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IV041_002296"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IV041_002296"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8383050.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IV041_002297"
     CDS             5107..6270
                     /locus_tag="IV041_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8383051.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IV041_002298"
     CDS             6387..6797
                     /locus_tag="IV041_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8383052.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IV041_002299"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IV041_002299"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8383053.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IV041_002300"
     CDS             9337..9654
                     /locus_tag="IV041_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383054.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IV041_002301"
     CDS             9814..10050
                     /locus_tag="IV041_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8383055.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IV041_002302"
     CDS             10047..10445
                     /locus_tag="IV041_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8383056.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3668 a         1769 c         2230 g         2869 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag tttttt
//
LOCUS       DACDCV010000091        10520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDCV010000091 DACDCV010000000
VERSION     DACDCV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.091"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV041_002303"
     CDS             complement(25..144)
                     /locus_tag="IV041_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8383057.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV041_002304"
     CDS             complement(159..1343)
                     /locus_tag="IV041_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8383058.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV041_002305"
     CDS             complement(1599..1853)
                     /locus_tag="IV041_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383059.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV041_002306"
     CDS             complement(1878..3020)
                     /locus_tag="IV041_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8383060.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV041_002307"
     CDS             complement(3080..4432)
                     /locus_tag="IV041_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8383061.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV041_002308"
     CDS             complement(4503..4847)
                     /locus_tag="IV041_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8383062.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV041_002309"
     CDS             complement(4857..5231)
                     /locus_tag="IV041_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8383063.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV041_002310"
     CDS             complement(5244..5558)
                     /locus_tag="IV041_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8383064.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV041_002311"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV041_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IV041_002312"
     CDS             complement(8976..9638)
                     /locus_tag="IV041_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383065.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IV041_002313"
     CDS             10255..10485
                     /locus_tag="IV041_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383066.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2891 a         2341 c         1785 g         3503 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt
//
LOCUS       DACDCV010000092        10416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDCV010000092 DACDCV010000000
VERSION     DACDCV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.092"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(246..758)
                     /locus_tag="IV041_002314"
     CDS             complement(246..758)
                     /locus_tag="IV041_002314"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8383067.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(772..3069)
                     /gene="recJ"
                     /locus_tag="IV041_002315"
     CDS             complement(772..3069)
                     /gene="recJ"
                     /locus_tag="IV041_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8383068.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3211..3645)
                     /locus_tag="IV041_002316"
     CDS             complement(3211..3645)
                     /locus_tag="IV041_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8383069.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3655..4443)
                     /locus_tag="IV041_002317"
     CDS             complement(3655..4443)
                     /locus_tag="IV041_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8383070.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4440..5381)
                     /locus_tag="IV041_002318"
     CDS             complement(4440..5381)
                     /locus_tag="IV041_002318"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8383071.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5558..6145)
                     /locus_tag="IV041_002319"
     CDS             complement(5558..6145)
                     /locus_tag="IV041_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8383072.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6366..7679)
                     /gene="obgE"
                     /locus_tag="IV041_002320"
     CDS             complement(6366..7679)
                     /gene="obgE"
                     /locus_tag="IV041_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8383073.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7967..8977
                     /gene="gap"
                     /locus_tag="IV041_002321"
     CDS             7967..8977
                     /gene="gap"
                     /locus_tag="IV041_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8383074.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9062..9997)
                     /locus_tag="IV041_002322"
     CDS             complement(9062..9997)
                     /locus_tag="IV041_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8383075.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10183..>10416
                     /locus_tag="IV041_002323"
     CDS             10183..>10416
                     /locus_tag="IV041_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383076.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVD"
BASE COUNT         2915 a         2351 c         1803 g         3347 t
ORIGIN      
        1 tttacggggg tgtgacaaaa atcggtcaca tccttttttt cgaataaacg gtgttctaaa
       61 gccagttttt cggtattaag tcaaattgcg gaaaaagtga ttttttaaac ttctactggt
      121 ctttgcttgt ttccatttat aaaaaaatag cgaccctcaa aagtcagata gagaatctga
      181 cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata gcttcttttt
      241 tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc gcgtccatgg
      301 agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa ttgttcgatc
      361 agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca aatcagcaca
      421 cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc atattctaat
      481 ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac tggggcaaaa
      541 ccgactccta actcataagc aacaggacat cctacgatga atccacgggc ttctggccca
      601 actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt agcttcccga
      661 taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc ttttgacgga
      721 tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct cctaggttgt
      781 taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa ctttgatttt
      841 attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg ggctagcgac
      901 tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat
      961 aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta ttttatgacg
     1021 tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact gttctcttgt
     1081 gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa cacgattgaa
     1141 tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat ccataacaac
     1201 tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc cttcgaaaac
     1261 aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat caaatgctaa
     1321 ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa
     1381 tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct tacgcccgtt
     1441 ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca gttcttcttc
     1501 ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg aaaggacaaa
     1561 tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga cttgtcgaaa
     1621 taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca gctcatcaat
     1681 tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact caggccctct
     1741 catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta gtatagtgac
     1801 attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt aagtaaataa
     1861 atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac gtccagatcc
     1921 tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg tttcattcat
     1981 tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta ccaatagatg
     2041 gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct ctacgattgc
     2101 ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt cctcttcatc
     2161 aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga ttgcatttag
     2221 tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt ttacgccgct
     2281 ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat cgattcctaa
     2341 tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg ttcctatcgc
     2401 tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta ctttgaatgc
     2461 tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac gagggtgaac
     2521 aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa tgatcacgtc
     2581 tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt tatcaacagt
     2641 gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt ttggtccata
     2701 cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta attccagtgt
     2761 ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt aaacgaggat
     2821 tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca tatcgtgtat
     2881 cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga tatccttttc
     2941 ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt tcagttcacg
     3001 gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat caaaatttgc
     3061 ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag caaaaaaaag
     3121 cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca ggacctgatc
     3181 ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc tttgtttggg
     3241 cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg ttttcgcttg
     3301 taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact cgcgttgctg
     3361 ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg acatttcctg
     3421 agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag tcaaaaatac
     3481 aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg cagaaaagga
     3541 agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa gaacaataat
     3601 caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct tcacctactt
     3661 tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg tataaaattt
     3721 tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc tcatattgcg
     3781 gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt tttccaaata
     3841 gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg gattcactgt
     3901 cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga agccaaggac
     3961 tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc cagccataga
     4021 ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa atttttgtgt
     4081 caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc caaggtcgaa
     4141 tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat cgacgcgccc
     4201 gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc tgacgtctac
     4261 cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga caccttctac
     4321 aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg cttcataaca
     4381 gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat cttctaaatt
     4441 catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt cataatcgtc
     4501 gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt tcctaaatat
     4561 ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc aacttcagca
     4621 gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc tttattaaat
     4681 gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt atgtgttttt
     4741 ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac gaaatctttt
     4801 ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag tttgccgtac
     4861 agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc tcctttatga
     4921 tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt ttttgtataa
     4981 acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat gaatttcagc
     5041 ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc ctctatccct
     5101 tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct gctgatgagc
     5161 ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt accgattttt
     5221 ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca gtcgaacaac
     5281 caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt tctatgtttt
     5341 gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct gactttcttt
     5401 aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt cgagaatggg
     5461 ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta ctataaaaac
     5521 agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat ccagaacttt
     5581 cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca gcttctttcg
     5641 catgattttc agcttctttc gcatcatgca catctttttc gacatcttca taatccaagc
     5701 gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga ttgtcctctt
     5761 catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct acaaagtcga
     5821 acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc attcctccgg
     5881 caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac attccgatta
     5941 atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct attttgaatt
     6001 gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta atcgaacgaa
     6061 cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct acttgaaaat
     6121 ttaaaatgat tactcgtttg ctcattaaaa acgccccttt cataccttct tgttatttat
     6181 tttaccttta attgaaaaat aagaaaagaa aagcactctt tttttccaat aaaatagggc
     6241 tgtgacaaaa gtcgtatcac agctcctttt tccgaataaa cggcgttctt gaagctgatc
     6301 acttctgtca caacctccct taaatctgaa tctatatttt ttggtatttg tttttatttg
     6361 cttgcctatt cgacaaattc aaattcaaac tcaccgatac gtacaaggtc gccatctttc
     6421 gcaccgcgtg cccgaagagc ttcatccaca cccataccac gtaattgacg agcaaaacgc
     6481 atcactgttt catcatgatc gaaattcgtc atttggaata atttttcgat cttctcacca
     6541 gataagatcc acgttgcatc tgaatcacga tcgatttgga actcaggacc ttctggattg
     6601 aatccatagt gtacagtttc ttcttccagt tcttcttcgt acaatgggaa ttctggcgtt
     6661 acttctaaca aatctgctgt agcattcaat aacgcgtcca atccttgtcg agtcactcca
     6721 gaaattggaa atactgggat gtcatcagca aattcatctt ctttttcctt gttcagctgt
     6781 tctttgaatt tcactagatt ttccggtgca tccggcatat ccattttgtt tgccacgatg
     6841 atttgcggac gctctagcaa gcgtaagtta taagtagata attctttgtt gatggcaaga
     6901 tagtcttcat aaggatctcg tccttccatc ccactcatat cgatcacatg taaaattact
     6961 cgtgttcttt cgatatgacg aaggaattgt gtacccagcc caacaccttg cgaagctcct
     7021 tcaatcaagc ctggcaagtc tgctgcggca aaacttctgc catcacttgt agtaaccatt
     7081 cctagattag gtacgagcgt cgtgaaatga taagcgccga tttttggacg agcagatgaa
     7141 ataacggata gtaaagtcga tttcccgaca gatggaaaac ctacaagtcc aacgtctgcc
     7201 aatactttca attctagttc gatttttcgc tcttgtcctg gttcaccgtt ttcagcaatt
     7261 tccggtgcag gattccgagg agaggcaaaa cggatattcc ctcgtccacc gcgtccacct
     7321 tttgccacaa ctagtgtttg accattttca attaaatctc ctaaaagagc accagtctcc
     7381 gcatctcgga cagtggttcc ttgtggtact ttaacatagg tatcttctga accacgaccg
     7441 tgcatccctt tactcatccc attttcacct ggctgcgctt tgaaatggcg attgaaccga
     7501 aaatccatca atgtacgtaa cccttcatcg acgatcaaga tgacatcgcc gccacgacca
     7561 ccgtctccgc cagccggtcc gccgtcagga acgtattttt cgcgacgaaa tgcaaccatt
     7621 ccgtctccgc ccttgccagc tttcacatcg atcgttactt gatctaaaaa catggacata
     7681 attaagtcct cctattattc atttatttat ttgactgcct gaatttccac aataaacaaa
     7741 agtcttactt attttatcgg tgaaatctga ttttgtctat catttcagca aaaagattgt
     7801 ttaccttctt ctttttttat catttggtaa ggtttccaac cttttttctg ctaccaagta
     7861 taattttttg acaaaatatt ctttgcgtag cagaataaca gtgtattaat tgtattaatt
     7921 gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga cagtaaaagt
     7981 aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca aagaagtctc
     8041 agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt tagcacaatt
     8101 attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa cagaagactc
     8161 aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa gcaaaatccc
     8221 ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt atacatctga
     8281 agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt ctgccccagc
     8341 tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg aaaacgacaa
     8401 aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt atttcttaaa
     8461 taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca cttccacaca
     8521 aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt cagctgctga
     8581 caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta tccctgagct
     8641 acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt cattaactga
     8701 attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag caatcaaaaa
     8761 acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt ctagcgatgt
     8821 cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa caactgctgg
     8881 tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat ttacttgcca
     8941 aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa tcgataaaag
     9001 aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact tttaaactcg
     9061 ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt tcaggatctg
     9121 tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt ggtcccttcg
     9181 ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg tctccatgta
     9241 taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct tcaccgaaaa
     9301 agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga aatgctggaa
     9361 gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa taaccgcaat
     9421 cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc ataactgttg
     9481 ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg ttttctagca
     9541 ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca atgtaatccc
     9601 cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc atatcatgaa
     9661 acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga gccacaagag
     9721 ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc agaccgtctt
     9781 ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta tgtgcatccg
     9841 tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct ttctcagaag
     9901 gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca atcagagcta
     9961 aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct catttctctt
    10021 ttttcatata accttgaatt agtataatca aagaatctct actatgcaat gttcattttt
    10081 ataacccgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
    10141 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
    10201 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
    10261 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
    10321 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
    10381 agaaaagtca ttcaattacc tgctcatgaa gtagat
//
LOCUS       DACDCV010000093        10194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDCV010000093 DACDCV010000000
VERSION     DACDCV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.093"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..626
                     /locus_tag="IV041_002324"
     CDS             120..626
                     /locus_tag="IV041_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8383077.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="IV041_002325"
     CDS             643..1542
                     /locus_tag="IV041_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8383078.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="IV041_002326"
     CDS             1629..2513
                     /locus_tag="IV041_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8383079.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="IV041_002327"
     tRNA            complement(2705..2776)
                     /locus_tag="IV041_002327"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="IV041_002328"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="IV041_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8383080.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4373..5410
                     /locus_tag="IV041_002329"
     CDS             4373..5410
                     /locus_tag="IV041_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8383081.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="IV041_002330"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="IV041_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8383082.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="IV041_002331"
     CDS             6562..7752
                     /locus_tag="IV041_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8383083.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="IV041_002332"
     CDS             7856..8611
                     /locus_tag="IV041_002332"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8383084.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="IV041_002333"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="IV041_002333"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8383085.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3566 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct caga
//
LOCUS       DACDCV010000094         9902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDCV010000094 DACDCV010000000
VERSION     DACDCV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.094"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..592)
                     /locus_tag="IV041_002334"
     CDS             complement(368..592)
                     /locus_tag="IV041_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383086.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            792..3560
                     /locus_tag="IV041_002335"
     CDS             792..3560
                     /locus_tag="IV041_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8383087.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3641..4060
                     /locus_tag="IV041_002336"
     CDS             3641..4060
                     /locus_tag="IV041_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8383088.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4072..4542
                     /locus_tag="IV041_002337"
     CDS             4072..4542
                     /locus_tag="IV041_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383089.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4559..5338
                     /locus_tag="IV041_002338"
     CDS             4559..5338
                     /locus_tag="IV041_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8383090.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5325..6146
                     /locus_tag="IV041_002339"
     CDS             5325..6146
                     /locus_tag="IV041_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8383091.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6163..7212
                     /locus_tag="IV041_002340"
     CDS             6163..7212
                     /locus_tag="IV041_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383092.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7225..8229
                     /locus_tag="IV041_002341"
     CDS             7225..8229
                     /locus_tag="IV041_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383093.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8862..9881
                     /locus_tag="IV041_002342"
     CDS             8862..9881
                     /locus_tag="IV041_002342"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8383094.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3321 a         1643 c         2072 g         2866 t
ORIGIN      
        1 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
       61 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
      121 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
      181 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
      241 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
      301 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
      361 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
      421 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
      481 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
      541 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
      601 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
      661 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
      721 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
      781 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
      841 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
      901 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
      961 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
     1021 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
     1081 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
     1141 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
     1201 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
     1261 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
     1321 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
     1381 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
     1441 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
     1501 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
     1561 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
     1621 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
     1681 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
     1741 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
     1801 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
     1861 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
     1921 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
     1981 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
     2041 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
     2101 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
     2161 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
     2221 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
     2281 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
     2341 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
     2401 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
     2461 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
     2521 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
     2581 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
     2641 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
     2701 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
     2761 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
     2821 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
     2881 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
     2941 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
     3001 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
     3061 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
     3121 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
     3181 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
     3241 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
     3301 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
     3361 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
     3421 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
     3481 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
     3541 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
     3601 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
     3661 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
     3721 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
     3781 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
     3841 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
     3901 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
     3961 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
     4021 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
     4081 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
     4141 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
     4201 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
     4261 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
     4321 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
     4381 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
     4441 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
     4501 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
     4561 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
     4621 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
     4681 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
     4741 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
     4801 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
     4861 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
     4921 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
     4981 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
     5041 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
     5101 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
     5161 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
     5221 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
     5281 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
     5341 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
     5401 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
     5461 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
     5521 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
     5581 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
     5641 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
     5701 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
     5761 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
     5821 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
     5881 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
     5941 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
     6001 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
     6061 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
     6121 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
     6181 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
     6241 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
     6301 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
     6361 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
     6421 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
     6481 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
     6541 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
     6601 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
     6661 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
     6721 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
     6781 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
     6841 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
     6901 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
     6961 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
     7021 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
     7081 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
     7141 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
     7201 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
     7261 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
     7321 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
     7381 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
     7441 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
     7501 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
     7561 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
     7621 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
     7681 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
     7741 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
     7801 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
     7861 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
     7921 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
     7981 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
     8041 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
     8101 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
     8161 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
     8221 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
     8281 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
     8341 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
     8401 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
     8461 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
     8521 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
     8581 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
     8641 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
     8701 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata
     8761 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
     8821 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
     8881 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
     8941 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
     9001 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
     9061 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
     9121 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
     9181 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
     9241 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
     9301 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
     9361 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
     9421 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
     9481 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
     9541 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
     9601 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
     9661 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
     9721 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
     9781 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
     9841 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
     9901 tc
//
LOCUS       DACDCV010000095         9846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDCV010000095 DACDCV010000000
VERSION     DACDCV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.095"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..3256
                     /gene="fss3"
                     /locus_tag="IV041_002343"
     CDS             29..3256
                     /gene="fss3"
                     /locus_tag="IV041_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8383095.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3369..3683
                     /locus_tag="IV041_002344"
     CDS             3369..3683
                     /locus_tag="IV041_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8383096.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3696..4070
                     /locus_tag="IV041_002345"
     CDS             3696..4070
                     /locus_tag="IV041_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8383097.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4080..4415
                     /locus_tag="IV041_002346"
     CDS             4080..4415
                     /locus_tag="IV041_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8383098.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4498..5838
                     /locus_tag="IV041_002347"
     CDS             4498..5838
                     /locus_tag="IV041_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8383099.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5916..6590
                     /locus_tag="IV041_002348"
     CDS             5916..6590
                     /locus_tag="IV041_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8383100.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6823..7257
                     /gene="dcm"
                     /locus_tag="IV041_002349"
     CDS             6823..7257
                     /gene="dcm"
                     /locus_tag="IV041_002349"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8383101.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7258..7965
                     /locus_tag="IV041_002350"
     CDS             7258..7965
                     /locus_tag="IV041_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8383102.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            7955..8245
                     /locus_tag="IV041_002351"
     CDS             7955..8245
                     /locus_tag="IV041_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383103.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8504..9688
                     /locus_tag="IV041_002352"
     CDS             8504..9688
                     /locus_tag="IV041_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8383104.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9703..9822
                     /locus_tag="IV041_002353"
     CDS             9703..9822
                     /locus_tag="IV041_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8383105.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3256 a         1663 c         2238 g         2689 t
ORIGIN      
        1 tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt
       61 attaactatt ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac
      121 ttttgctgat gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca
      181 atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca
      241 ttatgcagtg tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat
      301 ttacaatgag ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc
      361 caaattagtt tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc
      421 acagaagatg atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga
      481 tggtagtgcc gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta
      541 tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac
      601 tgtgacggat acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc
      661 aaacattgat tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa
      721 agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa
      781 aaaggctggc caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat
      841 caaaattgat gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg
      901 tgatgttgta ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga
      961 cgtgactact gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt
     1021 aaccattacc gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac
     1081 caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca
     1141 aatcattctg gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc
     1201 cttagctggt aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga
     1261 aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga
     1321 attgggaacc tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa
     1381 acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt
     1441 aaaagggcaa aatcaagaag tcactggcga aaccactttg acgaaagaag acaaagatac
     1501 tggggataaa gcacaaggta aggcagtctt tgagggtgct gaatacacct tgtttactgc
     1561 taaggatgga aaagctgtta aatggagtga ggcttttaaa ccagagttag cgaagggaac
     1621 gaaaggttct gatgaaacgg tcactttgac tttagacgaa aagaaccaag ttgccgttaa
     1681 acatttagct atcaacgagt attattggca agaaacaaaa gcgcccgaag gatatagcct
     1741 tgatgaaaca aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt
     1801 gattacccga aatgttacgg caaaagaaca agtgattcgc tttggctttg atttctttaa
     1861 atttgctgga tcagctgcgg gcactaccga aactggcttc aatgatctga cctttaaagt
     1921 gtcgccattg gaagggacca atgaaatcac aggtgccgaa gatgaagcga ccacagctta
     1981 taacgagcaa ttagggtttg atggttatgg caagtttgaa aatcttcctt atggggatta
     2041 tttacttgaa gaagtagaag ccccagaagg ttttcaaaag attacgccat tagaaatccg
     2101 ttctactttt aaggaaaaca aagaagactt tgtgaagagt gagtatgtct ttaccattac
     2161 cgaacaagac caaaaacaac caattaaaac ggtgaccgtt ccttacgaga agctgacgaa
     2221 caaagcattt tctgttagtt tgaaccgttt aatgctctat gatttgcctg aggaagaaga
     2281 tagtttaact tctcttgcga cttggaaaga cggaaacaaa gaattaactt ctctggattc
     2341 taccgagcta gttgataaat tgagttacaa tctacatgaa atcaaagaag actggtatgt
     2401 ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc
     2461 caaaccagtg gtgattgcag aaacaagcgc aacgttggcg aacaaagaga aaacaggaac
     2521 ttggaaaatt cagcataaat taaccgccga acaagtttta aataaaacta ttgtcttatt
     2581 caactatgtg tatgaaaata aggaagcttt tgaagcaggc gataagccag tagcgaagga
     2641 tgttagcttg aataaccaag cccaaactgt cagttgtaca gtggaacacc atgtttctat
     2701 ccaaacaaag gcccacctag aaaacggttc tcagaccttt actcatggtg atgtggtgga
     2761 tatgtttgat gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagctttcga
     2821 aacaattctg tatgctttat taccggatgg tacaaacaaa gaaatttgga aatctggcaa
     2881 gattgattat gaagtaaatg ataaagaatt caccaaaact gtgcttgcga aaaaagtaga
     2941 taccggaaaa tatccagaag gaaccaagtt tacttttgcg gaaatcaatt acgataaaga
     3001 tgggaatatc aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc
     3061 aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag
     3121 tggctctcaa gaatctagtc ccacagtgaa gaccttccct caaaccgggg agaaaaattc
     3181 caatgtttta ctgttcattg gttttacttt gatttttgcg acggcgggct attatttctg
     3241 gaatcgccgg aactaaggtg atgagatgat acaagtaaat cctaagaacc aacgtgcgcg
     3301 accgcctcct aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg
     3361 aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa
     3421 gttatgccgg tgaaggagaa attataacag aaggatatgg acggaatact acggtgattg
     3481 gtcgcagtta tcatttgtat tccagtaagc aacgggccga tgatattgag gtggtagtag
     3541 ctgcggaagc cggtgaaaag gattttgatc aagaccaacc gctaaaagcc gtgaatcccc
     3601 atttggttgc caaaggctat gagattgaga atcgtgggtt tactgattac gtgttgtatg
     3661 ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg
     3721 tagattcagg tttaacattt gggaagttgc gtttttccgc attacgccga gaagtacgga
     3781 agcaaaatga ggatggaaca attggtaatg aggtaaaaga acgaacctat aatttaaaat
     3841 cctctgcaca agggcgaatg attcaagtta gtatcccagc taatgtaccc ttacgcgagt
     3901 ttgcttatga tgcagaggta gagttggtca atccaatcgt ggataccgtt gccaattatg
     3961 tttttcgaga aggaactacc gttaattggt tcattaaggc ggatgattta gtcttgaagc
     4021 ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag gtgaagatta
     4081 tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa ggcgagctga
     4141 ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa aatgtttctg attatacatc
     4201 aaggtttact tggagaaaat agcgacagtg aagaagactc tgatgtacta gactggttag
     4261 gagaagaaga gtctttcttt atagttgtcg aagtaaatga aaacttatgt cggcaggcag
     4321 aatccatttt ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc tttttaaatc
     4381 aattggttga aacgaaacaa ctcccctcct cgtagcagta aatgactagc ataacttttt
     4441 gaaattgcct aatgaaatcc tattgggctt tttcgattgg aggtggaaac catcaaaatg
     4501 tacaaaggac atcgcatacg cgcaggagat cagcacttgg tttatcactt tgttcttggc
     4561 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gtttagacaa
     4621 tttgatattt ctaagctatc actttctact attgaaatcg tttggtctat aaaagattta
     4681 gtatgtctgc ttggaagtct cgctttttca ggagctatga tattgcttta tattcacttt
     4741 tttcttgatc attggcgaag cttgtggcat cgacaaaagc tagctcggat gattctagag
     4801 aaccattggt atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga tttgaatagt
     4861 agccgaacca gagagactat cagttacttc cccaaaatct gttatcgaat gaaagatgga
     4921 ttactgtcta ttcgcgttca aatttcactt gggaaatacc aagatcagct tttaaaattg
     4981 gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact caaagactcc
     5041 tatgtggagt atactttact gtatgatatg atcgccaatc gaattgggat agacgaagta
     5101 gtggcagaaa atgggactct gcgattgatg aaaaatcaag tatgggctta tgattcttta
     5161 ccccatatgt taatcgctgg tggcacgggt ggagggaaga cctatttcct cctcaccatt
     5221 atcgaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat
     5281 ttagctgatt taggtacggt gatgcctcat gtttattcac aaaaggaaga aatttctgct
     5341 tgtgtggaag atttctatga acgtatgatg gctcgtagca aggcaatgaa agaaatgtct
     5401 aactacaaaa caggagagaa ttatgcctat cttggacttc cacccaactt tttaatcttt
     5461 gatgaatacg tggcttatat ggagatgtta acgacgaaag aaagtgcggt gattttgaat
     5521 aagctcaaac aaatcgtgat gttgggtcgt caatctggtt tctttctgat tttggcttgc
     5581 caaaggccgg atgcgaaata tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg
     5641 gctttgggtc gtatgagtga actcggatat tccatgatgt ttggggaggt cgaaaaaaac
     5701 ttctttatga agcacatcaa aggtcgaggt tatgtggata caggtggcag tgtgattagt
     5761 gagttttata cgccacttgt accaaaaggg tatgatttct tagattcaat tagtaaatta
     5821 gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt aacgtataat
     5881 acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat tcttatttag
     5941 agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg aaatcccccg
     6001 aagttggacg agacgcagtt tttatggcag ggagtcctgg ggctggaaaa tcagaggttg
     6061 ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat tactttcgca
     6121 cccagtttcc tgattataat ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt
     6181 tggtagagca ggcatttaga tatgtcttag atcatggcta ttcttttatt ctggatgcaa
     6241 cttttgcaat attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag
     6301 tgacgatttt ctatgtttat caagacccta aaatcgcttg gaaatttaca caagaacgtg
     6361 aaattgcaga gggacgtcat gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa
     6421 gagagaacat tgcaaaagta aagagtaaac atccagatgt attgcttcat atcataataa
     6481 aagattatac gaataatatt tccgaagttc attatgctac ggataatcta gaattatttt
     6541 taccactagg gtatagtagg aaagatttgg aggttcaatt acatgactga gaaagaacaa
     6601 gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg atttatcgga aaatgctact
     6661 gcaaaagagt tcaaaacggt gattaaatat gtagccgatc aggccaatga aaaacaacgg
     6721 aaattagttg gattaaataa aaaatagtag gacaaattaa taatgtatga gccaagagca
     6781 aaatgttctt ggcttttttc tttgcaatga atgaggtaat gtatgagatt tttagattta
     6841 ttcgccggta ttggtggctt tcgtttaggt atggaacagg cgagccatca ttgtatcggt
     6901 ttttgtgaaa ttgataaatt tgctagacgt agttataaag caattcatga tacaagtaag
     6961 gaggtggaaa tgcatgacat cacaagtgta tcagatgagt ttattcaatc cctcggacca
     7021 gtggacatcc tttgcggcgg atttccgtgc caagcttttt caattgcggg aaagcggcaa
     7081 agattttccg atactcgagg tactttattc tttgaaatcg ctcggttcac cactcttctc
     7141 cgacctaagc ttttattcct tgagaacgtc cgaggattgc tcaatcacga aggaggggct
     7201 acgttcgaga cgatcctccg aacgctggat ggattggggt atgacgtgga gtggtaggtg
     7261 cttaactcaa aggcctacat tccccaaagc cgggagcgga tattccttat cggacattct
     7321 cgagacgcat gtaccgaaca agtatttcct atcattggac cgtcttcaac atctgatcaa
     7381 aacatcagaa atttggtaaa tatcaatcct tccaatcgag gaatgggtgg acaagtctat
     7441 ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca
     7501 gcaagggatg gaatctctgt agcaggacta ttaccgggaa agtttgaaca aggtaatcga
     7561 gtttatgaag ttactggaac cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc
     7621 atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc cagaagacag tgtgaatctg
     7681 gcttttccta attccacaag tcgccgaggt cgtctgggaa aacagagtgt tcatacctta
     7741 ctaacaggag accaacaagc gatcgtcacg gatcagtatc aaattcggaa gctaacacct
     7801 agagagtgtt ggcgacttca gggctttccc gattgggctt ttgatcgaga ttctcaagtc
     7861 aattcagata gtcagttgta taagcaagcc ggaaactctg taacagttcc ggtcattttt
     7921 gatattgcca gaagattaaa agagttggat gtacatggtt tatgatgagt tgattggaga
     7981 aatttattgg gtgattggaa agattcagtc agacccagaa ttagaagaac aattgcgacg
     8041 gttaaaattt gatattcgca agaatggtgt caaagtcgct ggcgatccat atttaatgaa
     8101 tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg agcggattgc
     8161 ggaccaagca aaagaaccag atattcgtca atacttgtta gaaatgaaag cagagttgga
     8221 agtaaatggt attacagatg agtaacctaa accaacacaa ctgactgtta ggcgctgacc
     8281 gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcgtta
     8341 gccgaccatg atgtctcacc cccgtatcta acaggggggt acaaaaacac aaaaaataag
     8401 taagcaacca ttaagaatgc ttgttgtatc aaagatagta agtaatttgt ataggtgtta
     8461 actcatttga aaaagttaac accttgaatt tggaggtgag aacttggcac aacggaattt
     8521 agattatcgg ctattaaaag accgacgcaa cgaatacggc gtttcacaaa ataaattggc
     8581 tacggcttgt ggacttagtc ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc
     8641 atctataaaa accatgagga aaatttttga ccaactggaa agctttaatc ccgatttgcc
     8701 tttgacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat
     8761 tattcaagaa attctccatt tgaaatttga gtatatgctc catgaagatt atgcctttta
     8821 ctcctatcaa gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga
     8881 taaaggtgtg cttttagaat taaaggggcg aggttgccga caatttgaaa cttttttact
     8941 cgctcaaaag cgtagctggt acgacttttt cgaggattgt ctaaaggccg gtggcgtgat
     9001 gaaacgcttg gacttggcaa tcaatgatcg agtggggcta ttggatatac ctgagctaac
     9061 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt acctttaaaa gttatcgttc
     9121 cggggaactt ttaaaggctg atgaaaagga cggcatgggc aataccttat atatcggcag
     9181 tctcaagagt gaggtttatt tctgtttgta tgaaaaagac tatgaacaat atatcaagtt
     9241 agggattcca ttagaccaaa ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga
     9301 ccgtgctttt catgcgattc aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt
     9361 ttccattatc aatcgttact tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa
     9421 ctggcccttg aatgaacaat gggggcgttt tattggacgg aatcggaaag agattcaatt
     9481 aacttccgag ccaaaaccgt ataccattga acgaaccttg aattggctag gccgacaagt
     9541 cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcggacaa cctatatcca
     9601 ggatatggta cagaatgcac gcttatcaga ccgacataaa aagattttgg agcaacaaag
     9661 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat
     9721 atttttttgg tatttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgaatgca
     9781 agtgcagcag cggatcgagc aatggaaaag aaaggaaagt gacaagatga atttcggaca
     9841 aaattt
//
LOCUS       DACDCV010000096         9845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDCV010000096 DACDCV010000000
VERSION     DACDCV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.096"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IV041_002354"
     CDS             185..1183
                     /locus_tag="IV041_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8383106.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IV041_002355"
     CDS             1295..1804
                     /locus_tag="IV041_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8383107.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IV041_002356"
     CDS             complement(1834..2493)
                     /locus_tag="IV041_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8383108.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IV041_002357"
     CDS             complement(2495..3259)
                     /locus_tag="IV041_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8383109.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IV041_002358"
     CDS             complement(3267..3944)
                     /locus_tag="IV041_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8383110.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IV041_002359"
     CDS             complement(3981..5366)
                     /locus_tag="IV041_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8383111.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IV041_002360"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV041_002360"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IV041_002361"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IV041_002361"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8383112.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IV041_002362"
     CDS             6305..7162
                     /locus_tag="IV041_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8383113.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IV041_002363"
     CDS             complement(7243..7578)
                     /locus_tag="IV041_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383114.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IV041_002364"
     CDS             complement(7645..9015)
                     /locus_tag="IV041_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8383115.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IV041_002365"
     CDS             complement(9093..9629)
                     /locus_tag="IV041_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8383116.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2924 a         2019 c         1634 g         3268 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggc
//
LOCUS       DACDCV010000097         9703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDCV010000097 DACDCV010000000
VERSION     DACDCV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.097"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(113..643)
                     /locus_tag="IV041_002366"
     CDS             complement(113..643)
                     /locus_tag="IV041_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383117.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(742..1092)
                     /locus_tag="IV041_002367"
     CDS             complement(742..1092)
                     /locus_tag="IV041_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8383118.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1249..2778)
                     /gene="ahpF"
                     /locus_tag="IV041_002368"
     CDS             complement(1249..2778)
                     /gene="ahpF"
                     /locus_tag="IV041_002368"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8383119.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2788..3351)
                     /gene="ahpC"
                     /locus_tag="IV041_002369"
     CDS             complement(2788..3351)
                     /gene="ahpC"
                     /locus_tag="IV041_002369"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8383120.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3495..4157)
                     /locus_tag="IV041_002370"
     CDS             complement(3495..4157)
                     /locus_tag="IV041_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8383121.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4341..5987
                     /locus_tag="IV041_002371"
     CDS             4341..5987
                     /locus_tag="IV041_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8383122.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5978..6106
                     /locus_tag="IV041_002372"
     CDS             5978..6106
                     /locus_tag="IV041_002372"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383123.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            6073..7137
                     /gene="uxuA"
                     /locus_tag="IV041_002373"
     CDS             6073..7137
                     /gene="uxuA"
                     /locus_tag="IV041_002373"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8383124.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(7245..8798)
                     /locus_tag="IV041_002374"
     CDS             complement(7245..8798)
                     /locus_tag="IV041_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8383125.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            8946..>9703
                     /locus_tag="IV041_002375"
     CDS             8946..>9703
                     /locus_tag="IV041_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8383126.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNP"
BASE COUNT         3185 a         1789 c         1755 g         2974 t
ORIGIN      
        1 taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag
       61 atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta
      121 tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa
      181 taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga
      241 atccgccaat caacaacaga atactactat aaacactcca acttttagct tttgtattca
      301 tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga agaatcggca
      361 ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa
      421 gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa
      481 aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa
      541 aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta
      601 ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt
      661 gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg
      721 agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc
      781 tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc
      841 ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg
      901 ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt
      961 ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt
     1021 tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg
     1081 attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat
     1141 aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct
     1201 tgtcaccatc cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat
     1261 cagataatcg aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg
     1321 tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc
     1381 atttttctcg gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc
     1441 cgtattaggg agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc
     1501 catcgtacgg cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt
     1561 cgtcgctgca tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat
     1621 ttgatctgct tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc
     1681 caaatcgatt gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc
     1741 tttaaataaa gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt
     1801 ttcgcctggt acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc
     1861 tttaagtgat ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga
     1921 aacggcctgt tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg
     1981 ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt
     2041 ttcaacgact tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc
     2101 tgcatagata gctgcactac tagctgctgg tccaccgcca attactaata catcataagg
     2161 ttctttttca aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc
     2221 gatcgtcatg cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat
     2281 gattttttta tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat
     2341 atttggattt aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca
     2401 agtcaagcta acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact
     2461 caaagattca ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga
     2521 ggaaaattca tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg
     2581 attgatagaa aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga
     2641 catttctgct atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa
     2701 actaacagaa aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt
     2761 agtttctttg tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt
     2821 caatgtttcg ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac
     2881 atattgagca gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc
     2941 attgatttcc atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc
     3001 taatccttgc tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc
     3061 gatcatcacg tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt
     3121 aaagtgggta tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta
     3181 ttgttcttgt aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa
     3241 gcaaagaata ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg
     3301 atgataagca tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc
     3361 tccaataata attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg
     3421 atattttgtt atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag
     3481 gtcgcaagaa aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt
     3541 tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta
     3601 tcaactaaag ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc
     3661 tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag
     3721 ttccaaactt cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca
     3781 cggaaaaaag cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt
     3841 tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct
     3901 tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca
     3961 tacgtaccac tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct
     4021 tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc
     4081 cttccaggtt ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat
     4141 tgcaagctgc ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga
     4201 agaggtctga aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa
     4261 attttggttg acatagcgat ttttattgta taaaattcac ttgaatacaa aaactaatat
     4321 gttagtttag gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat
     4381 tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac
     4441 aaaaaaatcc tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag
     4501 aaatcgctca acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa
     4561 cattcgatga agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag
     4621 tggtcatgcc aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc
     4681 tgtattgtca tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc
     4741 ctagtttaca attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa
     4801 ataataaata tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac
     4861 atacaatgag tattcttgct gcgctacttt ataaacgttt tttatctgga gcttggccga
     4921 ttgcacttgt ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc
     4981 taacgatcgc aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact
     5041 tatcgaatga agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc
     5101 cttccttgtc agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc
     5161 aaaccgcaaa aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa
     5221 tcatggaaga tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga
     5281 ctgatcgtgc aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt
     5341 tgcatacgtc tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga
     5401 tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag
     5461 ttgtggagtc tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc
     5521 gcttttctaa tccaaatatt cctgatatgc ctcagcgaat cgccacggat acctctcaaa
     5581 aggtggcgat tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc
     5641 cgcagacact tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca
     5701 ttgatgatca aggaaaacca ttcatcccga gtccagaccc actattgaca gactttcagc
     5761 ataagctttc cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg
     5821 ctcctatttt gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca
     5881 gaaaaataga agaaatgttc atcaagatgg tagcaggacc gaatgccgta cgaaaaacat
     5941 tacaggatta cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta
     6001 cggatcacag gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg
     6061 gattgtaggc acattgatgg atcacaggaa gaaaagatta ccttagatga aatcagacag
     6121 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
     6181 cccagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
     6241 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
     6301 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
     6361 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
     6421 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
     6481 gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga acctgagcgc
     6541 ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
     6601 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
     6661 atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg aatcgtaaag
     6721 aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa cggaatcact
     6781 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
     6841 ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
     6901 cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat gtaccgcata
     6961 ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc gtcctgatca tggaagaatg
     7021 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
     7081 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
     7141 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
     7201 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
     7261 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
     7321 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
     7381 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
     7441 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
     7501 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
     7561 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
     7621 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
     7681 tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat gaaagtagga
     7741 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
     7801 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaaag
     7861 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
     7921 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
     7981 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
     8041 ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
     8101 gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt atctatcata
     8161 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
     8221 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
     8281 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
     8341 gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt cattttcgta
     8401 tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga atttgtctga
     8461 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
     8521 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
     8581 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
     8641 atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata ttgtgtagta
     8701 ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt gctttttttc
     8761 ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
     8821 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
     8881 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
     8941 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
     9001 ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca gaaaccaagt
     9061 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
     9121 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcaggagact
     9181 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct ttgtcgattg
     9241 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
     9301 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
     9361 cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
     9421 ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
     9481 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
     9541 gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa cctgaaataa
     9601 cccctggaga aaaaccagat gtagacccag aagaaaaacc agatgtgact ccagaaccag
     9661 atacggattc tggaaaccaa acagtaccgg aaacaaatcc aga
//
LOCUS       DACDCV010000098         9547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDCV010000098 DACDCV010000000
VERSION     DACDCV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.098"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(157..939)
                     /locus_tag="IV041_002376"
     CDS             complement(157..939)
                     /locus_tag="IV041_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383127.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1004..1840)
                     /locus_tag="IV041_002377"
     CDS             complement(1004..1840)
                     /locus_tag="IV041_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8383128.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1858..3267)
                     /locus_tag="IV041_002378"
     CDS             complement(1858..3267)
                     /locus_tag="IV041_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8383129.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3281..5104)
                     /locus_tag="IV041_002379"
     CDS             complement(3281..5104)
                     /locus_tag="IV041_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8383130.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5947..6273)
                     /locus_tag="IV041_002380"
     CDS             complement(5947..6273)
                     /locus_tag="IV041_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383131.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6319..6681)
                     /locus_tag="IV041_002381"
     CDS             complement(6319..6681)
                     /locus_tag="IV041_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8383132.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6718..6933)
                     /locus_tag="IV041_002382"
     CDS             complement(6718..6933)
                     /locus_tag="IV041_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383133.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7060..7395
                     /locus_tag="IV041_002383"
     CDS             7060..7395
                     /locus_tag="IV041_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383134.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7471..9468)
                     /locus_tag="IV041_002384"
     CDS             complement(7471..9468)
                     /locus_tag="IV041_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383135.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2937 a         2002 c         1430 g         3178 t
ORIGIN      
        1 actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat ccgaaaaaaa
       61 atacccccag tagcttatcc taagctactg gagatgtata agtacggtca tataacaacg
      121 gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt ttttaaatac
      181 ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt ttttcccatt
      241 ttcaagtaac cgaagtggaa tgtctttacc aacttgctct gtcactgcat tactgatacc
      301 tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct ctgtatcagt
      361 atttatcgtg attcctttat cttcatttac aagcttccaa gtaggtggta tagctgtgta
      421 tgtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta cttctgtctc
      481 attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg cttctgtctc
      541 attattctca ccggaaactc tcttcacacc caaacttata tattgtccat ctttagtgtg
      601 catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc tttcaataga
      661 gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta aaagcatttt
      721 gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta tacggaatac
      781 agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc aatctccaag
      841 tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac tggtagaggt
      901 cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag ccttagcaca
      961 accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac acccttgaaa
     1021 tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc tcttcttgaa
     1081 cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc ttcactgaag
     1141 ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg gcaataaaaa
     1201 actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc tttttttcgg
     1261 gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag agcatggttt
     1321 cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg tttccagcct
     1381 cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca tagcagccga
     1441 tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt tgaaaaacaa
     1501 tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta aaaaatagac
     1561 ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc ttgacgcaaa
     1621 taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt agagcatcag
     1681 aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa tcgcctttct
     1741 ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc ttatctaagc
     1801 aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc gttctctcta
     1861 agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt tttttattcg
     1921 ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat aacgcttatt
     1981 gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac caaaaacatc
     2041 tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat aatcaattcg
     2101 atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat agccattttc
     2161 agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta atcgtagtcc
     2221 agtagggtca actacccagc catatggcgt tttatctaca taaggatttt cagctgcccg
     2281 gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg aaacatctga
     2341 tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg caataaaatc
     2401 gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat ataatccacg
     2461 atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta catctaagaa
     2521 agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg gactggaggt
     2581 agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa gattcaactg
     2641 atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa tttgaaagag
     2701 ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag gatgaaggat
     2761 catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat actcagtaag
     2821 caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc aaccgccctg
     2881 ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt cgattccctg
     2941 attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat tggttttatt
     3001 attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt ttaagcctaa
     3061 ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg caacttcaaa
     3121 attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt tcccatcctc
     3181 ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa acttctctgg
     3241 aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt tgcatcaatg
     3301 gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt tcactgttgg
     3361 tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg aaatcggcgt
     3421 tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact tcaaagggtt
     3481 ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata ccttctttag
     3541 aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc tcaacaggtg
     3601 aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt cccttagaaa
     3661 agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga ttttcaacaa
     3721 tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca aatccaatta
     3781 gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt gcgtgaacaa
     3841 tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg gaaacaaagg
     3901 catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca ccaatcatgg
     3961 aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc tcagtaattc
     4021 ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg tttttagttt
     4081 tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc attcctggcc
     4141 cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc gtcattgccc
     4201 aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat ggaattaacc
     4261 aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc agcaacgttc
     4321 caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc agcatcggct
     4381 tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct acataggaca
     4441 tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca gctaataccg
     4501 gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata tttggatgaa
     4561 gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt atggctgccc
     4621 cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta ttcaaaatga
     4681 tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc aaaatggctt
     4741 ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta gaaatgacac
     4801 taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt ttaattgaag
     4861 ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca atcaccactt
     4921 ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct aaggctttcg
     4981 tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa tgggttaagc
     5041 tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact tctctgtaat
     5101 ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc cccaatcaac
     5161 attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc aataacgtta
     5221 ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga atttaatttt
     5281 ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca actagggtat
     5341 atctgcgtat acttactgct aaaataagta tatctaatat taactatacc agtaaaacca
     5401 ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat gcatataacg
     5461 gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg tgagtaaagg
     5521 atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt tttttggtaa
     5581 aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc gatagaatga
     5641 tttgggctag tattcttaaa aattaactag caaatgttta aactgattta tttctaatct
     5701 acatgtagta agtatttcaa ttccaccaat tatcaataaa aataccaaac atatccataa
     5761 taattttttc tttttcattt cttttatatt gttataacag tgaataacat aaaaattccc
     5821 gacaatcatt gtaatatttt tttgtcacct gtattagtat ttaatttata ggaaactata
     5881 aaaactccct agtgaattta cacctttggg agttcctgtt tggacggtat gcataataca
     5941 tttaaatcaa tttaacatta acacctcagc atatggtatc tccacatata tacggtcttt
     6001 ctttggatag tactcaatat ttatgttgat agcacttcca ttgtctaata tagctttagc
     6061 ccggtatgtg ttatctccat tttcatgagt aatcgtcaca tcatcacatg atcgatttaa
     6121 gttgtactgt tcttgtaaaa tctgtgttac caatggtttt gatgacttct ctaatccaga
     6181 tgtacctgtc atttttgaaa aaataatacc tataatagcc aagacaacaa tacctataat
     6241 gacaaacttc ttgttcatat ttactttatt caacttttta tctcctatct ccaaaataat
     6301 ttgctcttat ccctcttatc agtacattga catccctaaa agtacaccaa agattgctcc
     6361 agctacccct gctatcatca aaatcgtacc cattttctca tctttttctc gatataagta
     6421 gcccataata acagcaacag caccaaatac aacaggaata aatgctaacg atactaccat
     6481 agaaatccaa ccaataatca aatacagctt ccctgtctct tgtccatgta cgatttgttc
     6541 cttcatttca ttaattgtct gctcagtact tttctttact gtgtctgttc cggttgtctc
     6601 atctgtttct tgtagtgctc cacaaaactg gcaatactta ctttcatctg ttatttcttt
     6661 gccacatttt ctacaataca tgtcttctcc tcctaaaatc attcaattgc ttattagtta
     6721 actgtttact ttattaaata catcagaaaa cagctcattc attggtacac ttaatatctc
     6781 agaaagcatc ttcatttgat agggtttagg aatcgttctc cctgtttgcc acgaacttac
     6841 acattgctgt gtaacattca ttttttttgc taactccgtt tgagaaagtc ctttttcttt
     6901 tctgtatcgg ctgagattac ttactttttt catgattctc ccctcctgtt ttattatata
     6961 caacaatatg atgtatttca actcataaaa tataaaaaac ttcaaatacc attacaaata
     7021 caacaacttg ttgtactata ctgagatggg ggtgtattaa tgaaacagaa acaatatgca
     7081 aaggtcatta gagaaaagcg aaaagctctc gggtggacac aaaaagaatt agctgaaaaa
     7141 atcttctcta cacaacaggc agtagcaaga tgggaaaatt ctgtaacaga gcctaacctc
     7201 gatagcttaa cagctctttc tagagcccta ggaacccctg taagccattt tctagacaat
     7261 gttgtcgtag attatgagga agagtttttg gctctctatc gttctcttag cactgaagat
     7321 gcagttcgaa caattgacta tatgaagcta ttaaaaaggc aagaaaatga acgcaatcag
     7381 ctattgaaag attgaaaaat aaataaccca ccgaacttta acggtgggtg tattgagatg
     7441 aaataagatt aagagctatg gataacctgt ctagtttaat acaattgggt cttttctctt
     7501 ccttactctc cgtccatcat ctcttgttac acttgtaaat tgatacagag gaactatccc
     7561 agctcctgat aatatcaatt cttttattgt ttcatctatc cctgcccctt tcgctaaata
     7621 tattaaaaaa ctactacttg ctttcacatt gtatagtttc ttaatatcct tagaagctcg
     7681 ttcaagagtg aatccttgtt taacaaagta gcctctgttt tttattaaag catttttcac
     7741 gctgttatcc ccaacattac gtaggcgttc acaaacagtg ttaataagtt ctttctctgt
     7801 cattagatat gtcccttgcc acgataagcc ctgtaaaact tcatgtgctt catctaaaat
     7861 caattgtttt tccttgctga gttctgatcg ggaattttga ggaaatactc ttttagcctc
     7921 tttttcgtta aaacatatct taacccattc ataagaaaaa tattgatatg taaaatgaat
     7981 tttttccaga agtggaagga tatcttcatt caatttttga atcacagaag ttgcattaaa
     8041 aacgtctaat cgatagacat tgcttctctt atgattaaag tttcctaacg ctttctcttc
     8101 ctttaaccta tttaggtatt cgagcatctt caaaggaaca tcttcatcat ctaatttttt
     8161 tatcaatcct ataaaagtca taagtgatag taacttatta catttatcta tgtcataagt
     8221 tttaccatag taagaactca tttttttagc taattgttcc cccgacaaaa tacaagtcac
     8281 actttcctta ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg
     8341 catttcctct agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt
     8401 gtcaaaaagt acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag
     8461 gttctctaat agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac
     8521 aactccaata aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg
     8581 cccagtttta ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct
     8641 taagttttta cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat
     8701 ctcattaaaa gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc
     8761 ttctttgtcg tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc
     8821 tctccctgaa tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc
     8881 agtaatatct aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc
     8941 aaaaaacatt ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa
     9001 gtattcgtaa acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac
     9061 aatcttgtat ttttctagat tttcagagta ccgaatactt ttttgtatcg caaacgcaga
     9121 attatgaact aaattgatag ataaaacctt ttctacgctt aagtatctac tatcatcttt
     9181 actcaaatct actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat
     9241 tactctggtt tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat
     9301 aaatgattgt gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt
     9361 atctaaacac ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa
     9421 tttttcttga tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc
     9481 cttataaaat tctgttaaag actaaaacat acagataata aaaacaaact ctttattact
     9541 atatgtt
//
LOCUS       DACDCV010000099         9418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDCV010000099 DACDCV010000000
VERSION     DACDCV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.099"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(32..454)
                     /locus_tag="IV041_002385"
     CDS             complement(32..454)
                     /locus_tag="IV041_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8383136.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(444..2618)
                     /locus_tag="IV041_002386"
     CDS             complement(444..2618)
                     /locus_tag="IV041_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8383137.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2634..4976)
                     /locus_tag="IV041_002387"
     CDS             complement(2634..4976)
                     /locus_tag="IV041_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383138.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4998..7541)
                     /locus_tag="IV041_002388"
     CDS             complement(4998..7541)
                     /locus_tag="IV041_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8383139.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7551..7949)
                     /locus_tag="IV041_002389"
     CDS             complement(7551..7949)
                     /locus_tag="IV041_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383140.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8030..8314)
                     /locus_tag="IV041_002390"
     CDS             complement(8030..8314)
                     /locus_tag="IV041_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383141.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8338..9336)
                     /locus_tag="IV041_002391"
     CDS             complement(8338..9336)
                     /locus_tag="IV041_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383142.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2681 a         2012 c         1469 g         3256 t
ORIGIN      
        1 tttttttcaa cgtattctct ttcaattttc actaaaaatt aggaagaaat tgattcaatg
       61 tcttttcaaa aaagaatgtt tttctctcaa tttttttgac gtttctatca tgaatcacga
      121 gaaaacttgg gacactttta acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc
      181 ttggttcttt tcttgtttca attcgatata cattcctaga gaattttttg tcgtatcttt
      241 ccaagaaaga ttcaacttct tggcattttt tacaatcatt tcttgctaga tacacaaaaa
      301 atgattgatt cgttttttga agttctagta cttcttctaa attactattg attagttttc
      361 catttgattc ttcgttagtt tttatcagtt taaaacaacc aaataagagg aaactaacaa
      421 taagtatcct aacaataact ttcttatttc gcatattgtt tctccaaatt tggtttccga
      481 atatagacga ctcgcggtgg ttcctggacg tcttcagctg ttcgatacac cacaaattcg
      541 cctgttctca atgctcgaat gcgattcgga ttgaatttaa agcggtctac gtctcgtttc
      601 gtgcctcttt ctcctgtcca atcaaccttt cccatatcag aataacctgc ttcttgtgtc
      661 gtcatttccg ttaattcaat gtcttcttct gtccccatta aatctgccca ataatcaatt
      721 tctgtcgggt catttgtctt cccaataaag aacgtattca cattcccaat caactgtttt
      781 cgaaggacag gatcaattct atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc
      841 accccagcgc ctcgtccctt attgactaag tctataaggt tatcatttag ataagaagct
      901 ggttcgtcaa atattactag gactttcgag cgattggctg tttctcctgc ttgtttatac
      961 acttctgacg caaagaaatt aatatctccc acaagcattt gagccaatgt ccgaataaac
     1021 tcactataaa tcaatccgtt taacgagata taaacaatct ttccttcttt aacaacttct
     1081 tgaatatcta attctctttt tcctccagaa gtatcaaaga gttcaccaag ctcactatcc
     1141 aataacatat tgatattggt ctgtaaattc tcagaagtcg catacaaata aaacgggttc
     1201 ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg
     1261 agagaaatgt tttcttttaa taatagataa tacgtttcta agtcaacggt tttacgatca
     1321 tgttcaacga ttcgctcttc acttaactgt tccacatcgg caaaatcacg ttctgttttg
     1381 tccatcgctt cattcttttt cgtttttttc ttcgtggttt tcttcttttc tacgaccact
     1441 ttttctttaa ataaatctgg atttttcaac tgaatacgat ccgcaaacaa atgtaagacg
     1501 tttcgtggca ataaataatg ttgtaaccaa actagatttc tttcaatttt atggctcttt
     1561 tggaactcgt ctaaaagctg aatcgttacc tgtaataacg ctttggctgc tgtcgaataa
     1621 tacacgcttt ctgtttctgc tagattggtt aatttatctc gaatggcaat atcattgcca
     1681 ttttttacag gattataacg caaatcgcca ccgtccacaa agacttcaat cgggcgattg
     1741 tatgcctctg ccacttcttt taacgcttgt aaagtctctc ctgctccttt cccatcgact
     1801 aagataacgg ggatacgaaa cttacacgca tgttctgcta gtaattggat caccgtcgtt
     1861 tttcctgaac ctgtagtcgc tggaataatc acatgttggt tcaattcact aaagttcatc
     1921 acataaggga gcccatactc atctgttcct aatagaatca ctttggctaa tgaagccagt
     1981 tgtttattcc gcaaagcaac gtcttttatg acaattgcta cctggtacgc ttgctgatac
     2041 tctgctgtat atttttggga ctcctgaaat ttattcttcc gacgttcctt gtattcttta
     2101 ctatttagca aagctgtttg ccgttctttc tttgaaacaa cttttctttt ttcaataaaa
     2161 gacgcatacg caatcaaaca acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc
     2221 gtttgtccat taaactgcaa cgcatgagaa aacaagttta atggaaataa taagtaacac
     2281 gacaaatacc ctctaggaag aacaaaaata aaacaaaata gatttaatag cgtaaaacct
     2341 aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga agctctcata ctcaaagtca
     2401 taaatatgtg cacgtgtctc ttttttatct atcataaaga caatcgctaa aggaatactg
     2461 cctacaataa aaaatggata tagacaaaca cctaataaag tgacgataaa ataaagttgt
     2521 tgaaaccgac tttttggcgt ttctttttct tgttttcttt tgcgatcgac agaaaacatt
     2581 tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac tatcgatttc ctcctatcgt
     2641 tttcgttgtt tcatttctct tcgataaatc ggttgttttt ctcgttttcg tttcattttt
     2701 gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt gttttggtga ttttgtttgt
     2761 ttcatggttg agtatgcttt agatacggtt gaaggcgctt tggtttgtgc tgttactcga
     2821 ttgactgctg acatatgttc ccgaaccccc tctggacgtc gaacagattt cggtgtggta
     2881 gaacctgttg ctcgtggtac attccctttt cttagtggtt tttgtaccgc atgtttttgt
     2941 cctgcaactg tccgggtttt catcgaactt gtttttgtta cgggagtaag tgcctccttc
     3001 cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta
     3061 tgcttctggc cataagactt gtgtttttgc tttggattcg ctaatgacac caccattgca
     3121 gaaccctgat tgcgatacgg actacgagac gcaccttttc tacttctttc tcttagacta
     3181 ggattgcctt gcaattctcg ttgtaataaa tcggctgttt ttcttgcctg tttctccatt
     3241 gctgttcgtt gcatacgttc ttgactttgc tcttctgctt gtttcgcttg atcatgcaag
     3301 tctcttgctt tttgtgccat acgttccata cgatttggtg ctttatgacg aggttgttgt
     3361 gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg
     3421 tctgagccac ccccacgttt tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct
     3481 ttttgtttct gtgcttttct cgcttctttg cgttctttca accgttgttt ggcttccctt
     3541 ctcaattttg cttctgcccc aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc
     3601 attcttccag taaatagact gcctaaaaag cctcggaaca aataaattcc caacggcgca
     3661 aacaaataaa caatgatcaa gagaattttt tcccatggat cgtcttgtgt actacttaat
     3721 tggaacccgt aactaagaaa agaagaaaaa aggatacatg caaaacctgt cattcctttt
     3781 aaaaacgtca aaataccaaa aactttggca tagttaccaa acacattcat tgtgacaaga
     3841 aacaatccta aaaaaagtaa gaacggcaac atcggtacta acaatagttg catagttcca
     3901 ataatcaatc gaaacatact taagacaaaa taaacaacgg tttgaatcag attcacgaca
     3961 aaataaaaca aggcaaatgc tgtatttttc caactattgt aatattgtat gttcttgttt
     4021 ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa ttttctcccc tatttttgta
     4081 cctaaatcat tgtccaataa aagattgatt cgatcatatt cttttccagc atatttgact
     4141 ttcttctcac gaattgtgtc tacattggtt gtgccataat ttaacaacag atatggttca
     4201 taaacattac tataaaagac ccgagccgcc atcaatttgc ctgccgtttc cactttttta
     4261 tccgctgtta cctcttctgt ttcttttgtt tcataatttg tttcttcttc aggtgttaac
     4321 actggattga ccttcacgaa cgccgtttcc actgtagaat ctaaagaaaa aagaacatca
     4381 aaaagattct tatttgtccc agaatctttt aatacgctta acccagtaaa aataaggatc
     4441 gtcataagaa agacacggag tgcttgtttc attcgagcct ctccagcata tttgattgcc
     4501 atcataattg ccgaaaaact gattccaata gttcctgcaa tacttaacat attgtccgca
     4561 attttttgtg ttaagttttg aatgggttct cgaatggcgg aaataatatc taattcaaag
     4621 agaaattcaa ccattgaagc attgaattct cctaacattt ttacgccact ccaaacaaaa
     4681 tttttcatca ctcctgcaac attgtacgat agcttttttt taaggttcat tgactcatag
     4741 tcattggtca tcaattcaaa cgaattcttt ttgtaatttt tatacaattg atccacatca
     4801 gtcgttactt cttttacttc tactttatcg ttggcattct ctccttcaga acgatcggtt
     4861 tcagtgacac ccgcaccatc attcatttga tcggcagaaa cagtagcccc ttgtagacag
     4921 aaaaaaagac cccctaataa aaggagcgtc caccatttca gagatttttt tcgcaaaaaa
     4981 accacctctt ttttgtttta tcgttgtttt tcattttcta acattgcttg aattttttca
     5041 tcttgatttt cagtagaaga aacagccaat aaccaactat caaacaacac atcaatggca
     5101 atcggttgat ttcttcccaa atgatcctgg aataaacagg ttcctgtttg taattgttcc
     5161 atcatttcta aattggctga attcacttcc atatcaaaga aggacaatag tttctctcgt
     5221 gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct
     5281 tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct
     5341 tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg tatcttcata cccttttgcc
     5401 tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag aaaagatata cgtaaacttc
     5461 ataatgactt ccataattgc cattcctgca agttgttcgc tatttaatcg acccgaatca
     5521 atctcttttt gtgtcggaat gcgtattcct tgtgtcccta acacgttgat ttgtgaatca
     5581 aaacgtatcg gcgtactgtg ctcattccca atgagaatct ttcctaaacc aacgctatgc
     5641 cctaaaatca atttagctag ttctttgtca ctttttcgaa tttcttcgat aacttgactt
     5701 aacgaatgct tacctggcat ggtgtaaact acatcgatgg cgtcttggat caccgttttc
     5761 ttgtggctcg ctgtttctcg attggtatct acttctccta acattcctaa cacattttta
     5821 gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag gatcaagcat ccctcgacag
     5881 ctttcatctg aagataacga aataaaatta attcgttcat acatagccac aaactctggt
     5941 atgtgtccga atttttcaag tgcctttaaa aagaactttt gggtttcatt tttcggatca
     6001 atataaagaa ttttttgacc taaaaacatc agccataaaa aaatatattt tacaaggacc
     6061 gattttcctt tacctggtgg accaataatc aacgtatttc cattggtatg ttccgcgcct
     6121 tcaatacttt tctttgttaa attgggataa taccatacta gtttactaga tagcgctaac
     6181 gcttctttcg cagatttaaa cttttttgca ataacaatcc gtcctaaagg aaatcctttc
     6241 ttatttccta cttctttctt taagtccaag ccataatctg ccacaaaacc tgtcgtagcg
     6301 acctgctcgt aactacgaaa acgttgcgta cttccaatta agcattgatt aaacaacgtt
     6361 agctgatccg ctaccgatcg ataaaatttg aaatctgtag actcaatcgc aaattcaata
     6421 tctttgactc gttcttccaa ttcttctttt gaagaagcag atacaaccag cgtgacggta
     6481 gaataacaca atcgtttttc tttgcgcttc agcttatctt ttacatccat taaccgttct
     6541 gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt caacggtctc atattctttt
     6601 tcttgttcat aaatccgtcc agccatttta ttaatttttc gttcatcaga ttttgaatgc
     6661 tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata agctatcctg aagattttga
     6721 ataaagctac tcccatacat ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa
     6781 tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt cattcgaaat gacgccctcg
     6841 caaatttcca ttggcgtaag ctgtctttca ggcaattttt cttctgatcg atggaaaaaa
     6901 taataataaa tttgtccaat ctcttgggca gataagcgct ctatcggctt ataattggac
     6961 aattccgtat agagctcctt ttctaatgtt tcatacgttg gtaacacact acttatagga
     7021 actcgttgtc ccgtaatacg atagataaac tttttaaaaa catgcttaag caattgaaaa
     7081 tattcgatcg gatcagacac tttcatttct gtagtgaaac gcacaaacag atacgttcga
     7141 tactcgttta attgcacttc gtcttttaaa atctttcctg cctgttcaaa gtacactttc
     7201 ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct
     7261 aggttaaatt gacgtggaat attgataaaa tgatagtcat attcatcatg tccaagtatg
     7321 ccttcgccat cttgaagata gttcttgaaa aaatcaatgt catttaaagg aaatacttgg
     7381 gaagaaagtt gatagcctac ccacacatca tcatttttgg tcaaaacaaa atttcggata
     7441 atggcgtcta tcggaaattc taatcgagga agcttcgttt ttttcttttt gaactgccaa
     7501 atcccgataa taaagacgac caaaagaaac aaaaacgcca tttactcgac tcactccttc
     7561 catttgtgta gggaatacgt cactttttca tggaaaaacg gcacatacat ttcatgttca
     7621 aagcgttgtt tccgtttctg ataataggac gttcgcccaa ttaaatactc gaaaaagtgt
     7681 ttattgtccc atttcattga aaacaatgtc caaacgagaa caccaatccc aaaaatcata
     7741 atcaaccagg actttcgaaa cagtgtctgc aagaaactaa tttgattcac atacgcaatc
     7801 aaaaatagga tcaaaagaat gaagagaaat accccaccta tgactaattg ttccatgcga
     7861 atgccattag aaatccaggg aattttttgt cctttaatcc cgtaaatttt gtgagggtgc
     7921 ttaaactcct ttgtatagtt gtattccatg ttcactctac ctccttttac aaaaaaagaa
     7981 gcgaccagaa gttctaatct tctgatcgct tttgcagttt gtctttttct tatgataatt
     8041 gtatccaggg cacaatcatt tgcgttaccc ccacgttctc ttcactatcc ttaataaaga
     8101 taatgttctt tagccattca ccaatatcaa cggcattcga taacaataag acgagcatta
     8161 tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag
     8221 caccaaccat tagaacaatc gcaacgattg ccaatagcca aatgacttgt ccagatacat
     8281 catcaaataa cgatttaaga tcaaaaagtt tcatttccat tctcctctaa gttcatttta
     8341 ttcatttaac gtagtaacaa aataactatc gccacttgtt tttacttcca ttgaaaagtt
     8401 tgattggaac tcgtttcctt tctcaaaata gaaacgatac ttcccttgca aaaagtagtt
     8461 tccttcttta ttttttactg catgatccag ttgtacattg atcaattcgc cgccttctaa
     8521 ccctttttcg ttagaaatca atgccaattt ttcatcattt ttgacatata actcaaaaaa
     8581 ccgtttcaca aatgcttcca gcttgatttc ttcgctttca gtgaccgctt gcccagaaga
     8641 ataaaatcga ttttcctgat agttcacaga attggtattc ttactctcag aaagattcaa
     8701 aaattgaacg ccatcaagta cttttaaaac attgttttga taactgactg gaaaagaaag
     8761 ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc tcataaaata ggcgatacat
     8821 tgcttgtgtc aaatcgattt cttctagttt aataaagcga acatctttcg ctactcgatt
     8881 ggtagccgtc gttacaaagc ctagatcaga cgcttccaat ccttgtgcta aataatgatt
     8941 gacggaagcc tcccattcgt ttcgtttttc tggcgaataa gtaaataacc tttcaataaa
     9001 atgttgtccc tcgtacttca attgttccgt agaagacaag gtttggttga cttcagttaa
     9061 caatgtttct ggattgactt gttttttcac tgctaaacgc gttaatgtat ccactcgtcc
     9121 aaagtttagg aggagcacga cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa
     9181 tacactcatg gcactttttt gcgaaaattg gatcattcga tctgacggtt ctgttttctt
     9241 tttttggaac aaaaaacgtt tcttttgttt cttttccttc tgttgtttcg gtggcttagt
     9301 ttggaacgct tgtggattaa attttgcatt actcatgaga aatgccatac cgttctaaca
     9361 ataatttcgg tattttgtcc acaatcacta cttcgtggac aaccacactt gccccctc
//
LOCUS       DACDCV010000100         8934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDCV010000100 DACDCV010000000
VERSION     DACDCV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.100"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..1822)
                     /locus_tag="IV041_002392"
     CDS             complement(50..1822)
                     /locus_tag="IV041_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8383143.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1776..3530)
                     /locus_tag="IV041_002393"
     CDS             complement(1776..3530)
                     /locus_tag="IV041_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8383144.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3640..4209)
                     /locus_tag="IV041_002394"
     CDS             complement(3640..4209)
                     /locus_tag="IV041_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8383145.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4227..5651)
                     /locus_tag="IV041_002395"
     CDS             complement(4227..5651)
                     /locus_tag="IV041_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8383146.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5653..6321)
                     /locus_tag="IV041_002396"
     CDS             complement(5653..6321)
                     /locus_tag="IV041_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8383147.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6451..7035)
                     /locus_tag="IV041_002397"
     CDS             complement(6451..7035)
                     /locus_tag="IV041_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8383148.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7366..7599)
                     /locus_tag="IV041_002398"
     CDS             complement(7366..7599)
                     /locus_tag="IV041_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8383149.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7614..8564)
                     /locus_tag="IV041_002399"
     CDS             complement(7614..8564)
                     /locus_tag="IV041_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8383150.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8561..>8934)
                     /locus_tag="IV041_002400"
     CDS             complement(8561..>8934)
                     /locus_tag="IV041_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8383151.1"
                     /translation="LITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILI
                     VAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFL
                     FVISFLISPKQKFLRKKERSL"
BASE COUNT         2981 a         1638 c         1182 g         3133 t
ORIGIN      
        1 aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat catcgtgatt
       61 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
      121 atatttctga ccttcgatac aactcattat gagtaccttg atcaactagt tttccttgat
      181 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      241 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      301 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      361 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      421 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa cgagctgctt
      481 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      541 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      601 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      661 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      721 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      781 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccctctt
      841 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      901 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      961 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
     1021 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1081 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1141 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1201 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1261 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1321 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1381 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1441 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1501 cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta tataagtttt
     1561 catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat acggcaaaat
     1621 aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1681 atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct ttagttaaag
     1741 cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc attgtcaagc
     1801 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1861 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1921 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1981 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     2041 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2101 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2161 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2221 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2281 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2341 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2401 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttctaaa
     2461 actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac attttcaaat
     2521 gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt
     2581 tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa gtgcatactt
     2641 acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa caacatcaac
     2701 aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt tataggaact
     2761 atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca
     2821 ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt caaggcttta
     2881 aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttccgcggat atattctacc
     2941 gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt tgtattctct
     3001 gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac caatccaatt
     3061 ttaaagttaa tgaaaaagcc taaggcaact gataaaacag gtgttactat ggcttgagca
     3121 ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc atcaattgtc
     3181 tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg
     3241 ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg agaaaataag
     3301 ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg aaccgcataa
     3361 ctcttagcta aatattcatc tcctacaata attagagact gaagaaattt gtaaatactg
     3421 taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc taaccatccg
     3481 tagtatttca ttttagggac ataactaaat aattttttgt atactttcat tttttcctcc
     3541 cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg taatagcgta
     3601 aattagagat gtacaaacag ataaaaggta aactacgttc tattttttgt atttttttaa
     3661 gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta acactgaaat
     3721 gattccccat atacctaccg catagtggat cattgcatcc attgttgcag cagattgtcc
     3781 tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa tccactgtcc
     3841 ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg ctcgatcata
     3901 tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac tgataaacca
     3961 aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt
     4021 aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc cagataataa
     4081 tccaaaaatc ccgggggata tgctgtgacc aaacgcccca ccaatactac taaccattat
     4141 tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa tataatcttt
     4201 tgttttcata tatattctcc tcaattctat ttcgttatgt cattaaaaat ctgttctatc
     4261 aaagttgaat cttctactct aagttttata tcttttttta ttccaccatt ttttaaataa
     4321 ataattctat tgcatgtttt ataaagaaat tccggatcat gagtagctat aactactgca
     4381 cccttttctt ttaagtcttt aataaggtca gcaatccttt ccatattaat ataatccaac
     4441 ccacttgtag gttcatcaaa tagaaataaa ttagtttttg acaatgaagc tatagaaacc
     4501 aataatcttt gtttttctcc accggataaa tcaaacggta attgatcttt cattttataa
     4561 agttccattt tttttaaaat tttttcttgc tgatctttac taactatttc attccccagg
     4621 gctaactcgc tcctcactga ttctgtgaaa aattggtaat ccatttcttg cattactaaa
     4681 aacggcgatg acaaattttt tttattttcg attatctttc ctttggtagg gcgaataatc
     4741 ttcatgagta ttcttagtaa ggttgttttc cccacaccgt ttttccctac caaaccaata
     4801 acatctccaa ctttgacatc aaaatttatg tcttttagaa tattttcata agtaatttct
     4861 tcaactttta atacatctgc acttaccttt tcgatatagg gaacttcaat cgaactatta
     4921 aaaatgtcaa atttccttaa tgcgttattg ttttcagttt ttaacaactg aatagttgta
     4981 gtttttacag ttctattttt aacaaataga acttgatcaa ttaattcttt caaatagtaa
     5041 aaacgatgtt cagctataac aatagtaaat cctttatttt ttaaatcaca aagtaagtca
     5101 gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa tataattaat
     5161 ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc acttgatagc
     5221 gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa ttcattgatt
     5281 cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac taactcactc
     5341 gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc aatatttttt
     5401 gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat ttccccaacc
     5461 aattttccgt cataaagtct tggaataagt ccattcatta actttaatag tgtggatttc
     5521 ccactcccag aagaaccagt aatcaatgta agactgcctt tttttattga aaaattaagg
     5581 tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt aaattcaaac
     5641 aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa acaggatgat
     5701 atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag gagattctat
     5761 cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac gtataagaac
     5821 gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc gaatgtttct
     5881 catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa agaatctgat
     5941 cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta aagaggataa
     6001 taattcattg ctagtataga cactaaccaa caaccatgag atcattacga ccggcaacat
     6061 tcgaaataca atatataagg atgtcgcaaa aaatatagta aaaaactcta tcttgatagc
     6121 atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat aaatacatgt
     6181 ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc ccacttgaaa
     6241 tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag taatcaaggt
     6301 taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac tgttatctta
     6361 agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa agcataactg
     6421 agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc acccaggaac
     6481 agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat agctatgctt
     6541 caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt cttcttcatt
     6601 aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac aacagactgt
     6661 ctttttcgtc acaaatgatg taattttttc aacaatatct tgatatttgg ttccagctga
     6721 ttggaataaa atcaaatcga atatacgttt attctcaaaa atataattaa taaatggatc
     6781 taattcattg aagttctctc tccaactttt ctctatatct atattttcta agtttaagag
     6841 gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg gctgaataat
     6901 ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg tagttacgcc
     6961 tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt caaaatggct
     7021 tagtccactt tctattaact tttggtgagt tataatataa tctctggttt ctatgggtac
     7081 ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag tttcaatcgt
     7141 taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag cagaataact
     7201 gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca ccttttctaa
     7261 aataatccga gaaagattta aaaaaattat atccagctat taggataagt cctatcataa
     7321 tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact taaaaccttt
     7381 tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta tatctaaata
     7441 gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa cacttagaac
     7501 ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct gaattgctag
     7561 taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc aaattatttc
     7621 gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta actatcacca
     7681 tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg aatattggta
     7741 tcttttgata ctgttttcat tggtctatca tccacactgc tttctacgaa taaggaggga
     7801 acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt tccttcttct
     7861 tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga gaaatattta
     7921 aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc aaatttagat
     7981 ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt ttctttatag
     8041 aattttttat tatcaggatc tttttccgct aactgttttt caatattttt agcgtaaata
     8101 ataccgtttt ctaaatttaa ccaagcatga ggatcttcct tccctttctc actctgaccc
     8161 tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt ttcctctttg
     8221 ttcgcatttt taactaattt tgtaaaccaa gcatttcctc cagtttccaa gttaacaccg
     8281 ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa aggttcatat
     8341 tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc ttttgctata
     8401 ttttcggtaa tatctgctaa aatcgaatta gtaactacta cttttaattt ttcgttcgaa
     8461 gccactttat ctttgtcttt actatttatt gaacatgccc cgaatataaa aagagcaatc
     8521 aaaatcccca gacttcccag tactgctttc cattttctca tcataacgat ctctccttct
     8581 ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag aatgccgctg
     8641 ttaaaacaat acttgatcct gctgctatgt taaaactata accaataaac aatccaagca
     8701 atgatgaact tgctccgact gttgcagaaa taaatatcat agttttcaag ctatttgcat
     8761 ataaatatgc tgtagctgct ggtgtaatta acattgcaac aattaatata gttcccacac
     8821 tttgcatagc tgttactgct actaaagtta atagaaccat caatagataa tgataaaaac
     8881 tgacattcat ccctattgct tttgccatta atggatcaaa agaagtgatt aata
//
LOCUS       DACDCV010000101         8921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDCV010000101 DACDCV010000000
VERSION     DACDCV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.101"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="IV041_002401"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="IV041_002401"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8383152.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1713..3059)
                     /gene="gor"
                     /locus_tag="IV041_002402"
     CDS             complement(1713..3059)
                     /gene="gor"
                     /locus_tag="IV041_002402"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8383153.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3193..4434)
                     /locus_tag="IV041_002403"
     CDS             complement(3193..4434)
                     /locus_tag="IV041_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8383154.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4452..5375)
                     /locus_tag="IV041_002404"
     CDS             complement(4452..5375)
                     /locus_tag="IV041_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8383155.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5596..8088)
                     /locus_tag="IV041_002405"
     CDS             complement(5596..8088)
                     /locus_tag="IV041_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8383156.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8095..8562)
                     /locus_tag="IV041_002406"
     CDS             complement(8095..8562)
                     /locus_tag="IV041_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8383157.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8698..8771)
                     /locus_tag="IV041_002407"
     tRNA            complement(8698..8771)
                     /locus_tag="IV041_002407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8735..8737),aa:Arg,seq:acg)
     gene            complement(8829..8902)
                     /locus_tag="IV041_002408"
     tRNA            complement(8829..8902)
                     /locus_tag="IV041_002408"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3073 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     1081 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     1141 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     1201 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     1261 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     1321 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     1381 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     1441 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     1501 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     1561 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     1621 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     1681 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     1741 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     1801 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     1861 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     1921 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     1981 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     2041 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     2101 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     2161 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
     2221 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
     2281 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
     2341 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
     2401 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     2461 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     2521 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     2581 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     2641 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     2701 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     2761 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     2821 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     2881 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     2941 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     3001 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     3061 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     3121 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     3181 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     3241 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     3301 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     3361 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     3421 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     3481 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     3541 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     3601 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     3661 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     3721 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     3781 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     3841 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     3901 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     3961 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     4021 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     4081 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     4141 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     4201 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     4261 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     4321 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     4381 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     4441 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     4501 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     4561 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     4621 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     4681 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     4741 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     4801 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     4861 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     4921 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     4981 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     5041 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     5101 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     5161 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     5221 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     5281 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     5341 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     5401 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     5461 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     5521 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     5581 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     5641 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     5701 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     5761 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     5821 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     5881 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     5941 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     6001 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     6061 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     6121 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     6181 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     6241 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     6301 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     6361 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     6421 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     6481 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     6541 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     6601 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     6661 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     6721 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     6781 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     6841 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     6901 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     6961 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     7021 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     7081 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     7141 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     7201 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     7261 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     7321 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     7381 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     7441 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     7501 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     7561 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     7621 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
     7681 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
     7741 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
     7801 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
     7861 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
     7921 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
     7981 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
     8041 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
     8101 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
     8161 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
     8221 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
     8281 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
     8341 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
     8401 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
     8461 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
     8521 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
     8581 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
     8641 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
     8701 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
     8761 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
     8821 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
     8881 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DACDCV010000102         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDCV010000102 DACDCV010000000
VERSION     DACDCV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.102"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IV041_002409"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IV041_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IV041_002410"
     CDS             complement(1456..1791)
                     /locus_tag="IV041_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8383158.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IV041_002411"
     CDS             complement(1775..2047)
                     /locus_tag="IV041_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8383159.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IV041_002412"
     CDS             complement(2498..3301)
                     /locus_tag="IV041_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8383160.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IV041_002413"
     CDS             complement(3279..4403)
                     /locus_tag="IV041_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8383161.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IV041_002414"
     CDS             complement(4419..5234)
                     /locus_tag="IV041_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8383162.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IV041_002415"
     CDS             complement(5224..6105)
                     /locus_tag="IV041_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8383163.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IV041_002416"
     CDS             complement(6181..7470)
                     /locus_tag="IV041_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8383164.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IV041_002417"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IV041_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDCV010000103         8839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDCV010000103 DACDCV010000000
VERSION     DACDCV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.103"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IV041_002418"
     CDS             278..787
                     /locus_tag="IV041_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8383165.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IV041_002419"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IV041_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8383166.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IV041_002420"
     CDS             2322..3302
                     /locus_tag="IV041_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8383167.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IV041_002421"
     CDS             3424..4842
                     /locus_tag="IV041_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8383168.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IV041_002422"
     CDS             4913..6382
                     /locus_tag="IV041_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8383169.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IV041_002423"
     CDS             6392..6859
                     /locus_tag="IV041_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8383170.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IV041_002424"
     CDS             6895..7767
                     /locus_tag="IV041_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8383171.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8839
                     /locus_tag="IV041_002425"
     CDS             7848..>8839
                     /locus_tag="IV041_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8383172.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSG"
BASE COUNT         3039 a         1320 c         1783 g         2697 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcgg
//
LOCUS       DACDCV010000104         8753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDCV010000104 DACDCV010000000
VERSION     DACDCV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.104"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..827)
                     /locus_tag="IV041_002426"
     CDS             complement(<1..827)
                     /locus_tag="IV041_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8383173.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQT"
     gene            complement(833..1939)
                     /locus_tag="IV041_002427"
     CDS             complement(833..1939)
                     /locus_tag="IV041_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8383174.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1939..3075)
                     /locus_tag="IV041_002428"
     CDS             complement(1939..3075)
                     /locus_tag="IV041_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8383175.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3107..4558)
                     /locus_tag="IV041_002429"
     CDS             complement(3107..4558)
                     /locus_tag="IV041_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8383176.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4602..5366)
                     /locus_tag="IV041_002430"
     CDS             complement(4602..5366)
                     /locus_tag="IV041_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8383177.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5394..6092)
                     /locus_tag="IV041_002431"
     CDS             complement(5394..6092)
                     /locus_tag="IV041_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8383178.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6104..6883)
                     /locus_tag="IV041_002432"
     CDS             complement(6104..6883)
                     /locus_tag="IV041_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8383179.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6899..7843)
                     /locus_tag="IV041_002433"
     CDS             complement(6899..7843)
                     /locus_tag="IV041_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8383180.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7889..8668)
                     /locus_tag="IV041_002434"
     CDS             complement(7889..8668)
                     /locus_tag="IV041_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383181.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2472 a         1833 c         1305 g         3143 t
ORIGIN      
        1 aatgtctgtg ctatttcatg atttattctc tgtgctaaaa caatcatata atctatcatc
       61 aaataatcca ccatcatatc attatttttc caataattta aaaaaaagtc tctaattata
      121 gcaaacatcc atctagactc ttgattacat gctaatgaat aacccgcaaa atatcctgat
      181 gctacggatg cgtgagcata atcaggcctc ctaatagacc aaatatcgat atcaaaatac
      241 tcatctggta ttggttttgt acaataaaat gtggcgtcta accacaaacc tccatgtttt
      301 gctagcaaag ataatcttaa taaatcagaa taatttgttc tcgtaatgat tcctttgttt
      361 ttcttttctt ccacccattt tggtatatca acatattgtc tataattatc ttctgttaaa
      421 attataacag tatgtttccc tgcatttcgt tttattgatt ttacacaagc ttttacaatt
      481 tccggcgatt gatccaatcc ctgccaccaa caaacccaaa tgcagttaga atatggtgat
      541 tttgattgat taattatttg attttgacta taatcatatg ttttcaaaaa atctgaaaaa
      601 gtcttttcat aatacttcag cattacctca tgttttttta ataaatgttc cttctgtttt
      661 ttactttcat tatatccatt acgattcatg acttgaatat caattttagc ttgtaacgtg
      721 gcaaatgctt ccttccaaga tgttatcttt gcaattcgtc ttgttgcatt caattcttcg
      781 gatatgcgat gtataattct ttttatactt gaatgtttct gtgtcatatt ctttaacctc
      841 tttgtattaa ttcaaataac tcataatatt ttgattctaa gattttgtat gctgtttgaa
      901 tattataatc tgcctttatt aaaatcgaac gttctgaatc tcgatgagag atatctacat
      961 ttgcaatctc tctcgcccat tcttttgccg attgatttaa acttatgaaa ttggtcttat
     1021 cactgaaagc aacttctgta ggaactttct ccgaaaagaa acacggtaaa tctgcaaatt
     1081 gagcttcaat acccactaac ggaacccctt caaataatga tggcaacatt aatacgtcaa
     1141 atgcttgata aaatttttct atatcatttc tatttcctaa aaatttaata tcatcatcta
     1201 tgccactttt ctttgcataa ttttttattt cttcttccat atcaccagtt ccaataatta
     1261 ataatacgga attattctta atagcgtgaa attctttaaa aatttcaatc aaaaaacgat
     1321 gatttttaga atatgaaatt cttccaatat gtcccattac aaacgtctga cttgaaattc
     1381 ctaatttcat tcgtacatca tttctaatac tttcatcata ataaaatttt ttagcatcca
     1441 ctgcatttgg aatcaaagtg aatttatcat ttcgaaacaa aaaatcacct gctacttttc
     1501 cacatgaaaa ttcatgtgtc aaaacagacg ttaacttaca tcgataatat tgctttaaaa
     1561 gatatttaaa atctctatct atcgatgtat tatggctatg tgctatccgt atcggaatcc
     1621 cagctttttt tgcagctttt aaaggtaaat aactcatcgc atcaatatga gaatgaacaa
     1681 tctcatattc tggatgatcc ttaaaaaatt gttccatcca tttaaaatat ttaggataat
     1741 tttgtggata aagtctcggt gcataataga cctttcctcc aagacttatt atttcctcat
     1801 cataatcact tttatatgga cgatgtgtca aaaaatcaaa ctgaatttta gttcgatcta
     1861 tattcctata atagttcatt agcatcgtct ccaaccctgc acgatgcata tcattcacac
     1921 actgtaatat tcgaatcatt atatatttcc cctatacaaa tttaatgttt cttgtacgat
     1981 tttatcccaa ttatatttac tacaaataaa ctcagtagct tgattcttca aaactttaac
     2041 catttccgat tgattgcatg cctcttctaa tcttatcttt aaatcactta catccgattt
     2101 cttaaaaatc atagccttgt cttctactac ttccgtacac tctgagatat ttgaaactat
     2161 acaacagttc ccataactca ttgcttctaa caaacttaat ggcattcctt ctaaatcact
     2221 tggaagtgta tagatatacg cattactata taattcttct aattcctgtc cttgtacaaa
     2281 tccagtaaat ataattcgct catcaccttt tgctaattcc tttagttcat ttgcaaattc
     2341 atctgtatca gagcttcctc cggctattat aagtttttta tctgtctgaa catctttaaa
     2401 tgcctcaatt agataccgaa ttcctttttc gggaaccaat cgccccaaaa acaaaatata
     2461 agaatctttt gttaatccat acttttctgt aatcaatcct gctgatttta tttcaatttt
     2521 tttaacgcca tttggtataa accttgtttt tcttccatat atatcttcaa aataattttg
     2581 gacgctttca ctaagtacaa ttatttcatc tgcaaatttt acagcacact tttcccctaa
     2641 catgatgtat gtactagcaa gcttgttcca tttttctctt tgatgatcta gaccatgaat
     2701 tgttgcaaca catcttttcc caaaaagttt tggcaaccac aacatagcac atggtccttc
     2761 tgcatgaaaa tgaacaacat catattttcc gaatgctgct ctaattccac caaatactga
     2821 agaactcatt gctgcaattc cttttatatt taatgtaaaa atagtcttta attttacacc
     2881 tttatattct ttatttttat gctggtcaaa ttcctttccg ctaacatgat gaccacttcg
     2941 attatagcaa gtcacttcat gcccaagtgc aaccattcgc gttgccaatt cttctaccac
     3001 tatctcaatt ccaccttctc ttgatggaat tcttttatga cctagcatac agatttttaa
     3061 tttttttctt gccatcacgt tttactcctt ctatagtatc tttatcttac atggaaccgt
     3121 ttctatgaag cacagttttg actgtcctaa acaaaattct aaaatctaat cccatactcc
     3181 aattcctgat atattctgca tcaagtttta caacctcttc gaagtctgtg atatcacttc
     3241 taccactcac ctgccacatt ccagtaatcc caggtttcat cgcaattctc gcacgatgat
     3301 gaaaatcata cttctcatat tctgacttca agcaaggtct aaaccccacc aggctcattt
     3361 ttcccattag gacattataa aattgtggaa actcatccaa tgatgtagat cgaataaatt
     3421 gtccaattcc tgtcttcttt gttccatcag gaagtatttt attgccaatg attcttgggt
     3481 caaaatccat cttaaacatg agattattac tcatcttatt ttctttcatc aactctgctt
     3541 tgcgttcttc tgcatccata tacatactac ggaacttata catcttaaat gtcttaccat
     3601 tttgaccaat acgttcttgt gcaaagaaaa tcggccctgg tgatttgatg taaataactg
     3661 gtgctacgaa gataaaaata acacctgttg caatacatcc aataattcca gcaagaatgt
     3721 caatcgttct ctttataaat gcttgcctca tcgtcataac attaatacta gatgtaataa
     3781 cagtataatc accaactcgg ccaataaatt gttttccacg tgtactatca gacacttttg
     3841 taagattcat atgtacagca agtcccattt ccgaacatct ggcgattaat tctttgggat
     3901 atggaacact ttcgtcaaca ttgatgaata cttcatctac ccaattctgt aaaagatatt
     3961 ccgctgcatt atcagcattt gcaactactg ggacacctcc gatggtagtt ccatacatat
     4021 ctctgtctac tacaatcaca ccactaagtt tatacatgcc ataattcttc tctttaagat
     4081 tacgaactac attttttgca atcttttgag atgttacaat aactaacgat acatcttcac
     4141 cgtttttcat gcggtgatgc aacaaccctt tccaaagtat acgaattata tatgttaata
     4201 atccatacac cacccccatt ataaataaag aaactctgga ataatcattt ccatcctgca
     4261 ttgtaataag gtataaactg cacacaagta acagcataaa agattgcttt atagaagcta
     4321 cagcctcttt ataatatcct ctttttaaaa catttttata tgcctcaaat aaaaatataa
     4381 tcgtaagatt ctcaagttct ataaaaattg ccatgtggcg ataaatagga atcacatacg
     4441 gattccagtc accctgtcgc attacatatg atatataaaa agcaagctgg acacaaaata
     4501 agtctaataa aataaaatca atatgtttag accaaccact tgagtttttt ctatacatct
     4561 ctcactctct cctcatttcc tttttatcta cgaataaaga actaaaataa accaaacttc
     4621 ttctttttta cctcttcata gattggataa ttgatctcat ccccgttgat cagatccttt
     4681 gcgacttgtt tcatctgtac tactttttcc ttaccaaact ccttggcaat ctgctcatag
     4741 cactctttta agtaaaaggt gcgcttagag accccgtgtg catcagaagc agccatctgc
     4801 acgagattat gtttgaccat tgttttggct gttttctgga tatctttgcc aaatacacca
     4861 acataacttg gcgcagtaag ttgggccaaa catcccatat ctaggaacgg aagcaaatga
     4921 tttgggtctt ctcgaaaatg cgcgttgcgt tctggatgga cgatcactgg tatctttcct
     4981 agttctaaca actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat
     5041 tcgatcagaa gatatgtatc atctaaatct gtaaataaaa tttcatctcg gtggatgtct
     5101 tcgatgagcg ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt
     5161 cttttctcta attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg
     5221 ctgtattttc cattattgtg gtgtggtgtg cataaaatat gcgtaatgcc ttgctgaatc
     5281 gctttttcag ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt
     5341 aggatatggc aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt
     5401 ccgtagtaat aaccagcatc tttgctgtgt tctgctccgt tgtatacgac acctaagact
     5461 cgtgcttgta ccatatctaa cagttctttt gctttagtca atgattcttt tcgtgacgta
     5521 ttttcccgta cgaccaataa ggtgccatcc gtttttgaag acatgatctg tgcatctgtt
     5581 acggcaacga ctggcggcat gtcgaagatc actacatcaa atagttgtct tgcttctgct
     5641 aaaatctgat ccattcttgg cgaacttagt agttctgatg gatttggtgg tttaggtcca
     5701 cttggcagaa tcgacaaatt gtcgactggt gttcgttgga tctcatctgc tacacttcct
     5761 gatgaactaa gaacagtact taatccgctt gcgttgttca acttgaacgt tttatagatg
     5821 acgggcttac gtaagtctgc atcgactagc agcacccgtt gaccagattt ggcaaaaaca
     5881 acggcaatgt ttgctgcagt ggtcgatttc ccttctccag gtcctgatga agtcacaaca
     5941 atcgttttga tttgctgacc agctgcggag gcaaactgga tattcgtccg aatcgtacga
     6001 tattgttcag cgattggtga agagggattg gtgagtgtga ccaaactgac tgcatgagtt
     6061 tgggctgctt tttgtttctg tgttcgtgcc attttctttc ctcctagact cttgagcggc
     6121 ttctgcgtga tggcgctgtt ttatcgtttg tagcttttgg ttgtgtctga gctggtcctt
     6181 ttttacggat accagcattc aattctttcg ctgacatttc tggaactgtt cctaagattg
     6241 ggaatcctaa tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca
     6301 ataagaatgc cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca
     6361 atttattatt tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt
     6421 tatcaacatt catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga
     6481 tctgttgagc agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt
     6541 ctgcctgatt aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt
     6601 ctgtttctaa acgatctttg acttcattga gcactaaatc actgacgatc atatctttat
     6661 acgtattgat catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg
     6721 gcagtgtcac gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta
     6781 cgattcctga gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc
     6841 gtaatgtttc aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct
     6901 atttattctc tagattcatt tgatctttta attctttttt cacacgatca agttcttgat
     6961 cactgacttt ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt
     7021 gcttaatcgt accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt
     7081 tcatgtctgt tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac
     7141 cgtttagact caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc
     7201 taccgtaatc accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc
     7261 cgttcaatgt aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat
     7321 tattgacttc gataccacca actgcatcaa cgagttcctt tagccctttc atattgatag
     7381 agacatagtg atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag
     7441 ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga
     7501 catacgtatc acgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg
     7561 caaccatcat cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc
     7621 ccatcaatag aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc
     7681 cttgggttcg ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata
     7741 attttgctcc tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca
     7801 gaaatatttt tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct
     7861 aaccttgctt gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct
     7921 cagcaacagt ttgtgctttt gttgcgtatg ctgcgcttat tttttcattc cggatctgct
     7981 ctatttgact gagcaccgtc aaatattgat cgtaagtagc ggtggcagtc acttgaccat
     8041 cctttaacat cgtatcgaat aactgatcta gttgcgtgac ttgcgcgatc tgaatatcag
     8101 cgaatcctag atattgcata acaaccgttt tccaaggact atctttaaaa aagctcatat
     8161 tttcacgtac acgagcgaca tctgcacttg tcaccttttc tttgatgatg acatcgtcta
     8221 cagcttgctg ccagtttgat acatttttag taaataaatc gttgacagct gtctggatct
     8281 tcagttttga ttctgcacta tctaactgtg tcacgattgc tttcttctca tcttggattg
     8341 caacagcttc tttaggcaat tctgattctt tgatgtcgta atcatctgct gaaagtttga
     8401 cagaggatag cttatttgac aaacttgtga ttgtttcttc tgttatttca gggtttagaa
     8461 aaattttttc ttttgtacta tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt
     8521 ctttttgatt ggtgatggca gattctgcat aattgatccg ttgctgatgt tgccgctgat
     8581 tattgatatg gtatccaaac aaacagatcg caatagcacc tgcacctacc gccacagcag
     8641 tcaatgactt gacgaatggc ttttccatag attcactcct aagtattgat gatagataaa
     8701 ttaagatata attcacttac ataaaaatga cagcttttta tttaaaaaaa cat
//
LOCUS       DACDCV010000105         8690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDCV010000105 DACDCV010000000
VERSION     DACDCV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.105"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..514
                     /locus_tag="IV041_002435"
     CDS             122..514
                     /locus_tag="IV041_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383182.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(687..869)
                     /locus_tag="IV041_002436"
                     /pseudo
     CDS             complement(687..869)
                     /locus_tag="IV041_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            856..1053
                     /locus_tag="IV041_002437"
     CDS             856..1053
                     /locus_tag="IV041_002437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383183.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1096..1671)
                     /locus_tag="IV041_002438"
     CDS             complement(1096..1671)
                     /locus_tag="IV041_002438"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8383184.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1826..2352)
                     /locus_tag="IV041_002439"
                     /pseudo
     CDS             complement(1826..2352)
                     /locus_tag="IV041_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2809..4320
                     /gene="gadC"
                     /locus_tag="IV041_002440"
     CDS             2809..4320
                     /gene="gadC"
                     /locus_tag="IV041_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8383185.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4333..5733
                     /locus_tag="IV041_002441"
     CDS             4333..5733
                     /locus_tag="IV041_002441"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8383186.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5898..7338)
                     /locus_tag="IV041_002442"
                     /pseudo
     CDS             complement(5898..7338)
                     /locus_tag="IV041_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7731..8324
                     /locus_tag="IV041_002443"
                     /pseudo
     CDS             7731..8324
                     /locus_tag="IV041_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8308..>8690)
                     /locus_tag="IV041_002444"
     CDS             complement(8308..>8690)
                     /locus_tag="IV041_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8383187.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2759 a         1447 c         1566 g         2918 t
ORIGIN      
        1 ttttttcttt taagtaaaaa atataggtga ttttttttga cttcatattt ataataaaga
       61 cacgtaaatc aattattttt cattttactt taactaataa tgtttatttg gagggattat
      121 tatgttagat gatagattga cgtgttctaa ttgtggtaca ttattttact atgaggaagt
      181 tagaaatatt gtaaaacata gaaaaaaaga aatgcctata cgttgtccaa attgcaatga
      241 agttattaag cgaaagttgt ctcatggtta ttttgtcagc tacgaactat atgaagaaag
      301 taaaataaat ctgaaaaaaa aaccaaaata ctataaaaat ttgaaaactg gagctattat
      361 aactgaaaaa gaatttaaaa atttagtgac tgaagaagaa agcaggtttt ataatttgtc
      421 tggtaatatt tgccctggat ttatggatca gattattgaa aatgagtcaa ttgaatcaat
      481 tgaaaactat gttcctgttg acttaccaaa atagaaaaaa taatcctgtt tttacagtga
      541 attattttaa ttttgatttt caataaaaat atattacaat aataatcgta atagctaatg
      601 aaacaatttc tcaatctttt taccactcca aacagggagc gtcaataatt ttgtgtaaat
      661 aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc
      721 acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg
      781 gatactagga aacgctctag tgattcttca ttttgaaact gctcttttct gcggctgtat
      841 ttcttgattt gtttattgaa agattcgatt gtagaaccag aaggaatgga gaacactttt
      901 gaactttaca acaaaactac tgttgataaa ttaattccaa ttttagttcc aactgattta
      961 aatggtcatg atgttttact tcgttttaac ggatttttag gtgaggatta tagataccta
     1021 aaaatttctg atcctcaacc tttaacagaa taataaaaaa cgtgtaatca attatttgat
     1081 tacacgtttt ttatttcaat cttcgttgta gtagtggttg cgacataccg aaaggtaatc
     1141 ttcattacct cctactacaa tctgttcacc tgaataaact ggaataccat caacaatacg
     1201 agcattcatt acagcctttt tattgcaaaa atggcaaata gttttaattt cctcaatttt
     1261 atcagcaaat gtcaataatg cttcggatcc cttgaagaga ttattttgaa aatcattctt
     1321 caatccatag caaattactg gtacttcctc attcattacg ataagtgata gatcaataat
     1381 attctctcta cttaaaaatt gagactcatc gaccaaaata caatcaactt ttccgtactg
     1441 atcaataatc tccactaatt ctccgtacac atccatttca gaagtaattg caatagctgg
     1501 tttcgaaaca cctatacgac tctcaacttt tccaaatcct gatcgagtat ctattgcgct
     1561 tgtcattatg ataactctct tcccttgttc ttcataatta tgagccactt tcagtagctc
     1621 aatactcttt ccactattca ttgttccata tctgtaaaat aattgtgcca ttaaatttat
     1681 tcccttcttt tacaattcaa ttattcctta attccatctg cttttatgca gctgctcgtt
     1741 tattccttga tatgtctata acatctacta tccctaaaac caatttataa gccattagag
     1801 atggtattcc tgaacatctc tcatattgtt ttttgagtgg tatatcatct gaaaaactga
     1861 aggaatcatg aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac
     1921 ttcttcagtc agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata
     1981 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
     2041 gacggtctcc tctaatgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa
     2101 ctcgccattg cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt
     2161 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc
     2221 gtaatcgagg aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg
     2281 gacacggaat aatccattca aatcgccttt gttcgctaga gtctttagga tttctgtagt
     2341 aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca
     2401 gaagggactt ccttttttct atttacacaa aatattttac actctcaaca aaagtatctt
     2461 cgattaatac ttgctcaagt tatgtgcagc tacgaaaaag taggtaccga ggtactttct
     2521 ttttcgcagc tttttttgga cttatgagaa gtacattcgc tggaagaata cgatataaaa
     2581 cgagcttttt cactaaagat attaatagag tgaaagaaac aagcttttgt tgtcagtttt
     2641 ttcacttttc taaaaatgaa aataaaaaat tctggaagtg gagaagaatt ttgcttgcta
     2701 tactttgtct tgtagaaaag aggtagacaa aataatgcaa gctcaaataa gcgattttcc
     2761 ataaccttgg taggaatgac aaattaaata ggaaagggaa gtaataatat gaataaaaaa
     2821 aagttatctc tattcggttt ttttgccctg acagcttcca tggttttgac tgtttatgag
     2881 tatccgacct ttgcaacatc acaattacat ttggttttct ttcttctttt gggaggtatt
     2941 ttatggtttc taccagttgc tttatgtgct gcagaaatgg caacagttga aggatggaaa
     3001 gatggtggga ttttcagttg ggttagtcaa actttaggag aacgttttgg ttttgcagct
     3061 atttttttcc aatggtttca aattacagta ggttttatta caatgatcta cttcatacta
     3121 ggagcgctgt cgtatgtaat taattttcca gagcttaata cagatccatt tatcaagttt
     3181 attggtgtat tagtcatttt ctgggtactg actttttcac agttgggcgg gaccaatcgt
     3241 accgctaaaa ttgctaaagc aggatttgtc attggaattg ttattccttc gatcattctc
     3301 tttggtttag cagcagccta ctttatcggc ggtaatccga ttcaaatggc tattaatgcg
     3361 aaagctttta ttcctgactt ttctcagctt tcaactttag tagtatttgt atcttttata
     3421 ttggcttata tgggcgtaga agcttcagca tcacatatta atgagctgga aaatccccaa
     3481 agaaactacc cattggcaat gtttttacta gtgattttgg ccatcgcctt agatgcaatc
     3541 ggcggtttct cagtagcagc ggttattcca caacatgact tgtctctcag tgctggtgta
     3601 atccaaacat ttgaaacgct tattttacat tttaacagtc atttacattg gttggtaaaa
     3661 gtgattgctc tcatgattgc ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt
     3721 ccatcacgag ggatgtttgc agctgctcag cgaggtctgt taccagtttt cttccggaaa
     3781 actaataaac aagacgttcc agttccgttg attatgatac aaggaattat cgttactatt
     3841 tgggcagcag tcttaacttt tggtggagga ggcaacaatt tatcattctt agtagcaatc
     3901 tccttaactg ttgtcattta tctcgtagga tatttactat ttttcctagg ttactttgta
     3961 ttagttttta agaaaaaaga cttaaaacga acttacaatg taccaggaaa aactgttggc
     4021 aaaacgatta tcggtggaat tggttttatt ttatcagtgt tcgcattgct tatctctttt
     4081 gtaccaccag cttcaattgc agctagtgag caccgaacat accaaatcat actacttatt
     4141 agctttgtag tgactgttct tttacctttt atcatttatg aagtacatga taaaagtgct
     4201 cacacaacta ttgaggaacc tactcacgtg aaagctgaag acgttaatcc cgctgtttat
     4261 ccagctgcta gaggagagca ccacgttaca aaaaatgatg aacatatttt aaaacattag
     4321 gaggaattca ttatgttata cggaaaagat aatcaagaag aaaaaaacta tttggaacca
     4381 atttttggct ctgcaagtga ggatgttgac ttgcctaaat ataagttaaa caaagaatcc
     4441 attgaaccac gaattgctta tcaattagta caagacgaga tgttggatga aggaaatgcg
     4501 cgattaaact tagctacttt ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg
     4561 acccaaacgt tagaaaaaaa tgcaattgat aaatcagaat acccacgaac aacggaaatt
     4621 gaaaaccgct gtgtaaatat gattgctgat ttatggcatg ctccaaataa tgaaaaattc
     4681 atgggaactt caacgatcgg ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa
     4741 tttgcttggc gtaaacgtgc tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac
     4801 ctggtgatct cttctggtta ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg
     4861 gaactgagag aagtcccaat ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg
     4921 gattatgtgg atgagtacac aattggtatt gtaggtatta tgggtattac ttacactggt
     4981 cgttatgatg atatcaaggg tctgaatgat ttagttgaag ctcacaataa acaaactgac
     5041 tataaagtat acattcatgt tgacgctgca tcgggtggct tttatgcacc atttactgaa
     5101 cctgatctag tttgggattt tcaattgaaa aatgttatct caattaattc ttcaggtcac
     5161 aaatatggtt tggtatatcc aggtgtgggt tgggtcttat ggcgtgacca acaatactta
     5221 ccagaagaat tagtatttaa agttagttac ttaggtggag aaatgccaac tatggctatc
     5281 aacttctctc atagtgcagc acaactaatt gggcaatact acaactttgt acgctatggc
     5341 tttgatggtt atcgtgatat tcaccaaaga actcatgatg ttgctgttta tttagccaaa
     5401 gagatcgaaa aaactggtat ttttgaaatc attaatgatg gatcagaatt gccagttgtg
     5461 tgctataagc tgaaagaaga tcccaatcgc gaatggacac tatatgattt atctgatcgt
     5521 ctgttaatga agggatggca agtcccagcc tacccattac ctaaagactt ggatcaatta
     5581 attattcaac gcttagttgt tcgagcagac tttggaatga acatggctgg tgattatgta
     5641 caagatatga accaagcaat tgaagagttg aataaagctc atattgttta tcataaaaaa
     5701 caggatgtga aaacgtacgg ctttactcac taaaattctt cagagaaata taaaagaagt
     5761 taattaacta ataaaaggag ttgtgattac cacacagctc cttaaatcta tttgaataaa
     5821 tatatgatat tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct
     5881 tattgtaagt agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt
     5941 tgaaaagtaa tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa
     6001 ctatcgcaaa gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt
     6061 tgtttacaaa tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt
     6121 cgatagtata aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc
     6181 cgtttatgct catttaaaag acttaaacta atggttagtt tacgaataga attctcattc
     6241 caacgcagaa ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag
     6301 tacaaagcgc gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata
     6361 tcagcccaaa gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct
     6421 tcagatatat tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct
     6481 gctacatggt tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat
     6541 agataatttc tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat
     6601 aaagagaaag aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg
     6661 gcaactcttt tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg
     6721 atagcgatac ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaaa
     6781 tgtttttcta cataatcaat caattgttga ccgctttgaa taattgattt atttagctgg
     6841 atcaactctg tccgatttgt ctcatagatt atcgttagta acaaacttcg ataatccttt
     6901 tcaggaattt caaaatggcc atcagcattt aagtactgca tttgtttaaa aaaattaatc
     6961 aactcatttc gtaagttgta aactttgctt aacgaaatga aggtagtatc agctatatta
     7021 ctccaattgt gttcccctga taaataatgt cctagtacat tgaaaaagtc agagcgctga
     7081 tagatgagtt gagtgagtgc caacctcgaa aacgaagagt cgaatgcgac tgtaaagcta
     7141 cttctagatt tctccactga aataatttgc tgatggagta tgtcggtaat gcattccaag
     7201 tcgttagtga cagacagtga gtgggcaaac gttcaattga tctgctattt cgcgaatctg
     7261 aatggtccct ttttcgtgta attgctcaca aatgtaaact tgacgaaaaa tacttttctc
     7321 cagataggtt tccaacatag tactcctcct agtcatttta tacataacta ttataactct
     7381 accaatcgga atagtcctgt aatatattga atcaaaaata taaattataa aataattaga
     7441 tggggtattt ggtttaagta tcccttatat agtaataaat ttataggtgt gattctcgaa
     7501 ttaatacccg aaaatgtgag agaattttta tggatttttt aggatacaaa ttaattattt
     7561 ttgcttttat cctgattacc agtccagtac tgtcggtatc ccattttgca gcaaaatcat
     7621 gttgataaag tgacaagaag actgatgaga tatattatcc aaattgagaa gataattaat
     7681 cggattcttg agtataccct actttttaag aaagtagggt atacaagcgt atgaataaag
     7741 acataacttt ccctgtgatg gacgttcggc ttgttccaat taccaaaata caggcgaacg
     7801 attataatcc caatcgagta gcgtctccgg aaatgaactt attggagctt tctatcattg
     7861 aagatggctt tactcaacca ttagtttgtt attacaagga aaaagaagag aagtatatct
     7921 tggttgatgt gcttcatagg tatcgtgttg ctaaggaaag actcaactta aaagttgtac
     7981 cggtaacagt tattgataaa cccatataag aacgtatggc ttcaacaatt cgacacaatc
     8041 gtgcaagagg gacccatgaa ataagaggaa tggaaattgt catccaaaga ttagtaaaaa
     8101 atggatggtc agacaagaga atatccaaag aattaggcat ggacattgaa gaaatttttc
     8161 gctttaagca agtgtctggt ggaatggcaa cagttttttt gacaaatttt ataaggtgca
     8221 gaacttcttt ccgtatgcta ttccgattgt ccttgacaat gagcctcctc ggatgtgacg
     8281 atcttcggca ggagctaaca gttaaagtta gactgcctcg ctagcgttag cacatccgag
     8341 ctcattatca aggattcgct gcgccaatct ctggttacgg taaccaaccg gtgtctcgta
     8401 gccaagtgta ccgtgcaacc gaaggtggtt ccaccaattt acatagtcaa ataactccaa
     8461 atccaattgt tgtaaggttt caaatgtgta ttgatagaca aattctactt tcaacgactt
     8521 ataagttgat tcagctacgg cattatcaaa aggacagcct ttatgactca atgatcgatt
     8581 gatgtcaaaa gttgttaata attcatcaat agcttggtta tcaaactctt ttccacgatc
     8641 agtatgaaaa atctcaacct ctgtcagagg ttgtttgata cggctaaatg
//
LOCUS       DACDCV010000106         8511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDCV010000106 DACDCV010000000
VERSION     DACDCV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.106"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IV041_002445"
     CDS             94..1248
                     /locus_tag="IV041_002445"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8383188.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IV041_002446"
     CDS             complement(1270..2022)
                     /locus_tag="IV041_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8383189.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IV041_002447"
     CDS             complement(2386..5052)
                     /locus_tag="IV041_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8383190.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IV041_002448"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IV041_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8383191.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV041_002449"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV041_002449"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8383192.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8282..>8511
                     /locus_tag="IV041_002450"
     CDS             8282..>8511
                     /locus_tag="IV041_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383193.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIE"
BASE COUNT         2523 a         1746 c         1504 g         2738 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8461 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa a
//
LOCUS       DACDCV010000107         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDCV010000107 DACDCV010000000
VERSION     DACDCV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.107"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV041_002451"
     CDS             complement(<1..173)
                     /locus_tag="IV041_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8383194.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV041_002452"
     CDS             complement(593..1714)
                     /locus_tag="IV041_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8383195.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IV041_002453"
     CDS             2042..3451
                     /locus_tag="IV041_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8383196.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV041_002454"
     CDS             3448..4176
                     /locus_tag="IV041_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8383197.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV041_002455"
     CDS             complement(4304..5215)
                     /locus_tag="IV041_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383198.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV041_002456"
     CDS             complement(5232..6239)
                     /locus_tag="IV041_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8383199.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV041_002457"
     CDS             complement(6236..8365)
                     /locus_tag="IV041_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8383200.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV041_002458"
     CDS             complement(8370..8477)
                     /locus_tag="IV041_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383201.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDCV010000108         7974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDCV010000108 DACDCV010000000
VERSION     DACDCV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.108"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1385)
                     /locus_tag="IV041_002459"
     CDS             complement(45..1385)
                     /locus_tag="IV041_002459"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8383202.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(1622..3145)
                     /locus_tag="IV041_002460"
     CDS             complement(1622..3145)
                     /locus_tag="IV041_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8383203.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(3523..4134)
                     /locus_tag="IV041_002461"
     CDS             complement(3523..4134)
                     /locus_tag="IV041_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8383204.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(4257..4382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4464..5180)
                     /locus_tag="IV041_002462"
     CDS             complement(4464..5180)
                     /locus_tag="IV041_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8383205.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(5186..5755)
                     /gene="scpB"
                     /locus_tag="IV041_002463"
     CDS             complement(5186..5755)
                     /gene="scpB"
                     /locus_tag="IV041_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8383206.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(5739..6536)
                     /locus_tag="IV041_002464"
     CDS             complement(5739..6536)
                     /locus_tag="IV041_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8383207.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(6506..6877)
                     /locus_tag="IV041_002465"
     CDS             complement(6506..6877)
                     /locus_tag="IV041_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8383208.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(6896..7783)
                     /gene="xerD"
                     /locus_tag="IV041_002466"
     CDS             complement(6896..7783)
                     /gene="xerD"
                     /locus_tag="IV041_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8383209.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         2272 a         1730 c         1347 g         2625 t
ORIGIN      
        1 acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa
       61 cgatacacag cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt
      121 tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac
      181 tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca
      241 gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat
      301 aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt
      361 aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt
      421 tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt
      481 tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct
      541 aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata
      601 gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct
      661 tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc
      721 ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca
      781 gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa
      841 attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta
      901 tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt
      961 gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa
     1021 ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg
     1081 aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag
     1141 ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct
     1201 ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc
     1261 acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt
     1321 cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt
     1381 ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac
     1441 ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa
     1501 caaatgatga aagaaaaaac actcactgaa tggagctatt gacttgtcca cgtgcttatc
     1561 gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg
     1621 tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta
     1681 atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca
     1741 ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag
     1801 tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg
     1861 ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa
     1921 atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta
     1981 agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta
     2041 ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag
     2101 actctttcat gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa
     2161 tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta
     2221 ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg
     2281 ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa
     2341 catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct
     2401 tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg
     2461 atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc
     2521 gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa
     2581 aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag
     2641 ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa
     2701 tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg
     2761 gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta
     2821 gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga
     2881 ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat
     2941 tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg
     3001 atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag
     3061 taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa
     3121 attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa
     3181 ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt
     3241 ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcagtttt
     3301 ttaaagcttt cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt
     3361 cttttaaaag gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc
     3421 aaaaaaatac tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt
     3481 tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg
     3541 acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac
     3601 taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag
     3661 tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa
     3721 gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt
     3781 tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa
     3841 ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa
     3901 attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc
     3961 aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc
     4021 gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc
     4081 gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa
     4141 tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac
     4201 tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct
     4261 atcttcttta tccagactat actgtcggta tcggaatttc accgattcag ctattttttt
     4321 gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga
     4381 accttatata atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc
     4441 taacaattat aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt
     4501 ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt
     4561 tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga
     4621 ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat
     4681 aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa
     4741 ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact
     4801 tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc
     4861 cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa
     4921 agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt
     4981 ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca
     5041 gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca
     5101 cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt
     5161 actttctgca atctctccat tacaattatt ctcctttatt cattccatca aagaatagat
     5221 ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta
     5281 atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac
     5341 ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg
     5401 ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat
     5461 aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg
     5521 catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg
     5581 tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca
     5641 ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga
     5701 tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg
     5761 gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg
     5821 atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta
     5881 gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct
     5941 tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact
     6001 ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt
     6061 ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt
     6121 tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat
     6181 ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat
     6241 aattcttcat cgtctgtgcc gaattcttgg acaggaagca atgtcttgct tttgattgcc
     6301 atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt
     6361 gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg
     6421 atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat
     6481 ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc
     6541 caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta
     6601 attcatctaa catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt
     6661 catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg
     6721 ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa
     6781 tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag
     6841 caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag
     6901 cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa
     6961 tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc
     7021 cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt
     7081 aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac
     7141 ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac
     7201 gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc
     7261 gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca
     7321 atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg
     7381 cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt
     7441 ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca
     7501 tcggatcatg atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg
     7561 agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac
     7621 ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat
     7681 gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt
     7741 caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc
     7801 ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag
     7861 aaaaagccgt ctagacactc agacggactg gtttcttttt tcggctctat aatttatggg
     7921 actgatgaat cagaattgat tcttcagccc cattttcatt ttaggtatta aaaa
//
LOCUS       DACDCV010000109         7697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDCV010000109 DACDCV010000000
VERSION     DACDCV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.109"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IV041_002467"
     CDS             complement(<1..179)
                     /locus_tag="IV041_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8383210.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IV041_002468"
     CDS             complement(192..1295)
                     /locus_tag="IV041_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8383211.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IV041_002469"
     CDS             complement(1385..2320)
                     /locus_tag="IV041_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8383212.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IV041_002470"
     CDS             2622..4556
                     /locus_tag="IV041_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8383213.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IV041_002471"
     CDS             5013..5429
                     /locus_tag="IV041_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8383214.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IV041_002472"
     CDS             5443..6009
                     /locus_tag="IV041_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8383215.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IV041_002473"
     CDS             6032..7018
                     /locus_tag="IV041_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8383216.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="IV041_002474"
     CDS             complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="IV041_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8383217.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2430 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgt
//
LOCUS       DACDCV010000110         7637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDCV010000110 DACDCV010000000
VERSION     DACDCV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.110"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IV041_002475"
     CDS             <1..177
                     /locus_tag="IV041_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383218.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IV041_002476"
     CDS             190..693
                     /locus_tag="IV041_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8383219.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IV041_002477"
     CDS             753..1142
                     /locus_tag="IV041_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383220.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..3576
                     /locus_tag="IV041_002478"
     CDS             1129..3576
                     /locus_tag="IV041_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8383221.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3581..5704
                     /locus_tag="IV041_002479"
     CDS             3581..5704
                     /locus_tag="IV041_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8383222.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5701..6705
                     /locus_tag="IV041_002480"
     CDS             5701..6705
                     /locus_tag="IV041_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8383223.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6724..7635
                     /locus_tag="IV041_002481"
     CDS             6724..7635
                     /locus_tag="IV041_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383224.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGIWKIVDVK"
BASE COUNT         2425 a         1268 c         1732 g         2212 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg ggaacggggt
     2641 ggctggaaag aaaccttgcc agaaattgcg gatgaaatca atattctcaa cttaactagt
     2701 aagccaaaaa atcaaggatt acttgatcct tatgtgatta tggagaaaaa gaaggattct
     2761 gaaagtctag caattgatat tctgaccttt ttaacaggaa tttctagtcg agatggggag
     2821 aagtttccag tgttgcgtaa ggccattcgg acggttaccc aaagcaatca acggggatta
     2881 ttacaagtaa ttaatgagct tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat
     2941 cacatcgagt cctttaccga ttatgatttt gcccatttgc ttttctcaga tggttcgatt
     3001 acccagtcga ttagtttaga taggcagtta aatatcattc aagtagcgga tttggtttta
     3061 ccagatgcag aaaccacttt tgaagaatat accaccatgg aattgcttag tgtttccatg
     3121 ctaatcgtca tttccacttt tgccttggac tttatccata gtaatcgaga aatcttcaaa
     3181 attgtagact tagatgaggc gtggagtttt cttcaagtag cgcaagggaa aacattatcg
     3241 aataagctcg ttcgtgctgg gcgggctatg aatgccgggg tttactttgt cacgcaaaat
     3301 agtgatgatt tactggatga acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc
     3361 agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggcttgga tcgagaagat
     3421 gaaagcaatc aaaaacgcct acgagatttg gaaaatggcc aatgcttgat gcaggatttg
     3481 tatggtcggg taggcgtggt tcaggtacat ccagtctttg aagaactctt ccatgccttt
     3541 gatacccgtc cacctgttca agagagaatg gagtgagggg atgaaaaaga aactttttcg
     3601 gattgtggga atcgttttta ttagcatact cactctgata gttctattga gtttagttgg
     3661 tacggtagcc gaggcgacag ggctagttga tgatacagtt gaagcaggaa atctatattc
     3721 caaatattca ctgaacaact atcaactaga tttctttgtg gatagctctt gggattggtt
     3781 gccttggaat tggggtgatg gcttagggaa aagcgtgatg tatggtcttt atgccattac
     3841 taatttcatt tggacggtga gtttgtattt gtcgaacgcc acgggttatg tggttcaaga
     3901 ggcttacaaa ttagatttca tttcggatac ggctgaaagt attggaaaga atatccaaac
     3961 attggctggt attactgaaa atggactcca gacctccgga ttttattttg ggtttctatt
     4021 attgatgatt ttagctctgg gggtgtatgt ggcgtatacc ggattattaa aacgagaaac
     4081 cacgaaagca gttcgagccg tgattaattt tgtgatgatt tttctactat cgggttcgtt
     4141 cattgcttat gcgcctacct atatcacaaa aatcaatgac tttagctcag atgttagtga
     4201 agcggccctt acccttggaa ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga
     4261 tagtgtggat ttgattcggg atagtttgtt ttcgattcaa gtccaacaac cttggttact
     4321 actgcaattt gatgattcca acatagaaga aattggtgaa gaccgggtca acaagattct
     4381 atctgtgagt ccagatgaaa acaaaggcaa agatcgggaa gaagcggtca aggcagagat
     4441 tgaagacaat gacaatgcga atctcagtat taccaagacc atgaaccgtt tagggaccgt
     4501 ggtctttttg gtattgttca atattggcat ttccttcttt gtttttcttc taacagggat
     4561 tatgttgttc tcgcaaattc tcttcattat ctttgcgatg tttctgccaa ttagtttctt
     4621 attgtccatg ttgccgactt atgaaagttt aggaaagaag gcgattattc gtttattcaa
     4681 tacgattatg atgcgagcgg gtgttacttt ggtgattacg actgcattta gcatctccac
     4741 gatgtttttc aatatttcgg caacctatcc atttttcatg gttgcttttc tgcaaattgt
     4801 gacctttgcc ggtatctatt tcaaattggg cgacattatg agtatgttca acctccagag
     4861 taatgatagc cagtcaatgg gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg
     4921 aaaattgcgg caactcaatc gaaatgtggg acgagctttg gcttttggtg gtggtgcagc
     4981 agtcggaaat aagttggcaa aggaacaatc aaaatccaaa tttaagccat ccggttcttc
     5041 tctacgaaag aattcacgat taccgaacga tcacgaaata ctatctgact taactaagga
     5101 gaatccagtt tcaaataata agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg
     5161 caaagttttg gatacccaag ctttggtgaa agataagact aaacaagtaa aggatcaggt
     5221 aaggaatacg ccaacgaatt tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa
     5281 agcaccagaa gaattcaagc gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg
     5341 agaaaaacaa cgacaacgaa gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga
     5401 agcaacaaat cgtcagggaa aaagaagggc gaatattcca atagataaag cagctcaacc
     5461 tcagaaagat atgatgctga aaaaagaagc accaaagcgg attgtgagag caaatctgaa
     5521 tcccgaaatc aaacgaaaac taactagtca agaaatcatt tcaaaaggga aaagccaatc
     5581 agcaagtaag aaaaattctt tccaacaagt gaaacagagg tccactctgc caaagcagac
     5641 caatcaaaaa gtacaaaaaa gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa
     5701 atgaacttga agaaagtggg tgttatttca ttaacttttc tgaccatctc ttttatgggt
     5761 attttgatgt gtgtgggttt gttgtttggt gaagacagtg agggaaatgg tagttctggc
     5821 acttctcctg gcggaactag tgtctcagaa gaagtattga aacaccgtgc aatggtggaa
     5881 aagtatgcca aagaatcgga tataacagag tatattccaa ttcttttggc gattattcaa
     5941 gtggaatctg gtggcacaat ggaagacgtt atgcaaagtt ctgagtcagc cggattacca
     6001 cctaattcat taagtaccga ggcatccatc aaacaaggct gtctctattt tgcgtctttg
     6061 gttaagcgtt ccaaagaact agggtgtgat caagatagta ttattcaggc ctataactat
     6121 ggtggcgggt atctagatca tgtcgggaaa aacgggaaga agcatagttt tgctttagct
     6181 gaatcttttt caaaggaaaa atcaggtggt caaaaagtcg attaccccaa cccaatcgcg
     6241 attaaggaaa atggtgggtg gcggtataat tatggcaata tgttttactg tttattagtg
     6301 aagcaatacc taactacaac tcagtttgat gacaaaatag tccaagaaat ttttgatgag
     6361 gcatttaagt atgaaggaac ggcctatgtg tttggtggct caagtccaga aacggggttt
     6421 gattgtagtg gcttgaccca atggtgttat gccaaagttg acctgaaact tcctcgggtg
     6481 gctcaggatc aatatgatgc catgactcat attgatctaa aagatgccaa accaggtgat
     6541 ttagttttct ttcattcaac ctatgatgcc ggtacttatg tgacccatgt gggcatttac
     6601 gcaggggaga atcgcatgtt tcatgcagga gatccagtgg ggtggacgaa tctaacggaa
     6661 aactattggc ggcagcatct tatcggagct ggccgataca aataagagaa aagaggaaaa
     6721 agaatgaaga taaaaattga acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa
     6781 cgagttgtat ccgtgggtaa gcatcgaaaa atggtgcttg ccctttggct tttattgtct
     6841 tgtagtcttg cttttggcgt ttataaaaat ttcaccgcca ttgatcaaca tacaactcat
     6901 gaaaaagtgg ttataaaaga gaaggtagtg aatacttctg gcgttgaaag ttttacagag
     6961 gattttgcga aagaatattt ttcatggaaa aataacaaag aagcaattga aaagagaatg
     7021 agtaatctcg aacagtacct tacagaaaac ggtctcgctt taatccaaga tatggttcga
     7081 gcagatattc ccacgagttc cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac
     7141 tcggaagaat ttgtcgtttc atttttagtg gagcaacata ttacagaggg aaaaaagacg
     7201 caatcaattt cttctgcata tcgagtgaca atctttgaag atgaaaacag gaatcattta
     7261 gtaactagtt tgccaacaat gattggaaag ccagacaagg caaaatatga agcaaaacaa
     7321 gtagaaaacg attcagaaat tgatgccaaa acaacggaag aaatcaccga ttttttagaa
     7381 acatttttta aactttatcc atcagcttct gaaaaagaat tggagtatta tgttgaaaat
     7441 ggtgcgatgc gaccaatcaa tattgactta aagttttcgg agttagagaa tccagtttat
     7501 ctacaaaacg gaaaaaattt tctagtgagt acaaagataa aataccttga taatgtatca
     7561 aaaaccacta gctactttca gtacaaatta gtattacaaa aaaatggaat ctggaaaatc
     7621 gttgatgtta aataata
//
LOCUS       DACDCV010000111         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDCV010000111 DACDCV010000000
VERSION     DACDCV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.111"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IV041_002482"
     CDS             complement(138..428)
                     /locus_tag="IV041_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383225.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IV041_002483"
     CDS             complement(546..806)
                     /locus_tag="IV041_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383226.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IV041_002484"
     CDS             complement(943..1356)
                     /locus_tag="IV041_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8383227.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IV041_002485"
     CDS             complement(1812..2297)
                     /locus_tag="IV041_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383228.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IV041_002486"
     CDS             complement(2436..2651)
                     /locus_tag="IV041_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8383229.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IV041_002487"
     CDS             2875..3675
                     /locus_tag="IV041_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8383230.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IV041_002488"
     CDS             3694..5562
                     /locus_tag="IV041_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8383231.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IV041_002489"
     CDS             5555..6046
                     /locus_tag="IV041_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8383232.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IV041_002490"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IV041_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8383233.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IV041_002491"
     CDS             6606..>7600
                     /locus_tag="IV041_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8383234.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDCV010000112         7517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDCV010000112 DACDCV010000000
VERSION     DACDCV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.112"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(198..875)
                     /gene="nth"
                     /locus_tag="IV041_002492"
     CDS             complement(198..875)
                     /gene="nth"
                     /locus_tag="IV041_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8383235.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(882..1580)
                     /locus_tag="IV041_002493"
     CDS             complement(882..1580)
                     /locus_tag="IV041_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8383236.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1848..3032
                     /locus_tag="IV041_002494"
     CDS             1848..3032
                     /locus_tag="IV041_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8383237.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3038..3481)
                     /locus_tag="IV041_002495"
     CDS             complement(3038..3481)
                     /locus_tag="IV041_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8383238.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3707..3994)
                     /locus_tag="IV041_002496"
     CDS             complement(3707..3994)
                     /locus_tag="IV041_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383239.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4165..5517)
                     /locus_tag="IV041_002497"
     CDS             complement(4165..5517)
                     /locus_tag="IV041_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8383240.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5790..6236)
                     /locus_tag="IV041_002498"
     CDS             complement(5790..6236)
                     /locus_tag="IV041_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8383241.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6472..7398)
                     /gene="cysK"
                     /locus_tag="IV041_002499"
     CDS             complement(6472..7398)
                     /gene="cysK"
                     /locus_tag="IV041_002499"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8383242.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2028 a         1543 c         1234 g         2712 t
ORIGIN      
        1 ggcattattt tctacatcgg gtattttccc aataaaaata acaactttta tttttatcgc
       61 aataaattgt aataagttac catcctagct actgagtatg tacatttact ttttcatgca
      121 aaaaagataa acgaaacagc ttttctccat aataacaaga taattcatac atttatttgc
      181 aattaaagaa accacgtcta caggcgtggt ttctttttta gcgccttttc ttttagacgc
      241 atccgatttt tcccatcttg acatattgat agcaaaggac atacctcgca cttgggattt
      301 cttgccgtgc aatgatatct accaaaaaaa atcagtgtat gatgtgtctt tacccataat
      361 tcttgtggta ctttccgcat cagtgtttct tctacttcca tgaccgtagc atctagtttg
      421 cagatcctca ggcgttttga gacacgttcc acgtgggtat ctactgcgat tgctggtatc
      481 ccgaatgcat cccccaatac gacatttgct gtctttctgc ctacaccagg aagagacatc
      541 aattcttctc ttgacgttgg cacttgccca tcgaaacgtt caatcagctg ctgtgcacac
      601 gctttgatgt tttttgcttt atttcgatac aaaccaatcg tttttatttt aagaatgatt
      661 tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc
      721 gttgctttat tcactgatac atctgtcgcc tgggcactta agatcactgc aatcaataac
      781 tcaaatggat tattgtgctt gagttctcca tgtgcttcgg gaaacattcc atacatcgtt
      841 tctagagctt ccattgtttt ttgtttgctt aacatattcc gctactcctc caaccaattt
      901 ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact gttctacttc tctttgaaga
      961 agctgctgtt ttcttcgttt ttggtcttct tccacttgtt gttttgtacg tagatttttt
     1021 ctttcccacg atagcaaaat gcggtcaacg taattaaaac tataagcctg atttaatacg
     1081 gcttcccgca gtgctagctt caaaatttcc ggctgataat ggtcttcttc tagccattgt
     1141 ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa attcttgttc aaataattga
     1201 tacactgact ggatttcttt ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt
     1261 aaatactcac ctaatagatc atagatgggg taaagattat atcgatctgc ttttttaccg
     1321 ttgacaatcg tcgtttctat cttgataaat ccatttgtta cgagacgatc cagtatttga
     1381 aaaatctgat cttgtttgat tcccatatcc atagcaatca tctgcaaatc agggaaagta
     1441 tctccttcaa gctgtgccat atgcaactgt agaacaaata aaaattcttc agattgtaga
     1501 ccgatcctat gataattttt aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag
     1561 taattttcta aattcaacat gatttctcct ccgctcaatc acagtatacg taaaaaaaac
     1621 atgggaagca agaacaggtt tcttttctat ttacttcact gatcggtcag atttacaatg
     1681 tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt atcttcactt ttcggaataa
     1741 ataaattaag tagatttcgc tgttccttag aatgttgaca aggaaacaaa attactatac
     1801 actatttttg tttccaagtt aacattattg cataaaggag tttttacatg tacagtttca
     1861 caaataaaca ttctaaagtt tttggatacg tctctgtctt attcactgga ttaggattta
     1921 ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta ttctcactcc atacatcagc
     1981 aagctttcta tatcactttg ctcatgtctg tttatgctct agctgccttc ttatctgccc
     2041 ccattctcgg tagcttaagt gatcattttg ggcgtcatcc gatccttatt atcagtttat
     2101 taggatcaag tatcggatat ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc
     2161 ttggtcgtat cattgaagga ttgacggcag gagaaatctc caccttatat gcttactttg
     2221 ccgacatcac ggaacctaat gaaagaacca aagtttttgg atggatgggt gctttagtcg
     2281 gtataggaac aacgctgggt cctattatag ggggtctatt agctgagttg gggaattctg
     2341 ttcctatatt tattggagca ctctttactt tcttaaatgc cgtttatggc tacacattta
     2401 tgctagaaag ccttccaata aaaaaacgtt cagtagattt aagtttctcc catgtaagac
     2461 cttttcacca attacaacaa ttattcaaga tttcatccgt tattccttta ttgacagcag
     2521 gttttgctgt ttggttggca gctggctctc ttcaatctat cttttctcaa ttttcgattg
     2581 acgcctttca gtggaaggct gggttggttg gtttaagctt ctctttgatt ggaatcttgg
     2641 atactgtctc gcaattgttc attatgcctc gattgctcaa aaaattttca gaacagcaga
     2701 tcacaagaat agggatgttc agtgaaatcc tcgcctatct attcatcaca ttatctggaa
     2761 ttcttctact gccgattttg tttttatttg ggattatctg ttatggtttc ggtgattcta
     2821 tttttactcc tgtattcaat ggccaactat ccaattctgt ctcagataac cagcaaggtc
     2881 ttgtaatggg cggtacccaa agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg
     2941 caggacagct ttatgctgca gcaccatttc ttccgacggt attcggattt ttgcttatga
     3001 tttttgccta ttatcactac aataaaaaat agtagcttca tttgctactt cgtataaatt
     3061 ctctgtatcg accgataaaa cgaaatactg tgtcaaattc ttcttttgac atattttcaa
     3121 atacagtagc atctcgctgt acccattttt gatggagttg ctcgtgtcta ttccttattt
     3181 tttgtccgat tggcgtcaat tgaaaataga tttctttttt attctcttcg ctttgataag
     3241 tgtttatgat ccctctagct atcagttttt ttgttagctt actgatggct cctctagtca
     3301 tgtaactagc attcgctaat tttgtcacgt taggatcagg gattttcccg atatgttcaa
     3361 tcacttctag ttcatttaga gtaaatcctg caaattcttt ttccatttct ggtttattac
     3421 gatgaataat ctcattagct aaatccagat attcttctat tagcagttgt cttttatcca
     3481 tttctacacc ttctttttct ttcattatat tgtttttttg tttcctattc aacaaagata
     3541 aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa aaaggggttg acaaagcttg
     3601 tcataacccc tttttctgat taaaagatag taaagagaca gtcatcggta attgtgatga
     3661 ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat
     3721 cagtttctag cacatatggc tgaccttcat atgtgtactc tgtattcaat gtgccgtcat
     3781 tttcatctga gtagatgcgt ctactgaaag gaaaacgatg agaaacatta tctttggcgt
     3841 catttgctgg catcgtgtaa tattcctctt gcgtttcatt caactgagag agtacttgat
     3901 ctgccaactc atcaatattg atcgttaatt tcttcgaagc acttttataa tcttctaaaa
     3961 atgcatcttg ttggacaaaa tattctttat tcatcttctt tctcaccttt cttttcaaaa
     4021 tcaaataaag ctgcttgttc ttttatgtaa cagctttatc tttgctgctt attgtgacaa
     4081 aaaattcgaa gaaagacaac taattgtact tttcacaaaa aaagacagag ggtcgtgaca
     4141 aaattgtgtc acaacccttt ttctttattg tgcagtttcg atttcgccta gcgctgcttg
     4201 tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt ggttggaaga agaaatctga
     4261 aagtgcgaga tcttctacag tcattttatt ttgaacagct aaagacatgg tatttgctga
     4321 ttgtgtaatg tcgtatttgg acatgaactg agcacctacg atacgattcg ttcctttttc
     4381 atagactagc tccatcatta ctttttcagt agttggcatg aactctggac gataattatc
     4441 ttcaaataca gtcgcttgga cttctagatc gttcaaagtt gcgctttctt tggtcacacc
     4501 tgtcgaaccg atcgtccagc caaataggta aagtcctgaa gttccttgag taccacgata
     4561 cgccatttta tgatctgtca agttatttcc cacaagcaaa ccttgacgaa cggcatttgt
     4621 tgccaaagga atatagtttt gcgtgccact tggattgtag ttgactactg cagagtctcc
     4681 agctgcaaaa atgtctggat tacttgtctg catgtattca ttcactttga ttgccccgtt
     4741 tggcagtgta tctacttttc cttcgatcag ttttgtattt ggacggaagc ccacacataa
     4801 aatgaccata tctgtatcaa atgtatctgt tggtgtagct acttgtttca cttttccttc
     4861 ttcatctgtg ataaattcag taacattttc gcctagcgca agagtaacac cgcggtctgt
     4921 caattctttt tctaagatat ctgtgaatgg tttatctaga tatttattca agatgcgatc
     4981 taatccatca atcaatgtca cttgttttcc agattcgaca aaagcttcta ctaattcaat
     5041 accgatatat ccaccaccaa caattactac acgttttgct tctttcgctt gtgcaataat
     5101 ttcatttgct tgattgtagt ttttgcatag gagtacattt ttcgcatcga ttccactaat
     5161 cggtgggata ataggccatg aaccagttgt catcactaat ttatcataag aaaccgtttc
     5221 tgtttctcct gttttcagat cttttgccgt tactgtttta ttttctgtat cgacgtctgt
     5281 tacatcgtgt tccattttaa ctgttgctcc taatgaagat aattcttcag gatttgagta
     5341 gaacaagcct gctgggtcct ttactactcc gccgacataa agtgcgatac cgcaagataa
     5401 gaaagaaata ttgtcatttc tttcatatac tgtcacttct gcttctggat gattttttaa
     5461 aatacttttt acagctgcag tacctgcatg agtacagcca attactacta ctttcattta
     5521 tggtccctcc gattaacatt ttgtccttcg acaaaactac tataacagaa ataaaacgga
     5581 tacaacagtt taatgcttgt gaattcacaa aggtttgttt gtagtttcat tccttttcaa
     5641 gcataagaac ccaatatcta caagattctt attttaataa tattcacttt tatatctaag
     5701 attgtgaata aaagacaaaa aaataaaaaa gctagcgttt tttttacaca gctagctttt
     5761 tttaggtata acacttgtag aaactgtttt tacatttgcg ccttttttgc tgactcttta
     5821 caatccggac agagtccata caattccaac cgatgttcat taatctcaaa atcagtcagt
     5881 ctgcttgcag ccatttctat atcttcaagc cctgggtaat gaaaatcaac aatcttaccg
     5941 cagttttcac aaattacatg ataatgacgc tttgaactga aatcaaatcg gctagaagca
     6001 tcgccgtact tcatttcttg aacaaatcca atcgaagtaa ataagcgaag gttattgtaa
     6061 acagtcgcaa cactcatatt tggaaaccga tgttcaagtg ctttataaat ctcatcagcc
     6121 gttggatggg aatgatgttc aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt
     6181 cgaatgttct ctttttttaa cttttcgata acctcttcga ctaacttatt ttccatgtaa
     6241 acaccccacg ttgttattct cgtcttatca tactattttt acattttaaa atccacttaa
     6301 catgaaaaga aaattcaatt ttattgtatt ttaatgaaaa aagacgtaaa acagtcgcgt
     6361 tcactattct acgtctctat tcaaataatt tttctcatca taaatttttg tgtgttcttt
     6421 tcatctttaa aagtttttta cataaaccta tctggagaaa aaccaagtgc cttatatctc
     6481 ttggtaaagg gctgtagata aataacgttc accattgtcg ggaaccacag ctaatacctt
     6541 cttgcctttt cctaattctt ttgcgacttt tagcgcggca gcaattgcag cgccagatga
     6601 gatgcctact aaaatccctt cttttcgccc aacttctcta gctgtttcca tcgcttcgtc
     6661 tccactaata gaaaggactt tatcatacac agaagtatcc agtgttttag gaacaaaacc
     6721 tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc ccttctaaaa tagcagattc
     6781 tgctggttct accccgatta attcgatttt cggatacaca cgttttaatt ctcggccagc
     6841 gccagtgatc gttcctcctg taccgatacc tgcaacaaac gcgtccaagc catttactcc
     6901 aaatgcttga tgtatctcag gaccagtcgt cttctcatgc acaagaggat ttgcttcatt
     6961 ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc aaacgttctg cttctctgat
     7021 tgatccagaa ataccatccg caccaggagt caaaatcaac tctgcgccgt accctttcat
     7081 caataaacgt ctttcgatac tcatcgtctc aggcatcacg atgattactt tatatccttt
     7141 tgcaactcct accatcgaca atccgattcc tgtatttcca gaagtaggtt caatgatagt
     7201 atcacctggt ttcagtagac catcgtgttc tgctttttct atcatactta aagcaatacg
     7261 atcttttaca cttcctcctg gattaaaaaa ctcaagcttt acaaaaacat ctgctgaatc
     7321 ttctggtacg attttattta atttgacgat tggcgttttg ccaatcaatt cggtgactga
     7381 gttatagata gctgtcatct tcaattcctc cttgtctttg ctcttcttac ttatcataat
     7441 acaatctgtt catttatggc gagtgattcg tctttgacta gcaaaaaaaa gacaaagata
     7501 taccatatat ggtaatt
//
LOCUS       DACDCV010000113         6969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDCV010000113 DACDCV010000000
VERSION     DACDCV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.114"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV041_002500"
     CDS             complement(<1..173)
                     /locus_tag="IV041_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8383243.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGI"
     gene            complement(186..1466)
                     /locus_tag="IV041_002501"
     CDS             complement(186..1466)
                     /locus_tag="IV041_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8383244.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(1607..2209)
                     /locus_tag="IV041_002502"
     CDS             complement(1607..2209)
                     /locus_tag="IV041_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8383245.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2454..2660
                     /locus_tag="IV041_002503"
     CDS             2454..2660
                     /locus_tag="IV041_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383246.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2972..3691
                     /locus_tag="IV041_002504"
     CDS             2972..3691
                     /locus_tag="IV041_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8383247.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(3772..4455)
                     /locus_tag="IV041_002505"
     CDS             complement(3772..4455)
                     /locus_tag="IV041_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8383248.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4673..6859
                     /locus_tag="IV041_002506"
     CDS             4673..6859
                     /locus_tag="IV041_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8383249.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2551 a         1172 c         1311 g         1935 t
ORIGIN      
        1 taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc cggatcagag
       61 gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt gatcaccgtt
      121 gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat catatcatta
      181 ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc aattgatgtt
      241 tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa gccaaggcct
      301 tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt taacggtaat
      361 gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt gagtatggtc
      421 aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc caagataata
      481 ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg ctcaggaaaa
      541 tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga cagtaaacta
      601 cggatctcag gaactttcat attatccaac atacttagat tggccggaaa aaggttcatc
      661 atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc taagtcgcct
      721 tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt gaattctctc
      781 aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct gacttgttgc
      841 tctaataact gatcagctaa tttcttatta gagattttcc aataataaac caagatgatc
      901 aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac atcaggtgtt
      961 tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat caatgtcccg
     1021 atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga tttttttaaa
     1081 caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata ctgcaatgaa
     1141 tctcctggtt caataaccga agatttcaaa acaaaaaaat cgtcgatgct tgttaagtga
     1201 tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg ttgacgcgaa
     1261 gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc agtaaaaatc
     1321 cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac tcccgcaaac
     1381 agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta aatcaaatat
     1441 tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt accaaattat
     1501 agcacaatta gcgtattatt tttttaattt ttcagggatt ttgaattcag aactcatacc
     1561 aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa tgattgtttt
     1621 tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc aacgtagcta
     1681 tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag aaaaataaag
     1741 taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg ggacgtttct
     1801 cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt aacaaaaagc
     1861 agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga tgtttcatca
     1921 ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat tcagagtcgg
     1981 aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg attcctgttt
     2041 ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt ccaagattat
     2101 tcaataaaac ttctaaggca aattttactt ggacatacac catatcttct tccgatttgt
     2161 ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc tcatacgtac
     2221 ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca taaaaagtgg
     2281 ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa gaaccttacc
     2341 aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt gctttttcga
     2401 gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt cttatggaaa
     2461 atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta tttgaatcac
     2521 caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt gtcatgaaat
     2581 gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt gtacgcaaat
     2641 caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag gttgtgagtc
     2701 tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca tgttttatga
     2761 atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa aaagggctgc
     2821 gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg gctactttta
     2881 gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa atgaagtagt
     2941 aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca ttgttgcagg
     3001 aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt tagaaacaaa
     3061 acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt ctaatccaga
     3121 aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg caatcaaaga
     3181 tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata gtatgctgtt
     3241 tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt atgaaggtct
     3301 tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac cagatctact
     3361 ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa gaggccggga
     3421 atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac tcaatcgacg
     3481 atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg atggggacaa
     3541 atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa tcaagatgcg
     3601 ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta caaataaccc
     3661 gaaagcaaag ggaatcttta caaataaata atataaaaac taagcaaatt gctcactcgc
     3721 agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag atcaaatagt
     3781 gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg ccatgatcaa
     3841 aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac agaaaacaag
     3901 gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa atcgatcaaa
     3961 atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc cttttaaatt
     4021 tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag ctacccactg
     4081 gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat ggacgggtgt
     4141 caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc gccagaaatg
     4201 tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc ctaagaaaaa
     4261 gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa atgcttggat
     4321 gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt ggatataagt
     4381 ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac gcccctcgtg
     4441 aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc cttatcttag
     4501 cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc gcttcccatt
     4561 ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac tttaaataac
     4621 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
     4681 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
     4741 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
     4801 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
     4861 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
     4921 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
     4981 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
     5041 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
     5101 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
     5161 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
     5221 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
     5281 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
     5341 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
     5401 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
     5461 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
     5521 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
     5581 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
     5641 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
     5701 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
     5761 gactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
     5821 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
     5881 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
     5941 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
     6001 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
     6061 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
     6121 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
     6181 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
     6241 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcgaa
     6301 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
     6361 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
     6421 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
     6481 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
     6541 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
     6601 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
     6661 aaagaaagta atcgacttta atgaaataaa agcagaacca gtgacgacga tagaagaaga
     6721 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
     6781 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
     6841 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
     6901 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
     6961 gaagctatt
//
LOCUS       DACDCV010000114         6671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDCV010000114 DACDCV010000000
VERSION     DACDCV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.115"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            187..2862
                     /locus_tag="IV041_002507"
     CDS             187..2862
                     /locus_tag="IV041_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8383250.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3044..3283
                     /locus_tag="IV041_002508"
     CDS             3044..3283
                     /locus_tag="IV041_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383251.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3299..4672
                     /locus_tag="IV041_002509"
     CDS             3299..4672
                     /locus_tag="IV041_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383252.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4676..6310
                     /locus_tag="IV041_002510"
     CDS             4676..6310
                     /locus_tag="IV041_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383253.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6473..>6671
                     /locus_tag="IV041_002511"
     CDS             6473..>6671
                     /locus_tag="IV041_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383254.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2343 a         1072 c         1337 g         1919 t
ORIGIN      
        1 ataatttaat aaggattgat ttatcgtaaa agaaaaaagt tgtgatttcc aaaatcacaa
       61 cttttttctt ttgtcaagat tttgagtggg atatattttt gtagaactga agatcatgaa
      121 attatgagag gttggaggta atgtagtcat acaaataaaa attttttaga aagtgagtgg
      181 taattaatga aaatgaatca cacttcaatt cagaagcatt tacaactgtt aaatacactg
      241 gggatttctt atgttaattt gagaactttt tctacattaa acaagaaaac cgtttctcat
      301 attttttcta ctgcagattc tcaagcaatt attaactatt tagaaaaaca gaaattgtct
      361 gaaggtacag taaacataac ttataatcct attaaacaag aggtgattac acaaaaaaag
      421 cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga tattgaccct
      481 aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa
      541 caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt tgatagtggg
      601 aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga gacgattctt
      661 actattcaga attttcttca attacttcac cgaagatttg gagtagaaga tgaagttgat
      721 attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac accagcggtc
      781 aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc tattcccgat
      841 aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga aaattccatg
      901 atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc ttttattcaa
      961 gcagactata aacaatggtt agctcactat cctgaattaa cttataccat aaaagagggc
     1021 gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa acacacgaat
     1081 aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt tcattgtctg
     1141 catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc gttgccaaaa
     1201 cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt tgaccagttt
     1261 aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa agttgcttgt
     1321 ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac acttgttctt
     1381 gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg tttacaaact
     1441 aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag agaagctgaa
     1501 gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga acatgaatct
     1561 cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc ttttggacgt
     1621 gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa tggtacagaa
     1681 gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga acttgcttgg
     1741 tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc taatactttt
     1801 ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac tacgttacac
     1861 tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa tgctaccaat
     1921 aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat actagatgag
     1981 ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc ttcaggagaa
     2041 gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt ttctgttttg
     2101 acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt aactggtttg
     2161 aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa tcacgcagag
     2221 aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt tcttttgaaa
     2281 ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca aaaaaaacta
     2341 tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct agctttgatt
     2401 tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt agagcatata
     2461 cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt aggaagtgag
     2521 caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa atttttaaca
     2581 aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat tcaaaaaaat
     2641 ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag gcgagaattt
     2701 caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa tatgtgtgca
     2761 gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc aacatacgag
     2821 attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat aaaccaaagt
     2881 atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa acaactgatt
     2941 acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg atctatgctt
     3001 catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta aaaataaaat
     3061 gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc aagccttttt
     3121 tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat ctatcggggg
     3181 tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc aacgttcgtt
     3241 caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg aatcaagcat
     3301 gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta gtaatgacgc
     3361 tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc cccaacgatt
     3421 ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac agcttagagc
     3481 ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg atcgattggc
     3541 acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc aggttcaagt
     3601 gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga ataaactatt
     3661 tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg agaatgttcg
     3721 tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg taccatatgg
     3781 gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga ttgttcgcta
     3841 tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc aacgattgac
     3901 gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt atagtatcct
     3961 aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt atctaggaac
     4021 ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc gccaatcaag
     4081 gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc ggtgtccctt
     4141 ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga agacgcttca
     4201 ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag aaagggttga
     4261 gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct atcaagcctt
     4321 gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac gcaaagtaat
     4381 ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca attgttcact
     4441 ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt cttcgtttct
     4501 ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag acctctctag
     4561 tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata aaacagtttg
     4621 tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat gatctatgga
     4681 aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag aacaagcttt
     4741 agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg aacggatggg
     4801 ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca caaaaaatcg
     4861 gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc tactagtgat
     4921 ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag tcgaggaatt
     4981 attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga ccacttcgac
     5041 aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca accaaattgc
     5101 ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt atgcaggagg
     5161 ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat tggtcattga
     5221 accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta
     5281 tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt
     5341 ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt atttagaata
     5401 tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga gaccgatttt
     5461 agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag tacaagaacg
     5521 ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg tattgacagg
     5581 cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca caaataccct
     5641 aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc ggaataaagg
     5701 agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat ttgtcgccga
     5761 ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg cagcgttaaa
     5821 tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg
     5881 aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag atagtcctga
     5941 attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac gacagttgca
     6001 cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc aagtcaaaga
     6061 tgtccaacgg atattgacta atttggatcg ttttcttgcc cagagtgaga agaagcaaat
     6121 caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc ataaggagat
     6181 gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg agcaatatca
     6241 aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac cctttacgtt
     6301 aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct tctctagcga
     6361 tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga tatttattaa
     6421 tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag atatgaaaaa
     6481 gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac atgtaccacc
     6541 taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag atgatttcct
     6601 taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa
     6661 tgtttattat a
//
LOCUS       DACDCV010000115         6541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDCV010000115 DACDCV010000000
VERSION     DACDCV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.116"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(149..670)
                     /locus_tag="IV041_002512"
     CDS             complement(149..670)
                     /locus_tag="IV041_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8383255.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(672..1706)
                     /gene="sppA"
                     /locus_tag="IV041_002513"
     CDS             complement(672..1706)
                     /gene="sppA"
                     /locus_tag="IV041_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8383256.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1739..3154)
                     /locus_tag="IV041_002514"
     CDS             complement(1739..3154)
                     /locus_tag="IV041_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8383257.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3123..5276)
                     /locus_tag="IV041_002515"
     CDS             complement(3123..5276)
                     /locus_tag="IV041_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8383258.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5534..5770
                     /locus_tag="IV041_002516"
     CDS             5534..5770
                     /locus_tag="IV041_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8383259.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5789..6004
                     /locus_tag="IV041_002517"
     CDS             5789..6004
                     /locus_tag="IV041_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8383260.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         2063 a         1184 c          985 g         2309 t
ORIGIN      
        1 tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat agaagataga
       61 aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc actaattaaa
      121 atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta agtgttttag
      181 aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca accactgacg
      241 tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc accaaatacc
      301 caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga ataaagatag
      361 tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa atcattttcc
      421 caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa taaatagcta
      481 atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc atgattttgt
      541 aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca ataagaaaag
      601 caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca atgccactct
      661 tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc agaaaatcca
      721 agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc ttgccattca
      781 gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt atattctata
      841 accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc ttcagagtaa
      901 ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat tcttccatcc
      961 gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac attgacaaaa
     1021 cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc tgaaggtttg
     1081 tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc agttacgcca
     1141 agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat tgatccagtt
     1201 gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta ccctccactt
     1261 gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat ttttgccatt
     1321 tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac ttctaataac
     1381 actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa attctgatgg
     1441 ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac tccattgacg
     1501 gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc tgtgatttcg
     1561 ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc ttcctcttta
     1621 tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca cgctactccc
     1681 acagcaatcc atcttctttt attcatataa actcctccta tattcttagt aaaaataatt
     1741 attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct cctacaatta
     1801 aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt tttaacgttc
     1861 ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa atttcatcta
     1921 aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa tgaaccttca
     1981 tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat ttcatttttg
     2041 atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt tctgcttgtc
     2101 cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt attccttttg
     2161 aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt tcatttagct
     2221 ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt cctttttccg
     2281 tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct ttttctgact
     2341 gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt gctgaaattg
     2401 tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct tgccaattct
     2461 catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta ctccaagcta
     2521 cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt ttttctagtt
     2581 gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca ctttgcttca
     2641 aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta ctgtagccaa
     2701 aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata aatagctgag
     2761 ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc ttttcattct
     2821 ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat tgattttctt
     2881 ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct attttttcgg
     2941 ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt aaaaacaatc
     3001 caataataca taaaaaaaga attacagggt ataacaccca acggtagaag ctatgatgtt
     3061 gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat tgtttccctc
     3121 tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc atcgtgcgtt
     3181 cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc ttttgctatt
     3241 ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag ataattcata
     3301 attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat taatatgtcc
     3361 ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc tgaaagatta
     3421 tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat aaattcattt
     3481 agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct atctaaaccg
     3541 aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg caaatatgtc
     3601 actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc ataatggatc
     3661 gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga ttttccagaa
     3721 ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga aatttcgttc
     3781 aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat atcttgttga
     3841 aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga atcaatcgcg
     3901 aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac ttgtaaatta
     3961 ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt gataagttga
     4021 cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat taatgcactg
     4081 ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt aaccgtacga
     4141 aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac tttctcttca
     4201 ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact agaatttaca
     4261 gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac gaaagcgaat
     4321 attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt actattctga
     4381 atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat tgataacgta
     4441 gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat aatttcccct
     4501 gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa atactgaagc
     4561 ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg actatattca
     4621 aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt taaagttgaa
     4681 cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga gcttcctatg
     4741 ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc tttttgtttc
     4801 caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg aatataagtt
     4861 tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc ttcaatcgtt
     4921 tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt cgttttataa
     4981 attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa tggtaagata
     5041 agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc aaaagacaat
     5101 ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt cccagatagg
     5161 tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat cgaaaggcaa
     5221 gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt tatcacttat
     5281 ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc tttaatacag
     5341 tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa cactaacttt
     5401 ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa cttgataagt
     5461 ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg taaaaaatat
     5521 tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt gtggaaatgc
     5581 acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg taggtggact
     5641 taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac cagcttgcgg
     5701 tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct gggatgctcg
     5761 aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa caacatttat
     5821 attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag ttttaggcac
     5881 tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac cttatgcaat
     5941 agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt ggaccggtgc
     6001 gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg aacataggta
     6061 tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag cttcttgtgc
     6121 tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa aattctttgg
     6181 aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag
     6241 gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga tgtgcattaa
     6301 agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca aatcctatca
     6361 agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa tgaaaaacac
     6421 tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac atttaatttt
     6481 aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg gccctttttt
     6541 g
//
LOCUS       DACDCV010000116         6457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDCV010000116 DACDCV010000000
VERSION     DACDCV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.117"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1110
                     /locus_tag="IV041_002518"
     CDS             103..1110
                     /locus_tag="IV041_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8383261.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1122..2126
                     /gene="neuB"
                     /locus_tag="IV041_002519"
     CDS             1122..2126
                     /gene="neuB"
                     /locus_tag="IV041_002519"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8383262.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2123..3271
                     /gene="neuC"
                     /locus_tag="IV041_002520"
     CDS             2123..3271
                     /gene="neuC"
                     /locus_tag="IV041_002520"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8383263.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3244..3921
                     /locus_tag="IV041_002521"
     CDS             3244..3921
                     /locus_tag="IV041_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8383264.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3911..5053
                     /locus_tag="IV041_002522"
     CDS             3911..5053
                     /locus_tag="IV041_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8383265.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5066..6043
                     /locus_tag="IV041_002523"
     CDS             5066..6043
                     /locus_tag="IV041_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8383266.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6043..>6457
                     /locus_tag="IV041_002524"
     CDS             6043..>6457
                     /locus_tag="IV041_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8383267.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRA"
BASE COUNT         2409 a          808 c         1232 g         2008 t
ORIGIN      
        1 cgttacgttt tgacatcatt attgataatt agtttagtta tatatttttt aaagattaag
       61 aatcgaatta tagatgacta taattcatag gaggagatat aagtgtttaa aggaaaaaca
      121 atattagtaa ctggcggaac tggttccatt ggaagtgaaa ttgttcgtca gcttttaaaa
      181 tatgaaccaa aagctatcag aatttatagt agaggtgaag aaaaacaatt ttatatgcaa
      241 caagagctct caaatcacac taatttgaga tttttaattg gtgatatcag agactctcaa
      301 aggattgatt atgcatgtcg tgatgtggat attatttttc atgctgccgc aatgaaacac
      361 gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa caaatgtgat gggaactcaa
      421 aatgtaatag atgcagcgat caaaaataat gtaaaacatt ttgtaggtat cagtacagat
      481 aaagtagtga gtcctactaa caccatgggt gcgacaaagt tattgagtga aaaattaata
      541 atagctgcca atcagtataa aggaaaaaca gaaacaatat tttcttgtgt aaggtttgga
      601 aatgtcatgg ggtcaagtgg atctgttatt cctcttttta gtaaacaaat aaaaaacaat
      661 aaagatatta ctttgacaga tgttgatatg acacgattta tgatgtcaat accggatgct
      721 gcaagattag ttctgtctgc tgctatgaat tcaaaatttg gagaaacata tgtcttaaaa
      781 atgccggcaa tcaatatttt aactttagct caatcattaa taaaaatata taatgataca
      841 catgatgaaa aatatagagg tgaaataaaa gttgtaggta tcaggccagg agaaaaatta
      901 tatgaagagc taatgactac agacgaaatt gaacgctcat atgaaaatga taaaatgtat
      961 atcattccac cacactatca tgctactgac gaactgtaca aagattatga aaaaattaaa
     1021 atcactgcat actcttcaga tgcaacagaa ttaatgagtg gacaggaatt tattagaatc
     1081 gcaaaagaga ataatgtcgt gacttggtaa ggagttttat gatgaaatca gaagtatatg
     1141 taattgcaga aattggtgta aaccataatg gaagtctaga tttagcttta gaaagtattg
     1201 ataaagctaa agaatgtgga gctaacgcgg ttaaatttca aacatttaaa acgaataagc
     1261 tagtttccaa aaaggctcca atggcaaaat atcagaagga taatcttaaa gaggacagtt
     1321 ctcaatttga aatgctaaaa aaattagaat tatctcaatc tgattttatt agaataaaag
     1381 attattgtat acaaaataaa attgattttt tgtctactcc ttttgatgaa gaaagtgcag
     1441 actttctaaa acaaattggt gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt
     1501 taccttttct tagaaaaata gatatgtatg ggttgccagt tttattatct acaggtatgg
     1561 ctgatattca tgaggtcagt gaggctgtag attgtttcat agatagtcca gttactattt
     1621 tacattgtac ttcaaattat ccggctagtt ctgaagatat taatttattg gcgattaaca
     1681 cattaaaaga aatgttcggg atacccgttg ggtactccga tcattcattg ggatatgatg
     1741 ttggtatttg tgctgttgcg atgggtgcta aggtaattga gaaacatttt accttggata
     1801 aggaactacc tggtccagat cataaagctt ctttagatcc tcaggaattt gcagattttg
     1861 taaagcatat tagaaacact gaaatatttt taggtgatgg tgttaagcga gcaatgcctt
     1921 ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt ggtcactgag aatttaaatg
     1981 tgggagatat tcttacagag aaatctctta caataaaacg tcctggaaat ggaatagctc
     2041 ctaagttttt atatcaatat attggcaaaa aagttaaaag accaataagt gcagatagtg
     2101 ttctgcttga ggaagatgtt gaatgaaaaa aatacttata gtaagtggaa cacgagctga
     2161 ttatggaata tataaacctg ttgctgagaa tttactacaa gataattggg atgtatcatt
     2221 tgttgtttct ggaatgcatt tatcccataa atatggattt actaaagaat tgatcttagc
     2281 tgacggattt agaattagtg gagaagttca aagtttgttt ttagagaaca ataagggaaa
     2341 catggctcgg tctgtggcat tagagataaa tggtttcaca aatatatttg aacaaactag
     2401 ccctgatttt gtattattac taggggatag aggagaaatg ttggcagctg cgatagcttg
     2461 tttgtatctt ggaatcaaaa cagcacatat tcacggaggc gaagtgtctg gtactgtaga
     2521 tgaatcaata agacatgcca taacgaaaat agcatctgtt cattttgctg ctacggaaaa
     2581 aagcaaagag cgtttaataa aaatggggga agattcttgg agagtttttc ccgtcggagc
     2641 tcctagaata gatactattt taaataatag attacctagt tttagagata taaaagaaaa
     2701 atatcatcta aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc cagttgtaac
     2761 cgaactagaa aatatagaaa aacaaatttt agctttgatc aattcaatgg aacaacaatc
     2821 tcaaccaatt atttgcattt tacctaatag tgatgctggt agcgaaatta tcagaaaaat
     2881 gtaccagaaa tatttaaaaa cggatattat ttatattacg aatttagagc cggaggaata
     2941 cctgactata ttaaaagagt gcaaatttat gatcggaaat tcttcttctg ggataattga
     3001 agctgcatca ttcaaaatac cagtaataaa tttaggaacc agacaaaatg gacgagaaca
     3061 atctaaaaat attataaatg caaatctaaa tgaaaatgaa attgaaactg ctttgaataa
     3121 aatacaagat cctcagttta taaaaagctt gaaaaatgtt aagaacatct acggtgacgg
     3181 caacagttct agaagaataa caaaaacatt gaatcaaata ttgaatgatg gggaagatat
     3241 taagtggatt caaaaacaaa tagcttatta aaatatacta gtgatccata cattattatt
     3301 ggggctggcg gacacgcaaa agtcattatt gaaaccttaa gattagaaaa taagttaata
     3361 tatggatttt ttgatgataa gcaaaataaa atgcaagatc tgaaatatct tgggaaaata
     3421 aacgaattga atagctgggc ggatagcaaa aaaaaaatct ataaatttat tgtagctatt
     3481 ggtgactgtg tagtaagaaa aaagattata aaaaaattga atatgccaag tgagcaatat
     3541 ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg ttatggaact
     3601 gttatttttg cgaatgctgt aattaaccca gatgctgtaa ttggagagca tgctataata
     3661 aacacaggta gcattgttga gcatgattgt agaattaata actatgttca tttatctcca
     3721 ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg aattggttct
     3781 caatgtatcc agatgaagaa aattggatct tggtgtatga taggcgcagg gagcactatt
     3841 gttaaggata ttccctcaca ttccttagct tatggcaatc cagctaaaat taagaaagaa
     3901 ggaattaatt ttgaattcta gaattttatt atcaagtcct catatgggag gaacagaaga
     3961 aaaatatgtt gctaaggctt ttaaagataa ttggatagcg ccattaggtg aaaatgtgga
     4021 taaatttgaa gaaagtatta caaaatatac tggttctaag ggcggagctt ctgcgttaag
     4081 ttcaggtaca gctgccatac atcttgcctt aagactgctt aatgttggaa aaaacgatat
     4141 tgtctttgct ccttcattta cttttatagc tacagtaaat cctattttat atcaaggggc
     4201 tgtaccagtg ttcattgact cagaacctaa tacatggaat atgagccctg ttgcactaga
     4261 gagcgcatta gaagaagctg aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa
     4321 tttatatggt caaatggctg attatgaacg aattttagct attgcaaacc aatttaatgt
     4381 tccagtgata gaagatgcag cggaatcgtt aggttctact tataagggaa tttcgtcagg
     4441 cttatttggg acaattggga tctattcatt taacggtaat aagatcatca caacctcagg
     4501 tggaggagct ctagtgtcta gtgataaaaa atatgttgag cattcaagat ttttagcaac
     4561 acaagcaaga gatcaagcac cgtattatca acatagcgaa ataggttata attaccgtct
     4621 aagcaatatt tctgccggta tcggacgtgg acaaatggaa gtattgaatg atagagtagc
     4681 taaacgaaga gaaattttca atcgatataa ggaagctttt tctggaattg atagaattga
     4741 aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa
     4801 tgattataat tctattgatc aagtagttaa cgtaataaat aaactaaatg aaaaaaacat
     4861 tgaaacaaga gctttgtgga aaccactaca tttgcaacca gtatttcaag gggtaaaaat
     4921 gtattaccat gataataatc ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt
     4981 accttctgga agtaatatga cagatagtga acaagaattt gttattgatg agtttaaaaa
     5041 aatattcaag taggtgtgat attttatgaa cataggaatt attggtttag gttccatggg
     5101 gaaaagaaga cttagactca ttcaagaaca ctttaatgat attttactat gtggtgttga
     5161 tactaatgag gcgagacgaa aggaagttga aaaggaattt ggaataccaa cttataatag
     5221 tctaacagag gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta catcccctat
     5281 aacccatgaa tttattataa aagaggcttt aaatttagat tgtaatatct ttacggaaat
     5341 caatttatta aatgaatatt atgatgaaat aatcgaagta gctaaaaaga aagatagatt
     5401 actttatcta tcttctacat ttttgaagag aaaagaaatt caatatatta agtctaaagt
     5461 gacgcataat aaaaaaattt cttatcaata ccatgtagga cagtatttac ccgactggca
     5521 tccttgggaa gattatcgca atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga
     5581 aatattcgct attgaattac cttggattat agatacattt gggaaagtta aatcgtttac
     5641 tgtaatgacc cagaaattat ccaatcttga tattgattat tcggacagtt tcactcttat
     5701 tattgagcat gaaactggta tagttggaac tttaagtgta gatgttgttt caagagtagc
     5761 taaaagaaat ttaactataa ttggtgaaaa gattttaata gaatgggaag gtaacccgga
     5821 ttctctattt tcatggaatg aagaaacttc tagtatgaca gatattcaat tgtacgatga
     5881 ggttatgcat gataataatt attcacatac tattattgaa gatgcatatt tagaggagat
     5941 taaagaattt ttcacgcttt tggaaaagaa aatggaaccc caatatactt ttgaaaaaga
     6001 taagtatatt attaacttaa ttaacgacat tgaggtaaat taatgatgaa agtaattttt
     6061 tttgggttag ggtcaattgg aaagagacat ctaaaaaatc taataagcta tggaaaaaag
     6121 aattctatta attttgatat aacagcttat agtagcagtg gaaaaaaaga gtttgaagaa
     6181 ataacgtata ttcaaaatag agacgatttg gatacacatt atgatatagc ttttattaca
     6241 aatccgactt ttaagcatct ggaaacacta aatgtaattg ttgaaaaagt tgattacata
     6301 tttttagaaa aacctgcttt ttctgagagt attgatctta ctcaatataa ttatgattac
     6361 aaaaatattt atgttgctgc tccactgagg tataaaaata tcatggaagt tgttcgtaat
     6421 agtattcaaa gtaaaaaagt atattctgta agagcta
//
LOCUS       DACDCV010000117         6287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDCV010000117 DACDCV010000000
VERSION     DACDCV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.118"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IV041_002525"
     CDS             <1..177
                     /locus_tag="IV041_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383268.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IV041_002526"
     CDS             190..690
                     /locus_tag="IV041_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8383269.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IV041_002527"
     CDS             787..2634
                     /locus_tag="IV041_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8383270.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IV041_002528"
     CDS             2637..3533
                     /locus_tag="IV041_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8383271.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IV041_002529"
     CDS             3583..3972
                     /locus_tag="IV041_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383272.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="IV041_002530"
     CDS             3959..>6287
                     /locus_tag="IV041_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8383273.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DACDCV010000118         6282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDCV010000118 DACDCV010000000
VERSION     DACDCV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.119"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..945)
                     /locus_tag="IV041_002531"
     CDS             complement(<1..945)
                     /locus_tag="IV041_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8383274.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISIL"
     gene            complement(947..2386)
                     /locus_tag="IV041_002532"
     CDS             complement(947..2386)
                     /locus_tag="IV041_002532"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8383275.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2571..4517
                     /locus_tag="IV041_002533"
     CDS             2571..4517
                     /locus_tag="IV041_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8383276.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4781..5653
                     /locus_tag="IV041_002534"
     CDS             4781..5653
                     /locus_tag="IV041_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8383277.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            5949..>6282
                     /locus_tag="IV041_002535"
     CDS             5949..>6282
                     /locus_tag="IV041_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383278.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT         1998 a         1097 c         1125 g         2062 t
ORIGIN      
        1 aagtattgag attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag
       61 aagaatatca acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg
      121 tgggaaaaat ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg
      181 gatattaagt ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga
      241 aacaaaaata ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg
      301 gatagtagac agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa
      361 acctagtgca cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt
      421 attttgacaa ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc
      481 tgaagaaaca atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct
      541 cacacgctca taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg
      601 gttggcttca agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca
      661 aataatcgtt ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga
      721 gtaaaagata ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg
      781 aagtccacgt gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt
      841 ttgttttgtt ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg
      901 gctaactcca gctaattcag cgacatcagt taattttgct accattttaa tattttaatt
      961 caaagtattt tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag
     1021 ggaatacacg actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt
     1081 tatctacaaa aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac
     1141 caaagtcagt ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt
     1201 tggtatctac aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg
     1261 atttctgatt tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg
     1321 ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa
     1381 gtttaccatc ttttattgaa agttctttta caagactcat tgctccctga taatcatact
     1441 tatcagttgg ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat
     1501 caggagtatt gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt
     1561 ctgaagcgcc cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat
     1621 tcggatagat attcccataa tttagctcag atttatccaa cccttgagga caataaatga
     1681 gaacaggttt tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag
     1741 tgccgccaaa atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt
     1801 tgtaaagttt gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt
     1861 gtccttcata attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat
     1921 gaataagaac attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt
     1981 gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat
     2041 tcccctctat ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg
     2101 gaagaagttc tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc
     2161 attctttcaa cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc
     2221 cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat
     2281 agctagcacg ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag
     2341 tccaatcaga ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca
     2401 atttatcttt ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt
     2461 taaaaaaata aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt
     2521 tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca
     2581 gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac
     2641 actgcgctac acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt
     2701 tagacgaaaa tccagatgtg aaaagcacat ttaaaattaa tggacaatat caagtgatta
     2761 tcggtgcagg tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg
     2821 aaatttcaac agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta
     2881 tggcattaat taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag
     2941 gtggtttatt aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat
     3001 caattgaaga aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg
     3061 ctgcaccatt catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag
     3121 caaaccaatt tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct
     3181 caactgaata ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag
     3241 ttattccagt tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa
     3301 aattgccatc atctattgac tttacattca cgccattatt atcagtaatg attactggat
     3361 ttttgacttt cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca
     3421 ttattgtttg gctatataac actactggat ttattggaat gggtattttt ggaggaacat
     3481 attcacttat agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac
     3541 tttcagcgtg gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg
     3601 caaacgttgc tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga
     3661 caaaaagtct tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct
     3721 tgttcggagt taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa
     3781 ttgctacagc agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt
     3841 tcttgggatt tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga
     3901 ttagctttgt agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta
     3961 tctttaaagc agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa
     4021 cagatccaat atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg
     4081 ttagtcccat ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat
     4141 cagaagctat gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag
     4201 tagatggtac tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata
     4261 atggtgctga agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat
     4321 ttacacaaaa agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg
     4381 accgttcaat gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata
     4441 cttcggatta ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt
     4501 tacttgaaat caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca
     4561 aataatatag attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca
     4621 attgtatgaa aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt
     4681 agttatttac ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt
     4741 tatttagtga tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga
     4801 agcaggcggc acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag
     4861 agtgagcttt ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa
     4921 acaataccaa gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca
     4981 tcgcgattcc gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt
     5041 tgatttcatc ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt
     5101 caatgcggct gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt
     5161 ttactatacg atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga
     5221 agggtttagt catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt
     5281 tgctggcagc tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat
     5341 cgaaaaacgc ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat
     5401 cgaagcctcc tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt
     5461 gatcattttt ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc
     5521 atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac
     5581 cccagcatta gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt
     5641 cttacattca taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa
     5701 ctgaaaattt agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga
     5761 agatcaagcc ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt
     5821 aggccacttg tattgtgcaa tagtttttta cacgccgttt gtataattaa gcgagacaaa
     5881 taaaaaagcc atttatgtta cactcaaatc gtaatacccg ctaaagcata caaggagagt
     5941 gaacataaat gacttatacc catcttacac caaacgagct tgtaatgata gaagcatatt
     6001 ttcatcaaga aactccggtt gctatcgttg cgaagcagct taaacgtgga cgccaaacaa
     6061 tttacaatgt ctataacttt ctcaaatgtg gtggaacagc acttgaatgc tttgaacaat
     6121 acaaagaaaa taagcgacgt tgtgggagaa ccgaaatcat ttttcctgct gaggaaaaag
     6181 aatacattgc aaaaagatca actgaaggtt ggaccccaga cgtcattatt ggccgtgcag
     6241 agcgaacctt ctcttgttca gtaagtaccc tctatcgccg gt
//
LOCUS       DACDCV010000119         6069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDCV010000119 DACDCV010000000
VERSION     DACDCV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.120"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..1451
                     /locus_tag="IV041_002536"
     CDS             39..1451
                     /locus_tag="IV041_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8383279.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="IV041_002537"
     CDS             1800..2432
                     /locus_tag="IV041_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8383280.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="IV041_002538"
     CDS             3020..4459
                     /locus_tag="IV041_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8383281.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="IV041_002539"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="IV041_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5221..5436
                     /locus_tag="IV041_002540"
     CDS             5221..5436
                     /locus_tag="IV041_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383282.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="IV041_002541"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="IV041_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1999 a          976 c         1238 g         1856 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataact
//
LOCUS       DACDCV010000120         6003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDCV010000120 DACDCV010000000
VERSION     DACDCV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.121"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IV041_002542"
     CDS             complement(<1..287)
                     /locus_tag="IV041_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8383283.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="IV041_002543"
     CDS             429..1547
                     /locus_tag="IV041_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8383284.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="IV041_002544"
     CDS             1675..3051
                     /locus_tag="IV041_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8383285.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="IV041_002545"
     CDS             3452..5032
                     /locus_tag="IV041_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8383286.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="IV041_002546"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="IV041_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="IV041_002547"
     CDS             complement(5658..5978)
                     /locus_tag="IV041_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8383287.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         1971 a          991 c         1223 g         1818 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttt
//
LOCUS       DACDCV010000121         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDCV010000121 DACDCV010000000
VERSION     DACDCV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.122"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV041_002548"
     CDS             <1..395
                     /locus_tag="IV041_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383288.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IV041_002549"
     CDS             398..1228
                     /locus_tag="IV041_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8383289.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IV041_002550"
     CDS             1244..2095
                     /locus_tag="IV041_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8383290.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IV041_002551"
     CDS             2092..2451
                     /locus_tag="IV041_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8383291.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IV041_002552"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IV041_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IV041_002553"
     CDS             2624..2929
                     /locus_tag="IV041_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383292.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IV041_002554"
     CDS             2929..3240
                     /locus_tag="IV041_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383293.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IV041_002555"
     CDS             3407..3823
                     /locus_tag="IV041_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8383294.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IV041_002556"
     CDS             3823..4491
                     /locus_tag="IV041_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8383295.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IV041_002557"
     CDS             4488..4865
                     /locus_tag="IV041_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383296.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IV041_002558"
     CDS             4862..5161
                     /locus_tag="IV041_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383297.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IV041_002559"
     CDS             5403..5819
                     /locus_tag="IV041_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8383298.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDCV010000122         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDCV010000122 DACDCV010000000
VERSION     DACDCV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.123"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IV041_002560"
     CDS             complement(<1..985)
                     /locus_tag="IV041_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP8383299.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IV041_002561"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IV041_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8383300.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IV041_002562"
     CDS             1810..2964
                     /locus_tag="IV041_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8383301.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IV041_002563"
     CDS             3179..4147
                     /locus_tag="IV041_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8383302.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IV041_002564"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IV041_002564"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8383303.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IV041_002565"
     CDS             5177..5785
                     /locus_tag="IV041_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8383304.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDCV010000123         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDCV010000123 DACDCV010000000
VERSION     DACDCV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.124"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IV041_002566"
     CDS             450..1604
                     /locus_tag="IV041_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8383305.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IV041_002567"
     CDS             1598..2020
                     /locus_tag="IV041_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383306.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IV041_002568"
     CDS             3143..4075
                     /locus_tag="IV041_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8383307.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IV041_002569"
     CDS             4092..4607
                     /locus_tag="IV041_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8383308.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IV041_002570"
     CDS             4903..5184
                     /locus_tag="IV041_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383309.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IV041_002571"
     CDS             5186..5602
                     /locus_tag="IV041_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383310.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDCV010000124         5640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDCV010000124 DACDCV010000000
VERSION     DACDCV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.125"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..1202
                     /locus_tag="IV041_002572"
     CDS             120..1202
                     /locus_tag="IV041_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8383311.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1202..2005
                     /locus_tag="IV041_002573"
     CDS             1202..2005
                     /locus_tag="IV041_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8383312.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2075..2815
                     /locus_tag="IV041_002574"
     CDS             2075..2815
                     /locus_tag="IV041_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8383313.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2826..3830
                     /locus_tag="IV041_002575"
     CDS             2826..3830
                     /locus_tag="IV041_002575"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8383314.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3875..5638)
                     /locus_tag="IV041_002576"
     CDS             complement(3875..5638)
                     /locus_tag="IV041_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383315.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         1817 a          932 c         1147 g         1744 t
ORIGIN      
        1 cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg
       61 aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt
      121 tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa
      181 atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt
      241 tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct
      301 ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat
      361 tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg
      421 aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg
      481 ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg
      541 gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc
      601 catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata
      661 atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc
      721 caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa
      781 caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag
      841 acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt
      901 tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag
      961 cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag
     1021 gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt
     1081 atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag
     1141 tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat
     1201 aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa
     1261 tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca
     1321 gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag
     1381 ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct
     1441 agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa
     1501 gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa
     1561 cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct
     1621 tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt
     1681 tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg
     1741 tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg
     1801 tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct
     1861 gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt
     1921 cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg
     1981 aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt
     2041 tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt
     2101 aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt
     2161 caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc
     2221 acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg
     2281 tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat
     2341 ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa
     2401 aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat
     2461 ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa
     2521 agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga
     2581 tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca
     2641 tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact
     2701 tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc
     2761 aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga
     2821 agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac
     2881 aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac
     2941 cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg
     3001 aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt
     3061 ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg
     3121 cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat
     3181 tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg
     3241 ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag
     3301 tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc
     3361 gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag
     3421 aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa
     3481 aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt
     3541 atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac
     3601 gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag
     3661 caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg
     3721 ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga
     3781 aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa
     3841 tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag
     3901 agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt
     3961 aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt
     4021 ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga
     4081 cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga
     4141 tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt
     4201 catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt
     4261 tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag
     4321 aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt
     4381 ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc
     4441 gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat
     4501 catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat
     4561 cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat
     4621 tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg
     4681 ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc
     4741 caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca
     4801 ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca
     4861 aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat
     4921 agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa
     4981 cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg
     5041 ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca
     5101 agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata
     5161 cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa
     5221 ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt
     5281 ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt
     5341 cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat
     5401 ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa
     5461 tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa
     5521 aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc
     5581 tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg
//
LOCUS       DACDCV010000125         5613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDCV010000125 DACDCV010000000
VERSION     DACDCV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.126"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..2009)
                     /locus_tag="IV041_002577"
     CDS             complement(60..2009)
                     /locus_tag="IV041_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8383316.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="IV041_002578"
     CDS             complement(2051..3109)
                     /locus_tag="IV041_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8383317.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="IV041_002579"
     CDS             complement(3228..3683)
                     /locus_tag="IV041_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383318.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="IV041_002580"
     CDS             complement(4267..5070)
                     /locus_tag="IV041_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8383319.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2075 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tat
//
LOCUS       DACDCV010000126         5246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDCV010000126 DACDCV010000000
VERSION     DACDCV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.128"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..743
                     /locus_tag="IV041_002581"
                     /pseudo
     CDS             71..743
                     /locus_tag="IV041_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            802..1242
                     /locus_tag="IV041_002582"
     CDS             802..1242
                     /locus_tag="IV041_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383320.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1254..1922
                     /locus_tag="IV041_002583"
     CDS             1254..1922
                     /locus_tag="IV041_002583"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8383321.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1986..2609
                     /locus_tag="IV041_002584"
     CDS             1986..2609
                     /locus_tag="IV041_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8383322.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2665..3051
                     /locus_tag="IV041_002585"
     CDS             2665..3051
                     /locus_tag="IV041_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8383323.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3264..4655
                     /locus_tag="IV041_002586"
     CDS             3264..4655
                     /locus_tag="IV041_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8383324.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1743 a          869 c         1098 g         1536 t
ORIGIN      
        1 tctactctaa gcatcagact ttcttttcaa aatatgctat tttaaaaatg atgaaaaaag
       61 ggggagaaaa atgaattttt tagttgtttt aaaagatact ctgattgagc gatctacatt
      121 tctatatgtt caattgcagg aaaataaaac attgctgcat ttttcaccag agttccatct
      181 agaggggcta acattaggag aaatctatca agcagaaggg ctgtcggctg ttttgcactt
      241 ttttaaggta gaattggaat taccagtaaa agattatatc gagctttctc ttgaatcttg
      301 ggatcgagca gtggttgaac tgtttccaga aggtttaatg atcgatacaa acgatgggaa
      361 gatccatctg actgcagcag aactgcaaaa acaaatgcgt tatcaaatag atgacgaaaa
      421 ttctttttct atttttagac gtcagcaaaa gattctaaaa agctttttaa aagtgatcag
      481 taaaaaaacg gaatctgatc aaaacgacac agctgttgaa aaaatatcca ggtctgcttc
      541 acacgtctat tcaatttcct caattgatta cgatgatcca tcgttttata cgtatgcaac
      601 agctaccatc taagaagctg tttcttccat tgacggacac atttcgagta gtcaacagag
      661 aagataaaaa gaaagtgatt gtgattgact ttaccagaaa tcggaatgtc cttcaccaag
      721 ttgcaatgga aaaggccaat taaggctttt tctttttgga tttttttgtc ataataaata
      781 ttagatgatg gaggagataa tatgatgcaa acatggttag aaaggctaat acgatttcag
      841 cagaactatc acaaacttat gcagggaaga tatgcgaaat ttgatcagtt gaatcgcggt
      901 ctattgatcg ctgcgcttgt ctttagtttg ctaagtcgtt ggttgcccta tagaatcgga
      961 caattgttgt tcatcgtctt tttcggatgg atgatatttc gtttcctatc aaaaaaaatc
     1021 taccccagac taaatgagaa tcaacgttat ttgaaatatc ttgatagagt caaagaaaaa
     1081 tatcatcaga taaaaaacaa aaagcaagtc aaaatggatc gaaaaagtta tacttttttt
     1141 gagtgcccga attgtcatca aaagcagcgt gcacctaaag gaaaaggacg aatacgtgtg
     1201 acatgcaaaa catgtggtat aaaattcgaa acgaatgttt aagggagaaa agaatgaaat
     1261 tagtattatt acgtcatggc gaaagcgaag caaattttga aaattactgg acaggatggc
     1321 tagatgtcgc cttaacagaa aaaggaaaag aacaagcagc agttgccggt caaaaaatgc
     1381 ttgaagccgg acttcaattc gatgccgttt atacctctgt tttgaaacgt gcgattctta
     1441 ccggacaagt cgcactagaa gaaatgggtc agttatggct tcctataata aaaacatggc
     1501 gtctaaatga acggcattat ggggatcttg tgggtaaaaa caaagataag atgaaagaaa
     1561 tatacggaaa agatcaagta aaaacatggc gcagaggata tacggaagtt ccgccgttaa
     1621 ccgcagaaaa tagatttgac cgcagatatg atcacttgga tccgcatttg atcccatacg
     1681 gagaaagtct ggaaatgaca gtgaatcgga tcattccctt atggcaagat catctagcac
     1741 caaaactaaa agatcggcaa gatttactag ttgtgggtca tggtaatagc atccgagcac
     1801 tgacaaaata tttagaagat gttcctgaag atcaaatgga tacgatcgat atcccaaatg
     1861 cacaaccaat ccagtataca ttagcagatg atttgacaat tctggacaaa aaaatacttt
     1921 aaagaacacc tttatctaaa agtgagagat agaaaaaaga agcaaaggaa tggaacaaaa
     1981 ggaacatgaa aaattatata tgggactttg atggtacatt gtttgatacg tatcccgcaa
     2041 tggtagatgg tgcatggcaa gcactaaaag attttggtat cagtatggac aaaaaagaaa
     2101 tctacttcaa aatgaaaaag tattctacta gttatttgat caatgaatca aacttgaatg
     2161 ccagagagtt taatgaatta tttcaccgat atgaaaaaga aagtacggaa gtttcccgtc
     2221 catttccaga aacgaaacaa gtactggaaa tgctaaaaga caacggcggt cgtcatttca
     2281 tcctgaccca ccgtttgaca gaatcaacat ggggcctgtt aaaagaacat cgattggcac
     2341 atctgattga ggaagtagta gggatcgatc aggactttcc tcgtaaacct gatccagcat
     2401 ctttaaatta tttgatcgat acttttcatt tagaaagaac agacacgatg atgatcggcg
     2461 atcgtcgttt agatatcgaa gcagggaaaa atgcaggagt cgctacttgt ctttacgaca
     2521 tcgatcattt tctgggtgag atcccggcag attatgtggt aggaaatctg aatgaaatcc
     2581 ttaccttgca gagcatcatt aaaaagtagt atcccctttc aaaggagata tgttataatt
     2641 ttcataaagg agtgataaaa ttatatggaa atcagaagac aaaaagaagt tgtagaagca
     2701 tttcattatg atatgagatt gccgaaccaa gaagtagaaa cagatttgaa agtaggcttc
     2761 tcgcctttaa aagaaacgat cgaaaactat ccgaaagaac atagcatcgt aggagcacgc
     2821 ttagagttcc gtttagtgtt tgatacatat gttttatctg gaagtgtgag tcagatcaat
     2881 catatcctca atcggaaaat tgaaggtcaa aatgatgtca cacaagaaga agtggacgag
     2941 ctagtagcac cgttattcga tatcgtacgc cgaatgtctt atgaggtgac agagatcgcc
     3001 ttagatcagc ctggggtaaa attgaatttt caatcagaaa aacaagaata gacagtaaaa
     3061 acatacctgc gatagattaa agctatcaca ggtatgtttt tttattacta attgtaagca
     3121 aaaagaatgt catttcgata aaaatcgtct aaaatataaa agtcactgaa aaaatttttt
     3181 tcaggaggat ggttgaagaa tcattctcgg gaacctttac ttccttcgat ggacttgtca
     3241 ttaataatat gaggaggaga aaaatgacaa cactcatcac agttttagtg gttctagttt
     3301 ttgcggcatt attatatgtg ttctatcgta tgcaaaagcg ccatacaaaa ttttcaacac
     3361 gcgtctttac agcattagga gttggtatcg tctttggcgg tctgcttcaa ttgatctttg
     3421 gtgtagacga caaagtcgtt actcaatcaa tggaatggat cgggattgta ggtcaaggat
     3481 atgtatcatt cttgcaaatg ctagttatgc ctttagtgtt tatttcgatc attggtgcat
     3541 tcacaaaaat gaaagtttct gaaaagctag ggaaaattag tgcgactgta ttgacttctt
     3601 tacttggaac aacagctatt gcagcattga tcggaattgg ttcaacaatg ctgttcggac
     3661 tccaaggagc taaattcaca caaggagctg cagaaacagc acgaatctct gaactggcaa
     3721 cacgtcagga aacagttgaa aacttatcga ttccacagca gattttatcc tttatcccta
     3781 aaaacgtttt tgctgattta gcaggtagtc gtgcaacaag tacgattgga gtcgttattt
     3841 tcgcggcatt tgtcggaatc gcttatctag gcgtgaaaag aaaagcgcca aaagaagcag
     3901 cattcttttc agatttagtc gacagtctgt ataaaatcat tatgcggatc gtgacgcttg
     3961 ttttgcgttt gacaccttat ggagtatttg ctttgatgac aaacgttttg gcaacaagtg
     4021 attttgcagc tatcgtcaat ttaggtaaat ttgtcgttgc ttcttacgta gcattgcttg
     4081 ctgttttgat tgtccactca ctgattttga ttggtgcaaa agtcaatcca atcaactatt
     4141 tcaaaaaagc ctttccggta ttgagctttg cgtttacttc acgttcaagt gctggagctt
     4201 tgccattgaa tatcgagact cagacaaaag ctttaggagt agatgatgca acagctaatt
     4261 tctcagcaag ttttggtttg tctattggac aaaacggctg tgctggcgtt tatccagcta
     4321 tgttggcaac gatcgtcgca ccagcagctg gagtgaatgt ctttagtttg gaatttattt
     4381 tgatgcttgt ggcaattgta acaatcagtt cttttggtgt agctggagtc ggtgggggcg
     4441 caacatttgc ttcactgatc gttttagggg caatgaactt gccagtagca atcgttgggt
     4501 tagttatctc tgttgaacca ttaatcgata tggcacgtac agcagtaaac gtcagcggta
     4561 gcatggtagc tggtatcgtg acaagtgcac gcatcaaaga tttagatcgc gatgtcatca
     4621 atgatgattc taaagtgatc gatgtgaatg catagtcaaa aagaggttgt gatagaagtg
     4681 gtcagcttta agaaataaga agaagtttcc taaaaatagt ttctcaaatt tttgtgaaat
     4741 ttcggcttat ttccgagaag ctacttctga aacaccgtga ataagaggtt gtgaaaaagc
     4801 tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg
     4861 gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc
     4921 ctgccgtttc actctgagtc acaaagagca atttcaaaaa atagcttctc atgtttgttg
     4981 aatattgtgt cttgtgaccg aggagttgtc aggagaacac cgtttaccag aggttgtgac
     5041 ttgtttttag tcacgacctc tttttttatt tattccatat aaatacacat tatttattcc
     5101 gaaaagtgaa agcgtaaata acaaaaacaa ccaacttatt tattattaaa gaaaagcgta
     5161 tttaagtagg acgattctgt tgagagcttt cgtagatttt cccattttcc tatggtaaac
     5221 tgacaaagtt gagaatgtta gaacag
//
LOCUS       DACDCV010000127         5001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDCV010000127 DACDCV010000000
VERSION     DACDCV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.129"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(4..1029)
                     /locus_tag="IV041_002587"
     CDS             complement(4..1029)
                     /locus_tag="IV041_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8383325.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="IV041_002588"
     CDS             complement(1274..2545)
                     /locus_tag="IV041_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8383326.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="IV041_002589"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="IV041_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="IV041_002590"
     CDS             3788..4261
                     /locus_tag="IV041_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8383327.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="IV041_002591"
     CDS             complement(4434..4988)
                     /locus_tag="IV041_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8383328.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1580 a          953 c          743 g         1725 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct t
//
LOCUS       DACDCV010000128         4977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDCV010000128 DACDCV010000000
VERSION     DACDCV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.130"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            870..1781
                     /locus_tag="IV041_002592"
     CDS             870..1781
                     /locus_tag="IV041_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383329.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2268..2534
                     /locus_tag="IV041_002593"
     CDS             2268..2534
                     /locus_tag="IV041_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383330.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2606..2947
                     /locus_tag="IV041_002594"
     CDS             2606..2947
                     /locus_tag="IV041_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383331.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            3002..3421
                     /gene="ssb"
                     /locus_tag="IV041_002595"
     CDS             3002..3421
                     /gene="ssb"
                     /locus_tag="IV041_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8383332.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3498..3794
                     /locus_tag="IV041_002596"
     CDS             3498..3794
                     /locus_tag="IV041_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383333.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3809..4240
                     /locus_tag="IV041_002597"
     CDS             3809..4240
                     /locus_tag="IV041_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383334.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4422..4727
                     /locus_tag="IV041_002598"
     CDS             4422..4727
                     /locus_tag="IV041_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383335.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1835 a          764 c         1036 g         1342 t
ORIGIN      
        1 tttttctttt gaaacaaatt cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa
       61 aaaccaattg aaaattgaag caaatttcac tcgaattgaa tcgattttga gctaaaatca
      121 atagaaaaaa taaacgtatt ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta
      181 gtaaggatta tttgagggtt atttagagat tatttaaagg ttatatgtaa taagtggggg
      241 gagggctgtc ggcgcttggt atgaaaggca ttctccgact tccaggtttt tgaccatttt
      301 ctctcaacgt ggaaaaaccg ccacttatga gagaaaatgg tcaaaaacct aagcgggaag
      361 tctccgaata acataccaag cgccgacagc caacagataa agaatacact actaaaaata
      421 gtagtagcat aaaatttaaa agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa
      481 aactaactcc cccctaaccc cctctttcag cctcttttga aagaggggga gaatcaaccc
      541 cacggccaac gccccagtgt tcttggggct gtccagaaca gaaacttgcg agagagtggg
      601 ggtaaaacaa gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagcc
      661 ggagtaaaac aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag
      721 ttggagtaaa acaagtgtag aggaaacttg aagacttgtt cgaacacaaa cttacaagag
      781 aggggagtaa agcagttgca gaggaatcag gaagatttgt tcgaacagaa acttgcaaga
      841 aagcattatt aaattgaagg tgatgaaata tgaagtattt attgcatggc tattttattg
      901 attattctac tcgcaatagt agtactttag cgattctatg tttgcttcat gaattgagag
      961 tggttactag aaaacagcta tacaaactat tgaattttga cagatcaatt agtaaacaag
     1021 gagtaacgaa tgctttagtg agattatatg aaaaacagtt aatcgatcga aaaaaatatg
     1081 gaacagaaat gtattattat ttgacgaaag agggacatca aagcataggt gggtattata
     1141 cgttgccgaa agttcctgaa tataatttga atcaccattt acaaattaac aattatttga
     1201 ttaaaggaat ggcattggca aaagaccacc cacatttaaa acggattatt tcagaaagac
     1261 gtaccgtcta tgaaacgaag gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc
     1321 cagattttat ttttcgtttt cgagacaaag aaaatcatga gatcaattgg tcctttgaaa
     1381 ttgagctaac gttgaaaaca cgtcgtcgct accgtgaagc gatttttccg aaatatattc
     1441 gtgagttgcg aacacgtcct gaatcacatt taatttatgt gacacctagt ggcgcaattc
     1501 aagaagaact tcatgcgttt aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga
     1561 gtcaacaaga attgtttgag cggttgcata ttttttcagc tttagagttt gaagagggat
     1621 taaaagcatt attagagaat gatccagcag tcaattggcg gacagtagaa cgagaaaaaa
     1681 tttatccgaa tggcgctgtt caaagtcgtt tgaatttatt tgaagaatca actgaaaatg
     1741 aggaaaaacc aaaagtgaag tttacgcctg aagtattgta aaagagggtg ttcgcttcgc
     1801 tctttttttg tgccagtttg ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt
     1861 ttaggaagga ctaacttgtt tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc
     1921 ctaatcgtat agcagtcatt cggcgagtat aacctaaaac ataacctgcc aattgccaag
     1981 ggtaaaatgc acgctgcgcg cccttggcaa tcgtcagaac atgttttcga gccggttatg
     2041 ccgaacgacc gctatacgaa aagggcctct aaacagaagt ccactttgat ccttgacaac
     2101 tgaaacccaa aatcaaacaa gcaggccttc gtctaaaaac gagccgaatg aagaccttgg
     2161 gggttgcacc cccaaacccc cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg
     2221 cgaaaaaatc aaataaaaca ttctattcaa gaaaggaaga aacaaccatg acaactaaaa
     2281 acgagcaact gaaagaacag gcaagaaaag agttgcaaga aagaggtttg attattgagg
     2341 ggagctttga aggagatttt gaaacctata ttggttgtta tgcaagaccg ataaacaaac
     2401 ctacagcctt agacccaatg aacgaacaag aagtaaaaga acaagaaaaa catgcagtga
     2461 atggttttcc gcaagatttc actgaatggt atgagtggga aattaacaac gggaagcttg
     2521 aaaacttctt ataaaagtca gatgacgtgc aaagagaatg attctctttg tacggtaaaa
     2581 atataaagaa agaaggaaaa agaaaatggc aaaaaacaca ccttatcaag taggagacat
     2641 tttttatagt agttggggct acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt
     2701 aacagaaaaa acagcatggt ttcagcctat tcaaaagaaa acggtcgagc attattcgtc
     2761 catgagtgga aaagttgttc caatgaaaga caagtttata gagtatcctt tagcaaaaac
     2821 taaagacagt attgtaaaaa aacgaattga tccagaaacc tatcctgata tgttatgtgg
     2881 ttatcgtagt tgggacacgt ttcatagata taaaaatcaa gctgtttatg aaagtagtta
     2941 ttattaaggg gatagtttcc cctttcaaaa ataataaata gaagaatgga gagaaaaaat
     3001 catgatgaat tcagtgaatt tagtaggacg gttaacaaaa gaagtggatt taaaatacac
     3061 accaaaaggc aatgcgacag gcacatttat tttagctgtt aatcgaaatt atacgaatgc
     3121 aaacggcgag cgtgaagcag attatatccg ctgtgtcatt tggcggaaag ctgcggaaac
     3181 tttagcaaaa tatactggaa aaggaagttt gattggcatt aatggacgtc ttcaaacgag
     3241 gagctatcag aatcagcaag gacagaccat gtatgttacg gaagttctag tgacagattt
     3301 ctatttgtta gagagtaaag aaatcaatga gcaacggtca aaatcaatgg cgaataatca
     3361 aaataattct tcaattccag gaaatgagat tcaaatttca gatagttatt tgccctttta
     3421 agtgaaaaat aattttgttc tatacctgat ttcttcaggt atagaacaaa aaataaatag
     3481 agatgaggcg attttttatg aaaatcattg acagttacga tcaacgaacc atgcaatatt
     3541 tgggctcgtt tgattataca gatgaaaata ttattaatta tgttgcaagt ttacctcctt
     3601 atcaaagcgt tcgcttggta gattttgcaa gtgatgaatt ggtattaaca acgatgggca
     3661 actttttaga ttatgtcgga gacaaaaaat ggttggaaaa aattttgtct aaattgattg
     3721 cgaaacaaac aggagaagaa cccataaatg cgattcaatt gttcaatcga tatgaacaag
     3781 aaaacaatga ttaaagagga gagaaaaaat gaaaagatta aaacgaagta tttttattgt
     3841 gctagcattg attgattttg ttgggacagg ttgtgaagca attgacgttc aaaaagctcc
     3901 cttatcggaa ataaaacaag caataacaaa acaagatgaa gaacaaccgc taaatacaca
     3961 agtagaaata gtgaatcaaa ataaaccgag tttttctgaa gaagattttt cgttagaaaa
     4021 tggaaccagg caattttttt cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc
     4081 acttttgcat aaaagcagga tgccaacaga agaacgagac cgattataca ttaagccaac
     4141 tggctggaaa caaaagaaaa tgaagaatgg cgattatctt tataatcgtt gtcatttgat
     4201 tggctatcaa cgtgcgacaa gtttccccat aagtatgtaa tacgaaacgg gaggattaat
     4261 cttaagatta atcacaacta acctatttga cagttggaat gaaagctatc atgtctgctg
     4321 aatcaactgg tctgatactc ctacatgcaa taaagtctac taggaagtta ttgtatgaag
     4381 ctaggtaaag gcggcagaac aatttctaag tgtggaatca tatgaagttg gatatgccgg
     4441 gacgctataa attactcagg gtgagactgt ttttaattaa actgacgaac ttgcgaatgt
     4501 acgggtctat aaccgactta tattcgaaag atataagatt gcgaatgcaa agaacattat
     4561 cgaaaagtaa gaatgttggt catgaaattc gagatggaag actagcggtg tatctgtcaa
     4621 gagttggtaa aacatgtttg attgattcag atgagagtgg gaatgcgaca aaacgtttta
     4681 cagaagaaat agagcaacta gcagaactga atatacgata tttttaaata ctttagcgga
     4741 gaataaaaaa atcctctaca gaaaactacg gagggcaaca tgtgctattg tttcaacaat
     4801 acatttttaa tttttaccat aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat
     4861 taaaaatgaa aaaatggaag cggttactta ttcttttgaa tctaagataa aaattcaaaa
     4921 gaataacata acccaaatat gattatactt gagattctaa atatttatcg aaaagga
//
LOCUS       DACDCV010000129         4895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDCV010000129 DACDCV010000000
VERSION     DACDCV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.131"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..2325)
                     /locus_tag="IV041_002599"
     CDS             complement(91..2325)
                     /locus_tag="IV041_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8383336.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2804..3070
                     /locus_tag="IV041_002600"
     CDS             2804..3070
                     /locus_tag="IV041_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8383337.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3070..4797
                     /gene="ptsP"
                     /locus_tag="IV041_002601"
     CDS             3070..4797
                     /gene="ptsP"
                     /locus_tag="IV041_002601"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8383338.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1503 a          917 c          990 g         1485 t
ORIGIN      
        1 tttataaaat gaacagtatt ttgacttttc tgaaaaaaga atgagacggc atttctcaaa
       61 gaaataccgt ctcacgctat aaaactggat ttattcagcc gtttcttcgg ctgattcttt
      121 agccaaacgt tctggtttag aagtaacgat gatttttcca tctttatcca gttttgcttt
      181 caactcatgg atagaagggt gatcaagata aaattcagcg ataccatctt cgatttgttc
      241 ttggatcgtg cgtcgaagag gacgagcacc catagatgga tcatagccta agtcaactaa
      301 tttttctttg acattcgtag gaacttcgat gtgaagttgc tgtgctgcca gcatttggtt
      361 gacatcgtct agcattagtg tgacgattgt catcaagttt tcttttgata atgcactgaa
      421 ttcgatgatc ccgtcaaaac ggttcagaaa ttctggtgta aagtagttgt ttaattggtt
      481 caatacagaa cgagtaacac cttcgcgtgc cgcaccaaaa ccaacgtttg cttctacttt
      541 accagttccg gcattacttg tcataatgat gatcgtatct ttaaagctga ccgttcttcc
      601 ttgtgcatct gtcaatcgac catcatctaa gatttgcaag aacatatgca aaacatctgg
      661 atgagctttt tctacttcat ctaacaagac aagactgtaa ggatttctcc gtactttttc
      721 tgttaattga ccagcttctt cgtatccgac ataacctgga ggagaaccaa tcaatttaga
      781 gacactatgt ttttccatat attcagacat atcgaaacga atcattgaat cttgtgaacc
      841 gaatagttcg taggcaagtt gtttcgcaag ctctgtttta cctacaccag ttggtccaac
      901 aaataagaag gagccgattg gacgattctg tttgtttaat ccaacacggt ttcggcgaat
      961 cgctttcgct actcgatcta cagcgttatc ttgtccgatg acatgagctt tcaagtcatc
     1021 tgctaagttt ttcagttgtg tctgttcttt ttcttttaat tcacccactg gaatacctgt
     1081 ccgttgttca acgatttttt ccatatcttt ttcggtaatc acaggtgttt cctcttcagt
     1141 gagctgacgt tctttcattt tttgaagttt attgatctga tcgcgataat aagctgcttt
     1201 ttcgaaatct tcttctcgag aagctaaaac tttttgttct tctgcttctt gcagtttttt
     1261 ctcgattgtt ttaggatcaa cgatttggat cgtcaagttt ttcttagaac ctgtttcatc
     1321 tagcaaatcg atggctttat ccggcaagaa gcgatcttgg acgtaacgat tggataaagt
     1381 tgcggctgct tcgatggcct catcagtata ttttacatga tgatagtctt cataacgttt
     1441 ttgcaaacct ttcaaaatac tgatcgtttc ttcgacagtt ggttcatcta cccgtacagg
     1501 ttgcatacga cgttctaaag cagcgtcttt ttcgatgatg cggtactcat ttaaggtagt
     1561 agcaccaacc atttgcaatt ctcctcgagc taaagctggt tttaaaatat taccagcatc
     1621 catatttcca tcaccagcag aaccagcacc gacgatttcg tgaacttcat ctatgaatag
     1681 gatcacattt tctgcttgac ggatctcttc aatcaatttt tgcatacgtt cttcaaattg
     1741 tcctcgtata ccagtacctt ggactaaaga tacgacatct aaacgaatga cttctttgtc
     1801 cattaatttt tgcggtacat caccatctac gattttttgt gctaaacctt caacaactgc
     1861 tgttttacca acacctggtt ctccaatcaa gacaggattg ttcttggttc ggcgatttaa
     1921 aatttcaatg actcgtttga tttcgtcatc gcgtccgatc acaggatcga tatcaccttg
     1981 tcgtgcttgt tctgtgatat tgattccgta ttctcccaat aaaccttctg tattgccttg
     2041 aggtggctga ccaccattga agttgttgtt tccgccaaat tgtgttggtg gtgtttgctc
     2101 ataatttcct tgctgttgca tttggcgaga catggaacgg aaaagatcgt ccaaactgcc
     2161 aaatccaaat ggatcatttt gggccatatt agtcagacct ccattttgtt gatttttgat
     2221 tttttgataa cagctctgac agtaatcgag ttgtgtccgc tgaccattga cagtggcata
     2281 caagtgaatc gttgcctcat tcttgccgca gttttgacaa agcatgaatc attcacgtcc
     2341 tttcacaaaa atgggtacat ccatatttta catctcgttt ttcatgaggt aaaagaataa
     2401 cccctagttt tttaagagat ttatgaggta gaaaaggtga aatctctatt gaccattcct
     2461 gacctttgac tcttattata ctaaaaataa aaagggaagc aagtaattcg tttcatatga
     2521 aaacaaagag ggtcaaagga aagaatgata aacgtcagga gaagagaaag cgacaagaaa
     2581 aaggaaataa acacgtttgt tataaaatgt gaagaaaagg atgaaaaaga atccggaata
     2641 atttgaatac ttttatctga aattgaatga aaactgttgt atctaacgaa aaaaaatgtt
     2701 aatctttaca gatgaaaggg tggcatttgt taggattctc tgaattacta aaaaagaaaa
     2761 ccctcacata aaaacaacac tcacttaaag gagaccgatt aatatggaaa agaaagaatt
     2821 tcacgtagta gcagaaactg ggatccacgc tcgtccagct acactattag tacaaacagc
     2881 aagcaaattt aactctgatg taaacttaga gtataaaggt aaatcagtaa acttgaaatc
     2941 aatcatgggc gttatgtctt taggtgtagg tcaaggttct gatgttacta ttactgcaga
     3001 aggtgctgac gaagctgatg caatggcagc aatcgttgaa acaatgaaga aagaaggctt
     3061 atctgaataa tggttgaaat gctaaaaggg attgccgcta gtgacggtgt agccgttgca
     3121 aaagcttacc tgctagttca accggattta acattcagta aggcaacagt agaagatact
     3181 gcggctgaag aagctcgttt ggatgctgca ttagctaaat ctactgaaga attgcagcaa
     3241 attcgtgaaa aagcagcgca aagcttaggt gaagcagaag cgcaagtatt tgatgcccat
     3301 ttgatggttc tttcagatcc agaaatggta ggtcaaatca agcaaaatat taaagacaac
     3361 agcgtaaatg ctgaatcagc gctaaaagaa gtaacggata tgtatatcgg tatgttcgaa
     3421 gcaatggaag ataatgctta tatgcaagag cgtgctgcag atattcgtga cgttgctaaa
     3481 cgtattttag cgcacttatt aggcgttact ttaccaaatc catcaatgat caacgaagaa
     3541 gtggtcgttg ttgcacatga cttgactcca agtgatacag cacaattaga tcgcaatttt
     3601 gtaaaagcgt ttgtaacaga cattggtgga cgtacttcac attctgctat catggcacgt
     3661 tctttagaaa ttccagcaat cgttgggaca aaagaaatta ctgctaaagt caaagaaggc
     3721 gatattttgg ctgtcaacgg gatcgaaggc gatgtaatca tcgatcctac agatgaacaa
     3781 aaagctgaat ttgaaaaagc aggtgcagat tacgcagcac aaaaagcaga atgggaaaaa
     3841 ttaaaaaatg cagaaactgt cacagctgac ggcaagcatt ttgaattagc tgcaaacatc
     3901 ggaacaccaa aagatctagt tggtgtccat aataatggtg gagaagcagt tggtctgtac
     3961 cgtactgaat tcctgtatat ggattctcca gacttcccaa cagaagatga ccaatatgaa
     4021 gcttacaaag ctgtattaga aggtatggaa ggaaaacctg tagttgttcg tacaatggac
     4081 atcggtggag ataaagaact accatatctg caattacctc atgaaatgaa tccattctta
     4141 ggatatcgtg cattgcgtat cagtttatct gaacaaggcg acgaaatgtt ccgtacacaa
     4201 atgcgtgcat tgcttcgtgc atctgttcat ggtaatttac gtatcatgtt ccctatggta
     4261 gctacactca aagaattccg cgcagcaaaa gcgatctttg aagaagaaaa acaaaaattg
     4321 ataagtgaag gcaaggaagt atctgatacg atccaagtcg gcatcatgat cgagattcca
     4381 gcagcagcag tcttagcaga caagtttgca aaagaagtag atttcttctc tgtaggtacg
     4441 aacgatttga ttcaatacac aatggcagca gaccgtatga acgaacgcgt ttcttacttg
     4501 taccaaccat ataacccatc aattttacgt ttgatcaaaa atgtaattga tgcagcacac
     4561 gctgaaggaa aatgggcagg tatgtgtgga gaaatggctg gagatcaaac agctgttcca
     4621 ttgctagtag gtatgggtct agatgagttc tcaatgagtg cgacatctat tcttaaaacg
     4681 cgtagtttaa tgaaacgctt ggacacagcg aaaatggctg aacttgctga tcgtgcattg
     4741 aaagaatgcg atatgatgga agaagtcgtt gaacttgttc atgaatatgt aaaataaaca
     4801 acgtatcaaa agctggtgtg gcttgtaaaa gagctacgcc agtttttttg ttctttttta
     4861 agaaaacgtt tttgtcagta agaatagaca aaaat
//
LOCUS       DACDCV010000130         4889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDCV010000130 DACDCV010000000
VERSION     DACDCV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.132"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..653
                     /locus_tag="IV041_002602"
     CDS             <1..653
                     /locus_tag="IV041_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8383339.1"
                     /translation="VAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQIDKV
                     YVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTTAPF
                     ISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLYEET
                     SGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQGS"
     gene            672..2255
                     /locus_tag="IV041_002603"
     CDS             672..2255
                     /locus_tag="IV041_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8383340.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2390..2968
                     /locus_tag="IV041_002604"
     CDS             2390..2968
                     /locus_tag="IV041_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383341.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            2914..3432
                     /locus_tag="IV041_002605"
     CDS             2914..3432
                     /locus_tag="IV041_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383342.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3440..4480
                     /locus_tag="IV041_002606"
     CDS             3440..4480
                     /locus_tag="IV041_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8383343.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            4729..>4889
                     /locus_tag="IV041_002607"
     CDS             4729..>4889
                     /locus_tag="IV041_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383344.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1797 a          565 c          957 g         1570 t
ORIGIN      
        1 tagtcgctat agtaccaatg aagttaaata acaaaagatt gccacaaaaa aacacgaaag
       61 cttttaccaa tggaaaacca ttgtgttatt atatactttc aacactttta aaagtaaaac
      121 agattgataa agtgtatgtc tattgtagta acccagatat taaagaattt ataccagaag
      181 gtgtaacatt tttaaagaga tctagttcct tagatcagga tacaacgagt attacacagg
      241 tattagccgg tttcacaaaa gaagtagagg ctgatgtata tgtaatgaca cacacaacag
      301 caccatttat tagcgaaaaa tctattgaaa aaggactaga tgccgtgctg acaggagaat
      361 atgattctgc atttgctgca aaaaaattgc aagatttctt atggaaagac ggtgaaccat
      421 ttaattatga attggacaat attcctagaa cacaggattt gccgccttta tacgaagaaa
      481 ctagtggatt ttatatttat aatcgcaatg tgatgaccga gttgcataga agaattggga
      541 aaaaaccttg catagttgaa gtaggtgaga ttgagtcagt tgatattgat gaagccgagg
      601 attttatgat tgctgatgcg atttataatc atcttatttt acaaggtagt taagacaggg
      661 ggaaggttag gatgaacaat gtacagattt tagattgcac acttagagat gggggctatt
      721 gtaacaattg ggaatttgga tttgataata caaaaaaaat tatttctgga cttatagaat
      781 caggaattga gattatagaa tgtgggtttc tcacaaataa aacagtatac aataaaaatg
      841 ttacaaaata tacgacctta gaagaattag cacagataat tcctgaaaaa agagaaggaa
      901 aattatttgt agcaatgatg aactatggcg aatataatat agaggatctt ccagattatg
      961 atggttcatc tattgatgga attaggattg cgtttcataa aaaaaattgc aatgaagcgt
     1021 tgaaattatg tcgtgagata aagaaaaaag gctacttagt ttttgttcaa gctatggtat
     1081 ctctcaatta tacagatgaa gagtttttag cagtaataaa aaatgtgaat cagtttaaac
     1141 catacgcttt ttatattgtt gatagttttg gaatgatgaa aggaaaagat ttaactcgtc
     1201 ttttttacat gattgagcat aatctagaaa aagatataca gataggtttt cactcacata
     1261 ataacatgca gcttgcatat tctaatgcac aaaaacttat aaccgtacaa acaaatcgaa
     1321 gcctgattat tgattcttcg gtgtacggaa tgggaagagg agcaggtaat cttaacacag
     1381 aactatttgt agaatatttg aatgataatg caggaaaaaa atatcaatta aaaccattat
     1441 tatatataat tgatgaaata ctaaatagtt tctatcagaa gagttattgg ggatattcat
     1501 taccaaacta tatttcagca gttcataatg ctcatcctaa ttatgcaagc tatctgtcat
     1561 ataagaatac tttaacaatt gaagcaatga atgaaatatt cgaaatgatg gatgaagaaa
     1621 aaaaggtatc ttatgataaa caatatatag aagatttata ctacaaatat atggaaactg
     1681 ggaaaataca ggaggaatat atggaagaac tgagaaaatt tctctcagac aaaaaaattc
     1741 tactgattgc accaggaaaa agttctgtag atgaggtaga aagaataaaa gagtttgtta
     1801 ccaaagaaga agtcgtaata attagtgtaa attttgaata ttctcatata gatatagatt
     1861 acgtattttt aagtaattta agacgttttc atgaattacc gaaagaaagc agaactaaat
     1921 gtattgttac atcaaatata caagcatcgg atgtatattt acggacgagt tataaaaagt
     1981 tgttatctga aagggaagtt gtaagagaca atgcaggatt aatggcaatt agatttttag
     2041 ccgatatgaa tgtggatgaa atattcttag ctggatttga tggatattca cataatgaag
     2101 atgagaatta tggtgagcaa tccatggaaa ttataacaaa atttgcatta ttagatgcaa
     2161 tgaacgtgca aatgagagat gaattgtcag aattagctaa aaaagtaaag gtgacatttt
     2221 taacggatcc acgttgggtt cgaattgata agtagtagta ataatgtttt taaaattttg
     2281 ataaagagat agtgagagaa taaaaatggg taaagtctta aataaaaaag aagaaaaata
     2341 tttaagggac gatggggcga gatgaaactc taattttgaa cttttaagaa tcatttctat
     2401 attgtgtatt gttgcttacc atgctaattt gcgtttaaat tataatgcag acggaattat
     2461 agtacatggt tcattaactg ctaaccagat tttcttgaat tatattggta tttggggtca
     2521 gctaggagtt aattgtttta tatttatttc ggcatatttt ttaagtgaga aaagaaacat
     2581 tcactttgaa aatgttttgg gaattgtatt agaatgtatt ttttatagtg tattagttta
     2641 tggaattctt tgtgcatgtg gaatagaaac attttcattg aacagctttg taaaagaaat
     2701 attatcattt gctctaggta gatattggtt tattacatcc tatttagcat tgtatattct
     2761 aagtccgtat ttaaatatca tgcttaacaa tctggaaaaa cttcagttgc aaaaaataag
     2821 tatgttattg tttataggtg tatgctgtta tcgtatgtta tttaaaacat ctttgtttgg
     2881 ggattgtgct gttttaattg cactatatgt catatgttat acactgaaaa aaacgataat
     2941 aatatttttg agagacacag aatcatagga acaattgtat gtataacagt tataccactt
     3001 ctcatatcca taggaatact tttcaaattt ataccaaatg caatggttaa ccagatggtt
     3061 catattggtt ctccattgat ggttatagca agtatatttt tattttatgt atttaaaaat
     3121 ttttcatttt acagtaagta tataaataaa cttgcgagaa ccagtttagg ggtatatatt
     3181 ttacatgaaa atcattattt cattgatggt atgaagtcta gaggcttaat atgggatggg
     3241 atatttggat taggcaaaag gtttttagat agggattttt ggatttggtt tataattgca
     3301 gttgtagttg tgtgcataat ttgcatggct atcgatgttg taagagaaaa atttgaaaga
     3361 cagattttta aaattaagag tgtaaagaac ctgtgtaaac ttatgaatca tgttataaat
     3421 atatacccat aattaataaa tgaggagaaa agaagtgaaa gttatatcat tcggagaatt
     3481 attattgaga cttgcagcac ccggttattc aaagttattt caaagagata gtttggagac
     3541 tacattctgc ggaggggaag ctaatgtagc ggtatcctta gcaatatttg gatatcaatc
     3601 agaatttata acaaagcttc cggattcaga tgttggatat gcggcagcta atgctttgcg
     3661 ttattttgga gtaaatgttg ataatattat atttggagag ggacgcatgg gactgtatta
     3721 tttagaaaaa ggagcagctc agagaccatc taaagtaatt tatgacagaa aattttctgc
     3781 aatttcgtta gctgatagtg atgaatttaa gtggaaggag atcctaaacg atgcaaattg
     3841 gtttcattgg acagggataa atccagcatt gtctgagaat ctctttgaga cgtccttaga
     3901 tgcatgtcag gctgccaaaa aaagaggtat aacaatttct tgtgatttaa attatcgatc
     3961 caagctttgg acatcagagg aagcaaaaga aaagatggaa cagatactac cattggttga
     4021 tatttgtatt tgtaatgaag aagatgcaga aaaagtgcta ggaattaaag ctgagcatac
     4081 aaatattaat agtggagaat taaataaaga tggctatgtg caggtggcta gggagattca
     4141 caagaagttt ggatgtaggt atgtagctac aactctgcgt agaagttatt cggcaagtag
     4201 aaatggttgg agtgcaatgt tatacgaagc agaaaaagat aaagcgtatt tttccacaga
     4261 atacaacata caaattgttg atcgcgttgg tggtggagac agcttttctg ctggtataat
     4321 atatgcgtta tgtgaaaata tgtccggaca aaaggctatt gaatttgcga ctgcatctag
     4381 ttgtttaaaa catactatag aaggtgattt taatcgaact acaattgagg atgtatataa
     4441 tttaatgaat agcggtggaa atggtcgcgt gttgcgttag tcaacatata aagataaatc
     4501 acagtaattt ttatggtgat tatctgtctt agtagtgatt agaataacgt gaaaataaat
     4561 tgttgagcgt ttatactgtt ttgaaaaata aatttaacat attgaaaaat acaaatatat
     4621 acgtatatta taaagcgccg attgtaaaat taagctagac aactaaaaca gtcatttgtg
     4681 ctacactcaa aatagttccg accaaagaat tataaggagt gagtgcaaat aacctatacc
     4741 tatcttacaa cggatgaact tgtaataata gagtcttatt tgaaaattaa ccaatctgtt
     4801 gctaaaactg cgcattgctt gaatcgttca agtcaaacga tccataaagt atatctgttc
     4861 ttcaagcaaa gaaaatcagc cttagaata
//
LOCUS       DACDCV010000131         4882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDCV010000131 DACDCV010000000
VERSION     DACDCV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.133"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IV041_002608"
     CDS             complement(406..780)
                     /locus_tag="IV041_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8383345.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IV041_002609"
     CDS             complement(803..2203)
                     /locus_tag="IV041_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8383346.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IV041_002610"
     CDS             complement(2207..2617)
                     /locus_tag="IV041_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8383347.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IV041_002611"
     CDS             complement(2635..3126)
                     /locus_tag="IV041_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383348.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IV041_002612"
     CDS             complement(3136..3927)
                     /locus_tag="IV041_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8383349.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IV041_002613"
     CDS             complement(3920..4693)
                     /locus_tag="IV041_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8383350.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1552 a          995 c          647 g         1688 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga tt
//
LOCUS       DACDCV010000132         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDCV010000132 DACDCV010000000
VERSION     DACDCV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.134"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IV041_002614"
     CDS             564..989
                     /locus_tag="IV041_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383351.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IV041_002615"
     CDS             2141..3118
                     /locus_tag="IV041_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8383352.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IV041_002616"
     CDS             3128..3625
                     /locus_tag="IV041_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383353.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IV041_002617"
     CDS             4001..4282
                     /locus_tag="IV041_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383354.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IV041_002618"
     CDS             4284..4700
                     /locus_tag="IV041_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383355.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1756 a          817 c          785 g         1430 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 tcagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDCV010000133         4703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDCV010000133 DACDCV010000000
VERSION     DACDCV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.135"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..639
                     /locus_tag="IV041_002619"
     CDS             193..639
                     /locus_tag="IV041_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383356.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            641..778
                     /locus_tag="IV041_002620"
     CDS             641..778
                     /locus_tag="IV041_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8383357.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            815..1108
                     /locus_tag="IV041_002621"
     CDS             815..1108
                     /locus_tag="IV041_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383358.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1105..1329
                     /locus_tag="IV041_002622"
     CDS             1105..1329
                     /locus_tag="IV041_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8383359.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1326..2345
                     /locus_tag="IV041_002623"
     CDS             1326..2345
                     /locus_tag="IV041_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8383360.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2534..2607
                     /locus_tag="IV041_002624"
     tRNA            2534..2607
                     /locus_tag="IV041_002624"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2568..2570,aa:Ile,seq:cat)
     gene            complement(2972..3178)
                     /locus_tag="IV041_002625"
                     /pseudo
     CDS             complement(2972..3178)
                     /locus_tag="IV041_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3522..3929
                     /locus_tag="IV041_002626"
     CDS             3522..3929
                     /locus_tag="IV041_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383361.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            3943..4344
                     /locus_tag="IV041_002627"
     CDS             3943..4344
                     /locus_tag="IV041_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383362.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            4346..>4703
                     /locus_tag="IV041_002628"
     CDS             4346..>4703
                     /locus_tag="IV041_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383363.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT         1659 a          737 c          894 g         1413 t
ORIGIN      
        1 gcttattcat ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta
       61 caaatcaatc aagccttacg gattcgagca aatcattgtg aatgacgaag aacatttacc
      121 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
      181 ggagggaaat aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga
      241 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg
      301 gttcgttcag atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac
      361 caatggatta ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg
      421 tacgaggctc ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta
      481 agcgatgtag cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca
      541 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
      601 aaagagcaac taaatccagc ttcaaaagga ggtgagtagt atgtttagtt ttagcgatgt
      661 gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact
      721 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
      781 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
      841 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
      901 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
      961 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1021 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1081 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1141 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     1201 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     1261 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     1321 aacaaatgaa aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat
     1381 tgccaattaa cggatttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag
     1441 gtagctcaca agtagcaaat aatcagtata ttttactgca tgaaacggct aatgaaacag
     1501 caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt
     1561 atattgtggg agacggcgga attgtttacc aagtcggaca acctggttat gtacagtacg
     1621 gtgctggttc atatgctaat gccaatagtc ctgtgcagat tgagttacaa cacacacatg
     1681 ataaagcaac ttttgaaaaa aactataaag catacgttga attggctaga gattcagcaa
     1741 tgaaatatgg tattccatta acattagaca cgccttataa ccaaccagga atcaaatcac
     1801 atttatgggt aactcaaaac atttggggcg atcacactga tccgtatgga tacttatcag
     1861 aaatgggtgt aagtaaggaa aaactggctt acgatttagc tcatggattt acagacgaaa
     1921 acccaacgac ttctgagaac aagcctgtca ttgatccaac ccgagcaggt gcagcaaatc
     1981 caactttatc agatggaacg aaccattctc acattgatca gtttggagaa atcgaaaatg
     2041 cgaacttaca cgtcgctgga tggcacatcg ctaactataa gtacgagtat attttcatta
     2101 tggactacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagaccgg
     2161 atgtaaatca agcttataat acttctggaa atgttggcta tcatgtatct tttaacatgc
     2221 gtaacttccc taataagaaa gtctatgtca tgatgcgggc aacgaatgat ccagagggaa
     2281 acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac
     2341 gataaaaata gctcctcgtt gaggagcatt acataactat attgacaact ataaaaatca
     2401 ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccataatag tcacactcca
     2461 agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt atggctttat
     2521 gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg
     2581 tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga
     2641 attttttgaa gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc
     2701 ttttttatgt atccttttat ggattaatga aaggatgttt cacatagtta tatttctgta
     2761 tatttgaaaa gttttacttt gatttttaaa tagaaagaca tttgggttat attgtgagat
     2821 aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc
     2881 tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga
     2941 gaaaaaaggc ttataattgg agtggtagtt attctataac atcaagttta aattttgtag
     3001 tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa atccttaccc
     3061 aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg tagccgcctt
     3121 cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat ttccgcaaaa
     3181 aaagcacccc ttttaattag atttctagtc taacttttgg ggtgcacatc atttttgagg
     3241 ggaagtacat atagttgttg atatagttgc taatattgtt agctaatttg ttagtaaaac
     3301 tgtatacgaa tttagatagt aaaaaaacag tttgttcctt atatagaggc acttttacta
     3361 ctgattaaat gtcagatatg cttacaaata tacttataaa tatgcttaca aaattagcta
     3421 atatttccgt tgacaaagct ttgttactat tgttaaacta aattgactag caaaagtgaa
     3481 acataaattt attaaataaa tgtaaaaatt aggagaaatc aatgtcttat agagtacaat
     3541 taataatttc agaagatgta gaaagtcagc aatttggcac taatgttatt aataaagtaa
     3601 ttaatccagc tctttctatt aatgcgcctt taattcctac tgctttatca ttttctgtaa
     3661 cagctgtggt gtctgagata gaggatacag aaaagataaa aatagtagaa attgaagttt
     3721 taaacaagaa tgaaaaacaa attttttcta cgggtgaagt atcggtaagc ttgcctcctc
     3781 aagttaacga tataaacttt aatataaacg ccagaaatgt cttggttgaa gaagcaggag
     3841 agcattatgc tgttgttaaa tttaatggaa ctgagattgg aaggcaaatc tttgatatca
     3901 aggtcaacaa accagtggaa aaaaattaag gagacgatac ggatgcttga tatagtacca
     3961 tcaaatacac atcggagtgg tagcttaata aattttgctg cgatttttgc ttgcgcatcg
     4021 gtaattctag catctccagt tgttgtggat gcaagttcta ctccgaaaac taaaaatgat
     4081 aatcaacttg taattactac aaaaaatgag attaacacaa tttcaagtaa tgatggcaaa
     4141 atttttgatg tgataaatac ggttttaaaa agaaaagctg aaatcaatat aaaatatgat
     4201 gaagatttaa acttgtattt ctttgttatc aaaacaacat ctgaattgtt ttcctcagat
     4261 tacgatgttt tggatactct cgataatgta ttatctgact ataaatatat ggggaaatct
     4321 gtagtagcaa ctttggggga ataaaatgtt tgactttaat gaatatttat ctatttccaa
     4381 acaaattgaa tcagatgata aatataattc taaagaatct tatagaagaa ctgctgtttc
     4441 aagagcatac tacagtgctt ttaaaaaatc tgatgagtat ttgaaagaaa attatgatat
     4501 tatttataat ggaagtagcg gaaaaggaag ccaccaaacc gtttggaatt tgttttcaac
     4561 agttaaggaa ttgaatacct taggaataca taatagtggg tatagaatgt tagagaaaag
     4621 aaaatgtgct gactatattc ctaatgaaac cattacgaaa acagatatgg cgctgatgaa
     4681 tcgagaagca gaaaaaataa taa
//
LOCUS       DACDCV010000134         4522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDCV010000134 DACDCV010000000
VERSION     DACDCV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.136"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IV041_002629"
                     /pseudo
     CDS             106..357
                     /locus_tag="IV041_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IV041_002630"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IV041_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8383364.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IV041_002631"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IV041_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IV041_002632"
     CDS             1786..1869
                     /locus_tag="IV041_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8383365.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV041_002633"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV041_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8383366.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IV041_002634"
     CDS             complement(2994..3857)
                     /locus_tag="IV041_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8383367.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IV041_002635"
     CDS             complement(3859..4131)
                     /locus_tag="IV041_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8383368.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IV041_002636"
     CDS             complement(4148..4363)
                     /locus_tag="IV041_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8383369.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1395 a          761 c          837 g         1529 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat gg
//
LOCUS       DACDCV010000135         4478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDCV010000135 DACDCV010000000
VERSION     DACDCV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.137"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            326..1057
                     /locus_tag="IV041_002637"
     CDS             326..1057
                     /locus_tag="IV041_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8383370.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1202..3229
                     /locus_tag="IV041_002638"
     CDS             1202..3229
                     /locus_tag="IV041_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8383371.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3337..4434
                     /locus_tag="IV041_002639"
     CDS             3337..4434
                     /locus_tag="IV041_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8383372.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1575 a          719 c          923 g         1261 t
ORIGIN      
        1 ccaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
       61 cagtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
      121 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
      181 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
      241 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
      301 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
      361 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
      421 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
      481 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
      541 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
      601 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
      661 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
      721 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
      781 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
      841 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
      901 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
      961 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
     1021 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
     1081 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
     1141 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
     1201 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
     1261 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
     1321 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
     1381 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
     1441 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
     1501 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
     1561 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
     1621 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
     1681 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
     1741 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
     1801 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
     1861 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
     1921 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
     1981 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
     2041 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc
     2101 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
     2161 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
     2221 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
     2281 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
     2341 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
     2401 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
     2461 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
     2521 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
     2581 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
     2641 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
     2701 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
     2761 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
     2821 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
     2881 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
     2941 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
     3001 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
     3061 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
     3121 ttctacagct catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac
     3181 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
     3241 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
     3301 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
     3361 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
     3421 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
     3481 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
     3541 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
     3601 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
     3661 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
     3721 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
     3781 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
     3841 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
     3901 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
     3961 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
     4021 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
     4081 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
     4141 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
     4201 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
     4261 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
     4321 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
     4381 ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa
     4441 gataacaaat agtaaatttt tattattgta ggatggtt
//
LOCUS       DACDCV010000136         4452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDCV010000136 DACDCV010000000
VERSION     DACDCV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.138"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..1663)
                     /locus_tag="IV041_002640"
     CDS             complement(20..1663)
                     /locus_tag="IV041_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8383373.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1660..2019)
                     /locus_tag="IV041_002641"
     CDS             complement(1660..2019)
                     /locus_tag="IV041_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383374.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2180..2440)
                     /locus_tag="IV041_002642"
     CDS             complement(2180..2440)
                     /locus_tag="IV041_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383375.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2431..2742)
                     /locus_tag="IV041_002643"
     CDS             complement(2431..2742)
                     /locus_tag="IV041_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8383376.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2743..3315)
                     /locus_tag="IV041_002644"
     CDS             complement(2743..3315)
                     /locus_tag="IV041_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383377.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3648..3908)
                     /locus_tag="IV041_002645"
     CDS             complement(3648..3908)
                     /locus_tag="IV041_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383378.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3898..4086)
                     /locus_tag="IV041_002646"
     CDS             complement(3898..4086)
                     /locus_tag="IV041_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383379.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1304 a          951 c          634 g         1563 t
ORIGIN      
        1 tttcaataag gagtatacct taaaattcaa tctcatccaa catatcaaat gcttcctcat
       61 aatccatatc agttatttct tctcgtttat ataaagctgc caaaaaagca tgaaacccat
      121 ctgttttacg ccttactggc tcttttttca aaaatgtttt attccctaat ttgtcttctt
      181 tgataaacga attatttgta taccaacgca tcataggatc atcaccaaaa ataaaccgtt
      241 catttgcaaa accatcttca ataataggag caacttttga ctgcacacct cttatatttc
      301 tgataaactc aaaattatat gattctttct ctagaagagg tttcagcaaa tccatacgaa
      361 atccgtcggc acaaacgatt tctatttgat ggatttttct tttttcattt aaccaatcaa
      421 ttaaatgacg aggatcaatt gatggctcat caacgattgt cactaaacct tttttctccc
      481 aatccttaat aggcgctttc agtttgaatg tatctagaaa ctttttccta gcaaaactat
      541 gttgctgcca gataaattta tcatcaattt taaaaagcaa acccactgtt gcaaaatcac
      601 gaatacttgc ataatcaaat ccagccacac aagaacgacc attcaaattt ggcaattctt
      661 gtttagtagc cattaatttt tcatgagtcg taatatctga ttctgcatca ccttctgtaa
      721 aattcattcg tttaacaaca aattcacgcc tacctgatgg ctcatcttct agttttttat
      781 attctttcat cactgtagaa aacagacgtt ttgaatatgc tgaatcttct tcgaacattg
      841 gattagcctt tgaccacatt ttaggattat ccatttcatc aatgttatcc agtttacaaa
      901 taaagggaaa caagcgatca ttcttgttct ctccactaaa aatcttttgt gctcgttcca
      961 acattttgtc ataaaaacct tctctaacat gaccatttgt accattataa aaagttcgag
     1021 gatgttttat tttccctagc ccactacgct gaatatcaac taggtctgaa gattcaaact
     1081 gatggatttc atcaaattct aagcagccat ctcttgcact atccatagtc ttaggattgt
     1141 ttgtcctata actaaaaatc gaattagtta cattgttgac tatagccatc ttagtcaaat
     1201 aaaattgttt ttccagtttg cgcatttgta ccatttcata aacttcttta aaagatacct
     1261 ttccctgctt ctcagaattc gctgtaatcg ttacatcata atttctgaca ccgtagagag
     1321 ggctaataaa aaatgagtct cttgctgaca tgaacccatt tttcccgccg cctcgcgcaa
     1381 tactatttag aatttcatca aaaaacacct cgtcgtcctc tttttgatag cagaaaatga
     1441 aaggtgtgat aaatttttga tatttagcta aaggaaaaaa atttttttca gtgtacttga
     1501 taaacttttg aatcatttct tcatcaaaat agatatcatc ccgaactagc acttcgtttt
     1561 ttaaataagt aattaactga attctttctt tattaaaagg gattacccct ttttcgtata
     1621 aatctacgta ttcatcaaaa tagaatggct ttaatgttgt cacagcaaat cactaccatc
     1681 tagcgctgca ttttttgtag gttcgcgctt ttcttctggc aaatagtcag taagttgctt
     1741 aattacacgt tgatatgctt ggtctctcga attatactgt ttagcaactg gtcgttcccg
     1801 ctcgtaaggt ttttgatttt ctgactgaga gaaaagctca taatcacctt tttcggaaat
     1861 atcaatccac atttcatcaa gaagaatttt taagcgtgcc gcttgcgtaa tcaaaccaga
     1921 aacgaccttc tttttatcct tatcaatatc cttaaacaat ttatttagac gcctttcttc
     1981 agcagaaaca agcctatttc tctcttgcag atcagccatc aactcacttc ctttctacgt
     2041 ggggaggggg tcgtgtgaaa atagcatcat ttatttgcag agttgacccc atccaccggt
     2101 tcccaaaaat tttatttctt ctcaaaatct tttgaccggg ggtatttcac tctatatatc
     2161 ttcgctttat actgatttat cagccgtctt gctcgttgct ttctacaata tgtgcaaatg
     2221 gactcaaggt ataatcaatg tctacattaa tattaaatgt ccctgttact tcattagcgt
     2281 tacctattag tctattgttg atagatatat cctctacagt tgttgttcct tcaatgagct
     2341 gactcttaaa tagaaccaac ttatcaatag ccttatcaac catagcgatt gcttctgctc
     2401 gtttgacatc atctctgttt gctgtttttc ttaccaccat tcatcatccc actttctttt
     2461 cttttgttgg ccacgatatt tcatacggtt gtgccttttg ttatggcaat ctttgcataa
     2521 ggttcttagg ttgtttatat ctagagctaa atccggatga tactccagct cctttatatg
     2581 atccacttca agaattgaat catgttgagt tgttagtcta ccctctgctt tacaccattg
     2641 gcactcataa tgatctcgct ctaagcattg acttctaagt ttcctccact cagttgatcc
     2701 atagaaccta gctcttgctt gtttacttga aacatcaatc atttacatat ctctcttgtg
     2761 cccaatgatt tttctatcta agtacttgtt attatgtgaa tcataatatt caatagtgat
     2821 gtcgttagct ccttttggta cttggcattc atattcttcc gtatcccaat gataagtaat
     2881 atcgacaaga cctatcggca tctcatccat tcgttgacct ttataataaa cttctggtac
     2941 tgagtcagta tctcttagtt tgatttctag aaggttcgta ttacttccat catctaacgt
     3001 caattcagca atcctctttt tgatctgttg aattactttt cttcctgtct cacctgtcat
     3061 tggtactacc gtctcatatc cagtaaactc gcaagtaaag cgttcaatag cataagcgtt
     3121 ttgaagtggc ttagcactaa gaacagctgg attattgcta taaaaacgat ctactaataa
     3181 cttccctata ggaatggcat ctaaagctct atcagtaaac actaaagcat tgggataatc
     3241 ttcttgaatt ttgtaagcta gattgggtgc agttacaacg ttataccctt ctctgatata
     3301 atcttttaat ttcatagttt tcctcctaaa aacatctaat aattacttgc taacaacttg
     3361 atgtaatcat agttatcatt catttgatgt acctctcaat gttgtattgg atatactcgt
     3421 ctttccaata gccatgaccg caatatatca gcttgcattt atccacttcg tttggtgtag
     3481 cttctcttag catttcgaca atagagtact tccctttgat ttgtacagaa cgcacaacac
     3541 gcactgaaca atcaccaatg gttcgaggat attcattagt tagtgataca taccagtagt
     3601 ttctcattat gcagcctcct ttatgtaaag aaaaagccta gcacctgcta gactttgata
     3661 cattcatttg attttttgag ataagtatct acatagattt cacctttgtc cccgttcaac
     3721 gtaatttcaa aatacggtgc tccttttaag ctagcgctga ctaatgcttt gctattttgc
     3781 agtgttttgc atgaccagac tatgaaaata ctaaactcag gcactgctcc acttaggtca
     3841 gcaatctcat tttcaattct ttcacgtaca atagccttgc atttttcgat aaacttctca
     3901 tgattcattt tgtacctccg caagcgcata accagctgtt gatgtattgt taaacgtagc
     3961 tgaacgagta actcccgtag ccttcccagt ataaataaaa ctaaatcctg ttgttgttgg
     4021 cttaaaattc tccacttcat aaaaatgata tgtttgtccg ttattagtaa atacaatcaa
     4081 ttccattttt caaaatcctc tcttttttaa aataaaaaga ccactcaaag agtgatctaa
     4141 tatgtaatgc acaggcaggg acgtttccga tcctgtgctt gagtcatttg acgattcatt
     4201 tgtaccgaat cccaaaactc aatctaaccc aaccaattat gtaatagcaa cctacaagat
     4261 gcacaaaacg cgtacgaaat tgcgcacccc tatattttta aaccgccgat gcctcggtta
     4321 ccaaagtcac tggcaaggaa tcgaaccttg cagtttgcac ctaaatttta gatgagcgat
     4381 acacctttcc accacagtga cataatgaca atagacagca actgatgata gataataaga
     4441 acaatttaga ag
//
LOCUS       DACDCV010000137         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDCV010000137 DACDCV010000000
VERSION     DACDCV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.139"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV041_002647"
     CDS             complement(543..2048)
                     /locus_tag="IV041_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8383380.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV041_002648"
     CDS             complement(2045..2731)
                     /locus_tag="IV041_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8383381.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV041_002649"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV041_002649"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8383382.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDCV010000138         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDCV010000138 DACDCV010000000
VERSION     DACDCV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.140"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IV041_002650"
     CDS             690..1658
                     /locus_tag="IV041_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383383.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IV041_002651"
     CDS             1669..2484
                     /locus_tag="IV041_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8383384.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IV041_002652"
     CDS             2552..3262
                     /locus_tag="IV041_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8383385.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IV041_002653"
     CDS             3298..>4425
                     /locus_tag="IV041_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8383386.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDCV010000139         4422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDCV010000139 DACDCV010000000
VERSION     DACDCV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.141"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..121)
                     /locus_tag="IV041_002654"
     CDS             complement(<1..121)
                     /locus_tag="IV041_002654"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383387.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKL"
     gene            complement(262..516)
                     /locus_tag="IV041_002655"
     CDS             complement(262..516)
                     /locus_tag="IV041_002655"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383388.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(709..984)
                     /locus_tag="IV041_002656"
     CDS             complement(709..984)
                     /locus_tag="IV041_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8383389.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(956..1909)
                     /locus_tag="IV041_002657"
     CDS             complement(956..1909)
                     /locus_tag="IV041_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8383390.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2521..4014
                     /locus_tag="IV041_002658"
     CDS             2521..4014
                     /locus_tag="IV041_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8383391.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4149..>4422
                     /locus_tag="IV041_002659"
     CDS             4149..>4422
                     /locus_tag="IV041_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8383392.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1445 a          740 c          777 g         1460 t
ORIGIN      
        1 ttagcttttg gacatgttta agagatactg aaatcccttc ttttaataaa acttgatgga
       61 tctttgtggc accatagcgt tgtttattcg taaaaaaaat tgttgaaatc ttctttttca
      121 acttattatt tctgatttct gtagctgtca acggtttatt cttataaaaa taatagacac
      181 ttctagggat ttctaataac cgacacaaca aagaaacagt gtaatcctta caccattttt
      241 caataaattg aacaagcgaa tttaggtttt ccgagagaat atggctgcgg ctttttttaa
      301 gatttcaagt tcctcattta aacgagcatt ttcttttctc agttcagcta cttcttttcc
      361 agtcagtcct gtggactgat caggtaaata taaattcttc catttgtaaa tcgttgcttc
      421 agataagcca tattctttcg ctaaccctcg aacagaacgt ccagtttgat tcagttcaac
      481 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
      541 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccaaatgt
      601 tgtatacccc caaacaatac acatattttg aaggattagg attaataaag ctaaaaatat
      661 agtaagtata ctaaatatat ttactatatc tagtatactt actatatttt aatcttttga
      721 tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg cgtccattgc
      781 aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc gaatggtttt
      841 tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact ttcgttcccc
      901 taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt tcttcttatc
      961 tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt aggatatgat
     1021 tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca ccatgttgtt
     1081 catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata gcccaccatc
     1141 ttcgatctac ataatcattt aattggatac cattctcacc atacatttct aaccgatccg
     1201 agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt gttttttgat
     1261 agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt ccaatgattt
     1321 gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta ataaaatctt
     1381 ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa tctgcagcag
     1441 ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag atataatcga
     1501 aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta cgacgatcag
     1561 gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca gccataatat
     1621 ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg attgctgcag
     1681 acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa gtagtatttc
     1741 tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta gaaacaatcg
     1801 ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta agcattacaa
     1861 cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata gttccctcca
     1921 atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg atatatctaa
     1981 tatactccaa attgataaga attcagactt tataaggaat atagtggata tactaaatat
     2041 atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt gtgcgttttg
     2101 cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac agataattca
     2161 ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac ataaaaaaaa
     2221 gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg gttaggacat
     2281 gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat taaaagttga
     2341 tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac tcaacagaag
     2401 aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag aacacatact
     2461 atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac gttacaatcg
     2521 atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata caaatatcga
     2581 aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc tatctttgcg
     2641 taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc ggaagaagct
     2701 attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg ctttggtact
     2761 tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt aagtcaaatc
     2821 aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat tattttagcc
     2881 tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca tggttatcaa
     2941 gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt caaggtgatc
     3001 gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa attgtccgga
     3061 gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa taatattgtt
     3121 tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc gatgaagctt
     3181 gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt tcctgagaaa
     3241 aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa agcaaacatc
     3301 gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc tttagcttat
     3361 tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt caacgaacgg
     3421 ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc ttattctggt
     3481 aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg ggtttcacca
     3541 accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt taaaaagcca
     3601 cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt ggcttatcta
     3661 agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga attggcttca
     3721 caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg agaggagaat
     3781 aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc ttcagtcaaa
     3841 acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta ttcaacaagg
     3901 atcacagagg tatttggact caacgagagc ttagtagaag agacatttag acaattatca
     3961 acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg ttgaaaaata
     4021 aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt tgctcccttt
     4081 gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg tatcatggag
     4141 gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg tttatcattg
     4201 gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag caggggactt
     4261 tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag tttcttcatg
     4321 ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg tgtgtgttag
     4381 agcaggagta tattgagtat aaaatagcta acgagttaag aa
//
LOCUS       DACDCV010000140         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDCV010000140 DACDCV010000000
VERSION     DACDCV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.142"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1174)
                     /gene="dltD"
                     /locus_tag="IV041_002660"
     CDS             complement(<1..1174)
                     /gene="dltD"
                     /locus_tag="IV041_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8383393.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLE"
     gene            complement(1177..1410)
                     /gene="dltC"
                     /locus_tag="IV041_002661"
     CDS             complement(1177..1410)
                     /gene="dltC"
                     /locus_tag="IV041_002661"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8383394.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(1455..2660)
                     /gene="dltB"
                     /locus_tag="IV041_002662"
     CDS             complement(1455..2660)
                     /gene="dltB"
                     /locus_tag="IV041_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8383395.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2657..4171)
                     /gene="dltA"
                     /locus_tag="IV041_002663"
     CDS             complement(2657..4171)
                     /gene="dltA"
                     /locus_tag="IV041_002663"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8383396.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(4182..4331)
                     /locus_tag="IV041_002664"
     CDS             complement(4182..4331)
                     /locus_tag="IV041_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8383397.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1372 a          899 c          737 g         1388 t
ORIGIN      
        1 cttctaaaaa cggtctgatc tgttgatctg cagccaacca gcctttccag cctagatgga
       61 tcgtgtcttc cataaaatag ttggtacccg cttgattgac aaaatcagct attcgattga
      121 atccttggct atttaattga aatttgatct ttttggcaaa tccttgtaac atttcttgag
      181 atagtccggt ataatcgctc catttctcgt taaccggtgg gatgatgaac aatacttcgt
      241 tatggttttt tgctaattga tccaagacca gctgaaaatc agaaaattct ggcgaaaaac
      301 gataatccca atttttttgt gactgtttga gctctggctc gtgggctcgg atacgatgcg
      361 tataaaaatc gtttttcaac ccgaatggat tattcgtcgt ttctcgttct ccaatttgat
      421 ttgctaaaat ggataattct gtttcttggt attgttttgg taatcgtttt gactcatgat
      481 cgattttttg ctgtcgatct ttcaacccaa tattgctgaa tacttcatcc tctctcttca
      541 gcatgttcca ttggctgcgc agctgattca aagattcagg taaaggtagc ttcccttttt
      601 tgatcgtttg cagaatcgcc gtcaacgtat cattctcttt tacttttgag aaagttaata
      661 atcttctagc taaatagcga tcagcaggcg ttactttctt cattgaaaac aaccagtcgt
      721 atgtttgaag ttcagaataa tacgtattaa agtaatcagt ttttacacca tttttaacga
      781 accactgagg agaaataata aagacgactt tcttgttttt catagcatct cctgcagaac
      841 gcatcatcat atattggctt aacgattgtg tcccgggtgc tcccagcaaa aatggacgat
      901 agtttcgctg gtatttttct gccagaacag acggatgaaa tggactgatt ctgcttaatt
      961 cacttgagcc aaaaaaagga atatattgtt tttcagataa tgcataattt tttatcgcat
     1021 ctcctttcaa cacgttcact gacatggaag aagaggcacg tctaatcgtt tctggattag
     1081 agatatctac ttgataggag gaggtaaaaa acacaaacag taagccaaca gctaaaacaa
     1141 ccgggccgaa aatagcaaaa agtttctttt tcatactcaa cccttcaatg cagttgcttg
     1201 ttgaatgatt ttattcggtg tgctccaatc ctctcgctcg aattcagata cagggacttg
     1261 gatatccagc tctccgtcta gctcgactaa taattgaaca gatgctaaag aatcaagcaa
     1321 cccttcatca aatagatcaa tatcacgatt ttttcttact tcatctgttc ctgttatttc
     1381 ttctaagatt gataaaattt gttcttccat tttccttttc tcctttgtta tataaattaa
     1441 tatgtcacta ttcgttagct aaaaaaaagt ttatctaaaa atcctgaaaa aatcaggaag
     1501 ctaaaacata ctgcttgaaa tgtcaaaaat acggctaagc catgcgtaaa tttgttagaa
     1561 ggcaactgct ctttatgctt cttcttaaat cggatccatg catctgtcag acagatcaga
     1621 caagcatgat acagtccata tacgatgtag taccacgtca atccatgcca tatacccatt
     1681 agaagaaaca aagcaaaata accgatattt gacattacga tcttgctttt aaaaaatttt
     1741 ttcttcatca tgaagaacat caatcgcata tatacataat cacggaacca aaaggacagc
     1801 gtcatatgcc aacgattcca gaattctttg atattccatg ataaaaaagg tttattgaaa
     1861 ttgattggtg tctgatatcc catcatgtag cttgtaccga ctgcaaataa gctatagcca
     1921 gcaaaatcaa aaaacaaata catactgtac acgtacatat aacctactaa tgcccatgaa
     1981 ataccaccat gctgtaaggc aatgcgtccc actacaggaa gcagtacttg accaaaataa
     2041 taaccgataa tgaatttata taaaaaaccg acaaaaatat tttggacgcc ttttcccaaa
     2101 agatcaacat atttatcacg aacaggcgga tggagctgat ctttttcaaa tctcctatag
     2161 cgatcgattg gtccagaaga gattgttgga aagaacagca gaaactggat ataacgaaat
     2221 ggcttatatg ttttgatcat gccatctctt atttccatga tcatttgtac agatttaaag
     2281 gttaaataag agattcctaa aaaaccaagt aacgatgctt ttccatgttc aaaaaaagga
     2341 gtcactttcg ttaaaaacag tggaattatc gctaaaataa cggcggcaaa aaaccatcct
     2401 gtctgatttt tcctttgccg ataaacaaaa tagccccata caagtatcgt ctgccacaaa
     2461 acatacccaa tcagcgccgc tccctgttgc caataattac ctccaaagct gacaaataaa
     2521 aagaaaagtg tcactgctga ttgataaact ggtaatcttt tttcccatat caataaagat
     2581 agaacgatcg gtacaagcgc aatggctaaa agaaaaaaat aaaacggctc tgcataaggg
     2641 atcatatgag gtaacatcat tcaggattca cctcagctgt taattttttt cgatcgattt
     2701 ttccattaac tgtctgaggc agcagatcga cataatggaa cttctgagga atcatataat
     2761 ccattacaac ttctagtaat tgttctttga tagatttagt caactgaaaa tccggctgtt
     2821 tcgttttgtt ttctaatacg acaaaagcaa ccagctgctg tacttttgtt ccttggtatt
     2881 ttggtacgac gacagcttgt ttgattcggt tgtcttggag cagataatgt tcgatatccc
     2941 cgagttcaat ccggtatcca tgtaatttga cctgctgatc catacgtcct tcataaaata
     3001 aaagcccata ttcatctagt ctacctgcat cccctgtatg gtaacttgct aagtcatttt
     3061 gacaaaagaa cgcagcagct gtcttttcgg gattgttcag gtaacctttt gatacgcttg
     3121 gtcctgcaat aatgatttca cccgtttctc cttttgaaac aggtttacct tcttggacaa
     3181 tagaaacttt cgtgtctttt tttacgtagc caatcggtag tcggtcataa ctatctagta
     3241 catgctggtc aatctgaata cttgaaatag ctacagttgc ttctgttgga ccgtaagtat
     3301 tatatatccg tgcatttggg aatcgatcaa gtagactttc tgcagttttt ttcgtcaatt
     3361 cttctccgca aaataaaaac atgtccaatt ccggcacatt ttctccattg aatgtaggtt
     3421 ccatcaagca aatatccata aatgatggag tagaaaccca cacattcaac ttcaaggctg
     3481 gcaatgtcgc gaataattga cgaaagtctt gaacaactgc tttatttaac ggcttcagca
     3541 ttccgccaga aactaatgca ggataaatag aaaataccga taaatcaaaa gaaaatggcg
     3601 cttgggctaa gaaatgcata ccaggcttga tcccaaagtc ttccatcgtc cattgaacaa
     3661 agctgactag attatcatga ctgatttgta cgccttttgg ttctccagta gtaccagaag
     3721 taaaaataat ataaaaattt tctgtacctt ttacgctatt tctgggataa aatcctactg
     3781 gtatcttctt gatttcttga aattcttttt ttgttatgac aggaaccgaa ggaatatctt
     3841 ctatccagtc cccgatactg atgaccatag atggtttagc cacccgaaag atcgatagga
     3901 tacgctcctt gggtgtatgc tcttcaatcg gcaaataggc atggccagat ttgacaaccc
     3961 ctagaaatga gacaatcatt tcaaactcca aattgccaaa aacgacaatt ggtccatcgc
     4021 cttctacctt tttttctaaa taataagcaa gtgcgtctga tgcttgtttc aattcaccaa
     4081 aagtatgcgt ctcatccgcc tgataagcaa ctcgatcagg ttcatttatt ccccaatcat
     4141 cgattgcttc aataatcgtt ttgatttcca tactcttcct cctaaaattc gttatagata
     4201 aaagttcctc cttgaatatt tttatagtga tacagataaa tcaggaacag cagtactgca
     4261 ctataaaaaa ttgttcgaat gatgaatttt ccccaataat tcaattttgg attagaacgc
     4321 catttgttca ttggattccc tcctcatata acctcacaca aagtgtaact aattttgaca
     4381 aaaagaaaca tgtgtc
//
LOCUS       DACDCV010000141         4380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDCV010000141 DACDCV010000000
VERSION     DACDCV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.143"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV041_002665"
     CDS             156..1862
                     /locus_tag="IV041_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8383398.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV041_002666"
     CDS             complement(2020..2922)
                     /locus_tag="IV041_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8383399.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV041_002667"
     CDS             complement(2919..3623)
                     /locus_tag="IV041_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8383400.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV041_002668"
     CDS             complement(3654..4295)
                     /locus_tag="IV041_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8383401.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1343 a          940 c          759 g         1338 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
//
LOCUS       DACDCV010000142         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDCV010000142 DACDCV010000000
VERSION     DACDCV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.145"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IV041_002669"
     CDS             complement(387..1853)
                     /locus_tag="IV041_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8383402.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IV041_002670"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IV041_002670"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8383403.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACDCV010000143         4326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDCV010000143 DACDCV010000000
VERSION     DACDCV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.146"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..283)
                     /locus_tag="IV041_002671"
     CDS             complement(<1..283)
                     /locus_tag="IV041_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8383404.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..1274)
                     /locus_tag="IV041_002672"
     CDS             complement(732..1274)
                     /locus_tag="IV041_002672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383405.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2051..2241)
                     /locus_tag="IV041_002673"
                     /pseudo
     CDS             complement(2051..2241)
                     /locus_tag="IV041_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2361..2723)
                     /locus_tag="IV041_002674"
     CDS             complement(2361..2723)
                     /locus_tag="IV041_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383406.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="IV041_002675"
     CDS             complement(2727..3035)
                     /locus_tag="IV041_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383407.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="IV041_002676"
     CDS             complement(3044..3463)
                     /locus_tag="IV041_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383408.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="IV041_002677"
     CDS             complement(3456..3689)
                     /locus_tag="IV041_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383409.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="IV041_002678"
     CDS             complement(3686..>4326)
                     /locus_tag="IV041_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8383410.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DACDCV010000144         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDCV010000144 DACDCV010000000
VERSION     DACDCV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.147"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IV041_002679"
     CDS             complement(65..1285)
                     /locus_tag="IV041_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8383411.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IV041_002680"
     CDS             complement(1305..1910)
                     /locus_tag="IV041_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8383412.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IV041_002681"
     CDS             complement(1959..2891)
                     /locus_tag="IV041_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8383413.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IV041_002682"
     CDS             complement(3312..4172)
                     /locus_tag="IV041_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383414.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDCV010000145         4262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDCV010000145 DACDCV010000000
VERSION     DACDCV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.148"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..388
                     /locus_tag="IV041_002683"
                     /pseudo
     CDS             1..388
                     /locus_tag="IV041_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(514..1593)
                     /locus_tag="IV041_002684"
     CDS             complement(514..1593)
                     /locus_tag="IV041_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8383415.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1696..2136)
                     /locus_tag="IV041_002685"
     CDS             complement(1696..2136)
                     /locus_tag="IV041_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8383416.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2193..2603)
                     /locus_tag="IV041_002686"
     CDS             complement(2193..2603)
                     /locus_tag="IV041_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8383417.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2783..3217)
                     /locus_tag="IV041_002687"
     CDS             complement(2783..3217)
                     /locus_tag="IV041_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8383418.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3317..4165)
                     /locus_tag="IV041_002688"
     CDS             complement(3317..4165)
                     /locus_tag="IV041_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8383419.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1532 a          797 c          700 g         1233 t
ORIGIN      
        1 cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
       61 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
      121 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
      181 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
      241 caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa
      301 caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa
      361 aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca
      421 aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg
      481 gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag
      541 atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc
      601 gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata
      661 aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat
      721 tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga
      781 ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact
      841 taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc
      901 gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc
      961 aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc
     1021 actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa
     1081 acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc
     1141 aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt
     1201 atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac
     1261 ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag
     1321 atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg
     1381 gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag
     1441 caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac
     1501 agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac
     1561 ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa
     1621 gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc
     1681 gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg
     1741 ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt
     1801 ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt
     1861 tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt
     1921 ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac
     1981 tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt
     2041 cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg
     2101 aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta
     2161 tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact
     2221 tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat
     2281 tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca
     2341 aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag
     2401 atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct
     2461 gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc
     2521 aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt
     2581 ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa
     2641 ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa
     2701 agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca
     2761 aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa
     2821 aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca
     2881 ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat
     2941 ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg
     3001 tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat
     3061 ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg
     3121 cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat
     3181 catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt
     3241 cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc
     3301 ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg
     3361 ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc
     3421 gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc
     3481 cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga
     3541 tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt
     3601 cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt
     3661 ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa
     3721 tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc
     3781 tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa
     3841 tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag
     3901 gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag
     3961 ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag
     4021 cagcttccag taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta
     4081 aaaacatatt gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc
     4141 atataaattg tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta
     4201 tcttattata acttactttt caccttaagt taactttaat taataaaaaa attaggctag
     4261 tc
//
LOCUS       DACDCV010000146         4241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDCV010000146 DACDCV010000000
VERSION     DACDCV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.149"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(9..905)
                     /locus_tag="IV041_002689"
     CDS             complement(9..905)
                     /locus_tag="IV041_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8383420.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(898..1578)
                     /locus_tag="IV041_002690"
     CDS             complement(898..1578)
                     /locus_tag="IV041_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8383421.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1579..2361)
                     /locus_tag="IV041_002691"
     CDS             complement(1579..2361)
                     /locus_tag="IV041_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8383422.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2354..3271)
                     /locus_tag="IV041_002692"
     CDS             complement(2354..3271)
                     /locus_tag="IV041_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8383423.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3435..3758)
                     /locus_tag="IV041_002693"
     CDS             complement(3435..3758)
                     /locus_tag="IV041_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8383424.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1247 a          931 c          745 g         1318 t
ORIGIN      
        1 ttgtataatt aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta
       61 gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg
      121 ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca
      181 acagaggttt gcgttcctgg tttgatagga ttggccactg ttaatcgaat gaatggatcc
      241 tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata
      301 ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt
      361 tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact
      421 tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct
      481 attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg
      541 atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca
      601 taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt
      661 ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc atccaaccat
      721 gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga
      781 ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc
      841 ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa cagccattta
      901 atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag
      961 cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc
     1021 ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa
     1081 aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac
     1141 atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt
     1201 tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat
     1261 atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct
     1321 ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc
     1381 taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg
     1441 atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct
     1501 ctgttcaaga acctcctcca gtaaggaatg aacagcttca tcgtcttcga taatcaaaat
     1561 agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca ttcctagagc
     1621 tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt
     1681 tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa
     1741 ttccaagcct ttgatttttg cgaaaacacc gggaatgaag gccgttccca tgatataaag
     1801 aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac
     1861 agataatgtc cctaatgtca gcagaagcaa tacaggaaat acttttagaa cataatttcc
     1921 atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caagcatggc
     1981 tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga
     2041 cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg
     2101 aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc
     2161 cattttttct accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt
     2221 taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag
     2281 tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc
     2341 agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca
     2401 gtttttgccc aactaggtga ataccatcca caggaacttt tgcttctacc aatgcttgat
     2461 tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta gattgtacaa
     2521 aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt
     2581 caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca
     2641 agtgtccttc atgcaaaata ccatagtgag tggcgatctg tgacaattca tctagcaagt
     2701 ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca
     2761 tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg
     2821 gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct
     2881 ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc
     2941 tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg
     3001 gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg
     3061 tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata atagttctca
     3121 taaaggtaga cttacccgct ccattttccc caatcaatcc atagatcatt ccacgtttga
     3181 tttcgattga tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg
     3241 ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat tgatgagtga
     3301 agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc
     3361 caattgaatg tcaaaaaaaa caatcggaca atacgtgtct ttcctattat caggcagttt
     3421 gttatacgct tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt ttctctgtat
     3481 tcctcttcac tgaaaatttt tgcttctttc agatcaagct tgagtaattt taccagaccg
     3541 gttataagga taactatagc aagactccct cccagaaaaa ttagactgtc tcgcagacgt
     3601 ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc
     3661 accatggtaa tcgttgttat cgttgacata agtcgtcctt gtatatgcgt tggaacttct
     3721 cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat
     3781 gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa
     3841 aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca
     3901 caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg
     3961 aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt
     4021 tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt
     4081 aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta
     4141 ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac
     4201 tcgaacgata atcgcaatta acgttttgag aactgtgaag c
//
LOCUS       DACDCV010000147         4240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDCV010000147 DACDCV010000000
VERSION     DACDCV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.150"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(100..912)
                     /locus_tag="IV041_002694"
     CDS             complement(100..912)
                     /locus_tag="IV041_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383425.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1075..1182
                     /locus_tag="IV041_002695"
     CDS             1075..1182
                     /locus_tag="IV041_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383426.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1240..2307
                     /locus_tag="IV041_002696"
     CDS             1240..2307
                     /locus_tag="IV041_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8383427.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2449..2652
                     /locus_tag="IV041_002697"
     CDS             2449..2652
                     /locus_tag="IV041_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8383428.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            2712..3914
                     /locus_tag="IV041_002698"
     CDS             2712..3914
                     /locus_tag="IV041_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8383429.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1412 a          676 c          879 g         1273 t
ORIGIN      
        1 taacaggggg ctaacaaaga acaggaaact gtgacaacta cccattgggg agtgtcctag
       61 cagtattttc tttgtcacgt gcttatcaat tttgtcacct cattttgatg atggatacgc
      121 tgcatttaac aggcgttctt tctcgacttc catcacgaat ggtagataaa gatattgctg
      181 ttctagtaga tattgattgt cctctgtgta ctgataagaa gcggtttctt ctgaatagac
      241 caacggggca attcgcacat cgctggtcaa ttgattaatt gagtatagaa ggatggcttt
      301 ctcacctagt tccttgggta aatgtttccc aaaataggaa taggattttt gatttgctaa
      361 atagacgagt atgcgatcca cactaaagtt ggagaaggta aagggcatca tgtgcagaat
      421 atgattttga ttatcatgtt gcaaatagag ctgggcttcc ttaccaaaat tagttgaacc
      481 aagataaagc tgcttttttt catcgataca ttggaaagta aactggtctt gttcagttgg
      541 tggaatgaga tttgtaataa aaaattcaat cattcgatcc atttcttttt cactaacaag
      601 gtcagcgtgt ttgatttcgt ctactatgag accttcaatc ttaggatcaa gcttacctgc
      661 ctttttttgg agcatttcta aaatgtattg attctcttga tcaccataca aaagatattc
      721 attggtgaca tgacctatct ctgcgatttg gtgaagggtt tcggtttttg gaaggttgat
      781 cccacgttcc cagttattga ctgtacttcg cggtaaatca ccaattagtt tgccaaattt
      841 ctccatactc aactttaatt cttggcgaat ctgccgaatt cgattgccga ctgcttgctt
      901 gtctatctcc atgaaaactc ctcctcagga taagttctta aatttagtat acgtgtgtac
      961 ttaaaaaaag tcaaaacata ttgacttaaa ataaatacag gcgtacaata aaaatgtact
     1021 taaaataagt tcaataggtt ctttgacaag taaatagcag aaggaggttt gtcgatgaac
     1081 caagttaatc aacatgatct cggtgaagcc attcgagttt ctcgagaaga aagggggtgg
     1141 acacaacgat atttagcaga aaaagtgggg atttcgagat agttattatc gaaagtagaa
     1201 aaaggaacga gacaattaag tgaagaaaaa ttgaatcttg tgttggatag tttgcaagaa
     1261 gaggtcgttc cagttaaccg tgttttgatt gattatctaa ccattcactt tttctcgaat
     1321 caacatttaa agctaattga agagattatt gaaatgccca ttgaacgttt tgaggaactg
     1381 gattatgcgc caaagggcta tattggccaa tacgtgtgga atcaagtaat cacgattcgg
     1441 tattccattg acgatgcggt taaaggaact gtgatggagt tttcaggtca aggctgtaaa
     1501 cacttagcaa gatggctaaa aacacgaaaa acaaattggc aacagttttt tcgtaaggta
     1561 ttagattatc aggggaactt tacacggatt gattttactt tagatgattt tgtggggagt
     1621 ctcagcattc cagaacttaa gcataaggta atactaggcc atgtgtggac cacttttcaa
     1681 gtgagtgaat ctcatggcgg tacggatatc ataaacaatg aatcaaatgg tgagaccttg
     1741 tatttaggct caaagaagag tcagtgtcgt ttttgctttt atcagaagga ccatgaacaa
     1801 cggaagcgac gaggaattcc tttagaagag gcaaaagtaa agaatcgctt tgaactacgg
     1861 tatcgaaaag agaaagcgca aagcttggca aagattattt caaaaaccca tgatttaacc
     1921 aaactgttct ttgagttact taatggagca atttgttttt atgaccgtga tccaaatgat
     1981 ctaggtgcaa aagttgatgc caaatgggca gcctttatta gcaattatgg cgcaatcacc
     2041 atttctttag atacaattcc tcaaagtttt gagaaaacta tgagttggtt aattcatggt
     2101 gtttcaccat cattagtttt tattgcacag gtcgatcaaa cgtttcattc ttccttgttg
     2161 cctttgatta ttgaacaagg agaaatgaat ccaagacaga tcaaacttct ccaaaatatg
     2221 aaggcagacc cggagtatta tcaggaggag gttgagtatt atgaacgaaa attacaaagt
     2281 atatcaaatg agaaaacaag ctactaaaaa agcaaagtac tgcttacaca acaagttgtg
     2341 caacgtactc cgcctcgaca agacgagtat aacacaacca tcgaatgaga aacaatagat
     2401 tataaagagg attattttaa aaggagtaaa gaatcaggag gtgagagaat ggaaacaaaa
     2461 tgtgatttca tggtaaatcg tgcaattttg atagagatgg gctttaaacc tagtcaagct
     2521 gcacgaatga ttaaagagag caaggcatat cttgcaagga ttgaaggtat tgatttttat
     2581 aataatcgcc aagttggtgt ggtgccatcc cgagtaattg aacatctttt tcacattcaa
     2641 gttgcggaat aaacagtaca agcgcggtac tattaaagta gcttttctca agtacaggga
     2701 ggaatggaac gatgccaaaa attaaaaata tttaccaaga taaaaagaca aaaaaatggt
     2761 tcttccgagc gtatctaggt atagatgaaa atggaagaaa ggttcagaag actaaacgag
     2821 gttatgcttc acaaaaagat gccaaaattg cttatgacaa gtacatggaa acacatgatt
     2881 ttaacaagac aattcctaat caactgtgtt ctaccaatca aatgactttc gaagagtttt
     2941 acaaggatag gtttgtaaaa tggtacgaga gacaggtcaa aagtcaaaca tatgagaatg
     3001 cgcaattcat tttcgaaaag cgtatgcagt ttttctatca ttttcgtgtt cgagatatta
     3061 caagtcacga tattgaggat tggttatttg aactaagcca gacagagtca cgtaattcac
     3121 ggaaaaaagt taatggcgaa acgttatcaa aatcttatat caatcggatt cgagggcacc
     3181 taaaaattgt attggatcgg gctgttaaag agggggtgat tgctaaaaat cccgttgaag
     3241 atgtttcttc gcttccttct gagaataaga aagttgagtt ttgggaacac agtgagtttc
     3301 aaaaagtgat gaatgaattg aaggatgata agattcaaac ccaccatcgg aagattgtgt
     3361 atgaaatgct cttttatact ggcattcgga ttggagaact tgaagctttg tcttggtcga
     3421 atgtagattt tgagaaaaac acgataacga ttgagaagac attgatttat aatacaaaag
     3481 atgattggta cttttcgact cccaaaacta aatgtgctta tcgaacgatt gggttgggga
     3541 agaagctttc cttaaaactc agacagtggt atgacttgca aaaacgtatt ggaaattttg
     3601 aatatgttgc tcagttggac ggaacattta cgccacctta ttcttttgcg aactggttaa
     3661 aagaagctgc taaaaaagcg ggtgtcaaac cgattaagct tcacgcgcta cgacattctc
     3721 atgttgcatt tctaattgag cagaatattc aaccgctatc tattcaagaa cgattgggcc
     3781 atgcgaatat tcaaatcact ttaggaactt acggtcactt atatgcaaag tcagatcaac
     3841 aagtggtaag tgcgattgat aatgttcaag agggcgctat tctttcaaaa aatgacaatt
     3901 tattaccagt ctgagttttc cacggttcta ccataagaca aataaatgct tttgcaccag
     3961 tgttttcagt cgaattgctc ctgttcaaga tgtatcggaa atagcgagaa aataagcaaa
     4021 tttgcataac tataataaaa aggacttagt tttgatggga aatttttcaa aattaagtcc
     4081 ttttttatgt ggttgaaacg ggtatgatca ttttaaggaa gtgatctaag atgaaaggac
     4141 gactgaacaa ttatcatatt agaaaagctt tattgtattc cacaaacaaa ttactaagaa
     4201 taacaaatta gtttttgttt gttcttattc cctattgact
//
LOCUS       DACDCV010000148         4196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDCV010000148 DACDCV010000000
VERSION     DACDCV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.151"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            594..926
                     /locus_tag="IV041_002699"
     CDS             594..926
                     /locus_tag="IV041_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383430.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            1051..1308
                     /locus_tag="IV041_002700"
     CDS             1051..1308
                     /locus_tag="IV041_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8383431.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1540..1944
                     /locus_tag="IV041_002701"
     CDS             1540..1944
                     /locus_tag="IV041_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8383432.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1963..>4196
                     /locus_tag="IV041_002702"
     CDS             1963..>4196
                     /locus_tag="IV041_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8383433.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILG"
BASE COUNT         1537 a          571 c          772 g         1316 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta
     4141 aatccacatc tagaagatgt agaattcttt tcaattgaag atatgatttt aggaat
//
LOCUS       DACDCV010000149         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDCV010000149 DACDCV010000000
VERSION     DACDCV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.152"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IV041_002703"
     CDS             <1..106
                     /locus_tag="IV041_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383434.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IV041_002704"
     CDS             970..1182
                     /locus_tag="IV041_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383435.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IV041_002705"
     CDS             complement(1460..3259)
                     /locus_tag="IV041_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8383436.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IV041_002706"
     CDS             complement(3383..3979)
                     /locus_tag="IV041_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8383437.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDCV010000150         4120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDCV010000150 DACDCV010000000
VERSION     DACDCV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.153"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IV041_002707"
     CDS             <1..370
                     /locus_tag="IV041_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383438.1"
                     /translation="DNIVPGDPMSVMALTIAKPADCKLDIVKISDVKGIMETSPNDNY
                     MILIKGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAVFLTPEELEATKKILDQE
                     VKIFVQQVPSSSIENVDFSK"
     gene            384..1133
                     /locus_tag="IV041_002708"
     CDS             384..1133
                     /locus_tag="IV041_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8383439.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1133..1963
                     /locus_tag="IV041_002709"
     CDS             1133..1963
                     /locus_tag="IV041_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8383440.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            1963..2610
                     /locus_tag="IV041_002710"
     CDS             1963..2610
                     /locus_tag="IV041_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8383441.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2708..3484)
                     /locus_tag="IV041_002711"
     CDS             complement(2708..3484)
                     /locus_tag="IV041_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8383442.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            3628..>4120
                     /locus_tag="IV041_002712"
     CDS             3628..>4120
                     /locus_tag="IV041_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383443.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLE"
BASE COUNT         1375 a          610 c          736 g         1399 t
ORIGIN      
        1 tgataacata gttccaggag atccaatgtc tgtgatggct ttgacaattg ctaaacctgc
       61 tgattgcaaa ttagacattg ttaaaattag tgatgttaaa gggattatgg aaacatcccc
      121 aaatgataat tacatgattt taattaaagg acctaaggaa gcttttgaat tagttcaaaa
      181 tttaccagaa attaaagaaa ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca
      241 atatggaaag gcagtctttt taactcctga agaattagaa gccacaaaaa aaattcttga
      301 tcaagaagtg aaaatatttg tgcaacaagt cccatcttca agtattgaaa atgtagattt
      361 tagtaagtaa ggggggaaaa taaatgttat taaaagccat tttattaggt ttagttggtg
      421 tggtagctgt tattgactcc aggttaattg gtcgacaaaa tattggtaga ccactaattt
      481 tgagtacact tgccggactt gtgctaggtg acttgcatac agggattgtt cttggggcat
      541 ctttagaatt aatttctatg ggattcgtct caattggggc ggctggacct cctaatatgc
      601 aacttggtag tattattgcc actgcttttg caattttaac taatagtagt acagaaacag
      661 ccttaacaat agctatccca gtggctgtag ctggtgaatt tatgagtatt atcatgcgca
      721 tgataattgc tcaatttgga catgctgcag ataaagccat tgaaaataat aaatttagaa
      781 aagctcaaat tattcatatt tattggtcat ttatttttaa tgcttttgtt tactttattc
      841 caatcttttt aacagtatat tttggtgctg acgttgtggc agatttagta gataaaattc
      901 cagaaatgat tacagacgcc ttgactgtct ctggaaatat gctttccgca ttagggtttg
      961 caatgctatt aagcacaatg ttgagtaaaa aattatatcc ttactttatt tttggattct
     1021 ttattgttgc ctattcaggt ctttcactaa ttggcgtgac aatttttgca gctctgattg
     1081 cctttgtaat ggatcaagtt aaatatggaa aaagagaaga ggcgcatggt taatggaaaa
     1141 tagtacagaa aaaaaacgtt taatcacaaa aaaagatctt aataagatgt gtcttcgttc
     1201 atggacaatc atgtgttcgt ggaattatga aagacaaatg catatgggat ttatgtatgg
     1261 gatggcgcca attttaaata aagtttataa aaatgatgaa gagaaaaaaa gagaagctta
     1321 tactcgtcat atggcatttt ttaactgtac gcctcaaatg acttcattta ttatgggact
     1381 ttgtgcttct atggaagaac aaaatgctca agcagaagaa ggaaacttta atactgaatc
     1441 tatctccatg attaaaacca gtttaatggg tccttttgct ggaattggtg attcattttt
     1501 ccaaggtact ttaagaatta ttacatttgg gattggattg tcttttgcta agcaaggtag
     1561 tattttagga ccgattttag ctgttggcct ttttgctata ccttctattt tagctgtttt
     1621 ttacggcggt aaattaggat atgagtctgg caataaatac cttactaaat tgtatcaaga
     1681 aggtcttatg gatcgagtaa tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg
     1741 aatggtggct agcatggtgc caattgtcac ccctttaaaa ttcactacag ggggaacaga
     1801 acttgtttta caagacttac ttgattccat tattcctgga ttattaccac ttgtatgtac
     1861 tttgggaata tataaattga ttaagaaaaa tgttagtaca aatatgctct tattaggttg
     1921 tattttggca gggtttgttt taagtataat aggtatctta taatgattaa ggctctaatt
     1981 tttgatatgg acggagtttt aattgatagc gagattatgt atcaaaagtg ggtatttaat
     2041 tttttaaaac aagaaaattt aagctatcct ttgtattctt atcaaaaaaa gattggaact
     2101 acagcaagta tttttgatga tatagaaaag tacaatgagg gatgtaacag tttagaattg
     2161 aagggaaaat ttactaaata ttggaaatcg caaaagattg attacttgga tatttttcct
     2221 aatatactaa ttgaacagct tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct
     2281 tcttccagtc caatggatac tataaatgaa gttgttacca cttgtaaaat agagtcatat
     2341 tttgattgtt taattagcgg aagagatttg ccagaaagta aacctaatcc tactatattc
     2401 cttaaagctt cagaacagct cagaattcca gttgaagaat gtctggtgat agaagattct
     2461 tataatggta taaaagctgg taaaagagca aatatgaagg ttcttgcaat taaagataaa
     2521 cgtttttctc aagatgtaag tctagcagat gatgttattt acgatattaa atatctgcga
     2581 caagaagtac aggtgaaatt tggaatataa ataagttttt tgatgtattt agagaatatt
     2641 agtaaaaata aaaatcatgc ccaaatctaa tcaaaattga ttgctttggg tatgatttct
     2701 tttaatatca atctattact ttaggatctg aatcattttt atgcaccgta tctaaagtga
     2761 tattttctgt gacttcaata acataactaa ttctatcaca acgaactgtg gattgagaat
     2821 aatgaaatgg ttgattattt ttatctttgc caaccttttc taaaataaat agcggatctc
     2881 ctactataca ttgcaataag tcggaaagct caggtgttgc agataaaccg tttatttcca
     2941 taatagagct cgtagctttg acatcatagt catcttctag aagtttatat aatgaagtat
     3001 attggctacc tttttcaaat aaagttggaa atttttcttc tgatgcgaaa aaattatcaa
     3061 ttcccatcgg ggcaccgtct tcccacatta aacgtttaat attgtaaact ctatcagtta
     3121 cattgatttg taatttatta gctaaaaact catcagcctt tataatttta gaatgtaaaa
     3181 cagtatatct aattttatgt cctcgggtag aaagagaatc gctaaatcca tgaactccag
     3241 ctaattttaa actttctttt ttttgagtta caaaggtccc tttccccctt aatcgttcaa
     3301 ctgttccact ttctgctaaa agtttaaaag cttttcgaat ggtaatctta cttactccat
     3361 aaattttaca taattcttct tctgtaggta attgctctcc tggaagatat tttttttcac
     3421 gaatatcgtt acgtatagaa gcttctactt gtaaatataa tggtctatta tcttgaaaat
     3481 tcatagtaca ctccctaaaa agttatactt cattatatca caatttatta aaacgtcgat
     3541 tgtaaaatta agctagacaa ctaaaaagat catttgtgct acactcaaaa tagttccgac
     3601 caaagaatta ataaggagtg agtacaaatg acttacaccc atcttacaac agacgaacta
     3661 gtaatcatag agtcttattt caatatcgag aaacctgtcg ctcttgtcgc acaaagttta
     3721 aatcgctcta gacaaaccat ttataatgtt tattcgtttc tcaaacaagg aaaaaaagtg
     3781 ctagattatt accgtcaata caaacaaaat aaatctcgtt gtggacgacg ttcaaccgtt
     3841 ttatctgatg aacaatcaac atatgttcaa gaaaaggtag ctcaaggatg gtcacccgat
     3901 gtgattatcg gccgtggcga gtttccaatt gattgttctg ttcgtacact ctatcggatg
     3961 tttaaaaaag gcctttttga cacacagtcg ttacctatga aggggaaacg taaaccgaat
     4021 aatcatcaag aaaagcgtgg aaaacaggcc tttcgcagaa cgattcatga acggaacagt
     4081 ctctatccac acctgactaa agaatttggt catctcgaag
//
LOCUS       DACDCV010000151         4108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDCV010000151 DACDCV010000000
VERSION     DACDCV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.154"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            621..1571
                     /locus_tag="IV041_002713"
     CDS             621..1571
                     /locus_tag="IV041_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8383444.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1592..2125
                     /locus_tag="IV041_002714"
     CDS             1592..2125
                     /locus_tag="IV041_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8383445.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            2761..3294
                     /locus_tag="IV041_002715"
     CDS             2761..3294
                     /locus_tag="IV041_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383446.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            3306..3509
                     /locus_tag="IV041_002716"
     CDS             3306..3509
                     /locus_tag="IV041_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383447.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3831..>4108
                     /gene="mobC"
                     /locus_tag="IV041_002717"
     CDS             3831..>4108
                     /gene="mobC"
                     /locus_tag="IV041_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP8383448.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWC"
BASE COUNT         1466 a          685 c          773 g         1184 t
ORIGIN      
        1 cttacaaaat aaaatctgtg tggtgttttt aagtatccct tattttcggg ttgttagggc
       61 ttgccctgac cgcctgtaag gcgctttaag aacaagataa ggttttttac ccaagactgt
      121 cttaaaacga cttagacgag ctatcagagg cttcccccac ctccgttact gacttgtttt
      181 tagcaaagga gcctttaggc gactgcgcag tacaatgtga gagcatagcg atcacacttt
      241 tttagcgacc gtagggagca aggttttgaa ttggcagttt tgaaaaatgc gcgaacctgt
      301 tggtagaaaa aagaagtggt ttttaggcgg gaaattttgt caccctcttt tataatctct
      361 ttttaaacct ttttttaaac ctctttaccg tcggctgtgt ccttactcct tcaatggata
      421 cagcgtttta taaactacat tttgtctgct tttaaactac attttgtctg cttttaaact
      481 acattttgtc tgcttttaaa ctacattttg tctgctttta aactcttttt ttgcactcac
      541 gtatgttata atatgcatta tcacagacaa aacgttgttt ataacgtctg tatccattga
      601 aggagtaagg aggaatatct ttggcaaatg aaatcgtgaa ataccatcat gaattaaata
      661 ccattccgtt gcggaaattt acaccagttg aaatgaatct atttttctct attgtgtcta
      721 gaatgcgcga aaaaggcgat aaaacagtcc gtcttacttt tgaccaacta aaagatttaa
      781 gtaattataa agcaactgct aataatcgtt tcattaatga tttagaaatg acttatgata
      841 aattaatgga tttacgtttt ggtaaaagaa gtgctaatgg gctcgatata gatagattcg
      901 ttatgtttag tgagtttaaa atacgtggaa ctgctaaaga tccatatgta gacatacaag
      961 tttacgaaaa agctttacct ttactaaaca atttagatga gtgggttcgc tattcactcc
     1021 aacaatttaa cgaattacaa agtagctatg caaagactat gtttcgatta ctaaaacaat
     1081 atcgaactaa aggttttgct tatttttcta aagaggattt tcatgaacta ttagatattc
     1141 caaaaagtta ctggaacaac caagcaaacg ttgataatcg tgttataaag cctatacgac
     1201 aggaattaac cgcaatcttc aaaggattat caatcaaaaa gaaatacgga aaagggcgag
     1261 gaaaacctgt cgttggctat caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt
     1321 ttaataaaca tgcaccaacc aaagaagaac aaaataaagc attggcttta gctgataaac
     1381 aaaaagaata cgaagaacct gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag
     1441 aacttgaact cctccaacaa atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac
     1501 aaacagagtc cgcactttcg aaaaaagaga aaaaaagttt attatctgat ttagataatt
     1561 tattccagta gcaacctagg aggcaaccag tatgcaacca caagaaacct ttacaataaa
     1621 ggaattgtca gaacttttta agatgagtag gcaagcaata tcgaaacata tccaaaaatt
     1681 agattcttcc atgatagcta aaaatgagcg tggttataag gttgtattgc gttctggagt
     1741 attgcaactt gctcgcaact taggcaacca aatgttgcta gataggttgc acgagtacga
     1801 acaagacaac gataaaaatg ttaatatacc aagcaaaaac gtggttacaa acaagttgct
     1861 agatcagttg cgtgaagaaa atgaattttt acgagaacaa cttgccctca gagagcaaaa
     1921 taattctcat atacaaaaac tactcgacca gcaacaacaa ctcaccctac aagccaacaa
     1981 acaaatcgaa aaactgcaag aaaaactaca gttgacttat acagaagaag ctccagagaa
     2041 tgactcaacg actctttctg aagcaacaga aacaatagac aaacaaattg atactgaaaa
     2101 aaaatggtgg caactttgga aataactgga gggaacattc atgacaagaa aaaatcttct
     2161 attcggttct attttactag gaattctagg attcagtccg atgttattgt cagtgatcaa
     2221 taaagggaat aaacatggtg aattgaaatt gaatccaaat tattacaaca gtcctaacga
     2281 agaattgttt gtaaaagaaa cagataaata ataaaaaaga ctgctctttc gagcagccag
     2341 ttaaaaaata atgagtgttt attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa
     2401 atttatcata ttttattaaa aataacaacc cagtaaaaaa tgactggtaa aaattcacaa
     2461 agaaacggaa agaagcaacc gcagaaaatc aagcttaaaa agagcttttg acctagacca
     2521 atcgaaaatc acaaagtgag cgcctgtatc tattgctaca ggcgctactt ggcatagtac
     2581 acattatgtc aacaagaatt taatataaca tattttccta atgcattcta actataccac
     2641 attatcaaaa atataaatta tctttactaa ataaaatatt gacaagccta taccttttgt
     2701 tttatgattt tcacaaagaa aacgttttct atatattttt ctgtatcagg aggaaattct
     2761 atgaaaaaat ttactttagg ggtagctttg ttaactattg gggcagttgt tttaccgtca
     2821 actactatag tttatgcttc agaaaattcc caagtggtat cattaaacga aaacaatgct
     2881 actgaaaatg tagttattga gtacactgaa gatggtatgg tcgtaactaa atatgaggat
     2941 ggttatagtg cggaagattt tgcaaattct tcagatatca tgacaagagc tgcagttgca
     3001 agatggggca aaaaggaata tacaaatatt gctgtaacca ctggagtagc tgctaatgct
     3061 attaatgctg ccttttgggc aggagggaaa gctagcactt ctatgtttgg tatcccaggt
     3121 tgggcaattg gtgctttgtt aacaggtgca agttggacta aaaaaggtgc ttctccaggg
     3181 aatgcagttg caaaagaatg ggacaaaaat ggtaatggct ggattggatt ttattaccaa
     3241 aggggatatg atgcagctgg aagagttgta gctactagat attcaacgtt ataagttaag
     3301 gagtgatgtt tttgatatct aaatcattaa aatcattaaa aacatttact aaaaagaatc
     3361 cgcaaatagc tactattcgt atttttatat ttgctatagc tgttgctata attgttaata
     3421 tgctaagcgg agggcaatta tacaaatctg gtttattttt agtgttaatc ttagttgtct
     3481 tagacataat aaagtctaaa aaaaaataaa tcgatacatt ttagaagaat agggttgttt
     3541 gaatattaca aagcaacaat ttcaccttcg gtgaaataag cgaaacttta caaaaagtgg
     3601 ctagcaaatg ctagcctttt tttgtattgt ggagcgtgga ttttgagggg gattcaaggg
     3661 ggagcttgcg ctcctttgac aagcggttat ttgcaccaaa tacggaggta tttggctagc
     3721 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
     3781 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     3841 aacatcaaaa tttggctagc gatcattcta aaaaatatac ctatcgatcc gagccaaaac
     3901 aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca gctgaagctt
     3961 tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac
     4021 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     4081 atatcaatca aattgccaag tggtgcaa
//
LOCUS       DACDCV010000152         4047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDCV010000152 DACDCV010000000
VERSION     DACDCV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.155"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IV041_002718"
     CDS             11..733
                     /locus_tag="IV041_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8383449.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IV041_002719"
     CDS             791..2830
                     /locus_tag="IV041_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8383450.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..>4047)
                     /locus_tag="IV041_002720"
     CDS             complement(2939..>4047)
                     /locus_tag="IV041_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8383451.1"
                     /translation="IGSHISRRLGIPAVIGQLLVGVLLGQAGLNWVKPDILVHDFSEI
                     GVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLTGKAFQVSGNEAIFFG
                     IILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLVLSFSLSFLTGEASSN
                     ASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIIIVSLVICLGMSYVADL
                     VGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFSEQL
                     LFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQSQL
                     IEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1464 a          754 c          799 g         1030 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcg
//
LOCUS       DACDCV010000153         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDCV010000153 DACDCV010000000
VERSION     DACDCV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.156"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..606)
                     /locus_tag="IV041_002721"
     CDS             complement(64..606)
                     /locus_tag="IV041_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8383452.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="IV041_002722"
     CDS             741..1130
                     /locus_tag="IV041_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383453.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="IV041_002723"
     CDS             1134..1898
                     /locus_tag="IV041_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8383454.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="IV041_002724"
     CDS             2027..2974
                     /locus_tag="IV041_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8383455.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="IV041_002725"
     CDS             3104..3844
                     /locus_tag="IV041_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383456.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1346 a          687 c          815 g         1193 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3961 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     4021 agatcgtttg caaaaatagt t
//
LOCUS       DACDCV010000154         4008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDCV010000154 DACDCV010000000
VERSION     DACDCV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.157"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..293)
                     /locus_tag="IV041_002726"
     CDS             complement(<1..293)
                     /locus_tag="IV041_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383457.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(265..1464)
                     /locus_tag="IV041_002727"
     CDS             complement(265..1464)
                     /locus_tag="IV041_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8383458.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1594..2970)
                     /locus_tag="IV041_002728"
     CDS             complement(1594..2970)
                     /locus_tag="IV041_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8383459.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2970..3626)
                     /locus_tag="IV041_002729"
     CDS             complement(2970..3626)
                     /locus_tag="IV041_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8383460.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3636..>4008)
                     /locus_tag="IV041_002730"
     CDS             complement(3636..>4008)
                     /locus_tag="IV041_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8383461.1"
                     /translation="SLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSI
                     ISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLS
                     VVFASSLMLIKKPKEILSKIS"
BASE COUNT         1259 a          739 c          548 g         1462 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gacatcatcc ataaaatgga ctttgtctac
      361 agtctgcgca ttctcattag agaacactcc cttttttgtg acttttctga agctagagtg
      421 aatactttca actgcattag tagtgtacat gatttttcta atggcgctgc cataatcaaa
      481 gagttgttca acatgagaaa agtttcgttt gcatacatct actgcaccag aataatgaga
      541 ccaccgattt tgaaaactgt cgaaggcagc gtgtgcagcg tttaaagaag aagcactgta
      601 gaattttttc atgtctcgac agacttcctt ataatcctta cttgggaggt atcgtaacgc
      661 atttcgaaca agatgaacaa tacagcgctg cacaatgaca gaaggaaaaa ttgcttttgc
      721 gccttctttg agaccggaaa caccgtccat cgaaatgaaa aaaacatctt ctacaccacc
      781 tactttcagt tcatcaaata cttgcatcca gcgattttta gattctgttt gattgagcca
      841 taggcctaaa atctccttgt ttcctttaag atcatagcca agaatggtgt acacagcata
      901 ttctttagct tcatagtttt cgcgtaaagt gacgtacata caatcaacga atagaaatgc
      961 ataacacttg gctaatggcc gagcttgcca ttcttccaat tcaggaagga cggcatccgt
     1021 gatatcagaa atcatttcgt gggaaatatc aaagccgtaa atatcttcaa cggttgcggc
     1081 gatatcgcat tgactcattc ctcgtgcata catggaaaga accttacctt cgatgtcgga
     1141 gacatctcgt tttctattag gaatcaactc aggttcaaaa gaagcctccc ggtctctggg
     1201 aacatcaatg tttactttac caaagctcgt tttaagcgtc ttattgccat aaccgtttcg
     1261 tcgattgtca tgtttctttg gctctttaga atgggcgtca taccctaaat ggttattcaa
     1321 ttctccttga agcatttttt caaataaggg accaaacaca tcttttaaaa catcttgcat
     1381 atcctctaca gattcgggtt gataagtatt caaaatggat tcagctaact tctcagcatc
     1441 aggatttctt ttctttctag ccatcgtgta atcacctctt gatcttattt tagaaaaata
     1501 aaaaccgtga agcaaccacc cgcctaggat tgatggttac ttacacggtt tatattacac
     1561 tctcattttt ggtgctaaaa agcaccgaaa attttaatta ttgtcaatta ataattcttt
     1621 tggattcctt cttaatatat tgaatgaaga aataatcaat gatacaagaa gtactaaact
     1681 cataaatata actacataaa tcatgaattt aggtaataca ttgatatcta aacttgataa
     1741 tgtcttgtta aatccttcag cttctgcccc accacctaat tggctagatg cagattgttt
     1801 agctatttgt ttagctatat caccagtaac cttattcaat atattattcc caagcttgtc
     1861 agctgtatat tgagctaaga aataggaacc aacgaatgcg gggattgata taaataccat
     1921 ctcaattagg aattgaccaa aaatttctaa ttttgatata cctaatgata gtaatactgc
     1981 tatttctttc tttctagcat tcatccataa gaataataat aatgaaacta caactcctgc
     2041 aaatattaat gagccaaaaa ataatttatt tgaaattgag taaatacctg atatagattg
     2101 ttgtaatgca gggtaatttg atgaactttt aatcaaatta tattctctcc aatttatatc
     2161 taatttccca agatctttta ttacactatc aagattctta tcacctttta caaagaatgt
     2221 tgcatcttgg tatacagctg tatcttcggt attaccataa actttagcag ctgtatgaat
     2281 atcagtgatt aatgtatttt cgtatagttc ttgtgctgca cttactccac cgctattatg
     2341 cccatcgaat agacctttaa tttctacttc tactgtttca tctgcacctt tctcattatc
     2401 agcatcaaat aaattagatt ttatttttat cttatctcca actttaaggt tatttttttc
     2461 agctaaatct ttatgcatta agattttatt tttatcttca ttttctaaat gctttccttc
     2521 tactaattta tatgcttctg atacaaattt tgtttctttt gttgagtcgt taaccccagt
     2581 taacataact gttcttttaa aattctttgc tctttcaggt gattgattcg caagagtctc
     2641 ttgagtttca ataatatcat aatcaactaa atctgcaaca ctgtttattc tttttacata
     2701 ggagtctata ctatctgttt gagatatctt tttaatatct tcacctttta catttccccc
     2761 acctctaggt gttcctggat ttacttgtct atttatctcc atagaaaaac tatttgttat
     2821 attagcaaat gtttcttttg aagctctgtc cgtagcatct ttaatagata aactaatgag
     2881 acttaaagtt gacatagata aaataactaa tatgataatt aatgatttta aactttttct
     2941 agttacataa gcaaatgcat tttttatcat tattcaacct ccaaattcat tttatttact
     3001 tttttcaact ttttaccact tagttctaaa atgatatccg cagaatctgc tacttcctta
     3061 ctatgtgtta caactattac acatttattt ctatctttag ctaatgtctt taatatatta
     3121 attatttctc cagcagtaac actgtctaga ttaccagtag gctcatcagc tagtattatt
     3181 ggagcatctg ataccaatgc tctagcaata gctactcttt gttgctgtcc accagataat
     3241 ttcataacat ttctttttat ttgtttttta tctaaaccta gttcaaacaa gatactttca
     3301 tctgctgatt tatttactaa tctaatattt tcaattggtg ataaataatc tattaaatta
     3361 tagttttgaa atactaaaga tatattattt tttctatgat tactatatcc tttattttgt
     3421 atatcttcat tcttaaacaa tatttttcct gtttgaggtt tatcaagtcc agcaagtaag
     3481 gaaagaagtg tagattttcc tgttcctgac tttcctacta tcgcataaaa cttcccaagt
     3541 tcaaattttt gatttactcc tgacaaaact ttttctttag aatttgcata actatatgct
     3601 acattcttta tttctaatat atccattatt ttctcctaac ttatttttga taagatttct
     3661 ttaggcttct tgattaacat taatgaagaa gcaaatacaa ctgataaaac aataatactt
     3721 attaatatta aataactttg tccaagtgtt gttatatttg acataaaact actattattt
     3781 attaaacttc caccggaaat cattgaatcc tctgagttaa tgaatccatc tacaattact
     3841 tttagtaata catttcctaa aaataaggaa gatattatac ttggtatcga tatgaatatt
     3901 aactcgaata taaattgcat tataatttgt atcttagatg ttccaataga taagaatata
     3961 cctatttcat aaattctttc ccttaaccat agaatcaaga ttaatgaa
//
LOCUS       DACDCV010000155         3978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDCV010000155 DACDCV010000000
VERSION     DACDCV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.158"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..278)
                     /gene="mobC"
                     /locus_tag="IV041_002731"
     CDS             complement(<1..278)
                     /gene="mobC"
                     /locus_tag="IV041_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP8383462.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWC"
     gene            complement(728..1015)
                     /locus_tag="IV041_002732"
     CDS             complement(728..1015)
                     /locus_tag="IV041_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8383463.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(1035..1259)
                     /locus_tag="IV041_002733"
     CDS             complement(1035..1259)
                     /locus_tag="IV041_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8383464.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1899..2432)
                     /locus_tag="IV041_002734"
     CDS             complement(1899..2432)
                     /locus_tag="IV041_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8383465.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2453..3397)
                     /locus_tag="IV041_002735"
     CDS             complement(2453..3397)
                     /locus_tag="IV041_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8383466.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1110 a          751 c          628 g         1489 t
ORIGIN      
        1 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
       61 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
      121 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
      181 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
      241 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
      301 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
      361 ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac ctacgtattt
      421 ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctccccccca
      481 ctctccacaa tacaaaaaag actagcaaat gctagccact ttttgtaaag tttcgcttgt
      541 ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt tttttcgttg
      601 ttcaaagatt cttatgccat ttactattaa taaatctata aatatgataa tttttcggct
      661 ctataattta tgggactgat gaatcagaat tgattcttca gtcccatttt cattttattc
      721 caaagaatta actccaaata ccaatagaag cccatcctct atatacttga gattccttac
      781 taacaatttc ttgtaattct attaaagatt taggtaatcc aaatttatgt tttaaaatat
      841 atcttccaag aaaactactt aatttcatac taacttcacc aacacttttt ccctcactga
      901 tttctttaga ataaagaaag agcttttctt ttaagtcagg atagtcatct atagttgctt
      961 cattgtatac tttactaatt gcatttaaaa gtttttcttc tttagtaaaa tccattataa
     1021 taacctccct accactacct tctaggtgcc catggaccat gattaaccca accattaaca
     1081 ataatttttc caatttctcc tttagcttga ttccaatcta cccaacattt ttctttgtta
     1141 caataaagac catttccata gtaagtagct gcatcaactt ttattcctgt accgatgcca
     1201 gctagacaag ttcctaatat tcctagaata acacaatgtt ttaatacttt ctttttcatg
     1261 attgtttttc cttttcgttt ttttaaaatt gcttgttata ttaaataatc ttagtaattt
     1321 tattatataa cattttatta tataacaatg attaacgtca taaaaagtag ttttgaattc
     1381 ataatcgttt aaataaaatt ataagatcag ttatttaaac agctagtaga cataatgtgt
     1441 atactaccaa gtaacgcctg taccaaggga tccaggcgct gattttgtga atttttattg
     1501 gtctaggtta aaagctcttt ttaaggcttg gttttctgcg gttgtttctt tctaattctt
     1561 tgtgaatatt gatcggtcat tttttaaata cttgttattt ttgtttgtgt ttgttatgct
     1621 ttttacatat ctcatattca tttattattc ttttctaagg ttaataatag gataatctct
     1681 ttcagaaaga ctgctcgtac gagcagtctt ttcttattta tcaagttttt ctacaaatac
     1741 ttcctcattt tgactgttgt agtaattagg attgagtttt agttcccctt ttttatttcc
     1801 tttgttggcc actgataata acattgggct aaagcttaat attcctagta ggatagaacc
     1861 gaatagcaag tgtttttttg tcataagtat ttccttcttc atttccatag ctgccaccat
     1921 tttttccaat tctttgtttg ttcgtccatt gtttctgttg cttcagaaag agtcgttgaa
     1981 ctattctctg aagactcttc cgtataagtc aactgcagtt gctcctgaag tttttcgatt
     2041 tgttggttgg cttgcagggt gagttgttgt tgctggtcga gtagtttttg tatatgagaa
     2101 ttattttgct ctttgagggc aagttgttct cgtaaaaatt cattttcttc acgcaactga
     2161 tctagcaact tgtttgcaac cacgtttttg tttggtatat aagcattttt atcgttgttt
     2221 tgttcatgtt tatgcaactt atctagcaac ctttgattgt ctaagttgcg agcaagttgc
     2281 aatactccag aacgcaatac aaccttataa ccacgctcat ttttagctat catggaagaa
     2341 tctaattttt ggatatgttt cgatattgct tgcctactca tcttaaaaag atctgacaat
     2401 tcctttattg taaaggtttc ttgtggttgc atactggttg cctcctaggt tgctactgga
     2461 ataaattatc taaatcagat aataaacttt ttttctcttt ttccgaaagt gcggactctg
     2521 tttgtttttc aagagctttt ttctcttttt ctagttgttt gatttgttgg aggagttcaa
     2581 gttctcgttt cttctgtttg attttttcgt taattgttgt agcgggttct tcgtattctt
     2641 tttgtttatc agctaaagcc aatgctttat tttggtcttc tttggttggt gcatgtttgt
     2701 taaagtcgtc tgcttttttg atctcaggtt tgaacgtaaa ttgatagcca ataacaggtt
     2761 ttccccgtcc ttttccgtat ttctttttga ttgttagtcc tttgaagatt gcagttagtt
     2821 cttgacgtat aggttttata actcgtttat ctatatcagg ttgcttataa cttttaggaa
     2881 tatctaataa ttcaagaaag tcttctttgg agaaatatgc gaaacctttg gttctgtatt
     2941 gctttaacag acgaaacata gtctttgcat agccactttg tagctcgttg aattgttgta
     3001 aagaatagcg tacccactcg tctaaattat ttaacaaagg cagtgctttt tcgtaaattc
     3061 gtatatctac gtaaggattt tctacgtccc cgttgatttt aaattctgta aacattacaa
     3121 acatatcgta agaaagccca gactcacttc tacgaccaaa gcgtaaagat aagatttttt
     3181 cataagttgc ttttaaatct tctacaaatc gtacatttgc agttgctttg tagttactta
     3241 aatcttttag ttggtcaaac gtaaaacgaa ctgtcttatc ccctttttca cgcattctag
     3301 acacgatgga aaaaaacaaa ttcatttcaa ccggtgtaaa ttttcttaaa ggaattgtat
     3361 ttaattcatg gtggtatttt acaatctcat tagccatata atcacctcaa tccaattatg
     3421 aatgacattt tttaaatgtc aagacattca tatttgacgt tcaaacagac aaaatgtcct
     3481 tcataaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa acagacaaaa
     3541 tgtccttcat aacattctgt atccattggt agagtaagga cacagcttac ggtaaagagg
     3601 tttaaaaaga ggtttaaaaa gagatataaa aagagggggg ctaattttcc cgcctaaaaa
     3661 ccacttcttt tttctttaaa caggttcgcg catttttcaa aactgccaat ccaaaacctt
     3721 gcttcctacg gtcgccaaaa aggtgtgatc gctacgctct cacattgtac tgcgcagtcg
     3781 cctaaaggct cctttgctaa aaacatgttg gtaaagaggc ctttgagcgg ttctgaatgc
     3841 ttatctaagc cgttttaaga cagtcttgga taaaaatcct tatctagctt taaagcgcct
     3901 tacaggcggt cagggcaagc cctaacaacc ctaaaataag cgatacttaa aaacaccaca
     3961 caagtttaat tttttaag
//
LOCUS       DACDCV010000156         3754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDCV010000156 DACDCV010000000
VERSION     DACDCV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.159"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IV041_002736"
     CDS             127..579
                     /locus_tag="IV041_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383467.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IV041_002737"
     CDS             593..1282
                     /locus_tag="IV041_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383468.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3754
                     /locus_tag="IV041_002738"
     CDS             1407..>3754
                     /locus_tag="IV041_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8383469.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKL"
BASE COUNT         1274 a          492 c          775 g         1213 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaa
//
LOCUS       DACDCV010000157         3656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDCV010000157 DACDCV010000000
VERSION     DACDCV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.161"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(254..1228)
                     /gene="sgrA"
                     /locus_tag="IV041_002739"
     CDS             complement(254..1228)
                     /gene="sgrA"
                     /locus_tag="IV041_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8383470.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1424..2263)
                     /locus_tag="IV041_002740"
     CDS             complement(1424..2263)
                     /locus_tag="IV041_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8383471.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2448..3335
                     /locus_tag="IV041_002741"
     CDS             2448..3335
                     /locus_tag="IV041_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8383472.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1226 a          745 c          542 g         1143 t
ORIGIN      
        1 ctacatgaat aacgtacaaa tagattcgtt actgattttt cagagatact accatgcgca
       61 gtgtgcattg tcaattattc ttgctcttat tttccatttg tgttcctgtt atttcctgtt
      121 tctcaaatat gttagtaaac aaaaatagat ctggcaaagc tacaacttcg ccaggcaata
      181 cagcactctc gaatgaaata ttcactcaaa aatctatcgt tttattcaat tttagatctg
      241 ttgcttttat tctttatttc actcgatgga agagagaacg aaaatagctt gctcctacaa
      301 tcaaaatgag taacacaccc atccatgtaa ttagcttgtt caaggttcta ctaccagttt
      361 ctggtaaatc gctttttcta ttatctgttt ctttcgcttt ttcttgatga tcaattccac
      421 gagaaacaaa atcaggctgt tttagcacac tttttaggtt gctccctgca tttctctttt
      481 gttccttagt tggtatgata ggtaccaaat caagttcatt atttactttt cctgtgctgc
      541 tttcgctgct cgtgcttcct gtgctactct cactactcat gcttcctgtg ctactttcac
      601 tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg
      661 tactctccgt actgctttcg ctgctcgtgt tttctgtgct actttcactg ctcgtactct
      721 ctgtactgct ttcaccgctc gtgttttctg tactgctttc actacttgtg ctatccgtac
      781 tgcttggcgg tccaacaact gtccaactgc ttggtttcaa attctccgtg atcgtcatct
      841 gtccatcata aaaaacaatc aaatcaactt tatctttact cttgatctca tcaatcgata
      901 gttctgcaga aatcattatt gtatactcaa taccagcggt ccattgtacg tcttttaaat
      961 aaacttcccc aataactttt tctgaacttc cattaccatc ccaaccatat gttagaacag
     1021 catgtacatc actagcattc acgcttttgt ttggtttgac tggaatagta atgacttgtt
     1081 gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa tgaaagattc tcatttgccc
     1141 gttcatttgc agaagtactg attccaatca ttgctgcaat tatactcagc ccaagaataa
     1201 caaataatac ggtcgctgtt ttcttcattt ctatcactct ccatataatt accttctatt
     1261 tgataaagga attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct
     1321 aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga
     1381 cttaaaaaag caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag
     1441 tgtatttctt catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta
     1501 acaagaataa tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg
     1561 tattttgcga ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta
     1621 tacccaaaat agctccttgt gtccaattcc aagtaatatg agcaccaata gccgtaaata
     1681 aacttcctgt actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa
     1741 ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc
     1801 caatcattgc tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata
     1861 aaagctcttc tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa
     1921 tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa
     1981 gcagaaacac tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc
     2041 gaacagaaac accataatct tctattcttt ttccttcgac cttactagca agaaataaag
     2101 ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat
     2161 ttccaataaa catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacta
     2221 agactattaa aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta
     2281 tattaagttt aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat
     2341 catagagaga atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa
     2401 gactgtctcc ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt
     2461 tgaaaaaaca actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt
     2521 gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt
     2581 tttatgataa cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct
     2641 tcaaagcatt tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat
     2701 ataatgaaca aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc
     2761 aaacagaaga gacatataag aaactcttta aacagatgct ggctttccaa tacggtttga
     2821 aagccaatgt aaaattaaca gataaagatc tagacactgc ttggaaagaa ttctatccag
     2881 aagttagtac acagatcatt ctattttcga ctaaagaaga agcagataaa gcaaaaaaag
     2941 aagcaaatga aggggaaaat ttctccaagc ttgttcaagc ttatggaaaa aataaaacat
     3001 taaaggaaac tgatggaaaa atgaattttg attcaaccaa tccagagatt cctactgaag
     3061 taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag
     3121 atccaacgac ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa caggataaag
     3181 gttctaataa agacaaatac aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc
     3241 ttgatacgga attcatggat aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta
     3301 ttaaagatcc ttatgtaaaa aatatattca aataaacaaa tttgacccaa ttggggatgt
     3361 ggcaaaattt gagaagtgat ttttggaaat tccttcttat ttttgaggca aaccactttt
     3421 gccacaactt catcggggcc tttttttatt tgtaccaaaa caaatcactg gatttgttac
     3481 aatcatgagg atataattta aaaaagtttt ttatttctat cacatgaatc aatgcgccat
     3541 taagtagatt ctttcatgct tccctatttt ttcacttgcc gtatcgcttc aaaaacaact
     3601 atcataaact caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat atccct
//
LOCUS       DACDCV010000158         3630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDCV010000158 DACDCV010000000
VERSION     DACDCV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.162"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..591)
                     /locus_tag="IV041_002742"
     CDS             complement(<1..591)
                     /locus_tag="IV041_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8383473.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVET"
     gene            complement(584..925)
                     /locus_tag="IV041_002743"
     CDS             complement(584..925)
                     /locus_tag="IV041_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383474.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(1099..1824)
                     /locus_tag="IV041_002744"
     CDS             complement(1099..1824)
                     /locus_tag="IV041_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8383475.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1877..2353
                     /locus_tag="IV041_002745"
     CDS             1877..2353
                     /locus_tag="IV041_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383476.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2359..2541)
                     /locus_tag="IV041_002746"
     CDS             complement(2359..2541)
                     /locus_tag="IV041_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383477.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2541..2840)
                     /locus_tag="IV041_002747"
     CDS             complement(2541..2840)
                     /locus_tag="IV041_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383478.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2945..3148)
                     /locus_tag="IV041_002748"
     CDS             complement(2945..3148)
                     /locus_tag="IV041_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383479.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(3163..>3630)
                     /locus_tag="IV041_002749"
     CDS             complement(3163..>3630)
                     /locus_tag="IV041_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8383480.1"
                     /translation="SEVLPTIRKTGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEY
                     EPKISYLDTILSSTDTVATSQIAADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMN
                     QGYTKSHTSEIPKADGGTKVVMNTKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          987 a          726 c          559 g         1358 t
ORIGIN      
        1 agtttcaacg ttatcagcta agagccctaa tttatctgtc tgctcattaa catattcgat
       61 taaggttttg gtcatatctt tacccatctg ctcattgaaa gcttctagaa tcgtctctag
      121 catgtttaat ttctttgtat cgattctagg tggtgttgga atatcttccc cttgaaatac
      181 atataatccc agtccgtgta gagccaatgc tttcacaaag catcgcttca atgagttatt
      241 gatttgcatt gcatttggtt taacaactgg ttggtttcga taatctaaaa caggaaataa
      301 ctctgtttcc gtatgtcctt taaccgttac tgagacagat acataagtcc ctgtttcatc
      361 cataagaaaa ggtttatatt cctcaacaag aaagtcttga tgagttccag aaacaaccct
      421 gtagtgttta tactcattaa tagttaccgt tgcctgtgga tcattctttt tcataatctc
      481 ccacgcgtga gcccaagata aataatcaaa atttcctttt ttcttgagaa ttttatttaa
      541 cttacgacta aaaagttttt caaagttcgt tgtccctttg atttcactca tcaaattctg
      601 cctccatttc agcaatgtat ttcttacctg atccgtaata agagatatca atcaagttat
      661 ctctgttgta ctcttctagc gcatcaatca agccatcttc gatgacatag atgtattcag
      721 gtttatttga atgttttgat agatggataa ggtaaacatg atcccaaata ctcacaaaat
      781 ttcccaagtc atcttgatca catgatagtt cttcgttcgt caaaagattt cgtctgattt
      841 ttcgattatt tgtttccttg atattcgact tgccccattc agaatcagtc aaatattgat
      901 ctaaagtgga aagttcttta ttcatatgct aaaattctcc ttagatatgt tttgtgactc
      961 tatgcttgcc ggctgagtca cttttttatt tgttgccatg ctttttgctt ttcgatatgt
     1021 tgttggctta ggatgtttgg tttattgtgt ctccaccagc gattagcaat taccgtccct
     1081 attcttagcg cttcagctct attcattttc atcaccgaaa agtctttgtt gtctgttcag
     1141 ttgatcgatt tccatgcgga tcgcagtttc tggtaaccac atttcaataa atgaaacagc
     1201 atcatcgaat ctcttacgag gtaactcgcc atatcttggg attgaaaagg tacgtttaaa
     1261 ttcagaccaa aattttgaga atactttttt gctgatttct tcataagctc ggctttcttt
     1321 tccccctaga acttccataa ctttcatatt tcctttttgc ttaatttcaa actcttgttg
     1381 tccgctaatt cgcatagtat ctttaagcat ggaaacatct tttttaacat ctttcatttc
     1441 ttctaattga tagatcatca tgtcttcaat tgtttgagga acagtattct tccgaataac
     1501 atcttccatt tcgttgaaag cttcaataaa ttttagtttg aaatttattg ctttacttcc
     1561 agtgaatccc atagctagca aggaaaatcc gtctctattc atgaaataaa ctctccgact
     1621 tcttccgtat gagtctggct cgttcccttc cacaaacatc tgtccaaaat tggacccatc
     1681 ttcaacattg gccgaatttt cgaccgatct ttttattgat tcaattgctt ctagtacatg
     1741 cttatgtttc tttttgaaac tttctgccac ttgtaagctc gtagttacag cttctttatt
     1801 tttcaaaatt actaattctt gcattatttc ttctctcctt tttggtataa ttttcgttag
     1861 aaagtgaggt gattctatga ggaaaaagct caattacatt cttactgcga ctttaaccat
     1921 atatataatt ctgtttatta ccttatcttt cctgtcttca attaaaaaaa ttccttttat
     1981 tacattgaat aaaaatagta ttttgaagga gttcaataac actgactaca tcgcatttct
     2041 aaaacttttt tcagtctgtg tgctaatatc atgtgtaatt atttttattg ttgctaaaac
     2101 atgttggaca atgttaaata aaaaccaacg cgatgttgaa aatattgcta tatttttaat
     2161 taagattact tcctcaatat caattatcat tccgatgact gtaacttttt caaaagaaca
     2221 gtttaatgta gcggctactt atctttcatt tatagcgcta ttttcttttg ttcttcctaa
     2281 aagaaataag agcaactttg acaacaagga gctggatgaa gtacgcaata gcagcccaaa
     2341 ggaacaaaaa taaaaaaatc atattttagt cagtccctcc cgactggctt ttttcgctct
     2401 gtgctcagct tcatcaagcc ccataaaaat ccaaaccatg taaacgattg tccctatcaa
     2461 cgcttgtctg cttccccaga ggcctaaagc gtagatgatt agcggtgcgc tgaatactag
     2521 tgctctgttg aatttgccca ttaacttgcc tccttcaaaa catctgaagg cgataaactt
     2581 tttacacgat atttgtttgc gtctttccac tttaaaaacc aaataaaagt atggtaatga
     2641 ataaatgtgg ttgagtgacc tggacgaagt attccttctg caaattcagg aattgtttcc
     2701 atttccttgc agtattcaac taaagtagtt tttgacatgc cgtggaactt ctccaagatt
     2761 aaactttgtc ggtaccagtc gtctggtttt actgccttct cagcagcttc tatcagctct
     2821 gaaagtgttg gttttttcac ttattatccc tcctaaaaat tatcgttcca tactcgatgt
     2881 ttaacatttc ttgaagctct gattttgtaa gaatgatttt atcttccaac gactttacct
     2941 ccctctacaa ttcgtacata gtgataatcg aatctataat tctgtttgct tctgcagaag
     3001 tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat ctttcagcaa
     3061 gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt tctctatctc
     3121 gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa tttcctctaa
     3181 atccatttga gggtaatagc cctctgcgat taataagttg taaataaaca ctcgcccttt
     3241 ctgtgtccat ttggtattca ttacaacttt agtgccgcca tcggctttcg gtatctcact
     3301 tgtgtgcgat tttgtgtatc cttggttcat atgttttcgg taaagtatcc attgtccgct
     3361 aactttatgc tgaacaccta actcgtttag caatttgttt agagcaattg ccgacattcc
     3421 gtaatcagct gcaatttgag aagtagctac cgtatctgtc gatgaaagaa tcgtatctaa
     3481 gtagctaatc tttggttcgt actcggcaat ttgttgttcg agtaattggt tcttttcttc
     3541 taaatccgct gctaaacgca atgcttgtgc aaaactttga ggtacgtttg aatagctacc
     3601 tgtttttcta attgttggaa ggacttcact
//
LOCUS       DACDCV010000159         3489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDCV010000159 DACDCV010000000
VERSION     DACDCV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.163"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..887
                     /locus_tag="IV041_002750"
     CDS             69..887
                     /locus_tag="IV041_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8383481.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1117..1347
                     /locus_tag="IV041_002751"
     CDS             1117..1347
                     /locus_tag="IV041_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8383482.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1570..1668)
                     /locus_tag="IV041_002752"
                     /pseudo
     CDS             complement(1570..1668)
                     /locus_tag="IV041_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1933..2388)
                     /gene="tnpA"
                     /locus_tag="IV041_002753"
     CDS             complement(1933..2388)
                     /gene="tnpA"
                     /locus_tag="IV041_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8383483.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2631..3489
                     /locus_tag="IV041_002754"
                     /pseudo
     CDS             2631..3489
                     /locus_tag="IV041_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1154 a          586 c          612 g         1137 t
ORIGIN      
        1 gtatttagga gtttttttta ttattttgga gtataatgga gaaaagttga aattgtagag
       61 gagtttgtat gagttatatc tatttaatta aagaaaatta tccattttta acgaagtctg
      121 aaaaaaaagt cgctgatttt attttaaatt ctgggaaaac tattatttat agtacgatga
      181 gtgatattaa gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa
      241 ttggtttttc tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa
      301 aaaaagaaca tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag
      361 ctctctattc cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga
      421 tcactcaatc aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg
      481 atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt
      541 ttcaagccca agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat
      601 ctgggaaaac gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa
      661 ttattgctat aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac
      721 aaacagccat tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt
      781 atatttgtga tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat
      841 taagagaaaa agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt
      901 agctaaacct aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt
      961 gatagcttat tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct
     1021 acggttcttg attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag
     1081 aaaaacgtgt agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct
     1141 ctcttaaata aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag
     1201 gaacctactg aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg
     1261 ttttctataa agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt
     1321 gtttataaga acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact
     1381 tcaatgttat gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta
     1441 aaagtttatg atccttttga tttttatatt aatttatctg tatttaatac agattttatt
     1501 tattagttca tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac
     1561 tcttaattgt caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc
     1621 caaaggtgtt cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct
     1681 aatgtttgaa tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa
     1741 attgccaaaa tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt
     1801 ctatcagtaa cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc
     1861 gacactcgct gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc
     1921 acttacttct ctttattttt taaatggttc tacgtactct cttttactta taatatatcg
     1981 aatgcgatca tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc
     2041 gactgtactc acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag
     2101 atttgcgtgc tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact
     2161 ggatatacat atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg
     2221 tgcttctatg atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc
     2281 ttttcgtaat tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata
     2341 cttacaattc caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct
     2401 ttcgatctga tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct
     2461 aaagatactt cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac
     2521 aaaactgact gaagtcatta agaaaataaa agggtctaaa acttttaagt tttgtaccga
     2581 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtgtttttt atggctaagt
     2641 atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa ggcggaagtg
     2701 attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc tggatcaatg
     2761 cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa aaatattctg
     2821 ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc tatcgtgaag
     2881 ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg cgtcaattcc
     2941 gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga
     3001 aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac
     3061 gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag
     3121 aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa tcgcacgaat
     3181 catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt taaagttccc
     3241 taaggcaatc tttatgtact ggcaaaaacg attggaccgt aaaaatccag atcaagagat
     3301 tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt tacggatgac
     3361 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcctgatcaa
     3421 gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca attcttatcg
     3481 aggacaagt
//
LOCUS       DACDCV010000160         3423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDCV010000160 DACDCV010000000
VERSION     DACDCV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.164"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV041_002755"
     CDS             complement(109..564)
                     /locus_tag="IV041_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383484.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV041_002756"
     CDS             complement(718..1926)
                     /locus_tag="IV041_002756"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8383485.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV041_002757"
     CDS             complement(2038..2370)
                     /locus_tag="IV041_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8383486.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV041_002758"
     CDS             2518..3393
                     /locus_tag="IV041_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8383487.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT          944 a          709 c          610 g         1160 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaa
//
LOCUS       DACDCV010000161         3396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDCV010000161 DACDCV010000000
VERSION     DACDCV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.165"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..642
                     /locus_tag="IV041_002759"
     CDS             196..642
                     /locus_tag="IV041_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383488.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            644..781
                     /locus_tag="IV041_002760"
     CDS             644..781
                     /locus_tag="IV041_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8383489.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            819..1112
                     /locus_tag="IV041_002761"
     CDS             819..1112
                     /locus_tag="IV041_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383490.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1109..1333
                     /locus_tag="IV041_002762"
     CDS             1109..1333
                     /locus_tag="IV041_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8383491.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1330..2355
                     /locus_tag="IV041_002763"
     CDS             1330..2355
                     /locus_tag="IV041_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8383492.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            2545..2618
                     /locus_tag="IV041_002764"
     tRNA            2545..2618
                     /locus_tag="IV041_002764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:Ile,seq:cat)
     gene            3295..>3396
                     /locus_tag="IV041_002765"
     CDS             3295..>3396
                     /locus_tag="IV041_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383493.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREY"
BASE COUNT         1172 a          566 c          672 g          986 t
ORIGIN      
        1 gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc
       61 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
      121 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
      181 ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca
      241 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa
      301 ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg
      361 aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca
      421 atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg
      481 ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta
      541 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
      601 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
      661 tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
      721 gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga gcgcatctta
      781 attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta ggggaatggg
      841 gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta ataaaaccaa
      901 ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac aatttagatt
      961 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
     1021 agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac acgcaacaaa
     1081 ttaaaacttt atttagggag agaagaaaat gaataataaa acgttcgaag tactaaaatg
     1141 gttcgcactg gtaattattc ccgcactagc tactttcgtg gggttagttg gtaaagcgct
     1201 caattggcag tacacagata tctgtgttgt catcattact ggttttggcg cgtttttagg
     1261 gagtgtgttg ggtgtatcaa atcgaaccta caaaatgttc tcggctgaaa gcgaagaagg
     1321 aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcctgttaac ggctcttttt
     1381 ttattaccca ttaatacgtt tgcttatact attaatgacg agtataattt agcgccgaat
     1441 caaggagact ccagattagc aattcctaac aaaattattt tgcacgaaac tggaatagat
     1501 gcaccagcaa gaaacgtagc cgccaacatg aaaaataatt ataacggaag caattcttat
     1561 actacagatg ttattggtga cggtgggatt gtttaccgtg tgggtgagca aggatatgtt
     1621 tcgtggggag ctggtaacgc taatccttat gcgcctgtac agattgaatt acagcgcaca
     1681 tatgataaag cattgtttga aaaaaactat cgagcttata ttgaatatac aagagatagt
     1741 gcaaaaaaat atggaattcc attgactctt gatcaaggaa cttctttatt tacaaaagga
     1801 atcatttctc atttgtgggt gacaaattat gtttggggga accacacaga tccatatggt
     1861 tacttatcgc aaatgggagt tagcaaagaa aagcttgctt atgatttagc tcatggattt
     1921 accgatgaaa atccaacaac ttctgaaaac aagcctgtca ttgatccaac acgagctggt
     1981 gcagctaatc ctacgctgac agatggaaca aatcacgccc acattgatca gtttggggaa
     2041 atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa atacgagtat
     2101 attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt
     2161 tatagaccag atgtaaacca agcttataat acttctggaa atgttggtta tcatgtatct
     2221 ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa tgatgcgggc aacgaatgat
     2281 ccagagggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat
     2341 attcctaaac gataaaaata gctcctcgtt gaggagcagt acataactat attgacaact
     2401 ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccataaata
     2461 gtcacactcc aagctatgcg ataacaggtt tgttgccaca cattctactg gttgattgtt
     2521 tatggcttta tgtggcaaca accagtaccc ttagctcagt tggtcagagc agacggctca
     2581 taaccgtccg gtcgtaggtt cgagtcctac agggtacatt aacgtagcca tttgaatcgt
     2641 tctgtgttag aatttttttg aagagtatta tacaagctaa agcttttctt cattgccact
     2701 caaatgagtg gcttttttat gtatcctttt atggattaat gaaaggatgt ttcacatagt
     2761 tatacttctg tatatttgaa aagttttact ttgattttta aatagaaaga catttgggtt
     2821 aaattgtgag ataataataa agaagagttt aaagcgcacc ccaaaccact tccccataag
     2881 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
     2941 aaaaaagtga gaaaaaaggc ttacaattgg agtggtagtt aattagtgac ttatttttga
     3001 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
     3061 cttaaaaaat atctcctttc acaagtatgg tgataaaatt cgttccgggc taccttttta
     3121 ggtagcctac tttaatcttt atacctttct ggatcaacga aagtatactt tatatagtca
     3181 taacgccgat gatcgctacg tgcgtccggc acgtcagtca tgaatcggct acactagact
     3241 agacagaaaa aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca
     3301 agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcga tctctatctc
     3361 gctggtaagc ctcgcgcaga aattattcga gagtat
//
LOCUS       DACDCV010000162         3343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDCV010000162 DACDCV010000000
VERSION     DACDCV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.166"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..487
                     /locus_tag="IV041_002766"
     CDS             <1..487
                     /locus_tag="IV041_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383494.1"
                     /translation="EFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
     gene            complement(558..1868)
                     /locus_tag="IV041_002767"
     CDS             complement(558..1868)
                     /locus_tag="IV041_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8383495.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1880..3298)
                     /locus_tag="IV041_002768"
     CDS             complement(1880..3298)
                     /locus_tag="IV041_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8383496.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1061 a          689 c          528 g         1065 t
ORIGIN      
        1 tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa gtgccgtcat
       61 tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg gccgtaaggc
      121 attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa acttctttaa
      181 atctattacg tttgactgtg gaaaagaatt ttctaactgg aaagccatta gtaaccaaca
      241 tgatattgat atatattttg cggaccctgg aacaccttct caacgcccat taaacgagaa
      301 ttctaacggg attctgcgtc gtaatggact gccgaaatca atggatttta gaaaagtgaa
      361 tcagacattt atttccagtg tcagcaatca acgtaatcat attccaagaa aatcattgaa
      421 ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt attctagctt
      481 aatttgacaa atcatatatc attaaaaaaa ataaaggtca agctaaaata ttatttaagc
      541 ttgaccttat tataatttca acgattgtca aaagtattgt ttgcagctgt gttgccagtt
      601 tctatagata aatcacagct tttgaaacat aagttttgta ctgcttcgat tcgcccgaat
      661 tcttttgcag ttatttggca atgagaaaag cagacatctt ctataggctt ttcaggaaat
      721 gctactaaat caaaagctcg ggaaggaagt gagtaacctg gagaaaggca tgcctgtacg
      781 tttttgatgt gtatgtcttt gactttcgtc atttgctttg attcaggtat ttcttcagcc
      841 actgcttgcc aactttctgg catttgagca aggtcattgc tattttttct attgtattca
      901 ttatgccagt ccattatcca agaaaaagga aattgtacat caatcatatt cagattttct
      961 accctgatat tttcgatcac tccccctctt tcttttgaag atttcattcg aataccacag
     1021 tctgactgaa aaaaattgat atcgtgaata tatacgtcac taactccagc actaacttcg
     1081 ctaccaatag ttacaccgta tcctgaacgg attttacatc tagcaacctc gattcttgat
     1141 gattttcgat tgactcttgc accattgcca tctctgccag acttaatagc aatacaatca
     1201 tcaccacagg ccaaatcaca ttcatagaca cgcacatttg ttgaagaatc aatatcaatc
     1261 ccatctgtac ttggtccatc attgttgcgg atgattactt tttctactaa tacatcgtta
     1321 gaataagtga tttgcaagtt ccaaaaacct gatttctgaa aaatcaaatc acgaacaaca
     1381 acatgttctg catgatacag taaacaagcg cgtggacgtt taatagcata atccgcaatc
     1441 caacgaagtc ccttttgatc gtattcagct cttgtcccgc ttttttgatc ttttccccaa
     1501 tacagctccc accaatggtc tccttgaccg tcgattattc cttttccttc tataagaata
     1561 tctttcgccg agagaacatt caaaatcgct gctggccatt tcatttctac gccggctact
     1621 cgtgtatcga tttctggaaa tgcttcgata tctgttctcc ccttgaggac tgcaccttct
     1681 tcaaagataa gatgactctt atcttttaaa aaaagggctc cgactaaata aatgcctttt
     1741 ggaataaata caggtaagtg ttctttcgca ccttgatcga ttgctttttg cagtattatt
     1801 gtatcatctt gttgagtgtc gtttgtttta aagttcaaag cattgataat cccttttttt
     1861 gtccgcaata cgtttctccc tatctttcat tgattttttt caattcttca ccaataaata
     1921 cagtttttga acgattcagt gggtaaccta ctagcaaaat gattgcggat acaaataagc
     1981 agaatgctgg taacaagttt gccatcgtat aaatcctttc ttgtactaaa actgattgga
     2041 tggctccacc tgttgtagac gattggtaac caatgagtgc cagcataaat ccgcctaccg
     2101 ctcctgcaca tgcttgtcct acttttctcg caaaagaatt tactccatat actgttccat
     2161 cttctcttaa gcctgtctta tattgatgat aatcaatcac gtcagtaatg aatgcccaga
     2221 ccatcaaatt gaataaacca gatcctaaag tagcgataaa taaaagaatc agatataccc
     2281 atgcatttgt tatatgaagg aaatacatca gcaagtacat acatgaagaa gcaagtaacg
     2341 caaatacgct tgcttccttt ttaccaaata attttgtcag ctttgtagca aatggtgcta
     2401 aaactactac tgttccgtaa gtaaataaaa gagcaacaga cattgcttgt ttattatgga
     2461 agtaatcatt gaataaataa gtcatattcg tcccagctaa aatctgattg atcacaatga
     2521 aaatgtcaac aatgactaaa ataatcagtg ctcgattagc taacaaacct tttaccaact
     2581 gagaagcaga tactttttcg tttttgacaa ctcttactct ttcagtagtc aatttacaag
     2641 ttaacatata tgcgacaaat gctattgcag cacatccgat tgaaatccaa aagaaatgat
     2701 tccctgaaac gacttgttgt ccactcttgt tatgggtata cattaaaatc ggaatgaaga
     2761 atcctgtagt tgatccgcca atcgcagaac caatgcttcg gaacgtggac aacgaggtgc
     2821 ggtcattagg atttgagcta attgctgctg ccattgctcc atacgggata ttgattgttg
     2881 agagaaaaat cccataaaca atgtatgaaa caaaaatata agcaatcttc accgtcattg
     2941 gaagatgttg gacgaatggc aaaaacaaaa tgacagtcac taagcaaaat ggatacttca
     3001 tttttctgat ccaaggaata aatcggccag tttctgttaa ctctgagaca tcacataatc
     3061 ttccaaccat cacatcagca aacgcatcca aaattctcga aatcaagaat aacgtaccta
     3121 cgattgcccc tgaaacgcca agaacattcg tataataaat catcaagaaa ctattgacta
     3181 aacctaaaat aaaacagtta cctagatcac cgaacatgta gcctatctta tcggaaatcc
     3241 caaattcctt tttttctttt tttactgtat ttgctgtaac agcattttgt gaaagcattt
     3301 gaattcctcc tacctcttct agtaaacgtt ttcaaaatta taa
//
LOCUS       DACDCV010000163         3313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDCV010000163 DACDCV010000000
VERSION     DACDCV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.167"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(530..1144)
                     /locus_tag="IV041_002769"
     CDS             complement(530..1144)
                     /locus_tag="IV041_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383497.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1750..2127)
                     /locus_tag="IV041_002770"
     CDS             complement(1750..2127)
                     /locus_tag="IV041_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383498.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2427..3308)
                     /gene="galU"
                     /locus_tag="IV041_002771"
     CDS             complement(2427..3308)
                     /gene="galU"
                     /locus_tag="IV041_002771"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8383499.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1015 a          673 c          503 g         1122 t
ORIGIN      
        1 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
       61 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
      121 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
      181 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
      241 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
      301 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
      361 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
      421 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
      481 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
      541 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
      601 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
      661 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
      721 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
      781 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
      841 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
      901 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
      961 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
     1021 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
     1081 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
     1141 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
     1201 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
     1261 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
     1321 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
     1381 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
     1441 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
     1501 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
     1561 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
     1621 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
     1681 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
     1741 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
     1801 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
     1861 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
     1921 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
     1981 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
     2041 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
     2101 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
     2161 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
     2221 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
     2281 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
     2341 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
     2401 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
     2461 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
     2521 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
     2581 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
     2641 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
     2701 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
     2761 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
     2821 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
     2881 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
     2941 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
     3001 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
     3061 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
     3121 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
     3181 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
     3241 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
     3301 accttcactt gct
//
LOCUS       DACDCV010000164         3252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDCV010000164 DACDCV010000000
VERSION     DACDCV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.168"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..383
                     /locus_tag="IV041_002772"
     CDS             75..383
                     /locus_tag="IV041_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8383500.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            410..709
                     /locus_tag="IV041_002773"
     CDS             410..709
                     /locus_tag="IV041_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8383501.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            883..1434
                     /locus_tag="IV041_002774"
     CDS             883..1434
                     /locus_tag="IV041_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8383502.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1580..1873
                     /locus_tag="IV041_002775"
     CDS             1580..1873
                     /locus_tag="IV041_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383503.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1866..2162
                     /locus_tag="IV041_002776"
     CDS             1866..2162
                     /locus_tag="IV041_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383504.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2233..3237
                     /locus_tag="IV041_002777"
     CDS             2233..3237
                     /locus_tag="IV041_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8383505.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1220 a          454 c          649 g          929 t
ORIGIN      
        1 tatattatat ccatgaagcc caatcttctt taatagaaga ttgggctatt tttatggcaa
       61 aggagggaaa agaaatgttt caaaataaag gacaacgtta tgtcacaaag ggagtaatgg
      121 acagtttgcc ggtggaatta caagctttat gttggaatct aatcgatcaa aacgttcaaa
      181 agcaatttca tttagactat cttcaaatct ttgaattttc tacggaaaaa ggaaatcaaa
      241 agttggttca tcgtcaagaa gaaccagaag caagaaaaga gtatctgatt tcacctaagc
      301 ttcgtttgaa atcagtgtct caaaaaatat gggtcattga ttcgggtgag tatcaaacga
      361 tgttattacc agaagaatac tgatatctaa aagaaaggaa cgaaaggact tgttagaaac
      421 ggctatcgaa gtactggaaa agtgtgcaca gctagtcact actcctgaag agtggggcta
      481 tgaatccgtt acgatggaaa aagaggaaat tgaaatgggt atgcttcctg aagatgttac
      541 tctaccacgt ttagtgatga cacatcttta tatttattgc gcaccagaag atggaaaaga
      601 ctatgtagtt tatttcatta cagatattac cagccagcga gaatttgttc gtgggttact
      661 ggtagaagga cgattggttt ggagtcagat tggaggaacg aatgaataag atcgattatt
      721 tgagaatgat tgcgaaatgt ttagaagata tccttacagc gattcatcaa attctgaaga
      781 aaaaaggaga aagaatatga aatattttca actagtattg acacttataa cggcaatggt
      841 gattgaattg cttgaacaaa aattagcata ggagaaaaag agatgattga aaaagaatta
      901 gtgaccacac aaacagaagt ttggcgagat cctcatcata cccatcgcta tgttttcaaa
      961 cgacaatggg cgatcaaagg aaaagaagcg gaagataaat tagcggtagt cattactatt
     1021 cgtccaacag atacagaacc atttacgaat gatttgagta tgttattaat cgaaaagaat
     1081 atccgacaat taggatttac tggttttatt gctgtgaatc ttttttcgta tacaaaagcg
     1141 aaaagtcctc gggtatttcc tcgtggaaat gatgaacaat ctttggaaat attaacaact
     1201 gttttcacag aaaagaaaat caacaccatt atttttgctt gtggttcgat tacagcgacg
     1261 agtcaagtgg cttatgaaca agcgaagacc atttacgatc aattgagtgc gaaacaaaaa
     1321 aagatgacca aagtattgat aaatgcagaa ggtaagttat cccacccact aagtattcat
     1381 gttcgtaatg aatggcaatt ggcagatagt caattattat ttcaagaaaa ataacatttt
     1441 attttcttat gttacttata agtagtttta catgcttttt gactttaaaa atacatgtga
     1501 tgatacatgt tatatgaatt atcaatgtac ccagtaatat gctattttca ttatgtaaaa
     1561 aataacaggg ggtgtgtaga tggagattga tgaaacttgt ttgagtaact cttataaaag
     1621 tatgaatgac ttttttgagg aaatggaact ggattcagaa ttgctctatc aattttatct
     1681 tgcttttcga gggcaacaga tttcttttcc aatgaaaatg tacgatcgtg agttagttag
     1741 aaaaagaatc gaaaacatga tagaacagga aaaaactgta gatatccgtc aattgacaga
     1801 agtttatggt ttctctgcaa gatggattca aaaagtaatt aaacaaaaag aaaggagacg
     1861 agtacatggc taagctgatt gtattaggtg gtttaattta cggtggatat tggttatata
     1921 aatatatgct tgcgcatccg aatatagata aaggaaaagt aattaagaca gttggggtaa
     1981 tcatagttgg actcatagct ctaactaaga aaaataatac taatagccca aaaataatga
     2041 gaacttatcg ctgtagagga tgtggaaggg aaatttctac tagttttaat ccatctgaaa
     2101 gccctcaagg aagagatcgt tgtcatgctg gctatcgaca ttcttgggaa aggattaact
     2161 aaatgaacac cattgagatt aattgactga taaaataagt acgcgaggga gaaattgaaa
     2221 gggggaaata aaatgaaaaa atggttgtta ttactaattg gaatggggat tttattcact
     2281 ggttgtacat ctgagaatac gaggactaaa gagactgtag aaactgatac aacaattgag
     2341 gctaaaaaaa gtaaaacgca atcaactgaa caaactacaa cagaaatgaa acaatctgtg
     2401 gaagcaagta caagtgaaag ttcaatcatt gaatcttctt ctgaagaggt taaaaatact
     2461 ttgtgggata gtgagaaatc tactcaacta gcaatttttg tcccacaatg gggaaagaca
     2521 ttaggtcaag aatataaaag ctacaatcct cagaacaatg taagtctgta tggtacgcct
     2581 ttaccaagtg cagttattga tggaaattgg aaaatggcaa tcaatgaagc accagttacc
     2641 gtacaatggt cagaagatgg aactggtcat gcagatttca atttagtagc agtttattct
     2701 gatgtagaaa ctggagaata tttagggcag catgtatatt tctttggctt tcaaaatgga
     2761 caacctaaag tttatttaac gcaacaaaat caaggcaatg ataaaaacta catgtacttc
     2821 aatgaaactc agaaccaaca gttaaaacaa ggtttcattg atattgtgaa tggacaaact
     2881 cctcaaactc cagtagtaga agaatctact caacaagcaa caactacaac gacacaatct
     2941 acaagtagct cagcaatttc aagttgggaa gctgctaaag aaactgtact aaataataaa
     3001 gctttatggt tgtatgattc tcctgataaa aatgcatcaa atactaattt aattagcaca
     3061 aatgaatata tgaaagatct aatgcaagat gaaaatggaa gctactattc aattaatatt
     3121 gtaacggatg ataatggaaa tacaacaatg gctggtggaa aacaattccg tgtttataca
     3181 gatggaagaa tttctcaacg tattggaatg actaacaatt ttcaaatgat taaataattg
     3241 aaaatattat tt
//
LOCUS       DACDCV010000165         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDCV010000165 DACDCV010000000
VERSION     DACDCV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.169"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IV041_002778"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IV041_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IV041_002779"
     CDS             1360..1992
                     /locus_tag="IV041_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8383506.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IV041_002780"
     CDS             2197..2424
                     /locus_tag="IV041_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8383507.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IV041_002781"
     CDS             2493..2759
                     /locus_tag="IV041_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8383508.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IV041_002782"
     CDS             2749..3018
                     /locus_tag="IV041_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8383509.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDCV010000166         3123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDCV010000166 DACDCV010000000
VERSION     DACDCV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.170"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..750
                     /locus_tag="IV041_002783"
     CDS             61..750
                     /locus_tag="IV041_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8383510.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            764..2266
                     /locus_tag="IV041_002784"
     CDS             764..2266
                     /locus_tag="IV041_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8383511.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2279..2755
                     /locus_tag="IV041_002785"
     CDS             2279..2755
                     /locus_tag="IV041_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8383512.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2852..2962)
                     /locus_tag="IV041_002786"
                     /pseudo
     CDS             complement(2852..2962)
                     /locus_tag="IV041_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1027 a          416 c          614 g         1066 t
ORIGIN      
        1 tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt gtttaggata
       61 atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca
      121 ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt tgctttggct
      181 gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat tattgaaatt
      241 agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc accagaatct
      301 ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg tgttgaggta
      361 atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa ttcttttatt
      421 actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc tacatttgaa
      481 gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt aagtggatat
      541 actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa attggtagaa
      601 gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc aaaagagata
      661 aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc acaggaaatt
      721 gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta aaaaattttc
      781 acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg gattactatt
      841 gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat ttttagatca
      901 accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag tttttgcaaa
      961 cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag ataaaggaac
     1021 agctggttta gctggtggag tagcttatct agtatatact gcaactatta gtgggtttct
     1081 tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat caattgttgt
     1141 tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac cacaattctt
     1201 aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg caatcattat
     1261 tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag ttgctggtga
     1321 acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat tgcgtttaac
     1381 aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat ctttaggtgg
     1441 taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct ttgcccaact
     1501 agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg caggaagatt
     1561 tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg caattcctaa
     1621 gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat catttttaac
     1681 tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc tttatgtgat
     1741 tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata ttcgtgtagg
     1801 gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc aagggaatga
     1861 caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta tatatttctt
     1921 tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg aagaagaatc
     1981 agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag agtctcttat
     2041 cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg
     2101 tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa aagacactgg
     2161 tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag ggaaagctga
     2221 cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg tttttaatat
     2281 gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt ccggaaaaat
     2341 catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag gtgatggttt
     2401 tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag taacaaatat
     2461 atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt atttgattca
     2521 tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg ttcaagaaaa
     2581 tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa taataaaatc
     2641 tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca atgatttaat
     2701 aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag
     2761 tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa gttggtgaac
     2821 agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat tccccatgcc
     2881 aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca tttcgttgga
     2941 ataccttgat atgattattc aaatataaaa tatagagaga agagagagag ggaagatatt
     3001 tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca atttatattc
     3061 tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa tgctgaaaga
     3121 tgt
//
LOCUS       DACDCV010000167         3067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDCV010000167 DACDCV010000000
VERSION     DACDCV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.172"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV041_002787"
     CDS             <1..1076
                     /locus_tag="IV041_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383513.1"
                     /translation="QNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCVPQ
                     TINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADTDA
                     FIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDLLE
                     KLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVLLT
                     LVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLHDA
                     LQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDYPR
                     KILGYATPHDTFVQAFRQERLTA"
     gene            1297..2649
                     /gene="brnQ"
                     /locus_tag="IV041_002788"
     CDS             1297..2649
                     /gene="brnQ"
                     /locus_tag="IV041_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8383514.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          839 a          653 c          710 g          865 t
ORIGIN      
        1 cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac tcagaacgtt
       61 gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg gatgtcctgg
      121 gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact cagcttaacc
      181 gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac gccgatgccg
      241 ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta tgggccgaca
      301 cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg tccccggacg
      361 tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc cctgggacca
      421 ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg gacctgctag
      481 aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat aaacgggtgc
      541 tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc ttcggccatt
      601 gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg ctgacgttgg
      661 tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat gccgaatcgg
      721 tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc cagctgttca
      781 agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc ttgcaggatg
      841 tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg agcgaaaacc
      901 aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac gtgagcgaaa
      961 accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt ttgggctatg
     1021 caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg gcttgacacg
     1081 tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct ggccggggcc
     1141 agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg attagaaaaa
     1201 ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggctaactt aaacttgaaa
     1261 tttacgactt ttaaaaaaac cgtaaaggag ttatgtatga caaaaaaatt atcatttaaa
     1321 gactatcttt ttataggctc catgcttttc ggtctatttt ttggtgctgg aaatcttatc
     1381 tttcccgttc atttgggtca agaagcaggc gctgctacct tttgggcgaa tctaggtttc
     1441 ttggttaccg gtatcggtct tcccttctta ggggtgattg ccattggtgt atcgaaaagc
     1501 tcaggtgtat atgaattagc aaaacggata aatcaaacat atgcgatggt ttttaccatc
     1561 ttgctttact tagttatcgg accgtttttt gctttacctc gccttgcaac cacatctttc
     1621 gagattggtt tagcaccatt tatcgatcct agcagacaga cgctatatct agcaattttc
     1681 agtattgtat tttttgtgct ggcttggtgg ttttcaagaa aaccgacaaa gatattagat
     1741 tatgttggaa agttcctgaa tcctgcattt ttggttttat taggtgcttt gcttctcctt
     1801 gcttttttga atccactagg ttctgtaaat caagcgccta ttcagccaaa ttatcaagag
     1861 catgcttttt tcacagggtt tactcaagga tataacacat tagatgcgct tgctgcttta
     1921 gcatttggga tcatcattgt cacaacaatc caaaacatgg gtgtaacaaa acctgctgaa
     1981 atcgctaaag acacgatcaa atctggtgcg atcagcatta tattgatggg aatcatttat
     2041 actttgctcg cttatttagg tgctatgagc ttaggtagct ttgctctaag tgaaaatggc
     2101 gggatcacgc tggctcagat tgctgatcat tatttaggta cttatggcag tatcttatta
     2161 gcatttatcg ttatattagc ttgcttgaag accggtattg gtttgattac agcttttgca
     2221 gaaacattca cagatttgtt tccgcaaaga aattacgcat tctttgttgc acttgctagt
     2281 atccttcctt gtttagcggc aaacgtaggc ttgacgaata tcattcaatt ttcgttgcct
     2341 gttttgatgt tcatctatcc attagcaatg acactgattt tactagtgtt agttggtcct
     2401 ttgtttaagc agcgtacggc tgtttatcga atgactactt attttacatt gattgcttcg
     2461 atttttgatg ggttgaacgc ttgcccagaa actatcaaac aaacacctat tgttcaaaat
     2521 cttttatatg ttgcggaaag ctacttgccg tttttcaaat tggggatggg gtggatcgta
     2581 ccagcagtta tcggttttgt tatcggactg atttggagtt tcgctaagaa agaagaagta
     2641 gcagattgat aaaagaggtt gtgaaagaga cgttcagcct tgagcaataa aaagtaattt
     2701 tgaaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actgactttt
     2761 gaacaccgtt tattgagagg tcgtgaaaaa gccgcttagt ttcaagtatt aagaaatatt
     2821 ttgtgaaaat ggcttctcac atttttgcat aaatttgact taataccgag aaactggctt
     2881 ttgaacaccg tttattgagc gtctataaac tgacctgagt attagagaaa caaaacggca
     2941 aagtagctcc atatactttg ccgttttgtt ttgaactaat accgaagaga agctgatcaa
     3001 acaacatttc ttttataata tagattttag acatatatat gatataaaaa tagttttcgc
     3061 aaaaaaa
//
LOCUS       DACDCV010000168         3049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDCV010000168 DACDCV010000000
VERSION     DACDCV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.174"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            204..818
                     /locus_tag="IV041_002789"
     CDS             204..818
                     /locus_tag="IV041_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383515.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(983..1807)
                     /locus_tag="IV041_002790"
     CDS             complement(983..1807)
                     /locus_tag="IV041_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383516.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2445..2702)
                     /locus_tag="IV041_002791"
     CDS             complement(2445..2702)
                     /locus_tag="IV041_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8383517.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2695..2964)
                     /locus_tag="IV041_002792"
     CDS             complement(2695..2964)
                     /locus_tag="IV041_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8383518.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1018 a          520 c          399 g         1112 t
ORIGIN      
        1 ttttcttaat aaatcttaac agataaatca agttatatac tagaaaattc ataaaatata
       61 tttttaatgt cataatcgtt taaattttct tgattgacat ttatttacaa aaaatattgc
      121 catttaaaaa gaaaagtgac atatggcttc ctcgtgtatg attagggtgt attttaaata
      181 gttgcagaaa gtaggtaaaa gttttgagag ttgcatacat acgtgtctca tcgatcgatc
      241 aaaatgaaca acgccagatt gaagaaatga aaaaattcgg tgcagagaga attttcatcg
      301 agaaacaatc tggcgcaacg attactcatc gacctgtttt ccaagaagca ttagattacg
      361 tccgagatca agatattttt attgtggaag ctattgatcg acttggaaga aattatgacg
      421 agatcatcgc ttcagtcaat tacttgaaga agaaaaatgt gaagctgatc attactagtt
      481 taccgatcat ggctgaagca attggcaatc ctctcctgga taaattcatt aaggatctga
      541 tcatccaaat tttggccatg atcgctgaac aagaacggac tgaatcgaaa cgtcgccagg
      601 cacaaggaat taagatcgca aaagcaaacg gcgtctacaa agggcggccc aaattataca
      661 gtgcagacgc aaaagatccc caacgtcgcc ttgtttacaa aagcatcgtt gaagatctta
      721 aaaatggtgt tgcgattgct aagattgcta aagactacaa tgttacgaga caaaccgtgt
      781 atagaataaa aaaagacagc atggtcaatg acaaatagtt gatcatgctg cttttattat
      841 cttctcatct ttaatttcct acatataatt actatctata aaattaagtc ttactcagaa
      901 cagataaagc ataaaattcc aacaaaccat tttttactta actaaataaa gagtctgtta
      961 atataatctt cacttattta cgttaatccc ctactgtatt tgttggttcc ttatctatat
     1021 tttctacaaa atccttatta tcttgtgatt tagcttcgta taattctttg tatgtcgaca
     1081 cattggttat actaattcct atagctagtt cttgagactc tacttttctc tctagctcct
     1141 ttgacaaagt gatttgagat ttatttggtt ccgttggagt caattctata gattcttcat
     1201 aaaaatctct atacataaag ctatcattaa ttttataaaa aaaattaaat gtttttagca
     1261 ctgccatata acattcaaaa cattcttcta tagttggttt acttccttga ggagaatgaa
     1321 ccaatttatt acgtttttct atgaaatttt taatttcctt tcttatttct ttcggaaact
     1381 caatattaaa gtacccttta aacatgctcc ctaataattt gtctacaggg ggagattgta
     1441 aattctcaaa tattagagtt aaatcaggtt tctcttgttg ataaaattcc tttaccccta
     1501 tttcacaggc tgtaattatc gaaataaacc tatctctagt atttgatata tttttacttc
     1561 ttttaaggag tataagagaa aaggggatac tttcgtgtcc atgcatgtaa ttaactaatt
     1621 gttgagcttc agaagttata tctctaatta tatagttaac ggaattagga agtgtcttta
     1681 actctaatga aatttctatc caattactat cttctaatct gtatatgctc gaagtataat
     1741 ttgattcgtc agcaagcatc gctacatccc aaacactgat cataaaatct tttaaacgtt
     1801 caatcatttc agcaatattg ctaaaatcag ctaatttttc tttagaataa aaaaaacatt
     1861 tatatcagaa tcaataaatt ctattttttt tattactata tcgtcatcat tatcttgacc
     1921 atttatgttt attgttccct gagtggggag tacttctttc aaattcaagt taaatttata
     1981 ttccattact tctcctccaa tatataaaaa ttacttaact gtatccaaat ttaatatgta
     2041 aataaatcat taatttttag ctaaattcta tgagaagttt atttttgttg tttttgctaa
     2101 ttaactaaaa ctagtacaaa aaatttaaaa tagattatta aacctattgc ttattctatt
     2161 cagtcatata tataagcgtc atcctttagt aactaacatc ccacactaca atagtattat
     2221 aacatatcta ttcaacttca aattgttgaa atttaagcca tttatattta cttttcctca
     2281 actttagaaa tgattttact aaccttatat ccgtaatacg cgtaacaaac acattcatta
     2341 ataacaatca acactaagct atattagctt tttaaatgac tagggtaaat aaaataagga
     2401 tcaaaaactt ttaagtttct gaccctttcc ttacttccga ttggttaata gtgatctttt
     2461 gcagaataaa taaatatcgt ttcattttca actctatata tcagtctatg ttcatctgta
     2521 attcttctgg accattttcc agataaatca tgctttaatg gctcaggttt tcctaatcca
     2581 gcaaaggggg aacgatcgat atcttttatt aacttgttaa tcttttttat attgcttttg
     2641 tttccttgct catgccaata aagataatca tcccaagcat catcagacca agccttaatc
     2701 atcagattca acctcgatta agtcatgtgt tttaaatgca cctttggaga attgttcatc
     2761 tcctcgacga attttttcca tgacgtaatt attagaaagt gttctcaacg tttcttgcat
     2821 agaatcataa tctctttttg ataatacaac aactgtatct tctacatctt tacttgttac
     2881 aataagtgtt tcagcatcct cattaacttg tttcatataa ctacgtaaat tttggcggaa
     2941 atttgaataa gctactgctt ccattccttt caccccttat ttatttctat aaaacaattg
     3001 tacattatat tgtacaatta agcaaatgat taatattcta acttcttat
//
LOCUS       DACDCV010000169         3021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDCV010000169 DACDCV010000000
VERSION     DACDCV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.175"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IV041_002793"
     CDS             complement(287..649)
                     /locus_tag="IV041_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383519.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IV041_002794"
     CDS             complement(723..1103)
                     /locus_tag="IV041_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8383520.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IV041_002795"
     CDS             1185..2360
                     /locus_tag="IV041_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8383521.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IV041_002796"
     CDS             complement(2437..3021)
                     /locus_tag="IV041_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8383522.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          941 a          578 c          498 g         1004 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca t
//
LOCUS       DACDCV010000170         2988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDCV010000170 DACDCV010000000
VERSION     DACDCV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.176"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            612..>2988
                     /gene="esp"
                     /locus_tag="IV041_002797"
     CDS             612..>2988
                     /gene="esp"
                     /locus_tag="IV041_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8383523.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKD"
BASE COUNT         1090 a          453 c          588 g          857 t
ORIGIN      
        1 catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat
       61 aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg
      121 ttgataactc caatcattcg tttaagattc taggaatcat tcagaaatgc tgtgaaaccg
      181 atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct
      241 ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgttattt
      301 gaatactctg aagtttaatc taagaaaggg taatattttg aaaacatgct tcatcgcata
      361 gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg
      421 aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta
      481 atgttcatga aattctctaa gtaaattagt taacataagg ttagtaagat tcctattctg
      541 tgaataatac gtatcagatt tatcttttat tcttactagg aaagtaatta ttaagggagt
      601 tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac
      661 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg
      721 ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca
      781 gcccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
      841 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
      901 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
      961 aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat
     1021 tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac
     1081 ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt
     1141 atattcgtgc aagtgatgga ttatttgctg gggcagaaac aaccgatacg caaaccgctc
     1201 ctaatggggt aattacgttt gaaaagagcc tagatgaaat ttttaaagag ataggcatag
     1261 acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg
     1321 ataaaattgt accgggtatt gatagtacgg gctacttttt aacagattca gatgatttct
     1381 ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg
     1441 caaaatatga gaaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg
     1501 gagctataat cttagatcat aagttaacga aaaattataa tttctcatat tcatcttccg
     1561 caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag
     1621 cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcgtttgaat
     1681 ccggaacaaa agtagctgat ttagcaatcg agcgacggga aggtcatgaa aattatggca
     1741 taggttcaat aactaataat gatttaacta aacttatcga ttttgctaat gcaagtccac
     1801 gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag
     1861 atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg
     1921 attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt
     1981 attatttgca agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca
     2041 cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta
     2101 aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata
     2161 aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa
     2221 tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag
     2281 atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg
     2341 ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag
     2401 atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga
     2461 aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga
     2521 tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg
     2581 aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa
     2641 aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca atttcagatg
     2701 tctataataa gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt gttacattca
     2761 ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg
     2821 tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt
     2881 atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtaaccttc accgcagggg
     2941 aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacg
//
LOCUS       DACDCV010000171         2957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDCV010000171 DACDCV010000000
VERSION     DACDCV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.177"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..369
                     /locus_tag="IV041_002798"
     CDS             13..369
                     /locus_tag="IV041_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383524.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            372..1529
                     /locus_tag="IV041_002799"
     CDS             372..1529
                     /locus_tag="IV041_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383525.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1598..2938)
                     /locus_tag="IV041_002800"
     CDS             complement(1598..2938)
                     /locus_tag="IV041_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8383526.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          945 a          502 c          595 g          915 t
ORIGIN      
        1 ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt atatcaattt
       61 ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat cgctttagat
      121 ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc gttttgcgaa
      181 aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa agtcgagttt
      241 tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac gattggttat
      301 attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct tagtcaaaaa
      361 attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact attttgaaaa
      421 actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa gaataccgta
      481 tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat tctgatatat
      541 tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat gcgccatata
      601 cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg caacgaatga
      661 aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg catccattga
      721 taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca aacttctggt
      781 cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt ggtgtaatga
      841 acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa agttggggct
      901 ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt tttacaactt
      961 cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca agggtttttg
     1021 ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat tttcagcata
     1081 tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct cgcagaattc
     1141 cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac tgtatgttag
     1201 tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa tcaaagtatg
     1261 gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa cgcccaataa
     1321 aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat cctatcgaca
     1381 aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa cctacaaaaa
     1441 atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa aatagatcca
     1501 tattggaaaa attggatttc gttaattaag aaaagcggct cttcgtcaat gagtgttggt
     1561 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
     1621 gtgttgttgt tctggcttta gtcagttggg tagtgttctt agttttcttg aaatattttc
     1681 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctaaagcca taggcaattc
     1741 gttttagtac tttgatatgg ttgttcaaac attctatagg gccgttggaa tagggaactc
     1801 gcaacgctcg tttgatttct gttcgatatt tcttgaaggt cgtgaacact ggttgatagt
     1861 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
     1921 ttctgatagc ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag gagtcatcat
     1981 aatataatag ccgatcgact atctccgctt ctgtcagatg atctcgaaat gacggacgcc
     2041 agtagcgatg ctgaccattc aattcccacg cattcttttg aagtagtctc cagtatttct
     2101 ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt
     2161 tggtaaaggc ccgaccgatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat
     2221 tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac
     2281 gtacttgttc acggactttt ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt
     2341 gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca
     2401 tggctcccga gacgtttttc accgatttga attcatcgaa acacaccact tctggtaacg
     2461 gctggttcaa agaaacggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg
     2521 gagaaatgtg cttcatacgt gcaatcaaag acatagaaat aggctcactt aatagctcaa
     2581 gaatggcttg tttgactctg cgggcaatgg agcagcgacg atcgctaact gaatcttctg
     2641 ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg
     2701 tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc catttaatga
     2761 tcgtttgctt attttgatag tggcaatgga agcattcctc tggttgatag gtcaatgtgc
     2821 catcaaaaac aaaacatgtt tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa
     2881 aggtaagatt taggtctaga atatctagta actctttggt aaactctgaa taggacatct
     2941 tgaaacactc ctttgaa
//
LOCUS       DACDCV010000172         2880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDCV010000172 DACDCV010000000
VERSION     DACDCV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.179"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            802..1068
                     /locus_tag="IV041_002801"
     CDS             802..1068
                     /locus_tag="IV041_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383527.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="IV041_002802"
     CDS             1126..1638
                     /locus_tag="IV041_002802"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383528.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="IV041_002803"
     CDS             1635..2672
                     /locus_tag="IV041_002803"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8383529.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          508 c          573 g          758 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac cgaccatcgt ccagtaaaga gacgcaataa attctatcga agtttacgca
//
LOCUS       DACDCV010000173         2817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDCV010000173 DACDCV010000000
VERSION     DACDCV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.180"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(846..2186)
                     /gene="glnA"
                     /locus_tag="IV041_002804"
     CDS             complement(846..2186)
                     /gene="glnA"
                     /locus_tag="IV041_002804"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8383530.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2214..2606)
                     /locus_tag="IV041_002805"
     CDS             complement(2214..2606)
                     /locus_tag="IV041_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8383531.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          844 a          607 c          455 g          911 t
ORIGIN      
        1 tagataaaaa aataaaacaa acattatgat agataatatc ccacctaaca aagaactact
       61 ttttcctact tgcagcaaca gtttatatag cttatcgtag aaacaatctt tttctttcac
      121 ccttatttac ctcctttacg taaaaaacgt agtccacagt tctcggattt ttggcatatc
      181 ttttctatta aataatatca aaaacaaaac aattccacta attttttcaa agatgacctt
      241 tttttcaaca aaagtgcctt gtagggattc attgaaggta acgaatcaca taagtaaaaa
      301 ataaggagat gcgacaaaag tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc
      361 aactcctcgg tcacaagtca caatatccat caaacatgag aagctgtttt tgaaattatt
      421 tcttgtgatt cggagttaaa tggtaggctc acaacctctg gataaacggt attcaaaagc
      481 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
      541 tttcttaata ctcgaaacta aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa
      601 gcttgcatct gcggtaataa gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg
      661 gctattctta ttacttgttg cagggcagct ttttcacaac ctctccacta aacggtgttc
      721 aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttccg
      781 ttagtttctc taatactcag gtcaaaacgc tgatttcaca gcctctaatt cacggtattc
      841 ctgttttaat aaagttctag atattgttct ctttcccact cggaaacagt ttgtctgaat
      901 gccgcccatt cgagacgttt tgcttcaaca aaatttgaga agatatgttg tcctaatgcg
      961 tcaatcatta cattatcctt acgcaattct ttgatagcat tatggatagt tgaaggtaag
     1021 tcctggatat gtgcagcttc tctttcttcc tcatccatta cataaatgtt acggtcaaca
     1081 gctgctggcg gtgtgatctg attacggata ccgtctagtc ctgcttgcaa caatactgcc
     1141 attgccaagt atgggtttgc agatggatca actgaccgca attccaaacg tgtggataaa
     1201 ccgcgagatt ctggtacacg tactaatggc gaacgattac ggccactcca tgcgacataa
     1261 acaggcgctt cataacctgg aacgagacgt ttataagaat tgactgttgg attgcaaacg
     1321 gctgtgtagg cacgagcatg tttcagcaaa ccacctaaga aatgataagc tgtttcactt
     1381 agttccaatt caccgtcttt atcatagaaa acatttcctt catcattgaa caaagacata
     1441 ttgcaatgca taccagaacc attgatccca aataacggtt tcggcatgaa agtcgcgtga
     1501 agtccatgct tgcgtgcaat cgttttgaca acgagcttga aggtttggat attatcacaa
     1561 gcatctacca catctgcata tttaaagtca atttcatgct gtcctggcgc tacttcatga
     1621 tgtgaagctt ccacttcaaa gcctagactt tctaattcca acacgatgtc acggcggcaa
     1681 ttttcaccta aatctgttgg tgcaaaatca aaataaccac ctttatcatt caaggtcgtt
     1741 gtgatcccac cgtcttcgtc taatttaaat aagaagaatt ctggttcagg tcctaaattg
     1801 aaagaggtaa aaccaaggtc gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga
     1861 tcgccggcaa atggtgtccc atccggatta tagatatcac aaattaaacg agcaactttt
     1921 ccatgagcac tttcccacgg gaagatcatc caagtggaga gatctggata caaatacata
     1981 tcgctttctt caattcgaac aaatccttca atcgaagaac cgtcaaacat cattttattt
     2041 tctaatactt tgtcgatctg gctcactgga acttctacat ttttgatcgt acctaagatg
     2101 tctgtgaaca tcaatcgtaa aaatcgtacg ttttcttcct caatgattcg tttgatttct
     2161 tcacctgtta tctgtttctt taccatggta tccctcttcc ttttttaatt tattcagtca
     2221 aagaattgtc tttgcaatcc ttgcgactga taatgacctg ttttgcttaa cccaccttgc
     2281 gccaaaatct catcacgaaa aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt
     2341 tttgcttctt gttttttcgc ctgtgccatt tcgtatacac gtttgatccc agccatattc
     2401 aatccctctg aaagatagtc tttaatctca agcaacatat caatatcatt cagcgaatac
     2461 atacgtcggt ttccttcgct tcgttccgga tgaactaaat cttgttcttc ataatagcga
     2521 atttgccgag cagacaaatc agtcagcttc ataactgtac caattggaaa tacggacata
     2581 gatcggcgca attccttttc tcccatcaga cgtccccttt cgttttataa gttggctaaa
     2641 gtgtaccaat taaaaaaaat attgtcaact gcatatgtta ggttttctaa catgagatct
     2701 ctatttttta taaaataaca aaaaaataag aacacaaaaa aggtatctag ttgattttcg
     2761 taccagatac cttttatttt ttattatact gtactatcct ttattagtgt ttttatt
//
LOCUS       DACDCV010000174         2735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDCV010000174 DACDCV010000000
VERSION     DACDCV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.181"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            229..573
                     /locus_tag="IV041_002806"
     CDS             229..573
                     /locus_tag="IV041_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8383532.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            574..993
                     /locus_tag="IV041_002807"
     CDS             574..993
                     /locus_tag="IV041_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8383533.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1085..1435)
                     /locus_tag="IV041_002808"
     CDS             complement(1085..1435)
                     /locus_tag="IV041_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383534.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1401..2621)
                     /locus_tag="IV041_002809"
     CDS             complement(1401..2621)
                     /locus_tag="IV041_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8383535.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1013 a          491 c          533 g          698 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtaaaata
       61 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
      121 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
      181 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
      241 caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga attgaatgat
      301 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
      361 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
      421 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
      481 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
      541 gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga agaaatacac
      601 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
      661 caaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
      721 atttttgaac caggttgtcg caatcactgg catgtccata aagacggcta tcaaatctta
      781 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
      841 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
      901 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
      961 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
     1021 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
     1081 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
     1141 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
     1201 gtcggggatt acctaatagt tcttgatagt agatttccat actagggtcc cgaggattgt
     1261 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
     1321 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
     1381 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
     1441 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
     1501 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
     1561 tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc gtaaaaaccg
     1621 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
     1681 tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg aaaactgttt
     1741 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
     1801 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagcct
     1861 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
     1921 gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata gaaaccaatt
     1981 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
     2041 aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
     2101 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
     2161 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
     2221 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
     2281 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
     2341 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
     2401 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
     2461 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
     2521 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
     2581 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
     2641 cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt ttttgaggat
     2701 gaatataaat aacgtcagtt tcgttttcca ttaca
//
LOCUS       DACDCV010000175         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDCV010000175 DACDCV010000000
VERSION     DACDCV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.182"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..121)
                     /locus_tag="IV041_002810"
     CDS             complement(<1..121)
                     /locus_tag="IV041_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HAP8383536.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYN"
     gene            191..541
                     /locus_tag="IV041_002811"
     CDS             191..541
                     /locus_tag="IV041_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8383537.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            955..1773
                     /locus_tag="IV041_002812"
     CDS             955..1773
                     /locus_tag="IV041_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8383538.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1846..2205)
                     /locus_tag="IV041_002813"
     CDS             complement(1846..2205)
                     /locus_tag="IV041_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8383539.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          908 a          396 c          492 g          936 t
ORIGIN      
        1 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatggca accggagttt
       61 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      121 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      181 gcttttttat ttgtctcgct taattataca aacggcgttt tataaagcaa tcattcctga
      241 gaacttagat gaggttaaac tttatttatg ggaagataac ggagaaattg tcggttttag
      301 tggtgtaagt gcagaagaat tagttatgct atttcttgat ccagatttta ttggtaagcg
      361 atatggtagt caaatattaa ttgagctgat tgaaactgaa gatataaaaa gaattgatgt
      421 taatagtcaa aataaatatg caaaacattt ttatttaagc catggatttg aaattgaatc
      481 tgaagaagac ttagatggtt ttggtaaacc ataccctata acgcatttag taaaaaaata
      541 attcatgaat ggtttattac ctgattgaac atgaataaaa gtaatatttt tattttgatg
      601 gcctatttct aggttatgaa ttatccagat caaatttgtg tattacattc agtaataagg
      661 tgcttatctt aggatcaact tatgatacta gctgaccaag gatgtaccag ttatagaaaa
      721 tcagttaaag taaaaatgat aaaaatctta tgtcaacact gatttaggct gacctgtggg
      781 ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat ggcttgtatg tatctagata
      841 gttaactgaa attagaaatg ttcttatttt taacggagta agaaaaatag gctgctttag
      901 gtctgaatac tttttcaata taatgaagat atcaaaggcg acaggagtgt ttttatgaat
      961 aagaatcctc ataatctaaa gttggtagca gtagatatgg atggaacatt tgctcgtact
     1021 gattacacat acgatatccc aaggttcaag gctgttttat cgcgaatgaa agctgttgat
     1081 tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat tgagatcgtt atttccaaat
     1141 tattatgatg aattaagttt tgttgctgaa aatggtgctt ttgttaaaga taaaacagag
     1201 gtactatttt cagccgatat gcctagaaaa gcgatcgagg tggtccttga tttatgtaaa
     1261 gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata gtgcctactg tgaaagagga
     1321 aaggtgagtc aagcattttt tgatctaacc aatatctatt accatagatt agaatgggta
     1381 gctgattttg cagaagttca agataaaatt ttaaaatttg ctccgactat gcctgttgaa
     1441 aaaaccgaat tttacgttga attgttccgt gaaaaattag ccgggctatt agtacctacc
     1501 agtagtggtc atggttctgt agacttgatt ctttctgagt gccataaaac tttcggcttg
     1561 aaaatgttag ttgagcgctt ggggattaat cctgaccagt gcgttgcttt tggagatggg
     1621 ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt atgaaatgga taatgcaacg
     1681 gaagctgtta agcaagttgc taagcatcaa tgtccttcaa atgacgaaga cggtgtttta
     1741 gtgacgctcg atcgattgtt ccctaacacg taaacactag gtaattcatc aaaaaacctc
     1801 gatataagaa atattcgagg ttttaaaact atatttagaa cagatttacg aagcgtctga
     1861 tgagtcaaaa tagacttgga cattgctatc taaaatacca ctttcattct ttaaacgaat
     1921 tgtttgattt gatggagtta cctctaatac taaatgccca ttaataaagg tagtatcgta
     1981 ttcagatgtt ttaactgttg cttcttcgca aggtattgtt aaatcatatg ctggaatata
     2041 atgttgtgtg gaaactaata aaaaatgatc accaatcata cagcatccct ctgctgaaga
     2101 aaattgatta gagccatcat ttaaagttaa caagaatcca tcctttaaac gaggtacttg
     2161 cttaaatact tctggttgta cttctaaaaa taaattatcg ctcattttca cgaccaccat
     2221 cgacttccac agttgaaatt gacattttat ttaacacacc tattccccct taactataac
     2281 tcaattctat ataaaaaata ttctattctc aaataattga acttttcaaa tcgccaattt
     2341 acttcggctt tatcagagct cattcatatc aagagcgatt aacgctgtaa aaattaaacc
     2401 gaagatctga taaattttct ctattttcta caatatttaa aacgcttcct tagtctatta
     2461 tatatctatt tctcttagga gtatgtttag atgtttgaat aatagttgcc caactttaaa
     2521 ggttattctt ataatatttt acttcatttt ccaatagaaa attcaaagga ttactggttt
     2581 ttctaaaata gtttagttta aatacataag tatcctccga cactgtatta gttcataatc
     2641 atctaaatac aaaattttca aataaaaata tttcaagctc tatcgttgac gaatgtaaac
     2701 gatacttata aagtaataga aattgattac at
//
LOCUS       DACDCV010000176         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDCV010000176 DACDCV010000000
VERSION     DACDCV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.183"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IV041_002814"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IV041_002814"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8383540.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1067 a          421 c          558 g          679 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaact
//
LOCUS       DACDCV010000177         2715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDCV010000177 DACDCV010000000
VERSION     DACDCV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.184"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..378
                     /locus_tag="IV041_002815"
     CDS             1..378
                     /locus_tag="IV041_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383541.1"
                     /translation="MEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANT
                     PEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYI
                     VKAKVSKRFIFNPKLEIYSVVIE"
     gene            703..1320
                     /locus_tag="IV041_002816"
     CDS             703..1320
                     /locus_tag="IV041_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383542.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1361..1660
                     /locus_tag="IV041_002817"
     CDS             1361..1660
                     /locus_tag="IV041_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383543.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1694..1861
                     /locus_tag="IV041_002818"
     CDS             1694..1861
                     /locus_tag="IV041_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8383544.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1906..2511)
                     /locus_tag="IV041_002819"
     CDS             complement(1906..2511)
                     /locus_tag="IV041_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383545.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          951 a          409 c          495 g          860 t
ORIGIN      
        1 atagaatgga ataatctgac tcaaaataat aaaaataatt ctggatgtgg aattatcttt
       61 atgtttgtta tagttttggg aatagtagta cttttaataa ctgtcaagat agtagattac
      121 tttgcgaata ctcctgaaga acaaatacag agaaaacttc ctgagtttga aagagtacca
      181 ccttatgatc caagagataa ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa
      241 atgtggtttg aagttacagt tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat
      301 tatattgtaa aggcaaaagt atcaaaaaga tttattttta accctaaatt agaaatatat
      361 agtgttgtaa tagaataata gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg
      421 atgtgaaaaa taaaatatac catggaagac ataaaagaaa aactggataa ctagtttatt
      481 taatggcaga ccgttaaggc ctgccatttt tatgtatttt agcggaataa atataatcat
      541 gcgggagtgt aaacttactg tttcaaaaag tatacttttt gacatgcctt gattttcttt
      601 gtttatgtat ggcataataa ccacataata caatttgaaa cgtttctgat ttaatctgat
      661 tgttttgaaa caattttagt gaaaaaaggg tagataaaaa cgatgagaaa atttttttat
      721 atccgggtca gtagttatga tcaatcgatc gaacgacaac tgatttccgc gaaggaatta
      781 ggaatccctg aagaatatat atttgtagaa aaagccagcg gcaaagattt taaacgccct
      841 gaatatcaat taatgaaacg gatgtttcga aaaggagatt tactctatct acaatcatta
      901 gatagactag gcagaaataa acaaatgatt ttagaagaat ggcgagaatt aactaaggaa
      961 aagcagattg atatttctgt gttagacatg cctctgttag atactgctag ttacaagcaa
     1021 ctagatgggt tagagacgct tatctctgac ttaattctgc aattactcag ctatatggcc
     1081 gaagacgagc ggaaacgaat taatagccgg caaagagaag gcgtagaagc tgcgaaacaa
     1141 aagggtgtaa aattcggcag aaagaaaatt gctttacctc ctgaatttcc tgcgatctat
     1201 tctgactgga aagcaggaaa gataactgca gtctatgcta tggatcaatt gggcatgaaa
     1261 cgaaatacct tttatcgacg ggtgaaagaa tatgaacata ccttgcacca cttttcttga
     1321 tagatataac agctattaga cgggaaggga gagtttgaaa atggacttaa cgaatcaaag
     1381 ggtcgttttt actggaacgc tgcagcaaat gacacgaacc caagccatcg gtttggttca
     1441 ctctttaaac ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg
     1501 tcagtattct aagtcgttgt ttgatgattc tctctataca aaaaaggaac aaaccgcgcg
     1561 ggacttgatt gcgttaggtc atgagattac cattttatca gaagtcgaat tttatgcgtt
     1621 aattagtcaa cagttgaaag aatggcaacg gtatttataa tcgttagtaa aatttacaag
     1681 gaggaaaatt tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa taactagttt
     1741 taatcgaaat attgcttttc acgtggagtg tgactgtggg gaattagcaa aaagtgttta
     1801 tcaaaaaaat tacttttact gtcctaaatg ccatcgccga tacggggtac atggaacata
     1861 gaaacgaaat agtgcggtag aaaagcgaat gaatgtagta tatttctaca aatcattcgc
     1921 tttctttatt ttataaatag ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac
     1981 tggaatgcct tgctttaaca tgtccaccac ggttttaaaa actaagcgtt tttgcggatc
     2041 tgttgagtta atagaatact cgattggtcg tcccttatat cggccggctt tttgagccaa
     2101 taatactcct tgccgctgac gttcacgaat tttctcacgc tcgttttgtg caatataact
     2161 tagcagtgat aaaaacatat cgaacatagc ggtatccagg agttcatttc cagtattaaa
     2221 atctaaaaac tgtgcatcta atatttttaa cgctactttt ttctgtttca taaaagcaac
     2281 tgtatttttt atatcttcat aatctcttcc taaacgatct agtgatgtaa cgatgacgca
     2341 atcaccttca cggatataat ttagtaattt ttgaaactca ggacgctcag tatctttacc
     2401 actggctttt tcttggaata atttatcaat gttaagtcct tccatcattg ccaattgtcg
     2461 atccaaattt tgatcttgtg aagatactcg aatatagcca atttttccca taaaaaactc
     2521 ctttttaaaa gtgatataat ttacatgtaa aatttacttg tagttttact gctatcatag
     2581 catttttaaa aataaatttt atgtagactt aaaatttact tcatggaggg tttaaatgga
     2641 tgaaatcaaa agtaacaaaa aagcatttgg catttattta ggagaaccta gtacagaaca
     2701 attaaaccaa ttttt
//
LOCUS       DACDCV010000178         2645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDCV010000178 DACDCV010000000
VERSION     DACDCV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.185"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IV041_002820"
     CDS             complement(<1..461)
                     /locus_tag="IV041_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8383546.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(747..1034)
                     /locus_tag="IV041_002821"
     CDS             complement(747..1034)
                     /locus_tag="IV041_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8383547.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1024..1353)
                     /locus_tag="IV041_002822"
     CDS             complement(1024..1353)
                     /locus_tag="IV041_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8383548.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1430..1702)
                     /locus_tag="IV041_002823"
     CDS             complement(1430..1702)
                     /locus_tag="IV041_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8383549.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1702..1965)
                     /locus_tag="IV041_002824"
     CDS             complement(1702..1965)
                     /locus_tag="IV041_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8383550.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(2071..2487)
                     /locus_tag="IV041_002825"
     CDS             complement(2071..2487)
                     /locus_tag="IV041_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383551.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(2493..>2645)
                     /locus_tag="IV041_002826"
     CDS             complement(2493..>2645)
                     /locus_tag="IV041_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8383552.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT          806 a          514 c          339 g          986 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
      781 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
      841 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
      901 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
      961 tctttcaatg gcttctcttt tagctttttt aaatacttaa tgacatgttt tgaaggaacg
     1021 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     1081 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     1141 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     1201 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     1261 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     1321 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     1381 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     1441 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     1501 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     1561 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     1621 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     1681 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     1741 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     1801 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     1861 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     1921 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     1981 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     2041 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatttt tattaacaag
     2101 ttttatcaca ggcttcatat atttgattat aacttcatct tctacgtaga tatctgttat
     2161 cattacgaca tctgtactat ttcgattgag tactttcact tgatctccag cattgatttt
     2221 ccctctaaat cttttaggaa tgaaccaact atacatatga ttagcatcac caaaatgtgc
     2281 ttttatcgca ttctcaggat ataacttatt caatagttca ttctttttaa ttttcaaaac
     2341 ttcaaatcta tacttatcat catctgctaa tctaaataaa tcttctaaac gcatgttttc
     2401 taactgtaag ttttgaaaat cttccggtat aaattttcgg ttctgttgcg aagtttgaaa
     2461 atcaaacgcg ccctctctga actccaaata tcttaggctg ttattcccat taataccttg
     2521 atttcagtag acaccgaaaa gctgaagagc gttccatttc ttcggttctt tttatatatt
     2581 cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg tacggagact ttgataaaat
     2641 ttatt
//
LOCUS       DACDCV010000179         2627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDCV010000179 DACDCV010000000
VERSION     DACDCV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.186"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..371)
                     /locus_tag="IV041_002827"
     CDS             complement(81..371)
                     /locus_tag="IV041_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8383553.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(507..686)
                     /locus_tag="IV041_002828"
     CDS             complement(507..686)
                     /locus_tag="IV041_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(683..1033)
                     /locus_tag="IV041_002829"
     CDS             complement(683..1033)
                     /locus_tag="IV041_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383555.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1026..2351)
                     /locus_tag="IV041_002830"
     CDS             complement(1026..2351)
                     /locus_tag="IV041_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8383556.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          788 a          513 c          397 g          929 t
ORIGIN      
        1 tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata
       61 ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg
      121 aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa
      181 ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt
      241 cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct
      301 acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta
      361 ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta
      421 acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga
      481 taactctata gaatccgtaa aataccttat atatataacg ggaaaatata aaacctaaaa
      541 ttatgataac ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt
      601 atcaaacgtt ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt
      661 agctcatatt gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt
      721 cgatatgacg tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat
      781 cactacctaa gatcttgcca acaatatctt ctggatagta tccttcagcg tttcgttctt
      841 ctttttgaat agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct
      901 cttcagtcgt catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat
      961 caatttcttc agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat
     1021 tgtaatcaat catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt
     1081 ctagtaatgc ttctagcgct ttctaacatt gaagaagcat gtacaatcga cgtaaaacca
     1141 tatttttgcc gaatcttgtc aataatttta tctaaatttt cttcgttaat ttgcttttgt
     1201 ggatcactaa ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt
     1261 ccaacgtgcc ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc
     1321 tgcaattcct ttgtattatc tgtgataggt attttcatct ggtgtgaaaa tcctggtctc
     1381 gtttctaaga tcgacgtacc aatgtttaaa tgaacacatc ctgttttaca attatgctgc
     1441 ctgatacgaa tggctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt
     1501 ctagtataat ctcgtggtaa cacttgtgaa ttaccataag atttttcctt tgtcggttct
     1561 ggcgggattg ctatatctgt tcgatcgatt ccgttagcat ggaagtacag ctgtaatcca
     1621 atcacaccta atctattctt aatcgtataa ggattccagt tagcaaggtc tctaatgctc
     1681 aaaattccca tttggttgag cctttttttc attcgagaac caattcccca aaagtccgtc
     1741 atttcgggaa tattccatac tttctcagga acgttttcgt acgtccattc cgcaataaat
     1801 ccttcattat gctttgcttc attgtctaag gctaatttag ccaaaagggg attatcgccg
     1861 acgcctaccg tagcaatcaa acctaactct tctttaatac gttcttggat catttgagcg
     1921 agcttcttcc ttcgctgact tcgtgtttct tcagtcgtaa aaagattcag tgattttgtc
     1981 acttttaaaa tcgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt
     2041 ctgaaaatat tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca
     2101 acgtgtagtg ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata
     2161 ccataccgct tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca
     2221 gatcgactca taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg
     2281 acacttgcgt aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa
     2341 taatccatca taaataacac tccattcttc cacattatac aaacatatgt tcgatgttgt
     2401 aaagaagaaa tagctaaata cataaaaaaa gaagcatagg tatgctatca ccctgcttct
     2461 tcttatgatt tgtatgatca atttattcta tcactttact aattaatttg aaaatctaat
     2521 aaaaactctc actctaaaag agtgagagac tgcaaattag agtacggact tttttggagt
     2581 tccgttcttt aatattaaca aatgaataaa atccttacaa catttga
//
LOCUS       DACDCV010000180         2544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDCV010000180 DACDCV010000000
VERSION     DACDCV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.187"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..283
                     /locus_tag="IV041_002831"
     CDS             32..283
                     /locus_tag="IV041_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8383557.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            385..2382
                     /gene="tkt"
                     /locus_tag="IV041_002832"
     CDS             385..2382
                     /gene="tkt"
                     /locus_tag="IV041_002832"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8383558.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          850 a          466 c          578 g          650 t
ORIGIN      
        1 aatatgaagg agagtaaaag gagggctgag catgctgtca cctgaaaaaa tagagcggat
       61 caatgaactt gcacgtaaaa agaaggaaaa tggtctctca aaagccgaag aacaagaaca
      121 aatcaacttg cgcaaagagt atttggaagc ttttcgttcc ggtatgcgtc atcatatcga
      181 aggaatgaaa gtcgtcgatc ctgagggcaa tgacgtgacc ccggaaaaat taaaagaaat
      241 ccaaaagaaa aaaggcttac acaaccgaaa tgacaacttt tagtaaaaaa tactgaaaaa
      301 gattgctttt tcgtgtgaca tcttatagaa tgaaagtgta agaaataaag gtaaaaaaaa
      361 cctaaattaa taggaggggt tattttgttt gacaaaattg accaacttgg cgttaacaca
      421 attcgtacat taagtattga agcagtccaa aaagcaaact caggacatcc tggattgccg
      481 atgggcgcag cacctatggc atatgcgtta tggacaaaac acctgaaagt aaatccaacg
      541 acatcaaaaa actgggctga ccgcgaccgt ttcgttcttt cggcaggaca tgggtcagca
      601 atgttgtata gcttgcttca tttagctggc tatcaagtga caatcgatga tttgaaacaa
      661 ttccgtcaat gggattctaa aacaccagga catcctgaag tacatcatac agatggtgta
      721 gaagccacta cagggccatt aggacaaggg attgcaatgg ctgtcggtat ggcaatggca
      781 gaagcgcacc ttgcagcaac gtataacaaa gaaaacttta atgtgatgga tcactatacg
      841 tatgcaatat gcggtgacgg agacttgatg gaaggggtat ctcaagaagc aagttcgatg
      901 gcaggtcata tgaaactagg gaaattgatc gttttatacg attcaaacga tatttcacta
      961 gacggtccaa cttcaaaagc tttcacagaa aacgttggtg ctcgctatga agcatatggc
     1021 tggcaacata tcttagtgaa agacggaaat gacctagaag caatctctaa agcgattgaa
     1081 gaagcaaaag ctgaaacaga caaaccaaca ttgatcgaag taaaaacagt catcggctat
     1141 ggtgctccta aagaaggaac atctgctgtt catggtgctc ctcttggtga agatggcatc
     1201 aagatggcta aagaagttta tggttgggag tatccagatt tcactgttcc agaagaagta
     1261 gctgcccgct tccatcaaac gatgatagaa gaaggacaaa aagctgaaga tgcatggaat
     1321 gaaatgtttg ccaattataa aaaagcttat cctgaattag cacaacaatt tgaagatgct
     1381 tttgatggca aactaccaga aaactgggat gcagaattgc caacttatga agttggaagc
     1441 agtcaagcaa gccgtgtttc aagtaaagaa gtcatccaag aattatcgaa agctatacca
     1501 agcttctggg gtggttcagc ggacttgtca ggttccaata atacaatggt ggctgcagat
     1561 aaagatttca ctcctgaaca ttatgaagga cgcaacatct ggttcggtgt ccgcgaattt
     1621 gcaatggctt cggctatgaa tggtatccaa cttcatggcg gaactcgtat ctacggtgga
     1681 acattctttg tctttgtcga ttatctaaga ccagcagttc gtttagcagc aatccaacat
     1741 actcctgttg tttacgtctt aacacatgac tcggttgctg tcggagaaga cggaccaacg
     1801 cacgaaccga ttgaacaact agcatctgtt cgctgcatgc ctggtgttca agtgattcgt
     1861 ccggcagacg gaaatgaaac acgtgctgct tggaaagtag cgatggagac aacagatgcg
     1921 cctactatcc ttgtattgag tcgtcaaaac cttccagtat tgccttctac aaaagaagta
     1981 gcagatgaaa tggtgaaaaa aggcgcgtat gttctttctc catcacaagg agaaacacca
     2041 gaaggtattt tgattgcaac aggttctgaa gttgatttgg ctgtgaaagc tcaaaaagaa
     2101 ttggctgaaa aaggcaaaga tgtttctgtt gtttctatgc caagctttga cctctttgaa
     2161 aaacaatctt ctgaatataa agaaagtgta cttcctaaat cagtgaagaa acgtgtggct
     2221 atcgaagctg ctgcatcatt tggatgggaa cgctacgtag gaacagaggg cgcaacgatt
     2281 acaatcgatc atttcggtgc atctgcccct ggcactaaaa ttttagaaga atttggattt
     2341 acagtagaaa atgtcgtgaa tacgtacaat caactatcat agatccaagc aattgtttga
     2401 ctgataagaa cagctgtctg caagttggag acagctgttc tttatgtgtt agaagaataa
     2461 atcaatatta cttttcgttt tcggctatgt tacatttaaa tgacaaaaca aagaattctt
     2521 cggattagtt tgtgtttctt gaga
//
LOCUS       DACDCV010000181         2424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDCV010000181 DACDCV010000000
VERSION     DACDCV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.188"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..2274
                     /locus_tag="IV041_002833"
     CDS             121..2274
                     /locus_tag="IV041_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8383559.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          425 c          536 g          635 t
ORIGIN      
        1 aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca ttttttcttt
       61 tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa
      121 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
      181 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      241 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      301 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      361 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      421 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      481 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      541 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      601 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      661 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      721 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      781 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      841 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      901 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      961 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
     1021 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
     1081 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1141 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1201 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1261 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1321 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1381 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1441 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1501 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1561 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1621 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1681 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1741 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1801 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1861 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1921 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1981 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     2041 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     2101 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2161 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2221 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2281 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2341 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2401 gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DACDCV010000182         2422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDCV010000182 DACDCV010000000
VERSION     DACDCV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.189"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..1183)
                     /locus_tag="IV041_002834"
     CDS             complement(44..1183)
                     /locus_tag="IV041_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8383560.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(1297..1881)
                     /locus_tag="IV041_002835"
     CDS             complement(1297..1881)
                     /locus_tag="IV041_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8383561.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(2008..>2422)
                     /locus_tag="IV041_002836"
     CDS             complement(2008..>2422)
                     /locus_tag="IV041_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8383562.1"
                     /translation="EMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGETAEAFHL
                     SHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDESQFNLE
                     KFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          696 a          413 c          364 g          949 t
ORIGIN      
        1 gatacagggg tttgttttgt gtttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagca ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagcg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct
     1201 ttctctgata caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac
     1261 acgcttgctt tggacggtgg gcgtgttttt tatttattat acagttgctt taaaaatagc
     1321 tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa
     1381 cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc
     1441 gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac
     1501 agggatatct gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg
     1561 ttggttttct tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc
     1621 agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt
     1681 agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt
     1741 attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa
     1801 tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg
     1861 acccataact ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat
     1921 ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt ttgctgaaat
     1981 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
     2041 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
     2101 atattccata aatttttcga ggttaaactg agattcatca gtcagttcgt tctcaatata
     2161 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
     2221 agttgtagaa gtatcgtaca aaactgaaaa ttcaaaatga gaggcgctga aatgtgcaag
     2281 ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat tcaaaaaaat
     2341 gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa ctactttcaa
     2401 attgtattcg ctcaacattt ct
//
LOCUS       DACDCV010000183         2408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDCV010000183 DACDCV010000000
VERSION     DACDCV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.190"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV041_002837"
     CDS             5..1150
                     /locus_tag="IV041_002837"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8383563.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV041_002838"
     CDS             1321..1779
                     /locus_tag="IV041_002838"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8383564.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IV041_002839"
     CDS             1938..2408
                     /locus_tag="IV041_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8383565.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          803 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaa
//
LOCUS       DACDCV010000184         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDCV010000184 DACDCV010000000
VERSION     DACDCV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.191"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV041_002840"
     CDS             207..2069
                     /locus_tag="IV041_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8383566.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IV041_002841"
     CDS             2069..>2400
                     /locus_tag="IV041_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8383567.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDCV010000185         2356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDCV010000185 DACDCV010000000
VERSION     DACDCV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.192"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..887
                     /locus_tag="IV041_002842"
     CDS             <1..887
                     /locus_tag="IV041_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383568.1"
                     /translation="IGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEV
                     WLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSY
                     NFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFE
                     GGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSG
                     DFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKT
                     MKNILKNVKIAESLDDIL"
     gene            884..2305
                     /locus_tag="IV041_002843"
     CDS             884..2305
                     /locus_tag="IV041_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8383569.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
BASE COUNT          841 a          269 c          417 g          829 t
ORIGIN      
        1 atattgggta ttttcaaaag attaaggact gtcttcagat ttcttgtggt aaatcaaatc
       61 gatatgccta ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt atttgttttt
      121 caatgggaga ggtttggttg gaaggaatat atgcttgctt atatcgaaat aataaaaatg
      181 tacaggtatc aattttagaa gaaggtattt taagctatgg agttgacatt attccaacag
      241 caagaacaaa agctatcgca gtactcaatt acttaaaaaa aaggactcca ctatataaca
      301 aaagctataa tttttattgt ttttttccta atatatatac aggaagagag aatacaatac
      361 aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat atttttaacc
      421 ctatgataga tatttataaa gaaaaaaaat atatattttt tacgtctgtt tatgattttg
      481 agggcggaga acctgtcgga gaatatgaat tagtatgcaa aatagcagac ttagtaggaa
      541 aagataatct aattgttaag acgcatccta gagatgtaag aacaatttat aaagataatg
      601 gatttatcga ggataaaaat tcatcaatac cttgggaggc gattcaattg tcaggcgatt
      661 ttaacgataa aatatttatg acaattaatt caggaagtgt tatttcaggt agtacaatgt
      721 ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg taacatagaa ggaaatccgt
      781 cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg atgaaaaaca
      841 ttttaaagaa tgtgaaaata gcagaaagct tagatgatat actatgagca aaactaaata
      901 tttaattaaa aatatgggaa tattaacaat aagtaatttt tcttcaaaaa ttttagtttt
      961 tttgttagtc cctttatata caagtgtatt atccacaaca gaatatggca catatgattt
     1021 gacaatttca acagccacat tattgtttcc aattttaacc ttaaacattg tagatgctgt
     1081 gatgcgcttt ttaatggata aagataaaaa taaaaaaaat atagcatcta tagcgattaa
     1141 agttatattt ttaagcgtga tagtctttgg atccgtaatg tatttgttaa atattacaaa
     1201 attatggcaa gatgtttatg gattagaaaa atatattttt ttgtattata tatcctatgt
     1261 atggaatcaa tttctaatac aatttgctaa agggttagaa aaagttaaag ctatgggcat
     1321 agcaggtatt atcagcacaa ttgtaacaat aattgccaat attttcttct tgcttattct
     1381 gaaatgggga ttaattggtt ttttcattgc aaatatttta tcacagatta tttcagctac
     1441 ttatctgtca gtattagtga gaatatggga atatatcgat tttgaggcta aagatcctca
     1501 gctaaagaga gaaatgctaa attactcgat tccattaatt gcaactgttg ttggatggtg
     1561 gattaatagt acagctgata ggtatgtggt ttcctttata gtaggtgtct cggcgaatgg
     1621 cttattgtct gtggcatata aaatacctca aataattaat actttgcagg gaatatttat
     1681 acaggcctgg caaatttctg caattaaaga atatggagat aatgagactg caaaatttta
     1741 tggaaataca tttgttatga ttaatttgtt aatgtgcata acctgttcaa gtcttatcat
     1801 attaactaga ccgttttcac atattcttta tgcgaaagac ttttatgaag cgtggaaata
     1861 tgttccattt ttattaatag ctaatgtttt aaattgtgca tcaggtctgt taggacctat
     1921 cttatcagca aaaaaagatt ctaaatccat gatgtggtct gcaatcatag gggcattagt
     1981 taatgtgatt atgagtgtag tccttgttta taagataggg gttcaaggtg ccacaatagc
     2041 cactgttata tgttcatatg taatatatgt tgttcgtaaa cgagctgtgg gaaaagatat
     2101 ttttataaag cattatcata ttgttgtgga atcttgggga ttattgtttg ttcaggcaat
     2161 catagaaata tattttaaaa attatgtaat agaaattata attatgatta ttttaattat
     2221 tataaatatc agttatatta agcagttgtg ttatagagga agaaacatta tttcaatgat
     2281 taagagtcga gagcacaaac ggtagacgat gttattttta taaaaagata taatttatta
     2341 atggtcgata attatg
//
LOCUS       DACDCV010000186         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDCV010000186 DACDCV010000000
VERSION     DACDCV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.193"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV041_002844"
     CDS             <1..378
                     /locus_tag="IV041_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8383570.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV041_002845"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV041_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8383571.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IV041_002846"
     CDS             1859..2026
                     /locus_tag="IV041_002846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383572.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IV041_002847"
     CDS             2080..>2232
                     /locus_tag="IV041_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8383573.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDCV010000187         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDCV010000187 DACDCV010000000
VERSION     DACDCV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.194"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..1440
                     /locus_tag="IV041_002848"
     CDS             13..1440
                     /locus_tag="IV041_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8383574.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1532..1897)
                     /locus_tag="IV041_002849"
     CDS             complement(1532..1897)
                     /locus_tag="IV041_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8383575.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1894..>2211)
                     /locus_tag="IV041_002850"
     CDS             complement(1894..>2211)
                     /locus_tag="IV041_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8383576.1"
                     /translation="LNICSIGILIVGIVLVLFKLFDWSHVKKNYAERNTRNADTRKLL
                     ASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVISYFLNQEIEDKEKQRRG
                     EVG"
BASE COUNT          727 a          421 c          370 g          693 t
ORIGIN      
        1 ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca gctgttttta
       61 tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat
      121 gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacag
      181 atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct
      241 cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt
      301 tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc
      361 agcaaagctt attcattaaa aaaacaagta ttagcttact ttaatgactg cgggatcgaa
      421 atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg
      481 tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc
      541 gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa
      601 gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca
      661 atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca
      721 gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg
      781 acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt
      841 caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc
      901 tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg tgcgctgatt
      961 catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa attttatctt
     1021 ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc
     1081 agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa
     1141 ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg
     1201 gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa
     1261 gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca
     1321 aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt
     1381 attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa
     1441 ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa
     1501 caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct aggggtccga
     1561 tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga
     1621 aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt
     1681 ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc tcgcttactc
     1741 ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac
     1801 aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcga
     1861 aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt
     1921 ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat
     1981 aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc
     2041 aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc
     2101 attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa
     2161 tagaacaaga acgatcccga caattaagat accgatagaa caaatattta a
//
LOCUS       DACDCV010000188         2201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDCV010000188 DACDCV010000000
VERSION     DACDCV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.195"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1404
                     /locus_tag="IV041_002851"
     CDS             4..1404
                     /locus_tag="IV041_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8383577.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
     gene            1391..>2201
                     /locus_tag="IV041_002852"
     CDS             1391..>2201
                     /locus_tag="IV041_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383578.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSL"
BASE COUNT          733 a          324 c          379 g          765 t
ORIGIN      
        1 tatatttggt tattaaatat ttgtacgatt ctgattagtt tttattatgg agaaaagttt
       61 tttaaaaaga tagatgctgc ttttttattt agttgtttat atacttttgc aacttatcga
      121 acagttgata tctattatcg atccgcaatt gcagaagcaa tcgctcttac gattatccca
      181 gctgttttat attatgctta tggattgata ttcaatagag aaaaaaaagt catcgcttta
      241 tctgtcagta taagtctact tgtgtatact catgtcttaa ctacactatt gtgtagtgca
      301 ttgattgctt gtatgattat agttcgtttg tttttaagca agcaaataaa aaacgatata
      361 aaatttgtgg tcgtgaattt agtaaaggct gtttttttga cttgtgcatt aactagctat
      421 ttttggtatc ctatgttgca gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca
      481 gatttgcagc tagaagcact ttctatttca gatagtatca ttcaagcgtt aaataacgat
      541 attacaacat acactatggg gttattagga ttggttgcgt tgatttttcc tttattcata
      601 ctgaaagaaa tggagaaaaa agaaaaaatc atctatgttg gcgtagtttc tacatgggta
      661 tgcgtaacta atttatttcc atggtttttg tttcaaaaaa caccgataag cttactccag
      721 tttccttggc gtatcttagg tatccaaatc tttttttctt caatgatcat aacgcttgtt
      781 tttatgaaaa agactatgaa taaaaaatat tcatggttaa tcattggcgc tactgtggtg
      841 tttctactcg tcacgtctat agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa
      901 cacggtcata ttgttatcaa tgaagaaaca ctcccacgtt atacaacatc agatatggga
      961 ggattatacg attacgcccc aacagaagcg ataaaagaaa ggcctcactt agaaaaacat
     1021 gaagtaaaag taggagaatc gtgggaaaaa gggaagtata aagctgcaga taataacatt
     1081 acttacacag tggcaagcaa caaaaagcaa aaagtgacag taccagtata tgcttattta
     1141 ggtacacagg taaaagtaaa cggtaaagtc gtaaatgact cttataaaaa acatggctta
     1201 gtgaatgtgg atgtaaatgc tggagaaaat aagatagaaa ttacaacaaa atatactcca
     1261 actgctctca tggctttatg gatcagtatt ctgacttatt gtttagtggt gtattcaagt
     1321 tttaaacgat ttgcacctaa taagttgaaa gattctcgat atgtgactaa aaaggagata
     1381 aacaagaaga atgaaaataa ataaaaaagt agtaatattg agtggattaa ttatagttag
     1441 tagtatatat tctttcatct ttttcaaaaa tacacagagt atttatacca ctggagatct
     1501 ttcttttcac ttatctcgaa tcaaggggtt atcgactatt tttaccagtc cgattaatta
     1561 tgagacattc aattatactg gatatggtgt aaattatttt tatccctttc tgactttttt
     1621 tccagctgta atgttatatt ggatgacaaa aaatttgatt gtaagctata ttatttacgt
     1681 ttggttattg aacttgtgta caacactcgt agcttatcat tatggcgagc ggtttttaaa
     1741 acaaaaaaaa gcggcatttt tatttagttg tctctatatt ttctctgctt atcggacagt
     1801 agacatctac tatcgttccg caattgccga agctatcgca ataacacttg ttatccctgt
     1861 attattttat gcttatcaaa tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct
     1921 agcattgagt atgagtttgc tagtgtacag ccatgtacta tcaacattaa tgagtacagc
     1981 attaattatc atttttattt ttattcgttt ggtttctaaa ggtttcaaga atgcagattt
     2041 tattgctata tttaaaaaat tatttagtgc ggcaggaatg acattggttc tgacgagtgc
     2101 tttttggtat ccgatgtttg aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac
     2161 aaatttatat gctcatgcat ctaatgtgtt cgatagtctg a
//
LOCUS       DACDCV010000189         2154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDCV010000189 DACDCV010000000
VERSION     DACDCV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.196"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IV041_002853"
     CDS             complement(<1..153)
                     /locus_tag="IV041_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8383579.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="IV041_002854"
     CDS             complement(475..696)
                     /locus_tag="IV041_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383580.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="IV041_002855"
     CDS             complement(794..1039)
                     /locus_tag="IV041_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383581.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="IV041_002856"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="IV041_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          716 a          382 c          311 g          745 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttac
//
LOCUS       DACDCV010000190         2152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDCV010000190 DACDCV010000000
VERSION     DACDCV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.197"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2090
                     /gene="acm"
                     /locus_tag="IV041_002857"
     CDS             <1..2090
                     /gene="acm"
                     /locus_tag="IV041_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8383582.1"
                     /translation="YADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQPG
                     DYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEGW
                     GQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPEDT
                     KHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQQ
                     FLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAWFK
                     EFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRADE
                     QPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINEND
                     TEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRLDN
                     GITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVISV
                     SGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQEI
                     IYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNLF
                     SQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLLIVT
                     ICAGIFLQKYKKV"
BASE COUNT          851 a          344 c          416 g          541 t
ORIGIN      
        1 tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa
       61 cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa
      121 atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg
      181 aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg
      241 tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag
      301 aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata
      361 cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag
      421 cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata
      481 ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag
      541 ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta
      601 atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt
      661 tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa
      721 ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag
      781 aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata
      841 aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg
      901 gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga
      961 ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac
     1021 agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag
     1081 taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa
     1141 gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta
     1201 tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca
     1261 ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag
     1321 ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata
     1381 tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa
     1441 acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa
     1501 atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc
     1561 aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc
     1621 aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat
     1681 acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta
     1741 cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta
     1801 taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa
     1861 ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac
     1921 aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa
     1981 gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga
     2041 tagtaactat ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata
     2101 atattttttg ttaaatatga ctgaaatcaa aagaataaaa ttccgattta ta
//
LOCUS       DACDCV010000191         2151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDCV010000191 DACDCV010000000
VERSION     DACDCV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.198"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..81
                     /locus_tag="IV041_002858"
     CDS             <1..81
                     /locus_tag="IV041_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP8383583.1"
                     /translation="PDDAQRLSENLKIMQKELQKIWQQLK"
     gene            63..977
                     /locus_tag="IV041_002859"
     CDS             63..977
                     /locus_tag="IV041_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8383584.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1222..1911
                     /locus_tag="IV041_002860"
     CDS             1222..1911
                     /locus_tag="IV041_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383585.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2032..2118)
                     /locus_tag="IV041_002861"
     CDS             complement(2032..2118)
                     /locus_tag="IV041_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8383586.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
BASE COUNT          843 a          407 c          392 g          509 t
ORIGIN      
        1 cctgatgatg ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
       61 atatggcaac agttaaagta agtcgaacca cttcatgtag ccgagcaatc aattacgcag
      121 aaccacgagc aaccgtcaaa acaggaataa actgtgacat tgattatgcc aaaagtgaaa
      181 tgaaacaaat acgcacgctt tatggaaaag acgatcgtgt acaagcacac ttattgattc
      241 agtcctttcg accaggagaa attaccgctg aaaaagccaa tcaactaggg gcagagtatg
      301 cgaaaaaaat agcccctaac caccaaatcg cgatttacac acatacagac aaagaccata
      361 ttcataatca cattgtcatt aactccgtta atcttgaaac tgggaaaaaa tttcaagccc
      421 atgggcaagc atttttaaac aaatgttatg acctaaatga cgagatttgt cttgaacatg
      481 acttaacaat cacggaacga ggaaagaaaa ccgaaaaacg aaccatgtct gaaattaaat
      541 taaaagaaaa aaatgaaccg gtttggaaag acgaaattcg gtttgcgatt gatcaaacaa
      601 tgaaaaacaa aaaaactcga acgtataccc aattttgtga ttcacttaaa acattcggca
      661 ttcattgctt gaatcgcgga aaaaatttta cttacgaatt ggtccacaaa aagaaaaaag
      721 tacgatccaa caaactagga aaggattacg aaaaggagac gattttacat gagctggaca
      781 gacgagaacg aacctacaat caacaacgaa ccaacgagct acgaagagct cgagctagca
      841 ttcgcgaaac tgcagaagaa gtacgaagag aacaacgaac ttttacgcct gaacaatcgg
      901 aaattagacc gactcgaaga aaccatgaac aacacgttga acgaggaaca gaccaccaac
      961 aagaattatc gctctaaaac gttaaaagac atcgaaaacg aaataaagag acaacgtgcc
     1021 tttcttgcgc cttataccca agaaaggata caaactacga tggattcaca agtcaaaaaa
     1081 ctccaccaag cgctattaga ccagcgaaac caagaacaaa aaaaggcttt aattcaacga
     1141 tcgatggata ttcttgcccc cttatgttgc ttagtactaa cattattagc cttttggttg
     1201 gtatggggag aataaacaga catggcattc tatttaccct atctactcat ttctgccagt
     1261 attttaggaa ttatttggct tagctataag gcttatcaaa caagatattc attaaaaggc
     1321 tctaaaattc gatttaagcg atttttattg gtcggggtta tatttgcctt atcaatcgtt
     1381 ttaagtggtt tattcggcgt tttagagggt aataaacgtg tttcaagaag cattttatta
     1441 ggggatatca ctcaaaagta tgaagcagca tgtgataaaa agaaaaaaga gcaagcatta
     1501 gcacaaaaaa tggaggaatt taccgcttgt tatgaggaca tgaacaatat ttttgtggat
     1561 caagagaaac gtttaacaga taaaaacatg aaaaaactca ctcgttttta tcaaaactta
     1621 ccagaaaaac agcaaaaaga ggtacaagaa aaatatgagc aagtaaaaaa agacgtgcaa
     1681 tattttaaag atacgcagac agaagaagct tgttgtaact tatttagcga tacaatccct
     1741 ttttcaacca gtgaacaaga acgaaaagaa cggcaacaat cggtcacata cgaacgttat
     1801 gaaacactgc ttcagcaagc aacaaacata caaaatccaa cgaaaaaaga aacggcatta
     1861 aattatctag aaagtgtcaa ggaatggttg gatcagcaac aacaaaattg aatttgatta
     1921 gcaatcatgt taaactgaac ctgcaaatga tcaaacatta gatacacgaa agccccagag
     1981 aggtcggatt ctctggggct ttttttcagg ttgatgtcgc ctttattgat tttacttctt
     2041 gtttcggtta ttcaaccaat aactgaataa agagacacaa acttctacaa gaagggggca
     2101 aatgaaccgt tcaaacatta gatacacctc cttccagtcg caagactgtc g
//
LOCUS       DACDCV010000192         2105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDCV010000192 DACDCV010000000
VERSION     DACDCV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.199"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1553
                     /locus_tag="IV041_002862"
     CDS             <1..1553
                     /locus_tag="IV041_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8383587.1"
                     /translation="LNEAHEIIIYERGENISYASCALPYYLGGVITDRDSLIERTPEI
                     LKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIKTDYDKLIISSGARPDYPDIPG
                     VFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAGVMGLELAENFKHRGLDVTLID
                     QLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEIRDKGREIVLSDGSVLSADMLI
                     FAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPDIYAIGDIIETTSVVTGQPIQS
                     MLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFDHTASYAGMTEHELKASGITNY
                     KTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQAFGEKGVDKRMGELSVAITGN
                     LTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTNIVETIKASDIPENDLKEAFFL
                     DIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKVYITFRRGLNTYTSARILAGLG
                     IKAILIEE"
BASE COUNT          667 a          374 c          415 g          649 t
ORIGIN      
        1 gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa
       61 gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac
      121 gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa
      181 cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa
      241 taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata
      301 ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc
      361 gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg
      421 ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg
      481 atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca
      541 aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata
      601 aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg
      661 ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata
      721 caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg
      781 gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca
      841 gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct
      901 atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg
      961 gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca
     1021 ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt
     1081 ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata
     1141 aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg
     1201 atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg
     1261 gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac
     1321 ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt
     1381 ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg
     1441 aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt
     1501 ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg
     1561 accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag
     1621 tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa gaaattgtgc
     1681 attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg cgcaatttaa
     1741 ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc
     1801 catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat taatctatca
     1861 gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct
     1921 ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt
     1981 ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc
     2041 cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat
     2101 ttaac
//
LOCUS       DACDCV010000193         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDCV010000193 DACDCV010000000
VERSION     DACDCV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.200"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IV041_002863"
     CDS             169..1539
                     /locus_tag="IV041_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8383588.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IV041_002864"
     CDS             1560..1844
                     /locus_tag="IV041_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383589.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDCV010000194         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDCV010000194 DACDCV010000000
VERSION     DACDCV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.201"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IV041_002865"
     CDS             complement(<1..337)
                     /locus_tag="IV041_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8383590.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IV041_002866"
     CDS             complement(404..559)
                     /locus_tag="IV041_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8383591.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IV041_002867"
     CDS             1176..1397
                     /locus_tag="IV041_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383592.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IV041_002868"
     CDS             1398..1655
                     /locus_tag="IV041_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383593.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="IV041_002869"
     CDS             complement(1791..>2058)
                     /locus_tag="IV041_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8383594.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DACDCV010000195         2024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDCV010000195 DACDCV010000000
VERSION     DACDCV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.202"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..538
                     /locus_tag="IV041_002870"
     CDS             50..538
                     /locus_tag="IV041_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8383595.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            525..1829
                     /locus_tag="IV041_002871"
                     /pseudo
     CDS             525..1829
                     /locus_tag="IV041_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          792 a          200 c          324 g          708 t
ORIGIN      
        1 gaaaacatga gcaatagaat tgtactagta ggataaggat ggatggaaaa tgaaaaaaac
       61 tatttttgtt ttagcgattt ttatatgttt tgggctactt ttggtaggct acttgttgtt
      121 taattctaat gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa
      181 ggataaaaaa aaagaatctc ttgcctttat aatgtttact caagaagagt gtgagtattg
      241 tatagcaata aatccaatca tagatagtgt aaataaggaa actttgaata agataaagaa
      301 atatgatata tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac
      361 tattgttgca tatagtaaga ataaagaagt ggggagaatt gacaatgaat tagataattt
      421 gtatcgaaaa aagcaagatg gttcaattgt aatagacaag aaaaaaacaa agacattgct
      481 tatagattgg atgaaaaaaa tggataaaca aggagaatat ttgtatggag aagattaaac
      541 agacatttcc gttaattaat aaatattgga ataagaaaaa aatttttata ttttttatta
      601 cgtttctgct gtcatcttta ttttctctaa ttttcccgta tctatttagt aatgttattg
      661 atttatattt agagaatgag ttatcacaga atattgtgcg aatagtaacg attcttatat
      721 ttcagttatt tttagtctta attaaagaag tgtatggata tataaaaact atttatgcat
      781 ttgaagttga tttatcaatg aaaattgatt ttcttaaggt tttacaaaaa aaaaatcttt
      841 ttttttcgca aacgaagcat tcaggagaaa ttcaatttag gatgttttcg gatatttcta
      901 ctatcgtaaa tactatcttt attttttctg ttgaattacc tgtgataggc gttttagttc
      961 ttgtagcggt atttattata agtaaagtga atcttatttt tctgattttt tcactaataa
     1021 tgatgggagc tatggtgtta cagtataatt tatttcaatt accgcttaag aaaacaatcg
     1081 agaatacaaa aagtgttaat caaagtatta caggagaatt gaatgcacat tttgatcaat
     1141 tagaaattat aagcgcatat aattttgaga atatctctat tttaaaattc gattctagat
     1201 ttaaagaatt atttaatgtt ttcaaaaaac aaaataaatt actaacactg ggaagctgtt
     1261 tggcacaggt tattttccaa ttatggattg gaggaatatt tcttattgat gttattttat
     1321 tagctaaagg aacaataagt attactaatt ttgtaatagt atttatgtac gctaacatgg
     1381 tccctacttt atggactact ttaattagta ttatttggaa atggcaagac tattcagtgt
     1441 cattaaatag atattacgaa tattattcaa ataacaacaa agtggctggc acaaaaaaaa
     1501 taaataaaat aaattctatt actggtcaat cattaaattt ttcatataac aaaagaaagg
     1561 tattaagcaa tataagtttt tcaactgaaa tgaataaact attcatgata tcaggagaaa
     1621 atggaacagg gaaaacaaca ctgatgaaat tatttactaa acagttgccg gttagtaata
     1681 atcaattatt cgtcaacggg atagatatta atatgattga taacgaatca ttaagaaggt
     1741 taatttcaat cgcaacacaa ataccatata taattaatag ctccgtgaag gagaatattt
     1801 taatggggac taaaaactat gataaaaaaa aattcagtaa attaatagaa ctattgggaa
     1861 taagtaaatt tatgacgaaa ttaggtatta ctttggacga caatatttat gatgaaaaaa
     1921 tattattatc tggaggagaa aaacaaaaaa taagtttagc tagggcgctt attaaatcag
     1981 cttcagttat tatattagat gaaccattta gaaatttaga taat
//
LOCUS       DACDCV010000196         2005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDCV010000196 DACDCV010000000
VERSION     DACDCV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.203"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..1641
                     /locus_tag="IV041_002872"
                     /pseudo
     CDS             6..1641
                     /locus_tag="IV041_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          655 a          306 c          357 g          687 t
ORIGIN      
        1 aagtaatgca agtaaagaaa aaaataatat tgtccattct cctagcaatt attgctggac
       61 tttattcaat caattatttt agaaatgttc atactatttc tacatcgggg gatattgcct
      121 tccattttgc tagagtcaaa gggttatctt caattttttc tggtccaatc aattttacga
      181 cgtttaatca ttatgggagc ggagtgaatt atttttatcc ctacttgacc ttttttccgg
      241 cagtcatttt ttattggata agtaataatt taatcgttag ttatatctta tatgtttggc
      301 tattgaatgt ttgtactatc atgctgatgt ttcattatgg actaaaattt ttaaaaagaa
      361 tcgatgcggc ttttatattt agctgtttat atacatttta tgggtaccgt acgattgata
      421 tttatcatcg ttctgctatt gctgaagcga ttgcacttac ggttataccg atagttatgt
      481 attatgctta cgcactgatt tatgagaaaa agccatgtgc aattccatta gcagtcagta
      541 tgagcttact catttataca catgttcttt ccacttttat gagtgtaata gccgttggct
      601 tactagtctt aggcggcttt gtagtaagtg aaacaagaaa aaaagaaagc atccggttga
      661 ttgtggagtt gtcaaaggca gtaggaatga cgattctttt aacagcatac ttttggtatc
      721 cgatgttccg acaaatcatg gttcaatcga ttaatgagcc agataaaacg accttacaac
      781 accaagcatt aaatgtctcg gatagtttga ttggtgcgat gaataatgac ctgacaacct
      841 ttacggtagg aatcattggt attatttctt tgatctgtcc actcttcttt tttaaaggat
      901 ttaatcgcaa agaaaaagtg atttacctta gtgcggtagt ctcttggctc ttatccacca
      961 aattttttcc atggtcttta ttacaaaata cacccattca aatgattcaa tttccttggc
     1021 gaatcttagg ctttcaagta ctgtttggct cgttgatttt atcgttcgtt ttcatgaaga
     1081 gcacatcagc taagcaaaga aaagtagtta atataacggc aattgtttta ttactgttgg
     1141 ctgtatctgc tgcaacaaaa gcaaactatg aacataaagt tgtcactcag agagatcact
     1201 tggtaatgaa tcaagaaaat cttattcgtt acacgacact ccttcccgga ggactttatg
     1261 actatgcacc cacggaagca ttacaataca aagaacattt gcagaatcat gaagtaagaa
     1321 ttggcaagga atggcatact atgccttatc aagtgatgga ttcgaaaata gtctatacag
     1381 tacatgaatc taaaggacaa aaaataacgc ttcctgtatt tgattattgg ggaacagtcg
     1441 caaaagtaaa tggacaaaaa agagagattg ataataggac tagtgtcgat tgaaggaaca
     1501 caaggagaaa caatcattga agtttcttcc atttatcctc caactatgaa gttagctttt
     1561 ttaattagtt tatgtagtta cctctttctt ttaattagtt ttcttatcag gaagttacaa
     1621 catataaaaa taatgatctg attacatttt tttcaatatt agcttactag agaataagaa
     1681 aaagtaagta ataaaccaaa tttaaacttt atattataat aatgaagctt aatcttcttt
     1741 atagagaatt gggctatttt taatggcaaa gaggggaaga agatgtttca aaacaaagga
     1801 caacgctatg ttgtaaaagg agtaaaggaa tcttactgga atcaaaaatc tttattgaga
     1861 aaattatctt tgaatttaag cacaaagaaa tgaaggtaca ctagatagca atgaagatat
     1921 ctttgtcatt aaacgtcgct attttacaca agtaagtgat gatttgattc atgtcttttc
     1981 caatgagtgg tttgtttctg agaaa
//
LOCUS       DACDCV010000197         1983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDCV010000197 DACDCV010000000
VERSION     DACDCV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.205"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="IV041_002873"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="IV041_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8383596.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="IV041_002874"
     CDS             853..1041
                     /locus_tag="IV041_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8383597.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="IV041_002875"
     CDS             1054..1611
                     /locus_tag="IV041_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8383598.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="IV041_002876"
     CDS             1643..1885
                     /locus_tag="IV041_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8383599.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          305 c          406 g          548 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 att
//
LOCUS       DACDCV010000198         1951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDCV010000198 DACDCV010000000
VERSION     DACDCV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.206"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            160..513
                     /locus_tag="IV041_002877"
     CDS             160..513
                     /locus_tag="IV041_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383600.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(571..759)
                     /locus_tag="IV041_002878"
     CDS             complement(571..759)
                     /locus_tag="IV041_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383601.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(857..1897)
                     /locus_tag="IV041_002879"
     CDS             complement(857..1897)
                     /locus_tag="IV041_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8383602.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          643 a          353 c          312 g          643 t
ORIGIN      
        1 tataggtaaa taaaatcagc gacctttcta tttgaaattg gattcatcgc attaataaat
       61 tccacagtgt tctataggaa ctcataaact atataacatg tttttctata cctgtttatg
      121 gttatatagg aacagtaaaa tgtatagagg tgatttagta tgcgtaaaaa agaagataaa
      181 tacgatttta gagccgttgg tttagcgatt aaagaggctc gaatgaaacg gggtctcact
      241 cgtgaacaag tgggaacaat gattgaaatt gacccacgtt atttaacaaa tatagaaaac
      301 aaaggacaac acccaagtac acaagtgctg tatgatcttg tatcattact ccatgtatct
      361 attgatgaat ttttcttacc tacagataat ttgattaaaa gcactcgaag gttacaggta
      421 gaaaaataca tggatagctt tacagacaaa gaactatcct taatggaagc actagcaaaa
      481 ggtatcaatg aagcaagaaa tattgaaggc tagtcagtaa aattcagata aacaaaaaga
      541 gccgataaga tgagagtttc atttctcaaa ttatcggctc tgcgtcttgg cgtctggctc
      601 tttgattgtt attatattca ttttttgaac attaattaaa gtttcgttct tacatttggg
      661 gcagtataaa gggaaatttt taagtatagt atccactcgt attcgtagtc ttgttttatt
      721 tccacaaata ggacacaata tccacttgta gtttataata acaatctcct cctttccact
      781 ttaattcaaa tctatattaa agaatatttc atcttattta ataagaaacc atatttatat
      841 aacaacataa aacgcactaa gttattttat tgaacatata tcttacttta tctatccgac
      901 tatttagacg acgggtctgg caaacaggtt cgccagtggt aacctgatat ccttttagct
      961 ctgctaaaca aacactaagc ccatttgtaa aaaaagttaa atcattgcga taatcttgaa
     1021 tacatcgagc aggaatttct ccaataataa tgacctcatt atttttcagt tgagtattta
     1081 cgatatttgc acaatatttg ggagcatcgt tatatgcccg tgaaagatat tcctgtggtg
     1141 cataaacttt aaaactaaga tatggctcta acaattctgt tccagctttt ctaaaggctt
     1201 gctccagtac aataggagta agcatccgaa aatctgctgg agtactaaca gggctatagt
     1261 ataaaccgta cttaaaacag attttacaat ccgtcacatt ccaaccatat aatccttgtt
     1321 cgcaaccata gcgtatccct tccataactg cattttgaaa tgattgattt aagtatccaa
     1381 gagaaaccga gctctcatac tgcattccac ttcccaacgg aagcggtgat acagataaac
     1441 caatggaagc ccagaaagga tttggcggca cttcgatgtg aatggtatat tctgcatttt
     1501 ttaacggtct ctccatataa atgactgtag gctcttttag ttctatctcc acatgatact
     1561 tttcttgcaa cagtgcacta atcacttcca tttgtacttt ccctaagaaa gaaagtataa
     1621 tttcatgtgt cgtagaatcc acgtaatatc gtagaagcgg atcactatct gagatttcca
     1681 aaagggcatc aagcaacatt tctctctgtt caggtttact cggttcaaca gttgtttgta
     1741 gtagagggtg cggattttca atcttttttc tctgtggcaa tagttttgta tctccaagaa
     1801 cactatttaa cttcaaaaac tcattttgca aaataacaat ttctccagaa taagctctat
     1861 caatcttaca taattcacca tttattgaag tatacatttc tgtaactttt attttttctt
     1921 tttctgatac tctaaccgaa tctcgtaaat g
//
LOCUS       DACDCV010000199         1920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDCV010000199 DACDCV010000000
VERSION     DACDCV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.207"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..>1920
                     /locus_tag="IV041_002880"
     CDS             62..>1920
                     /locus_tag="IV041_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8383603.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKP"
BASE COUNT          749 a          263 c          335 g          573 t
ORIGIN      
        1 attgatttga aataatcaag aatattagaa acttctacat taatcaagaa ctttcttctg
       61 tatgaaaata gagataataa ttgtacactt aagctgtatt caggaggtga agataatggg
      121 catcgaattt agcgaacggc aaatgaaaat actatacttt ttgattgaaa atgagcacac
      181 aacttacaaa gaattatcag aacaattata tgtatctacg aaaacaatta gcaaagatat
      241 tttaattttg aaagaaataa tccaaaaaat gccagatgta caaatgttgg ttcaacctaa
      301 aaaaggcgtt tcgcttattg gaaaaaaaga aacacttcga aatttattat ccagtcataa
      361 aatcaatatc gcagactctc cggaggaccg tctaacttat gtgtttgcta aattattgaa
      421 tactaatgat tctctaaaga ttcaaaatct tgctgacgaa ttatacgtga gcaggtcgac
      481 ggtagaaagc acaatgaaag atgttagaaa aagactaaac aagcatggga tgtccttagt
      541 ttcagatcga aatggcgtgc gcatcaaagc tgatgaaaaa agtaaaagaa ggttgatgtc
      601 agaagttgtg aactattatt ggggtggctt tacagcatct aacagtaaaa cagatctctt
      661 agacttgaat atcaatttca ttggtgaagc gaataatttc ttaaataagg acatattgaa
      721 aaaagtagcc gaattgctta atcgatacat gaataagaca caattgaaat ttacagatta
      781 tgaatttcaa actttagcta tccaccttgc tattgcgata gaaaggattg aaacaagcaa
      841 tttcttatca cgtagtaata attctgaaga agaaatctca aagaatacac ttattttaat
      901 caaaatgatt gaaaaagcat tttcaattat tgtgccaata gatgaacaag cttatgttga
      961 cattcacatt acttcgattg aaaaaaatgc tatgaatcac gacttagatc aaacgaagct
     1021 ttcctattta gatatgggtg ttgagctaca agagttagta agaattaaat taaatgaatt
     1081 ttatcctgat gaagaattga tcaataatct agtattacat ctcaatgcag cagttaaaag
     1141 actcaaattg ggagtaaata tctataaccc ttatacggac aaaatcaaat attcttttaa
     1201 aaggtcattt atgataagtg tagatttatt agaggaaatc gaagagagat tctgtataca
     1261 ttttaatgaa gatgaaatcg cttacgtaac actccatatt caatcacttt tggatcgtta
     1321 caaacctgac aaaacgaaag tgatattagt ttgtagtagc ggatatggaa catcaaaatt
     1381 attagagcaa agaataaata acggttttgc agatatggtt gagatagaag atgtactaag
     1441 catcaatgaa ctacaagata gaaaagtcac tgatgaactt gttataagca cgattccgat
     1501 tgaaaataca aattttcctg ttattgttgt tagtcctttg atgttagggg cagatgtcag
     1561 aaaagtaaaa cagtatctac agttaaatga acaaacagaa gatgcttttc taaatttatt
     1621 agataaacga ttttgctttt ttacccacca aataaaggat aatcaagtga acgttttaaa
     1681 tataattacc aaaaaattgg taaaagaaaa ttacgcgaaa gaagggatac tagagggagc
     1741 aatcaaaaga gaaaaactat cttcaacagc gatgaatgat tttgctatgc cgcatatcga
     1801 agtagatttt ataaaaaagc cagtaatctg tgtctacgtt aatccagaag gaattgattg
     1861 ggatgggaca cttgttcatt ctgttttctt ttttgcactt aatgaaaaag taaaacctga
//
LOCUS       DACDCV010000200         1887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDCV010000200 DACDCV010000000
VERSION     DACDCV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.209"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            502..834
                     /locus_tag="IV041_002881"
     CDS             502..834
                     /locus_tag="IV041_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8383604.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1453..1794
                     /locus_tag="IV041_002882"
     CDS             1453..1794
                     /locus_tag="IV041_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8383605.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          653 a          255 c          408 g          571 t
ORIGIN      
        1 tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt
       61 tcgttctgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg
      121 gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc
      181 ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga
      241 tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg
      301 atgaatctat gcctaagcac aatgttataa aaatgtccgt gtggctcttt gccgtatgac
      361 gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg
      421 aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa
      481 agaaattggg tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt
      541 cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa
      601 agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa
      661 atgatttatt acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa
      721 gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga
      781 aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat
      841 cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct
      901 aaaggaactt agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt
      961 cggggagtga cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa
     1021 atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca
     1081 agagggaatt tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac
     1141 taagggatct agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg
     1201 gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt
     1261 gattattgga tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca
     1321 taaacgagtc gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca
     1381 agtcctgaat aaagtgaaaa aattgatata aaacatagag aaaatgaaat ttacgtaatg
     1441 gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag
     1501 gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg cggaaattgt
     1561 aaaggattag atgttttagc attagacgag gatgatttat acgatcaaac attgactgat
     1621 aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg
     1681 acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt
     1741 gattatattg tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg
     1801 gaggaataaa ttttacagat aaaggcttag aaggagtcac gatgattaat aaaaaggtag
     1861 tattacctta ggcattgagt aaggaat
//
LOCUS       DACDCV010000201         1882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDCV010000201 DACDCV010000000
VERSION     DACDCV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.210"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            180..746
                     /locus_tag="IV041_002883"
     CDS             180..746
                     /locus_tag="IV041_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8383606.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            891..978
                     /locus_tag="IV041_002884"
     tRNA            891..978
                     /locus_tag="IV041_002884"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:923..925,aa:Ser,seq:cga)
     gene            1312..>1882
                     /locus_tag="IV041_002885"
     CDS             1312..>1882
                     /locus_tag="IV041_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8383607.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          663 a          282 c          361 g          576 t
ORIGIN      
        1 taaaaatgta agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt
       61 taaagaagac tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat
      121 gctaaaaata gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa
      181 tgagaagaaa agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa
      241 aggaagggtt taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac
      301 agccgattta tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta
      361 tctttgaaca actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg
      421 atctcggttt gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt
      481 acattgatga acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg
      541 aaatagttaa agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc
      601 atactggtac atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc
      661 atccaacaga agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat
      721 tagataatcc tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg
      781 ttggctgcaa actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta
      841 ttagaaatag gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg
      901 cagagtggta atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt
      961 caaatcctgt accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca
     1021 agtaataaga acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat
     1081 taccgcagat gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt
     1141 ttgtcacagc cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt
     1201 actatttttt ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat
     1261 actagatgta agataaaagt gtaaaaacga tttgttttag gaggggaaac gatgtgtacc
     1321 gcaatcactt atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct
     1381 tataatgaag tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa
     1441 aatttagata aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc
     1501 tattatgagg ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat
     1561 gctgattata aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca
     1621 tggatactag ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat
     1681 ctagtcaata tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct
     1741 gataaagaaa aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat
     1801 cctgtcggtg tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat
     1861 tatcgctcgc tatcaaatgg aa
//
LOCUS       DACDCV010000202         1841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDCV010000202 DACDCV010000000
VERSION     DACDCV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.211"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            166..978
                     /locus_tag="IV041_002886"
     CDS             166..978
                     /locus_tag="IV041_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8383608.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            975..1775
                     /locus_tag="IV041_002887"
     CDS             975..1775
                     /locus_tag="IV041_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8383609.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          569 a          285 c          405 g          582 t
ORIGIN      
        1 ttccgcttcc tctgttttta tttatatttc ttttatttaa aaattaaaaa aagaaaaaat
       61 tattcagttt ttaaataaaa aaacaatgat ttcataggaa aaacagttag atattgctat
      121 aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt
      181 ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca
      241 attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt
      301 ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa
      361 gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag
      421 cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc
      481 gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg
      541 ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga
      601 atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa
      661 gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg
      721 attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg
      781 atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt
      841 gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca
      901 atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag
      961 aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta
     1021 ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa
     1081 taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt
     1141 tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc
     1201 cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat
     1261 cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct
     1321 tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt
     1381 tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac
     1441 gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc
     1501 tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa
     1561 aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag
     1621 tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact
     1681 cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg
     1741 tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga
     1801 atttcggctc tttgtcaaat gatgttggta aagagaaatc t
//
LOCUS       DACDCV010000203         1822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDCV010000203 DACDCV010000000
VERSION     DACDCV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.212"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            41..379
                     /locus_tag="IV041_002888"
     CDS             41..379
                     /locus_tag="IV041_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8383610.1"
                     /translation="MRQKYGKKKVTASNEKWMNMDKETYEEMQTVEKQMLFDLTLYLK
                     EPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGMMYEADERFTAYYDERSGHGAAK
                     ALNEIIQKKA"
     gene            complement(519..1442)
                     /locus_tag="IV041_002889"
     CDS             complement(519..1442)
                     /locus_tag="IV041_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8383611.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1457..1729)
                     /locus_tag="IV041_002890"
     CDS             complement(1457..1729)
                     /locus_tag="IV041_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383612.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          583 a          374 c          301 g          564 t
ORIGIN      
        1 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
       61 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
      121 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
      181 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttat cttacagctg
      241 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
      301 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
      361 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
      421 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
      481 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
      541 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
      601 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
      661 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
      721 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
      781 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
      841 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
      901 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
      961 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
     1021 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
     1081 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
     1141 ttctgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
     1201 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
     1261 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
     1321 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
     1381 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
     1441 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
     1501 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
     1561 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
     1621 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
     1681 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
     1741 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
     1801 aaagaaaagt tttattcctc tt
//
LOCUS       DACDCV010000204         1795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDCV010000204 DACDCV010000000
VERSION     DACDCV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.213"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..193)
                     /locus_tag="IV041_002891"
     CDS             complement(<1..193)
                     /locus_tag="IV041_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383613.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREE"
     gene            925..1182
                     /locus_tag="IV041_002892"
     CDS             925..1182
                     /locus_tag="IV041_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8383614.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1153..1461)
                     /locus_tag="IV041_002893"
     CDS             complement(1153..1461)
                     /locus_tag="IV041_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383615.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1539..>1795
                     /locus_tag="IV041_002894"
     CDS             1539..>1795
                     /locus_tag="IV041_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8383616.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSA"
BASE COUNT          646 a          304 c          306 g          539 t
ORIGIN      
        1 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
       61 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
      121 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
      181 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
      241 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
      301 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
      361 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt
      421 agtcaacgca gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag
      481 aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa
      541 caagcaaaag tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc
      601 atagcagaga ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca
      661 tagataacag cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat
      721 tcgcctttag tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg
      781 atttcaaacg gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt
      841 cgtggtaata ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa
      901 tcatttatta aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa
      961 aaaatgatag atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa
     1021 ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg
     1081 aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac
     1141 gagcaagaat tactatttga aaccgatact tttaacagct gatgttttat agatgacagt
     1201 ttcatccgaa tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat
     1261 aagacctaca agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt
     1321 attcacgtaa atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc
     1381 atcgtatcca acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt
     1441 aattacaaat tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca
     1501 ctgataagga aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta
     1561 atttcgagca aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa
     1621 aagatgtagc agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg
     1681 acttggagga taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga
     1741 ttaacgagtc aggcctttac agtttaatct taaaatcaaa acttccctct gccaa
//
LOCUS       DACDCV010000205         1734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDCV010000205 DACDCV010000000
VERSION     DACDCV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.214"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(105..189)
                     /locus_tag="IV041_002895"
     tRNA            complement(105..189)
                     /locus_tag="IV041_002895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Leu,seq:caa)
     gene            complement(207..278)
                     /locus_tag="IV041_002896"
     tRNA            complement(207..278)
                     /locus_tag="IV041_002896"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Gln,seq:ttg)
     gene            complement(288..360)
                     /locus_tag="IV041_002897"
     tRNA            complement(288..360)
                     /locus_tag="IV041_002897"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:His,seq:gtg)
     gene            complement(422..506)
                     /locus_tag="IV041_002898"
     tRNA            complement(422..506)
                     /locus_tag="IV041_002898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:caa)
     gene            complement(527..597)
                     /locus_tag="IV041_002899"
     tRNA            complement(527..597)
                     /locus_tag="IV041_002899"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Cys,seq:gca)
     gene            complement(643..714)
                     /locus_tag="IV041_002900"
     tRNA            complement(643..714)
                     /locus_tag="IV041_002900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Gln,seq:ttg)
     gene            complement(726..798)
                     /locus_tag="IV041_002901"
     tRNA            complement(726..798)
                     /locus_tag="IV041_002901"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:His,seq:gtg)
     gene            complement(801..874)
                     /locus_tag="IV041_002902"
     tRNA            complement(801..874)
                     /locus_tag="IV041_002902"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Trp,seq:cca)
     gene            complement(881..961)
                     /locus_tag="IV041_002903"
     tRNA            complement(881..961)
                     /locus_tag="IV041_002903"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Tyr,seq:gta)
     gene            complement(963..1038)
                     /locus_tag="IV041_002904"
     tRNA            complement(963..1038)
                     /locus_tag="IV041_002904"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Phe,seq:gaa)
     gene            complement(1074..1146)
                     /locus_tag="IV041_002905"
     tRNA            complement(1074..1146)
                     /locus_tag="IV041_002905"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Asp,seq:gtc)
     gene            complement(1156..1228)
                     /locus_tag="IV041_002906"
     tRNA            complement(1156..1228)
                     /locus_tag="IV041_002906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1193..1195),aa:Val,seq:tac)
     gene            complement(1244..1315)
                     /locus_tag="IV041_002907"
     tRNA            complement(1244..1315)
                     /locus_tag="IV041_002907"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1280..1282),aa:Glu,seq:ttc)
     gene            complement(1330..1419)
                     /locus_tag="IV041_002908"
     tRNA            complement(1330..1419)
                     /locus_tag="IV041_002908"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1383..1385),aa:Ser,seq:gga)
     gene            complement(1459..1532)
                     /locus_tag="IV041_002909"
     tRNA            complement(1459..1532)
                     /locus_tag="IV041_002909"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1496..1498),aa:Asn,seq:gtt)
     gene            complement(1542..1657)
                     /gene="rrf"
                     /locus_tag="IV041_002910"
     rRNA            complement(1542..1657)
                     /gene="rrf"
                     /locus_tag="IV041_002910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          492 a          442 c          385 g          415 t
ORIGIN      
        1 ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata aaaacaaaaa
       61 aaatatagca actctctata agaaagttgc tatattgata attataccgg cggccggggt
      121 cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc
      181 cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg
      241 agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaagggc gaatgatggg
      301 aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc
      361 catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca
      421 ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc
      481 gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga
      541 tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt
      601 atactattat caatctataa aataaagaat aaattataga aactggggta gctggattcg
      661 aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata
      721 aaaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa
      781 ccacttcgcc acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt
      841 ggagaccgct gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa
      901 ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc
      961 catggtggcg cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac
     1021 caactgagct accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc
     1081 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
     1141 ggaaccagtt cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca
     1201 gatgctctcc cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc
     1261 gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta
     1321 aatttgctac ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga
     1381 tttccaatca tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac
     1441 tctttctcaa aatgagaact ccggcagtag gactcgaacc tacgacatca tgattaacag
     1501 tcatgcgcta ctaccaactg agctatgccg gaataacatc agcgtggcga cgtcctactc
     1561 tcacaagggg caacccctca ctacaatcgg cgctaagaag cttaacttct gtgttcggca
     1621 tggttacagg tgtatccttc tcgctatcgc caccacactg tggtgttatc ttttattgag
     1681 taaattttgt tcactcaaaa ctggatttga agtcacataa gtttttttcc gagt
//
LOCUS       DACDCV010000206         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDCV010000206 DACDCV010000000
VERSION     DACDCV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.215"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV041_002911"
     CDS             131..505
                     /locus_tag="IV041_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8383617.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV041_002912"
     CDS             complement(619..1248)
                     /locus_tag="IV041_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8383618.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV041_002913"
     tRNA            complement(1382..1454)
                     /locus_tag="IV041_002913"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACDCV010000207         1660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDCV010000207 DACDCV010000000
VERSION     DACDCV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.216"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..752
                     /locus_tag="IV041_002914"
     CDS             138..752
                     /locus_tag="IV041_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383619.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="IV041_002915"
     CDS             complement(776..1126)
                     /locus_tag="IV041_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8383620.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="IV041_002916"
     CDS             complement(1120..1383)
                     /locus_tag="IV041_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8383621.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          558 a          294 c          256 g          552 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa
//
LOCUS       DACDCV010000208         1596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDCV010000208 DACDCV010000000
VERSION     DACDCV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.218"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IV041_002917"
     CDS             complement(<1..626)
                     /locus_tag="IV041_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383622.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..1567
                     /locus_tag="IV041_002918"
     CDS             914..1567
                     /locus_tag="IV041_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8383623.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          572 a          224 c          299 g          501 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat gtaggatata aattggagag
     1561 aaaatgaaaa aattaaagat atttccaaaa atgttc
//
LOCUS       DACDCV010000209         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDCV010000209 DACDCV010000000
VERSION     DACDCV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.219"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV041_002919"
     CDS             <1..1350
                     /locus_tag="IV041_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8383624.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          626 a          242 c          337 g          381 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaa
//
LOCUS       DACDCV010000210         1573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDCV010000210 DACDCV010000000
VERSION     DACDCV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.220"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DACDCV010000211         1565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDCV010000211 DACDCV010000000
VERSION     DACDCV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.221"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1014
                     /locus_tag="IV041_002920"
     CDS             <1..1014
                     /locus_tag="IV041_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8383625.1"
                     /translation="LQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELT
                     DDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVT
                     QDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAF
                     CPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINY
                     IKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHS
                     LSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHD
                     ILI"
     gene            1070..1288
                     /locus_tag="IV041_002921"
     CDS             1070..1288
                     /locus_tag="IV041_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383626.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1359..>1565
                     /locus_tag="IV041_002922"
     CDS             1359..>1565
                     /locus_tag="IV041_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383627.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKF"
BASE COUNT          582 a          185 c          301 g          497 t
ORIGIN      
        1 cttcagatgg gtgaaattgt tagaggtcct agtcaaatat ctattgatgt tacatataaa
       61 tgtaatttta gatgtaaaca ttgttacaat gcgagtggtg gagacaattg ttttatggat
      121 aatgagttga ctgatgatga actgttagaa cttacaaaag atatatgtaa tttaaagcca
      181 tataatattt gtttctgtgg aggagaaact ttactgagaa aaaaaatagt tttatcttgt
      241 gccactttat taaaagaggc aggtattcct aatattgcta ttgttactaa tggatcatta
      301 gtaactcagg atgttgcaaa agaattattc aaagcaggta ttaatcgatt tcaagtaagt
      361 ttagatggaa aaaatgctag tagccatgaa gcacttagat tatatccaaa cagttttgaa
      421 aaggcaatta atgctatcaa attatttaga gaattgtccg aagaagtgga tgttgctttt
      481 tgtcctacta gttttaatta caatcaatta cgagaagtat atcaactagt aaatactctt
      541 aaagtgaagt ctttaagaat tcaaccattt atgcttagtg gtaggggaat gcaaaatagg
      601 gaaatagtgc caactgtttc tcaatataga aaactagttt ctgatattaa ctatattaaa
      661 agaaatgaag attataccaa gatagattgg ggagatccaa ttgatcatat catccgacac
      721 agaactttat tgaaagattt ttcgaatttt gtttctatta ggtcagacgg aggattaatg
      781 gcatcacctt acttgccaat tcaagttgga aatattaaat gtcacagtct ttctaaatat
      841 tggcgagcag gactttataa agttttttct ttagatttag ttcaagatat agctactcaa
      901 gtttcttcaa tacaagatat gagtaaagaa ttaaaaaaag gacatattaa atggcttaat
      961 gaagatatag aaattgatat tctcgatgaa tacaaacatg atattttaat ttaggtttat
     1021 cattggaaag tttagataaa caaagtactt ttagaaagga gggaaaaaaa tgatttctga
     1081 acctcaaatg aataatatgt atgaaagccc aactataatg ggagctggtg aagctgcgcc
     1141 aagagcttgt tcagcaatta tggtgtgtgt gttattagca tctgcaggag taggtatcca
     1201 agcaggagcg gtgtatactt atgctgtagt tgctacagta gctgtagctg cagccgcagt
     1261 ttggaaaaca gtaggattag ttggttagga attaatagga gactaactgt ctcctattga
     1321 ttccaatgat taagctaagg aggaatttta agaaaataat gaaatacaat aatttaaaac
     1381 cgaaattact taatgagaac atagaatata taagagaaga aaattatgga tatttaacca
     1441 tagttcccaa aaatggaact gaattgaaag ataacgtttt caatccaaca gtaagatata
     1501 tattggctaa ctgtactgga aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga
     1561 agttt
//
LOCUS       DACDCV010000212         1548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDCV010000212 DACDCV010000000
VERSION     DACDCV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.222"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..1508)
                     /locus_tag="IV041_002923"
     CDS             complement(27..1508)
                     /locus_tag="IV041_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8383628.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          490 a          212 c          270 g          576 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagac
//
LOCUS       DACDCV010000213         1540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDCV010000213 DACDCV010000000
VERSION     DACDCV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.223"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            309..1349
                     /locus_tag="IV041_002924"
     CDS             309..1349
                     /locus_tag="IV041_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8383629.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          613 a          201 c          235 g          491 t
ORIGIN      
        1 tttatatgca taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt
       61 atctttaatt ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa
      121 taatatttat ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt
      181 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
      241 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      301 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      361 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      421 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      481 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      541 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      601 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      661 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      721 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      781 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      841 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
      901 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
      961 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
     1021 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
     1081 agaaaaaagc tgaaatagaa agtggtattt cgatagtttt tgaagaactc gatagttatt
     1141 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1201 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1261 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgaa agctccccaa
     1321 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat
     1381 ggagtaacat taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa
     1441 taatagctat tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag
     1501 ccttttacgt cataagtgtt aaaagaaata attaatttca
//
LOCUS       DACDCV010000214         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDCV010000214 DACDCV010000000
VERSION     DACDCV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.224"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV041_002925"
     CDS             <1..395
                     /locus_tag="IV041_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383630.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IV041_002926"
     CDS             398..1147
                     /locus_tag="IV041_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8383631.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IV041_002927"
     CDS             1159..1428
                     /locus_tag="IV041_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383632.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT          607 a          253 c          304 g          366 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca
//
LOCUS       DACDCV010000215         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDCV010000215 DACDCV010000000
VERSION     DACDCV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.225"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1061)
                     /locus_tag="IV041_002928"
     CDS             complement(<1..1061)
                     /locus_tag="IV041_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383633.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
     gene            complement(1085..>1517)
                     /locus_tag="IV041_002929"
     CDS             complement(1085..>1517)
                     /locus_tag="IV041_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383634.1"
                     /translation="GKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPE
                     YYFADYGEAVTGDDGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFII
                     ETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          428 a          316 c          235 g          538 t
ORIGIN      
        1 gttgagcctt cttcgatttt gatgtcgtac aacttaaatc cgccatttat tttatccctg
       61 tcaacaaagc taatactcat ataaaatcga tctaaatttg tgatttgata attaacgtta
      121 gctgtacctt tgattgttat ttctttccct acatcatctg ttgtaatatt tgtactattt
      181 gcttccaata atatcttttc tcctggtgat gtacgataca ccaaacgtaa tttatcaata
      241 gctcctgtag ttccttcatc aaatcgaact ttcgcactca gagtataggt tttcccacta
      301 gcaagctgag gtgtattaat acgcgtaaac ataataatgc ttcctgtacc atcagaagta
      361 aaatgtagct tttcgttatc cgaagttagt gacccgtggt aaccaacgtt aaaatcgctc
      421 gattttagtt tggacattaa attcggctta ccgctataat catatccccc gaagtcgatg
      481 ctgttactgt acatcacctg caagttacct aatttagaaa tttcttcttt cagagcgtct
      541 aacttgtctt gtagtgtttt agcttgacca gttaaatcag taatctgttt atttaagctg
      601 tccactctac cttcgatttc agccataaaa gcatcaaaag tttcgttata cttttgaatc
      661 aactcttcca attgcgaaac gtattcatcg gcttggcctt gcgaaatgtc agacactcct
      721 agtgagaaaa aaatgatatc ttgcgttgtt aaaatttgat tgtcttttct atattctacg
      781 tagcagtgtt tataatatcc tgcttcactc ataaatgtgc catcaagaga aaacgtgact
      841 tcttcactag ttacactagt tgccacacta tctacgtaac ggttagatgg cgttgttcct
      901 tttaaagtaa atgttccgcc actcgtatcc atctgcaagc catttaaaaa tggtttaaca
      961 gtcactgtaa tccctttatc accttgacga gccataatag ctttggtgta gtttaattct
     1021 ttgctgaaat ctagagccaa attatataaa ctgctagcca tttatatacc tccttgtctt
     1081 cgttttaaaa acgtttttgg tcaagcactg tgctatcata tgctgtatcc tcttttaatc
     1141 taatatcttc ataccctaga cggtgtgcca ctaaattcca tctaactaat acgtttggct
     1201 tactagtttc aatgatgaaa tggtcaatat cttcatgagt aacagcacac aaaactagtt
     1261 ctgtaggtgt cacatgtgtc atatatcgac ttagatttac tgtctcagca aacatggggt
     1321 caatatcaac acgaacttta ccatcatcac ctgtaacggc ttccccataa tcagcgaaat
     1381 aatattctgg agtttcataa gcgtttaata gtcgttgtcc ataatgttct gttggtacgg
     1441 ttgagttttt agtacctcta acagtaaaat ctttatatac ttgtaccgtt gattgttcaa
     1501 acctagcaag tttccca
//
LOCUS       DACDCV010000216         1498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDCV010000216 DACDCV010000000
VERSION     DACDCV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.227"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IV041_002930"
     CDS             316..570
                     /locus_tag="IV041_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8383635.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IV041_002931"
     CDS             571..888
                     /locus_tag="IV041_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8383636.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          531 a          228 c          290 g          449 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatata
//
LOCUS       DACDCV010000217         1495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDCV010000217 DACDCV010000000
VERSION     DACDCV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.228"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..357
                     /locus_tag="IV041_002932"
     CDS             <1..357
                     /locus_tag="IV041_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383637.1"
                     /translation="LIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIS
                     TISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILL
                     PENWAELRKLVEYGII"
     gene            344..>1495
                     /locus_tag="IV041_002933"
     CDS             344..>1495
                     /locus_tag="IV041_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8383638.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          477 a          198 c          240 g          580 t
ORIGIN      
        1 ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa catattgaat
       61 tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac acttttgatt
      121 agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac gatcaactct
      181 attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga taaaaaagaa
      241 acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga agaaaaaatt
      301 ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat tatttaacac
      361 actttccaag tcaaaattag ttaagtccag tttctggtat actataggga atttttttat
      421 taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc aagcagaata
      481 tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg ttgggctaag
      541 cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa atgaatttat
      601 gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa tagggaacat
      661 atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta atttgatgtt
      721 attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta cgattaactt
      781 taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata ttgcaatctc
      841 tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag tagtcggcgg
      901 aacactgtca tttgtgttga taggtttagt aatttattta agaataatga taaagggtgc
      961 tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc cgctaatccc
     1021 acattcctta gcaaatatag ttttagcaca attcgataga attatgatta attcttattt
     1081 aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta ttctaagcgt
     1141 aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga tgagtaaaaa
     1201 taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta ctttgattac
     1261 gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga aagactatta
     1321 ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt ttttatatag
     1381 tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga ttggtacagt
     1441 agcaagtgct atagttaata tactattaaa catgattttc atacctaggt tagga
//
LOCUS       DACDCV010000218         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDCV010000218 DACDCV010000000
VERSION     DACDCV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.229"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(98..1228)
                     /locus_tag="IV041_002934"
     CDS             complement(98..1228)
                     /locus_tag="IV041_002934"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8383639.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          420 a          300 c          236 g          528 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaatccttt tactagcaaa tactagtaaa aggacgatta tactcgtggt accacctttt
     1321 ttcagaaaaa acattctttc tctcagcctg acaataaaat cagtggtctt ggtaacgaaa
     1381 gactgttcgt ttttgcctac ctttcgacaa aaaatcctct aaggccattt tcgacagtca
     1441 acttctagct ttttacacca accaaagcct ctcttgaacc attt
//
LOCUS       DACDCV010000219         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDCV010000219 DACDCV010000000
VERSION     DACDCV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.230"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IV041_002935"
     CDS             105..1406
                     /locus_tag="IV041_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8383640.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACDCV010000220         1441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDCV010000220 DACDCV010000000
VERSION     DACDCV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.231"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IV041_002936"
     CDS             complement(<1..625)
                     /locus_tag="IV041_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8383641.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IV041_002937"
     CDS             complement(784..1050)
                     /locus_tag="IV041_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383642.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IV041_002938"
     CDS             complement(1040..1396)
                     /locus_tag="IV041_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8383643.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          399 a          278 c          180 g          584 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 t
//
LOCUS       DACDCV010000221         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDCV010000221 DACDCV010000000
VERSION     DACDCV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.232"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV041_002939"
     CDS             complement(150..1337)
                     /locus_tag="IV041_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8383644.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDCV010000222         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDCV010000222 DACDCV010000000
VERSION     DACDCV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.233"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IV041_002940"
     rRNA            <1..>1355
                     /locus_tag="IV041_002940"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDCV010000223         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDCV010000223 DACDCV010000000
VERSION     DACDCV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.236"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IV041_002941"
     CDS             <1..848
                     /locus_tag="IV041_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8383645.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDCV010000224         1309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDCV010000224 DACDCV010000000
VERSION     DACDCV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.237"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IV041_002942"
     CDS             2..400
                     /locus_tag="IV041_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8383646.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IV041_002943"
     CDS             384..1079
                     /locus_tag="IV041_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8383647.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          494 a          197 c          263 g          355 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctc
//
LOCUS       DACDCV010000225         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDCV010000225 DACDCV010000000
VERSION     DACDCV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.238"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IV041_002944"
     CDS             52..>1302
                     /locus_tag="IV041_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8383648.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDCV010000226         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDCV010000226 DACDCV010000000
VERSION     DACDCV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.239"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..634)
                     /locus_tag="IV041_002945"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="IV041_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          406 a          208 c          216 g          462 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaacat gaggtggtat atgaatttga
     1141 aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta ttatatttat
     1201 ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt cacattaatc
     1261 gtgcgttaag tttagaatct atttttagct cc
//
LOCUS       DACDCV010000227         1290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDCV010000227 DACDCV010000000
VERSION     DACDCV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.240"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1169
                     /locus_tag="IV041_002946"
     CDS             <1..1169
                     /locus_tag="IV041_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8383649.1"
                     /translation="FYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQE
                     YDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIG
                     VEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLG
                     RKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDI
                     YAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNY
                     NFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLC
                     SQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          516 a          151 c          208 g          415 t
ORIGIN      
        1 ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt ctagagacag
       61 aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa gatggtacaa
      121 gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag
      181 atataccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag
      241 agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat
      301 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
      361 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
      421 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
      481 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
      541 tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag
      601 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
      661 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
      721 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac aatcaagatt
      781 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
      841 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatatagaaa
      901 tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta
      961 gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat
     1021 ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga
     1081 aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg
     1141 cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg
     1201 tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag
     1261 aaaaaagaga ggtaaatcaa atgaaaaaat
//
LOCUS       DACDCV010000228         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDCV010000228 DACDCV010000000
VERSION     DACDCV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.241"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..>1288
                     /locus_tag="IV041_002947"
     CDS             8..>1288
                     /locus_tag="IV041_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8383650.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          430 a          245 c          248 g          365 t
ORIGIN      
        1 atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat
       61 tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt
      121 ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg gaaacgggaa
      181 agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac aatacaatca
      241 catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact gtcgaaccca
      301 ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc atgtcagata
      361 taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga aaaattgtca
      421 ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact taccaaagca
      481 atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc acgcttccat
      541 agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa ttgatgtctt
      601 gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta atcctgaaga
      661 agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca aacgtattct
      721 gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga atcaagcctt
      781 taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga aaagtcaggc
      841 agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca tcaaccatta
      901 tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta cggaagcgat
      961 gatgattgat cgattgcttg gcttttctac gtccctgaag gaggcgtatc cctattttca
     1021 tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct tattggcaga
     1081 acttcccgaa acgttagatg acggctttcg ggaaaagctc caaaaccttc tgacctatga
     1141 agaaggcatc accaacgcga tgatctatcc ttacaccaat ggaaaaatag aagcgaagaa
     1201 tacccatata aagacaatga aacgagtatc ctacggattt aaatcatttg agaacatgag
     1261 aattagaatc tttttgatca atcaatta
//
LOCUS       DACDCV010000229         1254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDCV010000229 DACDCV010000000
VERSION     DACDCV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.242"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IV041_002948"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IV041_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IV041_002949"
     CDS             complement(546..740)
                     /locus_tag="IV041_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383651.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          445 a          212 c          171 g          426 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caag
//
LOCUS       DACDCV010000230         1248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDCV010000230 DACDCV010000000
VERSION     DACDCV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.243"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..1235
                     /locus_tag="IV041_002950"
     CDS             66..1235
                     /locus_tag="IV041_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8383652.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          454 a          188 c          203 g          403 t
ORIGIN      
        1 aaaaagaaag tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact
       61 ttctcatgcc caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag
      121 atgaattatt tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa
      181 cagcttttct tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta
      241 atgggaatta ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta
      301 gagatagaaa tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact
      361 ctttagaaac cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg
      421 aattgattga aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta
      481 aaatagtttc tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg
      541 tcatttttat ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag
      601 tttatattgt aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg
      661 ctccaactga atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt
      721 taaaagaggt tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata
      781 gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc
      841 ataaggttcg tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag
      901 caagttcaaa agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc
      961 agtatccacg agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc
     1021 ctccgtcaat cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat
     1081 taaagaaata taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc
     1141 tagtatctca attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta
     1201 aagaaataca tgatacatta gaatcaatgt tttaacttga tcaacaga
//
LOCUS       DACDCV010000231         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDCV010000231 DACDCV010000000
VERSION     DACDCV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.244"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV041_002951"
     CDS             200..742
                     /locus_tag="IV041_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383653.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV041_002952"
     CDS             832..>1246
                     /locus_tag="IV041_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8383654.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDCV010000232         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDCV010000232 DACDCV010000000
VERSION     DACDCV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.245"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV041_002953"
     CDS             <1..1244
                     /locus_tag="IV041_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8383655.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDCV010000233         1242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDCV010000233 DACDCV010000000
VERSION     DACDCV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.246"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1242
                     /locus_tag="IV041_002954"
     CDS             82..>1242
                     /locus_tag="IV041_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8383656.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIY"
BASE COUNT          413 a          243 c          239 g          347 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct at
//
LOCUS       DACDCV010000234         1229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDCV010000234 DACDCV010000000
VERSION     DACDCV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.247"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IV041_002955"
     CDS             330..1175
                     /locus_tag="IV041_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8383657.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          465 a          143 c          205 g          416 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcata
//
LOCUS       DACDCV010000235         1225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDCV010000235 DACDCV010000000
VERSION     DACDCV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.248"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..366
                     /locus_tag="IV041_002956"
                     /pseudo
     CDS             1..366
                     /locus_tag="IV041_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            424..>1225
                     /locus_tag="IV041_002957"
     CDS             424..>1225
                     /locus_tag="IV041_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383658.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVT"
BASE COUNT          428 a          161 c          173 g          463 t
ORIGIN      
        1 tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt
       61 accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg
      121 agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct
      181 cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg
      241 gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat gactatggta
      301 atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta
      361 attattaaat taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt
      421 cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa
      481 aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc agaattacta
      541 tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt taactttaaa
      601 aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat tctactttgt
      661 acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat tcaagctcct
      721 tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct atttatttct
      781 ccacagttgg aaaaattttc ttatacaagg agaagaaatt ttatactatt attaatatta
      841 gctatttcaa ttttaaatta ttcatcagta gttaacatgg tgaaagaaat gaattttcaa
      901 ggaacttatt ctaatgaaca aatggaaaat aaacttgaag aatttgagaa ttttgattat
      961 gtacctatag gctataaaaa agatcaattg cttttgaaaa accatcaagt tacagaagat
     1021 ggtcaagtta tttatagtag cttttctcca aataaagatg aaattacatt tacgtttaaa
     1081 tcttcaaaag gcaaaacaat agttttacct gtattccggt ataaaggatt aacggttact
     1141 gttaattcaa aaaatgctac agtactaaaa caaggaggtc cttttgttac agtaaaaagt
     1201 gaaagtggaa taaataaagt aacaa
//
LOCUS       DACDCV010000236         1223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDCV010000236 DACDCV010000000
VERSION     DACDCV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.249"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1223)
                     /gene="aph(2'')-I"
                     /locus_tag="IV041_002958"
     CDS             complement(<1..>1223)
                     /gene="aph(2'')-I"
                     /locus_tag="IV041_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP8383659.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEYYPIETIVYGI"
BASE COUNT          407 a          213 c           96 g          507 t
ORIGIN      
        1 ttaattccat aaacaatagt ttcaatagga taatattctt caactatatc ttgatattct
       61 tttgctttct caatatctat atttccatac attcttaata tatcttctcc aaaatttgtt
      121 cctatttctt cttcactatc ttcaagtaag tatataaaat cacaatattc atctataatt
      181 ccagaatctc caaaatcaat tattccagtt aatctattat tgccatctaa caatagatga
      241 ttacaactaa aatcattatg gcataaacac tttttaccct caaaaactgt tgttgcattt
      301 agtctttcca taaaactttc tatataatct ttttctatat cagttaaatc attataaata
      361 gtttcacgca acaatatata ctcttctaat acattttgtt tattatcaat agtacattca
      421 ctaatatctg tataatctaa accgtgcatt tgtcttaaaa aactggcaat atctcgtttt
      481 aacaaatttt gttcttcttc tgacatagta gaataaattt ctggtgttaa aaaagttcct
      541 ttaatttctt tataacctag tatagataat tcatcactaa tatacgaata ttcaatatta
      601 ggaattttta cattagtttc taaatttgta tttaaaaaat tatatattgc tttttctttt
      661 gcataacctt ttttcttatt agtactaaat tttgttttaa aaatgtattc attattaact
      721 aaatatgcca cactatcata accactaccg attatttcaa tactatctac tttgaaatta
      781 tcaaagtaat gctcaattaa atatttcatt gccttaacat ttgtggcatt atcatcatat
      841 ctatattcca ttaaataaca atcttctttt ttgccctcgt gtaattcatg ttctggcaaa
      901 tcttcaataa ttctaaaacc agatttttgg tatgccctta ttgctcttgg attattttta
      961 tgagggtcta aaataactgc attagcattt ctttcttttt tcaaaaattc aaaaatcaat
     1021 ttaatatatc ttgtaccaat tcctttactc caataatttg gctctcctat aaattgatcc
     1081 ataccataga ctatctcatc agtttttgga taatgataat cagtatataa ctcatcatac
     1141 attttatata tttgtccata tccaatagga acattgttat attcaataat tactctaaaa
     1201 acttcatctt cccaaggctc tgt
//
LOCUS       DACDCV010000237         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDCV010000237 DACDCV010000000
VERSION     DACDCV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.250"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            125..>1215
                     /locus_tag="IV041_002959"
     CDS             125..>1215
                     /locus_tag="IV041_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383660.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          400 a          124 c          214 g          477 t
ORIGIN      
        1 taaagaaaac ttgtatggaa aaatcagtac aataccatta tttaatgttc ttcacttaaa
       61 gcgcaaaaaa tatcagttta ttaaactcgt atatagaaca aatatatgta gacggggcga
      121 aacgatgagt gtaaaaaaaa tgctatttat ggtaagtaca ataattgtat taacatcccg
      181 attttgggga attaatacta gtttatctat gcgatatttt attatggcaa tatgggattt
      241 atattttatg ttaagctatt tttcttatcg tgagaaaaca tataaaaaat acgttatcaa
      301 gaataacttt tggttgacta taatgccgtt tgtgatattt tctatctata ctctaataat
      361 atggggaatt ggttcaaatg cagaatttga aaattttata aagttatgtt ccacgttgct
      421 ttatttaatt cttgcatatg gttacgcatg tacagcattt tacttattta aggcagaagg
      481 aattgatttt attttttggt caggtgttat ttcatatatg agtggttcag ttttttattt
      541 aattgtatct tatggtttaa atggtttaat ttcttatata aaaagtctta taacaggtga
      601 aacaaatact gcaaattttg ctatggaggt acatgattta acttttgcga tgggggtttt
      661 tttcctttat tacgtatttt ttgaaaataa aaatacaaaa aaacataaga taaaaattat
      721 cgtatcttta attttaattt tcatgggatt gaagagaatt gaactgttag ctttaagtgg
      781 tgcaattcta gtgtattatg ttttattaaa gtggggaaaa aagattcaaa tccgtgcatt
      841 agtgtgtact atttgtgcaa ctgttatttc gttagtgttt atttacatta tagatacagg
      901 aattttggca ttgcttatgt caaatttggg cattacagat gacggtgcga ggctttcatt
      961 ttatctatat tcatctaaat tttatgaact tggtttagga tatgtaggaa caggatggac
     1021 atggttttca aaatactttt ttaatcttta tttatcgcac tttaggttaa atggtggaag
     1081 aattgcagca agcctacaca gtgatatatt aactatttat atagaagttg gatgtatagt
     1141 ttttattata tggatgtttt ggttattttt tgtgaaaccg ataagactaa ctaaaaaatt
     1201 tggattgaaa acagg
//
LOCUS       DACDCV010000238         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDCV010000238 DACDCV010000000
VERSION     DACDCV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.251"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV041_002960"
     CDS             <1..1076
                     /locus_tag="IV041_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8383661.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDCV010000239         1186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDCV010000239 DACDCV010000000
VERSION     DACDCV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.252"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..953
                     /locus_tag="IV041_002961"
     CDS             39..953
                     /locus_tag="IV041_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383662.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEY
                     INDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKN
                     LSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILL
                     TIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1007..>1186)
                     /locus_tag="IV041_002962"
     CDS             complement(1007..>1186)
                     /locus_tag="IV041_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8383663.1"
                     /translation="SNGLLRKDGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTP
                     LEVFLSYVNGKFCLA"
BASE COUNT          410 a          178 c          205 g          393 t
ORIGIN      
        1 aattgtatac gctacctata attataaaaa cagaaaaaat gttaaaagat ttaaaacagt
       61 attatcctct gtctgcttat gaaaaaaaga aattattcca ctttttagca atcaacctgt
      121 tggctgaaca agctgattct ttagatattg ctagcaatca gtcgctattt ttttcggata
      181 taggatttta ccacttaaaa ggagtattag gagaaaaatc cgttattttt atcaataaga
      241 cactctcttg gctttatctt catgataaat tggatcaaaa atacttgaga aaaaatgaag
      301 atgaaacaat cgaatggttg aatgaacgat tcattgaaag ttttgaaaat cagtttaatc
      361 tgttgcgaga agagacgagc caattgatcc gaagagagtt aaccaaaata cattttaaac
      421 ttttgcatta cactattaat aaagctgatg aatttccact tgatgtttct ttttttagag
      481 aaaactatcc agcattttat tgctatttga tagagtatat aaatgatcta acgtcaaaac
      541 gcaaagagtt gcttcaagat aaattttttc tttttttcag ttatttatta ttattgatca
      601 atcacatacc cattcaactg atttctgaac cagtaaagat catcatcgat ttttcttatg
      661 gtgctgccta taatcaattt atcaagaaaa atttaagctt gtacattaat ctgaacacgg
      721 aagtgataga ggcaggaaag cctgagttcc cggatgttat aatcacgaat aggaataatc
      781 tatatgaaga aagtgcatct caagtagtgg tttggctcga tccgccaaga gcagtagact
      841 gggggaatct tacaaagatt ttgttgacta tccaagaaga gaaatacaaa gacagcaaaa
      901 ttgacaagca aatagacgat tttcctgaag aaatcatcga caggatcgaa tagtgaactt
      961 cttttagaaa gtccgactgc tttttctaaa aatattattt gtcaaattaa gcgagacaaa
     1021 actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt agtgattttc
     1081 tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg acttggttga
     1141 attccatttc ttttggtagt ccatcttttc ggaggagtcc gtttga
//
LOCUS       DACDCV010000240         1180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDCV010000240 DACDCV010000000
VERSION     DACDCV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.253"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..845)
                     /locus_tag="IV041_002963"
     CDS             complement(<1..845)
                     /locus_tag="IV041_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383664.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLD"
BASE COUNT          389 a          212 c          119 g          460 t
ORIGIN      
        1 aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt aataatgttg
       61 tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc aatttcctcc
      121 caataatatg aatctctttc aattcccatg atggtagctt tggctaattt caaatcaaat
      181 aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc tttaatccat
      241 tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt atatttcaaa
      301 tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct tttaagtact
      361 tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt attcactaat
      421 aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg aaaatcacct
      481 ttataaactc cattcttttc cactgtttta ttaatatttt ttaaaaacca gaatgagttt
      541 gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg taataataag
      601 acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc aatcataggg
      661 agccattttt tgattttcac taattgattg ctcataaatc tttcactgga cttttgttca
      721 atcttaaatt gcaaaacttc taactcataa ttcccatctt ctgatttatt tttgggaaca
      781 actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc ataatttgta
      841 gccattaata catctcctta ttcataataa atttcatatg acgctcctct caaaatttac
      901 cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct taaaaaaaaa
      961 ctggtctctc taccggtaat atcgattgag tccgccttta tcgactttat ccttttttta
     1021 tattaaattt tttcaccggt agacagggag agaaagaata aactaaaaag cccttaattc
     1081 ttaatgtttt cacctcctta ccgttcctac cgctaaaatt taatataact aaattctata
     1141 acattagaag aaattttaaa tacctttgat aaaaaatatt
//
LOCUS       DACDCV010000241         1168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDCV010000241 DACDCV010000000
VERSION     DACDCV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.254"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1010
                     /locus_tag="IV041_002964"
     CDS             63..1010
                     /locus_tag="IV041_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8383665.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          382 a          175 c          252 g          359 t
ORIGIN      
        1 atttttagaa gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa
       61 ggatgagctg ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc
      121 atttaggaga agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa
      181 atcttgatat gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga
      241 tggaagagat catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta
      301 gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat
      361 taatgatttt agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt
      421 cttcagcggc tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc
      481 gcccattgac gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt
      541 gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc
      601 aaaatccgaa ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac
      661 aaatcgtagg agaagcaact tcatttacat tatttggtga tggaagccag tctagagact
      721 ttgtttatat agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac
      781 tggggaaaca attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg
      841 caatcaatga aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg
      901 atatccatga ctctctagca gatattacta agatccagtc aattggctat caaccgaaat
      961 atggtatttt aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa
     1021 gataagatta gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa
     1081 tcaaatctgg gatagaaggg ctctttgtca aatgatgttg gtaaagagaa atcttagaat
     1141 aatttggaca gaacagaaag aagaaaac
//
LOCUS       DACDCV010000242         1166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDCV010000242 DACDCV010000000
VERSION     DACDCV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.255"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..592
                     /locus_tag="IV041_002965"
     CDS             <1..592
                     /locus_tag="IV041_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383666.1"
                     /translation="SDLNKGILYGLLFNILLVLDGSKKWETCLNEFDKLNDLVIAEKS
                     KFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWINSNAIHSGEFFHG
                     PFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADFDLESIDSVAKEY
                     IAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            574..1035
                     /locus_tag="IV041_002966"
     CDS             574..1035
                     /locus_tag="IV041_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8383667.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1085..>1166
                     /locus_tag="IV041_002967"
     CDS             1085..>1166
                     /locus_tag="IV041_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8383668.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDIN"
BASE COUNT          416 a          145 c          215 g          390 t
ORIGIN      
        1 tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac ttgttttaga
       61 tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg atcttgttat
      121 tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc aaaaacgaga
      181 taaaattatt tacacaattg gtagcggtat caattacgga gaaatttact ccacagcagc
      241 ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg gggaattttt
      301 ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga aaagtattgg
      361 agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca gtaataaatt
      421 aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag cgaaagaata
      481 tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga ttgcttttga
      541 aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt aattaatagg
      601 aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca ttctaccctt
      661 tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac aggtattagt
      721 gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat gaaagaaata
      781 attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg aggaacacct
      841 aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc tgggattaat
      901 ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat agaagaaata
      961 gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa ttcgctttta
     1021 aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg aaggaggaaa
     1081 tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag taatattttc
     1141 ttggactaaa cagtatgata ttaacc
//
LOCUS       DACDCV010000243         1141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDCV010000243 DACDCV010000000
VERSION     DACDCV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.256"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IV041_002968"
     CDS             <1..354
                     /locus_tag="IV041_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8383669.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IV041_002969"
     CDS             355..903
                     /locus_tag="IV041_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383670.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          446 a          147 c          242 g          306 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcgtga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 a
//
LOCUS       DACDCV010000244         1140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDCV010000244 DACDCV010000000
VERSION     DACDCV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.257"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..709
                     /gene="lexA"
                     /locus_tag="IV041_002970"
     CDS             86..709
                     /gene="lexA"
                     /locus_tag="IV041_002970"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8383671.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            889..1107
                     /locus_tag="IV041_002971"
     CDS             889..1107
                     /locus_tag="IV041_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383672.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          355 a          216 c          220 g          349 t
ORIGIN      
        1 aaactaaata aaatacgaac attcgtttga tttagcgctt tttatatggt actatttatt
       61 tatcaagtga aagaaggtgt accttgtggc ccgacaaact gaatcaagac aaatcgaagt
      121 cttacgatat atccatgaac aagtaaccca aaaaggatac ccgccgactg tccgtgaaat
      181 cggggaagct gttcagcttt cttctacttc tactgttcac ggtcatcttg cacggcttga
      241 aaaaaaaggg tttatccaaa gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca
      301 aggtttagaa aagattggga ttcgtccaac aactattcca gtacttggcg tcgtaacagc
      361 tggagaaccc attttagcag tagaggatgc ttcagacttc tttccaatcc ctcctaatct
      421 ttcttccgaa gaaggcagtc tctttatgct gacaattcgt ggagatagta tgatcaatgc
      481 tgggatattg gatggtgaca acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga
      541 tattgtcatt gcaatgactg ccgaggacga agcgacttgc aaacgattct ataaagaaaa
      601 aaactttttc cgtctgcagc ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt
      661 atctatatta ggacgagtag taggacttta tcgtagtcat atctattaat tgctactaat
      721 taaaagaaaa ctttttaaaa agagtttgag acgttggtct caaactcttt tttcgcccgg
      781 gttcttcttc tttcaaattt ttatcacttc cctctttaca aaccgaacaa atattcgtat
      841 actaaatata cgaacaattg ttcgcataaa ttaaagaggt gagaattcat gaaatcaatg
      901 agacgtcttg gcttagtatt aactgttttg tttttaggta ttattattgg taatttcggc
      961 atacgtccag ctttacttgt gtttgttccg ttatttactt tgtggttcat gttgtgggat
     1021 gaaaagaagt accgtcaatc tgagcggcgc agaattcatg aagatcattt ctatcatgag
     1081 ccctactaca gatccaggca atcataataa ggattgactg ctaattattc aaaagacaag
//
LOCUS       DACDCV010000245         1081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDCV010000245 DACDCV010000000
VERSION     DACDCV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.258"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IV041_002972"
     CDS             <1..370
                     /locus_tag="IV041_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8383673.1"
                     /translation="KARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQDIIS
                     TRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRSFVPR
                     EIRCVARNLIYLQNNNILNS"
     gene            384..740
                     /locus_tag="IV041_002973"
     CDS             384..740
                     /locus_tag="IV041_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8383674.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          378 a          162 c          206 g          335 t
ORIGIN      
        1 aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc
       61 cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca
      121 agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt
      181 aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat
      241 tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt
      301 ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt
      361 aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa
      421 caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact
      481 tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc
      541 aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt
      601 cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac
      661 gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac
      721 agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca
      781 gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt
      841 ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata
      901 gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa
      961 gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga
     1021 aatgacatga taagataact gagttgttaa aaaagaacca cttgcgaagc ttgattctat
     1081 c
//
LOCUS       DACDCV010000246         1080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDCV010000246 DACDCV010000000
VERSION     DACDCV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.259"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV041_002974"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV041_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8383675.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          321 a          221 c          183 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
//
LOCUS       DACDCV010000247         1056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDCV010000247 DACDCV010000000
VERSION     DACDCV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.260"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..686
                     /locus_tag="IV041_002975"
     CDS             <1..686
                     /locus_tag="IV041_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8383676.1"
                     /translation="FRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVS
                     IDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYG
                     ETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVL
                     NIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSI
                     SYLYLKKEN"
     gene            688..>1056
                     /locus_tag="IV041_002976"
     CDS             688..>1056
                     /locus_tag="IV041_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383677.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDG"
BASE COUNT          364 a          125 c          179 g          388 t
ORIGIN      
        1 aatttagaaa gagcattaga gaagtactac ctgtaaaaat caaatggatg gtaagcatat
       61 tattagttct attggtatat ttttcttttg gtactaatta tgctaaggat tttttagcaa
      121 caattcctgt atcgattgat agaaaagtag aagttataga gtcaaatgaa tcagataaaa
      181 ctccacttcc agaaaaagaa gtaatattac agcattctag tgataactcc gaaatttcag
      241 ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat aagaccaact ttgttatttg
      301 gttatggaga gaccgatttc tatgggaata gcgatgcaaa atttttgaaa aaagtagatc
      361 ttgatactac agatgtgaat gaactaaaaa gagcacatgg aaacatgcat aatggatttc
      421 ttcaagtact tgtatcaagt ggtataattg cttttatatg tatctatgct ttctatgtgt
      481 taaatatctt tagtttactc aaaacatttt ttagaagtaa agtgaattat gcgtttcttg
      541 gtattgtttt aatttttata ctatctattt ttatagatga aatggtggag gctcatgtac
      601 ttttcaataa gcgcgatgtc atttcaattg ttttctggta ttatttggga agcatttctt
      661 acttgtattt aaaaaaggag aattaatatg aagacagctt tcattgcttg gactccttta
      721 catttgatta atatattaaa tgtttatcaa aatttttacc gagaatcaaa aggagattta
      781 tttatttatt ctgaatttga aaacgctaga agatatagtg agcgtataaa agaattaggg
      841 ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa
      901 attaacttgt taactaacca taatcaattt ttctcggatg atttatccgt ttatgatgaa
      961 atttttatac agggggaaaa ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa
     1021 aatccagatg taaaactaaa ttatatagaa gatggt
//
LOCUS       DACDCV010000248          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDCV010000248 DACDCV010000000
VERSION     DACDCV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.261"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IV041_002977"
     CDS             complement(26..913)
                     /locus_tag="IV041_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8383678.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDCV010000249          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDCV010000249 DACDCV010000000
VERSION     DACDCV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.262"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV041_002978"
     rRNA            complement(<1..>994)
                     /locus_tag="IV041_002978"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDCV010000250          989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDCV010000250 DACDCV010000000
VERSION     DACDCV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.263"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            350..973
                     /locus_tag="IV041_002979"
     CDS             350..973
                     /locus_tag="IV041_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383679.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          366 a          137 c          180 g          306 t
ORIGIN      
        1 cattgcaaga ttccttacta aattagtttc tgaatggaaa agtattattt ctcaaaatat
       61 ggataaatta agatattaag gatgttagtt ttaaaataaa ctaatgtcct tatttttgtg
      121 ttcacatttc tgttacctaa gaaatactta aaggaggtga tgtagccagc aacgactaca
      181 acttgaaccc gctatctaca gtttatttgt tgtttctttt gggtatactc taatggtaca
      241 aatccatatt tgcttaaagc gttgattttt cagcggtttt tctttattgc aaagtgtacc
      301 ctaagacctg tatactaata gtaaatagat tggataggaa tgatggcaaa tgtatttagc
      361 ttatttaaga gtttcttccg acgatcaaag tttagctcgg caacataagc taattgaaaa
      421 ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa attttcgagg aaaaagtctc
      481 tggaaaaaat attgaaaaca gaccagagct gcaagagctt atttctttca tacgtgaacg
      541 agatacgatt attattccta gtcttgatcg attagcaaga aatagtaaag atacaaaaga
      601 cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa atattagacc taccgtcgtt
      661 taatggggta actgaccctg cattaagaga cttgctaaca aatttagtca tagaagtgtt
      721 tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag cgtcaaaaac aaggcattga
      781 aattgcaaag aaaaatggtc gttatactgg taaaaagatt caataccatg ccaatgcaac
      841 aggaaaaaac aaattgattt acgatgaaat tgttgatggt ctgaagaaaa aagaaactat
      901 ttctaaaata gcaaagaacg caggagttac aagaaaaacc gtttatcgag taaaaagaga
      961 tttaggaatg taatatgttc cagttagaa
//
LOCUS       DACDCV010000251          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDCV010000251 DACDCV010000000
VERSION     DACDCV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.264"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV041_002980"
     CDS             <1..704
                     /locus_tag="IV041_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8383680.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DACDCV010000252          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDCV010000252 DACDCV010000000
VERSION     DACDCV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.265"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IV041_002981"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IV041_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IV041_002982"
     CDS             complement(570..>959)
                     /locus_tag="IV041_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8383681.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACDCV010000253          948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDCV010000253 DACDCV010000000
VERSION     DACDCV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.266"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..731
                     /locus_tag="IV041_002983"
     CDS             <1..731
                     /locus_tag="IV041_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8383682.1"
                     /translation="TIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMV
                     FNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELA
                     VSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFS
                     VSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRV
                     FKKYAGVSPKQYQLGLVDNFNNIS"
     gene            748..>948
                     /locus_tag="IV041_002984"
     CDS             748..>948
                     /locus_tag="IV041_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383683.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESI"
BASE COUNT          353 a          107 c          169 g          319 t
ORIGIN      
        1 aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat ccagaaatat
       61 atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat attcgaaatt
      121 tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt ggagatacga
      181 ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa acgtctataa
      241 cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata caagaaaacg
      301 aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt gtttattata
      361 ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct tctgtcgttc
      421 aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct aattattttt
      481 ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt acgatttata
      541 attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca aaccatacta
      601 tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg agagtattca
      661 aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac aatttcaaca
      721 acattagctg agaggtttta ttttattatg agactatatc atttacgtag tttggaggag
      781 ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt gagttgtttc
      841 gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata tgacaaagta
      901 gtaattaata aagtaaaaag tttatgtgaa gaagctagag aatctatc
//
LOCUS       DACDCV010000254          931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDCV010000254 DACDCV010000000
VERSION     DACDCV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.267"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..790)
                     /locus_tag="IV041_002985"
     CDS             complement(56..790)
                     /locus_tag="IV041_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8383684.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          321 a          187 c          178 g          245 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa a
//
LOCUS       DACDCV010000255          892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDCV010000255 DACDCV010000000
VERSION     DACDCV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.268"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..>892
                     /locus_tag="IV041_002986"
     CDS             6..>892
                     /locus_tag="IV041_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8383685.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITA"
BASE COUNT          303 a          122 c          163 g          304 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gc
//
LOCUS       DACDCV010000256          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDCV010000256 DACDCV010000000
VERSION     DACDCV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.269"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV041_002987"
     CDS             <1..888
                     /locus_tag="IV041_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8383686.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDCV010000257          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDCV010000257 DACDCV010000000
VERSION     DACDCV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.270"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            734..>861
                     /locus_tag="IV041_002988"
     CDS             734..>861
                     /locus_tag="IV041_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8383687.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQK"
BASE COUNT          312 a          138 c          109 g          302 t
ORIGIN      
        1 taggtaactt taaacaaaca tgaagagact ttaataagac ttctttatct gaaaacactt
       61 ttgtttctct ctttcctttc tacatcatta aatagtattt tatacgttat ctaaaatact
      121 attcctctgt caaaataagc taacttactt cattataaaa tatccactta tcgctttcat
      181 gtatattcca ccgtaaaaag tacattttat caccgagaac tcatcatttt tcatcatgat
      241 gaatactatt tacatatatc tagttcaaag acaataatta ttatgatccg ttattatcta
      301 agcactatac agataatagg aatttttcgt ttctaaatat tccaatttgg gaatccaata
      361 aaaattttcg ttcaaatacg tgtctattcg aactcttttc tagttgttaa ttatgaaggg
      421 ggaagaaata aaaaatacaa tctatacacg aataagaata ttatccaaaa ggaggtttta
      481 gaaaagattt attatacgta aaaagctcaa gacattttct tagccataag gacatgtttg
      541 atatttaaga agatatatga attttctttt ctaagtataa agataactat ccattatcaa
      601 ctaaaaaaac atttttgaaa ataaacatca gcagtcctat caatgcatgt gagcttatag
      661 aagttattcg cagcgcagca taattgcatt agcttctttt cgtgtttttt gcctcttttc
      721 cctttaaatc ttaatggagg aggtgtgcaa tatgtcagat aacgacgaac gcgtaaatgc
      781 ccagttgatt cgattctttg aaaaggtcat tttgaacacc gctaatactt attataaaaa
      841 acaagaagaa attcaaaaac a
//
LOCUS       DACDCV010000258          834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDCV010000258 DACDCV010000000
VERSION     DACDCV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.271"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            118..702
                     /locus_tag="IV041_002989"
     CDS             118..702
                     /locus_tag="IV041_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8383688.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          270 a          115 c          173 g          276 t
ORIGIN      
        1 tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc
       61 agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg
      121 aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc
      181 ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat
      241 cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg
      301 cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat
      361 gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct
      421 acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc
      481 ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt
      541 cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca
      601 cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg
      661 gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct
      721 gaaaattttt gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc
      781 tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat atta
//
LOCUS       DACDCV010000259          831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDCV010000259 DACDCV010000000
VERSION     DACDCV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.272"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV041_002990"
     CDS             <1..395
                     /locus_tag="IV041_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8383689.1"
                     /translation="HELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKI
                     IEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            396..>831
                     /locus_tag="IV041_002991"
     CDS             396..>831
                     /locus_tag="IV041_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8383690.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          322 a           92 c          144 g          273 t
ORIGIN      
        1 tacatgaatt ggattatatt ataaacctat ttggtttccc taaatcagta aaaaaacagt
       61 ttggtaaata ttcgaatctt gaaattaaca gtaatgattt agcaacatat atattgagat
      121 acgatgataa aattatagaa attcatttag attattttgg aagagttcct acaagaaaat
      181 ttcaattgtt tactttagat gaatgtatca atgttgactt attaaataat aaaataagtt
      241 ttgaagaaaa cattgttaaa gaaatgcctg aatctgctaa tgatatgtat gagaaggagt
      301 tgaaatattt ctttgaaaac gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg
      361 caaacgaagt attgaaaata gctgaggaga aataaatgga aattttattt actatttgtg
      421 gtagagctgg gtctaagggg tttaaaaata aaaatttgaa atatttggca ggaaagcccc
      481 tagtccattt cgctttagca atgatagata tctatcaaca aaaacatcct gaacataatt
      541 gcacgacttg tttgaatacg gacagtaatg agttagtaga attgacgact aagttaaatc
      601 cgtctgtgaa aattgtagaa cgtaaacctg aattagctac agatactgta gggaaaattg
      661 aagtgatacg tgattcattc ttacaactag aaagagaaga taatatattt gatttaatta
      721 tagatttaga tttgacttca cctttacgtg aagtttctaa tcttgaagaa ttaatagatg
      781 aacatctaaa aaatttagat aaagacgtct acttttcagt tgtttctgca a
//
LOCUS       DACDCV010000260          831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDCV010000260 DACDCV010000000
VERSION     DACDCV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.273"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>831
                     /locus_tag="IV041_002992"
     CDS             2..>831
                     /locus_tag="IV041_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8383691.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          285 a          120 c          152 g          274 t
ORIGIN      
        1 tatgaacgat tttactacag aaattatgga aatactaatc aataaaggtg atttggatga
       61 tttgtttcgt cgtcacctag aattggctat taacgcttta ttacaagctg aattaacggt
      121 atttcttgac tatgaaaagt atgatagagc tggatttaat tcaggtaatt cccgtaatgg
      181 gaattattcg cgctctttta aaacagagta cggtgaattg aatttagtaa tacctagaga
      241 taggaatgga gacttttctc aacaaacttt acctgcttat aaaagaacca atgactcttt
      301 agaaaccact attattcagc tagttcaaaa aggaattact atgtccgaaa tctctgatct
      361 aatcgaaaaa atgtatggtc attattacaa accacaaact atttcaaata tgagtaaaat
      421 tgtgtctgaa gatgtttcgg catttaaaga aagaacttta gaagctaaat actcagtcat
      481 ttttatggat gctactcata ttcccttaaa aagacaaacc gtatcaaaag aagctgtcta
      541 tatagtaatt ggtatcaggt tggatggaac taaagaggtt ctaggattta ctatagctcc
      601 aactgaatct gcttatgttt ggaaagagat tcttcaagat ctaaaagacc gtggtttaga
      661 agaggttttg ttagtcgtaa cagatggttt aagtggtatt gacgatagta ttcatagtgt
      721 ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata tcgctcataa
      781 ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt a
//
LOCUS       DACDCV010000261          803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDCV010000261 DACDCV010000000
VERSION     DACDCV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.274"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..793)
                     /locus_tag="IV041_002993"
     CDS             complement(<1..793)
                     /locus_tag="IV041_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383692.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENE"
BASE COUNT          268 a           96 c          143 g          296 t
ORIGIN      
        1 attcattttc tagcacgaga cgttttgctt tttccatttt tagttcttta aaatagctaa
       61 taacactttt tccattcact tttctaaaat gtttttttaa gctggctgga ctgataaaaa
      121 aaatttgggc taattgttca agcaaaaaat tttctgaaag atgttcttct aaatatcgta
      181 tgattggatc aactaattca tctgaataaa gaatatgctt ttttttagga atgactttct
      241 catttgaagg tattgtcaat aaagcaagtt cctgttgata atgccttggt aaggaagaca
      301 attgaatttt ttgcagacaa taagtaacta ataaggaaag caaatgaatg ctcatctgtt
      361 ggcatagtat gaaaaaagaa ttgatttcct gatcatagga aatagctggc ttactatgtt
      421 ttagataatc aattttgaac tgttcatttt tcagaaaagt aatgatagat gttatttctt
      481 gatctataga aaaagattgt cccgtaatat gtgctaataa ttgctcatca aaataaaact
      541 ggataatgat aaaagtttgt gtttcctttt gagtagaaaa gatttggact tcttgctgtt
      601 tatcaatgaa taaaagggaa ggattatcta gttctataga tttatgtgca aattcagcat
      661 ataaattagc gctttcattt attagtagtc gaaaataggg agcagcagat aaatgaagat
      721 gggtatctat ggaataggtt agtgtttttt tgacaagatt ggaggattgg ttgtttgtta
      781 acaaaagaac cactttcctt tct
//
LOCUS       DACDCV010000262          802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDCV010000262 DACDCV010000000
VERSION     DACDCV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.275"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>802
                     /locus_tag="IV041_002994"
     CDS             <1..>802
                     /locus_tag="IV041_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383693.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNG"
BASE COUNT          296 a          154 c          160 g          192 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aa
//
LOCUS       DACDCV010000263          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDCV010000263 DACDCV010000000
VERSION     DACDCV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.276"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..>772)
                     /locus_tag="IV041_002995"
     CDS             complement(109..>772)
                     /locus_tag="IV041_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8383694.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          213 a          178 c          149 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gc
//
LOCUS       DACDCV010000264          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDCV010000264 DACDCV010000000
VERSION     DACDCV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.277"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            178..381
                     /locus_tag="IV041_002996"
     CDS             178..381
                     /locus_tag="IV041_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8383695.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="IV041_002997"
     CDS             381..731
                     /locus_tag="IV041_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8383696.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DACDCV010000265          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDCV010000265 DACDCV010000000
VERSION     DACDCV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.278"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..690
                     /locus_tag="IV041_002998"
     CDS             73..690
                     /locus_tag="IV041_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383697.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          269 a          100 c          142 g          230 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaata c
//
LOCUS       DACDCV010000266          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDCV010000266 DACDCV010000000
VERSION     DACDCV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.279"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IV041_002999"
     rRNA            2..>733
                     /locus_tag="IV041_002999"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDCV010000267          723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDCV010000267 DACDCV010000000
VERSION     DACDCV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.280"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IV041_003000"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IV041_003000"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="IV041_003001"
     tRNA            207..279
                     /locus_tag="IV041_003001"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="IV041_003002"
     tRNA            283..355
                     /locus_tag="IV041_003002"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="IV041_003003"
     tRNA            363..444
                     /locus_tag="IV041_003003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="IV041_003004"
     tRNA            462..534
                     /locus_tag="IV041_003004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="IV041_003005"
     tRNA            546..617
                     /locus_tag="IV041_003005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="IV041_003006"
     tRNA            634..719
                     /locus_tag="IV041_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
BASE COUNT          175 a          168 c          199 g          181 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttc
//
LOCUS       DACDCV010000268          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDCV010000268 DACDCV010000000
VERSION     DACDCV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.281"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IV041_003007"
     CDS             complement(<1..446)
                     /locus_tag="IV041_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8383698.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACDCV010000269          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDCV010000269 DACDCV010000000
VERSION     DACDCV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.282"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IV041_003008"
     CDS             99..293
                     /locus_tag="IV041_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383699.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          290 a           93 c          120 g          209 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aa
//
LOCUS       DACDCV010000270          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDCV010000270 DACDCV010000000
VERSION     DACDCV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.283"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..391
                     /locus_tag="IV041_003009"
                     /pseudo
     CDS             1..391
                     /locus_tag="IV041_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
BASE COUNT          258 a           80 c          133 g          238 t
ORIGIN      
        1 taattattta gaaagtgacg aatttatagc attaagaaac tatgttgtta ataaaatatc
       61 accaattatt gattcgacag gtctatgttt attagatgaa ttcttggcgg atatcagtct
      121 atttataatg tttattgatg taattgaaga aacatcaaga aattttttgc tgtctaagtt
      181 cagaaaattt cgtatgaaaa atgataaaca aaaattattg gcaaatgaga tttggagtag
      241 tttagaagtc gaagaaagta aacaagaact tttgttgaaa tggatacttt ttcttgtaaa
      301 gaagttctat gtgaaaatac cagaaaccca ttgtctgata gttatgcacg gtaaaataac
      361 agcttctgcg atcgcaaggg aaacaaataa gttgctgaat acatatgttt atgaggcttt
      421 cgatatgcca attgaaggtg agactagtga cttaattaac ctaatcaaca atttttgtaa
      481 aagtatagat acgacgaatg gactcattct gctagttgat atgggatcat tggaacaaat
      541 gtatgaaaaa attgaatcca atgtaatagg tgatttggta attttaaaca atgtttctac
      601 agctttagcg attgaatgtg ggattcaagt ttgccaaaaa aagccaatta gtcattttta
      661 ccagatggat tttgattcgt ttcaagttaa agttcagtat tataaagga
//
LOCUS       DACDCV010000271          703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDCV010000271 DACDCV010000000
VERSION     DACDCV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.284"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..703)
                     /locus_tag="IV041_003010"
                     /pseudo
     CDS             complement(83..703)
                     /locus_tag="IV041_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          173 a          146 c          131 g          253 t
ORIGIN      
        1 tcccattcaa cgcaaaaaaa taaatagtga cggtttttcc ttcacgataa agctttgctg
       61 atccggtatt cccgtttgta actgttaagg ttacgacttc atatttgttg tcgtctgatg
      121 tcaatacaga ttttcctttt gattgaatac catcttcgaa atttttcaat ccttcgatgg
      181 attgcggttc agtcaaacta accgtatcat tcaagccttt ttcagtatat tcaggcgtga
      241 tgtcccagct gtagtcgttc ggattgttgc tatctttcaa tccttcacca aagtatttaa
      301 actgactaat attcggggtt cgggtgtcgc ctttttcaat tttgagccag tcaatttggc
      361 atgcgccggc cgtttcttta ggcaactgaa aaatgcgaag gtctttaggc aaacccggtt
      421 caagtttcgt tggtgtgaat gttagagacc atacgtctgt caatccctca actggtttta
      481 gttctccaaa attaacgttc caataattat atgctacaaa ggtttgactt gcgggttttg
      541 ttcctttaag cgtgatagta tacgtttgac ctattataag ctcttcttcc gtgttacctt
      601 tatatatttc gtacgcgcta gatttaattg ggaacgtcgt acctttattt gcaatattct
      661 cacccaacga tgttttacct acccagtaag ggtcatctag taa
//
LOCUS       DACDCV010000272          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDCV010000272 DACDCV010000000
VERSION     DACDCV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.285"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IV041_003011"
     CDS             132..344
                     /locus_tag="IV041_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383700.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>689
                     /locus_tag="IV041_003012"
     CDS             506..>689
                     /locus_tag="IV041_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383701.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFV"
BASE COUNT          245 a          103 c          121 g          220 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttc
//
LOCUS       DACDCV010000273          657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDCV010000273 DACDCV010000000
VERSION     DACDCV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.286"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..628
                     /locus_tag="IV041_003013"
     CDS             <1..628
                     /locus_tag="IV041_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8383702.1"
                     /translation="KQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISILNSQL
                     SSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQNEINA
                     KIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNIQNQL
                     QATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          317 a           83 c           94 g          163 t
ORIGIN      
        1 aaagcaatat gctgataata ttttattatg gggaggggaa gataatattg aaaagatcaa
       61 caacaattta actatattag acacagctct agaaaataaa gagcaaaaaa ttagtatttt
      121 aaattcacaa ttgtcttcaa acaaccaaac tcttgaacaa ctaagaacta atattgaagt
      181 ttccaaacaa aaagtggcat ctttagaaaa cgacaagaat caattagtat cagagaaaac
      241 taatttagaa aatcaattgt cagcaaaaga ctctgaattg caaggaaaac aaaatgaaat
      301 taatgcgaaa atagacgaaa ttaaccaaaa agtagctgaa ataaatcgat taaatagcga
      361 ttggaataat cgtttacaaa atgaaaagaa caaatttaat gaagaacaat ctactattaa
      421 tagtttaaac cagcaaatta atcaaatgtt gagtgaaaaa aataacattc aaaatcaact
      481 gcaagctact cagcaaaagc tagaagaaga aaagtcaaaa aatagtagtt tgaataatta
      541 cattgaaaag cttcaaaaag ctaaaaatga tgtagaggat accgcaaata aagcacaaca
      601 aattgtaaat gaacatacaa tccaatagat atagttttaa tttaacaaat ggtatgt
//
LOCUS       DACDCV010000274          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDCV010000274 DACDCV010000000
VERSION     DACDCV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.287"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..623
                     /gene="spxA"
                     /locus_tag="IV041_003014"
     CDS             225..623
                     /gene="spxA"
                     /locus_tag="IV041_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8383703.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          234 a           96 c          122 g          198 t
ORIGIN      
        1 aaaatttgaa ttagatttga aaactctaat ttttaaatga taaaaaaagc gagaatgctg
       61 gttttactgt atattaacct tagataactt tcattttttt aaaaaaatta tgaaaaaaag
      121 gtagctattt aaaagttgat tatgtataat agaaacagat gggagattat tatgatcttc
      181 tgttacaaaa aagtgattct tatcacgaaa aggagtggtg cacgttgtta acattatata
      241 cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc
      301 catttgttga acgtaatatt ttttcagaac cattgaacag ttctgaatta aaagcgattt
      361 tacaaatgac agaagacgga acagaagaaa tcatctcgac acgttccaaa gtattccaaa
      421 aactgaacat ggatttagat gatctgccgt tacaagaatt attggaactt gtgcaaaaca
      481 atcctggatt gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa gttggtttta
      541 atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag
      601 cacaattgat ggctggatta tagaaggata atacgaaact tggaaaagac
//
LOCUS       DACDCV010000275          643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDCV010000275 DACDCV010000000
VERSION     DACDCV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.288"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(75..>643)
                     /locus_tag="IV041_003015"
     CDS             complement(75..>643)
                     /locus_tag="IV041_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383704.1"
                     /translation="TIKFFKHYNIIDIGDYSMEEKKIYKIEQNLELELLNESHYEEIY
                     NFITDHPESMYFHEDIYHQGNSKELEVVLRYNLFSFTSEFYFLRHKERIVGMVSIIVN
                     PFQNLSYSIVNYLICEPAYTTTILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPY
                     LKDSHFFKEAVLSKEYREHDVLRYAYIY"
BASE COUNT          225 a           93 c           67 g          258 t
ORIGIN      
        1 aataaaatca cttcttttcc ctcctatatc ttatgatatg atccgaatta ttaaaatact
       61 atcagaaatt ttatttaata aatgtaagcg tatctcaaaa catcatgctc tctatattct
      121 ttagatagta ccgcttcttt aaaaaaatgt gaatctttta aataggggat tagtgattca
      181 tcttctgttg aatttagcaa atgaatcact agcttcttta cttctaaaat tgaaacttcg
      241 ttaatatttt ctaatactgt tgtcaaaata gtcgtagtat atgcaggctc acatatgagg
      301 taatttacta tactgtagga taagttttga aatggattaa caattataga aaccattcct
      361 acaattctct ctttatgtct taaaaagtaa aattcactcg taaacgaaaa taaattatat
      421 ctaaggacaa cttctaattc tttactattt ccttgatgat aaatatcttc atgaaaatac
      481 atactttcag gatgatctgt gataaaatta taaatttctt cataatgtga ttcatttagt
      541 aattctaatt ctaaattttg ttcaatttta tatattttct tttcttccat actataatct
      601 cctatatcaa taatattata gtgtttgaaa aatttaatag ttc
//
LOCUS       DACDCV010000276          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDCV010000276 DACDCV010000000
VERSION     DACDCV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.289"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..625
                     /locus_tag="IV041_003016"
                     /pseudo
     CDS             1..625
                     /locus_tag="IV041_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DACDCV010000277          589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDCV010000277 DACDCV010000000
VERSION     DACDCV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.290"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..>589
                     /locus_tag="IV041_003017"
     CDS             24..>589
                     /locus_tag="IV041_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8383705.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          209 a          101 c          116 g          163 t
ORIGIN      
        1 aaaagatagc aggaggaatg acgatgaatc attttaaagg aaagcaattt cagcaggatg
       61 tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag
      121 aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag
      181 aatatggcaa actactctat caaatttgga aaaagaaaaa taaaaaatcc ttttattcat
      241 ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca
      301 tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag
      361 cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag
      421 ataaagcccc ctctattaca agtgccttta agaaactaaa agaatacggc ttttatcaag
      481 ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag
      541 taaagagacg caataaattc tatcgaagtt tacgcactgc ctctaccac
//
LOCUS       DACDCV010000278          583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDCV010000278 DACDCV010000000
VERSION     DACDCV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.291"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..>583
                     /locus_tag="IV041_003018"
     CDS             103..>583
                     /locus_tag="IV041_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383706.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELVRQNIARFLTKLVSEWKSI
                     "
BASE COUNT          249 a           55 c           85 g          194 t
ORIGIN      
        1 aaatttctcg aaaattataa tgatttaaaa tctaccaata attatcaatt tggaaacttg
       61 tcttatgaga tgataagtaa agttctaagt ttgtgatgat aaatgaaatt aaatagtata
      121 aatgatttat ttgctattat aggtgttatt gctaacatac ttttttcgtg tttgaatttt
      181 agatttactc aaaagaacaa aaagaacaaa ctcagtatag aacaaatgaa aaaagacatt
      241 gaaactgatt taaaaaatga ccataaagaa agaagaaaaa ttgaagaata tacttcttta
      301 atggaaaagt gtaaagcaac tgatgcaatt tttagctttt taagaaaaag tgatgagata
      361 atcacacgtg aaaatataga tttagaaaat gagattagta aacttgaatc tatcttttcc
      421 gatataaatt ctcttaagta tttaatcgtc gaagaaaatt cggatagctt tttagaattt
      481 gaaaaatatc taacagatct tatagataat agaaataatg aattagtgag acaaaacatt
      541 gcaagattcc ttactaaatt agtttctgaa tggaaaagta tta
//
LOCUS       DACDCV010000279          582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDCV010000279 DACDCV010000000
VERSION     DACDCV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.292"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..308
                     /locus_tag="IV041_003019"
     CDS             <1..308
                     /locus_tag="IV041_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383707.1"
                     /translation="NQELTPLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRV
                     TGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            318..521
                     /locus_tag="IV041_003020"
     CDS             318..521
                     /locus_tag="IV041_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383708.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          236 a           85 c           95 g          166 t
ORIGIN      
        1 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
       61 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
      121 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
      181 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
      241 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
      301 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
      361 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
      421 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
      481 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
      541 tctagtcgac attctttttt tctaattgaa agtgttcata aa
//
LOCUS       DACDCV010000280          569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDCV010000280 DACDCV010000000
VERSION     DACDCV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.293"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>569
                     /locus_tag="IV041_003021"
     CDS             <1..>569
                     /locus_tag="IV041_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8383709.1"
                     /translation="VSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPDASGE
                     IADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYLV
                     ASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKDQEVE
                     AFPMSVCMSLYNKNLLDKYRLRFEKILSE"
BASE COUNT          219 a           57 c          128 g          165 t
ORIGIN      
        1 aggtaagtgt gatagtacca atttacaatg ttgaaaaata tttaagaaaa tgtgtggata
       61 gtttattgag tcagacatta aaagagatag aaatcgtatt agttgatgat ggttcgccag
      121 atgcttcggg ggaaatagca gatgagtatc aaaaaaaata ttctaatgta aaaacaatac
      181 atagagaaaa tggggggtta ggaccggcaa gaaatacagg aattgagaat gcgacaggag
      241 aatatgtggc ttttcttgat tcagatgatt gggtagaaag tgatatgtat gaaaaattat
      301 acttagttgc gtccaaagaa aatgctgata tagtagttag tggacattgt gattttgcga
      361 atgacaaagc tatggtgaaa aagcaacatc ctttgagtgg gaatgtatat gatacgccta
      421 atagtattaa aaatgtacga aaaaatttat ttggacatag tccaaaagat caagaagtag
      481 aggccttccc gatgtcagta tgtatgtctt tgtataataa aaatctgcta gataaatata
      541 gactaagatt tgaaaaaatt ttatctgaa
//
LOCUS       DACDCV010000281          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDCV010000281 DACDCV010000000
VERSION     DACDCV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.294"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..278)
                     /locus_tag="IV041_003022"
     CDS             complement(<1..278)
                     /locus_tag="IV041_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383710.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYT"
BASE COUNT          187 a           90 c           66 g          195 t
ORIGIN      
        1 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
       61 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      121 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      181 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      241 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      301 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      361 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      421 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      481 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaactttt
//
LOCUS       DACDCV010000282          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDCV010000282 DACDCV010000000
VERSION     DACDCV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.295"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..503
                     /locus_tag="IV041_003023"
     CDS             201..503
                     /locus_tag="IV041_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383711.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          173 a           78 c           86 g          193 t
ORIGIN      
        1 actattacac gtcttctgtt gtgtttgtta ttgcaggaaa tcttaatata gaaggttttc
       61 tatttgttta cattttgttg aattcgaatt tttttggcac ataatttggc acaagtactt
      121 gttttctatt attcagtata ttaaattccg ccttcacaaa aagaacattc gttcgtatac
      181 tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaaatttg
      241 acccttcgaa tagatttatc aaagattttt ataaagtatg gaattcagaa gttttctttt
      301 tagcaatcga agatagctta ttaatcaatc tctactattc taataagaac tatttcaaaa
      361 ttcctgctgc gaaaactaga atgaagaagg gtgtatactt tttgtttgat atcgtgactg
      421 acgtgccgga cgcacgtagc gatcatcggc gttatgacta tataaagtat actttcgttg
      481 atccagaaag atacaaagat taaataaaaa aataacaaac aaactcgctt
//
LOCUS       DACDCV010000283          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDCV010000283 DACDCV010000000
VERSION     DACDCV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.296"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV041_003024"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV041_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8383712.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDCV010000284          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDCV010000284 DACDCV010000000
VERSION     DACDCV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.297"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV041_003025"
     CDS             complement(<1..132)
                     /locus_tag="IV041_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8383713.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDCV010000285          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDCV010000285 DACDCV010000000
VERSION     DACDCV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.298"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IV041_003026"
     CDS             141..398
                     /locus_tag="IV041_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383714.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          209 a           65 c           70 g          152 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtcc
//
LOCUS       DACDCV010000286          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDCV010000286 DACDCV010000000
VERSION     DACDCV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.299"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IV041_003027"
     CDS             complement(62..259)
                     /locus_tag="IV041_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8383715.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDCV010000287          483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDCV010000287 DACDCV010000000
VERSION     DACDCV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.300"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            110..>483
                     /locus_tag="IV041_003028"
     CDS             110..>483
                     /locus_tag="IV041_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8383716.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGK"
BASE COUNT          184 a           66 c           89 g          144 t
ORIGIN      
        1 aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga taataggtat
       61 tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa tgagacaact
      121 acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa ttgatcatgt
      181 atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat atctatttga
      241 taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact atgctatacc
      301 aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca caacaccaga
      361 aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt gtttatctaa
      421 tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg attggggtaa
      481 aga
//
LOCUS       DACDCV010000288          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDCV010000288 DACDCV010000000
VERSION     DACDCV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.301"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          152 a           73 c           95 g          129 t
ORIGIN      
        1 tcattgcaat aattgcaaca aagaaatttc atcgggggaa gaggtttgga caaaaatgga
       61 aatttccgcc aaaagtaact gctcctcaac tgaaatcaat taaagaacta gaatttgaga
      121 atgcactggt aatttgtatg acatgtactg atgaagtatt tcgaaaacat ttttaaaggt
      181 tgagatgtat atagatttaa ccaatcattt atctctctcg gaaaaggaat attaactaac
      241 cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat ggcttatttc gcgctgatta
      301 tgagaatatc agtctgtttg attaattttt agggttcaaa atacagagac aaactccagg
      361 tcgggtttcg ccacaagcga attacccgat ctggagtttt gtctattgca ggaaggcgta
      421 gccggactgc aggaataaag cccccggtt
//
LOCUS       DACDCV010000289          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDCV010000289 DACDCV010000000
VERSION     DACDCV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.302"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..205)
                     /locus_tag="IV041_003029"
     CDS             complement(<1..205)
                     /locus_tag="IV041_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383717.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETP"
BASE COUNT          157 a           72 c           51 g          157 t
ORIGIN      
        1 taggggtttc tctttcttct ctacctagaa ggtagtctac agagactcca aagtaatcag
       61 ctaaccgagc taactcaata gaattaggag tttgtctttt ccaacttcct aaatatccat
      121 tcgaataccc gaaattcatt tcgagctgtc taatggataa tcctttttct tttgctaact
      181 cctttattat ttcgtaagta ttcattgata aatcaacctt tctgaatgct tacaaaaaaa
      241 gtttagataa ataatgtaaa aatagttgac ttatatacat tattaatcta tactatgttt
      301 tgtaaacaag tttaacaact aaaaagacaa caaaaaatac tattgattaa taaatgccaa
      361 ccgccaagaa agctttttaa atcaaagttt atatgtctta tttaactatg catttagtat
      421 agattattaa tctaata
//
LOCUS       DACDCV010000290          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDCV010000290 DACDCV010000000
VERSION     DACDCV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.303"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IV041_003030"
     CDS             <1..>404
                     /locus_tag="IV041_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383718.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDCV010000291          378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDCV010000291 DACDCV010000000
VERSION     DACDCV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.denovo.304"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..155
                     /locus_tag="IV041_003031"
     CDS             <1..155
                     /locus_tag="IV041_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP8383719.1"
                     /translation="KDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDAI
                     SYGVRK"
     gene            174..>378
                     /locus_tag="IV041_003032"
     CDS             174..>378
                     /locus_tag="IV041_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8383720.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVN"
BASE COUNT          157 a           32 c           68 g          121 t
ORIGIN      
        1 ataaggataa gtgtattaat tgtggaaaat gtaaaaaagt ttgccaaatg aataaagatt
       61 cagttgagaa ttgcaatcat ctggagtgta tcagatgtgg acgatgtaaa aatgcttgtc
      121 cagtggatgc aattagttat ggagtaagaa aatagagagg agtagttagg aaagtgataa
      181 aaaatgctat agcatatatt acaagaaaga aaaatagaac tttaataatt tttatcatat
      241 taactatagt tctttcttgc ttgtattctt gtttaacaat aatgaaatca agcaatgaaa
      301 tagaaaaggc tttatatgaa agttctaatt cttcaatatc aataacaaaa aaagatggta
      361 aatattttaa tgttaatc
//
LOCUS       DACDCV010000292         7151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.113, whole genome shotgun sequence.
ACCESSION   DACDCV010000292 DACDCV010000000
VERSION     DACDCV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.113"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..882
                     /locus_tag="IV041_003033"
     CDS             <1..882
                     /locus_tag="IV041_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8383721.1"
                     /translation="QGKNTAVIESAQALGLDLLDKPTDQTIRLASSYALGELIKDALL
                     QEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVK
                     LIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIP
                     GSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKV
                     PYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEK
                     TKILGQTLSRFFTILPK"
     gene            1140..2642
                     /locus_tag="IV041_003034"
     CDS             1140..2642
                     /locus_tag="IV041_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8383722.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2709..3236
                     /locus_tag="IV041_003035"
     CDS             2709..3236
                     /locus_tag="IV041_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8383723.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3447..4508
                     /locus_tag="IV041_003036"
     CDS             3447..4508
                     /locus_tag="IV041_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8383724.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4511..5407
                     /gene="murQ"
                     /locus_tag="IV041_003037"
     CDS             4511..5407
                     /gene="murQ"
                     /locus_tag="IV041_003037"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8383725.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5421..>7151
                     /locus_tag="IV041_003038"
     CDS             5421..>7151
                     /locus_tag="IV041_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8383726.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGL"
BASE COUNT         2383 a         1259 c         1624 g         1885 t
ORIGIN      
        1 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
       61 aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa
      121 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
      181 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
      241 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
      301 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
      361 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
      421 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
      481 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
      541 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
      601 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
      661 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
      721 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
      781 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
      841 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
      901 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
      961 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     1021 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     1081 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     1141 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     1201 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     1261 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     1321 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     1381 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     1441 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     1501 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     1561 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     1621 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     1681 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     1741 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     1801 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     1861 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     1921 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     1981 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     2041 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     2101 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     2161 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     2221 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     2281 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     2341 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     2401 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     2461 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     2521 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     2581 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     2641 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     2701 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     2761 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     2821 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     2881 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     2941 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     3001 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     3061 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     3121 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     3181 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     3241 gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt
     3301 ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta
     3361 ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa
     3421 gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt
     3481 tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt
     3541 tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac
     3601 taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat
     3661 tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag
     3721 gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca catcaattga
     3781 aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag
     3841 aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc
     3901 tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc
     3961 aaggttttgt accaggagat acagagttga gaggcccact atatcaagga ttgccaactt
     4021 tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca
     4081 cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt
     4141 ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg
     4201 aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg
     4261 tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga
     4321 aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa
     4381 ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag
     4441 agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag
     4501 gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga
     4561 aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga
     4621 tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa
     4681 cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg
     4741 aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat
     4801 ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga
     4861 agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat
     4921 agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc
     4981 aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa
     5041 acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg
     5101 attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat
     5161 tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact
     5221 aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga
     5281 aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa
     5341 tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac
     5401 cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac
     5461 aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc
     5521 gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc
     5581 caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag
     5641 tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt
     5701 cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac
     5761 gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg
     5821 tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga
     5881 tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag
     5941 caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc
     6001 tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacacca actcttggtg
     6061 gagttatcgg ggccgttgtg atgctgacag ggatgaatcc ggaagcacca ctgaaaaact
     6121 tatttactgg tgaagctctt gcagctggac aaggtgggat cctaggtgtc atcttcgctg
     6181 tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata
     6241 tcatcgttac accaactctt tcattgattg ccatcggtct gattgaaatt ttcttcatca
     6301 tgcctttagc aggtttcatt tctgatggca tggtcggtgg gatcaactgg gtacttggcg
     6361 ttggtggagc atttgccggc ttcgttctag gcacattatt cttgccgatg gttatgttcg
     6421 gactgcatca aattttgact ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc
     6481 tattgcttcc aattcttgcg atggctggtg caggacaagt gggtgcagca ttggcattat
     6541 ggattcgctg caaaaaagat aaagaattaa cagaaatgat caaaggagct ttaccagtcg
     6601 ggatcctagg aatcggcgaa ccattgattt acggagtaac attacctcta ggacgcccat
     6661 ttattactgc ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg atcggaaatg
     6721 tcggggctat cgctattgga ccttcaggag ccgctttgat cccattgatc tctgacggta
     6781 aatggttagg ctacatcctt ggattattgg cagcttatgc tggtggcttc gttgctacat
     6841 tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga attagcgact gaaacagcca
     6901 cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt gtgactatcc
     6961 tctcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgat ccagtttttg
     7021 cacaaaagat gatgggtgaa ggctactttg tagaaccaga aaacggacat atctattctc
     7081 cagttactgg ggtagtcagc tctgtcttcc caacgaaaca cgctattggg atcacaacgc
     7141 caaatggttt a
//
LOCUS       DACDCV010000293         5258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.127, whole genome shotgun sequence.
ACCESSION   DACDCV010000293 DACDCV010000000
VERSION     DACDCV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.127"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..748
                     /locus_tag="IV041_003039"
     CDS             188..748
                     /locus_tag="IV041_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8383727.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            745..2637
                     /locus_tag="IV041_003040"
     CDS             745..2637
                     /locus_tag="IV041_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8383728.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2644..3021
                     /locus_tag="IV041_003041"
     CDS             2644..3021
                     /locus_tag="IV041_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8383729.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3175..3957
                     /locus_tag="IV041_003042"
     CDS             3175..3957
                     /locus_tag="IV041_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8383730.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4083..4814)
                     /gene="erm(A)"
                     /locus_tag="IV041_003043"
     CDS             complement(4083..4814)
                     /gene="erm(A)"
                     /locus_tag="IV041_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8383731.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(4872..4959)
                     /locus_tag="IV041_003044"
                     /pseudo
     CDS             complement(4872..4959)
                     /locus_tag="IV041_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
BASE COUNT         1890 a          731 c          998 g         1639 t
ORIGIN      
        1 gagttttctt gattatttaa gtagattagg agaatttaaa tcaattgatg tatttaaaca
       61 agcaaaggga agaaatttca aaggattttt acatcatgtt aataagggta gataccaaaa
      121 gaatgtctta aagttaaggg ttaaaaagaa acagataaga acattgagat cagaggaagt
      181 taagcaaatt attgatgctt gtcatacgaa aagagataaa ttaattttaa tgcttatgta
      241 tgagggtggt ttaagaatcg gtgaagtgtt atcgcttagg cttgaagata ttgtcacttg
      301 ggacaatcaa atccatttaa cacctagaga tgttaatgtt aatgaagctt atattaaatt
      361 aaggaaggaa agaacaatac atgtgagtaa agaacttatg tcactttata cagattactt
      421 gatatatgag tatagtgagg aattggagca tgattatgtt tttatttcct taaaagaagg
      481 ctattttggg aaaccactaa agtaccaaag tgttcttgat ctagttagaa gaatagttaa
      541 aaggactgga atagaattta catcacatat gcttcgccac actcacgcaa cgcagctaat
      601 tagggaagga tgggatgttg cgttcgttca aaagagatta ggtcacgcac atgttcagac
      661 aacgttaaat acctatgttc atctttcaga tcaggatatg aaaaatgagt ttaataaata
      721 cctcgagaga aaggagcata agaaatgaat gcttctagta aaaggaaaat tattagtcag
      781 agtgagatta gcaaaaaaat agctgtaatg aatgaagaaa tgcaggggtt ttgggctaat
      841 aatagttggg atataagaaa atgtccacat ccttctgcca tagaattaag taagaatcct
      901 gctttaagga atcgttgggt tcgttttgaa cgtgttaaaa atctgtggtt aagaacagaa
      961 ttgaaatatt tttattttta ccatttaaac aatggaatat ggaatgcaaa aactgtctgg
     1021 attagaaaag gaacagtaat taataaaatg ttagatttct tagatttaaa gtatcctagc
     1081 attacttcaa ttactgaagt tcctattgaa aaagcaatga cggagtatag aacttatttg
     1141 acaaaacggg gtgttagaat taccactact aattataaga ttactgctaa tcaagaaaaa
     1201 acacctgtaa aagctaattc ctactatgtt actaatctaa aacaatttat ggagttttat
     1261 gagaactttt attttgatgg agaggagtgg gataaagacg tttgggatag acgtaactta
     1321 cctttgccag atgataaggt taacccaaca caatatgaat atacaattaa ctttaaaggg
     1381 tttcggaata catattttaa acaacttgta aaaagatatt gtaagttgag attgaacatg
     1441 gatagctttt cctatgtaag tgatattgcc caaagactta aagagttctt taattttctg
     1501 gacatgaaat ttaaacaagt tcagagagta caccaattaa cgagagtgga aattgaagca
     1561 tatttaagtg aactaaacat gatgggaata aaacctagta caataactgg gaggatctct
     1621 atattggaag gactatttag tacccttctt aggctagaat gggatgatgt tccttccaaa
     1681 atattaattt attctgagga ctatccgaaa ataccaagag caaaaccacg ctttatagat
     1741 gaattcgtcc tagagcaatt gaacagtcat cttgataaat tacccgaata tatagctacg
     1801 atgactatga ttgttcaaga atgtggaatg aggataagtg aattgtgcac cttgaaaaaa
     1861 ggctgtctat tagaggacaa agatggagat ttctttttaa agtattatca atggaaaatg
     1921 aaaaaggagc atatagttcc aatatctaaa gaggtagctt tacttattaa agttcgggaa
     1981 gataaagttt cagaggaatt tccagatagt gaatacctct ttccaagaaa agatggatcg
     2041 ccattaaaac aagaaacatt tagaggtgag ttaaataaat tagcttatga gcaaaatata
     2101 gtggataaat caggtgagat ttatagattc catgcccatg cctttcgcca tacagtagga
     2161 acaagaatga ttaacaacgg gatgccccag catattgtgc agaaattttt ggggcatgaa
     2221 agcccagaaa tgacaagcag atacgctcat atctttgatg aaactctaaa aaatgaattt
     2281 actaaatttc aggaaaaact ggttaccaat aatggagatg tgcttgatct agatgaagat
     2341 aatgaagtcg atgatgtaga gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt
     2401 ccaaatggtt attgtagatt gccagtagta gcaggtggtt gtccacatgc gaatgcatgc
     2461 ttagattgca ctcacttctg taccagtaag caattcttac cacagcacga agaacagtta
     2521 gagcgtacag aagagttatt agccatagct aaggataaac aatggcaaag acaagtagag
     2581 actaatagcc gtgttaaaga gcgtttagaa caaatcattg gaagtttgac ggggtaatta
     2641 tcaatggata aacaagttag aaatacaaca gaaattgtac gtttggcgaa gcagaaatca
     2701 aaaaagacaa gggaaaaagt agacaaagcg atttctaaat tttcgattga aggtaaagtt
     2761 attaatttta attcaatagc aaaggaagct aatgtttcta aatcatggct ttataaggaa
     2821 cacgatatta ggcaaagaat cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc
     2881 tcaaaaccca agaaaagttc tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga
     2941 gtaatggaat tagaaaaaga aaataaaaaa ttacagaacc aaattcaaaa attatatgga
     3001 gatctgtata ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa
     3061 acgctaacgg tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta
     3121 aattgagtat caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc
     3181 aatttgatta acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat
     3241 ttatttggaa gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta
     3301 cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact
     3361 cgaaaaaaac taacagaaag actaatgact atatcaggaa agattggaaa tacggattct
     3421 gttagaccac ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct
     3481 ccaaaaagag aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt
     3541 caggaaccaa gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt
     3601 atttctctat ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt
     3661 cgaagagcaa ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt
     3721 aatgtaattt taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg
     3781 aaagatgtcg ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg
     3841 gatatagcta gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca
     3901 aaggtgaaag cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct
     3961 aattttattg caataacagg tgcttacttt taaaactact gatttattga taaatattga
     4021 acaatttttg ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta
     4081 atttagtgaa acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta
     4141 ttaatattag tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga
     4201 acacgatatt cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc
     4261 tttgaaatca atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta
     4321 ggatgaaaat atagtggtgg tacttttttg agcattttta tatccatctc caccattaat
     4381 agtaaaccca aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata
     4441 agatagctat atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg
     4501 ttataaggaa tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga
     4561 atatccgttt gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga
     4621 cataagcctc catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg
     4681 gtaaaatgtc cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg
     4741 tgattcaata tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg
     4801 tttttctggt tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc
     4861 attataacca attaattttg atttggttga taacgaactt tattaataac aaatatagaa
     4921 aaagtaccca tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt
     4981 tctactactg cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg
     5041 tatcatcttt tcattttaga ataaaccttt atatgtaatt ttgcacttga cacttcagat
     5101 ggacgttttc ttcaaaataa tatgttttga aacttgataa gagaagccac tgtctttact
     5161 aaaaagtaaa ggctgcgata aaggacagtg actttaataa agttgcactg ttgtattgtc
     5221 tttggataaa attgtctaaa gggaagtttt atccccaa
//
LOCUS       DACDCV010000294         4358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.144, whole genome shotgun sequence.
ACCESSION   DACDCV010000294 DACDCV010000000
VERSION     DACDCV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.144"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            308..1786
                     /gene="msr"
                     /locus_tag="IV041_003045"
     CDS             308..1786
                     /gene="msr"
                     /locus_tag="IV041_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8383732.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1833..2279
                     /locus_tag="IV041_003046"
     CDS             1833..2279
                     /locus_tag="IV041_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8383733.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2478..2993)
                     /locus_tag="IV041_003047"
     CDS             complement(2478..2993)
                     /locus_tag="IV041_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8383734.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3110..4312
                     /locus_tag="IV041_003048"
     CDS             3110..4312
                     /locus_tag="IV041_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8383735.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1313 a          730 c          945 g         1370 t
ORIGIN      
        1 gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt
       61 cgaacttttg aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg
      121 atggagtatg tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg
      181 tgatttagtc atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa
      241 acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt
      301 aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca
      361 gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg
      421 agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga
      481 tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc
      541 tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac
      601 ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc
      661 ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat
      721 tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga
      781 tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg
      841 agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca
      901 gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga
      961 aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat
     1021 cagactggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa
     1081 gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca
     1141 gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat
     1201 catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt
     1261 tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac
     1321 cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt
     1381 tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt
     1441 catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt
     1501 tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct
     1561 gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa
     1621 ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac
     1681 gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga
     1741 aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga
     1801 ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa
     1861 tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc
     1921 tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg
     1981 ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac
     2041 cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag
     2101 gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat
     2161 tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt
     2221 tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat
     2281 ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca
     2341 gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc
     2401 ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga
     2461 aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac
     2521 cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat
     2581 tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg
     2641 ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa
     2701 aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg
     2761 atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag
     2821 tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta
     2881 tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca
     2941 gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc
     3001 ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc
     3061 atattgacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt
     3121 ggagaatcaa atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc
     3181 gctagttgta gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat
     3241 cttaaaaacg attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac
     3301 tagttacatg ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat
     3361 cggtttgaaa aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg
     3421 cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa
     3481 tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga
     3541 aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt
     3601 cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat
     3661 gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa
     3721 agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact
     3781 aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt
     3841 gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc
     3901 tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat
     3961 tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat
     4021 gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac
     4081 actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag
     4141 ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg
     4201 tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt
     4261 atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag
     4321 aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DACDCV010000295         3742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.160, whole genome shotgun sequence.
ACCESSION   DACDCV010000295 DACDCV010000000
VERSION     DACDCV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.160"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..1178
                     /gene="rlmN"
                     /locus_tag="IV041_003049"
     CDS             111..1178
                     /gene="rlmN"
                     /locus_tag="IV041_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8383736.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1371..2474)
                     /locus_tag="IV041_003050"
     CDS             complement(1371..2474)
                     /locus_tag="IV041_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8383737.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3027..3575
                     /gene="aac(6')"
                     /locus_tag="IV041_003051"
     CDS             3027..3575
                     /gene="aac(6')"
                     /locus_tag="IV041_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8383738.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1228 a          634 c          755 g         1125 t
ORIGIN      
        1 tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat
       61 gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac
      121 agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga
      181 aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca
      241 cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca
      301 atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg
      361 aactccctga taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag
      421 tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc
      481 tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac
      541 attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg
      601 gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca
      661 aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa
      721 tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga
      781 ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga
      841 tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca
      901 tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag
      961 ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc
     1021 aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg
     1081 gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat
     1141 tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca
     1201 attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa
     1261 gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga
     1321 aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt
     1381 ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc
     1441 tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg
     1501 gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat
     1561 actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga
     1621 tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc
     1681 attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta
     1741 ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca
     1801 attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag
     1861 agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca
     1921 tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc
     1981 cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt
     2041 ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc
     2101 gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt
     2161 ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg
     2221 tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta
     2281 cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg
     2341 ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct
     2401 ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa
     2461 acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga
     2521 gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga
     2581 tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta
     2641 caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc
     2701 ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg
     2761 ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg
     2821 aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa
     2881 gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac
     2941 tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga
     3001 ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag
     3061 tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca
     3121 gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag
     3181 accaagatga gttagtagga tttattggtg caatccctca atacggtatc acaggttggg
     3241 aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag
     3301 tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg
     3361 atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata
     3421 aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt
     3481 ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg
     3541 caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa
     3601 tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt tgtgaaattc
     3661 caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg tgaaaaagtc
     3721 gcttagtttc gagtagtaag aa
//
LOCUS       DACDCV010000296         3122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.171, whole genome shotgun sequence.
ACCESSION   DACDCV010000296 DACDCV010000000
VERSION     DACDCV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.171"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IV041_003052"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IV041_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IV041_003053"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IV041_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8383739.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IV041_003054"
     CDS             1851..3005
                     /locus_tag="IV041_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8383740.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          965 a          612 c          641 g          904 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 ga
//
LOCUS       DACDCV010000297         3055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.173, whole genome shotgun sequence.
ACCESSION   DACDCV010000297 DACDCV010000000
VERSION     DACDCV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.173"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            246..743
                     /gene="dfrG"
                     /locus_tag="IV041_003055"
     CDS             246..743
                     /gene="dfrG"
                     /locus_tag="IV041_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8383741.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(815..2767)
                     /locus_tag="IV041_003056"
     CDS             complement(815..2767)
                     /locus_tag="IV041_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8383742.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2774..3010)
                     /locus_tag="IV041_003057"
     CDS             complement(2774..3010)
                     /locus_tag="IV041_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8383743.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          939 a          530 c          513 g         1073 t
ORIGIN      
        1 acaaaatgta cttcaaaatg gactactttt ttcattttaa cttacatacc aaattacata
       61 caaaatgatt tataaaatgc aattgaaaat gataattgaa tgaaaatttg tgattttgtg
      121 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
      181 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      241 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      301 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      361 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      421 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      481 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      541 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      601 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      661 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      721 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      781 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      841 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      901 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
      961 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
     1021 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
     1081 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1141 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1201 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1261 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1321 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1381 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1441 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1501 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1561 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1621 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1681 aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt
     1741 ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac
     1801 ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt
     1861 cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt
     1921 agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg
     1981 tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt
     2041 aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa
     2101 ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt
     2161 ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt
     2221 tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt
     2281 cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga
     2341 tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac
     2401 agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt
     2461 tatctttgga actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt
     2521 tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg
     2581 gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg
     2641 ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt
     2701 ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct
     2761 tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat
     2821 tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga
     2881 attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca
     2941 cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa
     3001 gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaa
//
LOCUS       DACDCV010000298         2903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.178, whole genome shotgun sequence.
ACCESSION   DACDCV010000298 DACDCV010000000
VERSION     DACDCV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.178"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1106
                     /locus_tag="IV041_003058"
     CDS             138..1106
                     /locus_tag="IV041_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8383744.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1099..2130
                     /gene="vanA"
                     /locus_tag="IV041_003059"
     CDS             1099..2130
                     /gene="vanA"
                     /locus_tag="IV041_003059"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8383745.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2136..2744
                     /locus_tag="IV041_003060"
     CDS             2136..2744
                     /locus_tag="IV041_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8383746.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          859 a          558 c          714 g          772 t
ORIGIN      
        1 aaacggctcg ttcttacacg gtagacttaa taccgtaaga acgagccgtt ttcgttcttc
       61 agagaaagat ttgacaagat taccattggc atccccgttt tatttggtgc ctttcacaga
      121 aagggttggt cttaattatg aataacatcg gcattactgt ttatggatgt gagcaggatg
      181 aggcagatgc attccatgct ctttcgcctc gctttggcgt tatggcaacg ataattaacg
      241 ccaacgtgtc ggaatccaac gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac
      301 ataaatcaga gatttccgcc tctattcttc ttgcgctgaa gagagccggt gtgaaatata
      361 tttctacccg aagcatcggc tgcaatcata tagatacaac tgctgctaag agaatgggca
      421 tcactgtcga caatgtggcg tactcgccgg atagcgttgc cgattatact atgatgctaa
      481 ttcttatggc agtacgcaac gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca
      541 ggttggacag cgaccgtggc aaggtactca gcgacatgac agttggtgtg gtgggaacgg
      601 gccagatagg caaagcggtt attgagcggc tgcgaggatt tggatgtaaa gtgttggctt
      661 atagtcgcag ccgaagtata gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata
      721 gcgatatcgt tacgcttcat gtgccgctca atacggatac gcactatatt atcagccacg
      781 aacaaataca gagaatgaag caaggagcat ttcttatcaa tactgggcgc ggtccacttg
      841 tagataccta tgagttggtt aaagcattag aaaacgggaa actgggcggt gccgcattgg
      901 atgtattgga aggagaggaa gagtttttct actctgattg cacccaaaaa ccaattgata
      961 atcaattttt acttaaactt caaagaatgc ctaacgtgat aatcacaccg catacggcct
     1021 attataccga gcaagcgttg cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt
     1081 ttgaaaggag acaggagcat gaatagaata aaagttgcaa tactgtttgg gggttgctca
     1141 gaggagcatg acgtatcggt aaaatctgca atagagatag ccgctaacat taataaagaa
     1201 aaatacgagc cgttatacat tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa
     1261 ccttgcgcgg aatgggaaaa cgacaattgc tattcagctg tactctcgcc ggataaaaaa
     1321 atgcacggat tacttgttaa aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca
     1381 ttttcagctt tgcatggcaa gtcaggtgaa gatggatcca tacaaggtct gtttgaattg
     1441 tccggtatcc cttttgtagg ctgcgatatt caaagctcag caatttgtat ggacaaatcg
     1501 ttgacataca tcgttgcgaa aaatgctggg atagctactc ccgccttttg ggttattaat
     1561 aaagatgata ggccggtggc agctacgttt acctatcctg tttttgttaa gccggcgcgt
     1621 tcaggctcat ccttcggtgt gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt
     1681 gaatcggcaa gacaatatga cagcaaaatc ttaattgagc aggctgtttc gggctgtgag
     1741 gtcggttgtg cggtattggg aaacagtgcc gcgttagttg ttggcgaggt ggaccaaatc
     1801 aggctgcagt acggaatctt tcgtattcat caggaagtcg agccggaaaa aggctctgaa
     1861 aacgcagtta taaccgttcc cgcagacctt tcagcagagg agcgaggacg gatacaggaa
     1921 acggcaaaaa aaatatataa agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt
     1981 ttacaagata acggccgcat tgtactgaac gaagtcaata ctctgcccgg tttcacgtca
     2041 tacagtcgtt atccccgtat gatggccgct gcaggtattg cacttcccga actgattgac
     2101 cgcttgatcg tattagcgtt aaaggggtga taagcatgga aataggattt acttttttag
     2161 atgaaatagt acacggtgtt cgttgggacg ctaaatatgc cacttgggat aatttcaccg
     2221 gaaaaccggt tgacggttat gaagtaaatc gcattgtagg gacatacgag ttggctgaat
     2281 cgcttttgaa ggcaaaagaa ctggctgcta cccaagggta cggattgctt ctatgggacg
     2341 gttaccgtcc taagcgtgct gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata
     2401 acctgacaaa ggaaagttat tatcccaata ttgaccgaac tgagatgatt tcaaaaggat
     2461 acgtggcttc aaaatcaagc catagccgcg gcagtgccat tgatcttacg ctttatcgat
     2521 tagacacggg tgagcttgta ccaatgggga gccgatttga ttttatggat gaacgctctc
     2581 atcatgcggc aaatggaata tcatgcaatg aagcgcaaaa tcgcagacgt ttgcgctcca
     2641 tcatggaaaa cagtgggttt gaagcatata gcctcgaatg gtggcactat gtattaagag
     2701 acgaaccata ccccaatagc tattttgatt tccccgttaa ataaactttt aaccgttgca
     2761 cggacaaact atataagcta actctttcgg caggaaaccc gacgtatgta actggttctt
     2821 agggaattta tatatagtag atagtattga agatgtaagg cagagggttc tgttgcaaag
     2881 ttttaaataa agaataaaat ccc
//
LOCUS       DACDCV010000299         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.204, whole genome shotgun sequence.
ACCESSION   DACDCV010000299 DACDCV010000000
VERSION     DACDCV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.204"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..1126
                     /locus_tag="IV041_003061"
     CDS             86..1126
                     /locus_tag="IV041_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8383747.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1224..1412
                     /locus_tag="IV041_003062"
     CDS             1224..1412
                     /locus_tag="IV041_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8383748.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1470..1823)
                     /locus_tag="IV041_003063"
     CDS             complement(1470..1823)
                     /locus_tag="IV041_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8383749.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          661 a          320 c          360 g          662 t
ORIGIN      
        1 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
       61 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
      121 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
      181 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
      241 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
      301 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
      361 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
      421 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
      481 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
      541 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
      601 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
      661 cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtacggttt
      721 atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta ttgtactgga
      781 gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaagttta
      841 tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt gtgcaaatat
      901 cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc ctgctcgatg
      961 tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg tttgtttagc
     1021 agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa
     1081 tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg ttttatgttg
     1141 ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata gatttgaatt
     1201 aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt cctatttgtg
     1261 gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc cctttatact
     1321 gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata ataacaatca
     1381 aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct catcttatcg
     1441 gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc ttcattgata
     1501 ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc catgtatttt
     1561 tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa aaattcatca
     1621 atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg gtgttgtcct
     1681 ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc cacttgttca
     1741 cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg
     1801 tatttatctt cttttttacg catactaaat cacctctata cattttactg ttcctatata
     1861 accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata gaacactgtg
     1921 gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt tatttaccta
     1981 tacaacttta taaaatttag tca
//
LOCUS       DACDCV010000300         1896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.208, whole genome shotgun sequence.
ACCESSION   DACDCV010000300 DACDCV010000000
VERSION     DACDCV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.208"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..550
                     /locus_tag="IV041_003064"
     CDS             23..550
                     /locus_tag="IV041_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8383750.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(613..1266)
                     /locus_tag="IV041_003065"
     CDS             complement(613..1266)
                     /locus_tag="IV041_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8383751.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1398..>1896
                     /locus_tag="IV041_003066"
     CDS             1398..>1896
                     /locus_tag="IV041_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8383752.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNL"
BASE COUNT          704 a          346 c          373 g          473 t
ORIGIN      
        1 tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt
       61 gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca
      121 aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg
      181 tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg
      241 cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat
      301 ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt
      361 ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg
      421 gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc
      481 gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt
      541 gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct
      601 ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat
      661 aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc
      721 gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga
      781 gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt
      841 agatactttt gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag
      901 aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag
      961 caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac
     1021 ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa
     1081 taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa
     1141 gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc
     1201 atgggtgact gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt
     1261 ttccatgaaa acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta
     1321 tactaaaaat agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga
     1381 aagagagaga gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg
     1441 aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg
     1501 gaacagtata tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga
     1561 aagcaaaaaa tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg
     1621 atgagctagg agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg
     1681 gaacagtaga agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg
     1741 acagtgatac cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa
     1801 aagaaggtaa aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa
     1861 aattatacat cggaatatcc acactagata acttgg
//
LOCUS       DACDCV010000301         1638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.217, whole genome shotgun sequence.
ACCESSION   DACDCV010000301 DACDCV010000000
VERSION     DACDCV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.217"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..273
                     /locus_tag="IV041_003067"
     CDS             <1..273
                     /locus_tag="IV041_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8383753.1"
                     /translation="AYCYTLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSK
                     EIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            274..>1638
                     /gene="aph(2'')-I"
                     /locus_tag="IV041_003068"
     CDS             274..>1638
                     /gene="aph(2'')-I"
                     /locus_tag="IV041_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP8383754.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          678 a          128 c          281 g          551 t
ORIGIN      
        1 gcatattgct atacactttt aagacctgat ggaaaaacaa tgttttattt acactcaata
       61 ggaatgttac ctaactatca agacaaaggt tatggttcaa aattattatc ttttattaag
      121 gaatattcta aagagattgg ttgttctgaa atgtttttaa taactgataa aggtaatcct
      181 agagcttgcc atgtatatga aaaattaggt ggtaaaaatg attataaaga tgaaatagta
      241 tatgtatatg attatgaaaa aggtgataaa taaatgaata tagttgaaaa tgaaatatgt
      301 ataagaactt taatagatga tgattttcct ttgatgttaa aatggttaac tgatgaaaga
      361 gtattagaat tttatgatgg tagagataaa aaatatacat tagaatcatt aaaaaaacat
      421 tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa caatgttcct
      481 attggatatg gacaaatata taaaatgtat gatgagttat atactgatta tcattatcca
      541 aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa ttattggagt
      601 aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat
      661 gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa
      721 aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa
      781 gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg
      841 aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt
      901 agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt
      961 agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca
     1021 aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta
     1081 tctatactag gttataaaga aattaaagga acttttttaa caccagaaat ttattctact
     1141 atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg
     1201 cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta
     1261 gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat
     1321 tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt
     1381 ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact
     1441 ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt tatatactta
     1501 cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat
     1561 ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct
     1621 attgaaacta ttgtttat
//
LOCUS       DACDCV010000302         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.226, whole genome shotgun sequence.
ACCESSION   DACDCV010000302 DACDCV010000000
VERSION     DACDCV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.226"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IV041_003069"
                     /pseudo
     CDS             262..373
                     /locus_tag="IV041_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IV041_003070"
     CDS             394..477
                     /locus_tag="IV041_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8383755.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IV041_003071"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IV041_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8383756.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDCV010000303         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.234, whole genome shotgun sequence.
ACCESSION   DACDCV010000303 DACDCV010000000
VERSION     DACDCV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.234"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV041_003072"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV041_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV041_003073"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV041_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8383757.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDCV010000304         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20117_2016
            SAMN12640424-rid9892933.guided.235, whole genome shotgun sequence.
ACCESSION   DACDCV010000304 DACDCV010000000
VERSION     DACDCV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640424
            Sequence Read Archive: SRR10041136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743080.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743080.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:27:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,009
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 40 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 8 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640424-rid9892933.guided.235"
                     /isolate="E20117_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IV041_003074"
     CDS             <1..1311
                     /locus_tag="IV041_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP8383758.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          566 a          102 c          227 g          442 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattat
//
